106 results on '"Radošević K"'
Search Results
2. Natural deep eutectic solvents improve the solubility of acetylcholinesterase reactivator RS194B
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Panić, M., Maček Hrvat, N., Štokić, M., Radojčić Redovniković, I., Kovarik, Z., and Radošević, K.
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- 2022
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Catalog
3. Ionic Liquids Development and Challenges in Industrial Application
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Cvjetko Bubalo, M., Radošević, K., Radojčić Redovniković, I., Halambek, J., Vorkapić-Furač, J., and Gaurina Srček, V.
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green processes ,Ionic liquids ,designer solvents ,Chemistry ,QD1-999 - Abstract
Establishment of novel, highly productive, and sustainable processes for the production of industrially important compounds is becoming a growing area of research. Due to non-volatility, inflammability, great thermal, chemical and electrochemical stability and also recyclability, ionic liquids are extensively studied as possible green replacement for widely used conventional molecular solvents. Due to the extremely large number of possible chemical structures of ionic liquids, the ability to design ionic liquids for specific applications makes them unique solvents in electrochemistry, analytical chemistry, organic synthesis and (bio)catalysis, separation processes, as well as functional fluids (lubricants, heat transfer fluids, corrosion inhibitors etc.). This paper presents a review of the scientific and technical literature related to ionic liquids, their basic properties, preparation and application, as well as the challenges of their application on an industrial scale. more...
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- 2014
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4. Assessment of Lymphokine-Activated Killer Activity Against Myeloid Leukemic Blasts and the Myeloid Cell Line K562 by Flow Cytometry
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Garritsen, H. S. P., Segers-Nolten, G. M. J., Radoševic, K., de Grooth, B. G., Kiehl, M., Wörmann, B., Hiddemann, W., Greve, J., Hiddemann, W., editor, Büchner, T., editor, Wörmann, B., editor, Plunkett, W., editor, Keating, M., editor, and Andreeff, M., editor more...
- Published
- 1992
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5. RATIONAL DESIGN OF NATURAL DEEP EUTECTIC SOLVENTS FOR CHIRAL DRUG PREPARATION
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Panić M., Radović M., Cvjetko Bubalo M., Radošević K., Gaurina Srček, V., and Radojčić Redovniković, I.
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Chiral drug ,COSMO-RS ,Natural deep eutectic solvents ,Rational design - Abstract
Designing new, environmentally-friendly, and tunable solvents have been dramatically expanding in popularity in order to overcome the flaws of organic solvents from technological, environmental and economic aspects. Natural deep eutectic solvents (NADES), as a new generation of novel alternative solvents, fully meet green and sustainable technologies principles. Though it is sometimes relatively easy to find an appropriate NADES for certain process, this is occasionally ungrateful procedure. Namely, sometimes is difficult to predict which mixtures and in which molar ratios will originate as a NADES and also which solvent properties crucial will be exerted. Therefore, the composition of NADES and their physical, thermal, chemical or biological properties should be characterized on case-by-case scenario. Abundance of possible NADES formulations, especially when possible addition of certain amount of water is taken into account, makes it impossible to prepare and characterize all of them, pointing out the need for development of predictive NADES structure-activity mathematical models. Approaches used so far for screening appropriate/ideal NADES (out of enormous pool of structural possibilities) have been governed by time consuming empirical methods, while systematic investigation in NADES structure-activity relationship, as a backbone for rational design of these solvents, is still lacking. Current literature suggests COSMO-RS, software that can predict thermodynamic properties and phase equilibrium which would help in designing the best possible solvent for a certain application. COSMO-RS can design new solvent structures with tailored properties and thus the trial-and-error method of NADES preparation can be avoided. The aim of this work is to design rationally NADES for the preparation of therapeutic deep eutectic solvents and biocatalytic preparation of chiral drug by combining experimental and modelling tools. more...
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- 2021
6. Sustainable waste management from wine production
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Panić, M., Gunjević, V, Radović, M, Radošević, K., Cvjetko Bubalo, M., Ćurko, N., Kovačević Ganić, K., and Radojčić Redovniković, I.
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COSMO-RS ,green extraction ,natural deep eutectic solvents ,polyphenols - Abstract
Food processing generates a substantial volume of solid organic by-products. Only small amounts of these by-products are up-graded or recycled and used usually for composting or even discarded in open areas potentially causing environmental problems. Wine production by products, such as grape pomace, are the most abundant worldwide and have been lately often evaluated as a source of biological active compounds due to high content of polyphenolics. In the last few years, the extraction and identification of phenolic compounds from natural sources has become a major area of scientific research. Due to their complex structure, there is no universal extraction method suitable for extraction of all plant phenolic, whereby conventional extraction techniques are usually associated with high organic solvent consumption and long extraction times. One of the organic solvents replacement could be natural deep eutectic solvents (NADES) since NADES can dissolve both polar and non-polar compounds. They may be used for very efficient extraction of natural products from plants, such as polyphenols. Since NADES has high safety profile it is expected that extract obtained by NADES may be directly used in food, pharmaceutical, cosmetical and agrochemical products without the need for expensive downstream purification steps. NADES is designed solvents, so to save time and money, it should be designed rationally for polyphenolics extraction. Currently, COSMO-RS can design new solvent structures with tailored properties and thus the trial-and-error method of NADES preparation can be avoided Therefore, the aim of this stufy is to design efficient NADES for extraction of polyphenols from wine production by-products. more...
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- 2021
7. DESIGNING A BIOCATALYTIC PROCESS INVOLVING DEEP EUTECTIC SOLVENTS: LIPASE-CATALYSED SYNTHESIS OF (R)-1- PHENYLETHANOL AS A CASE STUDY
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Radović M., Panić M., Jurinjak Tušek, A., Cvjetko Bubalo M., Radošević K., and Radojčić Redovniković I.
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Biocatalysis ,Green chemistry ,Natural deep eutectic solvents - Abstract
The synergistic use of deep eutectic solvents (DES) and biocatalyst fits logically to the efficient and sustainable production of various commercially interesting products. Namely, biocatalysis ensures catalyzing otherwise difficult transformations in high regio-, chemo- and enantioselective manner at mild and economic conditions, whereby DES can serve as strong green support for modulating/directing reaction route for obtaining the desired product. As the number of structural combinations encompassed by DES is tremendous, it is possible to design an optimal DES for each specific enzymatic reaction system. This important feature of DES allows to (i) enhance enzyme stereoselectivity, activity and stability, as well as reaction rate through enhanced substrate solubility ; (ii) enhance reaction yield (iii) enhance/modify biocatalyst stereo preference ; (iv) contribute to overall greenness of the process (including recycle and reuse). The use of DES for enzyme-catalysed reaction could be considered as “back to the roots”. Namely, when the compounds that constitute the DES are primary metabolites, namely, amino acids, organic acids, sugars or choline derivatives, they can provide cytoplasm-like natural environment for enzymes, meaning that enzymes can transform unnatural substrates in natural environment. When designing efficient biocatalytic process involving DES, independently of reaction type and enzyme used, the following steps should be included: (i) preparation and characterization of DES (ii) screening of DES for optimal enzyme performance (iii) optimization of the biocatalytic process, and (iv) downstream process design with possible scale-up. In this work green lipase-catalysed process for obtaining commercially interesting secondary alcohol (R)-1- phenylethanol of high optical purity in DES as medium was development. Several cholinium chloride-based DES were prepared and screened for optimal enzyme performance (enzyme activity, stability and enantioselectivity), followed by optimization of biocatalytic process (substrate and enyzme loading, water content in DES, reaction temperature). Recovery of the products/DES and DES recycling was successfully performed. After laboratory scale, production of (R)-1- phenylethanol was also effectively performed on a preparative scale (half liter batch reactor). The results of this study confirmed the great potential of DES in biocatalytic medium in terms of improved lipase stability and process sustainability. more...
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- 2021
8. POTENTIAL APPLICATION OF NATURALDEEP EUTECTIC SOLVENTS IN FOOD INDUSTRY
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Panić, M., Radović, M., Cvjetko Bubalo, M., Radošević, K., and Radojčić Redovniković, I.
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Food fortification ,Green extraction ,Natural deep eutectic solvents ,Polyphenolic compounds - Abstract
Many industrial processes use large quantities of volatile, flammable and toxic organic solvents based on unsustainable resources such as oil, resulting in significant environmental and economic impacts. Over the past decade, natural deep eutectic solvents (NADES) have become promising alternatives to traditional organic solvents from both environmental and technological perspectives. The choice of a solvent in certain process not only depends on its chemical, and physical properties, but also on its environmental impact (e.g. ecotoxicity and biodegradability), sustainability (possibility of recycle and reuse) and process safety (e.g. flammability and volatility). Therefore, interest for potential application of NADES as solvents and/or supporting medium in different processes has increased significantly, due to their unique physical and chemical properties, and low environmental impact. One of the major attractions of making NADES an alternative to conventional organic solvents lies in the fact that the number of structural combinations encompassed by these solvents is tremendous, thus it is possible to design an optimal one for each specific application. Since the number of possible chemical structures of these solvents is vast NADES are very interesting for use in food industry. Potential use of NADES for extraction of biological active compounds, preparation of ready- touse extracts and use of NADES-based extracts for fortification in food industry will be considered herein. more...
