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The structure of blood neutrophils, eosinophils, monocytes, and lymphocytes and differential white blood count in adult rats were studied over 120 days after a single intravenous injection of magnetoliposomes based on nanomagnetite. Magnetoliposomes had no effect on the structure of neutrophils, eosinophils, monocytes, and lymphocytes. At the same time, injection of a suspension of magnetoliposomes based on magnetite nanoparticles led to a decrease in lymphocyte count and an increase in the count of monocytes and band and segmented neutrophils in the blood. These changes were transient and the parameters returned to normal by day 40-60 after injection.

Chitosan-modified magnetic nanoparticles are a promising basis for the creation of new diagnostic agents and therapeutic drugs. The cells of erythroid, granulocytic, monocytic, lymphocytic, and megakaryocytic lineages of bone marrow of mature rats were investigated for 120 days after single injection of 0.14 g per 1 kg body weight chitosan-modified magnetic nanoparticles (Fe3O4). Light microscopy was applied to describe the structure of hematopoietic cells and morphometric study was performed on Romanowsky—Giemsa stained smears to measure cells size (µm) and relative number (%). Comparison of the biological effects of nonmodified and modified magnetite nanoparticles on hematopoietic cells of bone marrow revealed the advantages of modification. Chitosan-modified magnetite nanoparticles did not affect the structure of hematopoietic cells in the rat bone marrow, and their injection produced a reversible increase in the relative number of monocytes, stab neutrophils and segmented neutrophils, as well as polychromatic and oxyphilic normoblasts.

Aberrant methylation of regulation regions of tumorsuppressor genes is showed for many cancer diseases. In course of this modification an enzyme DNMT3 methylates RCGY sites in CpG-islands of regulation regions producing R(5mC)GY sites. Earlier we developed GLAD-PCR assay to determine R(5mC)GY site in a definite position of human genome. In this work we have applied GLAD-PCR assay to determine R(5mC)GY sites in regulation regions of ESR1 and ELMO1 tumor-suppressor genes. We have studied a fragment of first exon of ELMO1 gene and a part of ESR1 promoter region in DNA preparations from malignant cell line SW837 and colorectal tumor samples. We have checked four sites in each region and found two highly methylated sites: GCGC in first exon of ELMO1 gene and GCGT in promoter region of ESR1 gene. Site GCGT is weakly methylated in healthy tissues and more methylated in the most of colorectal samples. Site GCGC is not methylated in healthy tissues and significantly methylated in 60% of colorectal samples. A possibility to use GLAD-PCR assay for cancer diagnostics is discussed.

The study involved an analysis of the dynamics of dermatological and oncological diseases [epithelial malignant skin neoplasms (С44), malignant skin melanoma (С43)] conducted among the population of the town of Seversk in 1970- 2009. A growth in the incidence rate of malignant skin neoplasms in 2000-2009 was revealed; it was established that such data exceed similar indices for the Tomsk region

Oncological morbidity among the male workers of the Siberian Group of Chemical Enterprises (SGCE) in 1970—2005 was studied. Increased related risk (in comparison with the standard) of malignant neoplasms of resiratory and digestive tract, urinary ways and genitals was found. On the basis of the received results proposals on perfection of system of health protection of the man's personnel of the enterprises of nuclear branch are made.

Study of risk acute myocardial infarction (AMI) development in persons (80,2% males) exposed to long-term low doses ionizing radiation and chemical factors.Prospective cohort study of AMI morbidity in period 1998—2006 among the personal, exposed to long-term professional radiation and chemical factors.Risk AMI development increase in group of male personel exposed combined radiation and chemical factors. The obtained results indicate that possible summation of «traditional» AMI development risk factors and radiation factor negative influence on endothelial tissue.

