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Background: Zinc nanoparticles (NPs) are characterized by high bioavailability, small size, and high absorbability. The purpose of this experiment was to determine the effect of Zn-NP feed supplementation on ruminal fermentation, microbiota, and histopathology in lambs. In vitro (24 h), short-term (STE, 28 d), and long-term (LTE, 70 d) experiments were performed. The lambs in STE were fed a basal diet (BD) composed of 350 g/d ground barley and 700 g/d meadow hay (Control), BD enriched with ZnO-NPs (80 mg Zn/kg of diet, ZnO-NPs), and BD enriched with Zn phosphate-based NPs (80 mg Zn/kg of diet, ZnP-NP). The in vitro gas production technique was used in incubated rumen fluid from STE. The lambs in LTE were fed BD (Control), BD enriched with ZnO-NPs (40 mg Zn/kg of diet, ZnO-NP40), BD enriched with ZnO-NPs (80 mg Zn/kg of diet, ZnO-NP80) and BD enriched with ZnO (80 mg Zn/kg of diet, ZnO-80). Results: After 24 h of incubation, dry matter digestibility was higher for ZnO-NP and ZnP-NP substrates than the control in an in vitro experiment (P < 0.001). The total bacterial population in the STE was lower (P < 0.001) in the ZnP-NP group than in the control and ZnO-NP groups, but the protozoan populations were not significantly different. The ammonia-N concentration in LTE was lowest in the ZnO-NP80 group (P = 0.002), but the activities of carboxymethyl cellulase (P < 0.001) and xylanase (P = 0.002) were higher in the ZnO-NP40, ZnO-NP80, and ZnO-80 groups than in the control group. Morphological observation after STE and LTE revealed histological changes (e.g. inflammation of the epithelium or edema of the connective tissue) in the rumen of lambs. Conclusion: Zn-NP supplementation up to 70 d improved feed-use efficiency and influenced ammonia-N concentration and activities of hydrolases in the rumen. The active ruminal fermentation affected the health of the ruminal papillae and epithelium in the lambs, regardless of the application's form, dose, or duration. However, by affecting rumen microbial fermentation, Zn-NPs could alter fermentation patterns, thereby increasing the capacity of host rumen epithelial cells to transport short-chain fatty acids. [ABSTRACT FROM AUTHOR]

Background: Shortening the fattening period can reduce production costs and inedible fat, but may simultaneously negatively affect productivity and profitability. We hypothesized that increasing the crude protein and total digestible nutrients (TDN) in the formula feed could address the issues associated with shortened fattening periods without adversely affecting rumen fermentation. This study investigated the effect of a simultaneous increase of crude protein and TDN contents compared to conventional formula feed on growth performance, blood metabolites, carcass characteristics and meat composition of late fattening Hanwoo steers. Methods: Thirty Hanwoo steers were randomly assigned to one of two dietary groups: control group (16.4% crude protein and 83.6% TDN based on dry matter) and treatment group (17.4% crude protein and 84.6% TDN based on dry matter). Result: The rumen parameters were similar between the control and treatment groups. Likewise, the effect of additional crude protein and TDN contents in the formula feed showed no significant impact on the growth performance or blood metabolites. The carcass weight and yield index were slightly higher in the treatment group compared to the control, yet these differences were not statistically significant. Furthermore, the dietary treatment did not affect marbling score, meat color, fat color, texture, maturity, pH, surface colors, drip loss, cooking loss, sensory characteristics, or fatty acid composition in the longissimus muscle. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. This study aimed to assess the effect of bulk tank milk (BTM), waste milk (WM), and pasteurized waste milk (PWM) on nutrient digestibility, ruminal and cecal fermentation, gastrointestinal tract (GIT) development, and antimicrobial resistance of fecal Escherichia coli from dairy calves at 2 periods (30 and 60 d of age). Calves were grouped according to BW, serum protein levels, and breed composition. Three treatments were included: BTM (n = 21), WM from cows under antibiotic treatment (n = 21), and PWM (WM submitted to HTST pasteurization; n = 21). A total of 63 calves were used, of which 18 animals (n = 6 per treatment) were evaluated in the period of 4 to 30 d, and 45 (n = 15 per treatment) from 4 to 60 d. During the experimental period, a daily intake of 6 L of milk was divided into 2 equal meals, with ad libitum access to water and starter. Milk and feed intakes were recorded daily. Apparent total-tract digestibility and nitrogen balance were conducted from 25 to 29 d of age (n = 6) and from 53 to 57 d of age (n = 15). Animals were slaughtered at 30 ± 1 and 60 ± 1 d of age for the assessment of ruminal and cecal fermentation and GIT development. Antimicrobial susceptibility testing was conducted at 1, 30, and 60 d of age (n = 15/treatment). Statistical analysis used a linear mixed-effects model for continuous outcomes and generalized linear models for single measurements (R software). Treatments WM and PWM had lower rumen pH, higher ruminal acetate concentration, larger reticulorumen and liver, and a higher prevalence of fecal-resistant E. coli compared with BTM at both 30 and 60 d. Up to 60 d, both BTM and WM treatments exhibited higher digestibility of ether extract and gross energy compared with the PWM, whereas WM and PWM treatments showed increased nitrogen intake and retention compared with the BTM treatment. These findings suggest that pasteurization of WM negatively affects nutrient digestibility and calf performance, while also affecting rumen development. Additionally, the use of milk containing antibiotic residue leads to the selection of resistant E. coli in the GIT over time. [ABSTRACT FROM AUTHOR]

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Simple Summary: Bovine papillomavirus (BPV) infection of the gastrointestinal tract (GIT) can induce the development of masses with high growth, resulting in breathing and eating obstructions leading to animal suffering and death. Beyond this, BPV is related to economic losses worldwide by depressing meat and milk production as well as cattle by-products. Using PCR followed by Sanger sequencing, we were able to identify the high-risk Delta BPVs and the BPV44 on rumen cattle samples collected in slaughterhouses. These results can contribute to future epidemiological studies and vaccine studies regarding BPV infections. The Bos Taurus Papillomavirus, commonly known as bovine papillomavirus (BPV), can cause lesions in the mucosa of the gastrointestinal tract (GIT) in cattle and induce the formation of papillomas in organs such as the pharynx, esophagus, rumen and reticulum. GIT papillomas can lead to feeding and breathing distress. Moreover, the sample collection is challenging, which reduces the BPV diagnosis in these organs. BPV can cause exophytic nodular, cauliflower-like, flat, filiform or atypical-shape papillomas at the epidermis. Histologically, the papillomas demonstrate orthokeratotic/parakeratotic hyperkeratosis and koilocytosis and, currently, BPV comprises 45 described types. The aim of this study was to carry out the genetic characterization of BPV present in rumen neoplastic lesions of cattle raised extensively in the Western Amazon region, Brazil. A total of 100 papillomatous ruminal samples were collected from animals slaughtered in Ji-Paraná and Urupá municipalities from the Rondônia state, Brazil. The samples were submitted to PCR using the primer pair FAP59/FAP64 and sequenced by the Sanger method. Histopathological analysis was performed on 24 samples, which had enough material for this purpose. As a result, samples were histologically classified as fibropapilloma and squamous papilloma. Among the samples analyzed, it was possible to identify the BPVs 2, 13 (Delta PVs) and 44, with one sample classified as a putative new subtype of BPV44. The present study could identify BPV13 and 44 types in cattle rumen tissues from the Brazilian Amazon region for the first time. [ABSTRACT FROM AUTHOR]

The Regional Veterinary Laboratories in Ireland conducted necropsy examinations and diagnostic testing on various carcasses, fetuses, and samples in May 2024. The report provides feedback to veterinary practitioners on the patterns of disease syndromes during this time, highlighting common and unusual cases in cattle and sheep. The findings emphasize the importance of parasite control, husbandry practices, and disease prevention measures in livestock farming. The document also discusses specific conditions and diseases, such as tick-borne fever with secondary bacterial infections, pneumonia, intestinal torsion/volvulus, mastitis, and abscessation. [Extracted from the article]