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- 2021
9. EVALUATION OF BIOLOGICAL ACTIVITY OF PLANT-NADES- EXTRACTS AS READY-TO-USE EXTRACT FOR FOOD AND PHARMACEUTICAL APPLICATION
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Panić M., Radović M., Cvjetko Bubalo M., Radojčić Redovniković, I., Gaurina Srček V., and Radošević, K. K.
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Biological activitiy ,In vitro testing ,Natural deep eutectic solvents ,Plant extracts - Abstract
Since NADES consist of simple, inexpensive and naturally occurring compounds with an implied high safety profile, it is assumed that extracts obtained by NADES may be directly used in products for human consumption without the need for expensive downstream purification steps. Considering the potential applications of NADES and NADES-based extracts, it is necessary to further evaluate their biological activity. Human cell lines are already widely used in testing biological activity of compounds present in plants and plant’s products. The application of in vitro cell cultures for this purpose is based on the fact that interaction of the test substance and biological systems occurs preferentially at the cellular level, and has an impact on the whole organism. With such approach a large number of samples, including crude extracts from screening phase, as well as pure fractions obtained during the isolation procedure, could be evaluated. Beyond that, the application of in vitro cell cultures is a good choice for preliminary screen of biological activities, which enables faster and more cost-effective studies compared to in vivo studies. The findings obtained by in vitro tests are valuable and can serve as guidelines for further required in vivo and epidemiological studies of biological activity of plants, its compounds, and products. Based on aforementioned, potential biological activity of plant polyphenolic extracts was evaluated by testing antiproliferative and antioxidant activity. In vitro cytotoxicity of prepared extracts was assessed by antiproliferation assay on two tumour cell lines, whereas for investigation of type of cell death and cell cycle arrest a flow cytometric analysis was applied. Antioxidant activity and possibility of extracts to protect cells from oxidative stress was assessed by ORAC method as well as cell-based methods. more...
- Published
- 2021
10. Aujeszky’s disease virus production in disposable bioreactor
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Slivac, I., Srček, V. Gaurina, RadoŠević, K., Kmetič, I., and Kniewald, Z.
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- 2006
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11. “Natural resources, green technology and sustainable development/4-GREEN2022”.
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Radošević, K. and Jakovljević, T.
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- 2022
12. Niskotemperaturna eutektička otapala – racionalnim dizajnom do zelenog otapala budućnosti.
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Radović, M., Panić, M., Radošević, K., Cvjetko Bubalo, M., and Radojčić Redovniković, I.
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SUSTAINABLE chemistry ,FOOD biotechnology ,ORGANIC synthesis ,ECONOMIC demand ,ELECTROCHEMISTRY ,BIOACCUMULATION - Abstract
Copyright of Kemija u Industriji is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2021
- Full Text
- View/download PDF
13. Biotransformation of methoxyflavones in entomopathogenic fungi strains
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Łużny M, Radošević K, Kozłowska E, Kostrzewa- Susłow E, Srček VG, Janeczko T.
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fungi ,food and beverages ,biotransformations, entomopathogenic fungi strains, methoxyflavones - Abstract
Flavonoids occur in many forms in nature (including hydroxy-, methoxy- and glycoside- derivatives). However, glycosidic derivatives are the most soluble and assimilable. Acquiring more of these compounds from plants requires the use of a large amount of solvents, while their chemical synthesis is very complicated and unprofitable. Biotransformations using entomopathogenic fungi strains allow to obtaining glycoside derivatives with high yield and keeping the principles of "green chemistry". Flavonoids are common in the plant world, where they fulfill many very important functions (including: protective against UV, function as growth regulators, fungicides, or responsible for the plant coloration). They are present in our diet and in the human body they exhibit e.g. antioxidant, antimicrobial or anticancer properties. The ability to perform glycosylation of flavonoids by entomopathogenic fungi is already well known. This process is used to increase the stability and bioavailability of flavonoid compounds. Entomopathogenic fungi strains used in the experiment come from Lower Silesia and belong to four species: Beauveria bassiana, Beauveria caledonica, Isaria farinosa and Isaria fumosorosea. more...
- Published
- 2019
14. A model system for optimising the selection of membrane antigen-specific human antibodies on intact cells using phage antibody display technology
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Hegmans, J.P.J.J, Radosevic, K, Voerman, J.S.A, Burgers, J.A, Hoogsteden, H.C, and Prins, J.-B
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- 2002
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15. Physicochemical Properties, Cytotoxicity, and Antioxidative Activity of Natural Deep Eutectic Solvents Containing Organic Acid.
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Mitar, A., Panić, M., Kardum, J. Prlić, Halambek, J., Sander, A., Kučan, K. Zagajski, Redovniković, I. Radojčić, and Radošević, K.
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EUTECTICS ,ORGANIC acids ,CORROSION & anti-corrosives ,CELL-mediated cytotoxicity ,REACTIVE oxygen species - Abstract
Natural deep eutectic solvents (NADES) may be considered 'designer solvents' due to their numerous structural variations and the possibility of tailoring their physicochemical properties. Prior to their industrial application, characterization of NADES is essential, including determination of their physicochemical properties, cytotoxicity, and antioxidative activity. The most important physicochemical properties of eight prepared NADES (choline chloride:malic acid, proline:malic acid, choline chloride:proline:malic acid, betaine:malic acid, malic acid:glucose, malic acid:glucose:glycerol, choline chloride: citric acid, and betaine:citric acid) were measured as functions of temperature and water content. In general, the structure of prepared NADES greatly influences their physical properties, which could be successfully modified and adjusted by addition of water. All tested NADES were absolutely benign and noncorrosive for investigated steel X6CrNiTi18-10. Furthermore, cytotoxicity of prepared solvents was assessed toward three human cell lines (HEK-293T, HeLa, and MCF-7 cells), and antioxidative activity was measured by the Oxygen Radical Absorbance Capacity (ORAC) method. With regard to cell viability, all tested NADES containing carboxylic acid could be classified as practically harmless and considered environmentally safe. The ORAC values indicated that the tested NADES displayed antioxidative activity. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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16. H5N1 Protection by Seasonal Influenza Vaccine in Homologous and Heterologous Prime/Boost Vaccination
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Vreugdenhil J, Radošević K, Goudsmit J, Damman-Riahi S, Roozendaal R, Roos A, Koldijk M, Dekking L, and Vaneman J
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biology ,Immunogenicity ,Immunology ,virus diseases ,Priming (immunology) ,Heterologous ,medicine.disease_cause ,Virology ,Influenza A virus subtype H5N1 ,Virus ,Vaccination ,Drug Discovery ,Homologous chromosome ,medicine ,biology.protein ,Immunology and Allergy ,Antibody - Abstract
Background A number of different approaches aimed at broadening the cross-protective ability of seasonal influenza vaccines are being explored today. Priming a seasonal vaccine with three administrations of DNA encoding H1 HA corresponding to the HA in the seasonal vaccine has been shown to confer protection against heterologous H1N1 influenza. Here we evaluated the heterosubtypic protection induced by a seasonal influenza vaccine when primed with H1 HA DNA and, in parallel, when given as a homologous prime/boost regimen. Methods Balb/c mice were immunized three times with vaccine homologous H1 HA DNA prior to a boost with seasonal influenza vaccine (season 2009/2010; Northern Hemisphere), or immunized three times with the seasonal influenza vaccine. To assess cross-protection, mice were subsequently challenged with either heterologous H1N1 or heterosubtypic H5N1 influenza virus. Results The level of heterologous H1N1 protection elicited by the seasonal influenza vaccine was enhanced by priming with H1 HA DNA. In contrast, priming with H1 HA DNA did not enhance the level of heterosubtypic H5N1 protection. The heterologous prime boost regimen showed to be less efficient than multiple immunizations with seasonal vaccine in conferring protection against H5N1. Neither the DNA-priming vaccination regimen, nor the homologous prime/boost regimen induced detectable H5N1 cross-reactive anti-HA or anti-NA antibodies. Homologous prime /boost vaccination did induce higher levels of anti-NP antibodies. Conclusion Here we demonstrate that priming a seasonal influenza vaccine with vaccine homologous H1 HA encoding DNA enhances the level of heterologous H1N1 but not heterosubtypic protection induced by the vaccine alone. Homologous prime/boost vaccination resulted in higher levels of heterosubtypic protection. Of the immunogenicity parameters tested for both heterologous and homologous prime/boost regimens only anti-NP responses follow the same pattern as heterosubtypic protection. more...
