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18 results on '"Qiu, Wuxia"'

7. BPAG1, a distinctive role in skin and neurological diseases

Author

Qiu Wuxia, Xiaoli Ma, Fan Zhao, Yan Zhang, Lifang Hu, Pai Wang, Lei Chen, Airong Qian, and Arshad Ali

Subjects
0301 basic medicine, Cytoskeleton organization, Dystonin, Biology, Skin Diseases, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Cell polarity, medicine, Animals, Humans, Protein Isoforms, Tissue Distribution, Cytoskeleton, Cell adhesion, integumentary system, Alternative splicing, Cell migration, Cell Biology, medicine.disease, Cell biology, 030104 developmental biology, Immunology, Epidermolysis bullosa, Nervous System Diseases, Developmental Biology
Abstract

Spectraplakins are multifunctional cytoskeletal linker proteins that act as important communicators, connecting cytoskeletal components with each other and to cellular junctions. Bullous pemphigoid antigen 1 (BPAG1)/dystonin is a member of spectraplakin family and expressed in various tissues. Alternative splicing of BPAG1 gene produces various isoforms with unique structure and domains. BPAG1 plays crucial roles in numerous biological processes, such as cytoskeleton organization, cell polarization, cell adhesion, and cell migration as well as signaling transduction. Genetic mutation of BPAG1 isoforms is the miscreant of epidermolysis bullosa and multifarious, destructive neurological diseases. In this review, we summarize the recent advances of BPAG1's role in various biological processes and in skin and neurological diseases.

Published
2017

8. Mesenchymal MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation

Author

Zhao, Fan, primary, Ma, Xiaoli, additional, Qiu, Wuxia, additional, Wang, Pai, additional, Zhang, Ru, additional, Chen, Zhihao, additional, Su, Peihong, additional, Zhang, Yan, additional, Li, Dijie, additional, Ma, Jianhua, additional, Yang, Chaofei, additional, Chen, Lei, additional, Yin, Chong, additional, Tian, Ye, additional, Hu, Lifang, additional, Li, Yu, additional, Zhang, Ge, additional, Wu, Xiaoyang, additional, and Qian, Airong, additional

Published
2020
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10. Circulating miR-181c-5p and miR-497-5p Are Potential Biomarkers for Prognosis and Diagnosis of Osteoporosis

Author

Ma, Jianhua, primary, Lin, Xiao, primary, Chen, Chu, primary, Li, Siyu, primary, Zhang, Shasha, primary, Chen, Zhihao, primary, Li, Dijie, primary, Zhao, Fan, primary, Yang, Chaofei, primary, Yin, Chong, primary, Qiu, Wuxia, primary, Xiao, Yunyun, primary, Zhang, Kewen, primary, Miao, Zhiping, primary, Yang, Tuanmin, primary, and Qian, Airong, primary

Published
2019
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12. MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation in Mice

Author

Zhao, Fan, primary, Ma, Xiaoli, additional, Qiu, Wuxia, additional, Wang, Pai, additional, Zhang, Ru, additional, Chen, Zhihao, additional, Su, Peihong, additional, Zhang, Yan, additional, Li, Dijie, additional, Ma, Jianhua, additional, Yang, Chaofei, additional, Chen, Lei, additional, Yin, Chong, additional, Tian, Ye, additional, Hu, Lifang, additional, Li, Yu, additional, Zhang, Ge, additional, Wu, Xiaoyang, additional, and Qian, Airong, additional

Published
2019
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14. Phosphate glass fibers facilitate proliferation and osteogenesis through Runx2 transcription in murine osteoblastic cells.

Author

Lin, Xiao, Chen, Qiang, Xiao, Yunyun, Gao, Yongguang, Ahmed, Ifty, Li, Meng, Li, Hui, Zhang, Kewen, Qiu, Wuxia, Liu, Xianhu, Boccaccini, Aldo R., and Qian, Airong

