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2. In mice and humans, brain microvascular contractility matures postnatally

Author

Slaoui, Leila, Gilbert, Alice, Rancillac, Armelle, Delaunay-Piednoir, Barbara, Chagnot, Audrey, Gerard, Quentin, Letort, Gaëlle, Mailly, Philippe, Robil, Noémie, Gelot, Antoinette, Lefebvre, Mathilde, Favier, Maryline, Dias, Karine, Jourdren, Laurent, Federici, Laetitia, Auvity, Sylvain, Cisternino, Salvatore, Vivien, Denis, Cohen-Salmon, Martine, and Boulay, Anne-Cécile

Published
2023
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3. Impaired neurovascular coupling in the APPxPS1 mouse model of Alzheimer’s disease

Author

Geoffroy, Hélène, Rossier, Jean, and Rancillac, Armelle

Subjects
Quantitative Biology - Neurons and Cognition
Abstract

The tight coupling between neuronal activity and the local increase of blood flow termed neurovascular coupling is essential for normal brain function. This mechanism of regulation is compromised in Alzheimer's Disease (AD). In order to determine whether a purely vascular dysfunction or a neuronal alteration of blood vessels diameter control could be responsible for the impaired neurovascular coupling observed in AD, blood vessels reactivity in response to different pharmacological stimulations was examined in double transgenic APPxPS1 mice model of AD. Blood vessels movements were monitored using infrared videomicroscopy ex vivo, in cortical slices of 8 month-old APPxPS1 and wild type (WT) mice. We quantified vasomotor responses induced either by direct blood vessel stimulation with a thromboxane A 2 analogue, the U46619 (9,11-dideoxy-11a,9a-epoxymethanoprostaglandin F2) or via the stimulation of interneurons with the nicotinic acetylcholine receptor (nAChRs) agonist DMPP (1,1-Dimethyl-4-phenylpiperazinium iodide). Using both types of stimulation, no significant differences were detected for the amplitude of blood vessel diameter changes between the transgenic APPxPS1 mice model of AD and WT mice, although the kinetics of recovery were slower in APPxPS1 mice. We find that activation of neocortical interneurons with DMPP induced both vasodilation via Nitric Oxide (NO) release and constriction via Neuropeptide Y (NPY) release. However, we observed a smaller proportion of reactive blood vessels following a neuronal activation in transgenic mice compared with WT mice. Altogether, these results suggest that in this mouse model of AD, deficiency in the cortical neurovascular coupling essentially results from a neuronal rather than a vascular dysfunction., Comment: Current Alzheimer Research, Bentham Science Publishers, 2012

Published
2019

8. In mice and humans, brain microvascular contractility matures postnatally

Author

Slaoui, Leila, primary, Gilbert, Alice, additional, Rancillac, Armelle, additional, Delaunay-Piednoir, Barbara, additional, Chagnot, Audrey, additional, Gerard, Quentin, additional, Letort, Gaëlle, additional, Mailly, Philippe, additional, Robil, Noémie, additional, Gelot, Antoinette, additional, Lefebvre, Mathilde, additional, Favier, Maryline, additional, Dias, Karine, additional, Jourdren, Laurent, additional, Federici, Laetitia, additional, Auvity, Sylvain, additional, Cisternino, Salvatore, additional, Vivien, Denis, additional, Cohen-Salmon, Martine, additional, and Boulay, Anne-Cécile, additional

Published
2022
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9. Rôle du BDNF et des Interactions Neurogliales dans la Régulation du Sommeil Lent

Author

Walter, Augustin, Lecoin, Laure, Rezaig, Fayçal, Chauveau, Frédéric, Rouach, Nathalie, Rancillac, Armelle, Center for Interdisciplinary Research in Biology, College de France, CNRS, INSERM, PSL Research University, 75231 Paris, France. (CIRB), Institut de Recherche Biomédicale des Armée [Brétigny/Orge] (IRBA), and Rancillac, Armelle

