132 results on '"ROBBINS, Robert T."'
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2. Advancements in breeding, genetics, and genomics for resistance to three nematode species in soybean
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Kim, Ki-Seung, Vuong, Tri D., Qiu, Dan, Robbins, Robert T., Grover Shannon, J., Li, Zenglu, and Nguyen, Henry T.
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- 2016
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3. Registration of ‘S16‐3747GT’: A high‐yielding determinate maturity group V soybean cultivar with broad biotic and abiotic stressors tolerance
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Chen, Pengyin, primary, Shannon, J. Grover, additional, Canella Vieira, Caio, additional, Nascimento, Emanuel Ferrari do, additional, Ali, M. Liakat, additional, Lee, Dongho, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Mitchum, Melissa Goellner, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Usovsky, Mariola, additional, Li, Shuxian, additional, Gillen, Anne M., additional, Smith, James R., additional, Mengistu, Alemu, additional, Zhang, Bo, additional, Mozzoni, Leandro Angel, additional, Robbins, Robert T., additional, and Moseley, David, additional
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- 2022
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4. ‘S16‐7922C’: A semi‐determinate maturity group IV conventional soybean cultivar with high yield and broad disease resistance
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Chen, Pengyin, primary, Shannon, J. Grover, additional, Lee, Dongho, additional, Vieira, Caio Canella, additional, Ali, Md Liakat, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Mitchum, Melissa Goellner, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Usovsky, Mariola, additional, Li, Shuxian, additional, Gillen, Anne M., additional, Mengistu, Alemu, additional, Zhang, Bo, additional, Mozzoni, Leandro Angel, additional, Robbins, Robert T., additional, and Moseley, David, additional
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- 2022
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5. Registration of ‘S16‐11651C’, a conventional soybean cultivar with high yield, resistance to multiple diseases, and broad adaptation
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Chen, Pengyin, primary, Ali, Md Liakat, additional, Shannon, J. Grover, additional, Canella Vieira, Caio, additional, Lee, Dongho, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Scaboo, Andrew, additional, Usovsky, Mariola, additional, Nguyen, Henry, additional, Mitchum, Melissa, additional, Li, Zenglu, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Li, Shuxian, additional, Gillen, Anne M., additional, Mengistu, Alemu, additional, Robbins, Robert T., additional, Mozzoni, Leandro Angel, additional, Zhang, Bo, additional, Smith, James Rusty, additional, Buckley, J. Blair, additional, and Moseley, David, additional
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- 2022
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6. ‘ShowMeSoy 4301’: High‐yielding soybean with multiple disease resistance and elevated seed oil content
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Chen, Pengyin, primary, Shannon, J. Grover, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Canella Vieira, Caio, additional, Ali, Md Liakat, additional, Lee, Dongho, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Mitchum, Melissa Goellner, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Usovsky, Mariola, additional, Li, Shuxian, additional, Mengistu, Alemu, additional, Zhang, Bo, additional, Mozzoni, Leandro Angel, additional, and Robbins, Robert T., additional
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- 2022
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7. Registration of ‘S16‐15170C’ soybean: A high‐yielding indeterminate maturity group V cultivar with wide adaptability and multiple disease resistance
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Chen, Pengyin, primary, Shannon, J. Grover, additional, Canella Vieira, Caio, additional, Ali, MD Liakat, additional, Lee, Dongho, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Mitchum, Melissa Goellner, additional, Averitt, Ben, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Usovsky, Mariola, additional, Li, Shuxian, additional, Gillen, Anne M., additional, Mengistu, Alemu, additional, Zhang, Bo, additional, Mozzoni, Leandro Angel, additional, Robbins, Robert T., additional, and Moseley, David, additional
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- 2022
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8. Registration of ‘S15‐10434C’ soybean cultivar with high yield, resistance to multiple diseases, and wide adaptation
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Chen, Pengyin, primary, Ali, M. L., additional, Shannon, Grover, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Canella Vieira, Caio, additional, Lee, Dongho, additional, Scaboo, Andrew, additional, Usovsky, Mariola, additional, Nguyen, Henry T., additional, Mitchum, Melissa G., additional, Li, Zenglu, additional, Bond, Jason, additional, Meinhardt, Clinton, additional, Li, Shuxian, additional, Mengistu, Alemu, additional, Robbins, Robert T., additional, Mozzoni, Leandro A., additional, Zhang, Bo, additional, Smith, James R., additional, and Buckley, Blair, additional
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- 2022
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9. Registration of ‘S16‐5540GT’ soybean cultivar with high yield, resistance to multiple diseases, elevated protein content, and wide adaptation
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Chen, Pengyin, primary, Ali, Md Liakat, additional, Shannon, J. Grover, additional, Canella Vieira, Caio, additional, Lee, Dongho, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Scaboo, Andrew, additional, Usovsky, Mariola, additional, Nguyen, Henry T., additional, Mitchum, Melissa G., additional, Li, Zenglu, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Li, Shuxian, additional, Mengistu, Alemu, additional, Robbins, Robert T., additional, Mozzoni, Leandro A., additional, Zhang, Bo, additional, Smith, James R., additional, and Buckley, J. Blair, additional
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- 2022
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10. Registration of ‘S13‐10592C’, a high‐yielding soybean cultivar with resistance to multiple diseases and elevated oil content
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Chen, Pengyin, primary, Shannon, J. Grover, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Canella Vieira, Caio, additional, Ali, MD Liakat, additional, Lee, Dongho, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Mitchum, Melissa Goellner, additional, Bond, Jason P., additional, Meinhardt, Clinton G., additional, Usovsky, Mariola, additional, Li, Shuxian, additional, Mengistu, Alemu, additional, Zhang, Bo, additional, Mozzoni, Leandro Angel, additional, and Robbins, Robert T., additional
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- 2022
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11. Classification Methods and Identification of Reniform Nematode Resistance in Known Soybean Cyst Nematode-Resistant Soybean Genotypes
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Usovsky, Mariola, primary, Robbins, Robert T., additional, Fultz Wilkes, Juliet, additional, Crippen, Devany, additional, Shankar, Vijay, additional, Vuong, Tri D., additional, Agudelo, Paula, additional, and Nguyen, Henry T., additional
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- 2022
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12. Registration of ‘S16‐11644C’: A maturity group IV soybean cultivar with high‐yielding performance and broad disease resistance
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Chen, Pengyin, Shannon, J. Grover, Lee, Dongho, do Granja, Matheus Ogando, Ali, Md Liakat, Vieira, Caio Canella, Lee, Yi‐Chen, do Nascimento, Emanuel Ferrari, Scaboo, Andrew, Crisel, Melissa, Smothers, Scotty, Clubb, Michael, Selves, Stewart, Nguyen, Henry T., Li, Zenglu, Mitchum, Melissa Goellner, Averitt, Ben, Bond, Jason P., Meinhardt, Clinton G., Usovsky, Mariola, Li, Shuxian, Smith, James R., Gillen, Anne M., Mengistu, Alemu, Zhang, Bo, Mozzoni, Leandro Angel, Robbins, Robert T., and Moseley, David
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‘S16‐11644C’ (Reg. no. CV‐556, PI 700010) is a semi‐determinate maturity group IV non–genetically modified (GM) soybean cultivar developed and released by the University of Missouri–Fisher Delta Research, Extension, and Education Center to fulfill the growing market demands and consumers’ preference for non‐GM soybean cultivars. S16‐11644C was tested in a total of 82 environments across 11 southern states from 2017 to 2020. It demonstrated high yield potential and broad adaptability compared with commercial and public check cultivars. S16‐11644C is resistant to soybean cyst nematode races 2 (HG Type 1.2.5.7), 3 (HG Type 5.7), and 5 (HG Type 2.5.7); southern root‐knot nematode; peanut root‐knot nematode; and charcoal rot and is a salt excluder. Seed of S16‐11644C average 410 and 224 g kg−1of seed protein and oil content on a dry weight basis, respectively, and 48.2% of meal protein content at 13% moisture level. Due to its high‐yielding genetic background with a desired early maturity, broad adaptability, disease resistance, and absence of GM events, S16‐11644C offers a unique package for soybean growers across the U.S. mid‐southern states to maximize profits through premium prices of non‐GM soybean cultivars in the niche markets and the flexibility of harvest and saving seeds for the next crop seasons. S16‐11644C is a high‐yielding, non–genetically modified soybean cultivar.S16‐11644C is resistant to soybean cyst nematode, root‐knot nematodes, and charcoal rot and is tolerant to salt.S16‐11644C was extensively tested in 82 locations across 11 states.