Leukemia (71,2%) brings the basic contribution to the structure of hemoblastosis. The indices of leukemia disease both boys and girls of Seversk exceed the same ones in Tomsk in the age groups of 0-4 and 10-14; though according to the reliable information it is shown only at boys. It is established that the boys morbidity of hemoblastosis in Seversk exceeds the same one in Tomsk only in the age group of 0-4.

A cancer incidence in general and in its separate nosologic forms (stomach cancer, liver cancer, cancer of lung, skin cancer, breast cancer, cervical cancer, prostate cancer, thyroid gland cancer and hemoblastosis cancer) has been studied in the population of Tomsk and Tomsk region within 1990—2001 taking into account ages and sexes. The dynamics of the cancer incidence in general and in separate forms of malignant neoplasms has been cited and the prognosis of this process up to 2007 has been done. An analysis of the main risk factors has been made.

There was analyzed cancer mortality of residents of Seversk, located close to the world's largest complex of enterprises of the nuclear industry - the Siberian Chemical Enterprise (the period from 01.01.1970 till 31.12.2005 For comparison there were used rates of cancer mortality in the nearest town (Tomsk), similar in climate-geographical, economic and social conditions. The study found that cancer mortality of Seversk residents during the period 1970-2005 increased in 3.2 times (from 70.2 to 223.5 per 100,000). Among the causes of cancer mortality in men there were prevailed tumors of the digestive system as well as blood and lymphatic tissues. In women, the leading causes of death were tumors of the digestive system, the genitals and the breast. Standardized rates of cancer mortality in the Seversk population were lower than in residents of Tomsk, except female mortality due to hematological malignancies and malignant tumors of the urinary tract and genitals.

The article presents the results of analysis of dynamics of health indicators of population of industrial town (medical demographic indicators, disability, morbidity of social hygienically important diseases) during 1970-2010. The classified administrative territorial municipality of Seversk constructed near the Siberian chemical industrial center, the internationally first-rate complex of nuclear industry enterprises was used as a research base. It is demonstrated that dynamics of health indicators of studied population had such negative tendencies as rapid population ageing, population loss due to decrease of natality and increase of mortality (population of able-bodied age included), prevalence of cardio-vascular diseases, malignant neoplasms and external causes, chronization of diseases. The established tendencies are to be considered in management decision making targeted to support and promote population health in industrial towns.

According to a poll results, nearly 16.5% of questioned able-bodied inhabitants of industrial city mentioned unsatisfactory health state of self, with main reasons of negative environmental influence and low availability of quality medical care. Most respondents (81.1%) are characterized by poor medical activity. Over a quarter of the questioned (25.7%) are dissatisfied with provided medical care. The main factors determining medical activity and satisfaction with medical care are age, sex, employment place and income level.

The results from the research into the association between polymorphisms of genes-candidates for individual radiosensitivity and the frequency and spectrum of cytogenetic abnormalities are analyzed. The study was conducted among Siberian Group of Chemical Enterprises healthy employees exposed to professional irradiation in a dose range of 100-300 mSv. Genotyping of DNA samples from 96 employees was carried out by oligonucleotide microarray: "Cancer_SNP_Panel GT-17-211" ("Illumina") containing 1, 421 SNP-markers (Single Nucleotide Polymorphisms) of 406 genes. The standard cytogenetic analysis was performed in the entire examined group. We have also analyzed the association of these SNP with the frequencies of aberrant cells and following chromosomal aberrations: single chromatid fragments, chromatid exchanges, paired fragments, dicentric, ring and frequencies, translocations. We have found that 40 SNP (rs1800389, rs1051690, rs2392221, rs1041163, rs2114443, rs6083, rs1760904, rs4986894, rs488133, rs7462102, rs11249938, rs34206126, rs33945943, rs34324628, rs5742694, rs978458, rs5742667, rs2373721, rs2162679, rs889162, rs2233679, rs2010457, rs2873950, rs1574154, rs10934500, rs4688046, rs10934503, rs4624596, rs2288729, rs4227, rs1367696, rs751087, rs1269486, rs1149901, rs1800404, rs887477, rs696405, rs751087, rs81 92284, rs312016) are associated with the frequency of different types of chromosomal abnormalities (p-value with FDR of Benjamini-Hochberg--equal less than 0.05). 24 SNP (underlined) are associated with more than one type of chromosome abnormalities. In the future, we are going to confirm the results in further studies on the cohort of more than 600 persons.

