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AbstractRosa chinensis var. spontanea, an important horticultural species, holds high ornamental value. To investigate the phylogenetic relationships and organelle genome data of R. chinensis var. spontanea and its related species, the organelle genomes were constructed and sequenced in this study. The complete mitochondrial genome consists of a linear DNA molecule with a length of 270,143 base pairs and contains 55 genes. The whole chloroplast genome harbors 131 annotated genes. There are 39 and 54 simple sequence repeats (SSRs) in the mitochondrial and chloroplast genomes of R. chinensis var. spontanea, respectively. The phylogenetic tree of the mitochondrial genome indicates that R. chinensis var. spontanea is related to R. chinensis. The codon usage biases of one mitochondrial gene and nine chloroplast genes in Rosaceae were affected by mutation, while those of other genes have undergone selection. Overall, our findings suggest that the information within the organelle genomes can be utilized for constructing molecular markers, conducting genetic evolution research, and interpreting the phylogenetics of plants. The results of this study will contribute to a more in - depth investigation of the population genetics and evolution of Rosaceae. [ABSTRACT FROM AUTHOR]

With their high economic value and cultural significance, modern roses are one of the most important ornamental plants. Because of their complicated genetic background and tetraploid nature, the creation of high-density genetic maps of roses has been a challenge that has slowed the pace of molecular breeding for modern roses. The current construction of tetraploid genetic maps based on existing diploid rose genomes could lead to inaccurate marker information and genotyping results. Therefore, we generated the first high-quality tetraploid genome of Rosa chinensis 'Yunzheng Xiawei.' Utilizing Illumina, PacBio, and Hi-C sequencing technologies, we assembled a genome of 858.59 Mb with 14 pseudo-chromosomes. Mode of inheritance analysis using PolyOrigin indicated that modern roses show both quadrivalent and bivalent pairing. Based on this reference genome, high-density genetic maps were constructed using MSTmap with nearly saturated markers. Quantitative trait locus (QTL) analysis was conducted using WinQTLCart and R/qtl for flavonoids and carotenoids, and 11 QTL clusters were identified. By combining the genome annotation, phylogenetic analyses, and gene expression analyses, we were able to identify several key genes related to flavonoid and carotenoid biosynthesis. This study provides the basis for further genetic analyses of highly heterozygous tetraploid roses and could facilitate the progress of marker-assisted selection in modern roses. [ABSTRACT FROM AUTHOR]

Purpose: Robotic‐assisted total knee arthroplasty (RA‐TKA) is an increasingly popular alternative that may increase the accuracy of conventional TKA techniques. This study aims to evaluate RA‐TKA accuracy and compare its radiographic and clinical outcomes to conventional TKA (cTKA). Methods: A retrospective examination of patients with bi‐ or tricompartmental knee osteoarthritis who underwent RA‐TKA (RObotic Surgical Assistant system) or cTKA and were prospectively documented in the TKA registry. Accuracy was assessed using standardized radiographic implant position evaluations, namely femoral and tibial coronal angles and femoral and tibial sagittal angles. Baseline demographics, surgery details and 6‐ and 12‐month post‐TKA patient‐reported outcomes (PROMs; e.g., Oxford Knee Score [OKS] and Core Outcome Measures Index) were compared between RA‐TKA and propensity score‐matched cTKA patients. Results: Overall correlation between preset and 6‐week postoperative angle measurements for RA‐TKA was low with significant differences noted only for mean tibial sagittal angles (84.6° [RA‐TKA] vs. 82.3° [cTKA]) (p < 0.001). The study groups were demographically similar, although RA‐TKA patients had slightly longer operative times and higher blood loss but shorter inpatient stays. There were sustainable improvements in all PROMs already at 6 months, yet RA‐TKA patients had significantly higher OKS values over their conventional counterparts at this time point. Conclusion: Radiological and clinical outcomes were comparable between RA‐TKA and cTKA. The robotic‐assisted system demonstrated higher accuracy in the coronal than sagittal plane and RA‐TKA patients achieved better short‐term outcomes for pain and disability. While both methods are similar in the hands of a skilled surgeon, long‐term studies are necessary to establish clear method superiority. Level of Evidence: Level III, therapeutic study. [ABSTRACT FROM AUTHOR]

Three types of Rosa canina plant extracts were prepared. The antioxidant activity of the first prepared solution was measured using a UV spectrophotometer in the bioengineering laboratory. The resulting graph shows that the DPPH free radical is blocked by the antiradical antioxidant contained in the rosehip plant extract. We then analyzed the resulting graph using Origin Corporation software and obtained a new graph. Origin Corporation software offers a wide range of features, making it easy to analyze the laboratory results. The software analyzes the results by performing mathematical calculations and provides more detailed information. These analyses allow for the evaluation of the potential antioxidant properties of the Rosa canina plant and enhance the understanding of its potential use in medical fields. The findings could contribute to the future application of rosehip in therapeutic practices and health-related research. [ABSTRACT FROM AUTHOR]

