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Your search keyword '"Réglier-Poupet, Hélène"' showing total 23 results
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23 results on '"Réglier-Poupet, Hélène"'

10. Proficiency Program for Real-Time PCR Diagnosis of Bordetella pertussis Infections in French Hospital Laboratories and at the French National Reference Center for Whooping Cough and other Bordetelloses

Author

Caro, Valérie, primary, Guiso, Nicole, additional, Alberti, Corinne, additional, Liguori, Sandrine, additional, Burucoa, Christophe, additional, Couetdic, Gérard, additional, Doucet-Populaire, Florence, additional, Ferroni, Agnès, additional, Papin-Gibaud, Sophie, additional, Grattard, Florence, additional, Réglier-Poupet, Hélène, additional, Raymond, Josette, additional, Soler, Catherine, additional, Bouchet, Sylvie, additional, Charreau, Sandrine, additional, Couzon, Brigitte, additional, Leymarie, Isabelle, additional, Tavares, Nicole, additional, Choux, Mathilde, additional, Bingen, Edouard, additional, and Bonacorsi, Stéphane, additional

Published
2009
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11. Invasive Group B Streptococcal Infections in Infants, France

Author

Poyart, Claire, primary, Réglier-Poupet, Hélène, additional, Tazi, Asmaa, additional, Billoët, Annick, additional, Dmytruk, Nicolas, additional, Bidet, Philippe, additional, Bingen, Edouard, additional, Raymond, Josette, additional, and Trieu-Cuot, Patrick, additional

Published
2008
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15. Rapid detection of the “highly virulent” group B streptococcus ST-17 clone

Author

Lamy, Marie-Cécile, primary, Dramsi, Shaynoor, additional, Billoët, Annick, additional, Réglier-Poupet, Hélène, additional, Tazi, Asmaa, additional, Raymond, Josette, additional, Guérin, François, additional, Couvé, Elisabeth, additional, Kunst, Frank, additional, Glaser, Philippe, additional, Trieu-Cuot, Patrick, additional, and Poyart, Claire, additional

Published
2006
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19. The Sortase SrtA of Listeria monocytogenesIs Involved in Processing of Internalin and in Virulence

Author

Garandeau, Caroline, Réglier-Poupet, Hélène, Dubail, Iharilalao, Beretti, Jean-Luc, Berche, Patrick, and Charbit, Alain

Abstract

ABSTRACTListeria monocytogenesis an intracellular gram-positive human pathogen that invades eucaryotic cells. Among the surface-exposed proteins playing a role in this invasive process, internalin belongs to the family of LPXTG proteins, which are known to be covalently linked to the bacterial cell wall in gram-positive bacteria. Recently, it has been shown in Staphylococcus aureusthat the covalent anchoring of protein A, a typical LPXTG protein, is due to a cysteine protease, named sortase, required for bacterial virulence. Here, we identified in silico from the genome of L. monocytogenesa gene, designated srtA, encoding a sortase homologue. The role of this previously unknown sortase was studied by constructing a sortase knockout mutant. Internalin was used as a reporter protein to study the effects of the srtAmutation on cell wall anchoring of this LPXTG protein in L. monocytogenes. We show that the srtAmutant (i) is affected in the display of internalin at the bacterial surface, (ii) is significantly less invasive in vitro, and (iii) is attenuated in its virulence in the mouse. These results demonstrate that srtAof L. monocytogenesacts as a sortase and plays a role in the pathogenicity.

Published
2002
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20. Ertapenem Resistance of Escherichia coli.

Author

Lartigue, Marie-Frédérique, Poirel, Laurent, Poyart, Claire, Réglier-Poupet, Hélène, and Nordmann, Patrice

Subjects
DRUG resistance, ESCHERICHIA coli, MEMBRANE proteins, BETA lactamases, PHARMACOLOGY
Abstract

An ertapenem-resistant Escherichia coli isolate was recovered from peritoneal fluid in a patient who had been treated with imipenem/cilastatin for 10 days. Ertapenem resistance may be explained by a defect in the outer membrane protein and production of extended-spectrum β-lactamase CTX-M-2. [ABSTRACT FROM AUTHOR]

Published
2007
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21. Comparative evaluation of Strepto B ID chromogenic medium and Granada media for the detection of Group B streptococcus from vaginal samples of pregnant women.

Author

Tazi A, Réglier-Poupet H, Dautezac F, Raymond J, and Poyart C

Subjects
Female, Humans, Pregnancy, Pregnant Women, Sensitivity and Specificity, Bacteriological Techniques methods, Carrier State diagnosis, Culture Media, Streptococcus agalactiae isolation & purification, Vagina microbiology
Abstract

Two types of selective media, the chromogenic medium Strepto B ID and two non-chromogenic media Strepto B agar and the Granada medium, were tested and compared to blood agar plates (BAP) for screening of Group B streptococcus vaginal colonization in pregnant women. All tested media were comparable in terms of sensitivity however, their use in routine laboratories may markedly facilitate the rapid detection of GBS in vaginal samples.

Published
2008
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22. Performance of chromID ESBL, a chromogenic medium for detection of Enterobacteriaceae producing extended-spectrum beta-lactamases.

Author

Réglier-Poupet H, Naas T, Carrer A, Cady A, Adam JM, Fortineau N, Poyart C, and Nordmann P

Subjects
Bacteriological Techniques, Enterobacteriaceae classification, Enterobacteriaceae genetics, Enterobacteriaceae growth & development, False Positive Reactions, Humans, Polymerase Chain Reaction methods, Predictive Value of Tests, Sensitivity and Specificity, beta-Lactam Resistance genetics, beta-Lactamases genetics, Chromogenic Compounds metabolism, Culture Media, Enterobacteriaceae enzymology, Enterobacteriaceae Infections microbiology, beta-Lactamases biosynthesis
Abstract

The chromogenic agar medium chromID ESBL (bioMérieux) was compared with BLSE agar medium (AES) for selective isolation and presumptive identification of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from clinical samples. A total of 765 samples (468 rectal swabs, 255 urine samples and 42 pulmonary aspirations) obtained from 547 patients was processed. All bacterial strains isolated on either medium were further characterized using biochemical tests, and ESBL producers were confirmed by synergy testing. Genetic characterization of ESBL genes was determined by PCR. A total of 33 ESBL-producing Enterobacteriaceae strains [Escherichia coli (n=16), Klebsiella pneumoniae (n=8), Enterobacter spp. (n=3), Citrobacter spp. (n=5) and Proteus mirabilis (n=1)] was recovered. The sensitivity after 24 h incubation was 88 % for chromID ESBL and 85 % for BLSE agar. At 48 h, the sensitivity of chromID ESBL increased to 94 % and was higher than that obtained with BLSE agar. The positive predictive value at 24 h for chromID ESBL was 38.7 % [95 % confidence interval (95 % CI) 28.3 -50.2 %)], which was significantly higher than that for BLSE agar [15.4 %, 95 % CI 10.1 -21.5 %]. On both media, false-positive results were mostly due to Pseudomonas aeruginosa and to Enterobacteriaceae overproducing chromosomal cephalosporinase (Enterobacter spp.) or a chromosomal penicillinase (Klebsiella oxytoca). This study showed that chromID ESBL, a ready-to-use chromogenic selective medium, is sensitive and specific for rapid, presumptive identification of ESBL-producing Enterobacteriaceae. Its chromogenic properties and its selectivity are particularly useful in specimens containing resident associated flora.

Published
2008
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