25 results on '"Quévrain, E."'
Search Results
2. Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii, a commensal bacterium deficient in Crohn’s disease
- Author
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Quévrain, E, Maubert, M A, Michon, C, Chain, F, Marquant, R, Tailhades, J, Miquel, S, Carlier, L, Bermúdez-Humarán, L G, Pigneur, B, Lequin, O, Kharrat, P, Thomas, G, Rainteau, D, Aubry, C, Breyner, N, Afonso, C, Lavielle, S, Grill, J-P, Chassaing, G, Chatel, J M, Trugnan, G, Xavier, R, Langella, P, Sokol, H, and Seksik, P
- Published
- 2016
- Full Text
- View/download PDF
3. Gut microbiota: Description, role and pathophysiologic implications
- Author
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Landman, C., Quévrain, E., CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Inflammation intestinale, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Gastroentérologie et nutrition [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)
- Subjects
Microbiote ,Immune system ,Maladies inflammatoires chroniques intestinales ,Dysbiose ,Système immunitaire ,Dysbiosis ,Gut microbiota ,Obesity ,Obésité ,Inflammatory bowel diseases ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; The human gut contains 1014 bacteria and many other micro-organisms such as Archaea, viruses and fungi. Studying the gut microbiota showed how this entity participates to gut physiology and beyond this to human health, as a real “hidden organ”. In this review, we aimed to bring information about gut microbiota, its structure, its roles and its implication in human pathology. After bacterial colonization in infant, intestinal microbial composition is unique for each individual although more than 95% can be assigned to four major phyla. The use of culture independent methods and more recently the development of high throughput sequencing allowed to depict precisely gut microbiota structure and diversity as well as its alteration in diseases. Gut microbiota is implicated in the maturation of the host immune system and in many fundamental metabolic pathways including sugars and proteins fermentation and metabolism of bile acids and xenobiotics. Imbalance of gut microbial populations or dysbiosis has important functional consequences and is implicated in many digestive diseases (inflammatory bowel diseases, colorectal cancer, etc.) but also in obesity and autism. These observations have led to a surge of studies exploring therapeutics which aims to restore gut microbiota equilibrium such as probiotics or fecal microbiota transplantation. But recent research also investigates biological activity of microbial products which could lead to interesting therapeutics leads.; Le microbiote intestinal humain est composé de 1014 bactéries ainsi que d’autres micro-organismes comme les virus, les champignons et les archées. L’étude du microbiote intestinal a dévoilé le rôle fondamental qu’il joue dans la physiologie intestinale mais aussi dans la santé humaine de façon plus générale, comme un véritable« organe caché ». Dans cette revue, nous exposons la structure et le rôle du microbiote intestinal ainsi que son implication en pathologie humaine. Après la colonisation bactérienne du tube digestif chez le nourrisson, la composition du microbiote intestinal est unique à chaque individu bien que plus de 95 % des bactéries le composant puissent être réparties en 4 phyla majeurs. Des approches culture-indépendantes et, plus récemment, l’avènement du séquençage haut débit ont permis de décrire précisément la structure et la diversité du microbiote intestinal ainsi que son altération en pathologie. Le microbiote intestinal est impliqué dans la maturation du système immunitaire et dans de nombreuses voies métaboliques fondamentales comme la fermentation des sucres et des protéines ainsi que le métabolisme des acides biliaires et des xénobiotiques. Le déséquilibre des populations du microbiote intestinal ou dysbiose a des conséquences fonctionnelles importantes et est impliqué dans de nombreuses pathologies digestives (maladies inflammatoires chroniques intestinales, cancer colorectal, etc.) mais aussi dans l’obésité et l’autisme. Ces observations ont conduit à l’émergence de nombreuses études sur les traitements visant à restaurer l’équilibre du microbiote intestinal comme les probiotiques ou la transplantation du microbiote fécal. Mais des travaux récents sur l’activité de métabolites issus du microbiote pourraient conduire à des perspectives thérapeutiques prometteuses.
- Published
- 2016
4. Assessing the potential bacterial origin of the chemical diversity in calcareous sponges
- Author
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Quévrain, E., Roué, Mélanie, Domart-Coulon, I., and Bourguet-Kondracki, M.L.
