235 results on '"Rath SK"'
Search Results
2. Interpreting COVID-19 outcomes in pregnancy needs knowledge of prevalent conditions in that time frame
- Author
-
Mohini M, Sakir Ahmed, and Rath Sk
- Subjects
medicine.medical_specialty ,Pregnancy ,2019-20 coronavirus outbreak ,Time frame ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,medicine ,Obstetrics and Gynecology ,Intensive care medicine ,medicine.disease ,business - Published
- 2022
3. Detecting Product Review Spammers Using Principles of Big Data
- Author
-
Rout, JK, Dalmia, A, Rath, SK, Mohanta, BK, Ramasubbareddy, S, and H. Gandomi, A
- Subjects
Business & Management ,08 Information and Computing Sciences, 09 Engineering, 15 Commerce, Management, Tourism and Services - Published
- 2021
4. Surgical approach for polycystic ovarian syndrome in management of infertility
- Author
-
Rath, SK, Sharma, RK, and Duggal, BS
- Published
- 2006
- Full Text
- View/download PDF
5. Vagina
- Author
-
Pandit, Suchitra, primary, Trivedi, SS, additional, Batra, Swaraj, additional, Dhaliwal, Lakhbir, additional, Singh, S, additional, Sarkar, Barun, additional, Urala, MS, additional, Chandra, Phool, additional, Rath, SK, additional, and Jasleen, Jasleen, additional
- Published
- 2009
- Full Text
- View/download PDF
6. Effect of cryopreservation on semen sample
- Author
-
Rath, SK, Tarneja, P, and Singh, S Man
- Published
- 2004
- Full Text
- View/download PDF
7. Laparoscopic management of adnexal masses
- Author
-
Duggal, BS, Tarneja, P, Sharma, RK, Rath, SK, and Wadhwa, RD
- Published
- 2004
- Full Text
- View/download PDF
8. Laparoscopic management of ectopic pregnancies
- Author
-
Duggal, BS, Tarneja, P, Sharma, RK, Rath, SK, and Wadhwa, RD
- Published
- 2004
- Full Text
- View/download PDF
9. Fertility enhancing hysteroscopic surgery: Our experience
- Author
-
Duggal, BS, Sharma, RK, Tarneja, P, Rath, SK, and Wadhwa, RD
- Published
- 2003
- Full Text
- View/download PDF
10. Office hysteroscopy: An insight
- Author
-
Duggal, BS, Sandeep, Maj, Tarneja, P, Wadhwa, RD, and Rath, SK
- Published
- 2002
- Full Text
- View/download PDF
11. Military environment and fecundity: A study of women in uniform
- Author
-
Rath, SK, Tarneja, P, Duggal, BS, Chawla, Ashima, and Tugnait, P
- Published
- 2002
- Full Text
- View/download PDF
12. Ovarian hyperstimulation syndrome during induction of ovulation for intra uterine insemination
- Author
-
Rath, SK, Sharma, RK, Tarneja, P, Chattopadhyay, AB, and Wadhwa, RD
- Published
- 2001
- Full Text
- View/download PDF
13. COSMETIC SCAR AND SCOPE OF REVERSAL AFTER PUERPERAL STERILISATION
- Author
-
RATH, SK, CHATTOPADHYAYA, AB, and TARNEJA, P
- Published
- 2000
- Full Text
- View/download PDF
14. Dental Management for Patients in ICU
- Author
-
Rath, SK, primary and Kumar, U, additional
- Published
- 2016
- Full Text
- View/download PDF
15. Oral lichenoid contact reaction to a complete denture: A rare case report
- Author
-
Arnav, Mukherji, primary and Rath, SK, additional
- Published
- 2016
- Full Text
- View/download PDF
16. Evaluation of serum ceruloplasmin in aggressive and chronic periodontitis patients
- Author
-
Harshavardhana, B, primary, Mukherjee, Manish, additional, and Rath, SK, additional
- Published
- 2013
- Full Text
- View/download PDF
17. Gingival cyst of adult: A rare case
- Author
-
Malali, VijayalaxmiV, primary, Satisha, TS, additional, Jha, AK, additional, and Rath, SK, additional
- Published
- 2012
- Full Text
- View/download PDF
18. Cationic electron-beam curing of a high-functionality epoxy: effect of post-curing on glass transition and conversion
- Author
-
Rath, SK, primary, Boey, FYC, additional, and Abadie, MJM, additional
- Published
- 2004
- Full Text
- View/download PDF
19. Multifetal Pregnancy Reduction
- Author
-
Rath, SK, primary, Kumar, Sushil, additional, Sharma, RK, additional, and Rao, PS, additional
- Published
- 2004
- Full Text
- View/download PDF
20. Military Environment nad Fecundity: A Study of Women in Uniform
- Author
-
Rath, SK, primary
- Published
- 2003
- Full Text
- View/download PDF
21. ABNORMÀL CYTOLOGY REPORT AND FURTHER MANAGEMENT OF PRECANCEROUS LESION: EFFECT OF CHANGE TO TBS
- Author
-
RATH, SK, primary, TARNEJA, P, additional, and CHATTOPADYAYA, AB, additional
- Published
- 2001
- Full Text
- View/download PDF
22. UNDERSTANDING MALE FACTOR IN INFERTILITY
- Author
-
CHATTOPADHYAY, AB, primary and RATH, SK, additional
- Published
- 1999
- Full Text
- View/download PDF
23. PII: S0377-1237(03)80133-8
- Author
-
Rath, SK
- Published
- 2003
- Full Text
- View/download PDF
24. Letter to the editor.
- Author
-
Rath, SK
- Published
- 2003
- Full Text
- View/download PDF
25. VEGF-A immunohistochemical and mRNA expression in tissues and its serum levels in potentially malignant oral lesions and oral squamous cell carcinomas.
- Author
-
Nayak S, Goel MM, Chandra S, Bhatia V, Mehrotra D, Kumar S, Makker A, Rath SK, and Agarwal SP
- Abstract
The aim of the study was to investigate whether the estimation of circulating Vascular endothelial growth factor-A (VEGF-A) levels by ELISA could be used as surrogate of VEGF-A expression in tissues of pre-malignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC) as compared to that in healthy controls. The study samples comprised of tissue and blood samples from 60 PMOLs, 60 OSCC, and 20 healthy controls. Serum VEGF-A levels were determined by an ELISA based assay (Quantikine human VEGF; R & D System, Minneapolis USA). Tissue VEGF-A expression and microvessel density (MVD) were assessed by immunohistochemistry (IHC) using antibodies against VEGF-A and CD-34 on formalin fixed paraffin embedded (FFPE) tissue sections. VEGF-A mRNA expression was analyzed by real-time PCR in snap frozen tissues. Serum VEGF-A levels and immunohistochemical VEGF-A expression were significantly high in PMOLs and OSCC in comparison with controls. VEGF mRNA gene expression showed more than 50-fold increase in PMOLs and OSCC. VEGF-A levels in serum correlated in a linear fashion with the tissue expression in oral pre-malignant and malignant lesions, suggesting that the serum levels may serve as surrogate material for tissue expression of VEGF-A. [ABSTRACT FROM AUTHOR]
- Published
- 2012
26. Effectiveness of autologous leukocyte-platelet-rich fibrin on the rate of maxillary canine retraction, rotation, pain, and soft tissue healing: A split-mouth randomized controlled trial.