Subjects
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

11. In mice and humans, brain microvascular contractility matures postnatally Brain microvessel post-natal maturation

Author

Slaoui, Leila, primary, Gilbert, Alice, additional, Rancillac, Armelle, additional, Chagnot, Audrey, additional, Federici, Laetitia, additional, Gerard, Quentin, additional, Gelot, Antoinette, additional, Becmeur-Lefebvre, Mathilde, additional, Favier, Maryline, additional, Robil, Noémie, additional, Letort, Gaëlle, additional, Dias, Karine, additional, Jourdren, Laurent, additional, Mailly, Philippe, additional, Auvity, Sylvain, additional, Cisternino, Salvatore, additional, Vivien, Denis, additional, Cohen-Salmon, Martine, additional, and Boulay, Anne-Cécile, additional

Published
2022
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13. In Mice and Humans, Brain Vascular Barrier Homeostasis and Contractility Are Acquired Postnatally

Author

Slaoui, Leila, primary, Gilbert, Alice, additional, Federici, Laetitia, additional, Rancillac, Armelle, additional, Chagnot, Audrey, additional, Gerard, Quentin, additional, Gelot, Antoinette, additional, Favier, Maryline, additional, Robil, Noémie, additional, Letort, Gaëlle, additional, Dias, Karine, additional, Jourdren, Laurent, additional, Mailly, Philippe, additional, Auvity, Sylvain, additional, Vivien, Denis, additional, Cisternino, Salvatore, additional, Cohen-Salmon, Martine, additional, and Boulay, Anne-Cécile, additional

Published
2022
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14. Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit

Author

Gilbert, Alice, primary, Elorza-Vidal, Xabier, additional, Rancillac, Armelle, additional, Chagnot, Audrey, additional, Yetim, Mervé, additional, Hingot, Vincent, additional, Deffieux, Thomas, additional, Boulay, Anne-Cécile, additional, Alvear-Perez, Rodrigo, additional, Cisternino, Salvatore, additional, Martin, Sabrina, additional, Taïb, Sonia, additional, Gelot, Aontoinette, additional, Mignon, Virginie, additional, Favier, Maryline, additional, Brunet, Isabelle, additional, Declèves, Xavier, additional, Tanter, Mickael, additional, Estevez, Raul, additional, Vivien, Denis, additional, Saubaméa, Bruno, additional, and Cohen-Salmon, Martine, additional

Published
2021
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15. Author response: Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit

Author

Gilbert, Alice, primary, Elorza-Vidal, Xabier, additional, Rancillac, Armelle, additional, Chagnot, Audrey, additional, Yetim, Mervé, additional, Hingot, Vincent, additional, Deffieux, Thomas, additional, Boulay, Anne-Cécile, additional, Alvear-Perez, Rodrigo, additional, Cisternino, Salvatore, additional, Martin, Sabrina, additional, Taïb, Sonia, additional, Gelot, Aontoinette, additional, Mignon, Virginie, additional, Favier, Maryline, additional, Brunet, Isabelle, additional, Declèves, Xavier, additional, Tanter, Mickael, additional, Estevez, Raul, additional, Vivien, Denis, additional, Saubaméa, Bruno, additional, and Cohen-Salmon, Martine, additional

Published
2021
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17. Neuropeptide S promotes wakefulness through the inhibition of sleep-promoting VLPO neurons

