36 results on '"Rabia Mehmood"'
Search Results
2. Synthesis and Evaluation of 1,3,5-Triaryl-2-Pyrazoline Derivatives as Potent Dual Inhibitors of Urease and α-Glucosidase Together with Their Cytotoxic, Molecular Modeling and Drug-Likeness Studies
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Rabia Mehmood, Amina Sadiq, Reem I. Alsantali, Ehsan Ullah Mughal, Meshari A. Alsharif, Nafeesa Naeem, Asif Javid, Munirah M. Al-Rooqi, Gul-e-Saba Chaudhry, and Saleh A. Ahmed
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Chemistry ,QD1-999 - Published
- 2022
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3. Psychological impacts of COVID-19 and satisfaction from online classes: disturbance in daily routine and prevalence of depression, stress, and anxiety among students of Pakistan
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Muhammad Babar Khawar, Muddasir Hassan Abbasi, Shabbir Hussain, Mehwish Riaz, Mussarat Rafiq, Rabia Mehmood, Nadeem Sheikh, Hafiza Nabeela Amaan, Sana Fatima, Faiza Jabeen, Zaira Ahmad, and Adil Farooq
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COVID-19 ,Pandemic ,Panic ,Stress management ,Psychological impact ,Students ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The present study investigated the (i) socio-demographic predictors of psychological distress, (ii) socio-demographic predictors of satisfaction from online classes, and (iii) the relationship between psychological distress and satisfaction from online classes among university students of Pakistan during the COVID-19 pandemic. An online questionnaire-based survey was conducted. A total of 2220 respondents that was enrolled at the University of the Punjab (PU), University of Management and Technology (UMT), and the University of Central Punjab (UCP) were involved in the current study. Data were collected at a 64% response rate and analyzed with SPSS IBM Version 21.0. Results revealed that approximately 41% of the students were facing severe psychological distress while about 65% were found unsatisfied with online classes. Besides, a linear negative relationship between the independent variable, i.e. psychological distress and the dependent variable, i.e. satisfaction from online classes was found. Therefore, to minimize the level of psychological distress and increase students’ satisfaction with online classes it is highly recommended to take precautionary measures by the relevant stakeholders.
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- 2021
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4. Alkalophilic Protease Producing Bacteria and Some Biotechnological Potentials
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Muddasir Hassan Abbasi, Rabia Mehmood, Muhammad Babar Khawar, Hafiza Nabeela Amaan, Amin Arif, Tahaa Saeed, and Mussarat Rafiq
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Alkalophilic, Biotechnological potential, Extremophiles, Industries, Protease. ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background: Extremophiles are a hot topic in the field of biotechnology for their immense potential and applications in multiple industries. Objectives: The present review aims to sum up the potential applications of alkalophilic protease-producing bacteria and their optimized growth requirement. The isolation, characterization, and optimization of various isolates (especially of genus Bacillus) from different harsh niches, including soil samples from deserts and soil having decaying matters, wastewaters from industries, soda lakes, and alkaline springs have been reported in this review. Methodology: All the relevant papers published from 2013-2020 were looked over numerous sources like Google Scholar, Medline, PubMed, Research Gate, Science Direct, Scopus and Web of Science. Results: Most of the microbial life found in extreme alkaline habitats are found to form a variety of enzymes and an array of other substances of biotechnological interests. These enzymes, especially proteases, are exploited in industries globally because of their ability to withstand rigorous industrial reactions and conditions. Conclusion: Though a number of alkalophilic protease-producing bacteria have been isolated, still a large number of these micro-organisms are unidentified. The current demand for biotechnological products from them appeals to the need for isolation of unidentified bacteria.
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- 2021
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5. Assessing the Hepatotoxicity of Industrial Leachate; Histopathology and Heavy Metal Contents in Liver of Wistar Rats
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Muhammad Babar Khawar, Rabia Mehmood, Muddasir Hassan Abbasi, and Nadeem Sheikh
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Heavy metals ,Histopathology ,Leachate ,Toxicity ,Wastewater ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
The process of paper production requires a huge quantity of water and energy and in turn contributes a number of effluents in the form of phenolics, toxic organic compounds and heavy metals in wastewater (leachate). The present investigation was aimed to assess the toxic effects of leachate on liver micro-architecture and heavy metal elements of the liver. Eighteen (18) healthy male Wistar rats (240 ± 10g) were selected and acclimatized prior to experimental treatment. These rats were randomly divided into three groups viz, Control group (received 4ml/ kg normal saline), Group 1 (4ml/ kg leachate) and Group 2 (4ml/ kg 1:10 diluted leachate). All the animals were dissected and liver tissues were collected and processed accordingly after 24 h of leachate treatment. High level of cadmium and chromium were found in Group 1 as compared to the control group upon liver metal contents analysis found out by flame atomic absorption spectrometer. A clear disruption of micro-architecture of the liver, congested sinusoids, damaged central vein, and perturbed morphology was observed in Group-1 as revealed by H & E staining. Moreover, loss of polarity, congestion, and disruption of hepatocytes and pronounced vacuolization in the cytoplasm was observed in Group 2 compared to control sections. On the basis of above findings, it can be concluded that paper industry leachate is highly toxic and its intraperitoneal injection results in hepatotoxicity that not only affects the hepatic micro-architecture but also results in perturbed liver metal contents. Therefore, proper treatment of such wastewater is required before its disposal.
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- 2018
6. A novel single nucleotide polymorphism in exon 3 of MYOC enhances the risk of glaucoma.
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Sabeen Nazir, Maryam Mukhtar, Maryam Shahnawaz, Shaima Farooqi, Naz Fatima, Rabia Mehmood, and Nadeem Sheikh
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Medicine ,Science - Abstract
Genetic polymorphismsof MYOCalter the myocilin protein,which leads to disruption of thenormal regulation of intraocular pressure (IOP) that ultimately causes glaucoma.Theaim of the present study was to identify the polymorphism in exon 3 of the MYOC gene of theglaucoma patients in Lahore, Pakistan. We conducted a case-control study with 100 patients and 100 controls subjects. We extracted DNA from blood samples,amplified the target DNA fragmentby PCR, and identifiedpolymorphisms through sequencing. We observed that the allelic and genotypic frequencies of rs74315341 and rs879255525 were associated with glaucoma in our patient population. The polymorphism atrs74315341 led to the substitutionof serine for arginine,whereas the polymorphism at rs879255525 led to the substitution ofasparagine for lysine. The haplotype TGAAGCCATTTC was associated with disease onset, whereas the haplotype GGAAGCCATTTC was protective against disease development. In conclusion, weidentified MYOC gene polymorphisms in susceptible regions that were associated withglaucoma onset among the Lahore patient population.This is the first report to identify a novel mutation in rs879255525 in exon 3 of the MYOC genethat is associated withglaucoma.