- Author
-
Satapathy SK, Das SK, Barik AK, Mohanty D, Rath SK, and Mishra M
- Abstract
Objective: To assess the effectiveness of leukocyte-platelet-rich fibrin (L-PRF) compared with conventional treatment on canine retraction, rotation, pain, and soft tissue healing., Methods: Sixteen adult patients aged 18-25 years (10 females, and 6 males; mean age 22.25 ± 2.26 years) with Class I bimaxillary protrusion and Class II div 1 malocclusion participated in this single-center, split-mouth randomized controlled trial at the Orthodontics Department of a single hospital in SCB Dental College and Hospital, Cuttack, India. Randomization was performed using a computer-assisted function with a 1:1 allocation ratio. The intervention included the placement of L-PRF on the experimental side and follow-up for 90 days. The primary outcome measures were canine retraction, rotation, pain, and soft tissue healing. The range of tooth movement was evaluated at 15-day intervals: 0th day (T0), 15th day (T1), 30th day (T2), 45th day (T3), 60th day (T4), 75th day (T5), and 90th day (T6). Canine rotation was assessed at T0 and T6, and pain and soft tissue healing were evaluated on the 3rd, 7th, and 15th days of the treatment., Results: Cumulatively, the L-PRF group demonstrated a significantly greater tooth movement as compared to conventional treatment group ( P < 0.001). Overall, canine retraction was 1.5 times greater on the L-PRF side than on the control side. Canine rotation showed no significant relationship, whereas pain and soft tissue healing were significantly better on the L-PRF side than on the control side., Conclusions: Local administration of L-PRF amplifies canine retraction while improving pain and soft tissue repair.
- Published
- 2024
- Full Text
- View/download PDF
27. Evaluation of dentoalveolar changes following maxillary incisor intrusion with one vs two anterior miniscrews in subjects with gummy smile: a randomized clinical trial.
- Author
-
M M, Das SK, Barik AK, Raj SC, Mishra M, Rath SK, and Sah S
- Subjects
- Humans, Female, Male, Adult, Alveolar Process diagnostic imaging, Alveolar Process pathology, Young Adult, Overbite therapy, Root Resorption diagnostic imaging, Root Resorption etiology, Tooth Movement Techniques methods, Tooth Movement Techniques instrumentation, Incisor diagnostic imaging, Smiling, Bone Screws, Maxilla diagnostic imaging, Cone-Beam Computed Tomography methods, Orthodontic Anchorage Procedures instrumentation, Orthodontic Anchorage Procedures methods
- Abstract
Objectives: To examine dentoalveolar changes following intrusion of maxillary incisors with one or two anterior miniscrews in subjects with gummy smile and deep bite., Materials and Methods: Forty-three subjects were selected and divided into two groups: group I (22 subjects: 15 women, 7 men; mean age 30 ± 10 years) received one miniscrew between the upper central incisors, and group II (21 subjects: 16 women, 5 men; mean age 30 ± 10 years) received two miniscrews between the canines and lateral incisors. Dentoalveolar parameters, including amount of intrusion, root resorption, incisor inclination, alveolar bone thickness, and buccal alveolar crest height (cementoenamel junction to labial alveolar crest), were evaluated using cone-beam computed tomography scans obtained before and after intrusion. The intergroup comparison was analyzed using a paired t-test and unpaired t-test to determine significant changes within and between groups., Results: The amount of intrusion was significantly greater in group II than in group I (P < .05). No statistically significant differences were found between groups I and II for changes in incisor inclination, labial bone thickness, and buccal alveolar crest height (P > .05)., Conclusions: Maxillary central and lateral incisor intrusion was significantly greater in subjects treated with two miniscrews. Root resorption of the maxillary central incisors was notably greater in subjects with one miniscrew, while maxillary lateral incisor resorption was greater in subjects treated with two miniscrews., (© 2024 by The EH Angle Education and Research Foundation, Inc.)
- Published
- 2024
- Full Text
- View/download PDF
28. Loss of tet methyl cytosine dioxygenase 3 (TET3) enhances cardiac fibrosis via modulating the DNA damage repair response.
- Author
-
Rath SK, Nyamsuren G, Tampe B, Yu DS, Hulshoff MS, Schlösser D, Maamari S, Zeisberg M, and Zeisberg EM
- Subjects
- Animals, Mice, Humans, DNA Damage drug effects, Cell Proliferation drug effects, Cell Proliferation genetics, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, DNA Repair drug effects, Myocardium pathology, Myocardium metabolism, Male, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins metabolism, Fibrosis genetics, Dioxygenases genetics, Dioxygenases metabolism, Fibroblasts drug effects, Fibroblasts metabolism
- Abstract
Background: Cardiac fibrosis is the hallmark of all forms of chronic heart disease. Activation and proliferation of cardiac fibroblasts are the prime mediators of cardiac fibrosis. Existing studies show that ROS and inflammatory cytokines produced during fibrosis not only signal proliferative stimuli but also contribute to DNA damage. Therefore, as a prerequisite to maintain sustained proliferation in fibroblasts, activation of distinct DNA repair mechanism is essential., Result: In this study, we report that TET3, a DNA demethylating enzyme, which has been shown to be reduced in cardiac fibrosis and to exert antifibrotic effects does so not only through its demethylating activity but also through maintaining genomic integrity by facilitating error-free homologous recombination (HR) repair of DNA damage. Using both in vitro and in vivo models of cardiac fibrosis as well as data from human heart tissue, we demonstrate that the loss of TET3 in cardiac fibroblasts leads to spontaneous DNA damage and in the presence of TGF-β to a shift from HR to the fast but more error-prone non-homologous end joining repair pathway. This shift contributes to increased fibroblast proliferation in a fibrotic environment. In vitro experiments showed TET3's recruitment to H2O2-induced DNA double-strand breaks (DSBs) in mouse cardiac fibroblasts, promoting HR repair. Overexpressing TET3 counteracted TGF-β-induced fibroblast proliferation and restored HR repair efficiency. Extending these findings to human cardiac fibrosis, we confirmed TET3 expression loss in fibrotic hearts and identified a negative correlation between TET3 levels, fibrosis markers, and DNA repair pathway alteration., Conclusion: Collectively, our findings demonstrate TET3's pivotal role in modulating DDR and fibroblast proliferation in cardiac fibrosis and further highlight TET3 as a potential therapeutic target., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
29. Oral Administration of a Specific p300/CBP Lysine Acetyltransferase Activator Induces Synaptic Plasticity and Repairs Spinal Cord Injury.