Author

Chauveau, Frédéric, Claverie, Damien, Lardant, Emma, Varin, Christophe, Hardy, Eléonore, Walter, Augustin, Rouach, Nathalie, Canini, Frédéric, Rancillac, Armelle, Institut de Recherche Biomédicale des Armées (IRBA), Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Neurophysiology [Brussels], Université libre de Bruxelles (ULB), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École du Val de Grâce (EVDG), Service de Santé des Armées, Rancillac, Armelle, École normale supérieure - Paris (ENS-PSL), and Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, GABAergic neurons, VLPO, neurovascular coupling, education, technology, industry, and agriculture, patch-clamp, NPS, feed-forward inhibition, polysomnography, mental disorders, NREM sleep, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], health care economics and organizations, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Nathalie Rouach and Frédéric Canini contributed equally to this work.; International audience; STUDY OBJECTIVES:The regulation of sleep-wake cycles is crucial for the brain's health and cognitive skills. Among the various substances known to control behavioral states, intraventricular injection of neuropeptide S (NPS) has already been shown to promote wakefulness. However, the NPS signaling pathway remains elusive. In this study, we characterized the effects of NPS in the ventrolateral preoptic nucleus (VLPO) of the hypothalamus, one of the major brain structures regulating non-rapid eye movement (NREM) sleep.METHODS:We combined polysomnographic recordings, vascular reactivity, and patch-clamp recordings in mice VLPO to determine the NPS mode of action.RESULTS:We demonstrated that a local infusion of NPS bilaterally into the anterior hypothalamus (which includes the VLPO) significantly increases awakening and specifically decreases NREM sleep. Furthermore, we established that NPS application on acute brain slices induces strong and reversible TTX-sensitive constriction of blood vessels in the VLPO. This effect strongly suggests that the local neuronal network is downregulated in the presence of NPS. At the cellular level, we revealed by electrophysiological recordings and in situ hybridization that NPSR mRNAs are only expressed by non-Gal local GABAergic neurons, which are depolarized by the application of NPS. Simultaneously, we showed that NPS hyperpolarizes sleep-promoting neurons, which is associated with an increased frequency in their spontaneous IPSC inputs.CONCLUSION:Altogether, our data reveal that NPS controls local neuronal activity in the VLPO. Following the depolarization of local GABAergic neurons, NPS indirectly provokes feed-forward inhibition onto sleep-promoting neurons, which translates into a decrease in NREM sleep to favor arousal.

Published
2019

18. In mice and humans, the brain’s blood vessels mature postnatally to acquire barrier and contractile properties

Author

Slaoui, Leila, primary, Gilbert, Alice, additional, Federici, Laetitia, additional, Rancillac, Armelle, additional, Gelot, Antoinette, additional, Favier, Maryline, additional, Robil, Noémie, additional, Letort, Gaëlle, additional, Dias, Karine, additional, Jourdren, Laurent, additional, Mailly, Philippe, additional, Auvity, Sylvain, additional, Cisternino, Salvatore, additional, Cohen-Salmon, Martine, additional, and Boulay, Anne-Cécile, additional

Published
2021
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21. FENS-2020-e-Poster: Sleep- and wake-dependent changes of sleep-promoting neurons within the VLPO

Author

Walter, Augustin, Rancillac, Armelle, Pezet, Sophie, Chauveau, Frédéric, Canini, Frédéric, and Rouach, Nathalie

Abstract

Sleep is one of the most universal and essential biological process of life. Yet sleep disorders still affect up to one-third of the population and are associated with a wide range of deleterious health consequences including an increased risk of hypertension, diabetes, obesity, depression and stroke. Thus it is essential to investigate the neurobiological processes underlying sleep physiology. We here focused on a key sleep-promoting component of brain circuitry: the ventrolateral preoptic nucleus (VLPO). Aim: We characterized the circadian morpho- physiological changes occurring in the VLPO to identify new key mechanisms involved in slow-waves sleep regulation.

Published
2020
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22. Neuropeptide S promotes wakefulness through the inhibition of sleep-promoting ventrolateral preoptic nucleus neurons

Author

Chauveau, Frédéric, Claverie, Damien, Lardant, Emma, Varin, Christophe, Hardy, Eléonore, Walter, Augustin, Canini, Frédéric, Rouach, Nathalie, Rancillac, Armelle, Chauveau, Frédéric, Claverie, Damien, Lardant, Emma, Varin, Christophe, Hardy, Eléonore, Walter, Augustin, Canini, Frédéric, Rouach, Nathalie, and Rancillac, Armelle

