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7 results on '"Röllen, Katrin"'

1. Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins

Author

Röllen, Katrin, primary, Granzin, Joachim, additional, Remeeva, Alina, additional, Davari, Mehdi D., additional, Gensch, Thomas, additional, Nazarenko, Vera V., additional, Kovalev, Kirill, additional, Bogorodskiy, Andrey, additional, Borshchevskiy, Valentin, additional, Hemmer, Stefanie, additional, Schwaneberg, Ulrich, additional, Gordeliy, Valentin, additional, Jaeger, Karl-Erich, additional, Batra-Safferling, Renu, additional, Gushchin, Ivan, additional, and Krauss, Ulrich, additional

Published
2021
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2. The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins

Author

Röllen, Katrin, primary, Granzin, Joachim, additional, Remeeva, Alina, additional, Davari, Mehdi D., additional, Gensch, Thomas, additional, Nazarenko, Vera V., additional, Kovalev, Kirill, additional, Bogorodskiy, Andrey, additional, Borshchevskiy, Valentin, additional, Hemmer, Stefanie, additional, Schwaneberg, Ulrich, additional, Gordeliy, Valentin, additional, Jaeger, Karl-Erich, additional, Batra-Safferling, Renu, additional, Gushchin, Ivan, additional, and Krauss, Ulrich, additional

Published
2021
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3. Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein

Author

Röllen, Katrin, Granzin, Joachim, Batra-Safferling, Renu, and Stadler, Andreas Maximilian

Subjects
Photoreceptors, Sensory Receptors, Flavin Mononucleotide, Social Sciences, lcsh:Medicine, Crystallography, X-Ray, Photoreceptors, Microbial, Biochemistry, Protein Structure, Secondary, Small-Angle Scattering, Scattering, Spectrum Analysis Techniques, X-Ray Diffraction, Animal Cells, Relaxation Time, Psychology, Post-Translational Modification, lcsh:Science, Neurons, Crystallography, Flavin, Physics, Absorption Spectroscopy, Condensed Matter Physics, Chemistry, Physical Sciences, Crystal Structure, Sensory Perception, ddc:500, Cellular Types, Research Article, Signal Transduction, Chemical physics, Research and Analysis Methods, Bacterial Proteins, Protein Domains, Scattering, Small Angle, Solid State Physics, ddc:610, Relaxation (Physics), Flavoproteins, Pseudomonas putida, fungi, lcsh:R, Biology and Life Sciences, Proteins, Afferent Neurons, Dimers (Chemical physics), Cell Biology, Oxygen, Kinetics, Cellular Neuroscience, Ultraviolet-Visible Spectroscopy, Spectrophotometry, Ultraviolet, lcsh:Q, Neuroscience
Abstract

PLOS ONE 13(7), e0200746 - (2018). doi:10.1371/journal.pone.0200746, Light, oxygen, voltage (LOV) photoreceptors consist of conserved photo-responsive domains in bacteria, archaea, plants and fungi, and detect blue-light via a flavin cofactor. We investigated the blue-light induced conformational transition of the dimeric photoreceptor PpSB1-LOV-R66I from Pseudomonas putida in solution by using small-angle X-ray scattering (SAXS). SAXS experiments of the fully populated light- and dark-states under steady-state conditions revealed significant structural differences between the two states that are in agreement with the known structures determined by crystallography. We followed the transition from the light- to the dark-state by using SAXS measurements in real-time. A two-state model based on the light- and dark-state conformations could describe the measured time-course SAXS data with a relaxation time τREC of ~ 34 to 35 min being larger than the recovery time found with UV/vis spectroscopy. Unlike the flavin chromophore-based UV/vis method that is sensitive to the local chromophore environment in flavoproteins, SAXS-based assay depends on protein conformational changes and provides with an alternative to measure the recovery kinetics., Published by PLOS, San Francisco, California, US

Published
2018

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