1. Metabolic response of Bacillus sphaericus 1593M for dual-substrate limitation in continuous and total-cell-retention cultures
- Author
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R. K. Fernando, K. Jenny, Sankaranarayanan Meenakshisundaram, R. Sachidanandham, G. Suresh, and Kunthala Jayaraman
- Subjects
Bacillaceae ,biology ,fungi ,Biomass ,General Medicine ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Bacillales ,Bacillus sphaericus ,Chemically defined medium ,chemistry.chemical_compound ,Biochemistry ,Biosynthesis ,chemistry ,Energy source ,Bacteria ,Biotechnology - Abstract
A chemically defined medium has been developed to support the growth and the production of mosquito larvicidal factor(s) (MLF) of Bacillus sphaericus 1593M. On the basis of the data of steady-state continuous cultures, it has been understood that acetate can serve as a sole carbon and energy source for B. sphaericus 1593M. Utilization of acetate by B. sphaer-icus 1593M and the production of MLF are further enhanced by the addition of glutamate at low concentrations, both in steady-state continuous as well as in total-cell-retention cultures (TCRC). A two-step TCRC procedure resulted in better biomass and MLF production by B. sphaericus 1593M. It was also found that glutamate can serve as a carbon source as well as a growth factor in the presence of acetate and hence is a partially substitutable carbon source.
- Published
- 1997
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