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- 2018
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7. Common Problems in Software Requirement Engineering Process: An Overview of Pakistani Software Industry.
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Sadia Khalid, Uzair Rasheed, Mishal Muneer, Wasi Haider Butt, Rabia Mehmood, and Usman Qamar
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- 2023
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8. Therapeutic Potential of Microbial Metabolites: New Insights and Perspectives
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Muhammad Idnan, Rabia Mehmood, Muhammad Ahsan Ashraf, Adil Farooq, Ume Habiba, Syeda Eisha Hamid, Sara Shahzaman, Maryam Mukhtar, Nimra Afzal, Nadeem Sheikh, Muddasir Hassan Abbasi, Nayab Shahid, Ali Afzal, Rimsha Naseem, Muhammad Babar Khawar, and Muhammad Abu Talha Safdar Hashmi
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General Biochemistry, Genetics and Molecular Biology - Published
- 2023
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9. Anemia among pregnant women a major concern for achieving universal health coverage
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null Asma Abdul Qadeer, null Rabia Mehmood, null Saadia Baraan, null Nadia Junaid, null Sara Bashir Kant, and null Sarah Habib
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Forestry ,Plant Science - Abstract
Objective: To assess the frequency of anemia among pregnant females visiting Rawal Institute of Health Sciences and to find out the risk factors contributing to anemia. Study Design: Cross Sectional Descriptive study. Setting: Rawal Institute of Health Sciences, Islamabad, Pakistan. Period: May to July 2019. Material & Methods: A study was carried out to find the frequency of anemia among 100 pregnant women through non-probability convenient sampling at RIHS using a structured questionnaire. Hemoglobin concentration data in the blood was collected from their antenatal archives. Results: Hemoglobin level was found to be less than 7 g/dl in 3% of the pregnant females and 6% had moderate anemia. In addition to that 68% were mildly anemic. Overall frequency of anemic pregnant women was found to be 77%. Conclusion: In conclusion, anemia in this study population was high frequency. This high frequency according to our study is related to inadequate diet, stress, multiple pregnancies and menorrhagia.
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- 2023
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10. Neutrophil-to-lymphocyte ratio as a promising biomarker for the diagnosis of psoriasis in Pakistan
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Rabia Mehmood, Fareeha Azam, Mussarat Rafiq, Syed Shakeel Shah, Nadeem Sheikh, Muddasir Hassan Abbasi, Syeda Eisha Hamid, Suneela Aman, Ali Afzal, Muhammad Babar Khawar, and Benish Nawaz
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General Biochemistry, Genetics and Molecular Biology - Published
- 2022
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11. Synthesis of Novel 2,3-Dihydro-1,5-Benzothiazepines as α-Glucosidase Inhibitors: In Vitro, In Vivo, Kinetic, SAR, Molecular Docking, and QSAR Studies
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Rabia Mehmood, Ehsan Ullah Mughal, Eslam B. Elkaeed, Rami J. Obaid, Yasir Nazir, Hanan A. Al-Ghulikah, Nafeesa Naeem, Munirah M. Al-Rooqi, Saleh A. Ahmed, Syed Wadood Ali Shah, and Amina Sadiq
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General Chemical Engineering ,General Chemistry - Published
- 2022
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12. Overview of Infectious Anemia In chicken and Role of PCR in disease Diagnose
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Ayesha Muazzam and Rabia Mehmood
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Chicken Infectious Anemia is a viral disease in poultry caused by CAV chicken Anemia virus usually effecting chicken and other species of poultry birds. This review will show the role of PCR which is a device used to diagnose disease. In this review I also brief some sign and symptom of the disease which will help a lay men to diagnose the disease. In this review I also discuss the control method of disease by which we can control the disease and save the farmer from big loss. The objective of that review was to aware the people from risk of CIA and give possible solution to combat with disease. The review will help out scholars to understand CIA. This review also bring light toward the use of PCR as in Pakistan farmer are not using PCR and in result there is big difficulty in diagnose of disease. This study will also aware about use of vaccine to the people so people will know the importance of Vaccine.
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- 2022
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13. Dichotomous role of autophagy in cancer
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Amin Arif, Muhammad Babar Khawar, Rabia Mehmood, Muddasir Hassan Abbasi, and Nadeem Sheikh
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Geography, Planning and Development ,Management, Monitoring, Policy and Law - Abstract
Autophagy is an evolutionary conserved catabolic process that plays physiological and pathological roles in a cell. Its effect on cellular metabolism, the proteome, and the number and quality of organelles, diversely holds the potential to alter cellular functions. It acts paradoxically in cancer as a tumor inhibitor as well as a tumor promoter. In the early stage of tumorigenesis, it prevents tumor initiation by the so-called “quality control mechanism” and suppresses cancer progression. For late-staged tumors that are exposed to stress, it acts as a vibrant process of degradation and recycling that promotes cancer by facilitating metastasis. Despite this dichotomy, the crucial role of autophagy is evident in cancer, and associated with mammalian targets of rapamycin (mTOR), p53, and Ras-derived major cancer networks. Irrespective of the controversy regarding autophagic manipulation, promotion and suppression of autophagy act as potential therapeutic targets in cancer treatment and may provide various anticancer therapies.