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- 2015
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17. Detekcija rane apoptoze u kulturi neonatalnih kardiomiocita
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Kniewald, H, Polančec, D, Malčić, I, Radošević, K, Gaurina Srček, V., Mustapić, Ž, Dorner, S., and Kniewald, Z
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apoptoza ,kultura neonatalnih miocita - Abstract
JCl bojanje i protočna citometrija su metode koje su primjenjive za detekciju ranih apoptotičkih promjena nakon indukcije apoptoze neonatalnih kardiomicita adrenergičkim putem
- Published
- 2006
18. Human CD46-transgenic mice in studies involving replication-incompetent adenoviral type 35 vectors
- Author
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Verhaagh, S., Jong, E. de, Goudsmit, J., Lecollinet, S., Gillissen, G., Vries, M. de, Leuven, K. van, Que, I., Ouwehand, K., Mintardjo, R., Weverling, G.J., Radošević, K., Richardson, J., Eloit, M., Lowik, C., Quax, P., Havenga, M., Verhaagh, S., Jong, E. de, Goudsmit, J., Lecollinet, S., Gillissen, G., Vries, M. de, Leuven, K. van, Que, I., Ouwehand, K., Mintardjo, R., Weverling, G.J., Radošević, K., Richardson, J., Eloit, M., Lowik, C., Quax, P., and Havenga, M. more...
- Abstract
Wild-type strains of mice do not express CD46, a high-affinity receptor for human group B adenoviruses including type 35. Therefore, studies performed to date in mice using replication-incompetent Ad35 (rAd35) vaccine carriers may underestimate potency or result in altered vector distribution. Here, it is reported that CD46 transgenic mice (MYII-strain) express CD46 in all major organs and that it functions as a receptor for rAd35 vectors. Similar to monkeys and humans, MYII mice highly express CD46 in their lungs and kidneys and demonstrate low expression in muscle. Upon intravenous administration, rAd35 vector genomes as well as expression are detected in lungs of MYII mice, in contrast to wild-type littermates. Expression was predominantly detected in lung epithelial cells. Upon intramuscular administration, the initial level of luciferase expression is higher in MYII mice as compared with wild-type littermates, in spite of the fact that CD46 expression is low in muscle of MYII mice. The higher level of expression in muscle of MYII mice results in prolonged gene expression as assessed by CCD camera imaging for luciferase activity. Finally, a significant dose-sparing effect in MYII mice as compared with wild-type littermates on anti-SIVgag CD8+ T-cell induction following intramuscular vaccination with an rA35.SIVgag vaccine was observed. This dose-sparing effect was also observed when reinfusing dendritic cells derived from MYII mice after exposure to rAd35.SIVgag vaccine as compared with rAd35.SIVgag exposed dendritic cells from wild-type littermates. It was concluded that MYII mice represent an interesting preclinical model to evaluate potency and safety of rAd35 vectors. © 2006 SGM. Chemicals / CAS: Antigens, CD46 more...
- Published
- 2006
19. Growth and metabolic characteristics of CCO cells in static and dynamic cultivation systems
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Slivac, I., primary, Radošević, K., additional, Srček, V. Gaurina, additional, Fumić, K., additional, and Kniewald, Z., additional
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- 2008
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20. Immunogenicity and Protection of a Recombinant Human Adenovirus Serotype 35-Based Malaria Vaccine against Plasmodium yoelii in Mice
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Ophorst, O. J. A. E., primary, Radošević, K., additional, Havenga, M. J. E., additional, Pau, M. G., additional, Holterman, L., additional, Berkhout, B., additional, Goudsmit, J., additional, and Tsuji, M., additional more...
- Published
- 2006
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21. Ionske kapljevine -- razvoj i izazovi industrijske primjene.
- Author
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Bubalo, M. Cvjetko, Radošević, K., Redovniković, I. Radojčić, Halambek, J., Vorkapić-Furač, J., and Srček, V. Gaurina
- Subjects
IONIC liquids ,ELECTROCHEMICAL research ,ANALYTICAL chemistry research ,ORGANIC synthesis research ,BIOCATALYSIS ,HEAT transfer fluids - Abstract
Copyright of Kemija u Industriji is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2014
- Full Text
- View/download PDF
22. Kulture životinjskih stanica.
- Author
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Radošević, K.
- Published
- 2020
23. The impact of proline isomerization on antigen binding and the analytical profile of a trispecific anti-HIV antibody
- Author
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Alessandro Masiero, Lechat Nelly, Gentric Marianne, Sourrouille Christophe, Laville Florian, Crépin Ronan, Borel Claire, Ziegler Cornelia, Bisch Grégoire, Leclerc Eric, Laurent Ludovic, Brault Dominique, Alexandre Sylvie, Gagnaire Marie, Duffieux Francis, Soubrier Fabienne, Capdevila Cécile, Arnould Isabelle, Dumas Jacques, Dabin Jérôme, Genet Bruno, Radošević Katarina, Menet Jean-Michel, and Prades Catherine more...
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Proline isomerization ,antibody conformers ,chemical equilibrium ,developability ,Therapeutics. Pharmacology ,RM1-950 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Proline cis-trans conformational isomerization is a mechanism that affects different types of protein functions and behaviors. Using analytical characterization, structural analysis, and molecular dynamics simulations, we studied the causes of an aberrant two-peak size-exclusion chromatography profile observed for a trispecific anti-HIV antibody. We found that proline isomerization in the tyrosine-proline-proline (YPP) motif in the heavy chain complementarity-determining region (CDR)3 domain of one of the antibody arms (10e8v4) was a component of this profile. The pH effect on the conformational equilibrium that led to these two populations was presumably caused by a histidine residue (H147) in the light chain that is in direct contact with the YPP motif. Finally, we demonstrated that, due to chemical equilibrium between the cis and trans proline conformers, the antigen-binding potency of the trispecific anti-HIV antibody was not significantly affected in spite of a potential structural clash of 10e8v4 YPtransPtrans conformers with the membrane-proximal ectodomain region epitope in the GP41 antigen. Altogether, these results reveal at mechanistic and molecular levels the effect of proline isomerization in the CDR on the antibody binding and analytical profiles, and support further development of the trispecific anti-HIV antibody. more...
- Published
- 2020
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24. Immunogenicity and Protection of a Recombinant Human Adenovirus Serotype 35-Based Malaria Vaccine against Plasmodium yoeliiin Mice
- Author
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Ophorst, O. J. A. E., Radošević, K., Havenga, M. J. E., Pau, M. G., Holterman, L., Berkhout, B., Goudsmit, J., and Tsuji, M.
- Abstract
ABSTRACTGiven the promise of recombinant adenovirus type 5 (rAd5) as a malaria vaccine carrier in preclinical models, we evaluated the potency of rAd35 coding for Plasmodium yoeliicircumsporozoite protein (rAd35PyCS). We chose rAd35 since a survey with serum samples from African subjects demonstrated that human Ad35 has a much lower seroprevalence of 20% and a much lower geometric mean neutralizing antibody titer (GMT) of 48 compared to Ad5 (seroprevalence, 85%; GMT, 1,261) in countries with a high malaria incidence. We also demonstrated that immunization with rAd35PyCS induced a dose-dependent and potent, CS-specific CD8+cellular and humoral immune response and conferred significant inhibition (92 to 94%) of liver infection upon high-dose sporozoite challenge. Furthermore, we showed that in mice carrying neutralizing antibody activity against Ad5, mimicking a human situation, CS-specific T- and B-cell responses were significantly dampened after rAd5PyCS vaccination, resulting in loss of inhibition of liver infection upon sporozoite challenge. In contrast, rAd35 vaccine was as potent in naive mice as in Ad5-preimmunized mice. Finally, we showed that heterologous rAd35-rAd5 prime-boost regimens were more potent than rAd35-rAd35 because of induction of anti-Ad35 antibodies after rAd35 priming. The latter data provide a further rationale for developing rAd prime-boost regimens but indicate that priming and boosting Ad vectors must be immunologically distinct and also should be distinct from Ad5. Collectively, the data presented warrant further development of rAd35-based vaccines against human malaria. more...
- Published
- 2006
- Full Text
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25. Cytotoxic and apoptotic effects of 17α-ethynylestradiol and diethylstilbestrol on CHO-K1 cells
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Radošević, K., Novak, R., Igor Slivac, Mihajlović, M., Dumić, J., Kniewald, Z., and Srček, V. G.
- Subjects
lcsh:Food processing and manufacture ,17α-ethynylestradiol ,lcsh:TP368-456 ,apoptosis ,CHO-K1 cells ,cytotoxicity ,diethylstilbestrol ,necrosis ,lcsh:Biotechnology ,lcsh:TP248.13-248.65 - Abstract
There is considerable concern about the substances present in the environment and their potential to interfere with the endocrine system of vertebrates. Among these, the so-called endocrine-disrupting compounds, which can modulate or disrupt developmental and reproductive processes, substances with estrogenic activity have attracted most attention. Concerns about the presence of these compounds in the environment have led to the development of screening and testing assays that are able to detect such substances and evaluate their potential to induce adverse effects. In vitro systems such as mammalian and fish cell lines have become of growing importance in toxicity testing of such compounds. The cytotoxic and apoptotic effects induced by 17α-ethynylestradiol and diethylstilbestrol were studied on CHO-K1 cell line. Trypan blue exclusion method was used to determine the cell viability. Cytotoxicity of 17α-ethynylestradiol (0.34–34 μM) and diethylstilbestrol (0.37–37 μM) was found to be concentration-dependent with IC50 values of 12.8 and 10.4 μM after 72 h of exposure, respectively. In treated CHO-K1 culture cell death was assessed by determining morphological changes by haematoxilyn and eosin staining, nuclear morphology by fluorescein diacetate/propidium iodide staining and fluorescence microscopy, DNA fragmentation by TUNEL method and translocation of phosphatidyl serine by flow cytometry. The obtained results showed that 17α-ethynylestradiol induced apoptosis, while diethylstilbestrol induced necrosis in the treated CHO-K1 cells. more...