The layout of methane sensors in the working face cannot meet the needs for monitoring methane concentrations within confined spaces, and it is challenging to determine the precise locations for manual inspections. Therefore, the working face is firstly divided into different areas and grids. Then combined with the characteristics of methane emissions and the measured data on site, the boundary conditions of simulation experiments are set up and the research is carried out on the diffusion law of methane in the confined space of the working face under different conditions. The experimental results show that methane emission intensity from coal walls affects its distribution. As emission intensity rises, methane nearer the coal wall decreases, while methane further away increases. Among coal mining points, point 2 shows the widest methane diffusion range. Rising wind speeds decrease methane diffusion from the coal wall, increasing vertical diffusion distance. Methane from the coal wall shifts to the air inlet, while methane from the mining point diffuses increasingly to the downwind side. The location of the maximum methane concentration generated from falling coal and its transportation process is only related to the location of the coal mining point. The key areas for methane monitoring in confined spaces of the working face should be the overlapping locations of the vertical‐3 and vertical‐4 areas and the horizontal‐1 and horizontal‐3 areas. The key areas for manual inspection should be the overlapping locations of the vertical‐2 and vertical‐3 areas and the horizontal‐1 area. [ABSTRACT FROM AUTHOR]

Maintaining cleaner and more sustainable ecosystems by mitigating greenhouse gas (GHG) emissions from livestock through dietary manipulation is in demand. This study was aimed to assess the effect of Moringa oleifera seeds and probiotics (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) as feed supplements on GHG production and fermentation profile from steers and sheep. The treatments included diets containing 0, 6, 12, and 18% of M. oleifera seeds meal and a mixture of probiotic bacteria (0.2 ml/g of diet). Total biogas production, CH4, CO, and H2S emission from animals (up to 48 h), rumen fermentation profile, and CH4 conversion efficiency were recorded using standard protocols. Results showed interaction among M. oleifera seeds and probiotics on asymptotic biogas production and total biogas production up to 48 h (P < 0.05). The rate of CH4 emission in steers was reduced from 0.1694 to 0.0447 ml/h using 6 and 18% of M. oleifera seeds (P < 0.05). Asymptotic CO and the rate of CO production were increased (P < 0.05) by supplementing different doses of M. oleifera seeds and probiotics. Adding 12% of M. oleifera seeds and probiotics reduced H2S production from 0.0675 to 0.0112 ml H2S/g DM (at 48 h of fermentation) in steers. In sheep, the additives mitigated H2S production from 0.0364 to 0.0029 ml H2S/g DM (at 48 h of fermentation), however there were not interaction (P = 0.7744). In addition, M. oleifera seeds and probiotics reduced the pH level and dry matter degradability (DMD) in steers and sheep (P < 0.0001) showing a positive impact on CH4:ME and CH4:OM (in steers) and CH4:SCFA (in sheep), while the interaction was not significant (P > 0.05) for CH4:SCFA (in steers) and CH4:ME and CH4:OM (in sheep). In conclusion, the interaction of M. oleifera seeds and probiotics in the feeding diet reduced GHG emissions and affected the fermentation profile of steers and sheep. [ABSTRACT FROM AUTHOR]

This study aims to compare rumen microbiome and metabolites between second lactation dairy cows in the 75th percentile (n = 12; 57.2 ± 5.08 kg/d) of production according to genomic predicted transmitting ability for milk (GPTAM) and their counterparts in the 25th percentile (n = 12; 47.2 ± 8.61 kg/d). It was hypothesized that the metagenome and metabolome would differ between production levels. Cows were matched by days in milk (DIM), sire, occurrence of disease, and days open in previous lactation. For an additional comparison, the cows were also divided by phenotype into high (n = 6; 61.3 ± 2.8 kg/d), medium (n = 10; 55 ± 1.2 kg/d), and low (n = 8; 41.9 ± 5.6 kg/d) based on their milk production. Samples were collected 65 ± 14 DIM. Rumen content was collected using an oro-gastric tube and serum samples were collected from the coccygeal vessels. High-resolution liquid chromatography-mass spectrometry (LC-MS) was used for rumen and serum metabolite profiling. Shotgun metagenomics was used for rumen microbiome profiling. Microbiome sample richness and diversity were used to determine alpha and Bray-Curtis dissimilarity index was used to estimate beta diversity. Differences in metabolites were determined using t-tests or ANOVA. Pearson correlations were used to consider associations between serum metabolites and milk production. There was no evidence of a difference in rumen metabolites or microbial communities by GPTAM or phenotype. Cows in the phenotypic low group had greater serum acetate to propionate ratio and acetate proportion compared to the cows in the phenotypic medium group. Likewise, serum propionate proportion was greater in the medium compared to the low phenotypic group. Serum acetate, butyrate, and propionate concentrations had a weak positive correlation with milk production. When investigating associations between rumen environment and milk production, future studies must consider the impact of the ruminal epithelium absorption and post-absorption processes in relation to milk production. [ABSTRACT FROM AUTHOR]

Simple Summary: Ginger and its straw contain not only gingerol and other active ingredients but also proteins, sugars, vitamins, and other nutrients, and are potential feed resources. At present, research on ginger straw feed has mainly focused on pig and poultry breeding, with that related to ruminant animal research being relatively rare. The enzyme silage treatment of ginger straw cannot only effectively be used to retain the nutrients in ginger straw, improve its palatability, and prolong the storage time of the ginger straw, but it can also effectively increase animal digestion. The Laiwu black goat is an excellent local germplasm resource. Here, the effects of enzymatic silage ginger straw silage on growth performance, nutrient digestibility, slaughter performance, muscle quality, serum biochemical indices, intestinal microflora, and antioxidant activity in Laiwu black goats were studied. The aim was to determine the effect of enzymatic silage ginger straw on Laiwu black goat feeding. Laiwu black goats comprise an excellent local germplasm resource; however, a shortage of feed resources has led to the application of unconventional feed. Ginger straw feed has good physiological effects, but research on this feed source for ruminant animals is lacking. The aim of this study was to determine the effects of enzymatic silage ginger straw on Laiwu black goat performance. The experiment used an independent sample t-test analysis method; 24 healthy Laiwu black goats with a body weight of 20.05 ± 1.15 kg and age of 5.67 ± 0.25 months were randomly divided into two groups with three replicates (bars) per group and four goats per replicate. The experimental diet was composed of mixed concentrate, silage, and garlic peel at a 2:7:1 ratio. The silage used in the two groups was whole corn silage (CON group) and 60% whole corn silage plus 40% enzymatic silage ginger straw (SG group), and the other components were identical. Daily feed intake/daily gain (F/G) was significantly higher in the SG group than in the CON group (p < 0.05), but there were no significant differences in dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility between the groups. The shear force, cooking loss, centrifugal loss, and pressure loss of the longissimus dorsi muscle group were significantly lower in the SG than in the CON group (p < 0.05). Compared with those in the CON group, the serum and liver total antioxidant capacity was significantly increased in the SG group, and in the liver, the O2·−, malondialdehyde, and OH· contents were significantly decreased. Collectively, the rumen fluid microbial diversity was changed in the SG group. It was concluded that enzymatic silage ginger straw usage instead of 40% whole silage corn as feed for Laiwu black goats can significantly improve the muscle quality, antioxidant capacity, and intestinal flora, with no adverse effects on production performance. In conclusion, our study provides a basis for ginger straw processing and storage and its rational application in the Laiwu black goat diet. [ABSTRACT FROM AUTHOR]

Although early solid diet supplementation is a common practice to improve the growth and development in goat kids, its biological mechanism how solid diet induces rumen microbiota and epithelial development is still unknow. In this study, rumen fermentation parameters, 16S rRNA sequencing for rumen content and epithelial microbiota, transcriptomics and proteomics of epithelium were determined to classify the effects of solid diet supplementation. Here, we classified the changes of goat phenotypes (i.e., growth performance, rumen fermentation and development) and linked them to the changes of rumen microbiota, transcriptome and expressed proteins. The mechanism of solid diet improving rumen development was elucidated preliminarily. Moreover, different roles between the rumen content and epithelial microbiota were identified. Thess datasets expands our understanding of the association between the early diet intervention and rumen development, providing the useful information how nutrient strategy affects rumen function and subsequently improves the host growth. The generated data provides insights in the importance of rumen niche microbiota and microbe-host interactions, which benefits future studies. [ABSTRACT FROM AUTHOR]