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- 2022
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14. Metal Dust Exposure Caused Changes in Blood Indices and Serum Proteins
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Rabia Mehmood and Nadeem Sheikh
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Background: Amongst the industrial hubs in Pakistan, Wazirabad is renowned for its cutlery industry. Cutlery industry generates heaps of multi-metallic dust in whetting units during the processing of stain fewer steel tools. This dust comprises certain potentially toxic and even carcinogenic constituents, thus pose a serious health threat to the workers involved in its processing. Laborers health and safety is something quite non-seriously considered in most of the developing countries, no different is Pakistan. Present exploration was aimed at searching for the differences, in blood profile and quantitative serum protein profile of a group of laborers in cutlery industry that are directly and regularly exposed to multi-metallic dust. Materials and Methods: After taking written consent from the participants, blood samples were drawn for hematological analysis and serum analysis. Hematological analysis was performed with hematological analyzer and serum was subjected to SDS gel electrophoresis for protein profiling. Results: Statistically significant changes were observed in the number of RBCs, MCV, HCT and RDW, whereas platelet count was decreased in experimental groups when compared to control group. Serum protein profiling using SDS-PAGE revealed the protein fractions ranging from 73 to 287 kDa. Densitometric analysis has shown changes in the serum proteins of the subjects exposed to metal dust. Conclusion: Chronic exposure to the metal dust induce changes in the hematological parameters as well as serum proteins. The industrial workers should ensure the use of industry specific personal safety equipment. Key words: Health hazard, Hematology, Metals, Metal dust, Proteins, SDS-PAGE.
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- 2022
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15. Novel functional polymorphism on PADI-4 gene and its association with arthritis onset
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Naz Fatima, Andleeb Batool, Muhammad Babar Khawar, Nadeem Sheikh, Rabia Mehmood, and Maryam Mukhtar Dr
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Genetics ,Genotyping ,QH301-705.5 ,Haplotype ,Citrullination ,Arthritis ,Single-nucleotide polymorphism ,Biology ,medicine.disease ,Osteoarthritis ,Genotype ,medicine ,PADI-4 gene polymorphism ,Gene polymorphism ,Rheumatoid arthritis ,Biology (General) ,Restriction fragment length polymorphism ,General Agricultural and Biological Sciences - Abstract
Background Citrullinated proteins formed by peptidyl arginine deiminases (PADIs) deimination of arginine residues in proteins are of particular interest in arthritis pathogenesis. Polymorphisms on the PADI-4 gene lead to the malfunctioning of PADIs leading to the onset of arthritis. Objective The present study was conducted to determine the polymorphisms on the PADI-4 gene and their association with rheumatoid arthritis (RA) as well as Osteoarthritis (OA). Methodology To achieve the above-mentioned objective a case-control study was conducted. Blood samples were collected from RA, OA, and control subjects. DNA was extracted from each blood sample by modified organic method and was quantified as well as qualified by DNA gel electrophoresis and Nanodrop. Patients were tested for rs874881, rs11203366, rs11203367, rs2240336, rs2240337, rs2240339, rs1748033 and rs2240340 polymorphic sites by amplifying targeted regions through PCR with site-specific primers. Genotyping was performed by Restriction Fragment Length Polymorphism and direct sequencing method. Mutations were identified by analyzing sequences on BioEdit software. Allelic, genetic, and multiple site analysis were performed by SHEsis and PLINK software. Change in the amino acid sequence was identified by MEGA 6.0 software. Results Polymorphisms were identified on all targeted polymorphic sites except rs2240337 in both RA and OA individuals. In addition, two novel mutations were also identified in exon 4 identified i-e SCV000804840: c.218T > C and SCV000807675: c.241G > T. All the SNPs except rs11203366 were found to be significantly associated with RA at an allelic level whereas all SNP’s have been significant risk factors in the onset of OA. At genotypic level rs874881, rs11203366, rs2240339, SCV000804840 and SCV000807675 were significantly associated to RA development whereas rs874881, rs11203366, rs11203367, rs2240339, SCV000804840 and SCV000807675 were genetic risk factors in OA onset. Haplotype analysis indicated that GACCACGCC and GACCACGCT were highly significant in disease development. Polymorphisms identified altered the functioning of PADIs by altering their amino acid sequence. Conclusion In conclusion, it was found that PADI-4 gene polymorphism was not only involved in the onset of RA but was also found to be a significant risk factor in OA onset.
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- 2022
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16. Obesity, A Preventable Burden of Disease among Children
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Dr. Asma Abdul Qadeer, Dr. Rabia Mehmood, Dr. Nadia Junaid, Dr. Sara Bashir, Dr. Saadia Baran, and Dr. Saira Mahmood
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General Medicine - Abstract
Background: In last few decades, childhood obesity has become a burdensome challenge globally. The most important long-term consequence of childhood obesity is its persistence into adulthood, with all the associated health risks. Persistent obesity is established before the age of 11. Methods: A descriptive cross sectional study with non-probability convenient sampling was carried out over a period of three month in a private sector school Islamabad, Pakistan. The sampling frame was 250 students of grade 4 of roots school system. The response rate was 67%. Results: Mean age in our sample was 10 years. Mean BMI was 18.14 with a standard deviation of 4.4. The frequency of obesity in children was 22% (more than 97th percentile and equivalent to BMI of 30kg/m 2) and that of overweight was 32% (more than 85th percentile and BMI of). Among obese children 28% were reasonably physically active the past week. Regarding eating habits 52% children ate their breakfast every day, 16% of the children never had their breakfast because they didn’t like breakfast or because they were never given breakfast at home. 55% of the children thought that obesity can be prevented by making sure that children are into sports and games, by promoting healthy snacks and banning fast foods, by launching fitness programs and by health education. Conclusion: We found a high frequency of obese and overweight children in the private sector school of Islamabad. Physical inactivity and relationship with other factors like watching TV, gaming and over consumption of junk food was found to be associated with overweight and obesity adds some useful data to the previous researches.
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- 2022
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17. A Decade of Mighty Lipophagy: What We Know and What Facts We Need to Know?
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Naila Naz, Rabia Mehmood, Muhammad Babar Khawar, Nadeem Sheikh, Mussarat Rafiq, and Muddasir Hassan Abbasi
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Metabolic Syndrome ,Aging ,Programmed cell death ,Cell signaling ,QH573-671 ,Autophagy ,Biological membrane ,Review Article ,Lipid Droplets ,Cell Biology ,General Medicine ,Biology ,Lipid Metabolism ,Biochemistry ,Cell biology ,Lipotoxicity ,Lipid droplet ,Animals ,Humans ,Obesity ,Cytology ,Homeostasis ,Function (biology) - Abstract
Lipids are integral cellular components that act as substrates for energy provision, signaling molecules, and essential constituents of biological membranes along with a variety of other biological functions. Despite their significance, lipid accumulation may result in lipotoxicity, impair autophagy, and lysosomal function that may lead to certain diseases and metabolic syndromes like obesity and even cell death. Therefore, these lipids are continuously recycled and redistributed by the process of selective autophagy specifically termed as lipophagy. This selective form of autophagy employs lysosomes for the maintenance of cellular lipid homeostasis. In this review, we have reviewed the current literature about how lipid droplets (LDs) are recruited towards lysosomes, cross-talk between a variety of autophagy receptors present on LD surface and lysosomes, and lipid hydrolysis by lysosomal enzymes. In addition to it, we have tried to answer most of the possible questions related to lipophagy regulation at different levels. Moreover, in the last part of this review, we have discussed some of the pathological states due to the accumulation of these LDs and their possible treatments under the light of currently available findings.
