7 results on '"Ratlamwala, Huzefa"'
Search Results
2. Gene expression profiling of [CD8.sup.+] T cells predicts prognosis in patients with Crohn disease and ulcerative colitis
- Author
-
Lee, James C., Lyons, Paul A., McKinney, Eoin F., Sowerby, John M., Carr, Edward J., Bredin, Francesca, Rickman, Hannah M., Ratlamwala, Huzefa, Hatton, Alexander, Rayner, Tim F., Parkes, Miles, and Smith, Kenneth G.C.
- Subjects
T cells -- Physiological aspects -- Research ,Gene expression -- Research ,Ulcerative colitis -- Prognosis -- Genetic aspects -- Research ,Crohn's disease -- Prognosis -- Research -- Genetic aspects ,Health care industry - Abstract
Crohn disease (CD) and ulcerative colitis (UC) are increasingly common, chronic forms of inflammatory bowel disease. The behavior of these diseases varies unpredictably among patients. Identification of reliable prognostic biomarkers would enable treatment to be personalized so that patients destined to experience aggressive disease could receive appropriately potent therapies from diagnosis, while those who will experience more indolent disease are not exposed to the risks and side effects of unnecessary immunosuppression. Using transcriptional profiling of circulating T cells isolated from patients with CD and UC, we identified analogous [CD8.sup.+] T cell transcriptional signatures that divided patients into 2 otherwise indistinguishable subgroups. In both UC and CD, patients in these subgroups subsequently experienced very different disease courses. A substantially higher incidence of frequently relapsing disease was experienced by those patients in the subgroup defined by elevated expression of genes involved in antigen-dependent T cell responses, including signaling initiated by both IL-7 and TCR ligation--pathways previously associated with prognosis in unrelated autoimmune diseases. No equivalent correlation was observed with [CD4.sup.+] T cell gene expression. This suggests that the course of otherwise distinct autoimmune and inflammatory conditions may be influenced by common pathways and identifies what we believe to be the first biomarker that can predict prognosis in both UC and CD from diagnosis, a major step toward personalized therapy., Instruction Crohn disease (CD) and ulcerative colitis (UC) are chronic forms of inflammatory bowel disease (IBD) that predominantly affect young adults and cause considerable morbidity. UC typically presents with bloody [...]
- Published
- 2011
- Full Text
- View/download PDF
3. The generalist herbivore Tetranychus urticae (Koch) adapts to novel plant hosts through rapid evolution of metabolic resistance
- Author
-
Salehipourshirazi, Golnaz, Bruinsma, Kristie, Ratlamwala, Huzefa, Dixit, Sameer, Arbona, Vicent, Widemann, Emilie, Milojevic, Maja, Jin, Pengyu, Bensoussan, Nicolas, Gómez-Cadenas, Aurelio, Zhurov, Vladimir, Grbic, Miodrag, and Grbic, Vojislava
- Subjects
fungi ,food and beverages - Abstract
Genetic adaptation, occurring over long evolutionary time, enables host-specialized herbivores to develop novel resistance traits and to counteract the defenses of a narrow range of host plants. In contrast, physiological acclimation, leading to the suppression and/or detoxification of host defenses is hypothesized to enable generalists to shift between plant hosts. Here, we examined the long-term response of an extreme generalist, the two-spotted spider mite, Tetranychus urticae Koch (TSSM), to the shift to the non-preferred and novel host plant Arabidopsis thaliana . We identified the key requirement of two tiers of cytochrome P450 monooxygenases for TSSM adaptation to Arabidopsis : general xenobiotic-responsive P450s that have a limited contribution to mite adaptation to Arabidopsis and adaptation-associated P450s that efficiently counteract Arabidopsis defenses, illustrating that in about 25 generations of TSSM selection on Arabidopsis plants mites evolved metabolic resistances characteristic of both generalist and specialist herbivores.