26. Cellulose acetate- and waste paper- based bionanocomposites: Optical and thermal properties
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Španić, Nikola, Plazonić, Ivana, Podvorec, Tomislav, Radojčić Redovniković, I., Jakovljević, T., Stojaković, R., Erdec, D., Panić, M., Damjanović, A., Radošević, K., and Cvjetko Bubalo, M.
- Subjects
bio- nano composites ,cellulose acetate ,filler ,properties ,waste paper - Abstract
Recycling of waste materials and production of new products with more added values are defined by the principles of the circular economy. Given that the circular economy imply also the continual use of resources and raw materials, this paper deals with the possibility of waste paper use as nano sized filler for cellulose acetete based bio composites. Potential use of multiple times recycled, waste packaging (Kraft) paper and laboratory filter paper as starting materials for nano sized cellulose based filler preparation was determined. As for cellulose acetate polymeric matrix synthesis, Black alder (Alnus glutinosa (L.) Gaertn.) wood and commercial microcrystalline cellulose (Avicel) were used. The optical (UV spectroscopy) and thermal (DSC) properties of prepared bio-nano composites were determined, with supplementary FT-IR analysis of synthesised cellulose acetates. The test results indicated the exceptional importance of the purity of the cellulose base both for the synthesis of nano particles and for the synthesis of the polymeric base of bio- nano composites. more...
- Published
- 2022
27. Testing of lindane cytotoxicity on Chinese Hamster Ovary (CHO-K1) cell line
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Kmetič, I., Gaurina-Srček, V., Radošević, K., and Kniewald, J.
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- 2006
- Full Text
- View/download PDF
28. Dendritic cells from diabetes-prone NOD mice induce a prolonged proliferative response of memory-like T cells
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Radoševic, K., Drexhage, H.A., van Ewijk, W., and Leenen, P.J.M.
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- 1997
- Full Text
- View/download PDF
29. KINETIC ANALYSIS OF FURFURAL INHIBITION ON POTENTIAL ETHANOL PRODUCING WILD STRAIN OF Saccharomyces cerevisiae
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Novak, Mario, Trontel, Antonija, Marđetko, Nenad, Mušak, Lucija, Šantek, Božidar, Horvat, Predrag, Radojčić Redovniković I., Jakovljević T., Petravić Tominac V., Panić M., Stojaković R., Erdec D., Radošević K., Gaurina Srček V., and Cvjetko Bubalo M. more...
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integumentary system ,furfural, yeast S. cerevisiae, ethanol production, kinetic analysis - Abstract
materials for different biotechnological processes [1, 2]. Pre-treatment of such materials is necessary due to their complex structure. With desirable products formed during pretreatment as glucose and xylose for example [1] a variety of undesirable by products are formed as organic acids and aromatic compounds (eg. furfural, formic acid, hidroxymetil furfural, levulinic acid, acetic acid)[3]. Those compounds have inhibitory effect on selected microorganism and on bioprocess efficiency [1, 4]. Origin of inhibitors can be categorized in three notable groups. First group are inhibitors present in lignocellulosic structure. Compounds as aldehydes and phenols are created during depolymerization of lignin. Acids as acetic, formic, galacturonic are formed during degradation of hemicellulose [4]. Second group of inhibitors are compounds that are formed during degradation of lignocellulosic materials. In the structure of lignin, generally they are present as non-inhibitors (pentose and hexose sugars). Under high temperature and acidic conditions, pentose sugars are degraded to furfural (furan 2- carbaldehyde) and hexose sugars to 5- hydroxymethyl furfural. Also 5- hydroxymethyl furfural can be degraded to levulinic acid and formic acid [3, 4, 5]. Third group of inhibitors are compounds present on lignocellulosic materials and are not integral part of materials itself. Most common are nitrogen and sulphur chemical compounds, heavy metals and different pesticides or other chemicals used in agriculture [3, 4, 5]. Furfural and hydroxymethyl furfural are two most important inhibitors that are created during lignocellulosic pre-treatment. Influence of furans (furfural and hydroximethylfurfural) on the biomass growth and activity can be used as an inhibition indicator for other compounds present in hydrolysates too [6, 7]. Set of experiments were carried out to investigate the inhibitory effect of furfural on the growth and ethanol production with wild strain Saccharomyces cerevisiae. Selected microorganism is potential ethanol producer from hydrolysates obtained with weak acid pre- treatment of lignocellulosic materials as wheat straw. more...
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- 2018
30. EFFECT OF FURFURAL ON GROWTH AND ACTIVITY OF LACTIC ACID BACTERIA
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Trontel, A., Novak, M., Marđetko, N., Kostić, A., Šantek, B., Radojčić Redovniković I., Jakovljević T., Petravić Tominac V., Panić M., Stojaković R., Erdec D., Radošević K., Gaurina Srček V., and Cvjetko Bubalo M. more...
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food and beverages ,influence of furfural, lactic acid bacteria, lactic acid production - Abstract
Lactic acid is mainly produced by lactic acid bacteria (LAB ; Lactobacillus sp., Streptococcus sp., Pediococcus sp.) and molds (e. g. Rhizopus oryzae, Rhizopus arrhizus)[1]. This bulk chemical can be produced from first and second generation raw materials [2]. First generation raw materials are feedstocks that contain sugar and starch and are widely used in industrial-scale lactic acid production. Second generation raw materials are ligncellulosic raw materials that have to be pretreated prior to fermentation [3]. During pretreatment variety of toxic compounds like furfural, hydroxymethylfurfural, levulinic and acetic acid and different lignin derivatives are beeing formed [4]. Obtained hydrolysates are usually treated by different expensive and long-lasting methods that can be used for detoxification procedures. Therefore, tolerance of working microorganism to above mentioned toxic compounds could be an asset in lactic acid production process [5]. Furans can be used as good predictor of the inhibition effect of other toxic substances present in lignocellulosic hydrolysate [2] and furfural was identified as main inhibiting compound obtained after dilute acid pretratment of lignocellulose [6]. Since there is limited amount of data available on toxic effect of inhibitors formed during pretreatment of lignocellulose [6], in this work the influence of different concentrations of furfural (from 100 to 5000 mg L-1) on growth and activity of lactic acid bacterium Lactobacillus amylovorus DSM 20531T in De Man-Rogosa-Sharpe (MRS) medium was investigated. more...
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- 2018
31. METHYLEN BLUE ADSORBENT BY AMORPHA FRUTICOSA L. ACTIVATED CARBON - PRELIMINARY STUDY
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Cindrić, Ines, Halambek, Jasna, Ožura, Marko, Radojčić Redovniković, I., Jakovljević, T., Petravić Tominac, V., anić, M., Stojaković, R., Erdec, D., Radošević, K., Gaurina Srček, V., and Cvjetko Bubalo, M. more...
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Adsorption, Amorpha fruticosa L., methylene blue - Abstract
In this study, activated carbon derived from Amorpha fruticosa L. was evaluated as a potential adsorbent for removal of methylene blue dyes (C.I. 52015). The adsorption process with respect to operating parameters (contact time, pH, adsorbent dose, temperature, concentration) was investigated to evaluate the adsorption characteristics. Research of equilibrium, kinetics and thermodynamic studies was performed. The results indicated that the maximum amount of adsorbed dye (99, 78%) was reached at pH 5, 5 and at 20 min. This study suggests a potential use of Amorpha fruticosa L. for the removal of cationic dye from aquatic media. more...
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- 2018
32. The impact of essential oils (laurel, basil and dill) on corrosion behaviour of Al-Mg alloy in model solutions
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Halambek, Jasna, Kralj, Elizabeta, Cindrić, Ines, Radojčić Redovniković, I., Jakovljević, T., Petravić Tominac, V., Panić, M., Stojaković, R., Erdec, D., Radošević, K., Gaurina Srček, V., and Cvjetko Bubalo, M. more...
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Al-Mg alloy, corrosion, essential oil, inhibition, model solution - Abstract
The essential oils have been in use for many years in industries, especially in the pharmaceutical, cosmetic and sanitary industry. Recently, many essential oils are used in culinary and food industry as spices and flavouring agents. Aluminium alloys with magnesium are one of the most important construction materials, especially in shipbuilding, but also for use in the chemical and food industry. Despite the good properties of Al-Mg alloys, they are not perfect materials for engineering applications in all environments, since they suffer from corrosion caused by chemical interactions with their surroundings. For the selection of aluminium and its alloys for the needs of the food industry, their good corrosion resistance to most food products is particularly important. High corrosively environment for aluminium and aluminium alloys in food industry are foodstuffs with pH 3 – 5, such as fruit juices, canned fruits, sauces as well as vegetables and fish pickled in brines with 1 –3 % salt. For this reason, NaCl and acetic acid needs to be taken into corrosion consideration, due to its wide usage in food industry. This study was carried out on AA5754 alloy (aluminium alloy with 3% of magnesium) using gravimetric and potentiodynamic polarization methods, while the alloy surfaces were examined by scanning electron microscopy (SEM). Corrosion study of AA5754 alloy in 3 % NaCl, 1 % CH3COOH and their mixture with addition of 20 ppm laurel, basil and dill essential oils as three very commonly used herbs to improve food flavorings, was conducted in temperature range 25°C to 80°C. Results show that the best inhibitory action on Al-Mg alloy corrosion have basil oil at all investigated temperatures, especially in mixture of 3% NaCl and 1% CH3COOH solutions. more...