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- 2023
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13. Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
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Jiao, Yongqing, Vuong, Tri D., Liu, Yang, Li, Zenglu, Noe, Jim, Robbins, Robert T., Joshi, Trupti, Xu, Dong, Shannon, J. Grover, and Nguyen, Henry T.
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- 2015
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14. Longidorus kheirii n. sp. (Nematoda: Longidoridae) from Iran
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Pedram, Majid, Niknam, Gholamreza, Robbins, Robert T., Ye, Weimin, and Karegar, Akbar
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- 2008
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15. Dissecting nematode resistance regions in soybean revealed pleiotropic effect of soybean cyst and reniform nematode resistance genes
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Usovsky, Mariola, primary, Lakhssassi, Naoufal, additional, Patil, Gunvant B., additional, Vuong, Tri D., additional, Piya, Sarbottam, additional, Hewezi, Tarek, additional, Robbins, Robert T., additional, Stupar, Robert M., additional, Meksem, Khalid, additional, and Nguyen, Henry T., additional
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- 2021
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16. SSR mapping and confirmation of soybean QTL from PI 437654 conditioning resistance to reniform nematode
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Ha, Bo-Keun, Robbins, Robert T., Han, Feng, Hussey, Richard S., Soper, John F., and Boerma, H. Roger
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Soybean -- Research ,Soybean -- Genetic aspects ,Quantitative trait loci -- Analysis ,Plant immunology -- Research ,Nematode diseases of plants -- Research ,Agricultural industry ,Business - Abstract
Recently, reniform nematode (Rotylenchulus reniformis Linford and Oliveira) has been recognized to be a major problem on soybean [Glycine max (L.) Merr.] in the southern USA. Planting reniform nematode-resistant soybean cultivars is the most cost effective control method. With restriction fragment length polymorphism (RFLP) markers, Pioneer Hi-Bred International, Inc. had previously identified quantitative trait loci (QTL) on linkage group B1 (LG-B1) and another on LG-L associated with reniform nematode resistance in a cross between 'BSR101' and PI 437654. In this study, we refined the QTL locations and identified additional QTL conditioning resistance to reniform nematode in a population of 228 recombinant inbred lines (RILs) from a cross of BSR101 x PI 437654. A QTL ([R.sup.2] = 21%) conditioning reniform reproductive index (RI) was found on LG-L and was flanked by Sat 184 and Satt513. Two other QTL were identified, one on LG-B1 ([R.sup.2] = 16%) and the other on LG-G ([R.sup.2] = 8%). These two QTL acted in an epistatic manner with lines homozygous for PI 437654 alleles at both QTL providing the lowest reniform RI. The 31 RILs from BSR101 x PI 437654 that were homozygous for the PI 437654 alleles at Satt513, Satt359, and Sat_168 averaged 1077 reniform eggs and juveniles (RI = 0.63), while the 33 RILs homozygous for the BSR101 alleles averaged 10606 (RI = 6.24). By screening the RILs population of 'Prichard' x 'Anand' we confirmed that the QTL on LG-G and-B1 conditioned reniform RI.
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- 2007
17. Registration of ‘S14‐15146GT’ soybean, a high‐yielding RR1 cultivar with high oil content and broad disease resistance and adaptation
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Chen, Pengyin, primary, Shannon, Grover, additional, Scaboo, Andrew, additional, Crisel, Melissa, additional, Smothers, Scotty, additional, Clubb, Michael, additional, Selves, Stewart, additional, Vieira, Caio Canella, additional, Ali, Md. Liakat, additional, Mitchum, Melissa Goellner, additional, Nguyen, Henry, additional, Li, Zenglu, additional, Bond, Jason, additional, Meinhardt, Clinton, additional, Klepadlo, Mariola, additional, Li, Shuxian, additional, Mengistu, Alemu, additional, and Robbins, Robert T., additional
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- 2020
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18. Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA
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Ma, Xinyuan, primary, Robbins, Robert T., additional, Bernard, Ernest C., additional, Holguin, Claudia M., additional, and Agudelo, Paula, additional
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- 2019
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19. Evaluation of Soybean Germplasm for Resistance to Multiple Nematode Species: Heterodera glycines , Meloidogyne incognita , and Rotylenchulus reniformis
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Klepadlo, Mariola, primary, Meinhardt, Clinton G., additional, Vuong, Tri D., additional, Patil, Gunvant, additional, Bachleda, Nicole, additional, Ye, Heng, additional, Robbins, Robert T., additional, Li, Zenglu, additional, Shannon, J. Grover, additional, Chen, Pengyin, additional, Meksem, Khalid, additional, and Nguyen, Henry T., additional
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- 2018
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20. Plant elicitor peptides promote plant defences against nematodes in soybean
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Lee, Min Woo, primary, Huffaker, Alisa, additional, Crippen, Devany, additional, Robbins, Robert T., additional, and Goggin, Fiona L., additional
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- 2017
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21. QTL Underlying Reniform Nematode Resistance in Soybean Cultivar Hartwig
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Lee, Yi-Chen, primary, Lightfoot, David A., additional, Anderson, James, additional, Robbins, Robert T., additional, and Kantartzi, Stella K., additional
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- 2016
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22. Reaction of Soybean Cyst Nematode Resistant Plant Introductions to Root-Knot and Reniform Nematodes
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Lee, Jeong-Dong, primary, Kim, Hyun-Jee, additional, Robbins, Robert T., additional, Wrather, James A., additional, Bond, Jason, additional, Nguyen, Henry T., additional, and Shannon, J. Grover, additional
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- 2015
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23. Plant elicitor peptides promote plant defences against nematodes in soybean.
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Lee, Min Woo, Huffaker, Alisa, Crippen, Devany, Robbins, Robert T., and Goggin, Fiona L.
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SOYBEAN diseases & pests ,NEMATODE-plant relationships ,PLANT defenses ,PROMOTERS (Genetics) ,PLANT proteins ,ANGIOSPERMS ,PLANT immunology - Abstract
Summary: Plant elicitor peptides (Peps) are widely distributed among angiosperms, and have been shown to amplify immune responses in multiple plant families. Here, we characterize three Peps from soybean (
Glycine max ) and describe their effects on plant defences against two damaging agricultural pests, the root‐knot nematode (Meloidogyne incognita ) and the soybean cyst nematode (Heterodera glycines ). Seed treatments with exogenous GmPep1, GmPep2 or GmPep3 significantly reduced the reproduction of both nematodes. Pep treatment also protected plants from the inhibitory effects of root‐knot nematodes on above‐ground growth, and up‐regulated basal expression levels of nematode‐responsive defence genes. GmPep1 induced the expression of its propeptide precursor (GmPROPEP1 ), a nucleotide‐binding site leucine‐rich repeat protein (NBS‐LRR ), a pectin methylesterase inhibitor (PMEI ),Respiratory Burst Oxidase Protein D (RBOHD ) and the accumulation of reactive oxygen species (ROS) in leaves. In addition, GmPep2 and GmPep3 seed treatments up‐regulatedRBOHD expression and ROS accumulation in roots and leaves. These results suggest that GmPeps activate plant defences through systemic transcriptional reprogramming and ROS signalling, and that Pep seed treatments represent a potential strategy for nematode management. [ABSTRACT FROM AUTHOR]- Published
- 2018
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24. Taxonomic and Molecular Identification of Bakernema, Criconema, Hemicriconemoides, Ogma and Xenocriconemella Species (Nematoda: Criconematidae)
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Cordero, Marco A., Robbins, Robert T., and Szalanski, Allen L.
