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Like the early evolutionary theory (though unlike Darwin’s own output), much work in literary adaptation today operates only in terms of higher and lower forms, considering adaptations as more or less „faithful” to the „original”. In biology, it was only when this sort of evaluative discourse was discarded that new questions could be asked and therefore new answers offered. To that end, a biologist and a literary theorist work to develop the homology between biological and cultural adaptation, between natural and cultural selection: stories, in a manner parallel to genes, replicate; adaptations of both evolve with changing environments. Their „success” cannot and should not, in either case, be limited to their degree of „fidelity” to anything called a „source” or „original”.

BackgroundSeveral adverse events (AEs) occurring during immune checkpoint inhibitors (ICIs) therapy are clearly related to their mechanisms of action, and in this case they are indicated as immune-related AEs (irAEs). Every organ may be affected, including the musculoskeletal system; myositis, polymyalgia rheumatica, arthritis or arthritis have been reported in several retrospective and prospective case series and cohorts, with an incidence between 1.5% and 22%. While arthritis, vasculitis, myositis, and polymyalgia rheumatica are usually defined as “irAE” in RCTs, other rheumatic musculoskeletal conditions such as arthralgia, myalgia, back pain and muscular pain are often reported under the umbrella of “general” AEs.ObjectivesWe aimed to analyze rheumatic irAE and non-irAE due to immune-checkpoint inhibitors (ICIs) targeting programmed cell death-1 or its ligand PD-(L)1 in lung cancer patients from the available literature.MethodsWe performed a systematic review and meta-analysis of phase III randomized clinical trials (RCTs) assessing PD-(L)1 -ICIs in lung cancer patients, from inception until January 12th, 2021. We extracted data of each trial to estimate odds ratio (OR) for rheumatic ir or non-irAE as classified in RCTs safety data. Sensitivity analyses (by ICI, treatment group and histology) were performed.ResultsEighteen RCTs met the inclusion criteria (n=12172 subjects). The OR [95%IC] for rheumatic irAE in ICIs versus controls (either placebo or chemotherapy) was 2.20 [0.85,5.72].Among rheumatic non-irAEs, both overall (any grade, Figure 1A) and severe (grade≥3, Figure 1B) back pain were significantly more frequent in ICIs versus controls (2.01 [1.09;3.73] and 2.90 [1.18;7.08], respectively).Figure 1.Forest plot showing pooled odds ratio (OR) for back pain (5 phase III trials) (A) and severe back pain (4 phase III trials) (B), respectively.The overall frequency of arthralgia and severe arthralgia was similar between ICIs and controls (1.13 [0.86, 1.47] and 1.69 [0.68, 4.20], respectively). By sensitivity analysis RCTs assessing ICIs in combination with chemotherapy versus chemotherapy alone showed a significant association with arthralgia (1.55 [1.15;2.10]). Similarly, the frequency of myalgia did not differ between ICIs and controls, but was significantly lower in RCTs assessing ICIs alone versus chemotherapy (OR 0.32 [0.24;0.42]). Muscular pain was not significantly increased with ICI.ConclusionRheumatic irAEs are not increased in RCTs assessing PD-(L)1 inhibitors, not reflecting the real-life incidence, therefore likely underreported or misclassified. Back pain is significantly associated with PD-(L)1-ICIs regardless its severity, suggesting a possible implication of the PD-(L)1 axis in the development of inflammatory back pain in some patients.In addition, PD-(L)1-ICIs added on conventional chemotherapy are associated with a significantly higher frequency of arthralgia than ICI alone. This trend was seen in the other rheumatic AEs, suggesting that conventional chemotherapy might be a confounder in the interpretation of the occurrence of rheumatic AEs.Disclosure of InterestsAntonello Veccia: None declared, Marie Kostine: None declared, Alice Tison: None declared, Mariachiara Dipasquale: None declared, Stefania Kingspergher: None declared, Guido Grandi: None declared, Orazio Caffo: None declared, Sandro Inchiostro: None declared, Giuseppe Paolazzi: None declared, Roberto Bortolotti: None declared, Divi Cornec: None declared, Alvise Berti Consultant of: GSK

BackgroundThe ANCA-associated vasculitis patient-reported outcome (AAV-PRO) questionnaire is a 29-item disease-specific PRO measure for AAV[1]. The Italian version of the AAV-PRO questionnaire (AAV-PRO_ita) was translated in collaboration with Oxford and Bristol University (UK) and was preliminarily tested on a single-center Italian cohort[2].ObjectivesThe main objective of this multicentric study was to assess the internal consistency, feasibility, and reliability of the AAV-PRO_ita in a large cohort of Italian AAV patients. The secondary objective was to investigate the clinical characteristics of AAV patients associated with AAV-PRO_ita domains.MethodsThe AAV-PRO_ita is describes the following disease domains: (1) organ-specific and systemic symptoms and signs (SSS); (2) physical function (PF); (3) social and emotional impact (SEI). In this study, Italian-speaking AAV patients were recruited from Italian Centres (N=13) with a large experience in the diagnosis and treatment of systemic vasculitis, belonging to the Vasculitis Study Group of the Italian Society of Rheumatology. Inclusion criteria were: a confirmed diagnosis of GPA, MPA, or EGPA; ANCA positivity in at least once occasion or biopsy-proven AAV; and age ≥18 years old. Participants completed the AAV-PRO_ita at three different time-points: baseline, after 5-7 days, and at month 3.Results229 AAV-patients (56.3% women) with a median age of 61 (IQR 51-72) were recruited and completed the questionnaires. The subtype of AAV was mainly GPA (131, 57.2%), followed by EGPA (58, 25.3%), and MPA (40, 17.5%). Median BVASv3 at baseline was 0 (IQR 0-3), whereas the median BVASv3 at the onset of disease was 14 (IQR 9-20). Participants had a median duration of disease of 67 (IQR 24-126) months. Patients who experienced at least one relapse, one hospitalization, and one severe infection were 40.2%, 53.3%, and 24%, respectively. 83% of the patients were on immunosuppressant therapy and 71.6% were still receiving glucocorticoids (GC).AAV-PRO_ita questionnaire had good internal consistency (Cronbach’s Alpha range 0.81-0.93) and good test-retest reliability (ICCs range 0.93-0.96). Item response rates were high overall (maximum 0.87% missing data), supporting the feasibility of the questionnaire.Concerning the domains of the questionnaire, female AAV patients scored higher (i.e. worse) in all three domains, especially in the SEI domain (p-value0.3, p-valueConclusionThe AAV-PRO_ita questionnaire is a new 29-item, disease-specific PRO measure for use in AAV in the Italian language. It is a self-administered Italian questionnaire with good internal consistency, feasibility, and reliability. AAV-PRO_ita proved to be a useful tool to explore the AAV patient’s perception of quality of life, and it could become an important way of measuring the unmet needs of AAV patients.References[1]Robson, J.C. et al. Validation of the ANCA-Associated Vasculitis Patient-Reported Outcomes (AAV-PRO) Questionnaire. Ann. Rheum. Dis.2018, 77, 1157–1164, doi:10.1136/annrheumdis-2017-212713.[2]Treppo, E.; Palese, A.; Vita, S.D.; Quartuccio, L. AB0392 PRELIMINARY VALIDATION OF THE ITALIAN VERSION OF ANCA-ASSOCIATED VASCULITIS PATIENT-REPORTED OUTCOME (AAV-PRO_ita) QUESTIONNAIRE: FATIGUE AND CHRONIC PAIN AS UNMET NEEDS BY CURRENT TREATMENTS. Ann. Rheum. Dis.2021, 80, 1224–1224, doi:10.1136/annrheumdis-2021-eular.2123.AcknowledgementsWe thank the Italian Study Group on Systemic Vasculitis.Disclosure of InterestsNone declared

