De Freitas, Luis, Jimenez, Doris, Pimentel, Sherley, Mitaine-Offer, Anne-Claire, Pouységu, Laurent, Quideau, Stéphane, Paululat, Thomas, Gonzalez-Mujica, Freddy, Rojas, Luis B., Rodríguez, María, and Lacaille-Dubois, Marie-Aleth
Five previously undescribed triterpene saponins, billiosides A-E, and a known analogue, were isolated from the seeds of Billia rosea (Planch. & Linden) C. Ulloa & P. Jørg. Their structures were elucidated on the basis of extensive 1D and 2D NMR experiments ( 1 H, 13 C, DEPT, COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC) and mass spectrometry as (3β,21β,22α)-3-[(2- O -β-D-glucopyranosyl- O -[α-L-arabinopyranosyl-(1 → 4)]-β-D-glucopyranosyl)oxy]-21-[((2 E ,6 S )-2,6-dimethyl-6-hydroxyocta-2,7-dienoyl)oxy]-22-(acetyloxy)-24-hydroxyolean-12-en-28-oic acid, (3β,21β,22α)-3-[(2- O -β-D-galactopyranosyl-β-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O -α-L-arabinopyranosyl-(1 → 4)-β-D-glucopyranoside, (3β,21β,22α)-3-[(2- O -β-D-galactopyranosyl- O -[α-L-arabinopyranosyl-(1 → 4)]-β-D-xylopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O -β-D-glucopyranoside, (3β,21β,22α)-3-[(2- O -β-D-galactopyranosyl- O -[α-L-arabinopyranosyl-(1 → 4)]-β-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O -β-D-glucopyranoside, (3β,21β,22α)-3 - [(2- O -β-D-galactopyranosyl- O -[α-L-arabinopyranosyl-(1 → 4)]-β-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O -β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside, and dipteroside A. Billiosides B and C exhibited moderate effects when tested as hepatic glucose-6-phosphatase inhibitors and as glucose intestinal absorption inhibitors, using in situ rat intestinal segments. [ABSTRACT FROM AUTHOR]