Abstract

STUDY OBJECTIVES: The regulation of sleep-wake cycles is crucial for the brain's health and cognitive skills. Among the various substances known to control behavioral states, intraventricular injection of neuropeptide S (NPS) has already been shown to promote wakefulness. However, the NPS signaling pathway remains elusive. In this study, we characterized the effects of NPS in the ventrolateral preoptic nucleus (VLPO) of the hypothalamus, one of the major brain structures regulating non-rapid eye movement (NREM) sleep. METHODS: We combined polysomnographic recordings, vascular reactivity, and patch-clamp recordings in mice VLPO to determine the NPS mode of action. RESULTS: We demonstrated that a local infusion of NPS bilaterally into the anterior hypothalamus (which includes the VLPO) significantly increases awakening and specifically decreases NREM sleep. Furthermore, we established that NPS application on acute brain slices induces strong and reversible tetrodotoxin (TTX)-sensitive constriction of blood vessels in the VLPO. This effect strongly suggests that the local neuronal network is downregulated in the presence of NPS. At the cellular level, we revealed by electrophysiological recordings and in situ hybridization that NPSR mRNAs are only expressed by non-Gal local GABAergic neurons, which are depolarized by the application of NPS. Simultaneously, we showed that NPS hyperpolarizes sleep-promoting neurons, which is associated with an increased frequency in their spontaneous IPSC inputs. CONCLUSION: Altogether, our data reveal that NPS controls local neuronal activity in the VLPO. Following the depolarization of local GABAergic neurons, NPS indirectly provokes feed-forward inhibition onto sleep-promoting neurons, which translates into a decrease in NREM sleep to favor arousal., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2020

27. AlicE 2630: Experimento humano

Author

Rancillac, Armelle and Rancillac, Armelle

Subjects
Future, The--Fiction, Life--Fiction, Human beings--Fiction
Abstract

A sus 31 años, AlicE lleva una vida festiva y trabaja en el Centro Universal de Planificación, el CUP. Desde la fusión de naciones en 2258, este organismo define el plan de vida de cada persona en un mundo sin clases sociales ni dinero. Esta avanzada civilización adopta nuevas reglas para poder formar una familia e incluso para sus costumbres sexuales alternativas. ¿Pero todo este desarrollo tecnológico no se ha entrometido ya demasiado en nuestras vidas? ¿No podría existir otra realidad? En toda la Historia, la humanidad ha explorado lo invisible para intentar comprender su mundo. Esta se pregunta sobre lo que ocultan las apariencias e intenta mejorar su calidad de vida. ¿Acaso todo el progreso exponencial del conocimiento no está atentando contra nuestra verdadera naturaleza? ¿No seguimos siendo, en el fondo, unos simples cazadores prehistóricos?

Published
2018

28. Alice 2630_Experiência Humana

Author

Rancillac, Armelle, TotalBoox, TBX, Rancillac, Armelle, TotalBoox, and TBX

Subjects
Future, The--Fiction, Human beings--Fiction, Life--Fiction
Abstract

Descrição do livro: Aos 31 anos, AlicE leva uma vida alegre e trabalha no centro universal de planeamento, o CUP. Desde a unificação das nações, em 2258, este organismo define o plano de vida de cada pessoa, num mundo sem classes sociais nem moeda. Esta civilização, altamente avançada, face aos padrões tradicionais, adota novos códigos de família e estilos de vida sexual alternativos. Mas este incrível desenvolvimento tecnológico não se terá imiscuído demasiado nas nossas vidas? Não poderia existir uma outra realidade? Desde sempre, o homem tem explorado o insondável na tentativa de compreender o mundo, questionando-se sobre o que está por detrás das aparências, tentando melhorar as suas condições de vida. Mas não estará o progresso exponencial do nosso conhecimento desfasado da nossa natureza interior? Basicamente, não continuaremos nós a ser caçadores pré-históricos?