- Subjects
ACTIVITE BIOLOGIQUE ,BENTHOS ,BACTERIOLOGIE ,ANTAGONISME ,SPONGIAIRE - Published
- 2014
5. Le microbiote intestinal : description, rôle et implication physiopathologique
- Author
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Landman, C., primary and Quévrain, E., additional
- Published
- 2016
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- View/download PDF
6. Identification of an anti-inflammatory protein fromFaecalibacterium prausnitzii, a commensal bacterium deficient in Crohn’s disease
- Author
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Quévrain, E, primary, Maubert, M A, additional, Michon, C, additional, Chain, F, additional, Marquant, R, additional, Tailhades, J, additional, Miquel, S, additional, Carlier, L, additional, Bermúdez-Humarán, L G, additional, Pigneur, B, additional, Lequin, O, additional, Kharrat, P, additional, Thomas, G, additional, Rainteau, D, additional, Aubry, C, additional, Breyner, N, additional, Afonso, C, additional, Lavielle, S, additional, Grill, J-P, additional, Chassaing, G, additional, Chatel, J M, additional, Trugnan, G, additional, Xavier, R, additional, Langella, P, additional, Sokol, H, additional, and Seksik, P, additional
- Published
- 2015
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7. OP018 Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii, a deficient commensal bacteria implicated in Crohn's disease
- Author
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Quévrain, E., primary, Maubert, M.-A., additional, Chain, F., additional, Marquant, R., additional, Kharrat, P., additional, Carlier, L., additional, Bermudez, L., additional, Pigneur, B., additional, Lequin, O., additional, Bridonneau, C., additional, Thomas, G., additional, Lavielle, S., additional, Grill, J.-P., additional, Chassaing, G., additional, Masliah, J., additional, Trugnan, G., additional, Xavier, R., additional, Langella, P., additional, Sokol, H., additional, and Seksik, P., additional
- Published
- 2014
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- View/download PDF
8. DOP015 Gut microbiota can induce autophagy in intestinal epithelial cells, a concern for Crohn's disease
- Author
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Tran-Minh, M.-L., primary, Quévrain, E., additional, Brot, L., additional, Thomas, G., additional, Grill, J.-P., additional, Trugnan, G., additional, and Seksik, P., additional
- Published
- 2014
- Full Text
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9. Novel natural parabens produced by a Microbulbifer bacterium in its calcareous sponge host Leuconia nivea
- Author
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Quévrain, E, Domart-Coulon, I, Pernice, M, Bourguet-Kondracki, ML, Quévrain, E, Domart-Coulon, I, Pernice, M, and Bourguet-Kondracki, ML
- Abstract
A broad variety of natural parabens, including four novel structures and known ethyl and butyl parabens, were obtained from culture of a Microbulbifer sp. bacterial strain isolated from the temperate calcareous marine sponge Leuconia nivea (Grant 1826). Their structures were elucidated from spectral analysis, including mass spectrometry and 1D and 2D nuclear magnetic resonance. Their antimicrobial activity evaluated against Staphylococcus aureus was characterized by much higher in vitro activity of these natural paraben compounds 3-9 than commercial synthetic methyl and propyl parabens, usually used as antimicrobial preservatives. Compounds 4 and 9 revealed a bacteriostatic effect and compounds 6 and 7 appeared as bactericidal compounds. Major paraben compound 6 was also active against Gram positive Bacillus sp. and Planococcus sp. sponge isolates and was detected in whole sponge extracts during all seasons, showing its persistent in situ production within the sponge. Moreover, Microbulbifer sp. bacteria were visualized in the sponge body wall using fluorescence in situ hybridization with a probe specific to L4-n2 phylotypes. Co-detection in the sponge host of both paraben metabolites and Microbulbifer sp. L4-n2 indicates, for the first time, production of natural parabens in a sponge host, which may have an ecological role as chemical mediators. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.
- Published
- 2009
10. Faecalibacterium prausnitzii : recherche des molécules bioactives anti-inflammatoires
- Author
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Pigneur, B., primary, Quévrain, E., additional, Maubert, M.-A., additional, Tailhades, J., additional, Sokol, H., additional, and Seksik, P., additional
- Published
- 2013
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11. Deciphering the Metal Speciation in Low-Molecular-Weight Complexes by IMS-MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates.
- Author
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Zoumpoulaki M, Schanne G, Delsuc N, Preud'homme H, Quévrain E, Eskenazi N, Gazzah G, Guillot R, Seksik P, Vinh J, Lobinski R, and Policar C
- Subjects
- Ion Mobility Spectrometry, Mass Spectrometry, Metals, Molecular Weight, Superoxide Dismutase metabolism, Coordination Complexes, Manganese chemistry
- Abstract
The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. Mn
II complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13 C6 analogue., (© 2022 Wiley-VCH GmbH.)- Published
- 2022
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12. Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models.