The protection and promotion of population's health is a necessary condition for the provision of national security, stable economic growth and preservation of the Russian way of life. While working out the strategy of the protection and promotion of population's health, we analyzed the dynamics of the basic indicators of population's morbidity and also studied the self-estimation of health status and the personal medical activity of regional population. As a result of this, the increase in the prevalence of socially significant diseases against some reduction in the levels of primary and general morbidity was noted. The results of a sociological survey showed that personal medical activity of citizens is not high and ineffective, and factors indicated by respondents as reasons for ill health are potentially manageable. In this connection the preventive activity should be, first of all, aimed at changing the behavior of population in relation to one's own health and the minimization of the negative effect of manageable risk factors.

Investigations aimed at the creation of a standard kit for the synthesis of 99mTc-labeled ciprofloxacin (fluoroquinolone antibiotic) have been carried out. For isotope labeling, eluate from a technetium-99m generator was added to dry sterile mixtures of ciprofloxacin hydrochloride (CFH) with a reducing agent (tin chloride, SnCl2) in various ratios. The mixtures were incubated for 20 min at room temperature. The influence of components of the reaction mixture on the amount of radiochemical impurity in the resulting radiopharmaceuticals was estimated using TLC. Three 99mTc-containing complexes and a colloid related to the hydrolysis of tin compounds were formed. The tin content in each complex has been determined by introducing a 117mSn isotope label. Ways to decrease colloid formation by changing the pH of the medium and by reducing the SnCl2 concentration in the kit have been investigated. The kit composition has been optimized. It is established that radiopharmaceuticals containing less than 0.175 mg SnCl2 per 5 mg CFH can be used without additional filtration. Tests on experimental animals (rabbits) with model inflammation in soft tissues showed the functional suitability of 99mTc-labeled ciprofloxacin for diagnosis of infectious-inflammatory processes.

Dynamics of incidence of myocardial infarction among adult (older than 20 years) population of Seversk in 1998-2006 was studied according to WHO program "Myocardial Infarction Register" updated in concordance with criteria of Joint European Society of Cardiology/American College of Cardiology Committee for the Redefinition of Myocardial Infarction (2002). Incidence of myocardial infarction in the studied population had steady tendency to growth with equal contribution of such components as first and recurrent myocardial infarction what was related to progressive aging of population. Hospital myocardial infarction mortality showed steady tendency to decline evidencing for improvement of quality of medical service.