Cattle lameness remains a significant concern, causing economic losses and compromising animal welfare. Claw horn lesions have been identified as a major cause of lameness in dairy cows, but their correlation with high-energy diets and ruminal acidosis remains unclear. Hence, the primary objective of this study was to assess the effects of a high-starch diet and a conventional diet on the rumen environment, acute-phase proteins, and metabolic alterations, with a particular focus on insulin resistance and the consequent implications for the histology of the hooves in Holstein steers. A total of 16 animals were divided into the high-starch (HS; 37% starch) and conventional (CON; 16.8% starch) groups. Glucose tolerance tests (GTT), blood analyses, rumen fluid analyses, and histological evaluations of the hoof tissue were conducted over a 102-d experimental period. The HS group showed a lower ruminal pH than the CON group, and with values indicating SARA. The plasma glucose and IGF-1 concentrations were higher in the HS group, suggesting an anabolic state. Both groups exhibited an increase in the insulin area under the curve (AUC) after the GTT on d 102. Histological analysis of the hooves showed a reduction in the length and width of the epidermal lamella in both groups. We found a significant negative correlation between the insulin AUC and the length and width of the epidermal lamella. Because both groups were similarly affected, the hypothesis that histological alterations were caused by the experimental diets still needs confirmation. Additionally, the development of SARA was not essential for the observed histological changes in the hoof. Further studies are warranted to thoroughly investigate the role of insulin and IGF-1 imbalances in claw health. [ABSTRACT FROM AUTHOR]

The Regional Veterinary Laboratories Report for April 2024 provides information on common and unusual cases investigated by the Department of Agriculture, Food and the Marine's veterinary laboratories. The report focuses on diseases in cattle, such as pneumonia and enteritis, and discusses various other conditions found in cattle, sheep, and an emu. The article provides information on symptoms, diagnoses, and potential causes of these conditions, emphasizing the importance of timely diagnosis and treatment. Farmers are advised to monitor their animals closely and take appropriate measures for prevention and treatment. [Extracted from the article]

Heterocyclic amines (HAs) are a group of mutagenic and carcinogenic compounds produced from the processing of high‐protein foods, which include 2‐amino‐3‐methylimidazo[4, 5‐f]quinoline (IQ) showing the strongest carcinogenic effect. Camels are able to digest HAs in foods, which provide rich microbial resources for the study. Thus, camel rumen and intestinal microbiota were used to degrade IQ, and the dominant microorganisms and their degradation characteristics were investigated. After three generations of culture with IQ as the sole carbon source, the highest abundance in rumen and intestinal microbes was found in the Proteobacteria phylum. The strains of third generation of the rumen contents were mainly attributed to the genera Brevundimonas and Pseudomonas, and the dominant genera in intestine were Ochrobactrum, Bacillus, and Pseudomonas. Microorganisms were further isolated and purified from the third generation cultures. These 27 strains from the rumen (L1–L27) and 23 strains from the intestine (C1–C23) were obtained. Among them, four strains with the most effective degrading abilities were as follows: L6 (28.55% of IQ degrading rate) and C1 (25.19%) belonged to the genus Ochrobactrum, L15 (23.41%) belonged to the genus Pseudomonas, and C16 (20.89%) were of the genus Bacillus. This study suggested the application of abundant microbial resources from camels' digestive tract to biodegrade foodborne toxins. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of weaning age and pace on blood metabolites, cortisol concentration, and mRNA abundance of inflammation-related genes in Holstein dairy calves. A total of 70 1-d-old calves (38.8 ± 4.4 kg BW ± SD), blocked by sex and birth BW, were randomly assigned to a 2 × 2 factorial arrangement of treatments. The first factor was weaning age, which was either early (6 wk) or late (8 wk). The second factor was weaning pace, which was either abrupt (4 steps down over 3 d; the initial milk replacer was 7.6 L, which was reduced by 1.9 L in each step-down) or gradual (7 steps down over 14 d; the initial milk replacer was 7.6 L, which was reduced by 1.09 L in each step-down), generating early-abrupt (EA), early-gradual (EG), late-abrupt (LA), and late-gradual (LG) treatments. All treatments had 10 female and 8 male calves, except EA that had 1 fewer male calf. Milk replacer (24% CP, 17% fat) was bottle fed, up to 1,200 g/d, twice daily (0600 h and 1800 h). The EA and EG treatment calves received 46.2 kg of milk replacer, and the LA and LG treatment calves received 63 kg of milk replacer. The study had 2 cohorts (2020, n = 40; 2021, n = 31), and each cohort included all treatments. Blood was collected from the jugular vein at 0900 h at 3 and 7 d of age, and a day before starting and a day after weaning completion. Male calves were humanely killed a day after weaning. Rumen, jejunum, large intestine, liver, omental adipose and perirenal adipose tissues were sampled to determine the mRNA abundance of inflammation-related genes. Weaning pace, age, pace × age, birth BW, and sex were included as fixed and cohort was included as random effects in the model. Blood metabolites and cortisol were analyzed as repeated measures, and sampling day, pace × sampling day, and age × sampling day were also included as additional fixed effects. Significance was noted at P ≤ 0.05 and tendencies when 0.05 < P ≤ 0.10. The EA calves showed a tendency to have the greatest nonesterified fatty acid (NEFA) concentration compared with all other treatments. We observed a pace × day effect on serum NEFA and BHB; calves weaned at an abrupt pace had an increased level of NEFA after weaning compared with those weaned gradually. Calves weaned at the gradual pace showed the greatest serum BHB after weaning. Most mRNA abundances for inflammation-related genes affected by treatments showed a similar pattern. They were downregulated by the abrupt (liver IL-1β) and early weaning (jejunum TNF-α and ICAM), and in some cases, the interaction intensified the effect, demonstrating a weakened immune response in calves experiencing more stressful conditions (EA: IL-6 in the liver and NF-κB in the perirenal adipose tissue). Overall, the downregulation of the mRNA abundance of inflammation-related genes in EA calves may be attributed to the suppression of the immune system and an immature immune response. Furthermore, the greater NEFA in EA calves could be attributed to a reduced starter intake, less developed rumen, or shorter time during the weaning transition. [ABSTRACT FROM AUTHOR]

The Regional Veterinary Laboratories (RVLs) conducted necropsy examinations on 683 carcasses and 338 fetuses in March 2024, as well as testing 1,933 diagnostic samples to assist private veterinary practitioners. The report provides feedback on disease patterns, common and unusual cases, and available laboratory diagnostic tools. The most common diagnoses in cattle were enteritis and pneumonia, with rotavirus and Cryptosporidium being the most commonly detected pathogens in cases of calf diarrhea. The article also discusses various cases of diseases and conditions found in calves and sheep in Ireland, highlighting the importance of proper nutrition and management practices in preventing these conditions. [Extracted from the article]