Copyright of Gerokomos is the property of Indemm Farma SL and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Interspecific hybridization in the genus Rosa (Rosaceae) is a common natural phenomenon. Hybrids often exhibit heterosis and new combinations of traits, which can provide raw materials for horticultural breeding. DNA barcodes and microsatellites have been proposed to facilitate species discrimination and hybrid detection. However, most SSR markers developed for roses have been found unapplicable to Rosa sect. Synstylae because of null alleles or failed amplification. In this study, we designed 15 pairs of microsatellite primers, along with four previously developed primers specifically for Rosa sect. Synstylae; we then analyzed 174 individuals of three closely related and sympatrically distributed Rosa species as a test case to evaluate the consistency between morphological and genetic hybrid identifications and to compare the discrimination efficiency of the DNA barcodes versus SSRs in detecting admixture. Principle coordinate analysis identified several individuals with intermediate phenotypes among the three rose species. Hybridization, intraspecific morphological polymorphism, and sample collection at different growth stages or phenological phases may have hindered species identification based on morphology and distorted the morphological clustering results. The molecular analyses showed that 12 (6.8%), 13 (7.4%), and 15 (8.6%) individuals were identified as admixed by STRUCTURE, NewHybrids, and nrITS sequences, respectively, of which only seven hybrids showed signs of admixture across all three methods. About 81% of the morphologically identifiable hybrids exhibited admixture based on SSRs. Meanwhile, approximately 69% of morphologically identifiable hybrids were detected, but four morphologically pure species individuals were identified as genetically admixed based on nrITS sequences. Some morphologically pure species individuals were genetically identified as hybrids while some morphological hybrids were identified as pure individuals based on certain molecular markers. Overall, EST-SSRs discriminated morphological hybrids more accurately than nrITS. We inferred that there is ongoing interspecific gene exchange among the three wild Rosa species that obscures morphospecies boundaries. Combining multiple data types and analytical approaches offers powerful utility for hybrid detection, regardless of the level of hybridization. [ABSTRACT FROM AUTHOR]

El propósito de este texto es reflexionar sobre el libro El Nombre de la Rosa. Esta poesía intelectual nos permite analizar la evolución de la percepción de los Universales a lo largo de la historia. Desde el título hasta su final, el autor nos lleva en un camino lleno de cambios históricos y autopercibidos. Es interesante cómo el contexto determinante siempre juega un papel relevante y el poder de las ideas trasciende muchas veces la realidad que uno experimenta. En este sentido, Umberto Eco, nos muestra como diferentes aspectos filosóficos pueden implementarse sin tener que elegir uno por sobre otro, lo que nos lleva a meditar sobre la utopía en la simbiosis filosófica. Este enfoque permite ver la relación entre los aspectos ontológicos, gnoseológico, lingüísticos y otros campos, como la medicina. En los siguientes párrafos, exploraré algunos de estos aspectos y cómo se entrelazan con nuestra disciplina. La filosofía nos permite analizar y comprender los conceptos abstractos que subyacen a la práctica médica; y puede ayudarnos a mejorar la calidad de atención que brindamos a nuestros pacientes, como a nuestra relación con colegas.

Introduction: Prickles are often deemed undesirable traits in many crops, including roses (Rosa sp.), and there is demand for rose cultivars with no or very few prickles. This study aims to identify new and/or validate reported quantitative trait loci (QTLs) associated with stem and leaf rachis prickle density, characterize the effects of functional haplotypes for major QTLs, and identify the sources of QTL-alleles associated with increased/decreased prickle density in roses. Methods: QTL mapping using pedigree-based analysis (PBA), and haplotype analysis were conducted on two multi-parental diploid rose populations (TX2WOB and TX2WSE). Results and discussion: Twelve QTLs were identified on linkage groups (LGs) 2, 3, 4, and 6. The major QTLs for the stem prickle density were located between 42.25 and 45.66 Mbp on chromosome 3 of the Rosa chinensis genome assembly, with individual QTLs explaining 18 to 49% of phenotypic variance (PVE). The remaining mapped QTLs were minor. As for the rachis prickle density, several QTLs were detected on LG3, 4, and 6 with PVE 8 to 17%. Also, this study identified that ancestors R. wichurana 'Basye's Thornless', 'Old Blush', and the pollen parent of M4-4 were common sources of favorable alleles (q) associated with decreased prickle density, whereas 'Little Chief' and 'Srche Europy' were the source of unfavorable alleles (Q) in the TX2WOB and TX2WSE populations, respectively. The outcomes of this work complement other studies to locate factors that affect prickle density. These results can also be utilized to develop high-throughput DNA tests and apply parental selection to develop prickle-free rose cultivars. [ABSTRACT FROM AUTHOR]

Copyright of Cuadernos del Centro de Estudios de Diseño y Comunicación is the property of Cuadernos del Centro de Estudios de Diseno y Comunicacion and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The Enemy Release Hypothesis predicts that introduced species in their new range are freed from natural enemies (e.g., pathogens, parasitoids and predators) that control their populations.Diplolepis rosae (Hymenoptera, Diplolepididae), native to the Western Palearctic, induces readily apparent galls on wild roses (Rosa spp.) that support a robust component community of inquilines, parasitoids and hyperparasitoids in its native range. D. rosae was introduced to North America in the mid‐1800s, and has since become widespread and common across the continent.We compared the insect communities associated with D. rosae galls from Canada and the US Pacific Northwest with those of Eastern Europe.Throughout its introduced range, parasitism rates were lower compared with galls in their natural range. Component communities were also less diverse and species‐rich. The relationship between gall size and parasitism rates showed no significant difference between the two continents.These results show that the component community in its introduced range is depauperate and provide support for the Enemy Release Hypothesis. [ABSTRACT FROM AUTHOR]

The genus Rosa includes more than 200 species and 18 000 cultivars, which are used in floriculture, and they are also economically important due to the presence of essential oil, a source of natural aromatic components. Products (essential oil, rose oil, rose water, etc.) obtained from essential-bearing oil roses are used in the food, perfume, cosmetic, and medical industries. Traditional rose propagation is carried out by cuttings, grafting, or budding, which are complex and time-consuming processes. Biotechnological methods have become an alternative to the vegetative method of rose propagation. The main studies are aimed at selecting the mineral composition of culture media and concentrations of growth regulators, while the issues of structural and genetic stability of the material in vitro are still discussed. Therefore, the object of our work was to determine the optimal conditions for in vitro cultivation, structure, ploidy, and relative DNA content of essential oil rose "Festivalnaya" microshoots. It was shown that the Murashige and Skoog culture medium containing 1.0 mg/L 6-benzylaminopurine and 1.0 mg/L kinetin was effective for the propagation of the material. Structural analysis of microshoots after cultivation for 12 months showed qualitative and quantitative changes caused by specific in vitro conditions and rejuvenation of the material. The ploidy of nuclei in leaf cells in vitro did not differ from that of shoots ex situ as did the relative DNA content (2.21 and 2.24 pg, respectively), indicating a certain stability of the material in vitro. [ABSTRACT FROM AUTHOR]