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Contributed Paper - Abstract
Populations of Bakernema inaequale, C. petasum, C. sphagni, C. mutabile, Ogma octangulare, Xenocriconemella macrodora and Hemicriconemoides chitwoodi were identified and re-described from different geographical areas in the continental United States and molecularly characterized. Two new species of spine nematodes Criconema arkaense n. sp. from Washington County and Lee County, Arkansas and Criconema warrenense n. sp from Warren, Bradley County, Arkansas are also described and named. Criconema arkaense is characterize by having a conspicuous lip region offset from the body with two annuli, short rounded tail with a thin cuticular sheath and subterminal anus. Criconema warrenense n. sp. has two lip region annuli about the same width, first annulus directed posteriorly, separated by a narrow neck annulus and a short conoid tail, unilobed non-folded annulus. The molecular characterization of Criconema arkaense and Criconema warrenense using ITS1 rDNA gene sequence and the molecular phylogenetic relationships of these new species along with the known spines nematodes are provided.
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- 2012
25. Enchodelus groenlandicus Thorne 1939
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Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin, and Santiago, Reyes Peña
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Enchodelus groenlandicus ,Dorylaimidae ,Nematoda ,Enchodelus ,Dorylaimida ,Animalia ,Adenophorea ,Biodiversity ,Taxonomy - Abstract
Enchodelus groenlandicus (Ditlevsen, 1927) Thorne, 1939 = Dorylaimus (Doryllium) groenlandicus Ditlevsen, 1927 = Dorylaimellus groenlandicus (Ditlevsen, 1927) Thorne & Swanger, 1936 (Fig. 1) MATERIAL EXAMINED Four females collected from one location. MEASUREMENTS See Table 1. DISTRIBUTION Collected in March, 2010 from Ahar Village, Tehran Province, Iran, in the rhizosphere of grasses. REMARKS The examined specimens fit very well with the recent redescription of this species by Guerrero et al. (2008a), although some observed morphometric differences are considered as intraspecific variation. The Iranian population has a slightly longer pharyngeal expansion (140-162 vs 111-142 µ m) and shorter female tail (19-24 vs 22-37 µ m; c = 73-104 vs 53-83; cļ = 0.4-0.6 vs 0.6-0.8). Moreover, taking into account the tripartite nature of the uterus in this species, as illustrated by Guerrero et al. (2008a), the Iranian population has a less developed pars distalis than Iberian populations, although the pars dilatata oviductus is more developed than in the Iberian specimens and female tail lacks saccate bodies (vs some very small bodies observed). One of the four females observed had two vulvae. Its morphometrics are: L = 1.89 mm, a = 23.9, b = 5.1, c = 82, cļ = 0.5, V 1 = 34.0, V 2 = 41.5, lip region diam. = 22 µ m, odontostyle length = 45 µ m, odontophore length = 53 µ m, neck length = 372 µ m, pharyngeal expansion length = 155 µ m, anal body diam. = 45 µ m and tail length = 23 µ m. This is the first report of the species in Iran., Published as part of Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin & Santiago, Reyes Peña-, 2011, Description of one new, and new data on two known, species of Enchodelus Thorne, 1939 (Dorylaimida: Nordiidae) from Iran, pp. 729-740 in Nematology 13 (6) on page 730, DOI: 10.1163/138855410X545786, http://zenodo.org/record/8114679
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- 2011
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26. Enchodelus sardashtensis Pedram & Pourjam & Robbins & Ye & Santiago 2011, sp. n
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Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin, and Santiago, Reyes Peña
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Dorylaimidae ,Enchodelus sardashtensis ,Nematoda ,Enchodelus ,Dorylaimida ,Animalia ,Adenophorea ,Biodiversity ,Taxonomy - Abstract
Enchodelus sardashtensis * sp. n. (Figs 2, 3) MATERIAL EXAMINED Ten females from one location. MEASUREMENTS See Table 3. Female Moderately slender nematodes of medium size, 1.29- 1.50 mm long. Body cylindrical, tapering towards anterior end. Habitus curved ventrad upon fixation, adopting an open C-shape. Cuticle two-layered, 2.0-3.0 µ m thick in anterior region, 1.5-3.0 µ m at mid-body and 4-5 µ m on tail, outer layer bearing very fine transverse striations which are more conspicuous at dorsal surface of tail. Lip region moderately angular, offset by a marked depression, 2.3-2.8 times as broad as high, ca one-third of body diam. at neck base, lips mostly amalgamated, labial papillae distinct, visibly protruding above lip region contour. Amphid fovea cup-shaped, opening at level of labial depression, aperture 8-10 µ m or three-fifths to twothirds of lip region diam. Odontostyle typical of genus, comparatively long and slender, 1.5-2.0 µ m diam., ca 11.5-14.0 times as long as wide, 1.5-1.8 times longer than lip region diam. and ca 1.9% of total body length. Odontophore 1.3-1.7 odontostyle lengths long, with well developed basal flanges. Guiding ring ‘double’, situated at 15-17 µ m from anterior end. Pharynx consisting of slender but muscular anterior section enlarging gradually, basal expansion 3.5-3.8 times as long as broad, ca twice as long as body diam. at its level and occupying 35- 38% of total neck length, gland nuclei located as follows: DN = 68-72, AS obscure, PS = 52-59. Nerve ring situated at 125-133 µ m from anterior end. Cardia rounded conoid, (10-17) × (10-13) µ m, almost as long as wide. Distinct dorsal, ovoid to spindle-shaped cellular mass, ca 40 µ m long, present in all specimens between cardia and proximal end of anterior ovary. Green material observed inside intestine. Genital system didelphic-amphidelphic, both branches equally and well developed, anterior 247- 285 µ m and posterior 230-270 µ m long, ovaries usually large, 95-182 µ m long, often reaching and surpassing sphincter level, oviduct 115-150 µ m or 1.9-2.5 body diam. long, joining ovary subterminally, consisting of a tubular part and a well developed pars dilatata. Oviduct and uterus separated by a distinct sphincter. Uterus 95- 145 µ m long, tripartite, i.e., consisting of a wide proximal region with distinct lumen, a more slender intermediate section with very narrow lumen, and a well developed, spheroid distal portion. One or two uterine eggs, (86-97)× (37-51) µ m, observed within one or both genital tracts, with thick shell. Vagina extending inwards two-fifths to one-half of body diam., pars proximalis (19-22) × (18- 20) µ m, with somewhat sigmoid walls and surrounded by weak musculature, pars refringens consisting of (in lateral view) two triangular sclerotisations, measuring (5-6)×(5- 7) µ m; pars distalis 4.0-5.0 µ m long. Vulva a transverse slit. Prerectum 2.5-3.5 anal body diam. long. Rectum 25- 36 µ m or almost as long as anal body diam. Tail rounded conoid, ventrally almost straight, dorsally convex, a few large and distinct saccate bodies present in ventral region. Two pairs of caudal pores, one subdorsal, one practically lateral (in one female three pores observed). * The species name refers to Sardasht, the geographical region from where it was collected. Male Unknown. TYPE HABITAT AND LOCALITY Soil in the rhizosphere of grasses, Shalmash village, Sardasht, western Azarbayjan, north-western Iran. TYPE MATERIAL Female holotype and five female paratypes deposited in the Nematode collection of the Faculty of Agriculture, University of Tarbiat Modarre, Tehran, Iran. Five female paratypes deposited with the Nematode Collection of Departamento de Biología Animal, Biología Vegetal y Ecología, University of Jaén, Spain and two paratype females deposited with USDA Nematode Collection, Beltsville, MD, USA. DIAGNOSIS AND RELATIONSHIPS The new species is characterised by its body length of 1.3-1.5 mm, lip region offset by marked depression and 13-15 µ m broad, odontostyle 22-27 µ m long or 1.5- 1.8 times the lip region diam., neck 290-310 µ m long, pharyngeal expansion 100-110 µ m long or 35-38% of total neck length, a dorsal cellular mass present between cardia and proximal end of anterior ovary, uterus tripartite and 95-145 µ m long, V = 44-48, female tail rounded conoid (19-23 µ m, c = 61-76, cļ = 0.7-0.8) and bearing saccate