The gastrointestinal tract (GIT) is stationed by a dynamic and complex microbial community with functions in digestion, metabolism, immunomodulation, and reproduction. However, there is relatively little research on the composition and function of microorganisms in different GIT segments in dairy goats. Herein, 80 chyme samples were taken from ten GIT sites of eight Xinong Saanen dairy goats and then analyzed and identified the microbial composition via 16S rRNA V1-V9 amplicon sequencing. A total of 6669 different operational taxonomic units (OTUs) were clustered, and 187 OTUs were shared by ten GIT segments. We observed 264 species belonging to 23 different phyla scattered across ten GITs, with Firmicutes (52.42%) and Bacteroidetes (22.88%) predominating. The results revealed obvious location differences in the composition, diversity, and function of the GIT microbiota. In LEfSe analysis, unidentified_Lachnospiraceae and unidentified_Succinniclassicum were significantly enriched in the four chambers of stomach, with functions in carbohydrate fermentation to compose short-chain fatty acids. Aeriscardovia, Candidatus_Saccharimonas, and Romboutsia were significantly higher in the foregut, playing an important role in synthesizing enzymes, amino acids, and vitamins and immunomodulation. Akkermansia, Bacteroides, and Alistipes were significantly abundant in the hindgut to degrade polysaccharides and oligosaccharides, etc. From rumen to rectum, α-diversity decreased first and then increased, while β-diversity showed the opposite trend. Metabolism was the major function of the GIT microbiome predicted by PICRUSt2, but with variation in target substrates along the regions. In summary, GIT segments play a decisive role in the composition and functions of microorganisms. Key points: • The jejunum and ileum were harsh for microorganisms to colonize due to the presence of bile acids, enzymes, faster chyme circulation, etc., exhibiting the lowest α-diversity and the highest β-diversity. • Variability in microbial profiles between the three foregut segments was greater than four chambers of stomach and hindgut, with a higher abundance of Firmicutes dominating than others. • Dairy goats dominated a higher abundance of Kiritimatiellaeota than cows, which was reported to be associated with fatty acid synthesis. [ABSTRACT FROM AUTHOR]

Simple Summary: Environmental factors like climate, diet, and farming methods significantly affect microbial activity and composition. Understanding these adjustments is crucial for improving ruminant nutrition and production. This review summarizes research on rumen microbiota and molecular methods for microbial identification in ruminant rumen fluid. Analyzing rumen microbiology in various ruminants is challenging due to environmental and nutritional influences on microbial interactions. Key genera include Entodinium spp. and Aspergillus spp. for protozoa and fungi, respectively, with Fibrobacter spp. prevalent among bacteria. Major techniques involve DNA extraction, amplification, and sequencing. In conclusion, this review highlights the knowledge in the literature about rumen microorganisms and the associated molecular approaches. Variations in environments, including climate, diet, and agricultural practices, significantly impact the composition and microbial activity. A profound understanding of these adaptations allows for the improvement of nutrition and ruminant production. Therefore, this review aims to compile data from the literature on the rumen microbiota and molecular techniques for identifying the different types of microorganisms from the rumen fluid of ruminants. Analyzing the literature on rumen microbiology in different ruminants is complex due to microbial interactions, influenced by the environment and nutrition of these animals. In addition, it is worth noting that the genera of protozoa and fungi most evident in the studies used in this review on the microbiology of rumen fluid were Entodinium spp. and Aspergillus spp., respectively, and Fibrobacter spp. for bacteria. About the techniques used, it can be seen that DNA extraction, amplification, and sequencing were the most cited in the studies evaluated. Therefore, this review describes what is present in the literature and provides an overview of the main microbial agents in the rumen and the molecular techniques used. [ABSTRACT FROM AUTHOR]

Clinical illness (CI) scoring using visual observation is the most widely applied method of detecting respiratory disease in cattle but has limited effectiveness in practice. In contrast, body-mounted sensor technology effectively facilitates disease detection. To evaluate whether a combination of movement behavior and CI scoring is effective for disease detection, cattle were vaccinated to induce a temporary inflammatory immune response. Cattle were evaluated before and after vaccination to identify the CI variables that are most indicative of sick cattle. Respiratory rate (H2 = 43.08, P < 0.0001), nasal discharge (H2 = 8.35, P = 0.015), and ocular discharge (H2 = 16.38, P = 0.0003) increased after vaccination, and rumen fill decreased (H2 = 20.10, P < 0.0001). Locomotor activity was measured via leg-mounted sensors for the four days preceding and seven days following vaccination. A statistical model that included temperature, steps, lying time, respiratory rate, rumen fill, head position, and excess saliva was developed to distinguish between scores from before and after vaccination with a sensitivity of 0.898 and specificity of 0.915. Several clinical illness signs were difficult to measure in practice. Binoculars were required for scoring respiratory rate and eye-related metrics, and cattle had to be fitted with colored collars for individual identification. Scoring each animal took up to three minutes in a small research pen; therefore, technologies that can automate both behavior monitoring and identification of clinical illness signs are key to improving capacity for BRD detection and treatment. [ABSTRACT FROM AUTHOR]

Simple Summary: The yak is the dominant livestock on the Tibetan plateau, providing milk, meat, fur, and income for the local residents; however growth-retarded yaks are of a high proportion and reduce the yak farming profitability. Our study found that the obstruction of energy metabolism and ATP synthesis in ruminal epithelium contributed to the growth retardation of yaks. It provided a fundamental understanding of how the energy metabolism of ruminal epithelium affected the growth retardation of yaks and provided new ideas for improving rumen health and the growth performance of growth-retarded yaks through nutritive regulation methods. Growth-retarded yaks are of a high proportion on the Tibetan plateau and reduce the economic income of farmers. Our previous studies discovered a maldevelopment in the ruminal epithelium of growth-retarded yaks, but the molecular mechanisms are still unclear. This study aimed to reveal how the proteomic profile in the ruminal epithelium contributed to the growth retardation of yaks. The proteome of the ruminal epithelium was detected using a high-resolution mass spectrometer. There were 52 proteins significantly differently expressed between the ruminal epithelium of growth-retarded yaks and growth-normal yaks, with 32 downregulated and 20 upregulated in growth-retarded yaks. Functional analysis showed the differently expressed proteins involved in the synthesis and degradation of ketone bodies (p = 0.012), propanoate metabolism (p = 0.018), pyruvate metabolism (p = 0.020), and mineral absorption (p = 0.024). The protein expressions of SLC26A3 and FTH1, enriched in the mineral absorption, were significantly downregulated in growth-retarded yaks. The key enzymes ACAT2 and HMGCS2 enriched in ketone bodies synthesis and key enzyme PCCA enriched in propanoate metabolism had lower protein expressions in the ruminal epithelium of growth-retarded yaks. The ATP concentration and relative mitochondrial DNA copy number in the ruminal epithelium of growth-normal yaks were dramatically higher than those of growth-retarded yaks (p < 0.05). The activities of citrate synthase (CS), the α-ketoglutarate dehydrogenase complex (α-KGDHC), isocitrate dehydrogenase (ICD) in the tricarboxylic acid cycle (TCA), and the mitochondrial respiratory chain complex (MRCC) were significantly decreased in ruminal epithelium of growth-retarded yaks compared to growth-normal yaks (p < 0.05). The mRNA expressions of COQ9, COX4, and LDHA, which are the encoding genes in MRCC I, IV and anaerobic respiration, were also significantly decreased in the ruminal epithelium of growth-retarded yaks (p < 0.05). Correlation analysis revealed that the average daily gain (ADG) was significantly positively correlated to the relative mitochondrial DNA copy number (p < 0.01, r = 0.772) and ATP concentration (p < 0.01, r = 0.728) in the ruminal epithelium, respectively. The ruminal weight was positively correlated to the relative mitochondrial DNA copy number (p < 0.05, r = 0.631) and ATP concentration in ruminal epithelium (p < 0.01, r = 0.957), respectively. The ruminal papillae had a significant positive correlation with ATP concentration in ruminal epithelium (p < 0.01, r = 0.770). These results suggested that growth-retarded yaks had a lower VFA metabolism, ketone bodies synthesis, ion absorption, and ATP synthesis in the ruminal epithelium; it also indicated that the growth retardation of yaks is related to the obstruction of cellular ATP synthesis in rumen epithelial cells. [ABSTRACT FROM AUTHOR]

The Regional Veterinary Laboratories in Ireland conducted necropsy examinations on 462 carcasses and 473 fetuses in January 2024, as well as testing 1,226 diagnostic samples. The report provides feedback to practitioners on disease patterns and describes common and unusual cases, with a focus on diseases in food-producing animals. The most common diagnoses in cattle were pneumonia and enteritis, while sheep and goats were commonly diagnosed with fasciolosis (liver fluke infection) and pneumonia. The report also mentions other conditions such as omphalitis, copper poisoning, and laurel poisoning. An image in the report shows pale circular areas on the skin, which may be of interest to those researching dermatological conditions. [Extracted from the article]