BackgroundRituximab (RTX) achieved high remission-induction and sustained maintenance rates for patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) [1], [2]. However, RTX is an expensive medication, which may potentially lead to serious side effects. Defining the best dose regimen for maintenance in AAV is still an unmet need.ObjectivesThe aim of the present study is to compare the effects of ultra-low dose RTX (500 mg or 1000 mg once per year) to standard low dose RTX (500 or 1000 mg twice per year) as remission-maintenance therapy in AAV patients.MethodsWe included consecutive AAV patients (classified as GPA and MPA [3]) referring to four different Rheumatology centers in Italy. We assessed all AAV patients who successfully achieved disease remission (BVASv3=0) with conventional RTX or cyclophosphamide regimens and have been subsequently treated with RTX for maintenance of remission. All included patients received at least three maintenance infusions with either 1000 mg or 500 mg, twice per year (standard low dose) or once per year (ultra-low dose). After a period of 18 months, we assessed the remission rate, damage (VDI), glucocorticoids intake, ANCA status, B-cells depletion and serum IgG levels.ResultsFrom January 2011 to December 2021, 83 AAV patients (mean age 51±16, 49.4% female, 95.2% ANCA positive, 65.8% anti PR3, 34.2% anti MPO), 61 classified as GPA and 22 MPA, achieved complete disease remission with conventional RTX induction regimen. After 7 [6-9] months, 29.9% patients started maintenance treatment with ultra-low dose RTX (once per year), while 70.1% patients with standard low dose (twice per year), for 18 months. No significant differences at baseline were noted between patients receiving ultra-low dose when compared to those treated with conventional low-dose.At the end of observation period, a disease flare was observed in 22.7% of the low-dose group, and 21.2% in those treated with the standard dose (p=0.881). Relapse-free survival was comparable between the two group (log-rank p=0.818, Figure 1).Figure 1.When comparing AAV patients treated with ultra-low dose regimen to those treated with low-dose, no differences were noted in negative ANCA rate (72.2% vs 67.1%, p=0.262), ANCA titer (0 [0-7.8] vs 0 [0-50] UI/mL, p=0.232), B-cells depletion rate (70.6% vs 75%, p=0.725), mean serum IgG (811 [146-922] vs 680 [429-861] mg/dL, p=0.367), mean daily glucocorticoid dosage (2.5 [0-5] vs 3.75 [0-5] mg/d, p=0.647), VDI (4 [1-5] vs 2 [1-4], p=0.098), hypogammaglobulinaemia rate (31.8% vs 36.5%, p=0.697) and deaths (4.5% vs 5.8%, p=0.831).Although not significant, patients treated with ultra-low dose had lower severe infection rate (10.5% vs 26.8%, p=0.154). Notably, in the all cohort 5 deaths were related to COVID19 pneumonia.ConclusionReduced exposure to RTX was not associated with an impaired efficacy of maintenance therapy in patients with AAV. Remission maintenance with ultra-low dose RTX is a safe and more cost-effective option.References[1]B. Terrier et al., “ANCA-associated vasculitides: Recommendations of the French Vasculitis Study Group on the use of immunosuppressants and biotherapies for remission induction and maintenance,” Press. Medicale, vol. 49, no. 3, 2020, doi: 10.1016/j.lpm.2020.104031.[2]S. V. Moiseev, N. M. Bulanov, A. S. Zykova, and P. I. Novikov, “Rituximab in ANCA-associated vasculitis: Fewer infusions or ultra low-dose maintenance therapy,” Ann. Rheum. Dis., vol. 78, no. 9, pp. 1–2, 2019, doi: 10.1136/annrheumdis-2018-213873.[3]J. C. Jennette et al., “2012 Revised International Chapel Hill consensus conference nomenclature of vasculitides,” Arthritis Rheum., vol. 65, no. 1, pp. 1–11, 2013, doi: 10.1002/art.37715.Disclosure of InterestsNone declared

Population limitation models of migratory birds have sought to include impacts from events across the full annual cycle. Previous work has shown that events occurring in winter result in some individuals transitioning to the breeding grounds earlier or in better physical condition than others, thereby affecting reproductive success (carry-over effects). However, evidence for carry-over effects from breeding to wintering grounds has been shown less often. We used feather corticosterone (CORTf) levels of the migratory Louisiana Waterthrush Parkesia motacilla as a measure of the physiological state of birds at the time of moult on the breeding territory to investigate whether carry-over effects provide linkages across the annual cycle of this stream-obligate bird. We show that birds arriving on wintering grounds with lower CORTf scores, indicating reduced energetic challenges or stressors at the time of moult, occupied higher quality territories, and that these birds then achieved a better body condition during the overwinter period. Body condition, in turn, was important in determining whether adult birds returned the following winter, with birds in better condition returning at higher rates. Together these data suggest a carry-over effect from the breeding grounds to the wintering grounds that is further extended with respect to annual return rates. Very few other studies have linked conditions during the previous breeding season with latent effects during the subsequent overwintering period or with annual survival. This study shows that the effects of variation in energetic challenges or stressors can potentially carry over from the natal stream and accumulate over more than one life-history period before being manifested in reduced survival. This is of particular relevance to models of population limitation in migratory birds.

Ecologists frequently use physiological tools to understand how organisms cope with their surroundings but rarely at macroecological scales. This study describes spatial variation in corticosterone (CORT) levels in feathers of invasive house sparrows (Passer domesticus) across their range in Mexico and evaluates CORT–climate relationships with a focus on temperature and precipitation. Samples were collected from 49 sites across Mexico. Feather CORT (CORTf) was measured using methanol-based extraction and radioimmunoassay. Relationships between CORTf and spatial and climate variables were examined using simple linear regressions. Ordination was used on climate data, CORTf was plotted against the resulting axes, and univariate regression trees were used to identify important predictors of CORTf. Universal kriging interpolation was used to illustrate spatial variation in CORTf across Mexico. Correlations with ordination axes showed that high CORTf was associated with low precipitation during the rainy season and low dry season temperatures. Specifically, CORTf was negatively related to May precipitation and January and July minimum temperatures, and positively related to April deuterium excess and June minimum temperatures. CORTf was higher in second-year birds compared to after-hatch years and after-second years. House sparrows had higher CORTf levels in the hot, dry, north-central region of Mexico, and CORTf was negatively related to temperature and precipitation. House sparrows molt primarily from August–September but climate conditions throughout the year were important predictors of CORTf, suggesting that conditions outside of molt can carry over to influence energetics during feather growth. These data suggest that dry conditions are challenging for house sparrows in Mexico, supporting previous work showing that precipitation is an important predictor of broad-scale CORT variation. This work highlights the utility of CORTf for evaluating the influence of physiology on current avian range limits; furthermore, these data may allow us to predict future changes in species distributions.

Reproductive success often declines with breeding date in diverse taxa, including temperate-nesting bird species. The date hypothesis predicts that seasonally deteriorating environmental quality drives this pattern. While mechanisms are not fully understood, a seasonal increase in parasitism may contribute to the declining quality of nestlings hatched later in the season. We examined the effect of ectoparasites on seasonal variation in indices of nestling quality and survival in Tree Swallows (Tachycineta bicolor (Vieillot, 1808)) at breeding sites in British Columbia (PG) and Saskatchewan (SDNRA). In a parasite removal experiment, we detected no seasonal trend in flea abundance and, contrary to expectation, there were fewer blow flies in nests of late breeders. Negative effects of parasites on nestlings were documented at PG, where lengths of primary feather and head–bill were affected. Parental, rather than environmental, quality had the greatest effect on reproductive success at PG, as nestling survival declined seasonally regardless of treatment and despite seasonally increasing food biomass. Nestlings in control (i.e., parasitized) nests at SDNRA had elevated feather corticosterone levels, but no other effects of parasites were detected possibly because parent birds were of higher quality or parents and offspring had greater access to food such that nestlings were capable of coping with parasite-related challenges.