bodies, and male unknown. Having a comparatively small size (L * 1.5 mm), narrow lip region (up to 15 µ m) and short odontostyle (< 30 µ m), E. sardashtensis sp. n. is very similar to E. altherri Vinciguerra & De Francisci, 1973 and E. arcticus Nesterov, 1976, two close, poorly described, species (see also remarks), only known to occur in their corresponding type locations (cf., Guerrero et al., 2008). It differs from E. altherri in its shorter odontophore (35-37 vs 42-45 µ m) and total stylet length (58-65 vs 67-72 µ m), longer neck (290-310 vs 236-276 µ m), more anterior vulva (V = 44- 48 vs 50-52), shorter female tail (c = 61-76 vs 55-58), and male absent vs present, and from E. arcticus in its longer odontostyle (ca 1.5-1.8 vs 1.2 times the lip region diam.), relatively shorter pharyngeal expansion (35-38 vs ca 43% of total neck length), more anterior vulva (V = 44-48 vs 49-51), shorter female tail (19-23 vs 23-31 µ m, c = 61- 76 vs c = 48-53) with vs without saccate bodies, and male absent vs present. MOLECULAR STUDY Ribosomal DNA near-full-length small subunit 18S gene of E. sardashtensis sp. n. was sequenced and deposited in GenBank under accession number HQ404367. Sequencing on internal transcribed spacer and partial 5.8S gene was not successful. Blast search revealed this sequence is unique without identical match with any other species in GenBank. The 18S of this species had 99% identity with E. macrodorus voucher M53 from Iran ( FJ042953) with 3 bp differences over 1190 bp aligned sequences, Enchodelus sp. (AY284793) with 5 bp differences over 1311 bp aligned sequences, Enchodelus sp. (AY284792) with 9 bp differences over 1311 bp aligned sequences, Enchodelus sp. (EP207247) with 12 bp differences over 1311 bp aligned sequences, and 96% identity with E. veletensis voucher M34 from Iran ( EU477379) with 38 bp differences and 3 gaps over 1193 bp aligned sequences. Phylogenetic analysis revealed that E. sardashtensis sp. n. is closest to E. macrodorus M53 (Fig. 4) and is in the round-tail clade (Fig. 5). This 18S fragment is very conserved; E. sardashtensis sp. n. has identical sequence with E. babakicus, E. longispiculus, E. macrodorus and E. hopedorus. REMARKS Some (although minimal) doubts persist as to the true identity of this Iranian population due to the fact that the original descriptions of its most similar species, E. altherri and E. arcticus, lack many details necessary to carry out a detailed comparison. Nevertheless, the differences observed, especially those concerning the length of odontostyle/odontophore/total stylet, pharynx length and vulva position, are significant enough to support the proposal of a new taxon., Published as part of Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin & Santiago, Reyes Peña-, 2011, Description of one new, and new data on two known, species of Enchodelus Thorne, 1939 (Dorylaimida: Nordiidae) from Iran, pp. 729-740 in Nematology 13 (6) on pages 734-737, DOI: 10.1163/138855410X545786, http://zenodo.org/record/8114679
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- 2011
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27. Enchodelus longispiculus Guerrero, Liebanas & Pena-Santiago 2008
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Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin, and Santiago, Reyes Peña
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Dorylaimidae ,Enchodelus longispiculus ,Nematoda ,Enchodelus ,Dorylaimida ,Animalia ,Adenophorea ,Biodiversity ,Taxonomy - Abstract
Enchodelus longispiculus Guerrero, Liébanas & Peña-Santiago, 2008 MATERIAL EXAMINED Thirty-nine females and 27 males from three localities. MEASUREMENTS See Table 2. MOLECULAR STUDY Ribosomal DNA near-full-length small subunit 18S gene, internal transcribed spacer and partial 5.8S gene of E. longispiculus were sequenced and deposited in GenBank under the accession number HM851184. Blast search of the 2562 nucleotides yielded the highest match with other Enchodelus species. On the 18S, E. longispiculus had an identical sequence with E. babakicus voucher M32 from Iran (FJ042952). The 18S of this species had 99% identity with E. macrodorus voucher M53 from Iran (FJ042953) with 2 bp differences, Enchodelus sp. (AY284793) with 5 bp differences, Enchodelus sp. (AY284792) with 9 bp differences, Enchodelus sp. (EP207247) with 15 bp differences, and 97% identity with E. veletensis voucher M34 from Iran (EU477379) with 35 bp differences and six gaps. However, the ITS1 region of E. longispiculus had considerable variation compared with three Iranian species including E. babakicus voucher M32 (FJ042950, identities = 601 / 725 (82.9%), gaps = 27 / 725 (3.7%)), E. macrodorus voucher M53 (FJ042951, identities = 568 / 729 (77.9%), gaps = 85 / 729 (11.7%)), and E. veletensis M34 (EU477380, identities = 353 / 672 (52.5%), gaps = 70 / 672 (10.4%)). These significant differences indicate that they are all distinct species. No ITS sequence data is available to compare for other Enchodelus species from GenBank. Figure 4 is a phylogenetic tree from a multiple alignment with 1693 total characters. This tree includes some dorylaimid species with a high match from the blast search result in GenBank. This tree is consistent with our previous results (Pedram et al., 2009a, b). Enchodelus longispiculus is identical to E. babakicus and they are in the same clade with E. sardashtensis sp. n., E. macrodorus and three other unidentified Enchodelus spp. with 100% support. This clade in the Nordiidae is sister to Eudorylaimus Andrássy, 1959 in Qudsianematidae, Epidorylaimus Andrássy, 1986 in Qudsianematidae and Prodorylaimus Andrássy, 1959 in Dorylaimidae. Enchodelus veletensis voucher M34 from Iran is not monophyletic with above-mentioned Enchodelus species. Figure 5 is a phylogenetic tree from a multiple alignment with only 638 total characters, so that all species in Enchodelus from GenBank can be included to examine their relationships. This tree is also consistent with our previous results (Pedram et al., 2009a, b) with two significant clades, viz. conical tail vs rounded tail (the clade to which E. longispiculus belongs). This tree shows that the 3 ļ portion of 18S is highly conserved. Many Enchodelus species share identical DNA sequences although they can be easily differentiated morphologically. * Total range of several populations (taken from Guerrero et al., 2008a). DISTRIBUTION Found in three localities, in the rhizosphere of grasses, in Kaleibar, Eastern Azarbayjan, Chorreh village, Roudbar, Gilan province, and Astara, Gilan province, Iran. Collected in July, August and November, 2009. REMARKS The available data on E. longispiculus, i.e., its original description from the Iberian Peninsula (Guerrero et al., 2008b) and its subsequent report from Romania (Ciobanu et al., 2010), suggest that it might be a widely distributed species. It shows important intraspecific variations, especially in some morphometric features, e.g., body size (L = 1.07-1.87 mm), odontostyle length (24-34 µ m), vulva position (V = 41-51), etc. The three Iranian populations of the species herein studied fit well with those previously known, but it is morphometrically closer to Romanian material in its body size. Moreover, the range of some measurements is wider than that observed so far (although almost always overlaps it) in such parameters as lip region 13-15 µ m (vs 14-18 µ m in previous studies), body diam. at mid-body 40-55 µ m (vs 47-71 µ m) and tail length 15-37 µ m (vs 17-29 µ m). This is the second report of the species in Asia, although the first one in Uzbekistan by Tulaganov and Usmanova (1978) is rather problematic since no description or illustration was provided (cf. Andrássy, in lit.)., Published as part of Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin & Santiago, Reyes Peña-, 2011, Description of one new, and new data on two known, species of Enchodelus Thorne, 1939 (Dorylaimida: Nordiidae) from Iran, pp. 729-740 in Nematology 13 (6) on pages 730-734, DOI: 10.1163/138855410X545786, http://zenodo.org/record/8114679
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- 2011
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28. Enchodelus macrodorus Thorne 1939
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Pedram, Majid, Niknam, Gholamreza, Guerrero, Pablo, Ye, Weimin, and Robbins, Robert T.