- Author
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Vincent A, Thauvin M, Quévrain E, Mathieu E, Layani S, Seksik P, Batinic-Haberle I, Vriz S, Policar C, and Delsuc N
- Subjects
- Animals, Antioxidants metabolism, Cell Line, Glutathione Peroxidase metabolism, Humans, Hydrogen Peroxide metabolism, Metalloporphyrins metabolism, Molecular Mimicry, Oxidation-Reduction, Oxidative Stress, Porphyrins metabolism, Reactive Oxygen Species metabolism, Superoxides metabolism, Catalase metabolism, Manganese metabolism, Organometallic Compounds metabolism, Superoxide Dismutase metabolism
- Abstract
Oxidative stress that results from an imbalance between the concentrations of reactive species (RS) and antioxidant defenses is associated with many pathologies. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase are among the key enzymes that maintain the low nanomolar physiological concentrations of superoxide and hydrogen peroxide. The increase in the levels of these species and their progeny could have deleterious effects. In this context, chemists have developed SOD and CAT mimics to supplement them when cells are overwhelmed with oxidative stress. However, the beneficial activity of such molecules in cells depends not only on their intrinsic catalytic activities but also on their stability in biological context, their cell penetration and their cellular localization. We have employed cellular assays to characterize several compounds that possess SOD and CAT activities and have been frequently used in cellular and animal models. We used cellular assays that address SOD and CAT activities of the compounds. Finally, we determined the effect of compounds on the suppression of the inflammation in HT29-MD2 cells challenged by lipopolysaccharide. When the assay requires penetration inside cells, the SOD mimics Mn(III) meso-tetrakis(N-(2'-n-butoxyethyl)pyridinium-2-yl)porphyrin (MnTnBuOE-2-PyP
5+ ) and Mn(II) dichloro[(4aR,13aR,17aR,21aR)-1,2,3,4,4a,5,6,12,13,13a,14,15,16,17,17a,18,19,20,21,21a-eicosahydro-11,7-nitrilo-7Hdibenzo[b,h] [1,4, 7,10] tetraazacycloheptadecine-κN5,κN13,κN18,κN21,κN22] (Imisopasem manganese, M40403, CG4419) were found efficacious at 10 μM, while Mn(II) chloro N-(phenolato)-N,N'-bis[2-(N-methyl-imidazolyl)methyl]-ethane-1,2-diamine (Mn1) requires an incubation at 100 μM. This study thus demonstrates that MnTnBuOE-2-PyP5+ , M40403 and Mn1 were efficacious in suppressing inflammatory response in HT29-MD2 cells and such action appears to be related to their ability to enter the cells and modulate reactive oxygen species (ROS) levels., (Copyright © 2021 Elsevier Inc. All rights reserved.)- Published
- 2021
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13. Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(ii) superoxide dismutase mimic complex.
- Author
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Mathieu E, Bernard AS, Quévrain E, Zoumpoulaki M, Iriart S, Lung-Soong C, Lai B, Medjoubi K, Henry L, Nagarajan S, Poyer F, Scheitler A, Ivanović-Burmazović I, Marco S, Somogyi A, Seksik P, Delsuc N, and Policar C
- Subjects
- Anti-Inflammatory Agents, Non-Steroidal chemistry, Anti-Inflammatory Agents, Non-Steroidal metabolism, Coordination Complexes chemistry, Coordination Complexes metabolism, Humans, Molecular Structure, Oxidative Stress drug effects, Rhenium chemistry, Rhenium metabolism, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Coordination Complexes pharmacology, Rhenium pharmacology, Superoxide Dismutase metabolism
- Abstract
A conjugate of a Mn-based superoxide dismutase mimic with a Re-based multimodal probe 1[combining low line] was studied in a cellular model of oxidative stress. Its speciation was investigated using Re and Mn X-fluorescence. Interestingly, 1[combining low line] shows a distribution different from its unconjugated analogue but a similar concentration in mitochondria and a similar bioactivity.
- Published
- 2020
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14. An easy-to-implement combinatorial approach involving an activity-based assay for the discovery of a peptidyl copper complex mimicking superoxide dismutase.