- Author
-
Singh AK, Rai A, Joshi I, Reddy DN, Guha R, Alka K, Shukla S, Rath SK, Nazir A, Clement JP, and Kundu TK
- Subjects
- Animals, Administration, Oral, Mice, p300-CBP Transcription Factors metabolism, Mice, Inbred C57BL, Long-Term Potentiation drug effects, Hippocampus drug effects, Hippocampus metabolism, Male, Spinal Cord Injuries drug therapy, Spinal Cord Injuries metabolism, Neuronal Plasticity drug effects
- Abstract
TTK21 is a small-molecule activator of p300/creb binding protein (CBP) acetyltransferase activity, which, upon conjugation with a glucose-derived carbon nanosphere (CSP), can efficiently cross the blood-brain barrier and activate histone acetylation in the brain. Its role in adult neurogenesis and retention of long-term spatial memory following intraperitoneal (IP) administration is well established. In this study, we successfully demonstrate that CSP-TTK21 can be effectively administered via oral gavage. Using a combination of molecular biology, microscopy, and electrophysiological techniques, we systematically investigate the comparative efficacy of oral administration of CSP and CSP-TTK21 in wild-type mice and evaluate their functional effects in comparison to intraperitoneal (IP) administration. Our findings indicate that CSP-TTK21, when administered orally, induces long-term potentiation in the hippocampus without significantly altering basal synaptic transmission, a response comparable to that achieved through IP injection. Remarkably, in a spinal cord injury model, oral administration of CSP-TTK21 exhibits efficacy equivalent to that of IP administration. Furthermore, our research demonstrates that oral delivery of CSP-TTK21 leads to improvements in motor function, histone acetylation dynamics, and increased expression of regeneration-associated genes (RAGs) in a spinal injury rat model, mirroring the effectiveness of IP administration. Importantly, no toxic and mutagenic effects of CSP-TTK21 are observed at a maximum tolerated dose of 1 g/kg in Sprague-Dawley (SD) rats via the oral route. Collectively, these results underscore the potential utility of CSP as an oral drug delivery system, particularly for targeting the neural system.
- Published
- 2024
- Full Text
- View/download PDF
30. Purification of ursolic acid and β-sitosterol from endophytic Alternaria alternata for their alpha-amylase inhibitory activity.
- Author
-
Dwibedi V, Mishra SS, George N, Joshi M, Kaur G, Gupta M, and Rath SK
- Subjects
- Endophytes chemistry, Hypoglycemic Agents pharmacology, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Molecular Structure, Alternaria chemistry, Triterpenes chemistry, Triterpenes pharmacology, Triterpenes isolation & purification, Ursolic Acid, Sitosterols chemistry, Sitosterols pharmacology, Sitosterols isolation & purification, Molecular Docking Simulation, alpha-Amylases antagonists & inhibitors, alpha-Amylases metabolism, alpha-Amylases chemistry, Enzyme Inhibitors pharmacology, Enzyme Inhibitors chemistry
- Abstract
Fungal endophytes are a known warehouse of bioactive compounds with multifarious applications. In the present investigation two compounds, β-Sitosterol (1) and ursolic acid (2), were isolated from Alternaria alternata , an endophytic fungus associated with Morus alba Linn for the first time. The structure of the compounds was elucidated on the basis of comprehensive spectral analysis (UV, IR, 1 H-, 13 C- and 2D-NMR, as well as HRESI-MS). In the in vitro alpha amylase inhibitory assay both compounds (1) and (2) show potent antidiabetic activity. In support, Docking studies indicate significant binding affinity of the isolated compounds. Hence from the present study, it can be concluded that endophytic fungi in Morus alba Linn can find use in antidiabetic drug development in the medicinal industry.Communicated by Ramaswamy H. Sarma.
- Published
- 2024
- Full Text
- View/download PDF
31. Deoxynivalenol Induces Drp-1-Mediated Mitochondrial Dysfunction via Elevating Oxidative Stress.
- Author
-
Mishra S, Kapoor R, Sushma, Kanchan S, Jha G, Sharma D, Tomar B, and Rath SK
- Subjects
- Humans, Dynamins metabolism, Membrane Potential, Mitochondrial drug effects, Calcium metabolism, Cell Survival drug effects, Dose-Response Relationship, Drug, Mitochondrial Dynamics drug effects, Cell Line, Tumor, Trichothecenes toxicity, Oxidative Stress drug effects, Mitochondria drug effects, Mitochondria metabolism, Reactive Oxygen Species metabolism
- Abstract
Mitochondrial dysfunction is often linked to neurotoxicity and neurological diseases and stems from oxidative stress, yet effective therapies are lacking. Deoxynivalenol (DON or vomitoxin) is one of the most common and hazardous type-B trichothecene mycotoxins, which contaminates crops used for food and animal feed. Despite the abundance of preliminary reports, comprehensive investigations are scarce to explore the relationship between these fungal metabolites and neurodegenerative disorders. The present study aimed to elucidate the precise role of DON in mitochondrial dynamics and cell death in neuronal cells. Excessive mitochondrial fission is associated with the pathology of several neurodegenerative diseases. Human SH-SY5Y cells were treated with different concentrations of DON (250-1000 ng/mL). Post 24 and 48 h DON treatment, the indexes were measured as follows: generation of reactive oxygen species (ROS), ATP levels, mitochondrial membrane potential, calcium levels, and cytotoxicity in SH-SY5Y cells. The results showed that cytotoxicity, intracellular calcium levels, and ROS in the DON-treated group increased, while the ATP levels and mitochondrial membrane potential decreased in a dose-dependent manner. With increasing DON concentrations, the expression levels of P-Drp-1, mitochondrial fission proteins Mff, and Fis-1 were elevated with reduced activities of MFN1, MFN2, and OPA1, further resulting in an increased expression of autophagic marker LC3 and beclin-1. The reciprocal relationship between mitochondrial damage and ROS generation is evident as ROS can instigate structural and functional deficiencies within the mitochondria. Consequently, the impaired mitochondria facilitate the release of ROS, thereby intensifying the cycle of damage and exacerbating the overall process. Using specific hydroxyl, superoxide inhibitors, and calcium chelators, our study confirmed that ROS and Ca2+-mediated signaling pathways played essential roles in DON-induced Drp1 phosphorylation. Therefore, ROS and mitochondrial fission inhibitors could provide critical research tools for drug development in mycotoxin-induced neurodegenerative diseases.
- Published
- 2024
- Full Text
- View/download PDF
32. Synchronized Codelivery of Combination Chemotherapies Intratumorally Using a Lipidic Lyotropic Liquid Crystal System.
