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2 results on '"RHVD"'

1. Synergistic effects of cyclic strain and Th1-like cytokines on tenascin-C production by rheumatic aortic valve interstitial cells.

Author

Jiang, L., Wei, X. F., Yi, D. H., Xu, P., Liu, H., Chang, Q., Yang, S. M., Li, Z. F., Gao, H. B., and Hao, G. J.

Subjects
*CYTOKINES, *AORTIC valve, *IMMUNOREGULATION, *EXTRACELLULAR matrix, *MESSENGER RNA
Abstract

Tenascin-C (TN-C) is a key component of extracellular matrix (ECM) and its expression process is poorly understood during rheumatic heart valvular disease (RHVD). In this study, we found that interferon (IFN)-γ, tumour necrosis factor (TNF)-α and TN-C concentrations in patients with RHVD were significantly higher than in normal controls. More IFN-γ receptors and TNF receptors were found being expressed on rheumatic aortic valves interstitial cells than on non-rheumatic ones and their expression was patients' sera dependent. Antibodies neutralizing IFN-γ or TNF-α could attenuate patients' sera-induced TN-C transcription by isolated rheumatic aortic valves interstitial cells. By application with different protein kinase inhibitors, we found that combined with cyclic strain, TNF-α and IFN-γ induced TN-C transcription through the RhoA/ROCK signalling pathway. At the same time, p38 mitogen-activated protein kinase was involved in TNF-α and IFN-γ induced TN-C transcription. TNF-α also increased TN-C mRNA level by additional PKC and ERK 1/2 activation. Our finding revealed a new insight into ECM remodelling during RHVD pathogenesis and new mechanisms involved in the clinical anti-IFN-γ and anti-TNF-α therapy. [ABSTRACT FROM AUTHOR]

Published
2009
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2. Characterisation of a non-pathogenic and non-protective infectious rabbit lagovirus related to RHDV

Author

Jacqueline Gelfi, Giuliana Botti, Ghislaine Le Gall-Reculé, Alain Roobrouck, F. Zwingelstein, Marie-Philippe Fages, Antonio Lavazza, Stéphane Bertagnoli, Jacky Aubineau, Stéphane Marchandeau, Agence Nationale de Sécurité Sanitaire - ANSES (FRANCE), Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Instituto Zooprofilattico Sperimentale della Lombardia et dell' Emilia Romagna - IZSLER (ITALY), Office National de la Chasse et de la Faune Sauvage - ONCFS (FRANCE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Office National de la Chasse et de la Faune Sauvage (ONCFS), and Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna 'Bruno Ubertini' (IZSLER)

Subjects
Hemorrhagic Disease Virus, Rabbit, 040301 veterinary sciences, Sequence analysis, Cross Protection, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Sequence Homology, Cuniculus, Rabbit, Antibodies, Viral, Bunyaviridae Infections, Virus, 0403 veterinary science, 03 medical and health sciences, Phylogenetics, RHVD, Virology, Calicivirus, Animals, Cluster Analysis, Phylogeny, 030304 developmental biology, 0303 health sciences, biology, Phylogenetic tree, Lagovirus, Non-pathogenic virus, RHDV, Sequence Analysis, DNA, 04 agricultural and veterinary sciences, biology.organism_classification, 3. Good health, Médecine vétérinaire et santé animal, Capsid, Carrier State, Tissue tropism, RNA, Viral, RNA, Oryctolagus, Rabbits
Abstract

International audience; The existence of non-pathogenic RHDV strains was established when a non-lethal virus named rabbit calicivirus (RCV) was characterised in 1996 in Italy. Since then, different RNA sequences related to RHDV have been detected in apparently healthy domestic and wild rabbits, and recently a new lagovirus was identified in Australia. We have characterised from seropositive healthy domestic rabbits a non-lethal lagovirus that differs from RHDV in terms of pathogenicity, tissue tropism and capsid protein sequence. Phylogenetic analyses have revealed that it is close to the Ashington strain and to the RCV, but distinct. We proved experimentally that it is infectious but non-pathogenic and demonstrated that, contrary to the other described non-pathogenic lagoviruses, it induces antibodies that do not protect against RHDV. Our results indicate the existence of a gradient of cross-protection between circulating strains, from non-protective, partially protective to protective strains, and highlight the extent of diversity within the genus Lagovirus.

Published
2011
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