Carotenoids produce many of the red, orange and yellow signal traits of birds, and individuals must trade off utilizing carotenoids for physiological processes versus ornamentation. Proximate mechanisms regulating this trade-off are poorly understood, despite their importance for expression of color signals. Corticosterone (CORT) may play a significant mechanistic role in signal expression because it mobilizes energy substrates and influences foraging behavior. We used a unique feather-based approach to test whether CORT mediates expression of carotenoid-based coloration. First, we investigated relationships between levels of CORT from feathers (CORT(f)) and carotenoid-based plumage signals in common redpolls (Acanthis flammea). Then, we determined how the width of growth bars and probability of having fault bars on feathers varied with CORT(f), specifically whether these metrics reflected developmental costs of elevated CORT ("stress" hypothesis) or represented an individual's quality ("quality" hypothesis). CORT(f) correlated positively with the strength of carotenoid signals, but only in adult males. However, also in adult males, CORT(f) was positively related to width of feather growth bars and negatively with probability of having fault bars, providing support for the quality hypothesis. Overall, CORT(f) was lower in adult males than in females or young males, possibly due to dominance patterns. Our results indicate that CORT may indirectly benefit feather quality, potentially by mediating the expression of carotenoid signals. We place our sex-specific findings into a novel framework that proposes that the influences of CORT in mediating carotenoid-based plumage traits will depend on the extent to which carotenoids are traded off between competing functions.

The study of population regulation is crucial for understanding population dynamics and for conservation. We report on trends in population size and reproduction of Bald Eagles (Haliaeetus leucocephalus) at Besnard Lake, Saskatchewan, Canada, during 1968-2012. We investigated the relative importance of density-dependent (population size) and density-independent (climate) factors in explaining variation in population growth rate and productivity. The number of occupied Bald Eagle territories increased until ca. 1988, but remained stable afterwards, fluctuating around ca. 26 pairs. The number of successful pairs increased until only ca. 1977 and remained relatively stable or slightly declined afterwards (ca. 16 successful breeding pairs per year). We found a strong negative density-dependence in all reproduction parameters (mean productivity, nesting success, mean brood size at fledging). Annual production initially increased in the 1970s, but decreased afterwards, while nesting success decreased throughout the whole study period. We also found a strong density-dependence in population growth rate, indicating that the stabilized population was regulated. It probably reached its carrying capacity in the late 1970s, although population size continued to increase until the late 1980s. Mean brood size at fledging was negatively related to the number of failed nesting pairs. Density alone explained most of the variation in breeding performance, although milder springs were weakly associated with a higher nesting success. Finally, we found evidence for regular fluctuations in mean productivity, and particularly in nesting success, with a 5-yr period. We discuss possible mechanisms behind the observed patterns of density-dependent reproduction and implications for our knowledge of how this eagle population is regulated.

Physiological mechanisms link the environment with population dynamics, and glucocorticoid hormones are of particular interest because they respond adaptively to environmental change and can influence vertebrate reproduction and fitness. We tested a novel approach of synchronizing feather-based measures of corticosterone (the primary avian glucocorticoid; CORTf) and ratios of stable isotopes (SIs) of C (δ13C) and N (δ15N) to provide information about environmental conditions and an integrated physiological response to those conditions over the same period of feather synthesis. Using a fragmented metapopulation of Dupont’s larks Chersophilus duponti, an endangered steppe songbird, we analyzed interrelationships among CORTf, δ13C, δ15N, and the physical environment, including measures of habitat loss and fragmentation. CORTf was not related to any habitat variable measured directly. However, we detected a significant spatial structure to CORTf values and food availability, with greater similarity in both at smaller spatial scales. Using SIs as proxies for the local environment, we found CORTf was negatively related to δ13C. Values of CORTf, δ13C, and the relationship between the two were likely driven by variation in agricultural land use surrounding lark habitat patches. Our feather-based approach revealed that individual physiology was sensitive to environmental conditions (e.g., an interaction of food availability and variation in habitat) at a local scale, but not patch or landscape scales. Combining CORTf and SIs may be a promising tool because it can provide individual-based information about habitat, physiology, and their relationship during the same time period.

Among European breeding birds, those wintering in the Sahel region have undergone a sustained and severe decline. Long-term data show that variation in primary production of the Sahelian staging area significantly affects survival of many species, a relationship probably mediated by trophic resource availability. However, the physiological, hormonal and behavioural responses underlying this relationship remain unexplored. We present a potential explanation for the importance of prevailing conditions during winter to understand the population ecology and current trends of migratory species. We measured corticosterone levels in feathers of Egyptian vultures Neophron percnopterus grown in Africa and Europe, showing how conditions faced by birds during wintering periods result in the release of more corticosterone over time than when those individuals were on their summering grounds. This pattern was concordant with home-range size differences (c. 33 times larger in Africa than in Europe). We suggest that as wintering habitat of Egyptian vultures in the Sahel region has degraded during recent times, food availability has also been reduced. An increase in corticosterone during winter with a consequent increase in locomotor activity, for example, food searching behaviour, may normally be adaptive. However, enlarging home ranges could be futile if conditions are not better in the dispersal area, and costs of the higher corticosterone level, including energy expenditure from enhanced activity, may pose a significant trade-off. These physiological responses may be characteristic of other European trans-Saharan migrant birds that have undergone significant population declines.

Summary. The screaming cowbird Molothrus rufoaxillaris is described as being sexually monochromatic, although it has a rufous axillary patch that shows inter-individual size variation. We investigated potential sex differences in patch size in 82 molecularly-sexed adults. All males exhibited large patches and 23 females had small patches; however, seven females lacked a patch and nine had large patches. Patch size was associated with sex and independent of body condition, location or date of sampling. We discuss several hypotheses that may explain the incidence of this male character in some females.

Colour polymorphism in vertebrates is usually under genetic control and may be associated with variation in physiological traits. The melanocortin 1 receptor (Mc1r) has been involved repeatedly in melanin-based pigmentation but it was thought to have few other physiological effects. However, recent pharmacological studies suggest that MC1R could regulate the aspects of immunity. We investigated whether variation at Mc1r underpins plumage colouration in the Eleonora’s falcon. We also examined whether nestlings of the different morphs differed in their inflammatory response induced by phytohemagglutinin (PHA). Variation in colouration was due to a deletion of four amino acids at the Mc1r gene. Cellular immune response was morph specific. In males, but not in females, dark nestling mounted a lower PHA response than pale ones. Although correlative, our results raise the neglected possibility that MC1R has pleiotropic effects, suggesting a potential role of immune capacity and pathogen pressure on the maintenance of colour polymorphism in this species.

8 pages, 3 figures, 1 table, Offspring of long-lived species should face costs of parental trade-offs that vary with overall energetic demands encountered by parents during breeding. If sex differences exist in how parents make the trade-off, sex-specific differences may exist in the contribution of each parent to those costs. Adaptations of offspring facing such costs are not well understood, but the hormone corticosterone probably plays a role. We manipulated breeding effort in Cory's shearwaters (Calonectris diomedea) to increase costs to offspring and used an integrated measure of corticosterone from chick feathers to investigate how experimental variation in parental investment influences offspring physiology. Average foraging trip duration and foraging efficiency (FE) of breeding pairs were not related to chick corticosterone, but sex biases in FE were. Adult male investment was more strongly related to chick corticosterone than was female investment. Importantly, we show for the first time suppression of adrenocortical activity in nestling Procellariiform seabirds, and explain how our results indicate an adaptive mechanism invoked by chicks facing increased costs of parental trade-offs, Funding for this work was provided by the Natural Sciences and Engineering Research Council of Canada and the Stuart and Mary Houston Professorship in Ornithology (to G.R.B.), and projects REN2002-01 164 and BOS2000-0569-CO2-01 from the Spanish Government. G.D.F. was supported by a University of Saskatchewan Dean’s Scholarship, a Ruby Larson Scholarship, a Malcolm A. Ramsay Memorial Award, and the Nature Saskatchewan Graduate Student Grant