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Enchodelus macrodorus ,Dorylaimidae ,Nematoda ,Enchodelus ,Dorylaimida ,Animalia ,Adenophorea ,Biodiversity ,Taxonomy - Abstract
Enchodelus macrodorus (de Man, 1880) Thorne, 1939 = Dorylaimus macrodorus de Man, 1880 = Dorylaimus (Doryllium) macrodorus (de Man, 1880) Ditlevsen, 1928 = Dorylaimellus macrodorus (de Man, 1880) Thorne & Swanger, 1936 (Fig. 5) MEASUREMENTS See Table 1. REMARKS The morphometrics of the Iranian population are within the accepted values for the species as stated by Guerrero et al. (2008a), with only a small broadening of some measurement ranges such as odontophore length (46-57 vs 42-55 µ m), body diam. (68-92 vs 56-86 µ m; a = 17-24 vs 19-32) and tail length (17-23 vs 22-37 µ m; cļ = 0.4-0.6 vs 0.5-0.7). The following features have been considered of special relevance when comparing this species with E. babakicus n. sp.: i) labial constriction absent vs present; ii) vulva strikingly more anterior (V = 41.5-44.5 vs 44- 49); iii) slightly shorter uterus (119-149 vs 130-175 µ m or 1.6-2.2 vs 1.9-2.5 times corresponding body diam.); iv) males not found vs common (all Enchodelus males found in the sample had a lip morphology typical of E. babakicus n. sp. which had a sex ratio of 1.23 females per male). This evidence supports the suggestion by Guerrero et al. (2008a) that E. macrodorus males are very rare. In that study, 30 records of E. macrodorus were analysed, 12 of these being considered as reasonably confirmed although only three reported the presence of males. Detailed descriptions of E. macrodorus males are not available in the literature and there are no data on important measurements, such as spicule length, which could support a more accurate diagnosis. Individuals identified as E. macrodorus were collected from the same sample as E. babakicus n. sp. and represent the first report of this species from Iran., Published as part of Pedram, Majid, Niknam, Gholamreza, Guerrero, Pablo, Ye, Weimin & Robbins, Robert T., 2009, Morphological and molecular characterisation of Enchodelus babakicus n. sp. and E. macrodorus Thorne, 1939 (Nematoda: Nordiidae) from Iran, pp. 895-907 in Nematology 11 (6) on page 902, DOI: 10.1163/156854109X430563, http://zenodo.org/record/8114788
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- 2009
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29. Enchodelus babakicus Pedram & Niknam & Guerrero & Ye & Robbins 2009, n. sp
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Pedram, Majid, Niknam, Gholamreza, Guerrero, Pablo, Ye, Weimin, and Robbins, Robert T.
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Enchodelus babakicus ,Dorylaimidae ,Nematoda ,Enchodelus ,Dorylaimida ,Animalia ,Adenophorea ,Biodiversity ,Taxonomy - Abstract
Enchodelus babakicus * n. sp. (Figs 1-4) MEASUREMENTS See Table 1. DESCRIPTION Female Moderately slender nematodes of medium size, 1.21- 1.56 mm long. Habitus slightly curved ventrad when heat relaxed, adopting an open C-shape. Body cylindrical, tapering gradually towards both ends but more so anteriorly. Cuticle two-layered, external layer ca 1.0-2.0 µ m thick along entire body, internal layer 2.0-3.0 µ m thick in anterior region, 1.0-1.5 µ m at mid-body and 5.0-7.0 µ m on tail. Cuticle with fine but distinct transverse striations, especially visible at neck and tail regions. Lateral cords granular, forming 15-20% of body diam. at mid-body. Lip region rounded, clearly separated from adjacent body by a distinct constriction, 2.4 times as broad as high or ca one-third of body diam. at neck base. Lips amalgamated, labial and cephalic papillae scarcely projecting above lip contour. Amphidial fovea stirrup shaped, aperture slitlike, located at level of labial constriction, occupying 57- 67% of lip diam. Cheilostom cylindrical, with distinct indentation resembling an additional guiding ring, as described by Coomans (1985) in species of Californidorus and Enchodelus. Odontostyle 2.24-2.34 lip diam. long, well sclerotised with furcate base, 2.5-3.0 µ m in diam. or 13.6-15.3 times as long as broad, guiding ring double, located at 26-30 µ m or 1.3-1.6 lip diam. from anterior end. More or less spherical cavity with weakly sclerotised walls observed just posterior to guiding ring. Odontophore flanged, 1.0-1.2 times longer than odontostyle. Nerve ring located 130-143 µ m from anterior end. Pharynx composed of slender but muscular anterior portion and a muscular expanded part occupying 35.5-38.5% of total neck length, pharyngeal gland nuclei located as follows: DN = 69.0 ± 1.5 (67-72); S 1 N 1 and S 1 N 2 not observed; S 2 N 1 = 54.8 ± 1.0 (53-56); S 2 N 2 = 56.0 ± 1.9 (53-58). Very delicate membrane surrounding pharyngeal expansion. In some specimens, this structure apparently extending along ventral and/or dorsal side of pharyngeal expansion. Cardia hemispherical, about as broad as long, 12-13 × 11-13 µ m in size. Reproductive system amphididelphic, both branches equally developed. Ovaries reflexed, short, not reaching sphincter level, measuring 39- 63 µ m long, oocytes first in two or more rows, then in one row, distal part of ovary consisting of a well developed blind sac, similar to that described by Coomans (1964) in Discolaimus and Longidorus species. Oviduct joining ovary subterminally, close to proximal end of blind sac. Oviduct consisting of tubular part and well developed pars dilatata. Distinct sphincter visible between oviduct and uterus. Uterus 130-175 µ m or 1.9-2.5 body diam. long, tripartite, consisting of a distal spheroid pars dilatata, a slender intermediate portion and a wider proximal part, these three regions generally showing only weak development and obscured in some individuals by intestinal contents. Vagina extending inwards 51-60% of corresponding body diam. Pars proximalis vaginae 21.5 × 23.5 µ m, pars refringens composed of two trapezoidal, well sclerotised, pieces with a combined width of 16.5 × 4.0 µ m, pars distalis vaginae 6.3 µ m long (holotype). Sperm not observed in female genital tract. Prerectum 4.5-8.5 times and rectum 0.9-1.6 anal body diam. long. Tail short, rounded conoid, small saccate bodies observed along ventral surface, cuticle at terminus forming 42-57% of total tail length, two pairs of caudal pores located at middle of tail, one subdorsal, the other lateral. Male General morphology similar to that of female except for posterior region curved more ventrad. Reproductive system composed of two testes, anterior 179-262 and posterior 167-257 µ m long. Sperms ellipsoid, measuring 8.0-9.5 × 3.5 µ m. Spicules dorylaimoid, 1.3-1.8 anal diam. long and 3.9-4.8 times longer than wide, lateral accessory pieces paired, more or less cylindrical with bifurcate end, measuring 10.5-12.0×2.5 µ m. Series of 10-14 spaced ventromedian supplements starting 24- 32 µ m from adcloacal pair with posteriormost supplement located within range of retracted spicules, adcloacal pair located at 7.0-11.5 µ m from cloacal opening. Tail short conoid, dorsally convex, slightly ventrally concave with bluntly rounded terminus. Cuticle along ventral surface lacking saccate bodies. * The specific epithet is derived from Babak Fort, a historic location in the East Azarbaijan province of Iran and where the new species was found. M. Pedram et al. Juveniles Body length gradually increasing with stage. Comparison of length of functional odontostyle and replacement odontostyle identified four juvenile stages. Lip region in J1 flat, in J2 offset by a less marked constriction than in J3, J4 and adults. Lip region becoming progressively broader from J1 to J4. Genital primordium at vulval region observed in some J4. Tail in J1 conical, relatively much longer than in subsequent stages (cļ = 3), tail in J2- J4 similar in shape to that of female although becoming relatively shorter with stage (cļ = 1.0, 0.6-0.9 and 0.5-0.7 in J2, J3 and J4, respectively). TYPE MATERIAL Holotype female, ten paratype females, ten paratype males and paratype juveniles deposited in the Nematode Collection of the Faculty of Agriculture, University of Tabriz, Tabriz, Iran; two paratype females and two paratype males in separate slides deposited at CABI UK- Centre, Surrey, UK, and USDA Nematode Collection, Beltsville, MD, USA. TYPE HABITAT AND LOCALITY Rhizosphere of grasses