1. Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All
- Author
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Jelena Arsenijević, Jelena Živković, Đorđe Moravčević, Slavica Ražić, Radosav Jevđović, Ivan Šoštarić, Milica Drobac, and Zoran Maksimović
- Subjects
0106 biological sciences ,Cultivation ,Citral ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,Fertilizer ,Apigenin glycosides ,law ,Caffeic acid ,Essential oil ,2. Zero hunger ,Chemotype ,biology ,010405 organic chemistry ,Rosmarinic acid ,Thyme ,biology.organism_classification ,0104 chemical sciences ,Horticulture ,chemistry ,Composition (visual arts) ,Lamiaceae ,Thymus pannonicus ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62–1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49–1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90–81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15–184.94 mg/g and 5.08–15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61–98.75 mg/g and 1.17–1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.
- Published
- 2019
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