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- 2018
33. Lactic acid production from wheat straw cellulose obtained after alkali pre-treatment in high-pressure reactor by Lactobacillus amylovorus DSM 20531T
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Trontel, Antonija, Vidović, Petra, Novak, Mario, Marđetko, Nenad, Šantek, Božidar, Radojčić Redovniković I., Jakovljević T., Petravić Tominac V., Panić M., Stojaković R., Erdec D., Radošević K., Gaurina Srček V., and Cvjetko Bubalo M. more...
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alkali pre-treatment in high-pressure reactor, enzymatic hydrolysis and fermentation, lactic acid, Lactobacillus amylovorus, wheat straw ,food and beverages - Abstract
Sugar and starch containing raw materials are widely used as food and feed thus, second generation raw materials like waste lignocellulosic biomass present sustainable alternative for production of different biochemicals, e.g. lactic acid. In this work, alkali pre-treatment of wheat straw with 2 % w/w sodium hydroxide solution in high-pressure reactor at different temperatures (from 120°C to 210°C) and residence times (from 1 to 20 minutes) was performed. After alkali pre-treatment of wheat straw, solid and liquid phase was obtained and composition of the two phases was determined. Solid phase (wheat straw cellulose) with high glucan content was used to study the fermentation capabilities of selected strain in two bioprocess types: Separate Hydrolysis and Fermentation (SHF) and Simultaneous Cellulose Hydrolysis and Fermentation (SCHF). Cellulose hydrolysis was performed with commercially obtained cellulases and β-glucosidases isolated from mold Aspergillus niger. In SHF bioprocess, after enzymatic hydrolysis of wheat straw cellulose, obtained hydrolysates were used for lactic acid production by lactic acid bacterium Lactobacillus amylovorus DSM 20531T. While, in SCHF bioprocess, enzymatic hydrolysis of wheat straw cellulose to simpler carbohydrates and fermentation by L. amylovorus DSM 20531T was performed simultaneously. Process efficiency parameters, like final lactic acid concentration, lactic acid yield and bioprocess productivity, in SCHF bioprocess were approximately 60 % higher than in SHF bioprocess. more...
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- 2018
34. Desorption of synthetic dye methylene blue from dye-adsorbed lignocellulosic biosorbent using organic solvents
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Bušić, Valentina, Velić, Natalija, Bunjik, Ivona, Martinović, Matea, Kezerle, Antonija, Pavlović, Hrvoje, Gašo-Sokač, Dajana, Radojčić, Redovniković I, Jakovljević T, Petravić, Tominac V, Panić M, Stojaković R, Erdec D, Radošević K, Gaurin, Sarček V, and Cvjetko Bubalo M more...
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eutectic solvents, extraction, polar protic solvents, methylene blue - Abstract
Adsorption is the method most often used for the removal of dyes from industrial effluents. However, due to the high-cost of conventional adsorbents, a wide range of lignocellulosic waste materials are being extensively investigated as a possible novel adsorbents. Many of them proved to be very effective. Dye-loaded lignocellulosic material cannot be directly discarded to the environment, so one of the alternatives is to ferment it using fungi that are capable of degrading the dye. For the purpose of future analyses of fermented dye-loaded lignocellulosic material, i.e. the quantification of the dye degradation extent, a series of organic solvents were used to extract the methylene blue (MB) adsorbed onto brewers’ spent grain (BSG). Both unfermented and fermented (solid-state fermentation using T. versicolor) dye-adsorbed BSG samples were tested. The individual solvents used in this study included nonpolar slovents (benzene, toluene, chloroform, dichloromethane and xylene), polar aprotic solvents (tetrahydrofuran, ethyl acetate, acetone, dimethylformamide, acetonitrile, dimethyl sulfoxide), and polar protic solvents (ethanol, methanol, amyl alcohol and water). Furthermore, a two-component solvent system (methanol : water 1 : 1) and three-component solvent system (methanol : chloroform : water 1 : 1 : 1) were also used. To stress the importance of the principles of green chemistry, ultrasonic-assisted extraction of MB from BSG with deep eutectic solvents (DES), choline chloride/hydrogen bond donor (ChCl/HBD) was also explored. The results showed that the highest desorption of MB from BSG was achieved using polar protic solvents, out of which methanol was the best. The lowest desorption was achieved using non-polar solvents. The results of the MB extraction from BSG using deep eutectic solvents indicated that some of the tested eutectic solvents could be used as an “green” alternative to organic solvents. However, they were far less efficient than the polar protic solvents. more...
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- 2018
35. Polyphenols recovery from dandelion leaves and roots assisted by high hydrostatic pressure (HPAE) and pressurized liquid extraction (PLE)
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Bursać Kovačević, D., Tenžera, D., Ćuk, A., Putnik, P., Dragović-Uzelac, Radojčić Redovniković, I., Jakovljević, T, Petravić Tominac, V., Panić, M., Stojaković, R., Erdec, D., Radošević, K., Gaurina Sarček, V., and Cvjetko Bubalo, M more...
- Subjects
dandelion ,high hydrostatic pressure extraction (HPAE) ,pressurized liquid extraction (PLE) - Abstract
Dandelion ( Taraxacum officinale) leaves and roots are good sources of health-promoting phytochemicals. Generally, applications of modern green extraction techniques, such as High Hydrostatic Pressure Assisted Extraction (HPAE) and Pressurized Liquid Extraction (PLE) showed significant advantages over conventional extractions in terms of less solvent and energy consumption, shorter extraction time, higher yields, etc. Thus, it could be useful for the effective extraction of various bioactive compounds from plant materials. In line with this, the aim of this study was to investigate the use of HPAE and PLE for the recovery of polyphenols from dandelion leaves and roots. Studied parameters for HPAE were: ethanol concentrations (50 and 70%), high pressures (300, and 500 MPa), times (5 and 15 min), and temperatures (22 and 60°C) PLE was conducted at constant static time (5 min) and pressure (10.34 MPa) by varying: ethanol concentrations (50 and 70%), temperatures (60, 80 and 100 °C), and number of extraction cycles (1, 2 and 3). Optimal HPAE parameters for the polyphenols recovery of dandelion leaves and roots was at 5 min, 60°C, and 500 MPa, with 70% ethanol (v/v) that was the most effective solvent for the leaves ; and 50% ethanol (v/v) for the dandelion root extract. Dandelion leaves extract was richer with polyphenols than the root extract (44.99 vs.11.46 mg GAE/g). With respect to PLE, 50% ethanol (v/v) was the most appropriate extraction solvent from both plant matrices. Moreover, optimal PLE conditions with the highest recovery (93.94 mg GAE/g) for the leaves were 60°C with 2 extraction cycles, while for the roots highest yields (5199 mg GAE/g) were achieved under higher temperature and longer extraction time (80°C and 3 extraction cycles). In conclusion, both extraction techniques were suitable for the efficient green recovery of polyphenols from dandelion leaves and roots. However, according to obtained results PLE performance has proven to be more suitable than the HPAE. Therefore, it should be recommended for the fast and effective polyphenols recovery from both, leaves and roots. more...
- Published
- 2018
36. Removal of Congo red from aqueous solution using lignocellulosic biosorbent and solid state fermentation of dye-adsorbed biosorbent
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Kezerle, Antonija, Velić, Natalija, Pavlović, Hrvoje, Velić, Darko, Hasenay, Damir, Kovačević, Davor, Radojčić Redovniković, I., Gaurina Srček, V., Radošević, K., and Jakovljević, T., Stojaković, R., Erdec Hendrih, D. more...