- Author
-
Saklani R, Yadav PK, Tiwari AK, Gawali SL, Hassan PA, Yadav K, Mugale MN, Kalleti N, Rath SK, Mishra DP, Dierking I, and Chourasia MK
- Subjects
- Animals, Mice, Female, Paclitaxel chemistry, Paclitaxel pharmacology, Paclitaxel pharmacokinetics, Cell Line, Tumor, Humans, Glycerides chemistry, Antineoplastic Combined Chemotherapy Protocols pharmacology, Antineoplastic Combined Chemotherapy Protocols chemistry, Antineoplastic Combined Chemotherapy Protocols pharmacokinetics, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Drug Carriers chemistry, Liquid Crystals chemistry, Doxorubicin chemistry, Doxorubicin pharmacology, Mice, Inbred BALB C
- Abstract
In this work, an injectable in situ depot-forming lipidic lyotropic liquid crystal (L3C) system is developed to codeliver a precisely synchronized combination of chemotherapeutics intratumorally. The developed L3C system is composed of amphiphilic lipids and surfactants, including monoolein, phosphatidylcholine, tocopherol acetate, and d-α-tocopherol polyethylene glycol 1000 succinate. Owing to its amphiphilic nature, the developed formulation can coaccommodate both hydrophobic and hydrophilic chemotherapeutic moieties simultaneously. The study presents a proof of concept by designing a combination chemotherapy regimen in vitro and demonstrating its in vivo translation using doxorubicin and paclitaxel as model hydrophilic and hydrophobic drug moieties, respectively. The synchronized combination of the two chemotherapeutics with maximum synergistic activity was identified, coloaded in the developed L3C system at predefined stoichiometric ratios, and evaluated for antitumor efficacy in the 4T1 breast tumor model in BALB/c mice. The drug-loaded L3C formulation is a low-viscosity injectable fluid with a lamellar phase that transforms into a hexagonal mesophase depot system upon intratumoral injection. The drug-loaded depot system locally provides sustained intratumoral delivery of the chemotherapeutics combination at their precisely synchronized ratio for over a period of one month. Results demonstrate that the exposure of the tumor to the precisely synchronized intratumoral chemotherapeutics combination via the developed L3C system resulted in significantly higher antitumor activity and reduced cardiotoxicity compared to the unsynchronized combination chemotherapy or the synchronized but uncoordinated drug delivery administered by a conventional intravenous route. These findings demonstrate the potential of the developed L3C system for achieving synchronized codelivery of the chemotherapeutics combination intratumorally and improving the efficacy of combination chemotherapy.
- Published
- 2024
- Full Text
- View/download PDF
33. Isolation, chemical characterization, antimicrobial activity, and molecular docking studies of 2,6-dimethoxy benzoquinone isolated from medicinal plant Flacourtia jangomas .
- Author
-
Tomar R, Mishra SS, Sahoo J, and Rath SK
- Abstract
In the present investigation one compound, 2,6-dimethoxy benzoquinone (FJL-1), was isolated from the dichloromethane (DCM) fraction of the organic leaf extract of Flacourtia Jangomas for the first time. The compound structure was elucidated using extensive spectral analysis, including
1 H, and13 C NMR. Furthermore, the DPPH and ABTS methods were used to evaluate the antioxidant activity of the organic extract, its fractions, and the isolated compound FJL-1. Antioxidant activity of the petroleum, ether, DCM, and methanol fractions of the organic extract and the isolated compound of F. Jangomas revealed moderate to strong radical scavenging ability. Additionally, the antimicrobial activity of FJL-1 against Staphylococcus aureus (MTCC 737 and MTCC 96 strains) was observed in an inhibition zone size of 21.6 ± 0.6 to 21.7 ± 0.58 mm showing potential inhibitory activity. The isolated compound FJL-1 shows excellent binding with the 2W9S proteins in terms of docking score compared with the drug Trimethoprim, which also exhibited similar types of interaction and potency against S. aureus . The leaves of F. jangomas can be considered a great source for the identification of numerous important phytoconstituents with potential uses in nutrition, aromatherapy, and the pharmaceutical sector., Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-04002-w., Competing Interests: Conflict of interestThe authors declare no conflict of interest., (© King Abdulaziz City for Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)- Published
- 2024
- Full Text
- View/download PDF
34. Nanoparticle-mediated diagnosis, treatment, and prevention of breast cancer.
- Author
-
Leena Panigrahi L, Samal P, Ranjan Sahoo S, Sahoo B, Pradhan AK, Mahanta S, Rath SK, and Arakha M
- Abstract
By virtue of their advanced physicochemical properties, nanoparticles have attracted significant attention from researchers for application in diverse fields of medical science. Breast cancer, presenting a high risk of morbidity and mortality, frequently occurs in women and is considered a malignant tumor. Globally, breast cancer is considered the second leading cause of death. Accordingly, its poor prognosis, invasive metastasis, and relapse have motivated oncologists and nano-medical researchers to develop highly potent nanotherapies to cure this deadly disease. In this case, nanoparticles have emerged as responsive platforms for breast cancer management, providing new approaches to improve the diagnostic accuracy, deliver targeted therapies, and limit the progression of this disease. Recently, smart nano-carriers encapsulating drugs, ligands, and tracking probes have been developed for the specific therapy of breast cancers. Further, efforts have been devoted to developing various nano-systems with minimal toxicity. The aim of this review is to present a background on novel nanotheranostic methods that can be employed to diagnose and treat breast cancers and encourage readers to focus on the development of novel nanomedicine for breast cancers and other deadly diseases. In this context, we discuss different methods for the diagnosis, treatment, and prevention of breast cancers using different metal and metal oxide nanoparticles., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)
- Published
- 2024
- Full Text
- View/download PDF
35. Nanocarrier - Mediated Salinomycin Delivery Induces Apoptosis and Alters EMT Phenomenon in Prostate Adenocarcinoma.
- Author
-
Kanchan S, Marwaha D, Tomar B, Agrawal S, Mishra S, Kapoor R, Sushma, Jha G, Sharma D, Bhatta RS, Mishra PR, and Rath SK
- Subjects
- Male, Animals, Humans, Rats, Cell Line, Tumor, Cell Movement drug effects, PC-3 Cells, Drug Delivery Systems methods, Polyether Polyketides, Prostatic Neoplasms drug therapy, Prostatic Neoplasms pathology, Prostatic Neoplasms metabolism, Pyrans pharmacology, Pyrans administration & dosage, Apoptosis drug effects, Adenocarcinoma drug therapy, Adenocarcinoma pathology, Adenocarcinoma metabolism, Drug Carriers chemistry, Nanoparticles chemistry, Epithelial-Mesenchymal Transition drug effects, Rats, Wistar, Antineoplastic Agents pharmacology, Antineoplastic Agents administration & dosage
- Abstract
Salinomycin (Sal) has been recently discovered as a novel chemotherapeutic agent against various cancers including prostate cancer which is one of the most commonly diagnosed cancers affecting male populations worldwide. Herein we designed salinomycin nanocarrier (Sal-NPs) to extend its systemic circulation and to increase its anticancer potential. Prepared nanoform showed high encapsulation and sustained release profile for salinomycin. The present study elucidated the cytotoxicity and mechanism of apoptotic cell death of Sal-NPs against prostate cancer both in vitro and in vivo. At all measured concentrations, Sal-NPs showed more significant cytotoxicity to DU145 and PC3 cells than Sal alone. This effect was mediated by apoptosis, as confirmed by ROS generation, loss of MMP and cell cycle arrest at the G1 phase in both cells. Sal-NPs efficiently inhibited migration of PC3 and DU145 cells via effectively downregulating the epithelial mesenchymal transition. Also, the results confirmed that Sal-NPs can effectively inhibit the induction of Prostate adenocarcinoma in male Wistar rats. Sal-NPs treatment exhibited a decrease in tumour sizes, a reduction in prostate weight, and an increase in body weight, which suggests that Sal-NPs is more effective than salinomycin alone. Our results suggest that the molecular mechanism underlying the Sal-NPs anticancer effect may lead to the development of a potential therapeutic strategy for treating prostate adenocarcinoma., (© 2024. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists.)