from natural grasslands, Kaleibar City, mountains of Babak Fort, north-west Iran. DIAGNOSIS AND RELATIONSHIPS The new species is characterised by its 1.21-1.56 mm body length, lip region offset by marked constriction and 17.0-19.0 µ m broad, odontostyle 40-45 µ m long, odontophore flanged and 37-51 µ m long, neck region 283- 413 µ m long, pharyngeal expansion 100-136 µ m long or 35.5-38.5% of total neck length, amphididelphic female genital system, tripartite uterus, vulva pre-equatorial (V = 44-49), tail short, rounded (16-22 µ m long, c = 61- 93, cļ = 0.5- 0.7 in female; 19-30 µ m long, c = 49-72, cļ = 0.5- 0.9 in male), male as frequent as female with 49-61 µ m long spicules and 10-14 spaced ventromedian supplements, the posteriormost of which being within the range of the retracted spicules. Because of the morphology of the tail and the odontostyle length, E. babakicus n. sp. can be classified un- der the E. macrodorus -group as defined by Guerrero et al. (2008a). This group is characterised by the presence of a rather long odontostyle (> 35 µ m), odontophore with well developed flanges, tripartite uterus and rounded tail, and comprises the species described above together with E. distinctus Ahmad & Jairajpuri, 1980, E. groenlandicus (Ditlevsen, 1927) Thorne, 1939, E. macrodorus (de Man, 1880) Thorne, 1939, E. microdoroides Baqri & Jairajpuri, 1974 and E. saxifragae Popovici, 1995. In having a lip region offset by a strong constriction, the new species can be distinguished from E. groenlandicus, E. macrodorus and E. microdoroides. Furthermore, it can be separated from E. groenlandicus by its shorter female body (1.21-1.56 vs 1.57-2.50 mm), shorter odontostyle, odontophore and total stylet (40-45, 37-51 and 79-95 µ m, respectively, vs 44-53, 45-55 and 94-108 µ m), shorter and less developed uterus and frequent presence of males vs absence; from E. macrodorus, probably the closest relative, apart from the deep constriction at the lip region, the new species differs by its slightly more posteriorly located vulva (V = 44-49 vs 37-47), slightly longer uterus (130-175 vs 56-143 µ m or 1.9-2.5 vs 0.9-2.0 body diam.), female tail more conoid (vs almost hemispherical) and greater number of ventromedian supplements (10-14 vs 6-12); and from E. microdoroides by its slightly longer female body (1.21-1.56 vs 0.9-1.3 mm), broader lip region (17.0-19.0 vs 13.0-14.0 µ m), guiding ring located more anterior (26-30 vs 37-39 µ m from anterior end) and males with longer spicules (49-61 vs 45-50 µ m). The new species resembles the remaining two species, namely E. distinctus and E. saxifragae, in having the lip region offset by a deep constriction. However, it can be distinguished from E. distinctus by its shorter female body (1.21-1.56 vs 1.85 mm), longer odontostyle (40-45 vs 36 µ m), pre-equatorial vulva (V = 44-49 vs 53) and males frequent vs absent. Finally, the new species differs from E. saxifragae by the much shorter female body length (1.21-1.56 vs 1.80-2.38 mm), female genital system with less developed uterus, differently shaped pars refringens vaginae (trapezoidal vs arcuate drop-shaped), shorter tail (16-22 vs 22-40 µ m) and males with slightly shorter spicules (49-61 vs 56-70 µ m) and fewer ventromedian supplements (10-14 vs 13-16). In the key provided by Guerrero et al. (2008), E. babakicus n. sp. can either be keyed out together with E. microdoroides in step 1, due to the small size of some individuals, or with E. saxifragae in step 3. However, it can be easily distinguished from both by the characteristics listed above., Published as part of Pedram, Majid, Niknam, Gholamreza, Guerrero, Pablo, Ye, Weimin & Robbins, Robert T., 2009, Morphological and molecular characterisation of Enchodelus babakicus n. sp. and E. macrodorus Thorne, 1939 (Nematoda: Nordiidae) from Iran, pp. 895-907 in Nematology 11 (6) on pages 896-902, DOI: 10.1163/156854109X430563, http://zenodo.org/record/8114788
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- 2009
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30. Intraspecific Variability of Rotylenchulus reniformis from Cotton-growing Regions in the United States
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Agudelo, Paula, Robbins, Robert T., Stewart, James McD., and Szalanski, Allen L.
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Taxonomy and Systematics - Abstract
Reniform nematode (Rotylenchulus reniformis) is a major pest of cotton in the southeastern United States. The objective of this study was to examine the variation of reniform nematode populations from cotton-growing locations in the United States where it is prevalent. Multivariate analysis of variance and discriminant analysis were used to determine the variability of morphology in males and immature females. Reproduction indices of populations were measured on selected soybean and cotton genotypes in the greenhouse. High variability in morphometrics and reproduction was observed within all the populations, and several differences were found among populations. DNA sequences of the nuclear ribosomal first internal transcribed spacer region (ITS1) were compared among populations from the United States and to sequences of populations from Brazil, Colombia, Honduras, and Japan. No polymorphic nucleotide sites were observed among the amphimictic populations. Only a parthenogenic population from Japan was distinct. The phenotypic polymorphism of the species in the United States could impact the effectiveness of management strategies based on host plant resistance.
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- 2005
31. Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships
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Roshan-Bakhsh, Ali, primary, Roshan-Bakhsh, Ali, additional, Pourjam, Ebrahim, additional, Pedram, Majid, additional, Robbins, Robert T., additional, and Decraemer, Wilfrida, additional
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- 2014
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32. Axonchoides smokyensis sp. n. (Dorylaimida: Belondiridae) from the Great Smoky Mountains National Park: the second species of a very rare genus
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Peña-Santiago, Reyes, primary, Peña-Santiago, Reyes, additional, Abolafia, Joaquín, additional, Álvarez-Ortega, Sergio, additional, Ye, Weimin, additional, and Robbins, Robert T., additional
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- 2013
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33. IDENTIFICATION AND HAPLOTYPE DESIGNATION OF MELOIDOGYNE SPP. OF ARKANSAS USING MOLECULAR DIAGNOSTICS.
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Khanal, Churamani, Robbins, Robert T., Faske, Travis R., Szalanski, Allen L., McGawley, Edward C., and Overstreet, Charles
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ROOT-knot nematodes , *HAPLOTYPES , *MOLECULAR diagnosis , *MITOCHONDRIAL DNA , *SPECIES diversity - Abstract
In this study, polymerase chain reaction (PCR) and DNA sequencing analysis were performed to identify Meloidogyne species present in Arkansas. A total of 106 soil and root samples from 36 of the 75 counties were collected, of which 79 contained root-knot nematodes. To identify species, PCR was performed using primers C2F3/1108 to amplify a region of mitochondrial DNA (mtDNA) of root-knot nematodes. Additionally, M. incognita specific primers were designed to confirm speciation, as M. incognita was the most abundant species that was identified in 54 of the 79 samples. Other species found in this survey were M. marylandi, M. haplanaria, M. hapla, M. arenaria, and M. partityla. Haplotype designation was performed for each species based on nucleotide variation. With a limited number of samples, this study designated distinct mtDNA haplotypes of Meloidogyne spp. endemic in Arkansas. Unlike previous reports, M. javanica and M. graminis were not detected from any of the samples collected during this study. [ABSTRACT FROM AUTHOR]
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- 2016
34. FIRST REPORT OF MELOIDOGYNE PARTITYLA PARASITIZING PECAN IN ARKANSAS AND CONFIRMATION OF QUERCUS STELLATE AS A HOST.
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Khanal, Churamani, Szalanski, Allen L., and Robbins, Robert T.