Simple Summary: Fetal programming through different maternal nutritional strategies can impact the development of the rumen and cecum in the offspring of Nellore cattle. We evaluated bulls submitted to different prenatal nutrition strategies, namely, non-programming, partial programming, and complete programming. Rumen epithelium was meticulously evaluated for rumenitis and structural irregularities post-slaughter, while cecal lesions were examined after evisceration. Additionally, DNA extraction and the sequencing of distinct amplicon sequence variants in the rumen ecosystem were undertaken. Our metagenomic analysis provided insights into microbial communities influenced by maternal nutrition and dietary factors. This study advances our understanding of fetal programming by emphasizing the intricate interplay between maternal nutrition, gastrointestinal development, and microbial communities. These discoveries contribute significantly to the field of livestock. We explored the influence of maternal nutritional strategies on the development of the rumen and cecum in offspring. Additionally, we investigated the potential repercussions of prenatal nutrition on the rumen and fecal microbiota composition, utilizing metagenomic 16S techniques, to understand the effects of fetal programming (FP) in Nellore cattle. A total of 63 bulls submitted to different prenatal nutrition strategies, namely, non-programming (NP), partial programming (PP), and complete programming (CP), were evaluated. The rumen epithelium was methodically evaluated based on the presence of rumenitis and structural irregularities. The assessment of cecum lesions was conducted post-evisceration, whereby all thoroughly cleaned ceca were methodically evaluated. Samples from 15 animals of rumen fluid at slaughter and feces during the finishing phase were collected, respectively. All DNA extraction were carried out using the Macherey Nagel NucleoSpin Tissue®, and 16S sequencing was conducted using the V4 primers on the MiSeq platform. Within the ruminal ecosystem, an estimated range of 90 to 130 distinct amplicon sequence variants was discerned, as distributed across 45,000 to 70,000 sequencing reads. Our metagenomic exploration unveils microbial communities that distinctly mirror gastrointestinal tract microenvironments and dietary influences. In sum, this comprehensive study advances our comprehension of FP, highlighting the interplay of maternal nutrition, gastrointestinal development, and microbial communities, contributing significantly to the fields of animal science. [ABSTRACT FROM AUTHOR]

Simple Summary: The health status and survival of calves are essential factors affecting the farm's production efficiency and economic efficiency; the main reasons threatening the health of calves are the slow development of the gastrointestinal tract and the low immunity of ruminants at a young age. The coordinated action of the host and its gastrointestinal microflora can improve these negative factors. To better enable the gastrointestinal microflora to play a positive role in the animal organism, the composition and abundance of microorganisms can be regulated in vitro by supplementation with exogenous substances. Oregano essential oil, a natural plant extract, has been reported to be a suitable growth promoter for livestock and poultry, which can promote growth and development, enhance gastrointestinal function, and improve immunity by regulating the gastrointestinal microbiota of ruminants. We investigated oregano essential oil as an exogenous supplement for regulating rumen microbiology in calves and found that it promotes VFA production and can influence calf growth performance and serum antibody levels. This experiment aimed to investigate whether supplementation of calves with different doses of oregano essential oil (OEO) could promote the development of the gastrointestinal tract and enhance the immune ability of calves by regulating the rumen microbiota. Twenty-four 70-day-old healthy and disease-free Holstein male calves were randomly divided into four groups, with the control group fed a basal diet, and the treatment group provided 4 g, 6 g, and 8 g of oregano essential oil per day in addition to the basal diet. After the 14-day pre-test, a 56-day formal test was conducted. At days 0 and 56 of the standard test period, calves were weighed, the average daily weight gain of calves during the test period was calculated, and serum samples were collected to measure the concentration of immunoglobulins (IgA, IgG, and IgM) in the serum; at day 56 of the formal test period, rumen fluid was collected from the calves, and 16SrRNA was sequenced to analyze changes in the rumen microbiota of the calves. The changes in the rumen microbiota of calves were analyzed by 16SrRNA sequencing. The results of the study showed that (1) OEO supplementation in calves significantly increased end weight and average daily gain (p < 0.05); (2) OEO supplementation in calves significantly increased serum concentrations of immunoglobulins IgA and IgM (p < 0.05); (3) OEO supplementation in calves significantly increased the abundance and diversity of rumen microbial organisms (p < 0.05); (4) OEO supplementation in calves significantly regulates the relative abundance of some species, and biomarkers with significant differences were screened by LEfSe analysis: g_Turicibacter, g_Romboutsia, f_Peptostreptococcaceae, f_Clostridiaceae, g_Clostridium_sensu_stricto_1, o_Clostridiales, g_unclassified_f_Synergistaceae, c_Coriobacteriia, o_Coriobacteriales, f_Atopobiaceae, g_Olsenella, p_Actinobacteriota, g_Defluviitaleaceae_UCG-011, f_Defluviitaleaceae, o_Corynebacteriales, g_Corynebacterium, f_Corynebacteriaceae, g_Shuttleworthia, f_Hungateiclostridiaceae, o_norank_c_Clostridia, g_Saccharofermentans, g_Streptococcus, f_Streptococcaceae, g_unclassified_o_Oscillospirales, and f_unclassified_o_Oscillospirales (p < 0.05, LDA ≥ 3); and (5) OEO supplementation in calves significantly enriched the metabolism of cofactors and vitamins pathway (p < 0.05). (6) Using Superman's correlation analysis, we screened unclassified_c_Clostridia, Shuttleworthia, and Christensenellaceae_R-7_group, three beneficial strains for calves. (7) Daily supplementation with 8g of OEO significantly affected rumen microbiota regulation in calves. [ABSTRACT FROM AUTHOR]

Background: Recent data indicated similar growth performance of young calves fed solely high-quality hay instead of a starter diet based on starchy ingredients. Yet, providing exclusively such distinct carbohydrate sources during early life might specifically prime the microbiota and gene expression along the gut of young calves, which remains to be explored. We investigated the effects of starter diets differing in carbohydrate composition, that is medium- or high-quality hay and without or with 70% concentrate supplementation (on fresh matter basis), across the gastrointestinal tract (GIT) of weaned Holstein calves (100 ± 4 days of age) using 16 S rRNA gene sequencing and analyses of short-chain fatty acids and host epithelial gene expressions. Results: The concentrate supplementation drastically decreased microbial diversity throughout the gut, which was also true to a much lesser extent for high-quality hay when compared to medium-quality hay in the foregut. Similarly, the factor concentrate strongly shaped the diet-associated common core microbiota, which was substantially more uniform along the gut with concentrate supplementation. The fermentation profile shifted towards less acetate but more propionate with concentrate supplementation in almost all gut sections, corresponding with higher abundances of starch-utilizing bacteria, while major fibrolytic clusters declined. Noteworthy, the n-butyrate proportion decreased in the rumen and increased in the colon with concentrate, showing an opposite, gut site-dependent effect. Both dietary factors modestly influenced the host epithelial gene expression. Conclusions: Concentrate supplementation clearly primed the microbial ecosystem on a starch-targeted fermentation with characteristic genera occupying this niche along the entire GIT of calves, whereas the microbial differentiation due to hay quality was less distinct. Overall, changes in the microbial ecosystem were only marginally reflected in the targeted transcriptional profile of the host epithelium. [ABSTRACT FROM AUTHOR]