- Author
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Vincent A, Fores JR, Tauziet E, Quévrain E, Dancs Á, Conte-Daban A, Bernard AS, Pelupessy P, Coulibaly K, Seksik P, Hureau C, Selmeczi K, Policar C, and Delsuc N
- Subjects
- Amino Acid Sequence, Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Colon cytology, Colon drug effects, Colon metabolism, Copper metabolism, HT29 Cells, Humans, Interleukin-8 metabolism, Lipopolysaccharides toxicity, Oxidative Stress drug effects, Peptides metabolism, Superoxide Dismutase metabolism, Biocompatible Materials metabolism, Copper chemistry, Peptides chemistry
- Abstract
A combinatorial approach using a one-bead-one-compound method and a screening based on a SOD-activity assay was set up for the discovery of an efficient peptidyl copper complex. The complex exhibited good stability constants, suitable redox potentials and excellent intrinsic activity. This complex was further assayed in cells for its antioxidant properties and showed beneficial effects when cells were subjected to oxidative stress.
- Published
- 2020
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15. Inter-kingdom effect on epithelial cells of the N-Acyl homoserine lactone 3-oxo-C12:2, a major quorum-sensing molecule from gut microbiota.
- Author
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Landman C, Grill JP, Mallet JM, Marteau P, Humbert L, Le Balc'h E, Maubert MA, Perez K, Chaara W, Brot L, Beaugerie L, Sokol H, Thenet S, Rainteau D, Seksik P, and Quévrain E
- Subjects
- Acyl-Butyrolactones chemistry, Acyl-Butyrolactones metabolism, Caco-2 Cells, Cell Communication genetics, Epithelial Cells metabolism, Epithelial Cells microbiology, Escherichia coli genetics, Escherichia coli isolation & purification, Escherichia coli metabolism, Feces microbiology, Gene Expression Regulation, Bacterial, Humans, Inflammatory Bowel Diseases genetics, Inflammatory Bowel Diseases metabolism, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa isolation & purification, Signal Transduction, Acyl-Butyrolactones isolation & purification, Gastrointestinal Microbiome genetics, Inflammatory Bowel Diseases microbiology, Quorum Sensing genetics
- Abstract
Background and Aims: N-acyl homoserine lactones (AHLs), which are autoinducer quorum-sensing molecules involved in the bacterial communication network, also interact with eukaryotic cells. Searching for these molecules in the context of inflammatory bowel disease (IBD) is appealing. The aims of our study were to look for AHL molecules in faecal samples from healthy subjects (HS) and IBD patients to correlate AHL profiles with the microbiome and investigate the effect of AHLs of interest on epithelial cells., Methods: Using mass spectrometry, we characterised AHL profiles in faecal samples from HS (n = 26) and IBD patients in remission (n = 24) and in flare (n = 25) and correlated the presence of AHLs of interest with gut microbiota composition obtained by real-time qPCR and 16S sequencing. We synthesised AHLs of interest to test the inflammatory response after IL1β stimulation and paracellular permeability on Caco-2 cells., Results: We observed 14 different AHLs, among which one was prominent. This AHL corresponded to 3-oxo-C12:2 and was found significantly less frequently in IBD patients in flare (16%) and in remission (37.5%) versus HS (65.4%) (p = 0.001). The presence of 3-oxo-C12:2 was associated with significantly higher counts of Firmicutes, especially Faecalbacterium prausnitzii, and lower counts of Escherichia coli. In vitro, 3-oxo-C12:2 exerted an anti-inflammatory effect on Caco-2 cells. Interestingly, although 3-oxo-C12, the well-known AHL from Pseudomonas aeruginosa, increased paracellular permeability, 3-oxo-C12:2 did not., Conclusions: We identified AHLs in the human gut microbiota and discovered a new and prominent AHL, 3-oxo-C12:2, which correlates with normobiosis and exerts a protective effect on gut epithelial cells., Competing Interests: Dr Landman has received personal fees from Abbvie and Hospira and travel support from Abbvie, Hospira, Mayoly Spindler, Biocodex and Takeda. Professor Marteau has received lecture fees from Abbvie, Astellas, Biocodex, Danone, Hospira-Pfizer, Janssen, Merck-MSD, Ferring Pharmaceuticals and Takeda. Professor Beaugerie has received consulting fees from Janssen; lecture fees from Abbvie, Janssen, MSD, Ferring Pharmaceuticals and Takeda; and research support from Abbvie, Ferring Pharmaceuticals, Hospira-Pfizer, Janssen and Takeda. Professor Sokol has received personal fees from Danone, MSD, Takeda, Abbvie, Astellas, BMS and Novartis; options from Enterome and Maat; and grants from Biocodex. Professor Seksik has received personal fees from Takeda, Merck MSD, Biocodex and Abbvie and non-financial support from Takeda. The remaining authors disclose no conflict. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2018
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16. A Cell-Penetrant Manganese Superoxide Dismutase (MnSOD) Mimic Is Able To Complement MnSOD and Exerts an Antiinflammatory Effect on Cellular and Animal Models of Inflammatory Bowel Diseases.