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ROOT-knot nematodes ,PECAN ,OAK diseases & pests ,NEMATODE hosts ,DISEASES - Abstract
Copyright of Nematropica is the property of Organization of Nematologists of Tropical America and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2016
35. Morphological and molecular characterisation of Xiphinema mazandaranense n. sp. (Dorylaimida: Longidoridae), a new member of the Xiphinema pyrenaicum species complex
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Pedram, Majid, primary, Pourjam, Ebrahim, additional, Robbins, Robert T., additional, Ye, Weimin, additional, Atighi, Mohammad Reza, additional, and Decraemer, Wilfrida, additional
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- 2012
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36. Morphological and molecular characterisation of Xiphinema zagrosense sp. n. (Dorylaimida: Longidoridae) from the Zagros Mountains, Iran
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Ghaemi, Razieh, primary, Pourjam, Ebrahim, additional, Pedram, Majid, additional, Robbins, Robert T., additional, Ye, Weimin, additional, and Decraemer, Wilfrida, additional
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- 2012
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37. Description of Rhyssocolpus vinciguerrae sp. n. (Dorylaimida, Nordiidae) from Iran and the first molecular study of this genus
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Peña-Santiago, Reyes, primary, Ye, Weimin, additional, Robbins, Robert T., additional, Pourjam, Ebrahim, additional, and Pedram, Majid, additional
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- 2011
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38. Registration of S99-2281 Soybean Germplasm Line with Resistance to Frogeye Leaf Spot and Three Nematode Species
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Shannon, J. Grover, primary, Lee, Jeong-Dong, additional, Wrather, J. Allen, additional, Sleper, David A., additional, Mian, M. A. Rouf, additional, Bond, Jason P., additional, and Robbins, Robert T., additional
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- 2009
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39. Morphological and molecular characterisation of Enchodelus babakicus n. sp. and E. macrodorus Thorne, 1939 (Nematoda: Nordiidae) from Iran
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Pedram, Majid, primary, Niknam, Gholamreza, additional, Guerrero, Pablo, additional, Ye, Weimin, additional, and Robbins, Robert T., additional
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- 2009
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40. RENIFORM NEMATODE REPRODUCTION ON SOYBEAN CULTIVARS AND BREEDING LINES IN 2005 TESTS.
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Robbins, Robert T., Rakes, Larry, Jackson, Larry E., Gbur, E. E., Dombek, Donald G., Chen, P., Shipe, E., and Shannon, Grover
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During 2005, 209 soybean varieties from the Arkansas variety testing program and 52 breeding lines (24 breeding lines from the Clemson, 15 from the Arkansas, and 13 from the Missouri breeding programs) were tested in the greenhouse to determine their suitability as hosts for the reniform nematode (RN), Rotylenchulus reniformis. All treatments were inoculated with 1,056 vermiform RN. The RN resistant varieties Anand, Forrest, and Hartwig, the RN susceptible cultivar Braxton, and fallow RN-infested soil served as controls. In all 2005 tests Forrest was not uniform in RN resistance. The mean number of vermiform nematodes extracted from the soil of each treatment was calculated, as were the reproductive indices (RI = Pf/Pi), and PF/PI of Hartwig (Arkansas test cultivars) or Anand (breeding lines). Arkansas test cultivars with RI's significantly greater than the RI on Hartwig (1.00) were considered suitable hosts for R. reniformis. Of the Arkansas test varieties 207 of 209 had more RN reproduction than Hartwig. The following varieties, Ax RR53116 and SO 2-3934RR, were not different than Hartwig. On 38 of the 52 breeding lines RN reproduced more than on Anand. Of the 13 breeding lines from Missouri, seven were not different than Anand (S01-9265, S02-3934, S01-9391, S02-611CR, S019364, S01-8401(HP), S02-20388 ), of the 24 from Clemson 5 were not different than Anand (SC02-212, SC02-210, SC01-819, SC02-208, SC02-211 )and of the 15 breeding lines from Arkansas (R97-1801, R001551 ) seven were not different than Anand. [ABSTRACT FROM AUTHOR]
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- 2006
41. MOLECULAR BASED-PHYLOGENETIC RELATIONSHIPS IN THE SUPERFAMILY CRICONEMATOIDEA USING ITS1-rDNA.
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Cordero, Marco A., Robbins, Robert T., and Szalanski, Allen L.
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NEMATODE morphology , *MOLECULAR phylogeny , *RIBOSOMAL DNA , *BAYESIAN analysis , *GENETIC markers - Abstract
Populations of nematodes of the superfamily Criconematoidea were obtained and identified morphologically from different geographical areas in the continental United States. This previous information was used to analyze their phylogenetic relationship based on the first internal transcribed spacer ribosomal region. Phylogenetic analysis of the ITS1-rDNA region showed monophyletic and paraphyletic groups in Criconematidae: A) species of Mesocriconema and Criconemoides; and B) Bakernema, Criconema Hemicriconemoides and Xenocriconemella species. Subfamily Hemicycliophorinae, (Caloosia, Hemicaloosia and Hemicycliophora) and family Tylenchulidae (Paratylenchus and Gracilacus) were not resolved because of the high variation of the marker Molecular phylogenetic analysis using the ITS1-rDNA marker rejects the hypothesis of a common ancestor for criconematids with double sheath or cuticle among others characters. This molecular phylogenetic study showed that ITS1-rDNA sequences are too variable to study phylogenetics of Criconematoidea. However, ITS1-rDNA is useful to identify and to characterize species within families. [ABSTRACT FROM AUTHOR]
- Published
- 2013
42. Plant-Parasitic Nematodes: A Pictorial Key to Genera.William F. Mai , Peter G. Mullin
- Author
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Robbins, Robert T., primary
- Published
- 1996
- Full Text
- View/download PDF
43. Plant-Parasitic Nematodes: A Pictorial Key to Genera, 5th ed
- Author
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Robbins, Robert T.
- Subjects
Plant-Parasitic Nematodes: A Pictorial Key to Genera, 5th Ed (Book) -- Book reviews ,Books -- Book reviews - Published
- 1996
44. Description of Rhyssocolpus vinciguerrae sp. n. (Dorylaimida, Nordiidae) from Iran and the first molecular study of this genus.
- Author
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Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin, and Peña-Santiago, Reyes
- Subjects
HABITATS ,ENCHODELUS ,PLANT morphology ,MORPHOMETRICS ,RECOMBINANT DNA - Abstract
Rhyssocolpus vinciguerrae sp. n., from natural habitats in Iran, is described, illustrated and sequenced. It is characterised by the body length of 1.04-1.37 mm, lip region offset by depression and 11-13 μm broad, odontostyle 8-10 μm or 0.7-0.8 times the lip region diam. long, neck 227-265 μm long, pharyngeal expansion 80-95 μm long or 31-35% of total neck length, a dorsal cell mass present between cardia and the end of the anterior ovary/testis, uterus bipartite, 90-170 μm long, vulva longitudinal (V = 48-55), abundant irregularities in body cuticle around vulva, female tail conical with rounded terminus (35-55 μm, c = 24-34, c′ = 1.4-1.9), spicules 43-51 μm long and 6-8 spaced ventromedian supplements located outside the range of the spicules. It is very similar to R. aquilonius, R. arcticus and R. iuventutis. A molecular characterisation of the new species was done on ribosomal DNA nearfull-length small subunit 18S gene, internal transcribed spacer and partial 5.8S gene. The results obtained support a close relationship between Rhyssocolpus and Heterodorus, and a more distant relationship with Enchodelus. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
45. Description of one new, and new data on two known, species of Enchodelus Thorne, 1939 (Dorylaimida: Nordiidae) from Iran.
- Author
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Pedram, Majid, Pourjam, Ebrahim, Robbins, Robert T., Ye, Weimin, and Peña-Santiago, Reyes
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ENCHODELUS ,SPECIES ,PHYLOGENY ,ANIMAL morphology - Abstract
This contribution provides a study of one new and two known species of Enchodelus. Enchodelus sardashtensis sp. n. is characterised by its 1.3-1.5 mm long body, lip region 13-15 μm broad and offset by marked depression, odontostyle 22-27 μm or 1.5-1.8 lip region diam. long, neck 290-310 μm long, pharyngeal expansion 100-110 μm long or 35-38% of total neck length, a dorsal cellular mass (pseudocoelomocyte) present between cardia and proximal end of anterior ovary, uterus tripartite and 95-145 μm long, V = 44-48, female tail rounded conoid (19-23 μm, c = 61-76, c′ = 0.7-0.8) and bearing saccate bodies, and male unknown. This species is close to E. altherri and E. arcticus. Enchodelus groenlandicus and E. longispiculus are recorded for the first time in Iran and in Asia, respectively and compared to other known populations of these species. A molecular characterisation of E. longispiculus is also performed based on ribosomal DNA near-full-length small subunit 18S gene, internal transcribed spacer and partial 5.8S gene. Phylogenetic analysis revealed E. sardahtensis sp. n. and E. longispiculus to be in a monophyletic clade with all other Enchodelus with rounded tail. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
46. Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis.
- Author
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BAE, Chang-Hwan, ROBBINS, Robert T., and SZALANSKI, Allen L.
