1. Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt
- Author
-
Milind S. Patole, Rahul Datta, R. Z. Sayyed, Abdallah M. Elgorban, Hind A. AL-Shwaiman, Sham Diwanay, Manal M. Alkhulaifi, Subhan Danish, and Rajan A. Walhe
- Subjects
0106 biological sciences ,Geography, Planning and Development ,TJ807-830 ,Lactobacillus pentosus ,Management, Monitoring, Policy and Law ,Biology ,TD194-195 ,01 natural sciences ,Renewable energy sources ,law.invention ,vitamin B12 production ,03 medical and health sciences ,Probiotic ,chemistry.chemical_compound ,law ,010608 biotechnology ,GE1-350 ,Vitamin B12 ,Food science ,Fermentation in food processing ,yoghurt ,030304 developmental biology ,0303 health sciences ,Environmental effects of industries and plants ,Renewable Energy, Sustainability and the Environment ,Cholesterol ,food and beverages ,biology.organism_classification ,Lactic acid ,cholesterol reduction ,Environmental sciences ,chemistry ,Enterococcus faecium ,Bacteria - Abstract
The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.
- Published
- 2021