- Author
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Mathieu E, Bernard AS, Delsuc N, Quévrain E, Gazzah G, Lai B, Chain F, Langella P, Bachelet M, Masliah J, Seksik P, and Policar C
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal chemistry, Anti-Inflammatory Agents, Non-Steroidal metabolism, Cell Survival drug effects, Cells, Cultured, Chemokines antagonists & inhibitors, Chemokines metabolism, Colitis chemically induced, Colitis metabolism, Dinitrofluorobenzene analogs & derivatives, Lipopolysaccharides antagonists & inhibitors, Lipopolysaccharides pharmacology, Male, Mice, Mice, Inbred C57BL, Molecular Structure, Organometallic Compounds chemistry, Organometallic Compounds metabolism, Oxidative Stress drug effects, Superoxide Dismutase chemistry, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Colitis drug therapy, Disease Models, Animal, Inflammatory Bowel Diseases drug therapy, Organometallic Compounds pharmacology, Superoxide Dismutase metabolism
- Abstract
Inorganic complexes are increasingly used for biological and medicinal applications, and the question of the cell penetration and distribution of metallodrugs is key to understanding their biological activity. Oxidative stress is known to be involved in inflammation and in inflammatory bowel diseases for which antioxidative defenses are weakened. We report here the study of the manganese complex Mn1 mimicking superoxide dismutase (SOD), a protein involved in cell protection against oxidative stress, using an approach in inorganic cellular chemistry combining the investigation of Mn1 intracellular speciation using mass spectrometry and of its quantification and distribution using electron paramagnetic resonance and spatially resolved X-ray fluorescence with evaluation of its biological activity. More precisely, we have looked for and found the MS signature of Mn1 in cell lysates and quantified the overall manganese content. Intestinal epithelial cells activated by bacterial lipopolysaccharide were taken as a cellular model of oxidative stress and inflammation. DNBS-induced colitis in mice was used to investigate Mn1 activity in vivo. Mn1 exerts an intracellular antiinflammatory activity, remains at least partially coordinated, with diffuse distribution over the whole cell, and functionally complements mitochondrial MnSOD.
- Published
- 2017
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17. [Gut microbiota: Description, role and pathophysiologic implications].
- Author
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Landman C and Quévrain E
- Subjects
- Dysbiosis metabolism, Dysbiosis microbiology, Dysbiosis pathology, Humans, Immune System microbiology, Infant, Newborn, Inflammatory Bowel Diseases metabolism, Inflammatory Bowel Diseases microbiology, Inflammatory Bowel Diseases pathology, Intestinal Mucosa metabolism, Obesity metabolism, Obesity microbiology, Dysbiosis complications, Gastrointestinal Microbiome physiology, Intestines microbiology, Intestines pathology
- Abstract
The human gut contains 10(14) bacteria and many other micro-organisms such as Archaea, viruses and fungi. Studying the gut microbiota showed how this entity participates to gut physiology and beyond this to human health, as a real "hidden organ". In this review, we aimed to bring information about gut microbiota, its structure, its roles and its implication in human pathology. After bacterial colonization in infant, intestinal microbial composition is unique for each individual although more than 95% can be assigned to four major phyla. The use of culture independent methods and more recently the development of high throughput sequencing allowed to depict precisely gut microbiota structure and diversity as well as its alteration in diseases. Gut microbiota is implicated in the maturation of the host immune system and in many fundamental metabolic pathways including sugars and proteins fermentation and metabolism of bile acids and xenobiotics. Imbalance of gut microbial populations or dysbiosis has important functional consequences and is implicated in many digestive diseases (inflammatory bowel diseases, colorectal cancer, etc.) but also in obesity and autism. These observations have led to a surge of studies exploring therapeutics which aims to restore gut microbiota equilibrium such as probiotics or fecal microbiota transplantation. But recent research also investigates biological activity of microbial products which could lead to interesting therapeutics leads., (Copyright © 2015 Société Nationale Française de Médecine Interne (SNFMI). Published by Elsevier SAS. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
18. The presence of the anti-inflammatory protein MAM, from Faecalibacterium prausnitzii, in the intestinal ecosystem.
- Author
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Quévrain E, Maubert MA, Sokol H, Devreese B, and Seksik P
- Subjects
- Animals, Humans, Male, Bacterial Proteins metabolism, Clostridiales metabolism, Crohn Disease microbiology, Dysbiosis microbiology, Intestinal Mucosa microbiology
- Published
- 2016
- Full Text
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19. CD4CD8αα lymphocytes, a novel human regulatory T cell subset induced by colonic bacteria and deficient in patients with inflammatory bowel disease.
