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29 results on '"Ranganathan, Sampathkumar"'

16. Cloning, functional characterization and screening of potential inhibitors for Chilo partellus chitin synthase A using in silico, in vitro and in vivo approaches

Author

Ranganathan, Sampathkumar, Ilavarasi, Anbumani Velmurugan, Palaka, Bhagath Kumar, Kuppusamy, Dheebika, and Ampasala, Dinakara Rao

Subjects
integumentary system, fungi, skin and connective tissue diseases
Abstract

Chitin synthase (CHS) is one of the crucial enzymes that play an essential role in chitin synthesis during the molting process, and it is considered to be the specific target to control insect pests. Currently, there are no potent inhibitors available in the market, which specifically target this enzyme. Pyrimidine nucleoside peptide, nikkomycin Z, binds to nucleotide-binding sites of fungal and insect CHS. But, their mode of action is still fragmentary due to the lack of a 3Dstructure of CHS. Chilo partellus is a severe pest insect of major food crops such as maize and sorghum, in an attempt to target integument expressed cuticular CpCHS. The CpChsA cDNA was cloned, and subsequently, their developmental and tissue-specific expression was studied. The 3D structure of the CHS catalytic domain was modeled, after which natural compounds were screened using a virtual screening workflow and resulted in the identification of five hit molecules. Molecular dynamics simulations were performed to investigate the dynamics and interactions of hits with CpCHS. The obtained results revealed that the compounds kasugamycin, rutin and robinin could act as potent inhibitors of CpCHS. All three molecules were observed to significantly reduce the chitin production as validated using in vitro and in vivo studies. Thus, this study aims to provide a set of novel inhibitor molecules against CpCHS for controlling the pest population. Communicated by Ramaswamy H. Sarma

Published
2020
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20. Corrigendum to: Inhibition of Microbial Quorum Sensing Mediated Virulence Factors by Pestalotiopsis sydowian

Author

Parasuraman, Paramanantham, primary, Devadatha, B, additional, Sarma, V. Venkateswara, additional, Ranganathan, Sampathkumar, additional, Ampasala, Dinakara Rao, additional, Reddy, Dhanasekhar, additional, Kumavath, Ranjith N., additional, Patel, Sanjay K. S., additional, Kalia, Vipin Chandra, additional, Lee, Jung-Kul, additional, and Siddhardha, Busi, additional

Published
2020
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23. Cloning, functional characterization and screening of potential inhibitors for Chilo partelluschitin synthase A using in silico, in vitroand in vivoapproaches

Author

Ranganathan, Sampathkumar, Ilavarasi, Anbumani Velmurugan, Palaka, Bhagath Kumar, Kuppusamy, Dheebika, and Ampasala, Dinakara Rao

Abstract

AbstractChitin synthase (CHS) is one of the crucial enzymes that play an essential role in chitin synthesis during the molting process, and it is considered to be the specific target to control insect pests. Currently, there are no potent inhibitors available in the market, which specifically target this enzyme. Pyrimidine nucleoside peptide, nikkomycin Z, binds to nucleotide-binding sites of fungal and insect CHS. But, their mode of action is still fragmentary due to the lack of a 3Dstructure of CHS. Chilo partellusis a severe pest insect of major food crops such as maize and sorghum, in an attempt to target integument expressed cuticular CpCHS. The CpChsAcDNA was cloned, and subsequently, their developmental and tissue-specific expression was studied. The 3D structure of the CHS catalytic domain was modeled, after which natural compounds were screened using a virtual screening workflow and resulted in the identification of five hit molecules. Molecular dynamics simulations were performed to investigate the dynamics and interactions of hits with CpCHS. The obtained results revealed that the compounds kasugamycin, rutin and robinin could act as potent inhibitors of CpCHS. All three molecules were observed to significantly reduce the chitin production as validated using in vitroand in vivostudies. Thus, this study aims to provide a set of novel inhibitor molecules against CpCHS for controlling the pest population. Communicated by Ramaswamy H. Sarma

Published
2022
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27. Leo satellite network control and operation software architecture based on multi-agent system