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adsorptive removal ,biosorbent ,Congo red ,decolourisation ,T. versicolor - Abstract
Brewers’ spent grain (BSG), being the food industry lignocellulosic waste, was tested for its ability to adsorb azo dye Congo red (CR) from aqueous solutions. Effects of the initial dye concentration (30, 50, 100 and 150 mg L-1), contact time, adsorbent concentration (5, 10, and 15 g L-1), and pH (7, 8, 9 and 10) on dye removal were investigated. Dye removal was rapid within the first 60 min of the contact time. The percentage of dye removal increased with the increase in the adsorbent concentration and decrease in the initial dye concentration. The increase in pH caused reduction of the percentage removal. The percentage of CR adsorptive removal after 240 min contact time was over 85% for all runs. Dye-adsorbed biosorbent was further used as a substrate for solid-state fermentation (SSF) by the white rot fungus Trametes versicolor CCBAS AG613. Prior to solid-state fermentation experiment four white rot fungi strains, Phanerochaete chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 and Ceriporiopsis subvermispora, were screened for their ability to decolourise CR during cultivation (9 days at 27 ⁰C) on agar plates containing dye at final concentrations of 50, 100 and 150 mg L-1. Radial growth and the zone of colour change on agar plates were measured (at two perpendicular directions) every three days. Fungus T. versicolor CCBAS AG613 showed the highest decolourisation index (decolourization diameter/mycelial diameter) and was chosen for further SSF experiment. SSF of dye-adsorbed BSG (250 g BSG soaked in 350 mL of 150 mgL-1 dye solution) was carried out in a horizontal cylindrical glass flask at an ambient temperature. Temperature and weight were monitored on-line, while moisture, protein content, and colour of samples were measured after 7, 14 and 21 days of fermentation. Continuous protein content increase and weight loss were observed as a result of fungal growth and material biodegradation. Complete decolourisation of samples was not observed. However, colour determination showed the continuous increase of total colour change and colour intensity change of fermented samples, compared to their abiotic controls. more...
- Published
- 2016
37. Application of innovative technologies for production of plant extracts as ingredients for functional foods
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Putnik, P., Bursać Kovačević, D., Dragović-Uzelac, Radojčić Redovniković, I., Radošević, K., Jakovljević, T, Stojaković, R., Gaurina Srček, V., and Erdec, D.
- Subjects
microwave extraction of Dalmatian sage ,cold atmospheric gas phase plasma extraction ,anthocyanins ,pomegranate ,chokeberry - Abstract
1) microwave extraction of Dalmatian sage (Salvia officinalis L.) extracts that aimed to evaluate the influence of solvents (30% ethanol/water, v/v ; 30% acetone/water, v/v ; water), time (3, 5, 7, 9, 10 min), temperature (30, 50, 60, 80 °C), and acidity (addition of 10 % HCl) on polyphenolic recovery. Total polyphenols were best extracted with 30% acetone/without HCl/T=80°C for 10 minutes. The main polyphenol, rosmarinic acid was best extracted with 30% ethanol/with HCl at T=80°C for 10 minutes ; 2) cold atmospheric gas phase plasma extraction of anthocyanins from pomegranate (Punica granatum L.) juice. Plasma treatment covered different operating settings: (i) time (3, 5, 7 min), (ii) juice volume (3, 4, 5 cm3), and (iii) plasma gas flow (0.75, 1, 1.25 dm3/min). Anthocyanins were best stable at: 3 min of treatment time, 5 cm3 sample volume, and 0.75dm3/min gas flow. Anthocyanin content was higher for 21-35% in plasma treated samples vs. fresh juices. In summary, it was shown that plasma treatment had positive influences on anthocyanins stability in cloudy pomegranate juice ; 3) cold atmospheric gas phase plasma of chokeberry juice (Aronia melancarpa) extracts that aimed to evaluate influence of different treatment times and juice volumes under constant gas flow (0.75 dm3min-1) on polyphenolic content. All samples were compared to the fresh and pasteurized chokeberry juice (80°C/2min). Plasma treatment better preserved hydroxycinnamic acids, but showed loss of anthocyanins. Pasteurisation negatively affected hydroxycinnamic acids, while flavonols and anthocyanins were well preserved. more...
- Published
- 2016
38. Mineral composition and heavy metals content of sweet chestnut fruits in five natural populations from central Croatia
- Author
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Poljak, Igor, Vahčić, Nada, Gačić, Milica, Ježić, Marin, Ćurković-Perica, Mirna, Idžojtić, Marilena, and Radojčić Redovniković, I., Radošević, K., Jakovljević, T., Stojaković, R., Gaurina Srček, V., Erdec Hendrih, D. more...
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fruits ,heavy metals ,mineral composition ,natural populations ,sweet chestnut - Abstract
In recent years the consumers have been showing an increased interest in chestnut fruits because of their nutritional qualities and potential beneficial health effects. Since the information about nutritional composition of the fruits from the natural populations is still limited the aim of this stud was to analyze their mineral content. Nuts were sampled in five natural populations, from the central area of sweet chestnut distribution range in Croatia. Each population was represented with five trees. Macro and micro elements (K, Ca, Mg, Na, Fe, Zn) and heavy metals (Pb, Cd, Hg, As) were determined by atomic absorption spectrophotometry using a Varian SpectrAA 220 device. Univariate and multivariate statistical techniques were used to evaluate the differences among populations. Chestnuts of all five populations contained K, Ca, Mg, Na, Fe, Zn in average 613.96, 35.17, 29.99, 3.69, 1.37 and 0.70 respectively. The obtained results for heavy metals content provided evidence that the concentrations were lower than the toxic law limits. An analysis of variance showed significant difference between populations regarding each mineral. The study also confirmed a trend of population variations according to the eco-geogrphic principle. more...
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- 2016
39. Cross-protection by conventional influenza vaccines
- Author
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Roos, A.L.E., Goudsmit, J., Radošević, K., Roozendaal, R., and Faculteit der Geneeskunde
- Abstract
In this thesis, we explore whether the protective efficacy of a trivalent virosomal seasonal influenza vaccine (TVV) can be broadened and thereby increase pandemic preparedness until more broadly protective influenza vaccines may become available. Chapter 2 examines the ability of a vaccination regimen comprising multiple immunizations to improve the cross-protective efficacy of TVV in mice. Chapter 3 explores whether priming a TVV with vaccine homologous HA DNA can improve its efficacy of inducing heterologous H1N1 and heterosubtypic H5N1 protection in mice. The cross-protective efficacy of the heterologous prime/boost regimen is evaluated in parallel with the multiple vaccination regimen identified in chapter 2. Chapter 4 investigates the ability of improving the cross-protective efficacy of TVV by adjuvating with Matrix M. The adjuvated vaccination regimen is evaluated for cross-protection against avian H5N1, H7N7 and H7N9 influenza viruses in both mice and ferrets. Leveraging two phase 1 clinical trials we in chapter 5-6 further evaluate the cross-reactivity and cross-protective efficacy of the humoral immune response elicited by seasonal and pandemic influenza vaccines in humans. Chapter 5 assesses the cross-protective efficacy of TVV in healthy adults. By making use of a novel human-to-mouse serum transfer and challenge model we evaluated the cross-protective efficacy of the humoral immune response induced by 1, 2 and 3x TVV in healthy adults. Chapter 6 further explores the role of ADCC in HA specific cross-reactivity and cross-protection induced by TVV and pandemic influenza vaccine in healthy adults. Chapter 7 comprises a summary and discussion of the findings presented in this thesis. The results are discussed in the context of the influenza vaccine field and the future perspective of broadly protective influenza vaccines. more...
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- 2016
40. In search for optimal virus strain for biological control of chestnut blight
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Nuskern, Lucija, Tkalec, Mirta, Ježić, Marin, Katanić, Zorana, Krstin, Ljiljana, Ćurković-Perica, Mirna, and Radojčić Redovniković, I., Radošević, K., Jakovljević, T., Stojaković, R., Gaurina Srček, V., Erdec Hendrih, D. more...
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fungi ,food and beverages ,biocontrol ,chestnut blight ,hypovirulence - Abstract
Cryphonectria parasitica (Murr.) Barr is an introduced phytopathogenic fungus and causal agent of chestnut blight, a devastating disease of chestnut trees. Since its introduction into Europe in the first half of the 20th century it has caused serious damage in the stands of European chestnut (Castanea sativa Mill.). After initial disease outbreak chestnut populations started to recover due to the natural biocontrol of C. parasitica with cointroduced dsRNA virus Cryphonectria hypovirus 1 (CHV1). CHV1 reduces the virulence of C. parasitica, a phenomenon called hypovirulence, thus enabling the recovery of chestnuts. Based on the genetic variability of European populations five subtypes of CHV1 have been defined, differing in their virulence towards the fungal host. In several European countries chestnut blight biocontrol by application of selected hypoviulent C. parasitica strains on active cankers is implemented. The success of biocontrol is limited by high diversity of fungal vegetative (vc) types which impedes the spread of the virus through C. parasitica populations. Thus the best practice approach in the biocontrol has to take into account specificities of local C. parasitica and CHV1 populations. The aim of the work was to investigate the effect of Croatian virus strains on different Croatian C. parasitica isolates in order to find optimal virus candidate for use in biocontrol treatments of chestnut blight in Croatia. Virus isolates were characterised as “strong” or “weak” based on their impact on fungal growth on chestnut stems. Then they were transferred into three fungal isolates of the same vc type by hyphal anastomosis. The effect of the virus on fungal radial growth in vitro and on the activity of fungal stress response enzymes catalase, glutathione S-transferase and superoxide dismutase was measured. The presence of CHV-1 in the C. parasitica resulted in reduced fungal growth in most virus-fungus combinations. The activities of fungal stress enzymes were affected by the presence of CHV-1, but the direction and intensity of the effects depended on virus and fungus isolate and their combination. Therefore, the “strength” of the virus is not solely determined by its subtype but is very strongly affected by both host and pathogen genotypes. This further confirms that possible biocontrol efforts have to take into account local specificities of C. parasitica and CHV1 populations. more...