- Author
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Sarrabayrouse G, Bossard C, Chauvin JM, Jarry A, Meurette G, Quévrain E, Bridonneau C, Preisser L, Asehnoune K, Labarrière N, Altare F, Sokol H, and Jotereau F
- Subjects
- CD4 Antigens metabolism, CD8 Antigens metabolism, CD8-Positive T-Lymphocytes immunology, Colon immunology, Colon microbiology, Colonic Neoplasms immunology, Forkhead Transcription Factors biosynthesis, Humans, Interleukin-10 biosynthesis, Intestinal Mucosa immunology, T-Lymphocyte Subsets immunology, Clostridium immunology, Colitis, Ulcerative immunology, Crohn Disease immunology, Intestinal Mucosa cytology, T-Lymphocytes, Regulatory immunology
- Abstract
How the microbiota affects health and disease is a crucial question. In mice, gut Clostridium bacteria are potent inducers of colonic interleukin (IL)-10-producing Foxp3 regulatory T cells (Treg), which play key roles in the prevention of colitis and in systemic immunity. In humans, although gut microbiota dysbiosis is associated with immune disorders, the underlying mechanism remains unknown. In contrast with mice, the contribution of Foxp3 Treg in colitis prevention has been questioned, suggesting that other compensatory regulatory cells or mechanisms may exist. Here we addressed the regulatory role of the CD4CD8 T cells whose presence had been reported in the intestinal mucosa and blood. Using colonic lamina propria lymphocytes (LPL) and peripheral blood lymphocytes (PBL) from healthy individuals, and those with colon cancer and irritable bowel disease (IBD), we demonstrated that CD4CD8αα (DP8α) T lymphocytes expressed most of the regulatory markers and functions of Foxp3 Treg and secreted IL-10. Strikingly, DP8α LPL and PBL exhibited a highly skewed repertoire toward the recognition of Faecalibacterium prausnitzii, a major Clostridium species of the human gut microbiota, which is decreased in patients with IBD. Furthermore, the frequencies of DP8α PBL and colonic LPL were lower in patients with IBD than in healthy donors and in the healthy mucosa of patients with colon cancer, respectively. Moreover, PBL and LPL from most patients with active IBD failed to respond to F. prausnitzii in contrast to PBL and LPL from patients in remission and/or healthy donors. These data (i) uncover a Clostridium-specific IL-10-secreting Treg subset present in the human colonic LP and blood, (ii) identify F. prausnitzii as a major inducer of these Treg, (iii) argue that these cells contribute to the control or prevention of colitis, opening new diagnostic and therapeutic strategies for IBD, and (iv) provide new tools to address the systemic impact of both these Treg and the intestinal microbiota on the human immune homeostasis., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2014
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20. High-resolution mass spectrometry and partial de novo sequencing constitute a useful approach for determining the profile of chemokine secretion following the stimulation of human intestinal epithelial cells.