- Published
- 2020
- Full Text
- View/download PDF
4. Rapid specialization of counter defenses enables two-spotted spider mite to adapt to novel plant hosts
- Author
-
Salehipourshirazi, Golnaz, primary, Bruinsma, Kristie, additional, Ratlamwala, Huzefa, additional, Dixit, Sameer, additional, Arbona, Vicent, additional, Widemann, Emilie, additional, Milojevic, Maja, additional, Jin, Pengyu, additional, Bensoussan, Nicolas, additional, Gómez-Cadenas, Aurelio, additional, Zhurov, Vladimir, additional, Grbic, Miodrag, additional, and Grbic, Vojislava, additional
- Published
- 2020
- Full Text
- View/download PDF
5. Gene expression profiling of CD8+ T cells predicts prognosis in patients with Crohn disease and ulcerative colitis
- Author
-
Lee, James C, Lyons, Paul A, McKinney, Eoin F, Sowerby, John M, Carr, Edward J, Bredin, Francesca, Rickman, Hannah M, Ratlamwala, Huzefa, Hatton, Alexander, Rayner, Tim F, Parkes, Miles, and Smith, Kenneth GC
- Subjects
Adult ,Male ,Interleukin-7 ,Receptors, Antigen, T-Cell ,CD8-Positive T-Lymphocytes ,Middle Aged ,Prognosis ,Crohn Disease ,Humans ,Colitis, Ulcerative ,Female ,Prospective Studies ,Transcriptome ,Signal Transduction - Abstract
Crohn disease (CD) and ulcerative colitis (UC) are increasingly common, chronic forms of inflammatory bowel disease. The behavior of these diseases varies unpredictably among patients. Identification of reliable prognostic biomarkers would enable treatment to be personalized so that patients destined to experience aggressive disease could receive appropriately potent therapies from diagnosis, while those who will experience more indolent disease are not exposed to the risks and side effects of unnecessary immunosuppression. Using transcriptional profiling of circulating T cells isolated from patients with CD and UC, we identified analogous CD8+ T cell transcriptional signatures that divided patients into 2 otherwise indistinguishable subgroups. In both UC and CD, patients in these subgroups subsequently experienced very different disease courses. A substantially higher incidence of frequently relapsing disease was experienced by those patients in the subgroup defined by elevated expression of genes involved in antigen-dependent T cell responses, including signaling initiated by both IL-7 and TCR ligation - pathways previously associated with prognosis in unrelated autoimmune diseases. No equivalent correlation was observed with CD4+ T cell gene expression. This suggests that the course of otherwise distinct autoimmune and inflammatory conditions may be influenced by common pathways and identifies what we believe to be the first biomarker that can predict prognosis in both UC and CD from diagnosis, a major step toward personalized therapy.
- Published
- 2018
- Full Text
- View/download PDF
6. Two spotted spider mite (Tetranychus urticae) selection to Arabidopsis thaliana
- Author
-
Ratlamwala, Huzefa
- Subjects
herbivore-plant interaction ,integumentary system ,Arabidopsis thaliana ,indole glucosinolates ,Ecology and Evolutionary Biology ,Plant Sciences ,host plant resistance ,food and beverages ,Agriculture ,Genetics and Genomics ,Tetranychus urticae ,respiratory tract diseases ,immune system diseases ,parasitic diseases ,qRT-PCR ,Biology - Abstract
Spider mite feeding on A. thaliana induces the production of indole glucosinolates (IGs), plant secondary metabolites that negatively affect mite performance. In this study I conducted selection experiments on A. thaliana with varying levels of IGs, to determine if mites could adapt to IGs and other defense compounds. After 12 months, mites reared on host with IGs performed significantly better on A. thaliana than mites maintained on beans. However, an adaptation cost was detected between selected mite lines and their ancestral host. The qRT-PCR data on different mite lines revealed that the detoxification genes previously identified may only be involved in general stress response to IGs and mites do not interfere with A. thaliana defense response. Thus, performing the entire transcriptome profile of selected mites can help to better understand the molecular mechanisms involved in mites’ ability to develop resistance to IGs, but also to other defense compounds in A. thaliana.
- Published
- 2014
7. Gene expression profiling of CD8+ T cells predicts prognosis in patients with Crohn disease and ulcerative colitis
- Author
-
Lee, James C, Lyons, Paul A, McKinney, Eoin F, Sowerby, John M, Carr, Edward J, Bredin, Francesca, Rickman, Hannah M, Ratlamwala, Huzefa, Hatton, Alexander, Rayner, Tim F, Parkes, Miles, Smith, Kenneth GC, Lee, James [0000-0001-5711-9385], Lyons, Paul [0000-0001-7035-8997], McKinney, Eoin [0000-0003-3516-3072], Carr, Edward [0000-0001-9343-4593], Parkes, Miles [0000-0002-6467-0631], Smith, Kenneth [0000-0003-3829-4326], and Apollo - University of Cambridge Repository
- Subjects
Adult ,Male ,Interleukin-7 ,Receptors, Antigen, T-Cell ,CD8-Positive T-Lymphocytes ,Middle Aged ,Prognosis ,Crohn Disease ,Humans ,Colitis, Ulcerative ,Female ,Prospective Studies ,Transcriptome ,Signal Transduction - Abstract
Crohn disease (CD) and ulcerative colitis (UC) are increasingly common, chronic forms of inflammatory bowel disease. The behavior of these diseases varies unpredictably among patients. Identification of reliable prognostic biomarkers would enable treatment to be personalized so that patients destined to experience aggressive disease could receive appropriately potent therapies from diagnosis, while those who will experience more indolent disease are not exposed to the risks and side effects of unnecessary immunosuppression. Using transcriptional profiling of circulating T cells isolated from patients with CD and UC, we identified analogous CD8+ T cell transcriptional signatures that divided patients into 2 otherwise indistinguishable subgroups. In both UC and CD, patients in these subgroups subsequently experienced very different disease courses. A substantially higher incidence of frequently relapsing disease was experienced by those patients in the subgroup defined by elevated expression of genes involved in antigen-dependent T cell responses, including signaling initiated by both IL-7 and TCR ligation - pathways previously associated with prognosis in unrelated autoimmune diseases. No equivalent correlation was observed with CD4+ T cell gene expression. This suggests that the course of otherwise distinct autoimmune and inflammatory conditions may be influenced by common pathways and identifies what we believe to be the first biomarker that can predict prognosis in both UC and CD from diagnosis, a major step toward personalized therapy.
- Published
- 2011
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.