Search

The molecular mechanism that promotes pancreatic cancer remains unclear, so it is important to find the molecular network of important genes related to pancreatic cancer. To find the key molecule of pancreatic cancer, differential gene expression analyses were analyzed by the Deseq2 package, edgeR package, and limma-voom package, respectively. Pancreatic cancer survival-related genes were analyzed by COX survival analysis. Finally, we integrated the results to obtain the significantly differentially expressed gene, MYEOV (myeloma overexpressed gene), most strongly related to survival in pancreatic cancer. Experimental verification by qRT-PCR confirmed that transcription levels of MYEOV mRNA markedly increased in pancreatic cancer cells relative to normal human pancreatic ductal epithelial cells (HPDE). Through the comprehensive analysis of multiple databases, we constructed a molecular network centered on MYEOV and found specific links between molecules in this network and tumor-associated immune cells. It was noted that MYEOV could serve as a ceRNA by producing molecular sponging effects on hsa-miR-103a-3p and hsa-miR-107, thus affecting the role of GPRC5A, SERPINB5, EGFR, KRAS, EIF4G2, and PDCD4 on pancreatic cancer progression. Besides, we also identified that infiltrated immune cells are potential mediators for the molecules in the MYEOV-related network to promote pancreatic cancer progression. It is the first report to focus on the possibility that MYEOV may act as a competing endogenous RNA (ceRNA) to form an interactive network with some pancreatic cancer-related genes such as KRAS and serve as a key therapeutic target of pancreatic cancer treatment.

Calliphora vomitoria is a significant insect which belongs to the Calliphoridae family. In this study, the mitochondrial genome of C. vomitoria was completely sequenced for species identification. The entire mitogenome was 16,134 bp in length, composing of 13 protein-encoding genes, 22 transfer RNA genes and two ribosomal RNA genes, and then the array of the genes was similar to the other insects have discovered. The overall base compositions of A, G, C and T were 39.40%, 9.37%, 13.08% and 37.13% respectively. What is more, phylogenetic analyses tree indicated that entire mitochondria genome sequences of C. vomitoria had high degree of identification among the species listed in. We hope that the results from the present study will provide useful dipteran mitochondrial genomes information for the further studies on genetic structure and phylogenetic analyses of C. vomitoria in the species identifications.

Magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites were prepared via the solution combustion process, their structure and magnetic properties were investigated by XRD, SEM, TEM and VSM. The asprepared magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites were characterized with the average grain size of about 20 nm, the specific magnetization of 31.6 Am2/kg, and the specific surface area of 117.0 m2/g. The adsorption kinetics of bovine serum albumin (BSA) from aqueous solution onto the magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites at room temperature was investigated. The kinetics data related to the adsorption of BSA from aqueous solutions were in good agreement with the pseudo-second-order kinetics model in the initial concentrations of 0.2-0.8 mg/mL.

Magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites were prepared via the solution combustion process, their structure and magnetic properties were investigated by XRD, SEM, TEM and VSM. The asprepared magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites were characterized with the average grain size of about 20 nm, the specific magnetization of 31.6 Am2/kg, and the specific surface area of 117.0 m2/g. The adsorption kinetics of bovine serum albumin (BSA) from aqueous solution onto the magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites at room temperature was investigated. The kinetics data related to the adsorption of BSA from aqueous solutions were in good agreement with the pseudo-second-order kinetics model in the initial concentrations of 0.2-0.8 mg/mL.

Calliphora vomitoria is a significant insect which belongs to the Calliphoridae family. In this study, the mitochondrial genome of C. vomitoria was completely sequenced for species identification. The entire mitogenome was 16,134 bp in length, composing of 13 protein-encoding genes, 22 transfer RNA genes and two ribosomal RNA genes, and then the array of the genes was similar to the other insects have discovered. The overall base compositions of A, G, C and T were 39.40%, 9.37%, 13.08% and 37.13% respectively. What is more, phylogenetic analyses tree indicated that entire mitochondria genome sequences of C. vomitoria had high degree of identification among the species listed in. We hope that the results from the present study will provide useful dipteran mitochondrial genomes information for the further studies on genetic structure and phylogenetic analyses of C. vomitoria in the species identifications.