Search

Your search keyword '"Quadrifoglio, F"' showing total 296 results
Start Over Author "Quadrifoglio, F"
296 results on '"Quadrifoglio, F"'

21. The solution structure of DNA-free Pax-8 paired box domain accounts for redox regulation of transcriptional activity in the Pax protein family

Author

Codutti, L., van Ingen, H., Vascotto, C., Fogolari, F., Corazza, A., Tell, G., Quadrifoglio, F., Viglino, P., Boelens, R., Esposito, G., NMR-spectroscopie, Sub NMR Spectroscopy, Dep Scheikunde, and NMR Spectroscopy 1

Subjects
body regions, animal structures, embryonic structures
Abstract

Pax-8 is a transcription factor belonging to the PAX genes superfamily and its crucial role has been proven both in embryo and in the adult organism. Pax-8 activity is regulated via a redoxbased mechanism centered on the glutathionylation of specific cysteines in the N-terminal region (Cys45 and Cys57). These residues belong to a highly evolutionary conserved DNA binding site: the Paired Box (Prd) domain. Crystallographic protein-DNA complexes of the homologues Pax-6 and Pax-5 showed a bipartite Prd domain consisting of two helix-turn-helix (HTH) motifs separated by an extended linker region. Here, by means of nuclear magnetic resonance, we show for the first time that the HTH motifs are largely defined in the unbound Pax-8 Prd domain. Our findings contrast with previous induced fit models, in which Pax-8 is supposed to largely fold upon DNA binding. Importantly, our data provide the structural basis for the enhanced chemical reactivity of residues Cys45 and Cys57 and explain clinical missense mutations that are not obviously related to the DNA binding interface of the paired box domain. Finally, sequence conservation suggests that our findings could be a general feature of the Pax family transcription factors.

Published
2008

38. Jellyfish in the Mediterranean Sea

Author

ROTTINI SANDRINI L, STRAVISI F, AVIAN M, D'ANGELA D, DELLA LOGGIA, ROBERTO, DEL NEGRO P, GAVINELLI E, GIORGI R, KOKELJ F, LONGINELLI A, PICCINETTI C, PRINCI T, QUADRIFOGLIO F, SCARPA C, SCUKA M, TUBARO, AURELIA, ROTTINI SANDRINI, L, Stravisi, F, Avian, M, D'Angela, D, DELLA LOGGIA, Roberto, DEL NEGRO, P, Gavinelli, E, Giorgi, R, Kokelj, F, Longinelli, A, Piccinetti, C, Princi, T, Quadrifoglio, F, Scarpa, C, Scuka, M, and Tubaro, Aurelia

Published
1991

47. The solution structure of DNA-free Pax-8 paired box domain accounts for redox regulation of transcriptional activity in the Pax protein family

Author

NMR-spectroscopie, Sub NMR Spectroscopy, Dep Scheikunde, NMR Spectroscopy 1, Codutti, L., van Ingen, H., Vascotto, C., Fogolari, F., Corazza, A., Tell, G., Quadrifoglio, F., Viglino, P., Boelens, R., Esposito, G., NMR-spectroscopie, Sub NMR Spectroscopy, Dep Scheikunde, NMR Spectroscopy 1, Codutti, L., van Ingen, H., Vascotto, C., Fogolari, F., Corazza, A., Tell, G., Quadrifoglio, F., Viglino, P., Boelens, R., and Esposito, G.

Published
2008

Catalog

Books, media, physical & digital resources
See catalog results