Despite the fact that the ability of animals to avoid being consumed by predators is influenced by their behaviour, morphology and life history, very few studies have attempted to integrate prey responses across these adaptation types. Here, our goal was to address the link between life-history traits (size and growth trajectory) of tadpoles and behavioural responses to predators. Specifically, we wanted to determine whether information learned about predators was influenced by prey growth trajectory before and after learning. We manipulated the size/growth trajectory of tadpoles by raising them under different temperatures. Tadpoles raised on a slow-growth trajectory (under cold conditions) and taught to recognize a salamander subsequently showed stronger responses after 2 weeks than tadpoles that were raised on a fast-growth trajectory (under warm conditions). When we account for the effect of size (r (2)=0.22) on the responses of prey to predator cues, we find that the growth trajectory pre-learning but not post-learning influences the learned responses of the tadpoles. The differences in responses to predators may reflect differential memory associated with the predator. To our knowledge, this is the first study that has attempted to link life-history traits (size and growth rate) with learning of predators. In order to gain a comprehensive understanding of antipredator responses of prey animals, we call for additional integrative studies that examine prey anti-predator responses across adaptation types.

Plumage coloration, particularly when carotenoid-based, is important in social signaling in birds. Although feather color is a relatively stable trait, individuals may modify it with >cosmetic> substances such as preen oils. In addition, dirt accumulation may influence plumage coloration and further affect signal perception by receivers. Here, we analyze the separate potential effects of preen oils and soil accumulation on the reflectance properties of carotenoid-pigmented feathers across the visual range of most bird species, which includes the ultraviolet (UV). Using the yellow portion of tail feathers of Bohemian waxwings (Bombycilla garrulus), we performed two separate experiments where: (a) preen oils and/or soil were removed, or (b) preen oils (from black-billed magpies Pica pica or eagle owls Bubo bubo) were added. Preen oil addition reduced brightness but increased UV hue and yellow chroma. UV chroma was reduced by the addition of magpie (but not owl) preen oil. Soil accumulation had little effect on plumage reflectance in the UV range but significantly reduced yellow chroma. According to models of avian vision, both of these effects are detectable by birds and biologically meaningful when compared with natural variation between the sexes and age classes. We conclude that preen oil and soil accumulation can significantly affect the UV-visible reflectance of carotenoid-based plumages. As such traits typically advertise individual quality, preening and soiling have the potential to modify the information content of carotenoid-based plumage traits and how these signals are perceived by receivers., L. P.-R. was supported by a postdoctoral contract (07/028-A) from Junta de Comunidades de Castilla-La Mancha (JCCM) and a “Juan de la Cierva” contract from the Spanish Ministerio de Ciencia e Innovación (MICINN). Funding was also provided by Natural Sciences and Engineering Research Council of Canada and the Stuart and Mary Houston Professorship in Ornithology (to G. R. B).

For a comprehensive understanding of the evolution of animal signals, it is necessary to understand how the performance of visual displays is maximized to get the most possible attention from receivers. We assessed whether the white plumage of Snowy Owls Bubo scandiacus functioned as a social signal and, if so, how coloration and behavioural adaptations enhance signal efficacy. Signalling theory predicts that: (1) the colour properties of plumage should vary across the body, with the brightest parts being those involved in visual display performance; (2) specific displays calling attention to or enhancing detection or conspicuousness to conspecifics should be evident; and (3) location of the signallers should be such that signal efficacy is optimized. All three predictions were supported. The brightest areas of the plumage (particularly the face, throat and upper breast) were always unspotted, and white is particularly effective in open habitats characteristic of this species. The birds displayed a specific posture and orientated toward the sun preferentially on sunny days, and Owls with the whitest (least spotted) plumage displayed more and signalled more frequently from perches on the ground, where albedo from the snow may enhance the visual display. Snowy Owls integrate coloration, behaviour and environment through habitat selection to maximize the efficacy of their visual displays.

In the Athabasca oil sands region of northern Alberta, mining companies are evaluating reclamation using constructed wetlands for integration of tailings. From May to July 2008, reproductive performance of 40 breeding pairs of tree swallows (Tachycineta bicolor), plus growth and survival of nestlings, was measured on three reclaimed wetlands on two oil sands leases. A subset of nestlings was examined for i) feather corticosterone levels, ii) delayed-type hypersensitivity response, and iii) innate immune function. Nestlings on one of two wetlands created with oil sands process affected material (OSPM) were heavier and had greater wing-lengths, and mounted a stronger delayed-type hypersensitivity response compared those on the reference wetland. Corticosterone was significantly higher in male nestlings on one of two OSPM-containing wetland compared to the reference wetland. Body condition of 12-day-old female nestlings was inversely related to feather corticosterone. Under ideal weather conditions, reclaimed wetlands can support healthy populations of aerially-insectivorous birds.

Declines in American Kestrel (Falco sparverius) populations are widely reported, and Breeding Bird Survey (BBS) data suggest that the North American population declined significantly from 1984 to 2007. Potential causes include the spread of West Nile virus (WNV), increases in populations of Cooper's Hawks (Accipiter cooperii), and loss of suitable habitat. We examined trends in the numbers of both migratory and resident kestrel populations that use nest boxes in eight study areas in Florida, Georgia, Virginia and Maryland, New Jersey, Massachusetts, Pennsylvania, Saskatchewan, and the Yukon Territory, 1984–2007. All eight populations underwent significant declines; the mean annual decline in nest-box occupancy rate was 3.0% and ranged from 0.6% in Pennsylvania to 4.7% in New Jersey. Except for the most recent nest-box program, established in 1995 and declining since 2002, all nest-box populations began to experience declines before WNV arrived in North America in 1999. To test whether changes in ...

Food-supplementation studies on birds during egg laying have generally manipulated macronutrients and energy. Such studies have shown variable effects on egg size in American Coots (Fulica americana). We determined the relative influences of local environment, food quantity, and food quality on egg size in American Coots by supplementing food and carotenoids at three sites in Saskatchewan. Eggs were collected and analyzed for carotenoid content and stable isotopes (δ15N and δ13C) in the yolk to determine whether variation in the type of food eaten contributes to egg size. We also report on the isotopic analysis of American Coot tissues and their eggs to assess evidence of endogenous versus exogenous protein and lipid allocations to reproduction. We provide isotopic evidence that American Coots used endogenous lipid, but not protein, reserves for egg formation and that egg size is more dependent on exogenous sources of nutrients. Laying sequence was the only variable across nesting locales that h...

4 pages, 1 figure, 1 table.-- PMID: 19351566 [PubMed].-- Printed version published Jul 2009., Porphyrins are a widespread group of pigments in nature, but, contrary to melanins and carotenoids, their occurrence as plumage colorants seems to be anecdotal and their function, if any, is unknown. Using thin-layer chromatography and high pressure liquid chromatography, we have found coproporphyrin III, the same porphyrin type previously reported in owls, in the plumage of nestling black-shouldered kites (Elanus caeruleus). The first plumage grown at the nest in this species includes reddish-brown contour feathers in the upperparts, and particularly in the breast area, which fade during the weeks-long post-fledging period to become either gray or white consistent with the definitive adult plumage. In these reddish feathers, we have also found small amounts of pheomelanins and traces of eumelanin. The contribution of each pigment to the final colour perceived by birds or other animals is unknown. In white and grey feathers of the same species no porphyrin was found, and only traces of eumelanin were detected in the grey ones. The fact that the reddish feathers are only found in the juvenal plumage, when individuals are vulnerable in an open nest, leads us to hypothesize a camouflage role for this ephemeral plumage. As porphyrins are involved, although not exclusively, we can for the first time ascribe them a function in the plumage of birds., JJN and IMG were funded by project CGL2006-07481/BOS of the Spanish Ministry of Science and Education. GRB was funded by the Natural Sciences and Engineering Research Council of Canada and the Stuart and Mary Houston Professorship in Ornithology.