Abstract Background One of the most widely used medicinal plants in Iranian traditional medicine, Rosa × damascena Herrm. (mohammadi flower) that the people of Kashan use as a sedative and to treat nervous diseases and constipation. In this research, the yield, chemical composition and antimicrobial activity of the essential oil of this plant were evaluated for the first time from Azaran region, Kashan. Methods The essential oil was extracted by means of hydrodistillation (Clevenger), and its chemical compounds were identified and determined by GC/MS. The antimicrobial activity of the essential oil was determined by the diffusion method in agar, the minimum growth inhibitory concentration (MIC) and the minimum concentration capable of killing bacterial/fungal microorganisms (MBC/MFC). Results The results showed that the yield of essential oil was 0.1586 ± 0.0331% (w/w). Based on the results of the chemical composition analysis of R. x damascena essential oil, 19 different compounds (98.96%) were identified. The dominant and main components of the essential oil were oleic acid (48.08%), palmitic acid (15.44%), stearic acid (10.17%), citronellol (7.37%) and nonadecane (3.70%). Based on the results of diffusion in agar, the highest zone of inhibition against Candida albicans (ATCC 10231) was ~ 9.5 mm. The strongest inhibitory activity of R. x damascena essential oil against Gram-negative Proteus mirabilis (ATCC 43071) was with the diameter of the inhibition zone (~ 9 mm), which was equal to the strength of rifampin (~ 9 mm). Conclusion Therefore, this essential oil is a promising natural option rich in fatty acids, which can be a potential for the production of natural antimicrobials against infectious diseases, especially urinary tract infections.

Four species of the genus Phragmidium parasitizing plants of the genus Rosa (Rosaceae) are recorded in Ukraine. Phragmidium fusiforme occurs only in the Carpathians. Phragmidium rosae-pimpinellifoliae was reported only from the Crimean Peninsula. Phragmidium mucronatum and P. tuberculatum are distributed much more widely and occur in Ukraine much more frequently. A reliable feature for distinguishing these two species is a character of the upper cell and papilla of teliospores: in P. mucronatum it attenuates gradually toward the apex to form a papilla, while in P. tuberculatum the papilla is clearly delimited from the rounded apex of the upper cell of the teliospore. An interesting feature of all three species illustrated in this article but not reported previously in mycological literature is a twisting of the upper part of the pedicel of their teliospores. The article is illustrated with photomicrographs obtained using a scanning electron microscope.

Agrilus cuprescens (Ménétries, 1832), the rose stem girdler, is a Palearctic Buprestidae (Coleoptera) pest of Rosa and Rubus species. Its introduction and consequent spread across North America may now make it the most widely distributed Agrilus species, globally.No evidence reviewed indicated A. cuprescens was present in North America long before it was first reported on Rosa in New Jersey, USA, in 1913. The species incrementally progressed westward, reaching the Pacific coast in Washington, USA, 101 years later. Future spread is most likely within the northern Canadian prairies and the Pacific Northwest regions. Its presence in the latter region poses a contemporary threat to epicentres of Rubus production in both the United States and Canada. As of 2022, confirmed spread within the region extended nearly to the Canadian border in Washington and the southern Willamette Valley in Oregon.The most comprehensive, foundational investigations of A. cuprescens in North America were conducted in the late 1930s and early 1940s in New York, USA, and in Utah, USA, during the late 1950s and early 1960s following damaging outbreaks on Rubus. Ongoing research in the Pacific Northwest is attempting to refine information on this pest as it progresses towards naturalization within a new North American ecological and agricultural context.This review aggregates and clarifies the geographical progression, biological understandings and management of this species in North America, which has been fraught with a convoluted history of misidentifications, and varying degrees of consideration given to it as a pest of concern. [ABSTRACT FROM AUTHOR]

This study investigates the micromorphology and morphometrics of aggregate fruit (ripe hypanthium and achene) of eight wild Rosa species collected throughout Serbia. Field research was conducted to find roses at different habitats, collecting 1,836 aggregate fruits with 25,999 achenes from 137 shrubs. Multicellular glandular trichomes are found on the hypanthium surface, while long and short stalk capitate glandular trichomes were observed on the fruits of R. gallica, R. pendulina, R. tomentosa and R. villosa. Numerous unicellular non-glandular trichomes were found inside the urceolate hypanthium and on the surface of most studied achenes, except for R. spinosissima species. Micromorphological analysis revealed three types of exocarp ornamentation: scalariform (R. dumetorum, R. tomentosa and R. micrantha), reticulate (R. gallica, R. arvensis, R. spinosissima and R. villosa) and reticulate-smooth (R. pendulina). Morphometric analysis indicated significant differences among the 14 morphological characters. Achene length was the most significant morphometric feature for identifying Rosa species (P˂0.05). The results contribute to our poor knowledge of rose fruit morphology and provide some well-defined microcharacters that could be used as additional features for species delimitation. [ABSTRACT FROM AUTHOR]