Published
2016

29. Les astrocytes du VLPO, acteurs clés de la régulation du sommeil

Author

Walter, Augustin and Rancillac, Armelle

Abstract

Les astrocytes sont des cellules gliales du système nerveux central, dont le rôle dans la physiologie cérébrale est de mieux en mieux compris. Récemment, leur implication dans la régulation du sommeil, notamment via la régulation des taux extracellulaires d’adénosine, a été particulièrement étudiée. Cette purine joue en effet un rôle central dans la régulation du sommeil, en particulier au niveau du noyau préoptique ventrolatéral (VLPO), une région clé du cerveau impliquée dans la promotion du sommeil lent. En combinant des quantifications enzymatiques in situ des taux d’adénosine, des mesures en vidéomicroscopie infrarouge de la réactivité vasculaire, ainsi que des enregistrements en patch-clamp de l’activité neuronale, il a été démontré que ces astrocytes du VLPO ajustent les taux d’adénosine extracellulaires en réponse du glucose disponible, des taux de prostaglandine D2(PGD2) et du moment circadien. L’adénosine extracellulaire active les récepteurs A2Asitués au niveau des cellules endothéliales des vaisseaux sanguins et des neurones promoteurs du sommeil du VLPO, afin d’ajuster simultanément l’augmentation du flux sanguin à l’activité neuronale. Ainsi, l’apport énergétique nécessaire à l’activation neuronale est assuré, ce qui permet ainsi une activation optimale du VLPO et le sommeil. Ce mécanisme gliocentrique est également intégratif, adaptant la réponse adénosinergique au moment circadien, plus important quand la pression de sommeil est élevée, que lorsqu’elle est faible. Les astrocytes du VLPO ne se contentent donc pas de soutenir structurellement les neurones, mais jouent également un rôle dynamique et intégratif dans la régulation des états de vigilance.

Published
2024
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34. Glucose Induces Slow-Wave Sleep by Exciting the Sleep-Promoting Neurons in the Ventrolateral Preoptic Nucleus: A New Link between Sleep and Metabolism.

Author

Varin, Christophe, Rancillac, Armelle, Geoffroy, Hélène, Arthaud, Sebastien, Fort, Patrice, Gallopin, Thierry, Varin, Christophe, Rancillac, Armelle, Geoffroy, Hélène, Arthaud, Sebastien, Fort, Patrice, and Gallopin, Thierry

Abstract

Sleep-active neurons located in the ventrolateral preoptic nucleus (VLPO) play a crucial role in the induction and maintenance of slow-wave sleep (SWS). However, the cellular and molecular mechanisms responsible for their activation at sleep onset remain poorly understood. Here, we test the hypothesis that a rise in extracellular glucose concentration in the VLPO can promote sleep by increasing the activity of sleep-promoting VLPO neurons. We find that infusion of a glucose concentration into the VLPO of mice promotes SWS and increases the density of c-Fos-labeled neurons selectively in the VLPO. Moreover, we show in patch-clamp recordings from brain slices that VLPO neurons exhibiting properties of sleep-promoting neurons are selectively excited by glucose within physiological range. This glucose-induced excitation implies the catabolism of glucose, leading to a closure of ATP-sensitive potassium (KATP) channels. The extracellular glucose concentration monitors the gating of KATP channels of sleep-promoting neurons, highlighting that these neurons can adapt their excitability according to the extracellular energy status. Together, these results provide evidence that glucose may participate in the mechanisms of SWS promotion and/or consolidation., info:eu-repo/semantics/published

Published
2015

35. Bidirectional synaptic plasticity and motor learning in the cerebellum

Author

Rancillac, Armelle, Crépel, Francis, Rancillac, Armelle, Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-École normale supérieure - Paris (ENS Paris), and Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

36. Nitric Oxide Release during Evoked Neuronal Activity In Cerebellum Slices. Detection with Platinized Carbon Fiber Microelectrodes

Author

Amatore, Christian, Arbault, Stéphane, Cauli, Bruno, Guille, Manon, Rancillac, Armelle, Rossier, Jean, Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Neurobiologie et diversité cellulaire (NDC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL)

Subjects
[CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

37. BDNF-Dependent Plasticity Induced by Peripheral Inflammation in the Primary Sensory and the Cingulate Cortex Triggers Cold Allodynia and Reveals a Major Role for Endogenous BDNF As a Tuner of the Affective Aspect of Pain

Author

Thibault, Karine, primary, Lin, Wee Khang, additional, Rancillac, Armelle, additional, Fan, Marie, additional, Snollaerts, Thibaut, additional, Sordoillet, Vallier, additional, Hamon, Michel, additional, Smith, George M., additional, Lenkei, Zsolt, additional, and Pezet, Sophie, additional

Published
2014
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47. Characterisation of type I and type II nNOS-expressing interneurons in the barrel 2 cortex of mouse.