Simple Summary: This study aimed to characterise and compare the development of gut microbiota in dairy calves from birth to weaning, focusing on the impact of a direct-fed microbial (DFM) product containing three strains of lactic acid bacteria (LAB). Forty-four Holstein-Friesian calves were randomly assigned to Treatment (TRT) and Control (CON) groups. TRT calves received a daily dose of the DFM, while CON calves received a placebo and served as the control. Faecal samples and necropsies were collected for analysis. TRT calves exhibited higher live weights at weaning and comparable average daily live weight gain and feed intake to CON calves. TRT calves also demonstrated greater weights of specific gut segments (duodenum, abomasum, reticulum) and enhanced rumen and intestinal development. The microbial diversity was more pronounced in the TRT group, with differences in the relative abundances of eight genera. This study suggests that supplementing with the LAB-based DFM positively influenced calfs' weight, gut development, and microbial diversity. Further research is recommended to explore potential associations between DFM products and gut mucosa-associated microbiota. The calf's gastrointestinal tract (GIT) microbiome undergoes rapid shifts during early post-natal life, which can directly affect calf performance. The objectives of this study were to characterise and compare differences in the establishment and succession of GIT microbiota, GIT morphological changes, and the growth of dairy calves from birth until weaned. Forty-four newborn Holstein-Friesian calves were randomly selected and assigned to Treatment (TRT) and Control (CON) groups. The TRT group calves received a once-daily dose of a direct-fed microbial (DFM) liquid product containing Lacticaseibacillus paracasei, Lentilactobacillus buchneri, and Lacticaseibacillus casei, all formerly known as Lactobacillus. Fresh faecal samples were manually taken from the rectum of all calves, and gross necropsy was performed on the forestomachs and gastrointestinal tracts. Bacterial DNA was extracted from frozen faecal samples for 16S rRNA gene amplicon sequencing. Calves in the TRT group had greater live weights (p = 0.02) at weaning compared with calves in the CON group (mean = 69.18 kg, SD = 13.37 kg). The average daily live weight gain (ADG) and total feed intake were similar between the two groups. Calves in the TRT group had greater duodenum, abomasum, and reticulum weights (p = 0.05). Rumen and intestinal development (p < 0.05) and faecal microbial diversity (p < 0.05) were more pronounced in the TRT group. The relative abundances of eight genera differed (p < 0.001) between the groups. Supplementing calves with the LAB-based DFM increased live weight at weaning and had a more pronounced effect on the development of rumen and the gastrointestinal tract and on microbiota diversity and evenness. Future work is needed to better understand the potential association of LAB-DFM products on gut mucosa-associated microbiota. [ABSTRACT FROM AUTHOR]

Intramuscular fat, also referred to as marbling fat, is the white fat deposited within skeletal muscle tissue. The content of intramuscular fat in the skeletal muscle, particularly the longissimus dorsi muscle, of cattle is a critical determinant of beef quality and value. In this review, we summarize the process of intramuscular fat development and growth, the factors that affect this process, and the molecular and epigenetic mechanisms that mediate this process in cattle. Compared to other species, cattle have a remarkable ability to accumulate intramuscular fat, partly attributed to the abundance of sources of fatty acids for synthesizing triglycerides. Compared to other adipose depots such as subcutaneous fat, intramuscular fat develops later and grows more slowly. The commitment and differentiation of adipose precursor cells into adipocytes as well as the maturation of adipocytes are crucial steps in intramuscular fat development and growth in cattle. Each of these steps is controlled by various factors, underscoring the complexity of the regulatory network governing adipogenesis in the skeletal muscle. These factors include genetics, epigenetics, nutrition (including maternal nutrition), rumen microbiome, vitamins, hormones, weaning age, slaughter age, slaughter weight, and stress. Many of these factors seem to affect intramuscular fat deposition through the transcriptional or epigenetic regulation of genes directly involved in the development and growth of intramuscular fat. A better understanding of the molecular and cellular mechanisms by which intramuscular fat develops and grows in cattle will help us develop more effective strategies to optimize intramuscular fat deposition in cattle, thereby maximizing the quality and value of beef meat. [ABSTRACT FROM AUTHOR]

Introduction: The dietary protein level plays a crucial role in maintaining the equilibrium of rumen microbiota in yaks. To explore the association between dietary protein levels, rumen microbiota, and muscle metabolites, we examined the rumen microbiome and muscle metabolome characteristics in yaks subjected to varying dietary protein levels. Methods: In this study, 36 yaks were randomly assigned to three groups (n = 12 per group): low dietary protein group (LP, 12% protein concentration), medium dietary protein group (MP, 14% protein concentration), and high dietary protein group (HP, 16% protein concentration). Results: 16S rDNA sequencing revealed that the HP group exhibited the highest Chao1 and Observed_species indices, while the LP group demonstrated the lowest. Shannon and Simpson indices were significantly elevated in the MP group relative to the LP group (P < 0.05). At the genus level, the relative abundance of Christensenellaceae_R-7_group in the HP group was notably greater than that in the LP and MP groups (P < 0.05). Conversely, the relative abundance of Rikenellaceae_RC9_gut_group displayed an increasing tendency with escalating feed protein levels. Muscle metabolism analysis revealed that the content of the metabolite Uric acid was significantly higher in the LP group compared to the MP group (P < 0.05). The content of the metabolite L-(+)- Arabinose was significantly increased in the MP group compared to the HP group (P < 0.05), while the content of D-(-)-Glutamine and L-arginine was significantly reduced in the LP group (P < 0.05). The levels of metabolites 13-HPODE, Decanoylcarnitine, Lauric acid, L-(+)-Arabinose, and Uric acid were significantly elevated in the LP group relative to the HP group (P < 0.05). Furthermore, our observations disclosed correlations between rumen microbes and muscle metabolites. The relative abundance of NK4A214_group was negatively correlated with Orlistat concentration; the relative abundance of Christensenellaceae_R-7_group was positively correlated with D-(-)-Glutamine and L-arginine concentrations. Discussion: Our findings offer a foundation for comprehending the rumen microbiome of yaks subjected to different dietary protein levels and the intimately associated metabolic pathways of the yak muscle metabolome. Elucidating the rumen microbiome and muscle metabolome of yaks may facilitate the determination of dietary protein levels. [ABSTRACT FROM AUTHOR]

Background: In this study, we investigated the effects of alpine meadow in different phenological periods on ruminal fermentation, serum biochemical indices, and gastrointestinal tract microbes in grazing yak on the Qinghai-Tibetan Plateau. A total of eighteen female freely grazing yaks with an average age of 3 years old and a body weight of 130 ± 19 kg were selected. According to the plant phenological periods, yaks were randomly allocated to one of three treatments: (1) regreen periods group (RP, n = 6); (2) grassy periods group (GP, n = 6); and (3) hay periods group (HP, n = 6). At the end of the experiment, the blood, rumen fluids, and rectal contents were collected to perform further analysis. Results: The concentrations of total volatile fatty acid (TVFA), acetate, glucose (GLU), triglyceride (TG), cholesterol (CHO), high density lipoprotein (HDL), and low density lipoprotein (LDL) were higher in the GP group than in the HP group (P < 0.05). However, compared with the RP and GP groups, the HP group had higher concentrations of isobutyrate, isovalerate, valerate, and creatinine (CREA) (P < 0.05). The abundance of Prevotella in the rumen, and the abundances of Rikenellaceae_RC9_gut_group, Eubacterium_coprostanoligenes_group, and Prevotellaceae_UCG-004 in the gut were higher in the GP group compared with the HP group (P < 0.05). The HP had higher abundance of Eubacterium_coprostanoligenes_group in the rumen as well as the abundances of Romboutsia and Arthrobacter in the gut compared with the RP and GP groups (P < 0.05). Conclusions: Based on the results of rumen fermentation, serum biochemical, differential biomarkers, and function prediction, the carbohydrate digestion of grazing yak would be higher with the alpine meadow regreen and grassy due to the gastrointestinal tract microbes. However, the risk of microbe disorders and host inflammation in grazing yak were higher with the alpine meadow wither. [ABSTRACT FROM AUTHOR]