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- 2016
41. Ecosystem services provided by alien invasive plant species
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Sladonja, Barbara, Poljuha, Danijela, Radojčić Redovniković, R., Radošević, K., Jakovljević, T., Gaurina Srček, V., and Erdec Hendrih, D.
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invasive species ,ecosystem service ,Ailanthus altissima ,management ,balance - Abstract
Alien invasive species are considered to be one of the largest threats to biodiversity globally, but have also a number of potential ecosystem services useful to human well-being. A balanced view of the negative impact on ecosystem and an overview on potential services provided by one of the most widespread invasive alien species in Europe and North America, Ailanthus altissima (Mill.) Swingle, is given in the present study. We have identified several ecosystem services such as pharmaceutical (human and agricultural use), nutritional (honey), energetic (fuelwood), regulating (erosion, land reclamation), cultural (ornamental) and their provision by A. altissima. Here we particularly present preliminary data on A. altissima extracts potential use as herbicide in urban areas and agriculture. On the other hand, alien invasive species can cause degradation of the services and this issue is also discussed. In conclusion, invasive risk management comprises balanced actions in prevention and removal of alien invasive species and possible uses in boosting ecosystem services. It is therefore necessary to explore all pro and contra elements related to particular species, and apply the most effective invasive risk management model based on systematic data. more...
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- 2016
42. Broadly protective influenza vaccines: Redirecting the antibody response through adjuvation
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Cox, Freek, Goudsmit, J., Radošević, K., Saeland, E., Faculteit der Geneeskunde, Goudsmit, Jaap, and Other departments
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virus diseases - Abstract
Influenza virus infections are responsible for significant morbidity worldwide and current vaccines have limited coverage, therefore it remains a high priority to develop broadly protective vaccines. With the discovery of broadly neutralizing antibodies (bnAbs) against influenza these vaccines became within reach. However, a basic understanding of the mechanisms by which bnAbs mediate protection is essential to effectively design the next generation of influenza vaccines. In this thesis we discuss the role of the Fc part of bnAbs in mediating antibody dependent cellular cytotoxicity (ADCC) and we demonstrate that additional interactions between HA on host cells and sialic acids on the surface of immune cells are required. Furthermore, we demonstrate that bnAbs in human serum samples induce cross-protection in influenza challenge models in mice. The potential to induce bnAbs against influenza by adjuvation of current influenza vaccines is discussed and the protective efficacy of the saponin-based adjuvant Matrix-M that in combination with a seasonal trivalent virosomal vaccine (TVV) was studied in mice and ferrets. In mice, protection against heterologous and avian H5N1 and H7N7 strains was achieved. In ferrets only partial protection against H5N1 was obtained but no protection against H7N7. We demonstrated that the protection against H5N1 in mice required both the humoral and cellular arms of the immune system. In summary, this thesis provides insight into the immunological mechanisms involved in broadly protective immunity and thereby contributes to the development of a universal influenza vaccine. more...
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- 2016
43. Formulation of Ready-to-Use Broccoli Extracts Rich in Polyphenols and Glucosinolates Using Natural Deep Eutectic Solvents.
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Karaula I, Vasung E, Damjanović A, Panić M, Radović M, Radošević K, Bagović Kolić M, Cvjetko Bubalo M, and Radojčić Redovniković I
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- Humans, Deep Eutectic Solvents chemistry, Antioxidants chemistry, Antioxidants pharmacology, Glucosinolates chemistry, Polyphenols chemistry, Brassica chemistry, Plant Extracts chemistry, Plant Extracts pharmacology
- Abstract
Broccoli is rich in biologically active compounds, especially polyphenols and glucosinolates, known for their health benefits. Traditional extraction methods have limitations, leading to a shift towards using natural deep eutectic solvents (NADESs) to create high-quality extracts with enhanced biological activity. This study focuses on preparing broccoli extracts in NADES, enriched with polyphenols and glucosinolates, without additional purification steps. Using the COSMOtherm software, the solubility of polyphenols and glucosinolates in NADESs was predicted, and five biocompatible betaine-based NADESs were prepared with glucose (B:Glc
1:1 and B:Glc5:2 ), sucrose (B:Suc), glycerol (B:Gly), and malic acid (B:MA) as hydrogen bond donors. The resulting extracts were assessed for total polyphenol and glucosinolate content, along with antioxidant capacity, using the ORAC assay. The results demonstrated that NADES extracts contained higher polyphenol content and exhibited enhanced antioxidant effects compared to the reference ethanol extract, with B:Glc1:1 extract showing the highest performance among all the extracts tested. On the other hand, the extract based on B:MA exhibited nearly six times higher total glucosinolate content compared to the ethanol extract. Additionally, polyphenols and glucosinolates were generally more stable in NADES extracts than in the reference solvent. Finally, the B:Glc1:1 extract, identified as optimal in terms of polyphenol and glucosinolate content and stability, exhibited mild stimulation of HaCaT cells growth and facilitated the wound-healing process. Through green chemistry parameter calculations, we demonstrated that the extraction of broccoli bioactives using B:Glc1:1 can be considered sustainable, underscoring the potential of NADESs for producing ready-to-use plant extracts. more...- Published
- 2024
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44. The Green Extraction of Blueberry By-Products: An Evaluation of the Bioactive Potential of the Anthocyanin/Polyphenol Fraction.
- Author
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Capaldi G, Aimone C, Calcio Gaudino E, Radošević K, Bagović M, Grillo G, and Cravotto G
- Subjects
- Humans, HeLa Cells, Microwaves, Monophenol Monooxygenase antagonists & inhibitors, Monophenol Monooxygenase metabolism, Anthocyanins pharmacology, Anthocyanins isolation & purification, Anthocyanins chemistry, Blueberry Plants chemistry, Plant Extracts pharmacology, Plant Extracts chemistry, Antioxidants pharmacology, Antioxidants chemistry, Antioxidants isolation & purification, Polyphenols pharmacology, Polyphenols isolation & purification, Polyphenols chemistry
- Abstract
In the context of a circular economy, this study explores the valorization of blueberry pomace (BP) as a source of bioactive compounds using sustainable extraction methods. Microwave-assisted extraction (MAE) and microwave-assisted subcritical water extraction (MASWE) were employed to obtain two distinct fractions: MAE 1° and MASWE 2°. The first extract, MAE 1°, obtained at 80 °C, had a high total anthocyanin content (21.96 mg
Cya-3-glu /gextract ), making it suitable as a natural pigment. Additionally, MAE 1° exhibited significant enzyme inhibition, particularly against α-amylase and β-glucosidase, suggesting potential anti-diabetic and anti-viral applications. The second extract, MASWE 2°, obtained at 150 °C, contained a higher total phenolic content (211.73 mgGAE /gextract ) and demonstrated stronger antioxidant activity. MASWE 2° showed greater inhibition of acetylcholinesterase and tyrosinase, indicating its potential for use in Alzheimer's treatment, skincare, or as a food preservative. MASWE 2° exhibited cytotoxicity against HeLa cells and effectively mitigated H2 O2 -induced oxidative stress in HaCat cells, with MAE 1° showing similar but less pronounced effects. A tested formulation combining MAE 1° and MASWE 2° extracts in a 3:2 ratio effectively enhanced anthocyanin stability, demonstrating its potential as a heat-stable pigment. The extract characteristics were compared with a conventional method (MeOH-HCl in reflux condition), and the protocol's sustainability was assessed using several green metric tools, which provided insights into its environmental impact and efficiency. more...- Published
- 2024
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45. Unlocking the Bioactive Potential of Pomegranate Peels: A Green Extraction Approach.
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Grillo G, Capaldi G, Radošević K, Jakopović Ž, Markov K, Brncic M, Gallina L, Calcio Gaudino E, and Cravotto G
- Abstract
Pomegranate ( Punica granatum L.) is well known for its high content of bioactives, including polyphenols, flavonoids, and tannins, which have been shown to exhibit a wide range of biological activities, such as antioxidant, antimicrobial, and anticancer effects. It is worth noting that the majority of these molecules are found in the peels, which are usually disposed of after processing, causing a significant amount of waste, amounting to more than 3.6 million t/y. This work investigates microwave-assisted extraction (MAE) in water for the recovery of antioxidants from pomegranate peels (PP), including the optimisation of temperature and extraction times. The total phenolic, anthocyanin, flavonoid, and tannin contents of the recovered extracts were determined, as well as their antioxidant activities, which were found to be 356.35 mgGAE/gExtr, 303.97 µgCy3G/gExtr, 37.28 mgQE/gExtr, 56.48 mgGAE/gExtr, and 5.72 mmolTE/gExtr, respectively (according to the adopted reference). All results were compared with those obtained using a conventional protocol. In addition, the potential for water recycling by means of downstream nanofiltration in optimised MAE was investigated, leading to overall water reuse of approx. 75%. Power consumption (20.92 W/mgGAE) and common green metrics, Reaction Mass Efficiency (RME), E-Factor, and the Process Mass Intensiti/efficiency (PMI, PME), were considered in evaluating the proposed PP valorisation strategy. Finally, the biological activities of the main products were assessed. The antimicrobial properties of the PP extracts against three Gram-positive and three Gram-negative bacteria and their antiproliferative activity towards human cancer cells were tested. S. aureus bacteria was the most susceptible to the PP extracts. All tested products displayed antiproliferative activity against HeLa cells when higher concentrations were tested, with D-PP/NF (obtained from dried PP and sequential nanofiltration) being the most effective. This result was also confirmed via clonogenic analysis, which generally indicated the possible anti-cancer activity of pomegranate peel extracts obtained using this green approach. more...