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- 2021
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18. Metagenome‐Guided Analogue Synthesis Yields Improved Gram‐Negative‐Active Albicidin‐ and Cystobactamid‐Type Antibiotics
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Melinda A. Ternei, Zongqiang Wang, Rabia Mehmood, Amanda Kasper, Sean F. Brady, Shao-Gang Li, and Joel S. Freundlich
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chemistry.chemical_classification ,Biological Products ,Xanthomonas ,Molecular Structure ,medicine.drug_class ,Chemistry ,Antibiotics ,General Medicine ,General Chemistry ,Article ,Catalysis ,Endopeptidase ,Anti-Bacterial Agents ,Analogue synthesis ,Enzyme ,Biochemistry ,Metagenomics ,medicine ,Organic Chemicals ,4-Aminobenzoic Acid ,Gene ,Adenylylation ,Gram - Abstract
Natural products are a major source of new antibiotics. Here we utilize biosynthetic instructions contained within metagenome-derived congener biosynthetic gene clusters (BGCs) to guide the synthesis of improved antibiotic analogues. Albicidin and cystobactamid are the first members of a new class of broad-spectrum ρ-aminobenzoic acid (PABA)-based antibiotics. Our search for PABA-specific adenylation domain sequences in soil metagenomes revealed that BGCs in this family are common in nature. Twelve BGCs that were bio-informatically predicted to encode six new congeners were recovered from soil metagenomic libraries. Synthesis of these six predicted structures led to the identification of potent antibiotics with changes in their spectrum of activity and the ability to circumvent resistance conferred by endopeptidase cleavage enzymes.
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- 2021
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19. High-fat diet-induced splenic, hepatic, and skeletal muscle architecture damage: cellular and molecular players
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Tasleem Akhtar, Rabia Mehmood, Nadeem Sheikh, Tayyeba Batool, Muhammad Babar Khawar, Sania Nadeem, and Ambreen Asghar
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0301 basic medicine ,medicine.medical_specialty ,Necrosis ,business.industry ,Clinical Biochemistry ,Skeletal muscle ,Spleen ,Physical exercise ,Inflammation ,Cell Biology ,General Medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,Immune system ,030220 oncology & carcinogenesis ,Hemosiderin ,Internal medicine ,Red pulp ,Medicine ,medicine.symptom ,business ,Molecular Biology - Abstract
The trend of consuming food high in calories, fat, and sugar with little nutritional value and reduced physical exercise has resulted in an alarming ratio of overweight and obese subjects worldwide. Low-grade chronic inflammation is the key feature of obesity that causes an increase in pro-inflammatory cytokines and decrease in anti-inflammatory cytokines in circulation. The current study was aimed to investigate the effect of high-fat diet on the architecture of spleen, liver, and skeletal muscle and changes in the expression of hepatic cytokines. Two groups of experimental rats were established, against control that were given different percentage of fats in their diet. After a period of sixteen weeks, rats were dissected and their organs were excised out and processed accordingly. Spleen sections of experimental groups, revealed increased recruitment of lymphocytes, sinusoidal dilatations, necrotic lymphocytes, increased ratio of white-to-red pulp, and hemosiderin and iron deposits in red pulp indicating immune system activation. Hepatic sections showed enlarged sinusoidal spaces, disruptive hepatocytes, necrosis and dilation of portal veins. Sections of skeletal muscle showed degenerating fibers, increased fat accumulation, and recruitment of macrophages. Elevated expression of IFN-γ and decreased expression of IFN-α and IFN-β cytokines verified the adverse effect of high-fat diet on immune system as well. Fats tend to accumulate in organs due to increased intake of fat-rich diet disturbing their normal function and histology. In addition, gene expression analysis of cytokines confirmed the effect of high-fat diet as an inflammatory agent.
