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DNA extraction from insects is an initial and critical step that might affect the molecular taxonomy. There are several protocols for efficient DNA extraction published by researchers, which might be helpful for others working in the relevant field. There are also some commercial kits optimized to extract DNA even from low volumes or less amount of tissue. Research has conventionally showed that Phenol: chloroform: Isoamyl alcohol (PCI) method gives good yield and is cost effective as compared to other methodologies, especially commercial kits. Hereby is the CTAB and PCI protocol, which gives good yield from insect tissue preferably legs, head and wings (avoid using whole body).

DNA barcodes analyzed by using relevant techniques provide an imperative approach towards validation of prevailing taxa and putative species. Here, molecular methods were used for assessment of 246 barcodes belonging to 81 fish species from northern Western Ghats of India, using, Barcode gap analysis, barcode index number, automatic barcode gap discovery, Poisson tree processes and general mixed Yule-coalescent, these methods had their potential to discriminate 97.53%, 93.90% 95.06%, 93.82% and 92.59% of species respectively. But, some of them tended to estimate the inconsistent number of species leading to discrepancies between the morphological concept and inference from molecular phylogenetic reconstructions. So, we took a standard approach to recognize those methods that produced consistent results, three of five such methods were identified that revealed three hidden cryptic species complexes in Monopterus indicus, Parambassis ranga and Systomus sarana. Further, to validate these three genetically diverged species, we used diagnostic character based approach along with nine unidentified species through BLOG and WEKAs SMO classifier. Those methods were unable to identify these species, which might be due to the limited number of specimens used for the analysis. This is the first effort to generate the DNA barcode reference library of freshwater fishes from northern Western Ghats of India, one of the world’s biodiversity hotspots. These barcodes when analyzed through the defined workflow, will provide valuable measures to prove the efficiency of molecular species delimitation methods in taxonomic discrimination which aid conservation of biodiversity.

This article emphasizes available genomic resources at the global databases National Center for Biotechnology Information (NCBI) GenBank, Gramene and Phytozome for the selected 378 plant taxa of the United Arab Emirates (UAE). Germplasm of these species was collected and banked at the Sharjah Seed Bank and Herbarium (SSBH) along with their related information on habit, habitat and occurrence. The occurrence statistics exhibits almost 19.84% species under rare-to-very rare category, the GenBank search statistics for this category indicates 17.72% species as studied and 2.11% as not studied. Overall, from the global search statistics for 378 plant species, it seems that about 40 (10.58%) species remained unstudied. Most of the unstudied species were herbaceous plants belonging to the mountainous habitat. Moreover, full genomes were recorded for 7 species at NCBI GenBank, 2 species at Phytozome and 1 species at Gramene database. The local search statistics (for UAE) exhibits about 10.58% of the flora that still remained unstudied and only 11 (2.90%) of the recorded species were having genomic information at NCBI GenBank. It is necessary to prioritize studies on such species that could provide valuable insight on their genetic composition in order to understand their adaptation to the natural environment. At present, the SSBH is cataloguing UAE’s flora using core barcode and assisted markers that could provide a robust DNA barcode library for native plants of UAE. Our study appeals researchers to recognize and prioritize the species that need attention to enrich their genomic resources at the global databases by supporting nucleotide libraries with their conspecifics. At present, genomic resources for UAE plant taxa are limited, but with the advent of low-cost sequencing technologies these resources would flourish in the near future. Nevertheless, the information generated through genomic studies could be utilized for conservation and management of threatened and endangered plant species, Crop Wild Relatives and medicinal plants. We hope this article will promote interest in conducting additional studies in genomics of desert plants by encouraging researchers to participate in this emerging field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13205-019-1855-9) contains supplementary material, which is available to authorized users.

The Arabian Peninsula is known to have a comprehensive and rich endowment of unique and genetically diverse plant genetic resources. Analysis and conservation of biological diversity is a crucial issue to the whole Arabian Peninsula. The rapid and accurate delimitation and identification of a species is crucial to genetic diversity analysis and the first critical step in the assessment of distribution, population abundance and threats related to a particular target species. During the last two decades, classical strategies of evaluating genetic variability, such as morphology and physiology, have been greatly complemented by phylogenetic, taxonomic, genetic diversity and breeding research molecular studies. At present, initiatives are taking place around the world to generate DNA barcode libraries for vascular plant flora and to make these data available in order to better understand, conserve and utilize biodiversity. The number of herbarium collection-based plant evolutionary genetics and genomics studies being conducted has been increasing worldwide. The herbaria provide a rich resource of already preserved and identified material, and these as well as freshly collected samples from the wild can be used for creating a reference DNA barcode library for the vascular plant flora of a region. This review discusses the main molecular and genomic techniques used in plant identification and biodiversity analysis. Hence, we highlight studies emphasizing various molecular techniques undertaken during the last 10 years to study the plant biodiversity of the Arabian Peninsula. Special emphasis on the role of DNA barcoding as a powerful tool for plant biodiversity analysis is provided, along with the crucial role of herbaria in creating a DNA barcode library.