- Published
- 2024
- Full Text
- View/download PDF
36. Stilbenes: a journey from folklore to pharmaceutical innovation.
- Author
-
Kaur G, Kaur R, Sodhi GK, George N, Rath SK, Walia HK, Dwibedi V, and Saxena S
- Subjects
- Humans, Fungi drug effects, Endophytes chemistry, Endophytes metabolism, Endophytes isolation & purification, Antioxidants chemistry, Antioxidants pharmacology, Medicine, Traditional, Plants chemistry, Stilbenes chemistry, Stilbenes pharmacology, Resveratrol pharmacology, Resveratrol chemistry
- Abstract
In modern times, medicine is predominantly based on evidence-based practices, whereas in ancient times, indigenous people relied on plant-based medicines with factual evidence documented in ancient books or folklore that demonstrated their effectiveness against specific infections. Plants and microbes account for 70% of drugs approved by the USFDA (U.S. Food and Drug Administration). Stilbenes, polyphenolic compounds synthesized by plants under stress conditions, have garnered significant attention for their therapeutic potential, bridging ancient wisdom with modern healthcare. Resveratrol, the most studied stilbene, initially discovered in grapes, red wine, peanuts, and blueberries, exhibits diverse pharmacological properties, including cardiovascular protection, antioxidant effects, anticancer activity, and neuroprotection. Traditional remedies, documented in ancient texts like the Ayurvedic Charak Samhita, foreshadowed the medicinal properties of stilbenes long before their modern scientific validation. Today, stilbenes are integral to the booming wellness and health supplement market, with resveratrol alone projected to reach a market value of 90 million US$ by 2025. However, challenges in stilbene production persist due to limited natural sources and costly extraction methods. Bioprospecting efforts reveal promising candidates for stilbene production, particularly endophytic fungi, which demonstrate high-yield capabilities and genetic modifiability. However, the identification of optimal strains and fermentation processes remains a critical consideration. The current review emphasizes the knowledge of the medicinal properties of Stilbenes (i.e., cardiovascular, antioxidant, anticancer, anti-inflammatory, etc.) isolated from plant and microbial sources, while also discussing strategies for their commercial production and future research directions. This also includes examples of novel stilbenes compounds reported from plant and endophytic fungi., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
- Full Text
- View/download PDF
37. Therapeutic Implications of Dietary Polyphenols-Loaded Nanoemulsions in Cancer Therapy.
- Author
-
Tomar R, Das SS, Balaga VKR, Tambe S, Sahoo J, Rath SK, Ruokolainen J, and Kesari KK
- Subjects
- Humans, Antioxidants chemistry, Signal Transduction, Polyphenols pharmacology, Polyphenols chemistry, Neoplasms metabolism
- Abstract
Cancer is one of the major causes of death worldwide, even the second foremost cause related to non-communicable diseases. Cancer cells typically possess several cellular and biological processes including, persistence, propagation, differentiation, cellular death, and expression of cellular-type specific functions. The molecular picture of carcinogenesis and progression is unwinding, and it appears to be a tangled combination of processes occurring within and between cancer cells and their surrounding tissue matrix. Polyphenols are plant secondary metabolites abundant in fruits, vegetables, cereals, and other natural plant sources. Natural polyphenols have implicated potential anticancer activity by various mechanisms involved in their antitumor action, including modulation of signaling pathways majorly related to cellular proliferation, differentiation, relocation, angiogenesis, metastatic processes, and cell death. The applications of polyphenols have been limited due to the hydrophobic nature and lower oral bioavailability that could be possibly overcome through encapsulating them into nanocarrier-mediated delivery systems, leading to improved anticancer activity. Nanoemulsions (NEs) possess diverse feasible properties, including greater surface area, modifiable surficial charge, higher half-life, site-specific targeting, and formulation imaging capability necessary to create a practical therapeutic impact, and have drawn increased attention in cancer therapy research. This review has summarized and discussed the basic concepts, classification, delivery approaches, and anticancer mechanism of various polyphenols and polyphenols-encapsulated nanoemulsions with improved cancer therapy.
- Published
- 2024
- Full Text
- View/download PDF
38. Corrigendum to "Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma" [Metabolism, Volume 152, March 2024, 155771].
- Author
-
Ishteyaque S, Singh G, Yadav KS, Verma S, Sharma RK, Sen S, Srivastav AK, Mitr K, Lahiri A, Bawankule DU, Rath SK, Kumar D, and Mugale MN
- Published
- 2024
- Full Text
- View/download PDF
39. Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma.
- Author
-
Ishteyaque S, Singh G, Yadav KS, Verma S, Sharma RK, Sen S, Srivastava AK, Mitra K, Lahiri A, Bawankule DU, Rath SK, Kumar D, and Mugale MN
- Subjects
- Animals, alpha-Fetoproteins metabolism, Cell Line, Tumor, Interleukin-6 metabolism, NF-kappa B metabolism, STAT3 Transcription Factor metabolism, Carcinoma, Hepatocellular complications, Carcinoma, Hepatocellular metabolism, Liver Neoplasms complications, Liver Neoplasms metabolism, Mitochondrial Diseases etiology, Mitochondrial Diseases metabolism, Signal Transduction
- Abstract
Background: Hepatocellular carcinoma (HCC) continues to pose a significant health challenge and is often diagnosed at advanced stages. Metabolic reprogramming is a hallmark of many cancer types, including HCC and it involves alterations in various metabolic or nutrient-sensing pathways within liver cells to facilitate the rapid growth and progression of tumours. However, the role of STAT3-NFκB in metabolic reprogramming is still not clear., Approach and Results: Diethylnitrosamine (DEN) administered animals showed decreased body weight and elevated level of serum enzymes. Also, Transmission electron microscopy (TEM) analysis revealed ultrastructural alterations. Increased phosphorylated signal transducer and activator of transcription-3 (p-STAT3), phosphorylated nuclear factor kappa B (p-NFκβ), dynamin related protein 1 (Drp-1) and alpha-fetoprotein (AFP) expression enhance the carcinogenicity as revealed in immunohistochemistry (IHC). The enzyme-linked immunosorbent assay (ELISA) concentration of IL-6 was found to be elevated in time dependent manner both in blood serum and liver tissue. Moreover, immunoblot analysis showed increased level of p-STAT3, p-NFκβ and IL-6 stimulated the upregulation of mitophagy proteins such as Drp-1, Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK-1). Meanwhile, downregulation of Poly [ADP-ribose] polymerase 1 (PARP-1) and cleaved caspase 3 suppresses apoptosis and enhanced expression of AFP supports tumorigenesis. The mRNA level of STAT3 and Drp-1 was also found to be significantly increased. Furthermore, we performed high-field 800 MHz Nuclear Magnetic Resonance (NMR) based tissue and serum metabolomics analysis to identify metabolic signatures associated with the progression of liver cancer. The metabolomics findings revealed aberrant metabolic alterations in liver tissue and serum of 75th and 105th days of intervention groups in comparison to control, 15th and 45th days of intervention groups. Tissue metabolomics analysis revealed the accumulation of succinate in the liver tissue samples, whereas, serum metabolomics analysis revealed significantly decreased circulatory levels of ketone bodies (such as 3-hydroxybutyrate, acetate, acetone, etc.) and membrane metabolites suggesting activated ketolysis in advanced stages of liver cancer., Conclusion: STAT3-NFκβ signaling axis has a significant role in mitochondrial dysfunction and metabolic alterations in the development of HCC., Competing Interests: Declaration of competing interest The authors declare that they have no competing of interest., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2024
- Full Text
- View/download PDF
40. C-30 analogues of betulinic acid as potent cytotoxic agents: design, synthesis, biological evaluation and in-silico studies.