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- 2021
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20. Variations in Peripheral Hematological Parameters as a Diagnostic Biomarker of HBV Infection
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Nimra Afzal, Rabia Mehmood, Fareeha Azam, Mussarat Rafiq, Nadeem Sheikh, Muddasir Hassan Abbasi, Syeda Eisha Hamid, Ali Afzal, Syed Shakeel Shah, Suneela Aman, Ume Habiba, Muhammad Babar Khawar, and Huma Rasheed
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Fuel Technology ,Energy Engineering and Power Technology - Published
- 2022
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21. Novel functional polymorphism on
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Maryam, Mukhtar, Nadeem, Sheikh, Andleeb, Batool, Muhammad Babar, Khawar, Naz, Fatima, and Rabia, Mehmood
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Citrullinated proteins formed by peptidyl arginine deiminases (PADIs) deimination of arginine residues in proteins are of particular interest in arthritis pathogenesis. Polymorphisms on theThe present study was conducted to determine the polymorphisms on theTo achieve the above-mentioned objective a case-control study was conducted. Blood samples were collected from RA, OA, and control subjects. DNA was extracted from each blood sample by modified organic method and was quantified as well as qualified by DNA gel electrophoresis and Nanodrop. Patients were tested for rs874881, rs11203366, rs11203367, rs2240336, rs2240337, rs2240339, rs1748033 and rs2240340 polymorphic sites by amplifying targeted regions through PCR with site-specific primers. Genotyping was performed by Restriction Fragment Length Polymorphism and direct sequencing method. Mutations were identified by analyzing sequences on BioEdit software. Allelic, genetic, and multiple site analysis were performed by SHEsis and PLINK software. Change in the amino acid sequence was identified by MEGA 6.0 software.Polymorphisms were identified on all targeted polymorphic sites except rs2240337 in both RA and OA individuals. In addition, two novel mutations were also identified in exon 4 identified i-e SCV000804840: c.218T C and SCV000807675: c.241G T. All the SNPs except rs11203366 were found to be significantly associated with RA at an allelic level whereas all SNP's have been significant risk factors in the onset of OA. At genotypic level rs874881, rs11203366, rs2240339, SCV000804840 and SCV000807675 were significantly associated to RA development whereas rs874881, rs11203366, rs11203367, rs2240339, SCV000804840 and SCV000807675 were genetic risk factors in OA onset. Haplotype analysis indicated that GACCACGCC and GACCACGCT were highly significant in disease development. Polymorphisms identified altered the functioning of PADIs by altering their amino acid sequence.In conclusion, it was found that
- Published
- 2021
22. Role and Molecular Mechanisms of Lysosomes and Cathepsins in Neuropathology and Aging: New Insights
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Muhammad Babar, Khawar, primary, Naz, Fatima, additional, Muddasir Hassan, Abbasi, additional, Nadeem, Sheikh, additional, Hafiza Nabeela, Amaan, additional, Rabia, Mehmood, additional, Maryam, Mukhtar, additional, Amin, Arif, additional, Zaira, Ahmad, additional, Zerwa, Siddique, additional, Adil, Farooq, additional, and Tahaa, Saeed, additional
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- 2021
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23. Modification of Emulsifying Properties of Cereal Flours by Blending with Legume Flours
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Momna Aslam, Tanzila Rehman, Haq Nawaz, and Rabia Mehmood
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food.ingredient ,food ,Rapeseed ,Chemistry ,Wheat flour ,General Medicine ,Food science ,Canola ,Sunflower ,Legume - Abstract
Background: The effect of blend formation on emulsifying activity (EA) and emulsifying stability (ES) of some commonly used cereal and legume flours, using different oils, was studied. Methods: The blends of wheat flour (WF), refined wheat flour (RWF) and maize flour (MF) were prepared by mixing with equal proportions of chickpea flour (CPF) (1:1w/w) in a kitchen blender and analyzed for EA and ES using coconut, canola, corn, rapeseed and sunflower oils. Result: Statistically significant variations (p less than 0.05) were observed in the emulsifying properties of the flours and their blends. The blending of cereal flours with CPF showed mixed responses of variation in emulsifying properties from those of the respective pure flours. The EA of the blends was found to be increased from those of the pure WF and RWF but decreased from those of the pure MF and CPF with some exceptions. However, the ES of the blends was decreased from that of the pure WF and increased from those of the pure RWF, MF and CPF. The data would be a valuable contribution to the literature regarding the improvement of functional properties of cereal foods.
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- 2021
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24. Psychological impacts of COVID-19 and satisfaction from online classes: disturbance in daily routine and prevalence of depression, stress, and anxiety among students of Pakistan
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Adil Farooq, Rabia Mehmood, Mehwish Riaz, Nadeem Sheikh, Zaira Ahmad, Faiza Jabeen, Shabbir Hussain, Muhammad Babar Khawar, Sana Fatima, Hafiza Nabeela Amaan, Mussarat Rafiq, and Muddasir Hassan Abbasi
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0301 basic medicine ,Stress management ,Science (General) ,education ,Computer-assisted web interviewing ,03 medical and health sciences ,Q1-390 ,0302 clinical medicine ,Stress (linguistics) ,medicine ,Students ,Psychological impact ,Depression (differential diagnoses) ,Response rate (survey) ,H1-99 ,Multidisciplinary ,Pandemic ,Panic ,COVID-19 ,Social sciences (General) ,030104 developmental biology ,Negative relationship ,Anxiety ,medicine.symptom ,Psychology ,030217 neurology & neurosurgery ,Clinical psychology ,Research Article - Abstract
The present study investigated the (i) socio-demographic predictors of psychological distress, (ii) socio-demographic predictors of satisfaction from online classes, and (iii) the relationship between psychological distress and satisfaction from online classes among university students of Pakistan during the COVID-19 pandemic. An online questionnaire-based survey was conducted. A total of 2220 respondents that was enrolled at the University of the Punjab (PU), University of Management and Technology (UMT), and the University of Central Punjab (UCP) were involved in the current study. Data were collected at a 64% response rate and analyzed with SPSS IBM Version 21.0. Results revealed that approximately 41% of the students were facing severe psychological distress while about 65% were found unsatisfied with online classes. Besides, a linear negative relationship between the independent variable, i.e. psychological distress and the dependent variable, i.e. satisfaction from online classes was found. Therefore, to minimize the level of psychological distress and increase students’ satisfaction with online classes it is highly recommended to take precautionary measures by the relevant stakeholders., COVID-19, Pandemic, Panic, Stress management, Psychological impact, Students.
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- 2021
25. Genetic Susceptibility Of Cytotoxic T Lymphocyte-Associated Antigen 4 Gene Polymorphism In The Onset Of Arthritis
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Saira Kainat Suqaina, Tayyaba Saleem, Rabia Mehmood, Nadeem Sheikh, Muhammad Babar Khawar, and Maryam Mukhtar
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Polymorphism (computer science) ,Genetic marker ,Haplotype ,Immunology ,Genetic predisposition ,medicine ,Arthritis ,Gene polymorphism ,Biology ,Allele ,medicine.disease ,Genotyping - Abstract
Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) gene plays a vital role in the activation of T-cells as a down regulator. CTLA-4 gene polymorphisms have implicated a potential risk factor for autoimmune disorders like arthritis. Therefore the current study was designed to determine the association of CTLA-4 gene polymorphism in the onset of rheumatoid and osteoarthritis in Pakistani individuals. Genotyping was performed on 300 RA, 316 OA, and 412 control subjects by direct sequencing method as well as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. It was observed that allelic and genotypic frequency of rs5742909, rs231775, rs4553808, rs733618, and rs3087243 were significantly varied among patients and controls and considered as a significant risk factor in the onset of RA as well as OA. However, no mutation was identified on the rs11571317 polymorphic site. Haplotype CAGTCA and CAG TCG act as a protectant against disease onset whereas CAACCG was significant in disease onset. Mutation on rs231775 polymorphic site lead to the change of threonine into alanine It was concluded that CTLA-4 gene polymorphism is a significant risk factor in the onset of RA as well as OA. Large scale survey is required for the screening of the genetic markers for pre-diagnosis of the disease.SUMMARY STATEMENTThe study summarized that CTLA-4 gene polymorphism plays a key role in the arthritis onset in Pakistani population.