- Author
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Maubert MA, Quévrain E, Capton E, Grill JP, Thomas G, Bachelet M, Rainteau D, Trugnan G, Tabet JC, Masliah J, and Afonso C
- Subjects
- Chemokine CXCL1 analysis, Chemokine CXCL1 chemistry, Chemokine CXCL1 metabolism, Chemokines chemistry, Chemokines metabolism, Dose-Response Relationship, Immunologic, Enzyme-Linked Immunosorbent Assay, Epithelial Cells drug effects, HT29 Cells, Humans, Interleukin-8 analysis, Interleukin-8 chemistry, Interleukin-8 metabolism, Intestinal Mucosa cytology, Lipopolysaccharides pharmacology, Peptides analysis, Peptides chemistry, Peptides metabolism, Proteome drug effects, Chemokines analysis, Chromatography, Liquid methods, Epithelial Cells metabolism, Intestinal Mucosa metabolism, Tandem Mass Spectrometry methods
- Abstract
Rationale: Intestinal epithelial cells (IEC) secrete many chemokines in response to proinflammatory stimuli. We investigated their role in the mucosal inflammatory response in the intestine, by developing a non-targeted approach for analyzing the profile of peptides secreted by stimulated IEC, based on differential mass spectrometry analysis., Methods: Lipopolysaccharide (LPS) was incubated with IEC as a proinflammatory stimulus. Differential peptidomic analysis was then carried out, comparing the profiles of IEC with and without LPS stimulation. A mass spectrometry procedure was developed, based on a liquid chromatography/tandem mass spectrometry (LC/MS/MS) approach without enzymatic pretreatment of the peptides. Partial de novo sequencing was carried out by Fourier transform ion cyclotron resonance (FTICR), and the native peptides in the culture media were identified., Results: A major ion (m/z 7862.51) detected after stimulation was identified as GRO alpha and a minor ion (m/z 8918.17) was identified as IL-8. ELISA-based comparisons gave results consistent with those obtained by MS. Surprisingly, GRO alpha was secreted in amounts 5 to 15 times higher than those for IL-8 in our cellular model. The truncated form of IL-8, resulting from activation, was detected and distinguished from the native peptide by MS, whereas this was not possible with enzyme-linked immunosorbent assay (ELISA)., Conclusions: Mass spectrometric analysis of culture media can be used to identify the principal peptides produced in response to the stimulation of IEC, and their metabolites. Mass spectrometry provides a comprehensive view of the chemokines and peptides potentially involved in gut inflammation, making it possible to identify the most appropriate peptides for further quantification., (Copyright © 2013 John Wiley & Sons, Ltd.)
- Published
- 2013
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21. [Intestinal microbiota: from antibiotic-associated diarrhea to inflammatory bowel diseases].
- Author
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Quévrain E and Seksik P
- Subjects
- Carbohydrate Metabolism physiology, Diarrhea microbiology, Humans, Inflammatory Bowel Diseases etiology, Intestinal Mucosa metabolism, Intestines drug effects, Metagenome drug effects, Obesity etiology, Obesity microbiology, Anti-Bacterial Agents adverse effects, Diarrhea chemically induced, Inflammatory Bowel Diseases microbiology, Intestines microbiology, Metagenome physiology
- Abstract
The intestinal microbiota is known to be composed by several hundred different bacterial species and is stable over time. Antibiotics intake induces a disturbance in the composition of intestinal microbiota which impairs its protective role against infection by pathogens. The presence of a large biomass of bacteria in the digestive tract exerts physiological effects with beneficial consequences for the host. Patients with inflammatory bowel diseases, exhibit an imbalance in the composition of intestinal microbiota called dysbiosis., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
22. Assessing calcareous sponges and their associated bacteria for the discovery of new bioactive natural products.
- Author
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Roué M, Quévrain E, Domart-Coulon I, and Bourguet-Kondracki ML
- Subjects
- Animals, Marine Biology, Molecular Structure, Aquatic Organisms chemistry, Bacteria chemistry, Biological Products chemistry, Biological Products isolation & purification, Biological Products pharmacology, Porifera microbiology
- Abstract
An overview of the chemistry and microbiology of calcareous sponges (Calcispongiae) is provided, highlighting the potential of these sessile filter-feeding marine invertebrates and their associated bacteria for the discovery of new bioactive natural products. 103 compounds are presented and 116 references cited.
