1. RNA-Seq explores the functional role of the fibroblast growth factor 10 gene in bovine adipocytes differentiation
- Author
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Nurgulsim Kaster, Rajwali Khan, Ijaz Ahmad, Kazhgaliyev Nurlybay Zhigerbayevich, Imbay Seisembay, Akhmetbekov Nurbolat, Shaikenova Kymbat Hamitovna, Omarova Karlygash Mirambekovna, Makhanbetova Aizhan Bekbolatovna, Tlegen Garipovich Amangaliyev, Ateikhan Bolatbek, Titanov Zhanat Yeginbaevich, Shakoor Ahmad, Zan Linsen, and Begenova Ainagul Baibolsynovna
- Subjects
bovine adipocytes ,gene ,intramuscular fat ,rna sequencing ,Zoology ,QL1-991 - Abstract
Objective The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p
- Published
- 2024
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