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Although past studies have included Passiflora among angiosperm lineages with highly rearranged plastid genomes (plastomes), knowledge about plastome organization in the genus is limited. So far only one draft and one complete plastome have been published. Expanded sampling of Passiflora plastomes is needed to understand the extent of the genomic rearrangement in the genus, which is also unusual in having biparental plastid inheritance and plastome‐genome incompatibility. We sequenced 15 Passiflora plastomes using either Illumina paired‐end or shotgun cloning and Sanger sequencing approaches. Assembled plastomes were annotated using Dual Organellar GenoMe Annotator (DOGMA) and tRNAscan‐SE. The Populus trichocarpa plastome was used as a reference to estimate genomic rearrangements in Passiflora by performing whole genome alignment in progressiveMauve. The phylogenetic distribution of rearrangements was plotted on the maximum likelihood tree generated from 64 plastid encoded protein genes. Inverted repeat (IR) expansion/contraction and loss of the two largest hypothetical open reading frames, ycf1 and ycf2, account for most plastome size variation, which ranges from 139 262 base pairs (bp) in P. biflora to 161 494 bp in P. pittieri. Passiflora plastomes have experienced numerous inversions, gene and intron losses along with multiple independent IR expansions and contractions resulting in a distinct organization in each of the three subgenera examined. Each Passiflora subgenus has a unique plastome structure in terms of gene content, order and size. The phylogenetic distribution of rearrangements shows that Passiflora has experienced widespread genomic changes, suggesting that such events may not be reliable phylogenetic markers. [ABSTRACT FROM AUTHOR]

Peroxymonosulfate (PMS) based advanced oxidation processes have gained widespread attention in refractory antibiotics treatment. In this study, Fe 3 O 4 nanoparticles anchored nitrogen-doped porous carbon microspheres (Fe 3 O 4 /NCMS) were synthesized and applied to PMS heterogeneous activation for doxycycline hydrochloride (DOX-H) degradation. Benefitting from synergy effects of porous carbon structure, nitrogen doping, and fine dispersion of Fe 3 O 4 nanoparticles, Fe 3 O 4 /NCMS showed excellent DOX-H degradation efficiency within 20 min via PMS activation. Further reaction mechanisms revealed that the reactive oxygen species including hydroxyl radicals (•OH) and singlet oxygen ( 1 O 2 ) played the dominant role for DOX-H degradation. Moreover, Fe(II)/Fe(III) redox cycle also participated in the radical generation, and nitrogen-doped carbonaceous structures served as the highly active centers for non-radical pathways. The possible degradation pathways and intermediate products accompanying DOX-H degradation were also analyzed in detail. This study provides key insights into the further development of heterogeneous metallic oxides-carbon catalysts for antibiotic-containing wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)