- Author
-
Rath SK, Nagar RK, Das S, Yadav G, Mukherjee D, Singh B, Singh S, and Sangwan PL
- Abstract
In an endeavour to improve the anti-cancer activity of betulinic acid (BA), a series of C-30 derivatives were envisaged and synthesized with a novel synthetic approach. All the derivatives were evaluated for cytotoxic activity by MTT assay against six different human cancer cell lines: prostate (PC3), lung (A549), human hepatocellular carcinoma (HepG2), human leukemia (Molt-4), pancreatic (Panc-1) and breast (MCF-7). The data revealed that compound 16 was observed most promising cytotoxic agent with IC
50 values of 7.43 μM, 9.1 μM, and 9.64 μM against A549, MCF-7, and PC3 cancer cell lines respectively. A further mechanistic study confirmed compound 16 showed significant cell death by arresting the cell cycle in the G1 phase and inducing apoptosis in A549 cells.Communicated by Ramaswamy H. Sarma.- Published
- 2024
- Full Text
- View/download PDF
41. SAMHD1 expression contributes to doxorubicin resistance and predicts survival outcomes in diffuse large B-cell lymphoma patients.
- Author
-
Daddacha W, Monroe D, Schlafstein AJ, Withers AE, Thompson EB, Danelia D, Luong NC, Sesay F, Rath SK, Usoro ER, Essien ME, Jung AT, Jiang JG, Hu J, Mahboubi B, Williams A, Steinbeck JE, Yang X, Buchwald ZS, Dynan WS, Switchenko JM, Kim B, Khan MK, Jaye DL, and Yu DS
- Abstract
Diffuse large B-cell lymphoma (DLBCL) is a commonly diagnosed, aggressive non-Hodgkin's lymphoma. While R-CHOP chemoimmunotherapy is potentially curative, about 40% of DLBCL patients will fail, highlighting the need to identify biomarkers to optimize management. SAMHD1 has a dNTPase-independent role in promoting resection to facilitate DNA double-strand break (DSB) repair by homologous recombination. We evaluated the relationship of SAMHD1 levels with sensitivity to DSB-sensitizing agents in DLBCL cells and the association of SAMHD1 expression with clinical outcomes in 79 DLBCL patients treated with definitive therapy and an independent cohort dataset of 234 DLBCL patients. Low SAMHD1 expression, Vpx-mediated, or siRNA-mediated degradation/depletion in DLBCL cells was associated with greater sensitivity to doxorubicin and PARP inhibitors. On Kaplan-Meier log-rank survival analysis, low SAMHD1 expression was associated with improved overall survival (OS), which on subset analysis remained significant only in patients with advanced stage (III-IV) and moderate to high risk (2-5 International Prognostic Index (IPI)). The association of low SAMHD1 expression with improved OS remained significant on multivariate analysis independent of other adverse factors, including IPI, and was validated in an independent cohort. Our findings suggest that SAMHD1 expression mediates doxorubicin resistance and may be an important prognostic biomarker in advanced, higher-risk DLBCL patients., (© The Author(s) 2024. Published by Oxford University Press on behalf of NAR Cancer.)
- Published
- 2024
- Full Text
- View/download PDF
42. Thermal evolution of a polymer-nanoparticle binary mixture.
- Author
-
Kumar S, Rath SK, Kushwaha A, Deshpande SK, Patro TU, and Harikrishnan G
- Abstract
We experimentally probe the microscopic variations in a model polymer-nanoparticle (NP) binary mixture (mixture of polybutadiene and clay nanoplatelets) across a thermal evolution path for which T
evolution > Tg(polymer) . The evolution of the NP dispersion, NP crystallinity, polymer chain-NP interface, and nature of polymer chain-NP interaction are mapped for a spectrum of temperatures and NP concentrations constrained by experiments. Multiple pieces of evidence indicate that thermal evolution does not influence the nature of interparticle dispersion and is also independent of NP concentration in the binary mixture. However, the NP crystalline order significantly reduces across the thermal evolution path. Thermal evolution induces a transition of a sharp polymer chain-NP interface to a diffuse interfacial layer. In contrast, an already diffuse polymer-NP interface existing in the binary mixture due to particle crowding at high NP concentrations undergoes no significant change in its nature across the evolution path. At all particle concentrations, thermal evolution changes the dominant interaction from polymer chain-polymer chain to polymer chain-NP. These insights aid in explaining the molecular origins of unique and anomalous behaviors shown by polymer-nanoparticle binary mixtures while undergoing thermal evolution.- Published
- 2024
- Full Text
- View/download PDF
43. HELZ promotes R loop resolution to facilitate DNA double-strand break repair by homologous recombination.
- Author
-
Haji-Seyed-Javadi R, Koyen AE, Rath SK, Madden MZ, Hou Y, Song BS, Kenney AM, Lan L, Yao B, and Yu DS
- Abstract
R loops are RNA-DNA hybrid containing structures involved in diverse cellular processes, including DNA double-strand break (DSB) repair. R loop homeostasis involving the formation and resolution of R loops is critical for DSB repair, and its dysregulation leads to genome instability. Here we show that the HELZ helicase promotes R loop resolution to facilitate DSB repair by homologous recombination (HR). HELZ depletion causes hypersensitivity to DSB-inducing agents, and HELZ localizes and binds to DSBs. HELZ depletion further leads to genomic instability in a R loop dependent manner and the accumulation of R loops globally and at DSBs. HELZ binds to R loops in response to DSBs and promotes their resolution, thereby facilitating HR to promote genome integrity. Our findings thus define a role for HELZ in promoting the resolution of R loops critical for DSB repair by HR.