Abstract

Cell–material interactions and compatibility are important aspects of bioactive materials for bone tissue engineering. Phosphate glass fiber (PGF) is an attractive inorganic filler with fibrous structure and tunable composition, which has been widely investigated as a bioactive filler for bone repair applications. However, the interaction of osteoblasts with PGFs has not been widely investigated to elucidate the osteogenic mechanism of PGFs. In this study, different concentrations of short PGFs with interlaced oriented topography were cocultured with MC3T3‐E1 cells for different periods, and the synergistic effects of fiber topography and ionic product of PGFs on osteoblast responses including cell adhesion, spreading, proliferation, and osteogenic differentiation were investigated. It was found that osteoblasts were more prone to adhere on PGFs through Vinculin protein, leading to enhanced cell proliferation with polygonal cell shape and spreading cellular actin filaments. In addition, osteoblasts incubated on PGF meshes showed enhanced alkaline phosphatase activity, extracellular matrix mineralization, and increased expression of osteogenesis‐related marker genes, which could be attributed to the Wnt/β‐catenin/Runx2 signaling pathway. This study elucidated the possible mechanism of PGF on triggering specific osteoblast behavior, which would be highly beneficial for designing PGF‐based bone graft substitutes with excellent osteogenic functions. [ABSTRACT FROM AUTHOR]

Published
2020
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15. MACF1 Overexpression by Transfecting the 21 kbp Large Plasmid PEGFP-C1A-ACF7 Promotes Osteoblast Differentiation and Bone Formation

Author

Zhang, Yan, primary, Yin, Chong, additional, Hu, Lifang, additional, Chen, Zhihao, additional, Zhao, Fan, additional, Li, Dijie, additional, Ma, Jianhua, additional, Ma, Xiaoli, additional, Su, Peihong, additional, Qiu, Wuxia, additional, Yang, Chaofei, additional, Wang, Pai, additional, Li, Siyu, additional, Zhang, Ge, additional, Wang, Liping, additional, Qian, Airong, additional, and Xian, Cory J., additional

Published
2018
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18. MACF1 Overexpression by Transfecting the 21 kbp Large Plasmid PEGFP-C1A-ACF7 Promotes Osteoblast Differentiation and Bone Formation

Author

Airong Qian, Lifang Hu, Zhihao Chen, Liping Wang, Chong Yin, Wang Pai, Cory J. Xian, Chaofei Yang, Dijie Li, Ge Zhang, Peihong Su, Yan Zhang, Wuxia Qiu, Jianhua Ma, Li Siyu, Xiaoli Ma, Fan Zhao, Zhang, Yan, Yin, Chong, Hu, Lifang, Chen, Zhihao, Zhao, Fan, Li, Dijie, Ma, Jianhua, Ma, Xiaoli, Su, Peihong, Qiu, Wuxia, Yang, Chaofei, Wang, Pai, Li, Siyu, Zhang, Ge, Wang, Liping, Qian, Airong, and Xian, Cory J.

Subjects
0301 basic medicine, Transfection, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Osteogenesis, Gene expression, Genetics, medicine, Animals, Humans, Cytoskeleton, Molecular Biology, beta Catenin, bone formation, Osteoblasts, Chemistry, Electroporation, Microfilament Proteins, Skull, Gene Expression Regulation, Developmental, Cell Differentiation, Cell migration, Osteoblast, Cell biology, 030104 developmental biology, medicine.anatomical_structure, MACF1, 030220 oncology & carcinogenesis, osteoblast, Molecular Medicine, Alkaline phosphatase, large plasmid, Plasmids, Signal Transduction
Abstract

Microtubule actin crosslinking factor 1 (MACF1) is a large spectraplakin protein known to have crucial roles in regulating cytoskeletal dynamics, cell migration, growth, and differentiation. However, its role and action mechanism in bone remain unclear. The present study investigated optimal conditions for effective transfection of the large plasmid PEGFP-C1A-ACF7 (∼21 kbp) containing full-length human MACF1 cDNA, as well as the potential role of MACF1 in bone formation. To enhance MACF1 expression, the plasmid was transfected into osteogenic cells by electroporation in vitro and into mouse calvaria with nanoparticles. Then, transfection efficiency, osteogenic marker expression, calvarial thickness, and bone formation were analyzed. Notably, MACF1 overexpression triggered a drastic increase in osteogenic gene expression, alkaline phosphatase activity, and matrix mineralization in vitro. Mouse calvarial thickness, mineral apposition rate, and osteogenic marker protein expression were significantly enhanced by local transfection. In addition, MACF1 overexpression promoted β-catenin expression and signaling. In conclusion, MACF1 overexpression by transfecting the large plasmid containing full-length MACF1 cDNA promotes osteoblast differentiation and bone formation via β-catenin signaling. Current data will provide useful experimental parameters for the transfection of large plasmids and a novel strategy based on promoting bone formation for prevention and therapy of bone disorders. Refereed/Peer-reviewed

Published
2018

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