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Abstract The family Sarcophagidae is very diverse in Brazil. Due to their living habits, they are the subject of many medical, veterinary, sanitary, and entomological studies. However, Sarcophagidae species are still poorly studied in forensic entomology, although they are frequently reported in carcasses and even human corpses. Thus, this study aims to identify and compare the developmental stages and intrapuparial morphological characteristics of Peckia (Euboettcheria) collusor to serve as an auxiliary tool in forensic entomology. The pupae collected after zero hour at 27 °C and 32 °C were sacrificed every three hours until the first 24 h and then every six hours until the emergence of the first adults, using 30 pupae each time, totaling 1560 for 27 °C and 1290 for 32 °C. The intrapuparial development time of this fly species under laboratory-controlled conditions was 288 h at 27 °C and 228 h at 32 °C. The 2820 pupae were analyzed according to temperature and classified into eight possible stages. This contributed to the selection of 16 key morphological characteristics to identify the age of the pupae. The identified intrapupal morphological characteristics have great potential to help researchers, experts, technical assistants, and forensic entomologists estimate the minimum post-mortem interval (minPMI) of cadavers.

Diverse larval habitats significantly influence female mosquito oviposition. Utilizing traps that simulate these habitats is helpful in the study of the bioecology and characteristics of pathogen-transmitting species during oviposition. This study evaluated the feasibility of different traps in natural environments by comparing sampling methods and detecting the oviposition of epidemiologically important mosquitoes, with emphasis on Haemagogus species, in a fragment of the Atlantic Forest in Silva Jardim, Rio de Janeiro State, Brazil. Monthly collections were conducted from March 2021 to October 2023 using four types of traps: plastic containers, tires, bamboo, and sapucaia. Immatures were collected from these traps using a pipette, placed in plastic bags, and transported to the laboratory. Tire was the most efficient trap, showing the highest mosquito abundance (n = 1239) and number of species (S = 11). Conversely, the plastic container trap exhibited the lowest diversity (H = 0.43), with only two species and a low mosquito abundance (n = 26). The bamboo trap captured six species and recorded the second-highest diversity index (H = 1.04), while the sapucaia trap captured five species and had the third-highest diversity index (H = 0.91). Of the total immatures collected, 1817 reached adulthood, comprising 13 species, two of which are vectors of the sylvatic yellow fever virus: Haemagogus leucocelaenus and Haemagogus janthinomys. In conclusion, detecting key vectors of the sylvatic yellow fever virus in Brazil highlights the need for ongoing entomological and epidemiological surveillance in the study area and its vicinity. These efforts are crucial for monitoring vector presence and activity, identifying potential transmission hotspots, and devising effective control and prevention strategies.

Although tropical forests are home to most of the global diversity, they suffer from the most significant knowledge gaps concerning their fauna. Despite its high biodiversity, Brazil is facing an alarming destruction of habitats, with species becoming extinct before they can be discovered or described via science. Therefore, there is an urgent need to expand wildlife inventories, including entomofauna surveys. The present study aimed to analyze the bionomic aspects and the influence of abiotic factors on mosquito fauna whose immature phases develop in two bamboo species, Guadua tagoara and Bambusa vulgaris, in Tijuca National Park, Rio de Janeiro, Brazil. Immatures were collected in 10 artificially drilled bamboo plants, in five stalk internodes per plant, at two sampling points, from March 2022 to March 2023, during 23 collections. A total of 1845 immatures were obtained, 72.14% at sampling point 1 and 27.86% at sampling point 2. Of this, 1162 individuals reached adulthood, belonging to the following species: Culex iridescens, Culex neglectus, Haemagogus leucocelaenus, Orthopodomyia albicosta, Sabethes identicus, Sabethes melanonymphe, Sabethes purpureus, Toxorhynchites bambusicola, Toxorhynchites sp., Trichoprosopon compressum, Trichoprosopon pallidiventer, Wyeomyia arthrostigma, Wyeomyia codiocampa, Wyeomyia lutzi, Wyeomyia oblita, Wyeomyia personata, Wyeomyia serrata, and Wyeomyia sp. The Tijuca National Park is a tourist spot and receives a large number of visitors. Thus, humans can become an accessible food source for mosquitoes in this area, making the species survey critical since important arbovirus vectors have been recorded in Rio de Janeiro.

