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3 results on '"Rafael Rafael Amorim Cavalcanti de Siqueira"'

1. A Fast Method for DEFB1-44C/G SNP Genotyping in Brazilian Patients with Periodontitis

Author

Renata Cimões, Rafael Rafael Amorim Cavalcanti de Siqueira, Sergio Crovella, Paulo Roberto Eleutério de Souza, and Nikos Donos

Subjects
Defensins, Antimicrobial Cationic Peptides, DEFB1 protein, human, Epithelial Cells, Diabetes Complications, Periodontitis, Dentistry, RK1-715
Abstract

Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

Published
2014
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2. Brza metoda za genotipiziranje DEFB1 - 44C/G SNP-a kod brazilskih pacijenata s parodontitisom

Author

Renata Cimões, Rafael Rafael Amorim Cavalcanti de Siqueira, Sergio Crovella, Paulo Roberto Eleutério de Souza, Nikos Donos, Renata Cimões, Rafael Rafael Amorim Cavalcanti de Siqueira, Sergio Crovella, Paulo Roberto Eleutério de Souza, and Nikos Donos

Abstract

Svrha: Defensini su kationski antimikrobni peptidi koji se pojavljuju u epitelnim stanicama. Imaju antimikrobna, antifungalna i antivirusna svojstva te su prirođena komponenta imunosnog odgovora. Jedna od hipoteza jest da peptidi štite usnu šupljinu. U ovom istraživanju procijenili smo polimorfizam gena DEFB1 kod dijabetičara s parodontitisom ili bez njega i rezultate usporedili sa zdravom kontrolnom skupinom. Materijali i metode: Koristili smo se testom Hairpin-Shaped Primer (HP) kako bismo istražili rasprostranjenost - 44 C/G SNP-a (rsl1800972) u 119 uzoraka ljudske DNK dobivene od dijabetičara i zdravih pacijenata. Rezultati: Rezultati su pokazali da između grupa nema razlika u rasprostranjenosti, te da je kod dijabetičara s parodontitisom češći homozigotni mutant. Zaključak: Potrebna su daljnja istraživanja kako bi se otkrila uloga polimorfizma DEFB1 kod dijabetičara s parodontitisom te utjecaj peptida na parodontne patogene., Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

Published
2014

3. A Fast Method for DEFB1-44C/G SNP Genotyping in Brazilian Patients with Periodontitis

Author

Sergio Crovella, Rafael Amorim Cavalcanti de Siqueira, Nikos Donos, Paulo Roberto Eleutério de Souza, Renata Cimões, Renata, Cimõe, Rafael Rafael Amorim Cavalcanti de, Siqueira, Crovella, Sergio, Paulo Roberto Eleutério de, Souza, and Nikos, Donos

Subjects
Mutant, DEFB1 gene, defenzini, kationski antimikrobni peptidi, DEFB1 bjelančevina, humana, epitelne stanice, dijabetes, komplikacije, parodontitis, Peptide, Oral cavity, Defensins, Diabetes Complications, genetic polymorphisms, medicine, Cationic Antimicrobial Peptides, SNP, Antimicrobial Cationic Peptides, DEFB1 protein, human, Epithelial Cells, Periodontitis, General Dentistry, chemistry.chemical_classification, Innate immune system, business.industry, medicine.disease, SNP genotyping, defensins, lcsh:RK1-715, chemistry, lcsh:Dentistry, Immunology, business, defensin, Research Article
Abstract

Svrha: Defensini su kationski antimikrobni peptidi koji se pojavljuju u epitelnim stanicama. Imaju antimikrobna, antifungalna i antivirusna svojstva te su prirođena komponenta imunosnog odgovora. Jedna od hipoteza jest da peptidi štite usnu šupljinu. U ovom istraživanju procijenili smo polimorfizam gena DEFB1 kod dijabetičara s parodontitisom ili bez njega i rezultate usporedili sa zdravom kontrolnom skupinom. Materijali i metode: Koristili smo se testom Hairpin-Shaped Primer (HP) kako bismo istražili rasprostranjenost - 44 C/G SNP-a (rsl1800972) u 119 uzoraka ljudske DNK dobivene od dijabetičara i zdravih pacijenata. Rezultati: Rezultati su pokazali da između grupa nema razlika u rasprostranjenosti, te da je kod dijabetičara s parodontitisom češći homozigotni mutant. Zaključak: Potrebna su daljnja istraživanja kako bi se otkrila uloga polimorfizma DEFB1 kod dijabetičara s parodontitisom te utjecaj peptida na parodontne patogene., Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

Published
2014

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