Most wild rose species in the Netherlands belong to Rosa section Caninae (dogroses), with Rosa arvensis (section Synstylae) and Rosa spinosissima (section Pimpinellifoliae) as other indigenous species. All species are rare, often found in small populations or as scattered individuals, except for Rosa canina and Rosa corymbifera. Conservation strategies have been developed for these roses, with a focus on ex situ methods, including clonal archives and seed orchards, using vegetative propagation from the original shrubs. Efficient collection management aims at preservation of maximum genetic diversity with a minimum of duplicated genotypes. However, dogrose taxonomy is complex because of species hybridization, different ploidy levels, and their matroclinal inheritance due to Canina meiosis. They can also reproduce vegetatively through root suckers. In order to assess the genetic structure and the levels of genetic diversity and clonality within and among the wild rose populations in the Netherlands, we genotyped individuals in wild populations and accessions in the ex situ gene bank with 10 highly polymorphic microsatellite markers. The analysis revealed 337 distinct multilocus genotypes (MLGs) from 511 sampled individuals, with some MLGs shared across different species and sites. The genetic structure analysis showed distinct clusters separating non-dogrose species from the Caninae section. Geographic distribution of MLGs indicated both local and widespread occurrences. Redundancy analysis identified 152 distinct MLGs from 244 gene bank accessions, suggesting a 38% redundancy rate. Core collections were optimized to retain genetic diversity with minimal redundancy, selecting subsets of 20–40 individuals from different species groups. The study highlights the value of genetic characterization in guiding sampling strategies for dogroses. We propose a two-step approach that may be used to reveal clonality and redundancy and to optimize core collections of species that combine sexual and vegetative reproduction, to maximize genetic capture in ex situ gene banks. [ABSTRACT FROM AUTHOR]

Background: One of the most widely used medicinal plants in Iranian traditional medicine, Rosa × damascena Herrm. (mohammadi flower) that the people of Kashan use as a sedative and to treat nervous diseases and constipation. In this research, the yield, chemical composition and antimicrobial activity of the essential oil of this plant were evaluated for the first time from Azaran region, Kashan. Methods: The essential oil was extracted by means of hydrodistillation (Clevenger), and its chemical compounds were identified and determined by GC/MS. The antimicrobial activity of the essential oil was determined by the diffusion method in agar, the minimum growth inhibitory concentration (MIC) and the minimum concentration capable of killing bacterial/fungal microorganisms (MBC/MFC). Results: The results showed that the yield of essential oil was 0.1586 ± 0.0331% (w/w). Based on the results of the chemical composition analysis of R. x damascena essential oil, 19 different compounds (98.96%) were identified. The dominant and main components of the essential oil were oleic acid (48.08%), palmitic acid (15.44%), stearic acid (10.17%), citronellol (7.37%) and nonadecane (3.70%). Based on the results of diffusion in agar, the highest zone of inhibition against Candida albicans (ATCC 10231) was ~ 9.5 mm. The strongest inhibitory activity of R. x damascena essential oil against Gram-negative Proteus mirabilis (ATCC 43071) was with the diameter of the inhibition zone (~ 9 mm), which was equal to the strength of rifampin (~ 9 mm). Conclusion: Therefore, this essential oil is a promising natural option rich in fatty acids, which can be a potential for the production of natural antimicrobials against infectious diseases, especially urinary tract infections. [ABSTRACT FROM AUTHOR]

This study explores the biotransformation of phenolic compounds in Rosa rugosa through Lactobacillus plantarum fermentation, enhancing their bioaccessibility and antioxidant capacity. We developed a sensitive and reproducible analytical method using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS), enabling the analysis of 17 phenolic compounds from Rosa (R) and fermented Rosa (FR). Additionally, we conducted a density functional theory (DFT) study to correlate the structure of key phenolic compounds from R and FR with their antioxidant activity. Our findings revealed that both R and FR mitigate oxidative stress in tert-butyl-hydrogen peroxide (TBHP)-induced Caco-2 and HT-29 cells by elevating the activities of crucial antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GR). Furthermore, fermented Rosa significantly upregulated Nrf2, γ-GCS, HO-1, and NOQ-1 mRNA expression in TBHP-induced cells with Quantitative and real-time PCR technology, emphasizing its protective function primarily through the Nrf2 signaling pathway. This study is the first to demonstrate the link between the enhanced antioxidant potential in fermented Rosa and the biotransformation of its phenolic compounds. It paves the way for augmenting the antioxidant capacity of plant foods through Lactobacillus plantarum fermentation, offering a novel approach to reinforce their health benefits. [ABSTRACT FROM AUTHOR]

This essay seeks to scrutinize Kierkegaard's critical philosophical theology. The intent is to demonstrate how his religious thought, especially on God's relation to the world and to the human being, can contribute to generating a cogent response to the challenges presented by our accelerating secular world. Apart from the narrative on the Dane's passionate reflections, I employ two other narratives to facilitate this inquiry into Kierkegaard. The first of these facilitating narratives comes from highlighting the work on the concept of resonance by the social theorist Hartmut Rosa. Rosa's rich analysis of our contemporary situation provides a persuasive case for the accelerating pace of our secular world, the complex dynamics of alienation that are at play within it, and the need for social transformation that creates space for increasing resonance within personal and social relationships and structures. The second facilitating narrative centers on the notion of dancing in God, which I believe holds promise for effectively communicating moving, bodily, rhythmic, passionate, and responsive thoughts and actions concerning God's engagement in our contemporary world. I hope to show that these three complementary discourses together provide a provocative religious discourse and vision that can prove helpful in addressing many of the challenges of our time. [ABSTRACT FROM AUTHOR]

Background: The primary objective of this study was to determine the accuracy and precision of component positioning of the ROSA Robotic System for total knee arthroplasty (TKA). Methods: A Preferred Reporting Items for Systematic Reviews and Meta-Analysis systematic review was conducted using 4 electronic databases (MEDLINE, EMBASE, Pubmed, and Cochrane Library) to identify all clinical and radiological studies reporting information about the use and results of the ROSA system. The criteria for inclusion were published research articles evaluating the accuracy of component positioning, learning curve, component alignment, complications, and functional outcomes in adults who underwent robotic-assisted TKA. The National Institutes of Health Quality Assessment Tool was used to evaluate the quality of all the included studies. Results: A total of 26 studies were assessed for eligibility, and 17 met the inclusion criteria. Nine studies reported on the accuracy and precision of component positioning. The ROSA platform for TKA had a cutting error of less than 0.6° for all coronal and sagittal parameters. Pooled analysis demonstrated accuracy within 0.61-1.87° and precision within 0.97-1.34° when the final intraoperative plan was compared to postoperative radiographs with fewer outliers. Four studies reported improved functional scores with ROSA-assisted TKA than conventional TKA within 1 year of surgery. There was no difference in overall complication rates when compared to conventional TKA. Conclusions: The ROSA system is both highly accurate and precise, with fewer outliers when analyzed at various time points, including postoperative standing radiographs. Future studies with robust methodology and longer follow-up are required to demonstrate whether these findings have any clinical benefits in the long term.

