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6 results on '"Qiulong Zhao"'

1. Pharmacokinetic Study of Coadministration with Cefuroxime Sodium for Injection Influencing ReDuNing Injection-Derived Seven Phytochemicals and Nine Metabolites in Rats

Author

Qiulong Zhao, Chunxue Wang, Jiaxin Cheng, Hui Yan, Ling Wang, Dawei Qian, and Jinao Duan

Subjects
Analytical chemistry, QD71-142
Abstract

According to the sixth edition of China’s “New Coronavirus Diagnosis and Treatment Plan (NCDTP),” ReDuNing injection (RDN) was firstly introduced to treat severe and critical COVID-19, whereas its combination with broad-spectrum antibiotics was suggested to take with extreme caution and full reasons. Therefore, we aim to describe the pharmacokinetics of seven active phytochemicals and semiquantification of nine relevant metabolites in ReDuNing injection (RDN) after combining with cefuroxime sodium (CNa) for injection in rat plasma. Male Sprague–Dawley rats were randomly assigned to six groups, and they were intravenously administered, respectively, with different prescriptions of RDN (2 mL/kg) and CNa (225 mg/kg). At different time points (0.03, 0.08, 0.17, 0.24, 0.33, 0.50, 0.67, 1, and 6 h) after administration, the drug concentrations of iridoids glycosides, organic acids, and metabolites in rat plasma were determined using ultrahigh-pressure liquid chromatography coupled with linear ion rap-orbitrap tandem mass spectrometry (UHPLC–LTQ–Orbitrap–MS), and main pharmacokinetic parameters were estimated by noncompartment model. The results showed that there were differences in pharmacokinetic parameters, AUC(0-t), T1/2, Cmax, CL of iridoids glycosides, and organic acids, after the intravenous administration of the different combinations of RDN and CNa. Moreover, different combinations of the injections also resulted in different curves of relative changes of each metabolite. The obtained results suggested that RDN and CNa existed pharmacokinetic drug–herb interactions in rats. The findings not only lay the foundation for evaluating the safety of RDN injection combined with CNa but also make contributions to clinically applying RDN injection combined with CNa, which works potentially against severe forms of COVID-19.

Published
2022
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3. Identification of absorbed compounds of Xiao Yao San Jia Wei and pharmacokinetic study in depressed rats by force swimming stress

Author

Chenxiao, Shan, Jia, Li, Bo, Sun, Runze, Zhou, Min, Xu, Qiulong, Zhao, Ping, Ren, Hongmei, Wen, and Xi, Huang

Subjects
General Chemical Engineering, General Chemistry
Abstract

Xiao-Yao-San-Jia-Wei (XYSJW) is a commonly prescribed formulation for depression and anorexia in the Jiang Su Province Hospital of Chinese Medicine. Unfortunately, the proper dosage of this formulation is still unclear due to its limited chemical and pharmacokinetic profiles. Thus, in the present study, a sensitive, precise, and rapid procedure for the identification of absorbed compounds (Cs) in the plasma of depressed rats together with a pharmacokinetic analysis was established with the help of ultra-flow liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UFLC-Q-TOF MS/MS) and ultra-flow liquid chromatography coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-QQQ MS/MS). Based on the characteristic fragmentation, neutral loss, mass defect filter, relevant literature and reference standards, 225 Cs in the XYSJW extract and 20 Cs in the plasma of the depressed rats were tentatively recognized

Published
2022

4. Pharmacokinetics-derived absorbed components responsible for Guizhi-Fuling capsule target PI3K/Akt-Erk to exert an anti-dysmenorrhea effect

Author

Qiulong Zhao, Jiaxin Cheng, Xiaokun Bian, Chunxue Wang, Yi Xu, Hongxiang Ding, Hui Ren, Yiying Zhang, Min Xu, Chenxiao Shan, Hui Yan, Jinao Duan, Dawei Qian, and Xi Huang

Subjects
Pharmacology, Phosphatidylinositol 3-Kinases, Dysmenorrhea, Tandem Mass Spectrometry, Drug Discovery, Prostaglandins F, Animals, Humans, Female, Proto-Oncogene Proteins c-akt, Chromatography, Liquid, Drugs, Chinese Herbal, Rats
Abstract