Responses of parent birds to changes in demands by their offspring have typically been investigated by manipulating brood size and testing for altered patterns of provisioning. We applied an alternative method to breeding pairs of American Kestrels (Falco sparverius) by modifying the duration of their nesting periods. Rather than focusing on how hard parents can work on any given day, this novel approach tests how long they can sustain the stress of provisioning and the associated behavioral consequences. We manipulated nesting periods by exchanging nearly fledged young at the age of 20 days with offspring from other nests that were 10 days old. We found that males provisioned their second foster broods at rates similar to those for their original broods. By contrast, females brooded offspring in second foster nests less and also delivered less food to them. As a consequence, male offspring in second nests gained less mass than those in first nests, and nestlings of both sexes had reduced feather...

Benzene and toluene are representative volatile organic compounds (VOC) released during production, storage, and transportation associated with the oil and gas industry and are chemicals of concern, as they are released in greater and possibly more biologically significant concentrations than other compounds. Most studies of air pollution in high oil and gas activity areas have neglected to consider risks to birds, including top-level predators. Birds can be used as highly sensitive monitors of air quality and since the avian respiratory tract is physiologically different from a rodent respiratory tract, effects of gases cannot be safely extrapolated from rodent studies. Wild and captive male American kestrels were exposed for approximately 1 h daily for 28 d to high (rodent lowest-observed-adverse-effect level [LOAEL] of 10 ppm and 80 ppm, respectively) or environmentally relevant (0.1 ppm and 0.8 ppm, respectively) levels of benzene and toluene. Altered immune responses characteristic of those seen in mammalian exposures were evident in kestrels. A decreased cell-mediated immunity, measured by delayed-type hypersensitivity testing, was evident in all exposed birds. There was no effect on humoral immunity. Plasma retinol levels as measured by high-performance liquid chromatography (HPLC) analysis were decreased in wild and captive kestrels exposed to the rodent LOAEL for combined benzene and toluene. This study indicates that American kestrels are sensitive to combined benzene and toluene. The study also illustrates the need for reference concentrations for airborne pollutants to be calculated, including sensitive endpoints specific to birds. Based on these findings, future studies need to include immune endpoints to determine the possible increased susceptibility of birds to inhaled toxicants.

1. Stress has pervasive consequences for the well-being of animals. Currently, understanding how individuals cope with stressors is typically accomplished via short-term quantification of blood glucocorticoids released after activation of the hypothalamic–pituitary–adrenal (HPA) axis. 2. We investigated whether the amount of corticosterone (CORT) deposited in growing feathers provides a long-term, integrated measure of HPA activity in birds using captive red-legged partridges Alectoris rufa as a model species. 3. We examined CORT levels in primary feathers induced to grow at the same time as stress series were performed with a capture and restraint protocol. Plasma CORT titres after stress-induced stimulation, but not baseline values, correlated with feather CORT. Feather levels showed the same pattern as plasma of decline across the breeding season, but more severely. 4. For females, CORT in naturally moulted flank feathers was highly and positively correlated with the number of eggs laid in the previous few months, but not clutch size of the following year. For males, the amount of black on a feather, known to be a social signal, was positively correlated with its CORT level. 5. The analysis of feather CORT is a novel methodology that allows for meaningful interpretations of how individuals respond to environmental perturbations and adjust to life-history stages. 6. The analysis of feather hormones has the unique advantages of allowing for experimentation and sampling at any time of the year with minimal investigator-induced impacts and artefacts, and shows the HPA activity of an individual with a flexible time frame from days to months depending on the length of time taken to grow the feather. As this technique can be applied to living or dead birds, or feathers picked up after moult, it provides the ultimate non-invasive physiological measure of considerable benefit in terms of animal welfare and sampling effort

Intraspecific variation in plumage may have a functional significance in mate choice. As a result, breeding birds are often paired assortatively with respect to colour. However, whether this within-pair correlation is a result of homotypic preference whereby individuals pick a mate that looks like themselves, or directional preference whereby all individuals have the same phenotypic preference, is often unknown. Using data collected between 1989 and 2006, we describe intraspecific variation in the striking white feathers on the leading edge of the wing, lesser coverts and mantle of 144 Spanish imperial eagles (Aquila adalberti), one of the most endangered birds of prey in the world. Females had, on average, more white than males, and pairs mated in a positive assortative fashion. Coloration was not related to age of the bird, food supply (i.e., territory quality) or breeding productivity. Our results are most consistent with the process of homotypic-preference assortative mating, and this may be a result of sexual imprinting, and function as a mechanism to optimize the degree of outbreeding. This pattern of mate selection may explain the rapid evolutionary divergence of A. adalberti from the continental population of imperial eagle A. heliaca., This study was funded by the Dirección General para la Biodiversidad del Ministerio de Medio Ambiente. GRB was supported by a grant from the Natural Sciences and Engineering Research Council of Canada.

It has been suggested that declines in breeding populations of Swainson's hawks (Buteo swainsoni) in California, Oregon, and Nevada may be due to differential mortality of hawks on their wintering grounds. Although massive mortality incidents reported on the wintering grounds partially support this suggestion, there are no data showing differential use of wintering areas by breeding populations of Swainson's hawks. We used stable-hydrogen isotope analysis of feathers to determine whether large flocks of hawks wintering in Argentina consisted of a mixture of individuals from across the North American breeding range or consisted of individuals from discrete breeding populations. We found that flocks of wintering Swainson's hawks consisted of a mixture of individuals. The lack of connectivity between populations of breeding and wintering hawks suggests that high wintering mortality, either natural or human-induced, is unlikely to have direct consequences on a single breeding area in North America. The demographic effects of winter mortality should be 'diluted' across the entire breeding range of Swainson's hawks.

Several species of raptors have two ocelli (eye-like patches) in the back of their head, giving them the appearance of a false face, although this trait has rarely been reported. According to our observations, these markings may be widespread in the family Falconidae, some Accipiter hawks, and some owls (e.g. Glaucidium, and some Athene, Aegolius, and Surnia). In this study, we outline general classes of hypotheses that may account for ocelli on the heads of raptors. The most frequently evoked general hypothesis is that ocelli offer protection against attackers. For example, a predator may abort the effort, be deflected by the ocelli, or be warned that the bearer will retaliate if attacked. We propose two alternatives. Most raptors with ocelli typically include a large proportion of passerine birds in their diets. The false faces may have evolved to aid in the hunting of small birds by actually provoking, or manipulating the nature of, a mobbing response. The benefit of doing so may either be immediate because there are numerous accounts of mobbers being killed, or the benefit may be postponed if the predator is using mobbing as a means of evaluating hunting prospects in a given area. An analysis of the pygmy-owls of the genus Glaucidium indicates that species displaying ocelli in the nape tend to have a high proportion of small birds in their diets and live in open habitats, whereas the opposite is true for species without ocelli. Pygmy-owls with ocelli are also considerably smaller and, collectively, these findings are most consistent with false faces being a conspicuous visual signal to deceive mobbing birds so they can be preyed upon. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society, 2007, 90, 467–477.

and journalistic discourse on the topic of narrative adaptation, the "orchid thief in Spike Jonze's film would like us to believe that adaptation is, in fact, a "profound process." In the immediate context, he means biological adaptation, of course, but in a metacinematic film about the process of adapting a book to the screen, the cultural implica tions of his positive remark should not be dismissed, despite its evident irony.1 The manifest ubiquity of narrative adaptations in contemporary culture notwithstanding, the critical tendency has been to denigrate them as secondary and derivative in relation to what is usually (and tellingly) referred to as the "original." Adaptation theory has rarely challenged this dismissive evaluation. Despite the theoretical sophistication of recent literary critical discourse, adaptation studies have remained stubbornly rooted in often unexamined values and practices. Although it seems self-evident that the insights of such theories as Bakhtinian dialogism, intertextuality, deconstruction, reception theory, cultural studies, narra tology, or performance theory might have relevance to adaptation stud ies, these connections have only begun to be made.2 In a way, therefore

In 1998, the Aznalco´ llar mine tailings dyke in southwestern Spain broke, flooding the Agrio-Guadiamar river system with acid tailings up to the borders of one of the largest breeding colonies of white storks in the western Palearctic, Dehesa de Abajo. Over the following years, a high proportion of nestlings developed leg defects not seen before the spill, prompting this study. Nestlings with deformed legs had significantly lower plasma phosphorous (P) and higher Ca:P ratios than non-deformed cohorts in the first two years, but in the third year, when more, younger birds were studied, plasma P ranged from much higher to much lower in the affected colony compared with reference birds. Coefficients of variation for phosphorous were 19% and 60%, in reference and contaminated colonies, respectively. Storks from the contaminated colony were unable to control P levels and Ca:P ratios within the narrow limits necessary for normal bone development.