IntroductionPrickles are often deemed undesirable traits in many crops, including roses (Rosa sp.), and there is demand for rose cultivars with no or very few prickles. This study aims to identify new and/or validate reported quantitative trait loci (QTLs) associated with stem and leaf rachis prickle density, characterize the effects of functional haplotypes for major QTLs, and identify the sources of QTL-alleles associated with increased/decreased prickle density in roses.MethodsQTL mapping using pedigree-based analysis (PBA), and haplotype analysis were conducted on two multi-parental diploid rose populations (TX2WOB and TX2WSE).Results and discussionTwelve QTLs were identified on linkage groups (LGs) 2, 3, 4, and 6. The major QTLs for the stem prickle density were located between 42.25 and 45.66 Mbp on chromosome 3 of the Rosa chinensis genome assembly, with individual QTLs explaining 18 to 49% of phenotypic variance (PVE). The remaining mapped QTLs were minor. As for the rachis prickle density, several QTLs were detected on LG3, 4, and 6 with PVE 8 to 17%. Also, this study identified that ancestors R. wichurana ‘Basye’s Thornless’, ‘Old Blush’, and the pollen parent of M4-4 were common sources of favorable alleles (q) associated with decreased prickle density, whereas ’Little Chief’ and ‘Srche Europy’ were the source of unfavorable alleles (Q) in the TX2WOB and TX2WSE populations, respectively. The outcomes of this work complement other studies to locate factors that affect prickle density. These results can also be utilized to develop high-throughput DNA tests and apply parental selection to develop prickle-free rose cultivars.

In many plant species, petal abscission can be considered the final step of petal senescence. Cytokinins (CKs) are powerful suppressors of petal senescence; however, their role in petal abscission is ambiguous. Here, we observed that, in rose (Rosa hybrida), biologically active CK is accumulated during petal abscission and acts as an accelerator of the abscission process. Using a combination of reverse genetics, and molecular and biochemical techniques, we explored the roles of a LESION SIMULATING DISEASE1 (LSD1) family member RhLOL1 interacting with a bHLH transcription factor RhILR3 in CK-induced petal abscission. Silencing RhLOL1 delays rose petal abscission, while the overexpression of its ortholog SlLOL1 in tomato (Solanum lycopersicum) promotes pedicel abscission, indicating the conserved function of LOL1 in activating plant floral organ abscission. In addition, we identify a bHLH transcription factor, RhILR3, that interacts with RhLOL1. We show that RhILR3 binds to the promoters of the auxin signaling repressor auxin/indole-3-acetic acid (Aux/IAA) genes to inhibit their expression; however, the interaction of RhLOL1 with RhILR3 activates the expression of the Aux/IAA genes including RhIAA4-1. Silencing RhIAA4-1 delays rose petal abscission. Our results thus reveal a RhLOL1-RhILR3 regulatory module involved in CK-induced petal abscission via the regulation of the expression of the Aux/IAA genes.

Abstract Background Mechanical alignment with total knee arthroplasty (TKA) has been widely used since the implantation of the first prosthetic knee. Multiple studies have reported 80% patient satisfaction with TKA. However, the reported patients’ dissatisfaction is believed to be caused by having to convert different knee alignments to neutral alignments. It is postulated that a change in the CPAK classification of knees leads to patient dissatisfaction. Thus, we hypothesized that a change in CPAK classification with robot-assisted TKA with mechanical alignment does not significantly lead to patient dissatisfaction. Methods We retrospectively analyzed 134 patients who underwent robot-assisted mechanical alignment total knee arthroplasty (MA-TKA) using cementless implants and classified them into CPAK system pre- and post-operatively. One year after TKA surgery, we recorded binary responses to patients’ satisfaction with the outcome of surgery and analyzed if a change in CPAK classification is associated with the outcome of surgery. Results We found that 125 out of 134 patients (93.28%) were happy with the outcome of surgery. CPAK classification was changed in 116 patients (86.57%) and maintained in 18 patients (13.43%). Our results also showed that 111 (95.7%) out of 116 patients who had a change in CPAK and 14 (77.8%) out of 18 patients who maintained their CPAK post-surgery were happy with the outcome of surgery (OR = 6.3, CI 1.741–25.17, P value = 0.019). Conclusion We concluded that changing the patient’s native joint line and CPAK classification does not significantly change the outcome of surgery in terms of satisfaction. The dissatisfaction rate of 20% as published by previous researchers may be confounded by other reasons and not just due to changes in alignment and joint line.

All cavefishes, living exclusively in caves across the globe, exhibit similar phenotypic traits, including the characteristic loss of eyes. To understand whether such phenotypic convergence shares similar genomic bases, here we investigated genome-wide evolutionary signatures of cavefish phenotypes by comparing whole-genome sequences of three pairs of cavefishes and their surface fish relatives. Notably, we newly sequenced and generated a whole-genome assembly of the Chinese cavefish Triplophysa rosa. Our comparative analyses revealed several shared features of cavefish genome evolution. Cavefishes had lower mutation rates than their surface fish relatives. In contrast, the ratio of nonsynonymous to synonymous substitutions (ω) was significantly elevated in cavefishes compared to in surface fishes, consistent with the relaxation of purifying selection. In addition, cavefish genomes had an increased mutational load, including mutations that alter protein hydrophobicity profiles, which were considered harmful. Interestingly, however, we found no overlap in positively selected genes among different cavefish lineages, indicating that the phenotypic convergence in cavefishes was not caused by positive selection of the same sets of genes. Analyses of previously identified candidate genes associated with cave phenotypes supported this conclusion. Genes belonging to the lipid metabolism functional ontology were under relaxed purifying selection in all cavefish genomes, which may be associated with the nutrient-poor habitat of cavefishes. Our work reveals previously uncharacterized patterns of cavefish genome evolution and provides comparative insights into the evolution of cave-associated phenotypic traits.