Background: Camels are subjected to a wide variety of nutritional deficiencies as they are largely dependent upon grazing desert plants. As a consequence, the syndrome of pica or depraved appetite is occasionally seen in dromedary camels. The condition is manifested as chewing or eating abnormal things such as wood, dirt, bones, stones, clothes, plastics, mud, sand, or other inanimate objects. Aim: This study was designed to investigate the clinical, ultrasonographic, and postmortem findings in dromedary camels with pica or depraved appetite. Methods: Twenty-five camels of 5 days to 15 years were examined. Owner complaints included depraved appetite, loss of body condition, regurgitation of stomach content, and partial or complete absence of feces. Symptoms described were present for a period varying between 3 days, up to 12 months. The stomach compartments and small and large intestines were scanned using ultrasonography from the right and left sides of the abdomen. Necropsy was carried out on six female and three male camels where the thoracic and abdominal organs were examined with special attention to the digestive system. Results: The affected animals had a history of gradual loss of body conditions, eating foreign objects, decreased or total absence of feces, and regurgitation of stomach content. Using ultrasound, the foreign body was imaged occluding completely or partially the intestines. Foreign bodies within the rumen could not be visualized with ultrasound. In cases where the rumen is impacted by sand, small pin-points revealing acoustic enhancement were imaged. Foreign bodies were removed from the rumen at exploratory rumenotomy (n = 11), laparotomy (n = 3), or at necropsy (n = 8) in the form of plastics, cloths, sand, mud, wool balls, robes, glasses, or even metallic objects which may be blunt or sharp. Sixteen (64%) of the camels were recovered while the remaining 9 (36%) did not survive. Conclusion: The syndrome of pica or depraved appetite is an important condition in dromedary resulting in the ingestion of objects other than normal feed. Substantial economic losses are expected as a result of this syndrome. Ultrasonography of the digestive system may help the clinician in some cases to localize of occluding foreign bodies in the intestines, while in the transabdominal scanning of the stomach is valuable only in cases of sand impaction. [ABSTRACT FROM AUTHOR]

The Regional Veterinary Laboratories (RVLs) in Ireland conducted necropsy examinations and diagnostic tests on various animals to identify diseases and conditions. The most common diagnoses in cattle were pneumonia and enteritis, with other cases including parasitic infections and respiratory diseases. The article also discusses diseases and conditions found in bovine foetuses, sheep, and poultry, such as bacterial and fungal pathogens, viral causes, and parasitic gastroenteritis. The report emphasizes the importance of proper management practices and monitoring to prevent the spread of these diseases in livestock. [Extracted from the article]

This document is an editorial discussing the impact of a special issue of the journal Microorganisms titled "The Microbial Population of the Gastrointestinal Tract of Animals: Impacts on Host Physiology." The editorial highlights the increasing interest in studying the microbiome of animals and humans and the complex relationships between microbes and their hosts. The special issue includes research articles and reviews that explore how the microbial population affects the physiology and health of different animal species, including humans. The editorial acknowledges that there is still much to learn about these interactions but emphasizes the potential for future research to improve animal feeding practices and reduce environmental impact while meeting the growing demand for animal protein. [Extracted from the article]

Land snail identification researches in Iraq is limited. In Erbil-Iraq, a study was carried out to explore the identification of various land snails. In earlier studies, most species determination was based on shell morphology, anatomy of genitalia, and mantle color, these criteria are continuously applied to describe new species/new records. Species of similar morphology have ambiguous identities, especially juvenile specimens, due to the great level of morphological variation observed in some snail species. Five species of land snails belong to four families were found in different locations of Erbil City, Iraq, during November 2020 to April 2021, when average temperature was arranged (4-25 C°). Morphology and Cytochrome c oxidase subunit I (COI) gene were used to identify specimens, Rumina decollate (Linnaeus, 1758), found for the first time in Iraq. In addition to four species: Eobania vermiculata (Müller, 1774), Cornu aspersum (Müller, 1774), Cochlicella barbara (Linnaeus, 1758) and Polygyra cereolus (Megerle von Mühlfeldt, 1818) were previously registered in Iraq and have never been recorded in Erbil City, Kurdistan Region, Iraq. This study highlights the impotence of genetic analyses along with morphological analyses in identification of species. This finding suggests, for precise evaluation of species diversity, molecular tools should be included in future biodiversity researches. [ABSTRACT FROM AUTHOR]

[Display omitted] • Mastitis is an inflammatory response in the mammary glands, which brings huge economic loss in the breeding industry. • The occurrence of mastitis is not only related to the infection of mammary gland by external pathogenic microorganisms, but there is also an endogenous pathogenic pathway. • We proposed the pathological concept of "gastroenterogenic mastitis", which is mediated by the gut/rumen-mammary gland axis. • The bacteria/metabolites/neurotransmitters/immune cells translocated from gut/rumen to mammary gland via endogenous pathways such as blood or lymphatic circulation, and mediating the gut/rumen-mammary gland axis. • Targeting gut/rumen microbiota to regulate the gut/rumen-mammary gland axis is a new and effective way to prevent mastitis. Mastitis is an inflammatory response in the mammary gland that results in huge economic losses in the breeding industry. The aetiology of mastitis is complex, and the pathogenesis has not been fully elucidated. It is commonly believed that mastitis is induced by pathogen infection of the mammary gland and induces a local inflammatory response. However, in the clinic, mastitis is often comorbid or secondary to gastric disease, and local control effects targeting the mammary gland are limited. In addition, recent studies have found that the gut/rumen microbiota contributes to the development of mastitis and proposed the gut/rumen–mammary gland axis. Combined with studies indicating that gut/rumen microbiota disturbance can damage the gut mucosa barrier, gut/rumen bacteria and their metabolites can migrate to distal extraintestinal organs. It is believed that the occurrence of mastitis is related not only to the infection of the mammary gland by external pathogenic microorganisms but also to a gastroenterogennic pathogenic pathway. We propose the pathological concept of "gastroenterogennic mastitis" and believe that the gut/rumen–mammary gland axis–mediated pathway is the pathological mechanism of "gastroenterogennic mastitis". To clarify the concept of "gastroenterogennic mastitis" by summarizing reports on the effect of the gut/rumen microbiota on mastitis and the gut/rumen–mammary gland axis–mediated pathway to provide a research basis and direction for further understanding and solving the pathogenesis and difficulties encountered in the prevention of mastitis. [ABSTRACT FROM AUTHOR]

A feeding trial of 120 days was conducted in crossbred calves to examine the impact of feeding varied levels of rumen undegradable protein on growth and economics of production. Eighteen weaned female crossbred calves of age ranging between six to nine months were chosen from University Livestock Farm and Fodder Research and Development Scheme (ULF & FRDS), Mannuthy and randomly allotted to three dietary treatments (CP 20%, TDN 70%) varying in undegradable protein levels: Control- 35%; T1 -45%; T2 -- 55%, respectively of total CP. The experimental calves were given feed as per ICAR standards (ICAR, 2013). The average daily gain (ADG) and total body weight (TWG) were similar between groups. The average feed cost per kg body weight gain was considerably (P<0.05) lower in T1 and T2 groups compared to control. Thus, it was determined that feeding concentrate mixture (20% CP and 70% TDN) with an RUP level of 45 per cent of total CP was economical without affecting the growth rate of calves. [ABSTRACT FROM AUTHOR]

This review contains an analysis of domestic and foreign researchers regarding the occurrence of postpartum complications in cows, which represents a significant veterinary problem. Diagnostic methods and treatment regimens for postpartum pathologies of various origins are presented. Difficulties and errors in diagnosing uterine inflammation lead to improper treatment, prolonged infertility, and significant economic losses. Based on an analysis of the literature, it has been proven that clinical forms of endometritis often develop into a subclinical course. The proportion of hidden endometritis can account for up to 80 % of the total amount of endometrial inflammation. The problem with postpartum complications of the uterus is that the inflammatory processes spread to the ovaries, and this can lead to complete infertility in animals. Rectal examination of cows remains one of the primary methods for diagnosing endometritis. Treatment generally involves the use of a wide range of antibiotics. Data on the hormonal causes of postpartum pathologies of the reproductive system of cows are also presented. A big problem is follicular and luteal cysts, their timely diagnosis and appropriate treatment. The leading cause of cysts is a disruption of the hypothalamic-pituitary-gonadal axis. A practical method for diagnosing cysts is ultrasound, and the type of cyst can be determined by the level of progesterone in the blood. A practical method of treating cysts is the use of hormonal drugs. Data are also presented confirming the significant role of microflora in the occurrence of inflammatory changes in the reproductive organs of cows. The main types of causative agents of inflammatory processes in the genital organs of cows in the postpartum period are given. In cows with endometritis, an imbalance of the uterine microflora is detected, and the growth of bacteria of the genus Bacteroidetes and Fusobacteria with a simultaneous decrease in the number of Proteobacteria and Tenericutes. Data are presented that rumen acidosis can cause inflammation of the uterus, and this is associated with the penetration of Fusobacterium necrophorum into the blood circulation. The effectiveness of intravaginal treatment of cows with a mixture of lactic acid bacteria (L. rhamnosus, P. acidilactici, and L. reuteri) has been shown. [ABSTRACT FROM AUTHOR]