Author

Ranganathan Sampathkumar, Tan Soon Hie, and School of Electrical and Electronic Engineering

Subjects
Engineering::Electrical and electronic engineering::Satellite telecommunication [DRNTU]
Abstract

103 p. The capability and performance of a low earth orbit satellite can be enhanced and augmented with an efficient ground station gateway network management system. Primary requirements for such a distributed network management system can be better techniques for managing operations, security, auditing, data processing, reconfiguration and fault tolerance. ​Master of Science (Communications Engineering)

Published
2006

28. The Effects of Symbol Product Relevance and Religiosity on Consumer Perceptions of Christian Symbols in Advertising

Author

Henley, WalterHodges, Philhours, Melodie, Ranganathan, SampathKumar, and Bush, AlanJ.

Abstract

AbstractThis research supports and extends the theoretical foundation of relevant associative linkages via examination of the effects of Christian cues or symbols on relevant and irrelevant symbol product ad evaluations. Results indicate that a relevant Christian symbol product linkage supports the basic premises of associative linkage theory with significant positive ad evaluative efficacy (Aad, Ab, and PI) while an irrelevant Christian symbol product linkage has a significant negative impact on ad evaluative efficacy. In addition, the study also indicates that religiosity and gender of the respondent has a significant moderating impact on the evaluation of an ad that has a Christian symbol under relevant symbol product linkage conditions. Moreover, and possibly most significantly, the interaction effect between relevancy and religiosity indicates that this interplay combines to significantly affect the diagnostic efficacy of the ad for relevant symbol product linkages. Overall, results indicate the need for practitioners to carefully choose a relevant context when co-opting Christian symbols for their advertisements and to avoid indiscriminate use of Christian symbols in ads while also considering the religiosity of the target audience.

Published
2009
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29. In silico Binding Profile Analysis and In vitro Investigation on Chitin Synthase Substrate and Inhibitors from Maize Stem Borer, Chilo partellus .

Author

Ranganathan S, Ampasala DR, Palaka BK, Ilavarasi AV, Patidar I, Poovadan LP, and Sapam TD

Subjects
Chitin, Fungi, Molecular Docking Simulation, Chitin Synthase, Zea mays
Abstract

Introduction: Insect growth and metamorphosis are strictly dependent on the structural changes that occur in chitin containing tissues and organs. Chitin synthase catalyzes chitin polymerization by β-(1, 4) glycosidic linkage of N-acetyl-D-glucosamine (GlcNAc) monomers; the major component of insect cuticles. Targeting this enzyme could be a promising strategy to control insect pests while avoiding adverse effects on coexisting populations. Nikkomycin Z and polyoxins are commercially available fungal inhibitors known to bind to the nucleotide-binding sites of insects and fungal chitin synthase. But the binding mode of chitin synthase has not been explored to date as its structure is not available yet., Methods: To understand the structural features of the Chilo partellus chitin synthase enzyme (CpCHS), the three-dimensional (3D) structure of the CpCHS catalytic domain was modeled using ROBETTA webserver. The obtained model was used to investigate the binding mode of its substrate, uridine diphosphate-N-acetyl-D-glucosamine (UDP-GlcNAc), and inhibitors (nikkomycin Z and polyoxins) by molecular docking approach using Schrödinger Suite-Maestro v9.2. The docked complexes were further investigated for their interaction stability by performing molecular dynamics (MD) simulations using GROMACS v5.1.2., Results: Our study highlighted the significance of various interactions made by CHS residues present in the Walker-B loop and donor-binding motifs with the substrate (UDP-GlcNAc), and GEDR motif with an acceptor (GlcNAc). Also, the interactions of the QRRRW motif while forming chitin polymer were explored. We observed that the inhibitors exhibited good binding affinity with these motifs, indicated by their docking and binding affinity scores., Conclusion: In vitro analysis suggested that nikkomycin Z showed higher inhibition of chitin synthase activity at a concentration of 2.5 μg.L -1 . Our study provided insights into the crucial interactions of chitin synthase while designing inhibitors against insect pests., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Published
2021
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