Author

Perrenoud, Quentin, Geoffroy, Hélène, Gauthier, Benjamin, Rancillac, Armelle, Alfonsi, Fabienne, Kessaris, Nicoletta, Rossier, Jean, Vitalis, Tania, and Gallopin, Thierry

Subjects
NITRIC-oxide synthases, SOMATOSTATIN, PARVALBUMINS, GLUTAMATE decarboxylase, VASOACTIVE intestinal peptide, IMMUNOHISTOCHEMISTRY
Abstract

In the neocortex, neuronal Nitric Oxide-Synthase (nNOS) is essentially expressed in two classes of GABAergic neurons: type I neurons displaying high levels of expression and type II neurons displaying weaker expression. Using immunocytochemistry in mice expressing GFP under the control of the glutamic acid decarboxylase 67k (GAD67) promoter, we studied the distribution of type I and type II neurons in the barrel cortex and their expression of parvalbumin (PV), somatostatin (SOM) and vasoactive intestinal peptide (VIP). We found that type I neurons were predominantly located in deeper layers and expressed SOM (91.5%) while type II neurons were concentrated in layer II/III and VI and expressed PV (17.7%), SOM (18.7%) and VIP (10.2%). We then characterised neurons expressing nNOS mRNA (n = 42 cells) ex vivo, using whole-cell recordings coupled to single-cell RT-PCR and biocytin labelling. Unsupervised cluster analysis of this sample disclosed four classes. One cluster (n=7) corresponded to large, deep layer neurons, displaying a high expression of SOM (85.7%) and was thus very likely to correspond to type I neurons. The three other clusters were identified as putative type II cells and corresponded to neurogliaform-like interneurons (n=19), deep layer neurons expressing PV or SOM (n=9) and neurons expressing VIP (n=7). Finally, we performed nNOS immunohistochemistry on mouse lines in which GFP labelling revealed the expression of two specific developmental genes (Lhx6 and 5-HT3A). We found that type I neurons expressed Lhx6 but never 5-HT3A, indicating that they originate in the medial ganglionic eminence (MGE). Type II neurons expressed Lhx6 (63%) and 5-HT3A (34.4%) supporting their derivation either from the MGE or from the caudal ganglionic eminence (CGE) and the entopeduncular preoptic area (AEP/PO). Together, our results in the barrel cortex of mouse support the view that type I neurons form a specific class of SOMexpressing neurons while type II neurons comprise at least three classes. [ABSTRACT FROM AUTHOR]

Published
2012
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48. Glutamatergic Control of Microvascular Tone by Distinct GABA Neurons in the Cerebellum.

Author

Rancillac, Armelle, Rossier, Jean, Guille, Manon, Xin-Kang Tong, Geoffroy, Hélène, Amatore, Christian, Arbault, Stéphane, Hamel, Edith, and Cauli, Bruno

Subjects
*KUPFFER cells, *PURKINJE cells, *GABA, *AMINO acid neurotransmitters, *NEURONS, *CEREBELLUM
Abstract

The tight coupling between increased neuronal activity and local cerebral blood flow, known as functional hyperemia, is essential for normal brain function. However, its cellular and molecular mechanisms remain poorly understood. In the cerebellum, functional hyperemia depends almost exclusively on nitric oxide (NO). Here, we investigated the role of different neuronal populations in the control of microvascular tone by in situ amperometric detection of NO and infrared videomicroscopy of microvessel movements in rat cerebellar slices. Bath application of an NO donor induced both NO flux and vasodilation. Surprisingly, endogenous release of NO elicited by glutamate was accompanied by vasoconstriction that was abolished by inhibition of Ca2+-phopholipase A2 and impaired by cyclooxygenase and thromboxane synthase inhibition and endothelin A receptor blockade, indicating a role for prostanoids and endothelin 1 in this response. Interestingly, direct stimulation of single endothelin 1-immunopositive Purkinje cells elicited constriction of neighboring microvessels. In contrast to glutamate, NMDA induced both NO flux and vasodilation that were abolished by treatment with a NO synthase inhibitor or with tetrodotoxin. These findings indicate that NO derived from neuronal origin is necessary for vasodilation induced by NMDA and, furthermore, that NO-producing interneurons mediate this vasomotor response. Correspondingly, electrophysiological stimulation of single nitrergic stellate cells by patch clamp was sufficient to release NO and dilate both intraparenchymal and upstream pial microvessels. These findings demonstrate that cerebellar stellate and Purkinje cells dilate and constrict, respectively, neighboring microvessels and highlight distinct roles for different neurons in neurovascular coupling. [ABSTRACT FROM AUTHOR]