Gene trap locus 2 (GTL2), a long non-coding paternal imprinting gene, participates in various biological processes, including cell proliferation, differentiation, and apoptosis, by regulating the transcription of target mRNA, which is tightly related to the growth of the organic and maintenance of function. In this study, DNA methylation patterns of CpG islands (CGI) of GTL2 were explored, and its expression level was quantified in six tissues, rumen epithelium cells, and skeletal muscle cells in goats. GTL2 expression levels were measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and the methylation model was confirmed by bisulfite-sequencing PCR (BSP). CGI methylation of GTL2 indicated a moderate methylation (ranging from 81.42 to 86.83%) in the brain, heart, liver, kidney, lung, and longissimus dorsi. GTL2 is most highly expressed in brain tissues, but there is no significant difference in the other five tissues. In addition, in the rumen epithelium cell proliferation, GTL2 expression was highest at 60 h, followed by 72 h, and almost unchanged at 12–48 h. In the skeletal muscle cell differentiation, GTL2 expression was highest at 0 and 24 h, significantly decreasing at 72 and 128 h. Pearson correlation analysis did not indicate a clear relationship between methylation and GTL2 expression levels, suggesting that other regulatory factors may modulate GTL2 expression. This study will provide a better understanding of the expression regulation mechanism of genes in the delta-like homolog 1 gene (DLK1)-GTL2 domain. [ABSTRACT FROM AUTHOR]

Increased body growth and feed efficiency are important for breeding due to expensive feed costs. This study estimated the genetic parameters for two feed efficiency traits [the feed conversion ratio (FCR) and the residual feed intake (RFI)] and other important economic traits in male Hu sheep using 1642 male Hu lambs. The heritability of the RFI was estimated at 0.31 (±0.10)–0.54 (±0.1) and the heritability of the FCR was 0.05 (±0.07)–0.77 (±0.12). There was a negative genetic correlation between rib-eye muscle area traits and RFI. A positive genetic correlation was found between fat deposition traits and feed efficiency traits. The digestive tract weight decreased with the direction of RFI: however, the FCR showed the opposite: A low FCR was accompanied by a high digestive tract weight. The genetic correlation between feed efficiency (RFI and FCR) and rumen weight was negative. Heritability estimates for feed efficiency generate fluctuations with different experimental intervals. The weight of other traits should be reasonably allocated to avoid losses in production and feed efficiency in the selection of feed efficiency traits. The present study added to our understanding of the genetic parameters of sheep under the condition of house feeding. [ABSTRACT FROM AUTHOR]

The purpose of this experiment was to study the effects of silage type (ST) and levels of silage particle size (SPS) on feed intake, digestibility, rumen parameters, and feed intake behavior in Kermani sheep. Corn and millet crops were cultivated in May and harvested into coarse and fine forages in mid-September. Four rams (two years old, BW 39.2±3.1 kg) were randomly assigned to four treatments in a 2×2 factorial arrangements in a Latin square design. The experimental diets were coarse corn silage diet; short corn silage diet; coarse millet silage diet and short millet silage diet. Diets were iso-nitrogenous and isoenergetic, and the ratio of forage to concentrate was 50:50. The potential of gas production was higher in corn silage (CS) than millet silage (MS) (P<0.03) and coarse SPS than short SPS (P<0.02). The dry matter (DM), organic matter (OM) and crude protein (CP) intakes were higher in short SPS diets while DM, OM and neutral detergent fiber (NDF) digestibility were higher in CS diets (76.26, 73.43 and 58.71%, respectively), while the CP digestibility was higher in short SPS diets (74.98%). The mean ruminal pH value was lower in CS diets (P<0.05). The mean ruminal ammonia nitrogen (NH3-N) concentration was higher in CS and short SPS diets. Microbial protein (MP) synthesis was higher in short SPS diets (P<0.05). Chewing activity was higher in MS and short SPS diets (P<0.05). The results indicated the higher quality of CS, although MS can be safely fed to small ruminants such. [ABSTRACT FROM AUTHOR]

Ruminants are dependent on their gut microbiomes for nutrient extraction from plant diets. However, knowledge about the composition, diversity, function, and spatial structure of gut microbiomes, especially in wild ruminants, is limited, largely because analysis has been restricted to faeces or the rumen. In two geographically separated reindeer subspecies, 16S rRNA gene amplicon sequencing revealed strong spatial structuring, and pronounced differences in microbial diversity of at least 33 phyla across the stomach, small intestine, and large intestine (including faeces). The main structural feature was the Bacteroidota to Firmicutes ratio, which declined from the stomach to the large intestine, likely reflecting functional adaptation. Metagenome shotgun sequencing also revealed highly significant structuring in the relative occurrence of carbohydrate-active enzymes (CAZymes). CAZymes were enriched in the rumen relative to the small and large intestines. Interestingly, taxonomic diversity was highest in the large intestine, suggesting an important and understudied role for this organ. Despite the two study populations being separated by an ocean and six millennia of evolutionary history, gut microbiome structuring was remarkably consistent. Our study suggests a strong selection for gut microbiome biogeography along the gastrointestinal tract in reindeer subspecies. [ABSTRACT FROM AUTHOR]

The present experiment aimed to compare the effect of the physical form of a starter for dairy replacement calves on some rumen fermentation parameters. Nine male calves 4 days old and averaging 41.3 kg (SD = 3.6) were divided into three groups. The calves were housed in individual hutches bedded with straw. The calves were weaned at the age of 56 days of age and remained in the experiment until they reached 70 days. Three different physical forms of starter feeds were tested: 1) Ground starter -- starter in meal form with coarsely ground maize (GS); 2) Pelleted starter (PS); and 3) Textured starter -- a mixture of whole maize grain plus pelleted protein concentrate (TS). The ingredients and chemical composition of the three starter feeds were similar. The calves from all groups received 4 L of whole milk until 56 days of age. The liquid feed was provided twice daily from 4 to 35 days of age, and once daily from 36 to 56 days. From 35 days of age, calves were offered a free choice of alfalfa hay. OM digestibility was lower in calves that received ground starter compared to calves fed pelleted starter and textured starter. Apparent total tract digestibility of CP was the lowest in calves fed GS compared to those receiving PS and TS (P <0.05). No statistically significant difference in CP digestibility was observed between PS and TS-fed calves. EE digestibility was significantly lower in calves that received GS compared to those that received PS (P <0.05), but not different from EE digestibility in calves fed TS. The highest EE digestibility was that in PS-fed calves. CF digestibility was the highest in calves that received PS and the lowest in those fed GS, with significant differences (P <0.05). There was no significant difference in NFE digestibility among tested physical forms of starter feeds. [ABSTRACT FROM AUTHOR]

This study investigated two outbreaks of spontaneous poisoning by Baccharis coridifolia (Asteraceae) in early-weaned beef calves in Tacuarembó, Uruguay. A total of 34 affected calves showed signs of salivation, anorexia, apathy, marked dehydration, and diarrhea. Deaths occurred 36–72 h after consumption and mortality varied from 37.5% to 43.3% for outbreak 1 and outbreak 2, respectively. The main pathological findings include diffuse severe necrosis of the prestomachs and lymphoid tissues. Ultrastructurally, epithelial cells of the rumen showed swelling, lysis of the organelles, degradation of intercellular attachments, and degradation of the nuclear chromatin. Using LC-MS with diagnostic fragmentation filtering, 56 macrocyclic trichothecenes including glycosyl and malonyl conjugates were identified. The total concentration of macrocyclic trichothecenes, including conjugates, was estimated to be 1.2 ± 0.1 mg/g plant material. This is the first report of these malonyl–glucose conjugates from Baccharis coridifolia. [ABSTRACT FROM AUTHOR]