Urban endocrine ecology aims to understand how organisms cope with new sources of stress and maintain allostatic load to thrive in an increasingly urbanized world. Recent research efforts have yielded controversial results based on short-term measures of stress, without exploring its fitness effects. We measured feather corticosterone (CORTf, reflecting the duration and amplitude of glucocorticoid secretion over several weeks) and subsequent annual survival in urban and rural burrowing owls. This species shows high individual consistency in fear of humans (i.e., flight initiation distance, FID), allowing us to hypothesize that individuals distribute among habitats according to their tolerance to human disturbance. FIDs were shorter in urban than in rural birds, but CORTf levels did not differ, nor were correlated to FIDs. Survival was twice as high in urban as in rural birds and links with CORTf varied between habitats: while a quadratic relationship supports stabilizing selection in urban birds, high predation rates may have masked CORTf-survival relationship in rural ones. These results evidence that urban life does not constitute an additional source of stress for urban individuals, as shown by their near identical CORTf values compared with rural conspecifics supporting the non-random distribution of individuals among habitats according to their behavioural phenotypes

Among the most familiar sexual signals are red, yellow, and orange sexual traits pigmented by carotenoids. Many birds can detect near-ultraviolet (UV) light, and UV signals can play key roles in mate choice. Grouse (Tetraonidae) exhibit bright carotenoid-dependent sexual ornaments, their supra-orbital combs, which to humans appear orange-red. Combs also reflect in the UV, which is not visible to humans but is likely to be visible to grouse. In male red grouse Lagopus lagopus scoticus, we show that comb UV reflectance decreases with increasing comb size and redness. By removing the epidermis of combs, where carotenoid pigments are, we show that the UV reflectance is a property of the dermis, underneath the red pigmented epidermis. Carotenoid pigmentation of combs acted as a mask to reduce reflectance by the dermis in the range 400–550 nm and in the UV, 300–400 nm. Patagium skin (non-ornamental skin under the wing) also reflects in the UV, but epidermis removal on this bare part tended to reduce UV reflectance, whereas removal of the red epidermis of combs increased UV reflectance. Males in better condition (greater body mass relative to size) had bigger and redder combs, but with less UV. Thus, carotenoid pigments of grouse combs are deposited on a white background with significant UV reflectance, which can influence how the signal is perceived by conspecifics. Carotenoid-based traits exhibit UV reflectance in a number of species, but how UV reflectance and carotenoid pigmentation influence colour remains little known for integumentary ornaments compared to plumage traits. UV vision is not uncommon in birds and other animals, so future studies should investigate how UV reflectance influences the perception of carotenoid-based signals of quality., F. Mougeot was supported by a Ramon y Cajal grant (Ministerio de Educación y Ciencia, Spain). L. Pérez-Rodríguez was supported by a pre-doctoral FPU grant (Ministerio de Educación y Ciencia). J. Martínez-Padilla was supported by a post-doctoral grant (ref. EX-27-04-04, Ministerio de Educación y Ciencia). G. Bortolotti was supported by a NSERC grant.

Although birds are highly patterned animals, the efficacy and information content of complex colour signalling by combining multiple feathers has rarely been investigated. We examine a melanin-dependent plumage pattern in the red-legged partridge, Alectoris rufa , in a captive breeding facility in Spain. Both males and females are striking in that the majority of the entire side of the body is covered with a bold brown, black and white vertical pattern. During agonistic displays the feathers are aligned in nearly perfect, continuous dorsoventral ‘flank bands’. We report on the unusual morphology and developmental complexity of the pattern, including the number and anatomical location of the display feathers. We investigate whether the area of any of the three colours of individual feathers was a signal of a bird’s quality. Black alone varied significantly with age, sex and mass scaled to body size in both sexes, as well as the heterophil/ lymphocyte ratio of females. Birds in poor condition appear not to be able to pigment the feather completely to its edge. The negative correlation between the black areas of the display feathers of mated pairs and the negative correlation between male ornament size and reproductive success suggest various behavioural associations with colour. Plumage patterns provide many more potential opportunities for signalling an individual’s quality than overall colour alone, and they have the advantage that they can be turned off and on at the appropriate time through behavioural modification.

Background/Objective Endocrine parameters have proven useful in the detection of early or low-level responses to pollutants. Although most of the studies on endocrine modulation have been focused on processes involving gonadal steroids, contaminants may target other parts of the endocrine system as well. In this study we examined the adrenocortical stress response and thyroid hormone status in free-living nestling white storks (Ciconia ciconia) in relation to heavy metals (zinc, lead, copper, cadmium) and arsenic levels in blood. Methods Fieldwork was conducted in an area polluted by the Aznalcóllar mine accident (southwestern Spain) and in a reference site. We used a standardized capture, handling, and restraint protocol to determine both baseline and maximum plasma corticosterone. Circulating levels of thyroxine (T4) and triiodothyronine (T3) were also measured. Results No effects of metals or As were found on baseline corticosterone, but maximum levels of corticosterone were positively related to Pb in both locations. This relationship was stronger in single nestlings than in birds from multiple-chick broods, which suggests a greater impact of Pb on more stressed individuals. Metal pollution did not affect plasma T4 or T3 levels, although thyroid status differed with location. Conclusions Because a compromised hypothalamus–pituitary–adrenal (HPA) function can have far-reaching consequences in terms of altered behavioral and metabolic processes necessary for survival, our results suggest that birds exposed to sublethal Pb levels may be at risk through an altered adrenocortical stress response, and further support the idea that HPA axis-related end points might be useful indicators of metal exposure and potential toxicity in wild animals.

There is currently a great deal of interest in documenting habitat use of birds in the boreal forest, especially in response to disturbances such as harvesting and forest fires. Studies have shown that some avian species are much more common in recently burned areas, whereas others are absent. Despite this, we know little about the immediate effect of a large forest fire, and its considerable alteration of habitat, on birds during a single breeding season. In 1995, a 72,000-ha forest fire in our study area gave us the opportunity to assess the effects of fire on reproduction of American kestrels (Falco sparverius) in comparison to an adjacent control area. In addition, we also examined territory occupancy by kestrels in the burned and control areas 8 y following the fire. We found no immediate effect of the fire on body condition of adult kestrels, their ability to hatch eggs, or to fledge at least one young. Birds breeding in the area that burned, however, did fledge fewer offspring per nest, and these nestlings had reduced body mass compared to control nests, possibly because of an immediate reduction in available prey following the fire. Nonetheless, given the profound alteration of habitat following the fire, the fact than any birds remained to continue breeding is remarkable. Territory occupancy of the burned area in 2003 was similar to 1995, the year of the fire, but occupancy in the adjacent control area declined during this period. It therefore appears that the control area is becoming a less suitable habitat for kestrels as the forest continues to mature, whereas the burned area is still providing viable habitat for kestrels. We present evidence suggesting that some of the differences in occupancy between the control and burned area may be due to fewer nest predators in the burned area in 2003.