(3 Z ,6 Z ,9 Z)-3,6,9-henicosatriene was identified as a major component of female sex pheromone of Bastilla arctotaenia (Lepidoptera: Erebidae), a pest of cultivated roses, by gas chromatograph-electroantennographic detector(GC-EAD) and gas chromatograph/mass spectrometry (GC/MS) analyses. The single (3 Z ,6 Z ,9 Z)-3,6,9-henicosatriene (1.0 mg/lure) successfully attracted B. arctotaenia males in the field. [ABSTRACT FROM AUTHOR]

Copyright of Flora Montibérica is the property of Flora Montiberica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Mechanical alignment with total knee arthroplasty (TKA) has been widely used since the implantation of the first prosthetic knee. Multiple studies have reported 80% patient satisfaction with TKA. However, the reported patients' dissatisfaction is believed to be caused by having to convert different knee alignments to neutral alignments. It is postulated that a change in the CPAK classification of knees leads to patient dissatisfaction. Thus, we hypothesized that a change in CPAK classification with robot-assisted TKA with mechanical alignment does not significantly lead to patient dissatisfaction. Methods: We retrospectively analyzed 134 patients who underwent robot-assisted mechanical alignment total knee arthroplasty (MA-TKA) using cementless implants and classified them into CPAK system pre- and post-operatively. One year after TKA surgery, we recorded binary responses to patients' satisfaction with the outcome of surgery and analyzed if a change in CPAK classification is associated with the outcome of surgery. Results: We found that 125 out of 134 patients (93.28%) were happy with the outcome of surgery. CPAK classification was changed in 116 patients (86.57%) and maintained in 18 patients (13.43%). Our results also showed that 111 (95.7%) out of 116 patients who had a change in CPAK and 14 (77.8%) out of 18 patients who maintained their CPAK post-surgery were happy with the outcome of surgery (OR = 6.3, CI 1.741–25.17, P value = 0.019). Conclusion: We concluded that changing the patient's native joint line and CPAK classification does not significantly change the outcome of surgery in terms of satisfaction. The dissatisfaction rate of 20% as published by previous researchers may be confounded by other reasons and not just due to changes in alignment and joint line. [ABSTRACT FROM AUTHOR]

O filme Barbie teve a maior bilheteria do ano de 2023 e, como consequência, recebeu alguns prêmios, dentre eles, o Globo de Ouro de Realização Cinematográfica. Todo o sucesso se deve apenas à qualidade do filme? O objetivo deste trabalho é analisar algumas estratégias de divulgação do filme Barbie na internet, mobilizando determinados conceitos de Walter Benjamin (1975) e de Michel Pêcheux (2014). Para isso, efetuamos uma análise documental, de cunho qualitativo, utilizando como corpus bruto a divulgação do filme Barbie realizada na internet (Google, TikTok e Twitter). Neste texto, tentaremos deslocar as reflexões e os conceitos apresentados por Walter Benjamin e Michel Pêcheux para o nosso momento atual. As reflexões realizadas a partir do corpus bruto são destacadas no decorrer do trabalho, efetuando um movimento entre a teoria e a prática durante todo o percurso, seguindo o estilo de organização de Benjamin. Ao analisar as estratégias de divulgação do filme Barbie, constatamos uma valorização referente à cor rosa e um investimento do uso das redes sociais e do humor, já que o público-alvo concerne aos jovens.

Introduction and purpose: Musculoskeletal disorders are currently among the most common occupational diseases in the healthcare system. The present study aimed to investigate the prevalence of musculoskeletal disorders among employees of Shahid Shukri Dental Hospital using ROSA and RULA methods. Methods: A cross-sectional descriptive study was conducted in Shahid Shukri Dental Hospital in 2022. The sample size was considered a census of all employees. Fifty-five computer users and 42 dentists were included in the study. Data were collected by two posture assessment methods, RULA and ROSA. The collected data was analyzed in SPSS software (version 26) and with the help of descriptive statistics and statistical t-test, Fisher's exact test, and logistic regression. Results: Based on the scores obtained from the ROSA method, the risk of musculoskeletal disorders was in the warning zone, and according to the RULA method, all the employees’ postures were at the priority levels of 2 and 3 of corrective measures. In addition, more work experience and high number of working hours per day were effective factors in the occurrence of symptoms in the neck and wrist/hand areas. Conclusion: Findings of the present study revealed that the current conditions in Shahid Shukri Dental Hospital are not suitable, and changes and improvements of conditions and workstations are required. To reduce and prevent the spread of musculoskeletal disorders, it is recommended to eliminate deficiencies through designing and implementing an educational intervention program combined with engineering interventions.