Guizhi-Fuling capsule (GZFL), a well-known herbal remedy, has been widely used to treat primary dysmenorrhea (PD). Hence, systematic identifying multiple active ingredients and the involved mechanism is essential and urgently needed for GZFL.This study was planned to assess the pharmacokinetics of GZFL in rats, and identify whether these GZFL-derived absorbed components (ACs) contribute to the efficacy of source herbs and relevant mechanism.The in vivo pharmacokinetic profile of 11 phytochemicals and 13 metabolites in healthy and PD rats were evaluated using liquid chromatography with mass spectrometry (LC-MS/MS). Whereafter, the introduced contribution strategy assessed ACs' effect (doses = their contents in GZFL) in PD rats with the mechanism.The pharmacokinetic profiles of prototypes and metabolites differed in healthy and PD rats. As a main proxy of GZFL, 11ACs exerted an anti-PD effect (improvement of indexes for writhing latency, writhing time, PGFAs a paradigmatic example, 11ACs contributed an average of 113.55% to GZFL in terms of anti-PD efficacy, providing an approach to rapidly, accurately and consistently identify the bioactive components and their pathway from herbs.

Published
2022

6. Letter to the Editor in response to the articles ‘Lianhuaqingwen exerts anti-viral and anti-inflammatory activities against novel coronavirus (SARS-CoV-2)’ and ‘Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-κB signaling pathway.’

Author

Shaoqi Shi, Xi Huang, Liang Xia, and Qiulong Zhao

Subjects
GAPDH, glyceraldehyde 3-phosphate dehydrogenase, Anti-Inflammatory Agents, G-CSF, Granulocyte colony-stimulating factor, DMSO, dimethyl sulfoxide, RT-PCR, real-time polymerase chain reaction, TNF-α, tumor necrosis factor-α, Medicine, COVID-19, 2019 coronavirus disease, MOI, multiplicity of infection, anti-inflammatory, LS, Liu Shen capsule, CXCL-10/IP-10, interferon-inducible protein-10, TCID50, 100 × 50% tissue culture infective dose, NF-kappa B, MERS-CoV, Middle East respiratory syndrome coronavirus, antiviral, TC50, 50% toxicity concentration, CPE, cytopathic effect, FBS, foetal bovine serum, EC50, 50% effective concentration, Severe acute respiratory syndrome coronavirus, TCM, traditional Chinese medicine, MIP1a, Recombinant Macrophage Inflammatory Protein 1 Alpha, MTT, Methyl thiazolyl tetrazolium, Signal Transduction, IL-8, Interleukin-8, VERO, E6 cells Monkey kidney cell line, 2019-20 coronavirus outbreak, Coronavirus disease 2019 (COVID-19), Liu Shen capsule, medicine.drug_class, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), SARS-CoV, severe acute respiratory syndrome coronavirus, PBS, phosphate-buffered saline, Antiviral Agents, Article, Anti-inflammatory, Humans, IL-1β, Interleukin-1beta, IL-6, Interleukin-6, ComputingMethodologies_COMPUTERGRAPHICS, Pharmacology, SARS-CoV-2, business.industry, Lianhuaqingwen, COVID-19, Virology, Nf κb signaling, CCL-2/MCP-1, monocyte chemoattracctant protein-1, SARS-CoV-2, Severe Acute Respiratory Syndrome Coronavirus 2, HPLC, high-performance liquid chromatography, business, Drugs, Chinese Herbal
Abstract

Graphical abstract, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide through person-to-person contact, causing a public health emergency of international concern. At present, there is no specific antiviral treatment recommended for SARS-CoV-2 infection. Liu Shen capsule (LS), a traditional Chinese medicine, has been proven to have a wide spectrum of pharmacological properties, such as anti-inflammatory, antiviral and immunomodulatory activities. However, little is known about the antiviral effect of LS against SARS-CoV-2. Herein, the study was designed to investigate the antiviral activity of SARS-CoV-2 and its potential effect in regulating the host’s immune response. The inhibitory effect of LS against SARS-CoV-2 replication in Vero E6 cells was evaluated by using the cytopathic effect (CPE) and plaque reduction assay. The number of virions of SARS-CoV-2 was observed under transmission electron microscope after treatment with LS. Proinflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. The results showed that LS could significantly inhibit SARS-CoV-2 replication in Vero E6 cells, and reduce the number of virus particles and it could markedly reduce pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, IL-8, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Moreover, the expression of the key proteins in the NF-κB/MAPK signaling pathway was detected by western blot and it was found that LS could inhibit the expression of p-NF-κB p65, p-IκBα and p-p38 MAPK, while increasing the expression of IκBα. These findings indicate that LS could inhibit SARS-CoV-2 virus infection via downregulating the expression of inflammatory cytokines induced virus and regulating the activity of NF-κB/MAPK signaling pathway in vitro, making its promising candidate treatment for controlling COVID-19 disease.

Published
2021

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