We investigated whether polychlorinated biphenyl (PCB) exposure in American kestrels (Falco sparverius) influenced incubation behavior and whether altered behavior could lead to poor reproductive success. Captive kestrels were fed a mixture of PCBs (Aroclors 1248:1254:1260) at an approximate daily dose of 7 mg/kg body weight, 1 month prior to pairing and throughout incubation. Behaviors of 23 control and 23 PCB-exposed pairs were monitored throughout incubation using an electronic balance in the nest box. PCB exposure resulted in longer incubation periods and in altered incubation behaviors. Seven of 14 behavioral variables showed some association with treatment, with sex-specific effects largely biased toward disrupted male behavior. For most behaviors, the treatment effect was explained by the delayed clutch initiation induced by PCBs rather than by a direct physiological impact of the contaminants. PCB-exposed pairs with greater attendance to their eggs and better coordination of incubation duties had improved hatching success.

1. Food web interactions in animal communities can be investigated through the measurement of stable isotopes (e.g. δ15N, δ13C). We used this approach in a community of 14 species of seabirds breeding on the Argentinean Patagonian coast. Tissue samples were collected from nestling and adult seabirds, as well as prey, during three consecutive breeding seasons in 28 breeding colonies. 2. Relative to those in other temperate and polar regions, this seabird community showed a high degree of overlap in trophic level (TL) among species (93% of species within a TL range of 0.7) and also a comparatively high mean trophic level (4.1). 3. Relative positions of seabirds in relation to prey suggest that most species feed on pelagic fish and to a lesser extent on invertebrates. Stable isotope values of specialist feeders, Olrolg's (Larus atlanticus) and dolphin gulls (Leucophaeus scoresbii), which were previously assumed to feed mainly on crabs and sea lion excrement, respectively, suggested a broader diet than expected. 4. Based on stable isotope values of individuals, groups of phylogenetically related species generally showed a high degree of overlap within each group. 5. Given the degree of isotope overlap in this species-rich community, coexistence could be interpreted as a consequence of superabundance of food or species diversification in morphology and foraging strategies. The short range of trophic level makes these seabirds vulnerable to the reduction of fish stocks due either to commercial fishing or stochastic fluctuations.

SUMMARY Anti-actin IgA antibodies have been found in sera of coeliacs. Our aim was to define the prevalence and clinical significance of anti-actin IgA in coeliacs before and after gluten withdrawal. One hundred and two biopsy-proven coeliacs, 95 disease controls and 50 blood donors were studied. Anti-actin IgA were evaluated by different methods: (a) antimicrofilament positivity on HEp-2 cells and on cultured fibroblasts by immunofluorescence; (b) anti-actin positivity by enzyme-linked immuosorbent assay (ELISA); and (c) presence of the tubular/glomerular pattern of anti-smooth muscle antibodies on rat kidney sections by immunofluorescence. Antimicrofilament IgA were present in 27% of coeliacs and in none of the controls. Antimicrofilament antibodies were found in 25 of 54 (46%) coeliacs with severe villous atrophy and in three of 48 (6%) with mild damage (P

Summary 1. Bright coloration in birds is typically a sexually selected trait. Expression of such traits is sensitive to environmental factors, so they can function as bioindicators of environmental contamination. Of particular value may be carotenoid-based coloration because it is commonly used as a social signal and these pigments have important health functions. 2. American Kestrels (Falco sparverius) in captivity were exposed to dietary PCBs in March. Colour and plasma carotenoids of exposed and control birds were evaluated at pairing and courtship in April, and in winter in December. Juveniles produced by these birds (exposed to PCBs only in ovo) were examined at fledging and in winter. 3. The brightly coloured ceres and lores were evaluated by comparison to colour charts and quantified using digital photographs, and plasma carotenoid concentrations were quantified by spectrophotometry. 4. During breeding, PCB-exposed kestrels differed from controls for both colour and carotenoids, although the nature of effects was sex-specific. Carotenoids of juveniles were not related to treatment at fledging. 5. In winter, PCB-exposure resulted in patterns of colour/carotenoid variation opposite to controls; exposed adult males were duller, and juveniles of both sexes were brighter, than controls. PCB juveniles had higher plasma levels of carotenoids. Sexual dimorphism was apparent in colour and carotenoids of control adults, but not for PCB-exposed birds. 6. Our results are consistent with endocrine disruption. Modulation of both colour and carotenoids may have serious consequences to social behaviour and health.

Brood size has the potential to determine the allocation of resources between parents and offspring, as well as influence the relative contributions of each sex to parental effort. However, it is unclear whether brood size is the proximate determinant of parental effort, or conversely whether parental effort is the proximate factor to which brood size is adjusted. If brood size determines parental effort, then theory suggests that parental effort should vary with experimental changes in brood size. In contrast, if parental effort determines brood size, then parental effort is expected to be independent of experimental variation in brood size. To distinguish between these hypotheses, we experimentally reduced brood sizes of American kestrels (Falco sparverius). Our results suggest that male parents responded to brood-size variation and adjusted their provisioning behaviour accordingly. Conversely, female parents did not adjust provisioning in response to brood size, and as a result, offspring in reduced broods received more food on a per-nestling basis. However, condition and survival of offspring were similar in reduced broods and control young, which may have been the result of larger food requirements of small broods, owing to increased thermoregulatory costs compared with control broods. Female parents with reduced broods also did not brood offspring more often, further suggesting that females do not respond to variation in brood size. We conclude that the proximate determinants of parental effort are sex-specific in American kestrels: for males, brood size determines behaviour, whereas for females, behaviour may be a proximate factor determining brood size.

We determined that in ovo exposure to polychlorinated biphenyls (PCBs) alters growth off first-generation nestlings during and one year after parental exposure. Captive American kestrels (Falco sparverius) laid eggs with environmentally relevant total PCB levels (34.1 μg/g whole-egg wet wt) when fed PCB-spiked (Aroclor® 1248, 1254, and 1260) food (7 mg/kg body wt/d) for 100 d in 1998. In 1999, the same adults laid eggs with estimated total PCBs of 29.0 μg/g. Nonsurviving PCB-exposed chicks were small (mass, bones) in 1998. Survivors showed a strong sex-specific growth response (mass, bones) compared to respective sex controls: Only female hatchlings were larger, and only male nestlings had longer feathers (1998); maximal growth and bone growth rates also differed (males were advanced, faster; females delayed, slower) (1999); and male nestlings fledged earlier and were smaller, while females were larger (1998, 1999). However, regardless of sex, PCB-exposed nestlings generally grew at faster rates in both years. In 1998, greater contaminant burdens and toxic equivalent concentrations in sibling eggs were associated with nestlings being lighter, having longer bones and feathers, and growing at faster rates (mass, bone) for females but slower rates (mass) for males. Both physiological-biochemical and behavioral changes are likely mechanisms. This study supports and expands on the Great Lakes embryo mortality, edema, and deformities syndrome: While PCB exposure alters nestling size, maximal growth and growth rates also change immediately, are sustained, and are sex specific.

For most species of birds, little is known about the pattern and significance of intraspecific variation in iris colour. In early winter, captive American kestrels could be subjectively placed into at least two age categories: 0.5-yr-old birds had all-brown irides, whereas those of older birds were red-brown. As part of a toxicological study on kestrels, we quantified iris colour objectively using a digital camera to examine potential variation due to age, sex, and exposure to polychlorinated biphenyls (PCBs). Red, green, and blue values, plus an overall measure of colour using principal components analysis (PC1), were derived for breeding and nonbreeding kestrels 1.5-6.5 yr old (fed PCBs), plus offspring 0.5 yr old (exposed to PCBs only in ovo). Age category (0.5, 1.5, and 2.5+ yr) and PCB exposure consistently had an effect on colour, while sex was significant only for red and almost so for PC1. ANOVA with age as a covariate revealed that the amount of red continued to increase throughout life, but PCBs suppressed the development of that colour.