Culicid species, which include potential vectors of yellow fever, are diverse and abundant, with species commonly co-occurring in certain sites. Studying these species can provide important insights into their vector potential and, consequently, epizootic cycles of arboviruses carried about by these vectors. Here, we evaluated the vertical distribution and temporal segregation of mosquito oviposition with emphasis on arbovirus vectors in a fragment of the Atlantic Forest in Casimiro de Abreu, Rio de Janeiro, Brazil. Two sampling points were selected: Fazenda Três Montes and the Reserva Natural de Propriedade Privada Morro Grande. Collections were carried out at two sites using 10 ovitraps installed on the vegetation cover at different heights (0, 2, 4, 6, and 8 m above ground level) and monitored monthly from July 2018 to December 2020. The hypotheses of temporal and vertical stratification were tested through a PERMANOVA, and the relationship of each species with the vertical distribution was evaluated individually through a correlation analysis. We collected a total of 3075 eggs, including four species of medical importance: Haemagogus leucocelaenus (n = 1513), Haemagogus janthinomys (n = 16), Aedes albopictus (n = 1097), and Aedes terrens (n = 449). We found that Hg. leucocelaenus had a positive relationship with height, exhibiting behavior that appears to benefit from higher heights. The abundance of Ae. terrens seemed to follow Hg. leucocelaenus, although we did not find a relationship with height for the former species. On the other hand, Ae. albopictus exhibited a negative relationship with height, becoming absent or outnumbered at higher strata. Our study site has already presented evidence of recent transmission of the wild yellow fever virus, supporting the need to carefully monitor the emergence of febrile diseases among residents in the surrounding areas and the local population.

Some Aedinii mosquitoes are of high importance in the transmission of the sylvatic YFV. Usually, their eggs are very resistant and depend on the rain for their hatching. The present study evaluated the effect of multiple mosquito-egg immersions and the sex ratio of male and female specimens from Atlantic Forest remnants in the state of Rio de Janeiro, Brazil. Three sampling sites were selected in the municipality of Casimiro de Abreu, where 50 ovitraps were randomly installed to collect eggs from the ground level up to different heights, from August 2018 to December 2020. The mosquito sex ratios were compared between seasons and forest sites, using the generalized linear mixed model (GLMM), which included sampling months and trees as random effects. A total of 33,091 mosquito eggs were collected, of which 6152 eggs were already hatched (18%) and 26,939 were unhatched; of these, approximately 76% subsequently hatched. We found that 25% of the eggs corresponded to four species: Aedes albopictus (n = 1277), Ae. terrens (n = 793), Haemagogus janthinomys (n = 89), and Hg. leucocelaenus (n = 3033). The sex ratio (male:female) was variable concerning the sampling sites and the season. For most species, GLMM estimates found no difference in the variation of the average sex ratio as a function of these predictors, and there was no evidence of temporal autocorrelation in the mosquito data. The number of immersions necessary for hatching the eggs differed between mosquito species, and eggs collected in the dry season hatched both in the first immersions and the subsequent events. Co-occurrence of Aedes terrens and Hg. leucocelaenus was the most frequently observed pairwise species combination. Considering recurrent arbovirus outbreaks in Brazil and their burden on the human population, our study helps to shed light on how these vectors behave in nature; therefore, they can be used in surveillance programs.