to study risk factors of arterial hypertension (AH), their significance for AH pathogenesis in atomic power plants employees occupationally exposed to long-term ionizing radiation in a "low" dose range (cumulative external radiation doses in the range up 1 Sv). The case-control study was carried in the cohort of workers aged 40-50 ever employed at the Siberian Group of Chemical Enterprises (SGCE) between 2003-2005. 970 persons were examined by standardized epidemiological methods. "Case" (n = 359) was found to be a recent AH case (less than 3 years) in SGCE employees whose average length of service is 3 years. "Control" (n = 611) - healthy men of the same age, length of service and working conditions. Risk of AH development (odds ratio (OR) = 1.6 (1.1; 2.37) was found to be higher in radiation workers in comparison with non-radiation ones without any linear dependence of risk upon cumulative external gamma-radiation dose in the range under study (OR in a cumulative external radiation dose range 7.3-21.3 mSv = 1.6 (0.96; 2.51); OR in a cumulative external radiation dose range 21.4-68.5 mSv = 1.7 (1.04; 2.67); OR in a cumulative external radiation dose range 68.6-864 mSv = 1.6 (1.01; 2.57). Not only traditional risk factors such as: obesity (OR = 7.1 (5.06; 10.1), frequent alcohol usage (OR = 1.3 (1.06; 1.63)), dyslipidemia (OR = 2.1 (1.61; 2.08)); hyperhomocisteinemia (OR = 1.4 (1.09; 1.72)); psychoemotional (OR = 1.4(1.11; 1.89)), hereditary aggravation (OR = 2.2 (1.75; 2.9)) in SGCE personnel, but also technogenic explosion descriptions are related to AH development. The long-term occupational external gamma-radiation in a dose range up 1 Sv potentiates a negative influence of CVD traditional risk factors (for hypercholesterinemia OR = 1.9 (1.3; 2.9), for AH OR = 3.1 (2.2; 4.6)) that leads to increase in risk coefficients for 30-60% in persons exposed to traditional and technogenic factors impact (for hypercholesterinemia and ionizing radiation OR = 2.5 (1.7; 3.2); for AH and IR OR = 3.9 (2.8; 5.2)) in comparison with non-radiation personnel.

A selective cross-sectional study based on Regional Medico-Dosimetric Register was carried out in some Siberian Group of Chemical Enterprises (SGCE) workers (aged 40-50) with the aim of studying the most important risk factors prevalence for cardiovascular diseases development. 2010 persons examined with the use of standardized epidemiological methods made up 32.6% of total SGCE personnel aged 40-50.the study of risk factors of cardiovascular diseases, their prevalence, intensity and significance in atomic power plants workers exposed to occupational long-term ionizing radiation in a "low" dose range (cumulative doses in the range up to 1 Sv). The study of prevalence of risk factors for CVDs development in men occupationally exposed to ionizing radiation revealed frequent changes in the type of work performed, higher levels of psychoemotional tension, as well as high prevalence of low levels of high-density lipoprotein cholesterol (HDL-cholesterol) and obesity. Women workers of the main production were found to have hypercholesterolaemia more often than those of the auxiliary production. Annual health checks of nuclear workers should be extended to include waist and hips measurement, body mass index (BMI) calculation, lipid factors evaluation, as well as stress tests (bicycle and/or stress echocardiography) in case there are three or more risk factors for CVDs for workers of the main production.

Hemoblastosis and leukemia morbidity has been analyzed in the townsfolk of Seversk (in particular, Seversk residents and Siberian Group of Chemical Enterprises (SGCE) personnel). The standardized relative risk ratios for hemoblastosis morbidity in menes and womens of Seversk were found to be significantly higher in comparison with the regional standard ratios (in 1.8 and 1.5 times correspondingly). Hemoblastosis and leukemia morbidity rate in SGCE males is significantly higher in comparison with the similar regional standard ratios. Leukemia morbidity dependence due to cumulative external gamma-radiation in a dose range up to 1 Sv has not been found in SGCE personnel, without regard to chronic lymphatic leukemia.

According to the data of cohort and to the "case-control" studies, dynamics of an acute myocardial infarction (AMI) morbidity during the period 1998-2002 and role of radiation factor in AMI development among personnel of radiation dangerous plants (by the example of Siberian Group of Chemical Enterprises (SGCE) exposed to a long-term impact of ionizing radiation in the range of "low" doses) were being carried out. It was determined that in personnel of the main production (working in contact with IR sources) the gradient increase of AMI morbidity was registered during the study period. Personnel of the main production in comparison to personnel of support production (correlating age, sex and examination level) have statistically significant increase of standardized relative risk coefficients of AMI development. When evaluating standardized relative risk coefficient depending on the cumulative dose of external y-radiation, it was impossible to determine the reliance between the rate of increase of radiation dose and standardized relative risk coefficient. According to logistic regression, was determined that individual features of dose load formation are of great significance in AMI development for the personnel of the main production. Absolute values of standardized regression coefficients peculiar to the values characterizing technogenic impact, exceed correspondent values for "traditional" risk factors to be the components of the regression equation, two-three times as much. The combination of these factors allowed to prognosticate AMI development in 94.3% cases. Obtained data made possible to conclude that in pathogenesis of acute coronary diseases ionizing radiation acts as a factor that aggravate negative influence of "traditional" risk factors of cardio-vascular diseases through pleiotropic effects.