Introduction: Roses are one of the most essential ornamental flowers and are commonly used in perfumery, cosmetics, and food. They are rich in bioactive compounds, which are of interest for therapeutic effects. Objectives: The objective of this study was to understand the kinds of changes that occur between the nocturnal and diurnal metabolism of rose and to suggest hypotheses. Methods: Reversed-phase ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry or triple quadrupole mass spectrometry (TQ MS/MS) was used for nontargeted metabolomics and hormonal profiling respectively. For metabolite annotation, accurate mass spectra were compared with those in databases. Results: The hormonal profile of flowers showed an increase in jasmonate at night, while that of leaves indicated an increase in the salicylic acid pathway. Nontargeted analyses of the flower revealed a switch in the plant's defense mechanisms from glycosylated metabolites during the day to acid metabolites at night. In leaves, a significant decrease in flavonoids was observed at night in favor of acid metabolism to maintain a level of protection. Moreover, it might be possible to place back some of the annotated molecules on the shikimate pathway. Conclusion: The influence of day and night on the metabolome of rose flowers and leaves has been clearly demonstrated. The hormonal modulations occurring during the night and at day are consistent with the plant circadian cycle. A proposed management of the sesquiterpenoid and triterpenoid biosynthetic pathway may explain these changes in the flower. In leaves, the metabolic differences may reflect night-time regulation in favor of the salicylic acid pathway. [ABSTRACT FROM AUTHOR]

The movie Barbie was the highest-grossing movie in 2023, and, as a result, it received many awards, including the Golden Globe for Cinematic and Box Office Achievement. Is all the success due solely to the quality of the movie? The aim of this paper is to analyze some strategies used to promote the movie Barbie on the internet, considering the concepts discussed by Walter Benjamin (1975) and Michel Pêcheux (2014). To achieve our goal, a qualitative documentary analysis was carried out by using as raw corpus the promotion of the movie on the internet (Google, TikTok, and Twitter). In this paper, we will apply the reflections and concepts outlined by Walter Benjamin and Michel Pêcheux to our contemporary context. The analysis based on the raw corpus is highlighted throughout the paper, according to Benjamin's style of organization between theory and practice. When analyzing the strategies for promoting the Barbie movie, we found an appreciation of the color pink and an investment in the use of social networks and humor, since the target audience concerns young people. [ABSTRACT FROM AUTHOR]

Background: Robotic-assisted total knee arthroplasty (RTKA) and navigated total knee arthroplasty (NTKA) have shown improved knee alignment and reduced radiographic outliers. Recent studies have proven that conventional mechanical alignment may not be the optimal goal for every patient. The aim of this study was to compare the accuracy of the planned implant positioning of a novel image-less robotic technique with an established navigated technique (NTKA). Method: The study is a retrospective analysis of prospectively collected data that compared the implant positioning and lower-limb alignment of 86 image-less RTKA with 86 image-less NTKA. Radiographic analysis was performed to evaluate the lower-limb overall alignment, femoral and tibial components positioning in the coronal and sagittal planes. Outliers were evaluated with a cutoff of ± 3°. Results: No difference was noted between the two groups for radiographic outliers within ± 3° from neutral (p = 0.098). The mean hip–knee–ankle angle deviation from target was 1.3° in the RTKA group compared to 1.9° in the NTKA (p < 0.001). Femoral sagittal deviation (femoral component flexion) was smaller in the RTKA group (0.9° vs 1.9°; p < 0.001). Similarly, tibial coronal deviation (0.8° vs 1.5°; p < 0.001) and tibial sagittal deviation (tibial slope) were smaller in the RTKA group compared to the NTKA group (0.9° vs 1.7°; p < 0.001). Conclusions: The RTKA group reported a substantial and significant reduced error from the planned target angles for both tibial and femoral components. No difference in terms of radiographic outliers was noted between navigation and robotic assistance. [ABSTRACT FROM AUTHOR]

Copyright of Biblos: Revista da Faculdade de Letras da Universidade de Coimbra is the property of Imprensa da Universidade de Coimbra and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Agronomía Colombiana is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: Limited published data regarding the ROSA (Robotic Surgical Assistant) learning curve exist. This study evaluated the number of cases needed for an expert orthopaedic surgeon to master the ROSA system and match the operative time of robotic (raTKAs) and manual primary total knee arthroplasties (mTKAs). Methods: This retrospective comparative cohort study included two hundred patients with primary knee osteoarthritis. The study group consisted of an expert surgeon's first 100 raTKAs. The control group included 100 patients that underwent mTKAs from the same surgeon during the same period. The consecutive cases in each group were divided into ten subgroups, each of 10 cases. The groups were comparable concerning age, sex, BMI and Kellgren–Lawrence classification. We compared each subgroup's operative time and complications in mTKA and raTKA groups. We performed a cumsum analysis to construct the ROSA learning curve. Results: The first non-significant difference between the mTKAs and raTKAs operative times was observed in the subgroup of 62 to 71 cases. Till then, the operative time has been significantly lower for the mTKA than the raTKA group. The following groups of tens analysis (8th, 9th and 10th) showed no operative time difference between groups. The learning curve analysis demonstrated that the surgeon switched to the mastering phase from case 73 onwards. The two groups had no complication rate differences. Conclusion: Our study demonstrated that about 70 cases are necessary for a senior surgeon to balance operative time between mTKAs and raTKAs using the ROSA system. [ABSTRACT FROM AUTHOR]

We investigated the potential of markers associated with floral traits for parental selection in a cut rose breeding program. We analysed six Kompetitive Allele Specific PCR (KASP) markers for three important floral traits, petal length, petal number and scent, derived from experiments in a garden rose population. The six markers were applied to genotype a collection of 384 parental genotypes used for commercial cut rose breeding. We phenotyped a selection of progeny derived from pairs of parents having either high or low dosages of (contrasting) marker alleles associated with these traits. Significant differences were found between the contrasting progeny groups for each of the traits, although parents with the optimal allele dosage combinations could not always be used for the crosses. This not only supports the robustness of these marker‒trait associations but also demonstrates their potential for commercial rose breeding. It also demonstrates the use of marker information generated in garden rose populations for cut rose breeding. [ABSTRACT FROM AUTHOR]