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- 2021
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26. High-fat diet-induced splenic, hepatic, and skeletal muscle architecture damage: cellular and molecular players
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Ambreen, Asghar, Tasleem, Akhtar, Tayyeba, Batool, Muhammad Babar, Khawar, Sania, Nadeem, Rabia, Mehmood, and Nadeem, Sheikh
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Male ,Liver ,Macrophages ,Animals ,Interferons ,Obesity ,Rats, Wistar ,Diet, High-Fat ,Muscle, Skeletal ,Spleen ,Rats - Abstract
The trend of consuming food high in calories, fat, and sugar with little nutritional value and reduced physical exercise has resulted in an alarming ratio of overweight and obese subjects worldwide. Low-grade chronic inflammation is the key feature of obesity that causes an increase in pro-inflammatory cytokines and decrease in anti-inflammatory cytokines in circulation. The current study was aimed to investigate the effect of high-fat diet on the architecture of spleen, liver, and skeletal muscle and changes in the expression of hepatic cytokines. Two groups of experimental rats were established, against control that were given different percentage of fats in their diet. After a period of sixteen weeks, rats were dissected and their organs were excised out and processed accordingly. Spleen sections of experimental groups, revealed increased recruitment of lymphocytes, sinusoidal dilatations, necrotic lymphocytes, increased ratio of white-to-red pulp, and hemosiderin and iron deposits in red pulp indicating immune system activation. Hepatic sections showed enlarged sinusoidal spaces, disruptive hepatocytes, necrosis and dilation of portal veins. Sections of skeletal muscle showed degenerating fibers, increased fat accumulation, and recruitment of macrophages. Elevated expression of IFN-γ and decreased expression of IFN-α and IFN-β cytokines verified the adverse effect of high-fat diet on immune system as well. Fats tend to accumulate in organs due to increased intake of fat-rich diet disturbing their normal function and histology. In addition, gene expression analysis of cytokines confirmed the effect of high-fat diet as an inflammatory agent.
- Published
- 2021
27. Alkalophilic Protease Producing Bacteria and Some Biotechnological Potentials
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Tahaa Saeed, Hafiza Nabeela Amaan, Rabia Mehmood, Amin Arif, Muddasir Hassan Abbasi, Mussarat Rafiq, and Muhammad Babar Khawar
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Protease ,Genus Bacillus ,Web of science ,biology ,business.industry ,medicine.medical_treatment ,lcsh:R ,lcsh:Medicine ,Isolation (microbiology) ,biology.organism_classification ,Alkalophilic, Biotechnological potential, Extremophiles, Industries, Protease ,Biotechnology ,lcsh:Biology (General) ,medicine ,General Earth and Planetary Sciences ,Extremophile ,business ,lcsh:QH301-705.5 ,Bacteria ,General Environmental Science - Abstract
Background: Extremophiles are a hot topic in the field of biotechnology for their immense potential and applications in multiple industries. Objectives: The present review aims to sum up the potential applications of alkalophilic protease-producing bacteria and their optimized growth requirement. The isolation, characterization, and optimization of various isolates (especially of genus Bacillus) from different harsh niches, including soil samples from deserts and soil having decaying matters, wastewaters from industries, soda lakes, and alkaline springs have been reported in this review. Methodology: All the relevant papers published from 2013-2020 were looked over numerous sources like Google Scholar, Medline, PubMed, Research Gate, Science Direct, Scopus and Web of Science. Results: Most of the microbial life found in extreme alkaline habitats are found to form a variety of enzymes and an array of other substances of biotechnological interests. These enzymes, especially proteases, are exploited in industries globally because of their ability to withstand rigorous industrial reactions and conditions. Conclusion: Though a number of alkalophilic protease-producing bacteria have been isolated, still a large number of these micro-organisms are unidentified. The current demand for biotechnological products from them appeals to the need for isolation of unidentified bacteria.
- Published
- 2021
28. Assessing the Hepatotoxicity of Industrial Leachate; Histopathology and Heavy Metal Contents in Liver of Wistar Rats
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Nadeem Sheikh, Muddasir Hassan Abbasi, Muhammad Babar Khawar, and Rabia Mehmood
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Cadmium ,Toxicity ,medicine.medical_treatment ,lcsh:R ,Intraperitoneal injection ,Leachate ,Histopathology ,lcsh:Medicine ,chemistry.chemical_element ,Wastewater ,Animal science ,Heavy metals ,lcsh:Biology (General) ,chemistry ,Vacuolization ,medicine ,General Earth and Planetary Sciences ,lcsh:QH301-705.5 ,Saline ,Effluent ,General Environmental Science - Abstract
The process of paper production requires a huge quantity of water and energy and in turn contributes a number of effluents in the form of phenolics, toxic organic compounds and heavy metals in wastewater (leachate). The present investigation was aimed to assess the toxic effects of leachate on liver micro-architecture and heavy metal elements of the liver. Eighteen (18) healthy male Wistar rats (240 ± 10g) were selected and acclimatized prior to experimental treatment. These rats were randomly divided into three groups viz, Control group (received 4ml/ kg normal saline), Group 1 (4ml/ kg leachate) and Group 2 (4ml/ kg 1:10 diluted leachate). All the animals were dissected and liver tissues were collected and processed accordingly after 24 h of leachate treatment. High level of cadmium and chromium were found in Group 1 as compared to the control group upon liver metal contents analysis found out by flame atomic absorption spectrometer. A clear disruption of micro-architecture of the liver, congested sinusoids, damaged central vein, and perturbed morphology was observed in Group-1 as revealed by H & E staining. Moreover, loss of polarity, congestion, and disruption of hepatocytes and pronounced vacuolization in the cytoplasm was observed in Group 2 compared to control sections. On the basis of above findings, it can be concluded that paper industry leachate is highly toxic and its intraperitoneal injection results in hepatotoxicity that not only affects the hepatic micro-architecture but also results in perturbed liver metal contents. Therefore, proper treatment of such wastewater is required before its disposal.