- Published
- 2012
- Full Text
- View/download PDF
23. New 2-methyl-13-icosenoic acid from the temperate calcisponge Leuconia johnstoni.
- Author
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Quévrain E, Barnathan G, Meziane T, Domart-Coulon I, Rabesaotra V, and Bourguet-Kondracki ML
- Subjects
- Acetals analysis, Animals, Fatty Acids analysis, Fatty Acids, Unsaturated analysis, France, Gas Chromatography-Mass Spectrometry, Plasmalogens analysis, Aquatic Organisms chemistry, Fatty Acids, Monounsaturated analysis, Porifera chemistry
- Abstract
The fatty acid composition of the temperate calcareous marine sponge Leuconia johnstoni Carter 1871 (Calcaronea, Calcarea) was characterized for the first time in specimens collected off the Brittany coast of France over four years from October 2005 to September 2008. Forty-one fatty acids (FA) with chain lengths ranging from C₁₄ to C₂₂ were identified as fatty methyl esters (FAME) and N-acyl pyrrolidide (NAP) derivatives by gas chromatography-mass spectrometry (GC-MS). Twenty-two saturated fatty acids (SFA) were identified accounting for 52.1-59.0% of the total FA and dimethylacetals (DMA). In addition, among the SFA, we noticed the presence of numerous methyl-branched iso and anteiso FA, suggesting a large number of associated bacteria within L. johnstoni. Thirteen monounsaturated fatty acids (MUFA, 28.0-36.0% of total FA + DMA) were also identified as well as six polyunsaturated fatty acids (PUFA, 4.0-8.2%). A noticeable DMA was detected at a high level, particularly in September 2008 (11.8%), indicating the presence of plasmalogens in this sponge species. This calcareous sponge lacked the non-methylene-interrupted FA (NMI FA) with a Δ5,9 system typical of siliceous Demosponges and Hexactinellids. The occurrence of the unusual 8,13-octadecadienoic acid was reported for the first time as a minor PUFA in a calcareous sponge. The major FA, representing 20-25% of this sponge FA, was identified as the new 2-methyl-13-icosenoic acid from mass spectra of its methyl ester and its corresponding N-acyl pyrrolidide derivatives as well as a dimethyl disulfide adduct.
- Published
- 2012
- Full Text
- View/download PDF
24. Bioactive indole derivatives from the South Pacific marine sponges Rhopaloeides odorabile and Hyrtios sp.
- Author
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Longeon A, Copp BR, Quévrain E, Roué M, Kientz B, Cresteil T, Petek S, Debitus C, and Bourguet-Kondracki ML
- Subjects
- Animals, Antioxidants isolation & purification, Blood Proteins isolation & purification, Indoles chemistry, Indoles pharmacology, Magnetic Resonance Spectroscopy, Indoles isolation & purification, Porifera chemistry
- Abstract
Indole derivatives including bromoindoles have been isolated from the South Pacific marine sponges Rhopaloeides odorabile and Hyrtios sp. Their structures were established through analysis of mass spectra and 1D and 2D NMR spectroscopic data. Their potential inhibitory phospholipase A₂ (PLA₂), antioxidant and cytotoxic activities were evaluated. The new derivative 5,6-dibromo-L-hypaphorine (9) isolated from Hyrtios sp. revealed a weak bee venom PLA₂ inhibition (IC₅₀ 0.2 mM) and a significant antioxidant activity with an Oxygen Radical Absorbance Capacity (ORAC) value of 0.22. The sesquiterpene aureol (4), also isolated from Hyrtios sp., showed the most potent antioxidant activity with an ORAC value of 0.29.
- Published
- 2011
- Full Text
- View/download PDF
25. Novel natural parabens produced by a Microbulbifer bacterium in its calcareous sponge host Leuconia nivea.
- Author
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Quévrain E, Domart-Coulon I, Pernice M, and Bourguet-Kondracki ML
- Subjects
- Alteromonadaceae classification, Alteromonadaceae isolation & purification, Animals, Anti-Infective Agents metabolism, Base Sequence, Chromatography, Liquid, Molecular Sequence Data, Parabens metabolism, Parabens pharmacology, Phylogeny, Porifera chemistry, Staphylococcus aureus drug effects, Symbiosis, Alteromonadaceae metabolism, Anti-Infective Agents chemistry, Parabens chemistry, Porifera microbiology
- Abstract
A broad variety of natural parabens, including four novel structures and known ethyl and butyl parabens, were obtained from culture of a Microbulbifer sp. bacterial strain isolated from the temperate calcareous marine sponge Leuconia nivea (Grant 1826). Their structures were elucidated from spectral analysis, including mass spectrometry and 1D and 2D nuclear magnetic resonance. Their antimicrobial activity evaluated against Staphylococcus aureus was characterized by much higher in vitro activity of these natural paraben compounds 3-9 than commercial synthetic methyl and propyl parabens, usually used as antimicrobial preservatives. Compounds 4 and 9 revealed a bacteriostatic effect and compounds 6 and 7 appeared as bactericidal compounds. Major paraben compound 6 was also active against Gram positive Bacillus sp. and Planococcus sp. sponge isolates and was detected in whole sponge extracts during all seasons, showing its persistent in situ production within the sponge. Moreover, Microbulbifer sp. bacteria were visualized in the sponge body wall using fluorescence in situ hybridization with a probe specific to L4-n2 phylotypes. Co-detection in the sponge host of both paraben metabolites and Microbulbifer sp. L4-n2 indicates, for the first time, production of natural parabens in a sponge host, which may have an ecological role as chemical mediators.
- Published
- 2009
- Full Text
- View/download PDF
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