Total knee arthroplasty (TKA) is a successful and highly effective procedure in most patients with tricompartmental knee arthritis. Despite the innovations in surgical techniques due to planning software and technological innovations, patients' dissatisfaction after TKA is still high, at up to 20%. Robotic-assisted surgery (RAS) could be considered as a future option for improving outcomes due to its higher accuracy, precision, and reliability. Robotic systems can be classified as fully active, semi-active, or passive depending on the surgeon's involvement during the procedure, and as imageless or image-based according to the necessity of radiological exams for the pre-operative planning. Three of the most well-known robotic systems for knee surgery are MAKO® (Stryker Ltd., Kalamazoo, MI, USA), NAVIO® (Smith & Nephew, Andover, TX, USA), and ROSA® (Zimmer Inc., Warsaw, IN, USA). These systems show differences in terms of surgeon involvement, the use of CT scans or X-rays for pre-operative planning, the possibility to perform both unicompartmental knee arthroplasty (UKA) and TKA (or even total hip arthroplasty THA), and in the different kinds of knee prosthesis that can be implanted. This article aims to describe the features of the most used robotic systems for knee arthroplasty, to examine their outcomes and analyze their cost-effectiveness, and to evaluate future perspectives. [ABSTRACT FROM AUTHOR]

Reactive oxygen species (ROS) are unstable reactive molecules that are toxic to cells. Regulation of ROS homeostasis is crucial to protect cells from dysfunction, senescence, and death. In plant leaves, ROS are mainly generated from chloroplasts and are tightly temporally restricted by the circadian clock. However, little is known about how ROS homeostasis is regulated in nonphotosynthetic organs, such as petals. Here, we showed that hydrogen peroxide (H2O2) levels exhibit typical circadian rhythmicity in rose (Rosa hybrida) petals, consistent with the measured respiratory rate. RNA-seq and functional screening identified a B-box gene, RhBBX28, whose expression was associated with H2O2 rhythms. Silencing RhBBX28 accelerated flower senescence and promoted H2O2 accumulation at night in petals, while overexpression of RhBBX28 had the opposite effects. RhBBX28 influenced the expression of various genes related to respiratory metabolism, including the TCA cycle and glycolysis, and directly repressed the expression of SUCCINATE DEHYDROGENASE 1, which plays a central role in mitochondrial ROS (mtROS) homeostasis. We also found that PHYTOCHROME-INTERACTING FACTOR8 (RhPIF8) could activate RhBBX28 expression to control H2O2 levels in petals and thus flower senescence. Our results indicate that the circadian-controlled RhPIF8-RhBBX28 module is a critical player that controls flower senescence by governing mtROS homeostasis in rose.

The phytohormone auxin plays a pivotal role in floral meristem initiation and gynoecium development, but whether and how auxin controls floral organ identity remain largely unknown. Here, we found that auxin levels influence organ specification, and changes in auxin levels influence homeotic transformation between petals and stamens in rose (Rosa hybrida). The PIN-FORMED-LIKES (PILS) gene RhPILS1 governs auxin levels in floral buds during floral organogenesis. RhAUXIN RESPONSE FACTOR 18 (RhARF18), whose expression decreases with increasing auxin content, encodes a transcriptional repressor of the C-class gene RhAGAMOUS (RhAG), and controls stamen-petal organ specification in an auxin-dependent manner. Moreover, RhARF18 physically interacts with the histone deacetylase (HDA) RhHDA6. Silencing of RhHDA6 increases H3K9/K14 acetylation levels at the site adjacent to the RhARF18-binding site in the RhAG promoter and reduces petal number, indicating that RhARF18 might recruit RhHDA6 to the RhAG promoter to reinforce the repression of RhAG transcription. We propose a model for how auxin homeostasis controls floral organ identity via regulating transcription of RhAG.

Introduction: Office workers, regardless of educational principles, may be exposed to musculoskeletal injuries due to the nature of their work. Therefore, the present study was conducted to investigate the effect of ergonomics training in reducing musculoskeletal disorders among the administrative staff of an Ilam University of Medical Sciences campus. Materials and Methods: In this descriptive-analytical study, 178 administrative staff of Ilam University of Medical Sciences Campus 1 was examined. Musculoskeletal disorders were evaluated using the Nordic questionnaire. Ergonomic risk factors of the workplace were assessed by Rapid Administrative Strain Assessment (ROSA) before and after the training. Data analysis was done through SPSS version 26 statistical software. Results: According to the findings obtained from the Nordic questionnaire, it was found that the most disorders in the past year were in the back (56.2%) and neck (55.6%) areas and the ROSA evaluation results showed that 24.4% of the subjects the study was at the ergonomic level with high risk. After the implementation of training programs, the results showed that the prevalence of disorders in 9 areas decreased. Also, demographic variables did not have a significant effect on the prevalence of musculoskeletal disorders. (Significance level 0/05 is assumed) Conclusion: The results of this study showed that ergonomic training can be an effective method to reduce musculoskeletal discomfort in office work environments.

Introduction: The most common occupational diseases are musculoskeletal disorders that happen with improper body posture while performing job duties. The present study aimed to determine the prevalence of musculoskeletal disorders and evaluate the physical condition of hospital employees using REBA and ROSA methods. Materials and methods: This descriptive-analytical study was conducted on 125 employees of Dezful teaching hospital, selected by stratified random sampling method. Musculoskeletal disorders were assessed using the Nordic questionnaire, and Rapid Entire Body Assessment (REBA)، and Rapid Office Strain Assessment (ROSA) methods were used to assess physical condition. The normality of the data was checked using Kolmogorov-Smirnov test. Statistical analysis was done with SPSS version 23 software. Results: The average age and work experience were 36.49±8.26 and 7.33±11.8 years, respectively. The highest prevalence of musculoskeletal disorders was related to the neck (47.7%) and wrist (40.4%), and the lowest was the elbow (7.8%). According to the REBA score, 18.3% of posture workers needed immediate corrections, and 37.8% needed corrections as soon as possible. According to the ROSA score, in 75.8% of the tasks, the work environment should be re-evaluated. There was a significant relationship (P-value