We experimentally examined the effects of dietary exposure to polychlorinated biphenyls (PCBs) on adrenocortical function in American kestrels (Falco sparverius). Nine captive male American kestrels previously exposed to a PCB mixture (Aroclor™1248:1254:1260; 1:1:1) in their diet were subjected to a standardized capture, handling and restraint protocol designed to produce an increase in circulating corticosterone. A similar protocol has been applied to a wide range of avian species and was used here to evaluate the response of PCB-exposed and control kestrels to a defined physical stressor. Both baseline and stress-induced corticosterone levels were significantly lower in PCB-exposed birds when compared with control birds of the same age. PCB-exposed birds exhibited significantly lower corticosterone levels during the corticosterone response when compared with control birds, independent of body condition. Furthermore, baseline corticosterone concentrations exhibited a hormetic response characterized by an inverted U-shaped dose response in relation to total PCB liver burden. These results support several recent studies which report decreased levels of circulating corticosterone in PCB-exposed wild birds. The results presented here provide the first evidence that exposure to an environmentally relevant level of PCBs (approximately 10 mg/kg body weight) can impair the corticosterone stress response in kestrels, potentially increasing the susceptibility of birds to environmental stressors such as severe weather and predatory and human disturbance.

Exposure to environmental contaminants can induce physiological changes in animals through various mechanisms. One manifestation of subclinical toxicity from polychlorinated biphenyl (PCB) exposure is the disruption of normal immune function described in numerous species, including American kestrels (Falco sparverius). In 1998, 152 mature male and female kestrels were fed either a mixture of Aroclor 1248:1254:1260 (approximately 7 mg/kg kestrel/day) through their food items, or control diets. Offspring produced by 50 breeding pairs (thus, half received in ovo PCB exposure only) were also studied. Total and differential white blood cell counts, the phytohemagglutinin (PHA) skin response, as well as thyroid hormone levels were tested in vivo in nonbreeding adults (1998 only) and nestlings (1998 and 1999). In 1999, nestlings came from three parental groups; adults exposed in 1998, birds produced by PCB-exposed parents, and unexposed birds. In 1998, directly exposed males but not females had increased total white blood cell counts driven by lymphocytosis, plus a decreased heterophil-to-lymphocyte ratio relative to controls. PCB-exposed birds had a significantly greater response to PHA than did controls, with sex as a significant factor and plasma triiodothyonine (T(3)) as a significant covariate. Levels of T(3) were significantly depressed in PCB-exposed birds of both sexes. The 1999 nestlings (F1 generation with respect to PCB exposure) did not show any effect of parental treatment group on the PHA skin response, yet T(3) remained as a significant covariate. Immunological effects are discussed in light of the antibody-mediated immunotoxicity found in the same birds and reported previously.

We studied variation in the carotenoid color of flight feathers of hybrid Northern Flickers (Colaptes auratus) and its correlation with reproductive performance and survival. Color scores provided by a digital camera revealed in 218 individuals a continuous spectrum from yellow to red. Males tended to be slightly redder than females. Within individuals, an analysis of color change with age revealed that males, but not females, became redder with age. Except for yearling females, body condition did not improve with age, sug- gesting that color is not linked to body condition measured during incubation. We did not detect any correlations between feather color and measures of reproductive performance, such as clutch size, hatching success, or fledging success, or return rate to the study area. In a hybrid population where intraspecific variation in color is controlled partly by genes, hue or brightness may not be a useful signal of individual quality, contrary to other studies of birds. About 25% of flickers had one or more tail feathers that differed from the rest of the plumage. In each case, the ''odd'' feathers were paler or yellower in color and may have been caused by diet or stress when the feathers were lost and regrown during winter. Such odd colors support the hypothesis that red carot- enoid pigments are costly to maintain under stressful conditions. Received 25 January 2002, accepted 5 August 2002.

The fatty acid compositions of yolk lipids of eggs collected in the wild from five species of ducks (Anatidae) showing a range of dietary preferences, the king eider Somateria spectabilis, the lesser scaup Aythya affinis, the mallard Anas platyrhynchos, the green-winged teal Anas crecca and the gadwall Anas streperi, are reported. For all five species, the fatty acid profiles conformed to a similar pattern, characterized by relatively balanced proportions (w/w) of arachidonic (6.7‐9.1%) and docosahexaenoic (5.9‐7.0%) acids in yolk phospholipid. This contrasts markedly with the yolk phospholipid of current commercially-reared ducks where the proportion of arachidonic acid can be 10 times greater than that of docosahexaenoic acid. It was most notable that the similarities in yolk fatty acid profiles among the different species of wild ducks were achieved despite the considerable interspecies variation in dietary modes. It seems that, in this instance, genetic factors deriving from a common phylogeny may be more important than dietary differences in determining the fatty acid composition of yolk.

For birds with altricial young, the brood-rearing period is one of the most energetically expensive periods in their lives and may be the bottleneck for fitness. Because parents are expected to be prudent in allocating resources between reproduction and self-maintenance, food supply should be an important factor determining reproductive decisions during brood rearing. Parents with abundant food are expected to have enhanced fitness because they are able to reduce their work rates and increase their own survival chances, because their offspring may be of higher quality, or some combination of these. However, few studies have simultaneously documented all of these variables in a single investigation. We performed a food supplementation experiment to test how food supply influenced provisioning decisions by parent American kestrels (Falco sparverius). Female kestrels showed a strong response to extra food and reduced their provisioning rates. As a result, supplemented females had higher return rates than control females, suggesting significant effects of food on female survival. Because females used extra food to increase their fitness, our results suggest that kestrels raising offspring are limited by food. Male kestrels whose nests were supplemented also responded to extra food by reducing provisioning, but to a much lesser extent than their mates. Male parents did not appear to benefit from supplementation, as their return rates were similar to control males. The total amount of food received on a daily basis by nestlings was similar between supplemented and control nests. Supplemented offspring therefore did not fledge in better condition or have higher survival rates than control nestlings; the only significant factor consistently affecting offspring condition and survival was weather.

Summary 1. Measures of the quality of an individual are important in the study of proximate and ultimate factors in biology. Records of developmental history are particularly desirable, as many phenotypical traits are influenced by conditions during growth. Conspicuous irregularities in feathers, known as fault bars, result from a variety of stresses that occur during feather growth. The frequency of fault bars was evaluated on primary and tail feathers (grown 1 year previously) of 1919 adult American kestrels from a breeding population in Canada (1990‐97). 2. Most (91·5%) birds exhibited some fault-barring, although females had significantly more feathers with fault bars than males (17% vs. 14%, respectively). Body size, intensity of haematozoan infections and leucocyte differentials were all unrelated to fault bars; however, birds with many fault bars were in poor body condition during prelaying (males) and incubation (males and females). 3. Individual kestrels tended to be consistent in the number of feathers with fault bars from year to year. 4. The percentage of feathers with fault bars was not associated with the timing of arrival in spring or prey abundance per territory; however, birds of both sexes with many bars were less likely to breed. Birds paired non-randomly, as mates tended to have a similar frequency of fault bars. Males and females with many bars had significantly later clutch initiation dates, but there were no negative consequences regarding clutch size or egg size. 5. Female kestrels with many fault bars had lower survival probabilities. Both sexes were also less likely to be recaptured in years following initial banding if they had many bars, suggesting that they were more likely to emigrate from the study area temporarily. 6. Fault bars on feathers appear to be indicative of an individual’s susceptibility to stress, and are useful in predicting components of fitness. The use of fault bars is a promising tool as they are easy to evaluate and can be assessed on live or dead birds, on moulted feathers and on individuals repeatedly over time.