- Published
- 2023
- Full Text
- View/download PDF
44. Functional implications and therapeutic targeting of androgen response elements in prostate cancer.
- Author
-
Senapati D, Sharma V, Rath SK, Rai U, and Panigrahi N
- Subjects
- Male, Humans, Androgens genetics, Androgens metabolism, Androgens therapeutic use, Ligands, DNA, Response Elements, Cell Line, Tumor, Receptors, Androgen genetics, Receptors, Androgen metabolism, Prostatic Neoplasms drug therapy, Prostatic Neoplasms genetics, Prostatic Neoplasms metabolism
- Abstract
The androgen receptor (AR) plays an essential role in the growth and progression of prostate cancer (CaP). Ligand-activated AR inside the nucleus binds to the androgen response element (ARE) of the target genes in dimeric form and recruits transcriptional machinery to facilitate gene transcription. Pharmacological compounds that inhibit the AR action either bind to the ligand binding domain (LBD) or interfere with the interactions of AR with other co-regulatory proteins, slowing the progression of the disease. However, the emergence of resistance to conventional treatment makes clinical management of CaP difficult. Resistance has been associated with activation of androgen/AR axis that restores AR transcriptional activity. Activated AR signaling in resistance cases can be mediated by several mechanisms including AR amplification, gain-of-function AR mutations, androgen receptor variant (ARVs), intracrine androgen production, and overexpression of AR coactivators. Importantly, in castration resistant prostate cancer, ARVs lacking the LBD become constitutively active and promote hormone-independent development, underlining the need to concentrate on the other domain or the AR-DNA interface for the identification of novel actionable targets. In this review, we highlight the plasticity of AR-DNA binding and explain how fine-tuning AR's cooperative interactions with DNA translate into developing an alternative strategy to antagonize AR activity., Competing Interests: Declaration of competing interest The authors have nothing to disclose., (Copyright © 2023 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
45. Success rate of infrazygomatic crest mini-implants used for en-masse retraction of maxillary anterior teeth in first premolar extraction cases: A three-dimensional comparative prospective clinical trial between adolescents and young adults.
- Author
-
Gopal H, Das SK, Barik AK, Mishra M, Rath SK, Samal R, and Sharma G
- Subjects
- Humans, Adolescent, Young Adult, Child, Adult, Bicuspid surgery, Prospective Studies, Palate, Cone-Beam Computed Tomography, Dental Implants
- Abstract
Background: The purpose of this study was to compare the success rate of infrazygomatic mini-implants between adolescents and young adults., Methods: A total of 60 subjects of different age groups ie, (group I [adolescents]: 12-18 years, mean age: 14.9 ± 2.9 years; group II [young adults]: 19-25 years, mean age = 21.9 ± 3.1 years) were assessed in the study. En-masse retraction of maxillary anterior teeth was carried out with extraction of upper first premolars with infrazygomatic crest (IZC) mini-implants as anchorage units. Clinical parameters such as success rate, soft tissue thickness, maximum insertion torque, maximum removal torque, pain response, soft tissue response, and cone-beam computed tomography parameters such as embedded angulation, penetration depth, thickness of bone on buccal and palatal aspect of mini-implant, and peri-implant bone density were evaluated., Results: The success rate of IZC mini-implants in adolescents was found to be 96.6% and 98.3% in young adults respectively. There was no significant difference in success rate between the two groups. Intergroup comparison showed a significant difference (P < 0.05) in terms of maximum insertion torque, maximum removal torque, soft tissue thickness, cortical bone thickness, and peri-implant bone density values. Comparison between right and left side revealed a significant difference (P < 0.05) with regards to soft tissue response, soft tissue thickness, total bone thickness, cortical bone thickness, and peri-implant bone density., Conclusions: There was no significant difference in the success rate of IZC mini-implants between adolescents and young adults. Thus, the use of IZC mini-implants can be recommended in adolescents for successful orthodontic treatment., (Copyright © 2023 World Federation of Orthodontists. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
46. DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joining.
- Author
-
Head PE, Kapoor-Vazirani P, Nagaraju GP, Zhang H, Rath SK, Luong NC, Haji-Seyed-Javadi R, Sesay F, Wang SY, Duong DM, Daddacha W, Minten EV, Song B, Danelia D, Liu X, Li S, Ortlund EA, Seyfried NT, Smalley DM, Wang Y, Deng X, Dynan WS, El-Rayes B, Davis AJ, and Yu DS
- Subjects
- DNA genetics, DNA metabolism, DNA End-Joining Repair, DNA Repair, DNA-Activated Protein Kinase genetics, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Ku Autoantigen metabolism, Nuclear Proteins metabolism, Sirtuin 2 genetics, Sirtuin 2 metabolism, Humans, DNA Breaks, Double-Stranded, Protein Kinases genetics
- Abstract
DNA-dependent protein kinase (DNA-PK) plays a critical role in non-homologous end joining (NHEJ), the predominant pathway that repairs DNA double-strand breaks (DSB) in response to ionizing radiation (IR) to govern genome integrity. The interaction of the catalytic subunit of DNA-PK (DNA-PKcs) with the Ku70/Ku80 heterodimer on DSBs leads to DNA-PK activation; however, it is not known if upstream signaling events govern this activation. Here, we reveal a regulatory step governing DNA-PK activation by SIRT2 deacetylation, which facilitates DNA-PKcs localization to DSBs and interaction with Ku, thereby promoting DSB repair by NHEJ. SIRT2 deacetylase activity governs cellular resistance to DSB-inducing agents and promotes NHEJ. SIRT2 furthermore interacts with and deacetylates DNA-PKcs in response to IR. SIRT2 deacetylase activity facilitates DNA-PKcs interaction with Ku and localization to DSBs and promotes DNA-PK activation and phosphorylation of downstream NHEJ substrates. Moreover, targeting SIRT2 with AGK2, a SIRT2-specific inhibitor, augments the efficacy of IR in cancer cells and tumors. Our findings define a regulatory step for DNA-PK activation by SIRT2-mediated deacetylation, elucidating a critical upstream signaling event initiating the repair of DSBs by NHEJ. Furthermore, our data suggest that SIRT2 inhibition may be a promising rationale-driven therapeutic strategy for increasing the effectiveness of radiation therapy., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2023
- Full Text
- View/download PDF
47. Ratiometric codelivery of Paclitaxel and Baicalein loaded nanoemulsion for enhancement of breast cancer treatment.
- Author
-
Yadav PK, Saklani R, Tiwari AK, Verma S, Chauhan D, Yadav P, Rana R, Kalleti N, Gayen JR, Wahajuddin, Rath SK, Mugale MN, Mitra K, and Chourasia MK
- Subjects
- Humans, Animals, Mice, Female, Paclitaxel, Tissue Distribution, Cell Line, Tumor, Mice, Inbred BALB C, Breast Neoplasms drug therapy, Breast Neoplasms pathology, Nanoparticles
- Abstract
The most prevalent clinical option for treating cancer is combination chemotherapy. In combination therapy, assessment and optimization for obtaining a synergistic ratio could be obtained by various preclinical setups. Currently, in vitro optimization is used to get synergistic cytotoxicity while constructing combinations. Herein, we co-encapsulated Paclitaxel (PTX) and Baicalein (BCLN) with TPP-TPGS
1000 containing nanoemulsion (TPP-TPGS1000 -PTX-BCLN-NE) for breast cancer treatment. The assessment of cytotoxicity of PTX and BCLN at different molar weight ratios provided an optimized synergistic ratio (1:5). Quality by Design (QbD) approach was later applied for the optimization as well as characterization of nanoformulation for its droplet size, zeta potential and drug content. TPP-TPGS1000 -PTX-BCLN-NE significantly enhanced cellular ROS, cell cycle arrest, and depolarization of mitochondrial membrane potential in the 4T1 breast cancer cell line compared to other treatments. In the syngeneic 4T1 BALB/c tumor model, TPP-TPGS1000 -PTX-BCLN-NE outperformed other nanoformulation treatments. The pharmacokinetic, biodistribution and live imaging studies pivoted TPP-TPGS1000 -PTX-BCLN-NE enhanced bioavailability and PTX accumulation at tumor site. Later, histology studies confirmed nanoemulsion non-toxicity, expressing new opportunities and potential to treat breast cancer. These results suggested that current nanoformulation can be a potential therapeutic approach to effectively address breast cancer therapy., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
48. Harnessing the action mechanisms of microbial endophytes for enhancing plant performance and stress tolerance: current understanding and future perspectives.