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- 2018
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29. FORMULATION AND EVALUATION OF INDOMETHACIN LOADED NANOSPONGES FOR ORAL DELIVERY
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Komal Sarwar, Nasir Abbas, Muhammad Arshad, Rabia Mehmood, Pervaiz Akhtar Shah, Muhammad Irfan, and Amjad Hussain
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Pharmacology ,Chemistry ,Nanosponges ,Pharmaceutical Science ,Biomedical engineering - Published
- 2018
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30. Multifactorial role of long non-coding RNAs LncRNAs in hematopoiesis
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Muddasir Hassan Abbasi, Rabia Mehmood, Nadeem Sheikh, and Muhammad Babar Khawar
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0301 basic medicine ,General Immunology and Microbiology ,Cellular differentiation ,Cell Differentiation ,Computational biology ,Biology ,ENCODE ,Genome ,General Biochemistry, Genetics and Molecular Biology ,Hematopoiesis ,03 medical and health sciences ,Haematopoiesis ,030104 developmental biology ,Gene expression ,Protein biosynthesis ,Animals ,Humans ,RNA, Long Noncoding ,Human genome ,Gene - Abstract
Human genome project unveiled that only 1.5.-2.0.% of the genome is protein coding. ENCODE and related studies showed that most part of the genome transcribed into RNAs, and most of them do not code for a functional proteins, hence the name non-coding RNAs (ncRNAs). ncRNAs are small ncRNAs (less than 200 nucleotides) and long ncRNAs (longer than 200 nucleotides up to 10 kb). They act as a direct link between highly ordered chromosome structures, gene expression and serve as a bridge between genome and chromatin modification complexes as guides, scaffolds, and decoys. Highly regulated hematopoietic differentiation is required for formation of all types of blood cells. Among a variety of lncRNAs only few hematopoitic lncRNAs have been studied extensivelyand most of them are not functionally characterized. The role of these lncRNAs remains partially undetermined but their involvement in the regulation of various genes and protein synthesis has been proved even in hematopoiesis. So, the present review is a mere effort to highlight the role of lncRNAs involved in the development and regulation of hematopoiesis.
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- 2018
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31. Surveillance, Authoritarianism and ‘Imperial Effects’ in Pakistan
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Mahvish Ahmad and Rabia Mehmood
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business.industry ,Authoritarianism ,Military aid ,DS Asia ,United States National Security Agency ,Drone ,Urban Studies ,Politics ,Brexit ,Political economy ,Law ,Political science ,JZ International relations ,U Military Science ,business ,Safety Research ,Eurocentrism ,Expansive - Abstract
To speak of ‘Surveillance and the Global Turn to Authoritarianism’ presupposes a moment with little connection to that which has gone before, or places outside of North America and Europe. While Trump and Brexit inaugurate a consequential shift, even rupture, in the political terrain, we must not lose sight of places and peoples where American Wars – with European support – were overtly and covertly waged in the decades preceding this ‘global turn’, nor the fate of these places today. We argue that the sustained transfer of sophisticated surveillance technologies, as part and parcel of both direct military assault and more expansive support for security states, has had lasting imperial effects outside imperial centres that reverberate today. We take our point of departure in Pakistan – the site of hundreds of drone bombardments under Obama, one of the top recipients of US military aid, and the largest known recipient of funding from the National Security Agency (NSA) – to argue that ’global turns’ must not forget the rest of the world, and Surveillance Studies may have far to go before it fully addresses its Eurocentrism.
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- 2017
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32. Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development
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Saira Kainat Suqaina, Maryam Mukhtar, Naz Fatima, Sabeen Nazir, Andleeb Batool, Nadeem Sheikh, and Rabia Mehmood
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Article Subject ,Genotype ,lcsh:Medicine ,Arthritis ,Calcitriol receptor ,Polymorphism, Single Nucleotide ,General Biochemistry, Genetics and Molecular Biology ,Arthritis, Rheumatoid ,03 medical and health sciences ,0302 clinical medicine ,Calcitriol ,Internal medicine ,medicine ,Vitamin D and neurology ,Humans ,Genetic Predisposition to Disease ,Allele ,Vitamin D ,Genotyping ,Alleles ,030203 arthritis & rheumatology ,General Immunology and Microbiology ,business.industry ,lcsh:R ,Haplotype ,General Medicine ,Middle Aged ,medicine.disease ,030104 developmental biology ,Endocrinology ,Case-Control Studies ,Receptors, Calcitriol ,Female ,Gene polymorphism ,business ,Research Article - Abstract
Vitamin D is an anti-inflammatory molecule and has a role in prevention of arthritis development. Biologically active form 1, 25(OH)2D3 of vitamin D can only exert its action after binding its definite vitamin D receptor encoded by VDR gene. VDR gene polymorphism leads to dysfunctioning of 1, 25(OH)2D3 ultimately disease onset. The purpose of current study was to evaluate the effect of vitamin D level and VDR gene polymorphism on rheumatoid arthritis and osteoarthritis. Blood samples were collected from case and control after taking written consent. Serum was separated and vitamin D level as determined from each sample by ELISA. DNA was extracted from each blood sample and amplified by using gene specific primers. Genotyping was performed by Sangers sequencing and PCR-RFLP technique. It was found that vitamin D level was not significantly different among patients and controls. The rs10735810, rs1544410, rs7975232, and rs731236 were associated with the onset of arthritis at both allelic and genotypic level (p < 0.01). Nucleotide change on rs10735810 site leads to change of tryptophan with arginine. The frequencies of haplotype CGAT, CGGA, CGGT, CTAA, CTAT, TGAA, TGAT, TGGA, and TTGA were higher in patients and act as risk factors of RA onset, whereas haplotypes CGAT, CGAT, CGGT, CTGA, TGAT, TGGA, TTAA, and TTGA were associated with OA onset. In conclusion, serum vitamin D level may be normal among arthritis patients but polymorphism on VDR gene restricts vitamin D to perform its anti-inflammatory function by altering the 1, 25(OH)2 D3 binding sites.