O artigo, trata sobre “A escrevivência de experiências formativas de uma professora no Projeto Gavião, com objetivo de analisar as experiências de formação de professores(as) no âmbito do projeto, observando a importância, as dificuldades e os impactos produzidos na vida da professora formada pelo Projeto Gavião. Metodologicamente seguimos a orientação das narrativas orais, trazidas por Josso (2004) como ferramenta importante de investigação sobre a formação de professores, a partir das quais, passamos ao processo de escrevivência, enquanto escrita do cotidiano, lembranças e memórias de trajetórias, com base em Evaristo (2006). Para isso utilizamos a entrevista semiestruturada, via Googlemeet. Explorando a escrevivência das experiências formativas no Projeto Gavião, os conceitos centrais ancoram-se em Josso (2004), Freire (1987), (1996), Arroyo (2013), Nóvoa (1995), Pacheco (2009) e Silva [et al] (2017). O texto traz como uma de suas autoras a professora formada no referido projeto que viveu essa experiência formativa, e suas narrativas giram em torno desse processo, forjado entre lutas, dificuldades, necessidades e autoidentificação do Ser Professor/a em comunidades rurais. Conclui apontando sobre a importância e os principais impactos e transformações ocorridas em sua vida pessoal e profissional, após a vivência dessa experiência, talvez no mais significativo projeto de formação de professores implementado na Amazônia paraense nas últimas HE LIFE-BASED WRITING OF FORMATIVE EXPERIENCES OF A TEACHER IN THE GAVIÃO PROJECT ABSTRACT:The article produced here addresses “The life-based writing of formative experiences of a teacher in the Gavião Project”, with the purpose of analyzing the experiences of teacher training in the scope of the project, observing the importance, the difficulties, and the impacts produced in the life of the teacher trained by Gavião Project. Methodologically, we follow the orientation of the oral narratives, brought by Josso (2004) as an important tool for research on teacher training, from which, we move on to the process of life-based writing, as the writing of everyday life, memories and remembrance of trajectories, based on Evaristo (2006). Therefore, we used the semi-structured interview, via Google meet. Exploring the life-based writing of formative experiences in the Gavião Project, the central concepts are anchored in Josso (2004), Freire (1987), (1996), Arroyo (2013), Nóvoa (1995), Pacheco (2009) and Silva [et al] (2017). The text brings as one of its authors the teacher trained in the referred project, who lived this formative experience, and her narratives revolve around this process, forged among struggles, difficulties, needs and self-identification of being a teacher in rural communities. It concludes by pointing out the importance and the main impacts and transformations that occurred in her personal and professional life, after living this experience, perhaps in the most significant teacher training project implemented in the Amazon of Pará in the last decades. KEYWORDS: Life-based writing. Teacher training. Gavião Project. LA ESCRITURA DE LAS EXPERIENCIAS FORMATIVAS DE UN PROFESOR EN EL PROYECTO GAVIÃO RESUMEN: El artículo aquí elaborado trata de “La escritura de las experiencias de formación de un docente en el Proyecto Gavião, con el objetivo de analizar las experiencias de formación docente dentro del proyecto, notando la importancia, dificultades e impactos producidos en la vida del docente capacitado para el Proyecto Gavião. Metodológicamente, seguimos la orientación de las narrativas orales, traída por Josso (2004) como una importante herramienta de investigación sobre la formación del profesorado, desde la cual, pasamos al proceso de escritura, como escritura diaria, recuerdos y recuerdos de trayectoria, a partir de Evaristo (2006). Para ello utilizamos la entrevista semiestructurada, vía Google meet. Explorando el escrutinio de las experiencias formativas en el Proyecto Gavião, los conceptos centrales están anclados en Josso (2004), Freire (1987), (1996), Arroyo (2013), Nóvoa (1995), Pacheco (2009) y Silva [et al] (2017). El texto trae como uno de sus autores a la docente formada en el referido proyecto que vivióesta experiencia formativa, y sus narrativas giran en torno a este proceso, forjado entre luchas, dificultades, necesidades y autoidentificación del Ser Docente en las comunidades rurales. Concluye señalando la importancia y los principales impactos y cambios que se han producido en su vida personal y profesional, luego de vivir esta experiencia, quizás en el proyecto de formación docente más significativo implementado en la Amazonía de Pará en las últimas décadas. PALABRASCLAVE: Escrivivência. Formación de profesores. Proyecto Gavião.