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NEMATODES , *POLYMERASE chain reaction , *RECOMBINANT DNA , *ENZYMES , *DNA primers - Abstract
Two different molecular approaches, a multiplex PCR and PCR-RFLP of ITS-rDNA, were developed for the identification of Hoplolaimus species. DNA sequences of H. columbus, H. galeatus, H. concaudajuvencus, H. magnistylus, H. seinhorsti and three undescribed Hoplolaimus species were used to design species-specific primers. Three reverse species-specific PCR primers for H. columbus, H. galeatus and H. magnistylus were developed using the ITS1 region exhibiting interspecific variation. Three species-specific PCR primers in combination with the forward primer, Hoc-1f, produced distinct amplicons of 580 bp for H. columbus, 120 bp for H. galeatus and 340 bp for H. magnistylus. We successfully identified each of three species by multiplex PCR when all three were mixed in a single PCR reaction. Restriction enzyme digests of the PCR amplicon using HaeIII and RsaI permitted discrimination of H. columbus, H. galeatus, H. magnistylus, H. concaudajuvencus, H. sp. 1, H. sp. 2 and H. sp. 3 from each other. These results suggest that these molecular techniques allow for rapid, easy and reliable identification of Hoplolaimus species. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
47. Additional data on Enchodelus veletensis Guerrero, Liébanas & Peña-Santiago, 2007 (Dorylaimida: Nordiidae) from Iran, and phylogeny of the genus.
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PEDRAM, Majid, NIKNAM, Gholamreza, GUERRERO, Pablo, Weimin YE, and ROBBINS, Robert T.
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ENCHODELUS ,ANIMAL species ,GRASSES ,ANIMAL sexual behavior ,PHYLOGENY - Abstract
Additional information on the recently described species, Enchodelus veletensis, is provided, with females, males, intersex and juveniles being described from a population recovered from soil samples collected about grasses in a natural region of north-western Iran. This is the first report of this species for male, intersex and juveniles. Nearly full length of 1560 bp 18S rDNA and 828 bp ITS1 were sequenced. Phylogenetic analysis based on 18S rDNA supported two distinct monophyletic groups of Enchodelus species, those with rounded tail vs conical tail. Enchodelus veletensis is closely related to E. brevidentatus and E. arcuatus. It has identical sequences with E. brevidentatus in 638 bp partial 18S rDNA yet is morphologically distinct. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
48. Xiphinema iranicum n. sp. (Nematoda: Longidoridae) from north-western Iran.
- Author
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PEDRAM, Majid, NIKNAM, Gholamreza, ROBBINS, Robert T., YE, Weimin, and KAREGAR, Akbar
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DAGGER nematodes ,RHIZOSPHERE ,MOLECULAR phylogeny ,SPECIES - Abstract
Xiphinema iranicum n. sp. is described from soil samples collected about the rhizosphere of Rosa sp. growing in a natural mountainous region close to Maragheh city, north-western Iran. The new species belongs to group 6 of the polytomous key to species identification and is unique by having a medium body length (3.8-4.3 mm), odontostyle length of 141-162 μm, odontophore length of 90-98 μm, mid-body diam. of 69-88 μm, presence of spines in the uterus and a hemispherical to conoid tail with a rounded terminus or minute bulge. It resembles X. ingens, X. aceri, X. aequum, X. smoliki, X. illyricum, X. macedonicum and X. vuittenezi but differs from X. aceri and X. aequum by having different tail shape, longer odontophore, lower ratio a, higher ratio c and lower ratio c′. Compared to X. ingens and X. smoliki, it differs by having lower ratio a, lower ratio b, spines in uterus vs crystalline bodies plus pseudo Z-organ in X. ingens and spines plus pseudo Z-organ in X. smoliki and also by the presence of rare males, and, finally, it can be distinguished from X. vuittenezi by body and odontostyle length, characters of juveniles and molecular differences. The new species has similar polytomous identification codes to X. illyricum and X. macedonicum but can be differentiated from both by longer body, higher ratio c, higher V, longer odontostyle and odontophore and larger body diam. at mid-body and anus level. Ribosomal gene 18S rDNA and ITS1 of X. iranicum n. sp., X. aceri, X. montenegrinum and X. macedonicum, all obtained from north-western Iran in the same survey, were sequenced to investigate the phylogenetic relationships with other sequenced Xiphinema species. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
49. SSR Mapping and Confirmation of Soybean QTL from P1 437654 Conditioning Resistance to Reniform Nematode.
- Author
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Bo-Keun Ha, Robbins, Robert T., Feng Han, Hussey, Richard S., Soper, John F., and Boerma, H. Roger
- Subjects
- *
NEMATODES , *WORMS , *SOYBEAN , *CULTIVARS , *RESTRICTION fragment length polymorphisms , *DNA fingerprinting , *FORAGE plants , *AGRICULTURE - Abstract
Recently, reniform nematode (Rotylenchulus reniformis Linford and Oliveira) has been recognized to be a major problem on soybean [Glycine max (L.) Merr.] in the southern USA. Planting reniform nematode—resistant soybean cultivars is the most cost effective control method. With restriction fragment length polymorphism (RFLP) markers, Pioneer Hi-Bred International, Inc. had previously identified quantitative trait loci (QTL) on linkage group B1 (LG-B1) and another on LG-L associated with reniform nematode resistance in a cross between ‘BSR101’ and PI 437654. In this study, we refined the QTL locations and identified additional QTL conditioning resistance to reniform nematode in a population of 228 recombinant inbred lines (RILs) from a cross of BSR101 × PI 437654. A QTL (R2 = 21%) conditioning reniform reproductive index (RI) was found on LG-L and was flanked by Sat_184 and Satt513. Two other QTL were identified, one on LG-B1 (R2 = 16%) and the other on LG-G (R2 = 8%). These two QTL acted in an epistatic manner with lines homozygous for PI 437654 alleles at both QTL providing the lowest reniform RI. The 31 RILs from BSR101 × PI 437654 that were homozygous for the PI 437654 alleles at Satt513, Satt359, and Sat_168 averaged 1077 reniform eggs and juveniles (RI = 0.63), while the 33 RILs homozygous for the BSR101 alleles averaged 10606 (RI = 6.24). By screening the RILs population of ‘Prichard’ × ‘Anand’ we confirmed that the QTL on LG-G and-B1 conditioned reniform RI. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
50. SOD polymorphism in the Xiphinema americanum-group (Nematoda: Longidoridae).
- Author
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Molinari, Sergio, Lamberti, Franco, Duncan, Larry W., Halbrendt, John, McKenry, Michael, Abawi, George S., Magunacelaya, Juan Carlos, Crozzoli, Renato, Lemos, Rui M., Nyczepir, Andrew P., Nagy, Peter, Robbins, Robert T., Kotcon, James, Moens, Maurice, and Brown, Derek J. F.
- Subjects
SUPEROXIDE dismutase ,WORMS ,ANIMAL populations ,DAGGER nematodes ,ENZYMES ,PHENOTYPES - Abstract
Isoelectrofocusing of superoxide dismutase (SOD) isoforms was carried out on the extracts of 117 nematode populations belonging to the so-called Xiphinema americanum-group. These populations came from the USA (77), Chile (5), Argentina (1), Venezuela (5), Portugal (15), Italy (2), Crete (1), Montenegro (1), Slovakia (4), Hungary (3), Egypt (1) and India (2). A total of 17 bands of enzyme activity were observed in the screening, whilst single enzyme phenotypes showed from two to eight bands. The high degree of SOD polymorphism of this nematode collection was grouped by cluster analysis into seven distinct homogeneous groups characterised by specific combinations of SOD markers. Sub-groups could be discriminated for larger groups. The small Groups 3 and 5 were constituted mostly by populations from USA east coast states (i.e., NY and PA, respectively). The larger Group 1 resulted from the association of populations coming from various and distant North American States. In other large groups, North American populations were associated with South American and European populations. Overall, the data presented here suggest that geographic separation and different hosts do not seem to be the source of genetic diversity for the X. americanum-group. When an adequate number of populations were collected from the same country, the variability expressed by such a sub-sample was comparable to that of the whole nematode collection. For the first time, homogeneous populations of a large collection of X. americanum-group populations were associated by molecular means in order to explore further approaches that may help resolve the recalcitrant taxonomy and phylogeny of this much debated group. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
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