The authors presented results of single-stage cross study of randomized select from a cohort of long seniority workers of isotope separation plant in Siberian Chemical Enterprise, with studies of inflammation markers, vascular and platelet hemostasis, serum lipid parameters, calculated atherogeneity coefficient, homocysteine levels. Serum atherogeneity coefficient and inflammation markers levels (fibrinogen, C-reactive protein) could be considered as the most informative laboratory indicators of underlying cardiovascular diseases in individuals without ischemic heart disease. Among a list of standard inductors of platelet aggregation, collagen and epinephrine should be considered preferential. Increased platelet aggregation requires disaggregant therapy, especially in smokers. Increased homocysteine level is associated with high frequency of atherosclerotic plaques and smoking.

Model of integrated social hygienic evaluation of malignant neoplasm morbidity among population is discussed. The model includes such technologic components as overall medical demographic, economic geographic and sanitary hygienic characteristics of region. Comparative analysis of prevalence of oncological pathology depending on both age- and gender-specific and localization characteristics considering demographic characteristics of territories is included. Medical statistical modeling of development of malignant neoplasm (including genetic, ontogenetic, accumulative and ecological models), risk factors analysis and forecasting of trends in development of oncological pathology are applied. Every evaluation stage is defined, including systematic approaches and set of indicators.

Possibilities of correction and prophylaxis. The authors presented results of study covering workers of radiation-dangerous plants of Siberian Chemical Enterprise, who underwent long-term exposure to outer and inner radiation in "low" doses range during the occupational activity. Target-systems for radiation effects were proved to be lipid peroxidation and antioxidant defence systems, cell energy metabolism--severity of disorders in which is correlated with degree of radiation exposure.

Medico-dosimetric register is an optimal model of epidemiological studies on evaluation of ionizing radiation effects. Regional medico-dosimetric register (RMDR) is a system of interrelating information blocks including data on Siberian Group of Chemical Enterprises (SGCE) personnel. At present SGCE personnel and Seversk residents RMDR database includes information on 138496 persons, 65538 of which are SGCE workers. SGCE personnel and Seversk residents RMDR is a scientific base for researches with the aim of evaluating long-term ionizing radiation effects in a "low" dose range. Information on mortality and morbidity rate as well as "thematic" registers of the main diseases potentiates in evaluating the spectrum of somatic stochastic effects and radiogenic risks in SGCE workers and Seversk residents as well as their offsprings. A practical significance of RMDR database is the formation of the main diseases "risk" groups depending on definite risk factors in certain groups that provides targeted diagnostic and preventive therapy both among high-dose establishments' workers and residents living near-by.

There was analyzed single nucleotide polymorphisms of DNA excision repair enzyme genes hOGG, XPD, XPG, XRCC1 in 98 Siberian Group of Chemical Enterprises cancer patients and 148 healthy donors. No association was observed between the analyzed polymorphisms and malignant tumors in both control and subgroup (under study) of persons exposed to occupational ionizing radiation. Heterozygosis for the genes hOGG and XPD was found to be a protective factor to malignant tumors in exposed persons: the odds ratio = 0.42 (95% CI 0.18-0.98; p = 0.044) for the 326Ser/Cys genotype of the hOGG gene and 0.48 (95% CI 0.23-0.99; p = = 0.047) the 751Lys/Gln genotype of the XPD gene. The data obtained show a possible modifying role of the hOGG and XPD gene polymorphisms for malignant tumors risk in exposed persons.