- Published
- 2023
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46. Novel ferrocene-containing triacyl derivative of resveratrol improves viability parameters in ovary cells.
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Kmetič I, Murati T, Kovač V, Jurčević IL, Šimić B, Radošević K, and Miletić M
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- Resveratrol pharmacology, Reactive Oxygen Species metabolism, Metallocenes pharmacology, Apoptosis, Antioxidants metabolism, Stilbenes pharmacology
- Abstract
Besides the use of resveratrol as a drug candidate, there are obstacles mainly due to its poor pharmacokinetic properties. Numerous studies are being conducted on the synthesis of resveratrol derivatives that exhibit enhanced biological activity. The aim of our research was to investigate activity of the newly synthesized ferrocene-containing triacyl derivative of resveratrol to achieve cell protection from endo/exogenous ROS and reduction in cell death by assessing multiple endpoints. Our research showed that both resveratrol and the resveratrol derivatives (1-100 μM) lower the levels of ROS in CHO-K1 cells. Resveratrol at doses <20 μM had little or no effect on cell proliferation, while at higher doses, a significant inhibitory effect on cell proliferation and viability has been noticed. The activity of the new derivative was significantly altered compared to resveratrol-cellular viability was not suppressed regardless of the concentration applied, and the extent of apoptosis was low. In summary, the new ferrocene-resveratrol derivative has the potential to protect cells from oxidative stress due to its low cytotoxicity and retained antioxidant properties, whereas caution should be exercised with resveratrol at higher doses, as it significantly impairs cell viability and induces cell death. By linking ROS to specific diseases (relevance in neurodegenerative, cardiovascular, and neoplastic diseases), we can assume that the new resveratrol derivative can prevent or alleviate the mentioned disorders. Furthermore, recognition of the resveratrol derivative as an anti-apoptotic chemical could be useful in the cultivation of various cell lines on a large scale in the industrial biotechnology., (© 2023 John Wiley & Sons Ltd.) more...
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- 2023
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47. Prediction of pH Value of Aqueous Acidic and Basic Deep Eutectic Solvent Using COSMO-RS σ Profiles' Molecular Descriptors.
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Panić M, Radović M, Cvjetko Bubalo M, Radošević K, Rogošić M, Coutinho JAP, Radojčić Redovniković I, and Jurinjak Tušek A
- Subjects
- Hydrogen-Ion Concentration, Models, Theoretical, Solvents chemistry, Deep Eutectic Solvents, Neural Networks, Computer
- Abstract
The aim of this work was to develop a simple and easy-to-apply model to predict the pH values of deep eutectic solvents (DESs) over a wide range of pH values that can be used in daily work. For this purpose, the pH values of 38 different DESs were measured (ranging from 0.36 to 9.31) and mathematically interpreted. To develop mathematical models, DESs were first numerically described using σ profiles generated with the COSMOtherm software. After the DESs’ description, the following models were used: (i) multiple linear regression (MLR), (ii) piecewise linear regression (PLR), and (iii) artificial neural networks (ANNs) to link the experimental values with the descriptors. Both PLR and ANN were found to be applicable to predict the pH values of DESs with a very high goodness of fit (R2independent validation > 0.8600). Due to the good mathematical correlation of the experimental and predicted values, the σ profile generated with COSMOtherm could be used as a DES molecular descriptor for the prediction of their pH values. more...
- Published
- 2022
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48. Conformational Preferences and Antiproliferative Activity of Peptidomimetics Containing Methyl 1'-Aminoferrocene-1-carboxylate and Turn-Forming Homo- and Heterochiral Pro-Ala Motifs.
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Kovačević M, Čakić Semenčić M, Radošević K, Molčanov K, Roca S, Šimunović L, Kodrin I, and Barišić L
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Crystallography, X-Ray methods, Dipeptides chemistry, Dipeptides pharmacology, HeLa Cells, Humans, MCF-7 Cells, Peptides chemistry, Peptides pharmacology, Protein Structure, Secondary, Stereoisomerism, Carboxylic Acids chemistry, Carboxylic Acids pharmacology, Cell Proliferation drug effects, Ferrous Compounds chemistry, Ferrous Compounds pharmacology, Peptidomimetics chemistry, Peptidomimetics pharmacology
- Abstract
The concept of peptidomimetics is based on structural modifications of natural peptides that aim not only to mimic their 3D shape and biological function, but also to reduce their limitations. The peptidomimetic approach is used in medicinal chemistry to develop drug-like compounds that are more active and selective than natural peptides and have fewer side effects. One of the synthetic strategies for obtaining peptidomimetics involves mimicking peptide α-helices, β-sheets or turns. Turns are usually located on the protein surface where they interact with various receptors and are therefore involved in numerous biological events. Among the various synthetic tools for turn mimetic design reported so far, our group uses an approach based on the insertion of different ferrocene templates into the peptide backbone that both induce turn formation and reduce conformational flexibility. Here, we conjugated methyl 1'-aminoferrocene-carboxylate with homo- and heterochiral Pro-Ala dipeptides to investigate the turn formation potential and antiproliferative properties of the resulting peptidomimetics 2 - 5 . Detailed spectroscopic (IR, NMR, CD), X-ray and DFT studies showed that the heterochiral conjugates 2 and 3 were more suitable for the formation of β-turns. Cell viability study, clonogenic assay and cell death analysis showed the highest biological potential of homochiral peptide 4 . more...
- Published
- 2021
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49. COSMOtherm as an Effective Tool for Selection of Deep Eutectic Solvents Based Ready-To-Use Extracts from Graševina Grape Pomace.
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Panić M, Gunjević V, Radošević K, Cvjetko Bubalo M, Ganić KK, and Redovniković IR
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- Catechin analysis, Cell Survival drug effects, HaCaT Cells drug effects, Humans, Plant Extracts pharmacology, Polyphenols isolation & purification, Water analysis, Software, Solvents chemistry, Vitis chemistry, Waste Products analysis
- Abstract
The aim of this work is to develop an industrially suitable process for the sustainable waste disposal in wine production. The proposed process involves the development of an environmentally friendly method for the isolation of biologically active compounds from Graševina grape pomace according to the green extraction principles, in order to obtain a ready-to-use extract. In this process, deep eutectic solvents (DES) were used as extraction solvents. Aiming to save time in selecting the optimal DES that would provide the most efficient Graševina pomace polyphenols extraction, the user-friendly software COSMOtherm was used and 45 DES were screened. Moreover, the prepared extracts were chemically and biologically characterized to confirm their safety for human application. Computational and experimental results proved the applicability of COSMOtherm in the selection of the optimal DES for the environmentally friendly preparation of the ready-to-use extract from Graševina grape pomace with expected application in the cosmetic industry. more...
- Published
- 2021
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50. L-Carnitine Tartrate Downregulates the ACE2 Receptor and Limits SARS-CoV-2 Infection.
- Author
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Bellamine A, Pham TNQ, Jain J, Wilson J, Sahin K, Dallaire F, Seidah NG, Durkee S, Radošević K, and Cohen ÉA
- Subjects
- Adult, Aged, Angiotensin-Converting Enzyme 2 blood, Animals, COVID-19 metabolism, Carnitine pharmacology, Cell Line, Cell Survival drug effects, Female, Furin blood, Furin metabolism, Humans, Inflammation metabolism, Male, Middle Aged, Rats, SARS-CoV-2, Serine Endopeptidases blood, Serine Endopeptidases metabolism, Tartrates pharmacology, Young Adult, Angiotensin-Converting Enzyme 2 metabolism, Carnitine administration & dosage, Tartrates administration & dosage, COVID-19 Drug Treatment
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for one of the worst pandemics in modern history. Several prevention and treatment strategies have been designed and evaluated in recent months either through the repurposing of existing treatments or the development of new drugs and vaccines. In this study, we show that L-carnitine tartrate supplementation in humans and rodents led to significant decreases of key host dependency factors, notably angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and Furin, which are responsible for viral attachment, viral spike S-protein cleavage, and priming for viral fusion and entry. Interestingly, pre-treatment of Calu-3, human lung epithelial cells, with L-carnitine tartrate led to a significant and dose-dependent inhibition of the infection by SARS-CoV-2. Infection inhibition coincided with a significant decrease in ACE2 mRNA expression levels. These data suggest that L-carnitine tartrate should be tested with appropriate trials in humans for the possibility to limit SARS-CoV-2 infection. more...
- Published
- 2021
- Full Text
- View/download PDF
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