Published
2006
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50. Multiparametric characterization of neuronal subpopulations in the ventrolateral preoptic nucleus

Author

Aude Sangare, Romain Dubourget, Hélène Geoffroy, Armelle Rancillac, Thierry Gallopin, Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by a Grant from ANR-12-JSV4-001-01, the Centre National de la Recherche Scientifique (CNRS), the French Institute of Health and Medical Research (Inserm) and ESPCI ParisTech., ANR-12-JSV4-0001,Metabosleep,Les neurones promoteurs du sommeil du VLPO intègrent et modulent l'apport énergétique local(2012), Rancillac, Armelle, Jeunes Chercheuses et Jeunes Chercheurs - Les neurones promoteurs du sommeil du VLPO intègrent et modulent l'apport énergétique local - - Metabosleep2012 - ANR-12-JSV4-0001 - JC - VALID, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), and Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, 0301 basic medicine, Patch-Clamp Techniques, Nerve net, VLPO, [SDV]Life Sciences [q-bio], Action Potentials, Mice, Norepinephrine, chemistry.chemical_compound, Ward’s clustering, 0302 clinical medicine, Cluster Analysis, Neurotransmitter, Neurons, Ward's clustering, General Neuroscience, Sleep-promoting neuron, Synaptic Potentials, Preoptic area, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Unsupervised clustering of VLPO neurons Sleep-promoting neuron, Anatomy, Serotonin, Histology, Models, Neurological, Biophysics, In Vitro Techniques, Biology, Inhibitory postsynaptic potential, Statistics, Nonparametric, 03 medical and health sciences, Low-threshold calcium spike (LTS), Biocytin, Biological neural network, medicine, Animals, Patch clamp, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Preoptic Area, Electric Stimulation, Mice, Inbred C57BL, Electrophysiology, 030104 developmental biology, Animals, Newborn, chemistry, nervous system, noradrenaline, Nerve Net, Neuroscience, 030217 neurology & neurosurgery
Abstract

International audience; The characterization of neuronal properties is a necessary first step toward understanding how the ventrolateral preoptic nucleus (VLPO) neuronal network regulates slow-wave sleep (SWS). Indeed, the electrophysiological heterogeneity of VLPO neurons suggests the existence of subtypes that could differently contribute in SWS induction and maintenance. The aim of the present study was to define cell classes in the VLPO using an unsupervised clustering classification method. Electrophysiological features extracted from 289 neurons recorded in whole-cell patch-clamp allowed the identification of three main classes of VLPO neurons subdivided into five distinct subpopulations (cluster 1, 2a, 2b, 3a and 3b). The high occurrence of a low-threshold calcium spike (LTS) was one of the most distinctive features of cluster 1 and 3. Since sleep-promoting neurons are generally identified by their ability to generate an LTS and by their inhibitory response to noradrenaline (NA), 189 neurons from our dataset were also tested for this neurotransmitter. Neurons from cluster 3 were the most frequently inhibited by NA. Biocytin labeling and Neurolucida reconstructions of 112 neurons furthermore revealed a small dendritic arbor of cluster 3b neurons compared, in particular, to cluster 2b neurons. Altogether, we performed an exhaustive characterization of VLPO neuronal subtypes that is a crucial step toward a better understanding of the neuronal network within the VLPO and thereby sleep physiology.

Published
2017

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