- Author
-
Kaur G, Patel A, Dwibedi V, and Rath SK
- Subjects
- Agriculture, Plant Development, Endophytes physiology, Plants metabolism, Plants microbiology
- Abstract
Microbial endophytes are microorganisms that reside within plant tissues without causing any harm to their hosts. These microorganisms have been found to confer a range of benefits to plants, including increased growth and stress tolerance. In this review, we summarize the recent advances in our understanding of the mechanisms by which microbial endophytes confer abiotic and biotic stress tolerance to their host plants. Specifically, we focus on the roles of endophytes in enhancing nutrient uptake, modulating plant hormones, producing secondary metabolites, and activating plant defence responses. We also discuss the challenges associated with developing microbial endophyte-based products for commercial use, including product refinement, toxicology analysis, and prototype formulation. Despite these challenges, there is growing interest in the potential applications of microbial endophytes in agriculture and environmental remediation. With further research and development, microbial endophyte-based products have the potential to play a significant role in sustainable agriculture and environmental management., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
- Full Text
- View/download PDF
49. Simultaneous estimation of five biomarkers of neuroprotective herb Ashwagandha NMITLI-118R AF1 in rat plasma and brain using LC-ESI-MS/MS: Application to its pharmacokinetic and stability studies.
- Author
-
Dadge SD, Tiwari N, Husain A, Verma S, Agarwal A, Garg R, Rath SK, Shanker K, and Gayen JR
- Subjects
- Rats, Animals, Rats, Sprague-Dawley, Rats, Inbred WF, Plant Extracts, Brain, Reproducibility of Results, Chromatography, High Pressure Liquid methods, Tandem Mass Spectrometry methods, Withania chemistry
- Abstract
Withania Somnifera (WS) is a popular nutritional supplement in the USA, Europe, and Asia, known for its pharmacological effects on neurological disorders. However, the bioanalytical method development, validation, and pharmacokinetics of WS NMITLI-118R AF1 biomarkers Withanolide A (WLD A), Withanone (WNONE), Withanolide B (WLD B), Withaferin A (WF A), and 12 Deoxywithastramonolide (12 DEOXY) in rats have not been comprehensively explored. This study aimed to develop and validate a sensitive and selective LC-ESI-MS/MS method for these biomarkers in male Sprague Dawley rats plasma and brain matrix. Rats were divided into eight groups, each containing five rats. A plant extract of NMITLI-118R AF1 at 50 mg/kg was orally administered to the rats for in-vivo pharmacokinetic investigation. All the analytes had a linear calibration curve (r
2 > 0.999), and intra-day and inter-day precision (%) were found in the range of 2.46 - 13.71% and accuracy were within the acceptable range (±15%). The biomarkers of NMITLI-118R AF1 were found stable in in-vitro plasma and simulated gastro-intestinal fluids. The observed (Cmax ) and (Tmax ) values for the biomarkers in the systemic circulation were WLD A (5.59 ± 0.34 ng/mL, Tmax 1.00 ± 0.00 h), WNONE (6.28 ± 0.41 ng/mL, Tmax 0.95 ± 0.11 h), WLD B (6.45 ± 2.87 ng/mL, Tmax 0.95 ± 0.11 h), WF A (6.50 ± 0.27 ng/mL, Tmax 1.00 ± 0.00 h), and 12 DEOXY (5.68 ± 0.39 ng/mL, Tmax 1.00 ± 0.00 h). In contrast to the old method, our approach exhibits a lower limit of quantification (LLOQ), shorter run time (less than10 min), and enables the detection of WF A and WNONE in fresh rat plasma by other quantitative analysis of mass spectrometry (m/z) [M]+ . Shows high sample volumes for both, larger plasma volumes, costlier sample collection techniques dried blood spot (DBS), more expensive solid phase extraction techniques (SPE) and longer analysis time 14 min. Moreover, our method requires a smaller sample volume 10 µL, offers faster analysis time 4 min, and achieves a higher sensitivity 1 ng/mL. This is the first report of a comprehensive study on in-vitro and in-vivo pharmacokinetics of NMITLI-118R AF1 biomarkers, which may aid in further pre-clinical and clinical trial investigations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
50. Photocatalytic activity of biosynthesized silver nanoparticle fosters oxidative stress at nanoparticle interface resulting in antimicrobial and cytotoxic activities.
- Author
-
Sahoo B, Rath SK, Champati BB, Panigrahi LL, Pradhan AK, Nayak S, Kar BR, Jha S, and Arakha M
- Subjects
- Reactive Oxygen Species metabolism, Silver toxicity, Silver chemistry, Oxidative Stress, Anti-Bacterial Agents toxicity, Metal Nanoparticles toxicity, Metal Nanoparticles chemistry, Anti-Infective Agents toxicity, Antineoplastic Agents pharmacology
- Abstract
Inside the biological milieu, nanoparticles with photocatalytic activity have potential to trigger cell death non-specifically due to production of reactive oxygen species (ROS) upon reacting with biological entities. Silver nanoparticle (AgNP) possessing narrow band gap energy can exhibit high light absorption property and significant photocatalytic activity. This study intends to explore the effects of ROS generated due to photocatalytic activity of AgNP on antimicrobial and cytotoxic propensities. To this end, AgNP was synthesized using the principle of green chemistry from the peel extract of Punica granatum L., and was characterized using UV-Vis spectroscope, transmission electron microscope and x-ray diffraction, and so forth. The antimicrobial activity of AgNP against studied bacteria indicated that, ROS generated at AgNP interface develop stress on bacterial membrane leading to bacterial cell death, whereas Alamar Blue dye reduction assay indicated that increased cytotoxic activity with increasing concentrations of AgNP. The γH2AX activity assay revealed that increasing the concentrations of AgNP increased DNA damaging activity. The results altogether demonstrated that both antimicrobial and cytotoxic propensities are triggered primarily due interfacial ROS generation by photocatalytic AgNP, which caused membrane deformation in bacteria and DNA damage in HT1080 cells resulting in cell death., (© 2023 Wiley Periodicals LLC.)
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.