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- 2018
33. MicroRNAs: Recent insights towards their role in male infertility and reproductive cancers
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Muhammad Babar Khawar, Rabia Mehmood, and Nabila Roohi
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0301 basic medicine ,Infertility ,Male ,Cell type ,Disease ,Review Article ,Bioinformatics ,Male infertility ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,medicine ,Biomarkers, Tumor ,Animals ,Humans ,Reproductive system ,Spermatogenesis ,noncoding RNAs ,Infertility, Male ,Regulation of gene expression ,lcsh:R5-920 ,business.industry ,Cancer ,Gene Expression Regulation, Developmental ,reproductive cancers ,General Medicine ,medicine.disease ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,miRNAs ,Genital Neoplasms, Male ,lcsh:Medicine (General) ,business - Abstract
Spermatogenesis is a tightly controlled, multi-step process in which mature spermatozoa are produced. Disruption of regulatory mechanisms in spermatogenesis can lead to male infertility, various diseases of male reproductive system, or even cancer. The spermatogenic impairment in infertile men can be associated with different etiologies, and the exact molecular mechanisms are yet to be determined. MicroRNAs (miRNAs) are a type of non-protein coding RNAs, about 22 nucleotides long, with an essential role in post-transcriptional regulation. miRNAs have been recognized as important regulators of various biological processes, including spermatogenesis. The aim of this review is to summarize the recent literature on the role of miRNAs in spermatogenesis, male infertility and reproductive cancers, and to evaluate their potential in diagnosis, prognosis and therapy of disease. Experimental evidence shows that aberrant expression of miRNAs affects spermatogenesis at multiple stages and in different cell types, most often resulting in infertility. In more severe cases, dysregulation of miRNAs leads to cancer. miRNAs have enormous potential to be used as diagnostic and prognostic markers as well as therapeutic targets in male infertility and reproductive system diseases. However, to exploit this potential fully, we need a better understanding of miRNA-mediated regulation of spermatogenesis, including the characterization of yet unidentified miRNAs and related regulatory mechanisms.
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- 2018
34. Head and Neck Cancer: Epidemiology and Role of MicroRNAs
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Muhammad Babar Khawar, Naz Fatima, Muddasir Hassan Abbasi, Rabia Mehmood, Saira Kainat Suqaina, and Nadeem Sheikh
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Head and neck cancer ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,microRNA ,Epidemiology ,medicine - Published
- 2017
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35. A novel single nucleotide polymorphism in exon 3 of MYOC enhances the risk of glaucoma
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Naz Fatima, Sabeen Nazir, Rabia Mehmood, Nadeem Sheikh, Maryam Mukhtar, Shaima Farooqi, and Maryam Shahnawaz
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Male ,0301 basic medicine ,Heredity ,Eye Diseases ,genetic structures ,lcsh:Medicine ,Glaucoma ,Artificial Gene Amplification and Extension ,Polymerase Chain Reaction ,Biochemistry ,Exon ,0302 clinical medicine ,Risk Factors ,Genotype ,Medicine and Health Sciences ,lcsh:Science ,Genetics ,Multidisciplinary ,Database and informatics methods ,Sequence analysis ,Exons ,Middle Aged ,Genetic Mapping ,Female ,Research Article ,Bioinformatics ,Motor Proteins ,Single-nucleotide polymorphism ,Biology ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,Molecular Genetics ,03 medical and health sciences ,Molecular Motors ,medicine ,Humans ,Allele ,Eye Proteins ,Molecular Biology Techniques ,Molecular Biology ,DNA sequence analysis ,Myocilin ,Glycoproteins ,lcsh:R ,Haplotype ,Case-control study ,Biology and Life Sciences ,Proteins ,Human Genetics ,Cell Biology ,medicine.disease ,eye diseases ,Cytoskeletal Proteins ,Ophthalmology ,030104 developmental biology ,Haplotypes ,Case-Control Studies ,Genetics of Disease ,030221 ophthalmology & optometry ,lcsh:Q - Abstract
Genetic polymorphismsof MYOCalter the myocilin protein,which leads to disruption of thenormal regulation of intraocular pressure (IOP) that ultimately causes glaucoma.Theaim of the present study was to identify the polymorphism in exon 3 of the MYOC gene of theglaucoma patients in Lahore, Pakistan. We conducted a case-control study with 100 patients and 100 controls subjects. We extracted DNA from blood samples,amplified the target DNA fragmentby PCR, and identifiedpolymorphisms through sequencing. We observed that the allelic and genotypic frequencies of rs74315341 and rs879255525 were associated with glaucoma in our patient population. The polymorphism atrs74315341 led to the substitutionof serine for arginine,whereas the polymorphism at rs879255525 led to the substitution ofasparagine for lysine. The haplotype TGAAGCCATTTC was associated with disease onset, whereas the haplotype GGAAGCCATTTC was protective against disease development. In conclusion, weidentified MYOC gene polymorphisms in susceptible regions that were associated withglaucoma onset among the Lahore patient population.This is the first report to identify a novel mutation in rs879255525 in exon 3 of the MYOC genethat is associated withglaucoma.
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- 2018
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36. Comparative Evaluation of Functional Properties of Some Commonly Used Cereal and Legume Flours and Their Blends
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Haq Nawaz, Rabia Mehmood, Hira Munir, Tanzila Rehman, and Muhammad Aslam Shad
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Gelation and Foaming properties ,Chemistry ,Cereal and legume flour ,Absorption and swelling capacities ,Swelling capacity ,Significant difference ,lcsh:S ,lcsh:TX341-641 ,lcsh:S1-972 ,Bulk density ,Comparative evaluation ,lcsh:Agriculture ,Agronomy ,Water holding capacity ,Functional properties of Blends ,lcsh:Agriculture (General) ,Protein solubility ,lcsh:Nutrition. Foods and food supply ,Legume - Abstract
Functional properties such as protein solubility, swelling capacity, water holding capacity, gelling ability, bulk density and foaming capacity of flours of some commonly used cereals and legume (wheat, refined wheat, maize and chickpea) and their blends were studied. Blends of flours were prepared by mixing equal proportions of selected floors. Statistically significant difference in studied functional properties except bulk density was observed among cereal flours and their blends. Chickpea flour was found to possess comparatively high water holding capacity, protein solubility index and swelling capacity. The functional properties of maize and wheat flours were found to be improved when blended with chickpea. Chickpea flour and its blends with cereal flours were found to possess good functional score and suggested as favorable candidates for use in the preparation of viscous foods and bakery products. The data provide guidelines regarding the improvement in functional properties of economically favorable cereal flours.
- Published
- 2015
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- View/download PDF
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