Mangroves are salt-tolerant forest ecosystems of tropical and subtropical intertidal regions. They are among most productive, diverse, biologically important ecosystem and inclined toward threatened system. Identification of mangrove species is of critical importance in conserving and utilizing biodiversity, which apparently hindered by a lack of taxonomic expertise. In recent years, DNA barcoding using plastid markers rbcL and matK has been suggested as an effective method to enrich traditional taxonomic expertise for rapid species identification and biodiversity inventories. In the present study, we performed assessment of available 14 mangrove species of Goa, west coast India based on core DNA barcode markers, rbcL and matK. PCR amplification success rate, intra- and inter-specific genetic distance variation and the correct identification percentage were taken into account to assess candidate barcode regions. PCR and sequence success rate were high in rbcL (97.7 %) and matK (95.5 %) region. The two candidate chloroplast barcoding regions (rbcL, matK) yielded barcode gaps. Our results clearly demonstrated that matK locus assigned highest correct identification rates (72.09 %) based on TaxonDNA Best Match criteria. The concatenated rbcL + matK loci were able to adequately discriminate all mangrove genera and species to some extent except those in Rhizophora, Sonneratia and Avicennia. Our study provides the first endorsement of the species resolution among mangroves using plastid genes with few exceptions. Our future work will be focused on evaluation of other barcode markers to delineate complete resolution of mangrove species and identification of putative hybrids. Electronic supplementary material The online version of this article (doi:10.1186/s40064-016-3191-4) contains supplementary material, which is available to authorized users.

This study describes the species diversity of fishes of the Narmada River in India. A total of 820 fish specimens were collected from 17 sampling locations across the whole river basin. Fish were taxonomically classified into one of 90 possible species based on morphological characters, and then DNA barcoding was employed using COI gene sequences as a supplemental identification method. A total of 314 different COI sequences were generated, and specimens were confirmed to belong to 85 species representing 63 genera, 34 families and 10 orders. Findings of this study include the identification of five putative cryptic or sibling species and 43 species not previously known from the Narmada River basin. Five species are endemic to India and three are introduced species that had not been previously reported to occur in the Narmada River. Conversely, 43 species previously reported to occur in the Narmada were not found. Genetic diversity and distance values were generated for all of the species within genera, families and orders using Kimura's 2 parameter distance model followed by the construction of a Neighbor Joining tree. High resolution clusters generated in NJ trees aided the groupings of species corresponding to their genera and families which are in confirmation to the values generated by Automatic Barcode Gap Discovery bioinformatics platform. This aided to decide a threshold value for the discrimination of species boundary from the Narmada River. This study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within complex ecosystems such as the Narmada River.

India’s third longest river, the Narmada, is studied here for the potential effects on native fish populations of river fragmentation due to various barriers including dams and a waterfall. The species we studied include a cyprinid fish, Catla catla, and a mastacembelid, Mastacembelus armatus, both of which are found in the Narmada. Our goal was to use DNA sequence information from the D-loop region of the mitochondrial DNA to explore how this fragmentation could impact the genetic structure of these fish populations. Our results clearly show that these barriers can contribute to the fragmentation of the genetic structure of these fish communities, Furthermore, these barriers enhance the effects of natural isolation by distance and the asymmetry of dispersal flows. This may be a slow process, but it can create significant isolation and result in genetic disparity. In particular, populations furthest upstream having low migration rates could be even more subject to genetic impoverishment. This study serves as a first report of its kind for a river system on the Indian subcontinent. The results of this study also emphasize the need for appropriate attention towards the creation of fish passages across the dams and weirs that could help in maintaining biodiversity. Electronic supplementary material The online version of this article (doi:10.1186/2193-1801-3-385) contains supplementary material, which is available to authorized users.