31 results on '"Reczyńska, K."'
Search Results
2. C169 THE MODELING OF RENAL ARTERY PATHWAYS
- Author
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Drewniak, T.D., Rzepecki, M., Reczynska, K., Pyryt, K., Piekla, K., Jakubowska, M., and Juszczak, K.
- Published
- 2012
- Full Text
- View/download PDF
3. Directional turnover towards larger-ranged plants over time and across habitats.
- Author
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Staude, I.R., Pereira, H.M., Daskalova, G.N., Bernhardt-Römermann, M., Diekmann, M., Pauli, H., Van Calster, H., Vellend, M., Bjorkman, A.D., Brunet, J., De Frenne, P., Hédl, R., Jandt, U., Lenoir, J., Myers-Smith, I.H., Verheyen, K., Wipf, S., Wulf, M., Andrews, C., Barančok, P., Barni, E., Benito-Alonso, J-L., Bennie, J., Berki, I., Blüml, V., Chudomelová, M., Decocq, G., Dick, J.T.A., Dirnböck, T., Durak, T., Eriksson, O., Erschbamer, B., Graae, B.J., Heinken, T., Schei, F.H., Jaroszewicz, B., Kopecký, M., Kudernatsch, T., Macek, M., Malicki, M., Máliš, F., Michelsen, O., Naaf, T., Nagel, T.A., Newton, Adrian, Nicklas, L., Oddi, L., Ortmann-Ajkai, A., Palaj, A., Petraglia, A., Petřík, P., Pielech, R., Porro, F., Puşcaş, M., Reczyńska, K., Rixen, C., Schmidt, W., Standovár, T., Steinbauer, K., Świerkosz, K., Teleki, B., Theurillat, J-P., Turtureanu, P.D., Ursu, T-M., Vanneste, T., Vergeer, P., Vild, O., Villar, L., Vittoz, P., Winkler, M., Baeten, L., Staude, I.R., Pereira, H.M., Daskalova, G.N., Bernhardt-Römermann, M., Diekmann, M., Pauli, H., Van Calster, H., Vellend, M., Bjorkman, A.D., Brunet, J., De Frenne, P., Hédl, R., Jandt, U., Lenoir, J., Myers-Smith, I.H., Verheyen, K., Wipf, S., Wulf, M., Andrews, C., Barančok, P., Barni, E., Benito-Alonso, J-L., Bennie, J., Berki, I., Blüml, V., Chudomelová, M., Decocq, G., Dick, J.T.A., Dirnböck, T., Durak, T., Eriksson, O., Erschbamer, B., Graae, B.J., Heinken, T., Schei, F.H., Jaroszewicz, B., Kopecký, M., Kudernatsch, T., Macek, M., Malicki, M., Máliš, F., Michelsen, O., Naaf, T., Nagel, T.A., Newton, Adrian, Nicklas, L., Oddi, L., Ortmann-Ajkai, A., Palaj, A., Petraglia, A., Petřík, P., Pielech, R., Porro, F., Puşcaş, M., Reczyńska, K., Rixen, C., Schmidt, W., Standovár, T., Steinbauer, K., Świerkosz, K., Teleki, B., Theurillat, J-P., Turtureanu, P.D., Ursu, T-M., Vanneste, T., Vergeer, P., Vild, O., Villar, L., Vittoz, P., Winkler, M., and Baeten, L.
- Abstract
Species turnover is ubiquitous. However, it remains unknown whether certain types of species are consistently gained or lost across different habitats. Here, we analysed the trajectories of 1827 plant species over time intervals of up to 78 years at 141 sites across mountain summits, forests, and lowland grasslands in Europe. We found, albeit with relatively small effect sizes, displacements of smaller- by larger-ranged species across habitats. Communities shifted in parallel towards more nutrient-demanding species, with species from nutrient-rich habitats having larger ranges. Because these species are typically strong competitors, declines of smaller-ranged species could reflect not only abiotic drivers of global change, but also biotic pressure from increased competition. The ubiquitous component of turnover based on species range size we found here may partially reconcile findings of no net loss in local diversity with global species loss, and link community-scale turnover to macroecological processes such as biotic homogenisation.
4. Long-term nitrogen deposition reduces the diversity of nitrogen-fixing plants.
- Author
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Moreno-García P, Montaño-Centellas F, Liu Y, Reyes-Mendez EY, Jha RR, Guralnick RP, Folk R, Waller DM, Verheyen K, Baeten L, Becker-Scarpitta A, Berki I, Bernhardt-Römermann M, Brunet J, Van Calster H, Chudomelová M, Closset D, De Frenne P, Decocq G, Gilliam FS, Grytnes JA, Hédl R, Heinken T, Jaroszewicz B, Kopecký M, Lenoir J, Macek M, Máliš F, Naaf T, Orczewska A, Petřík P, Reczyńska K, Schei FH, Schmidt W, Stachurska-Swakoń A, Standovár T, Świerkosz K, Teleki B, Vild O, and Li D
- Subjects
- Forests, Climate Change, United States, Europe, Ecosystem, Nitrogen metabolism, Nitrogen Fixation, Biodiversity, Plants metabolism, Phylogeny
- Abstract
Biological nitrogen fixation is a fundamental part of ecosystem functioning. Anthropogenic nitrogen deposition and climate change may, however, limit the competitive advantage of nitrogen-fixing plants, leading to reduced relative diversity of nitrogen-fixing plants. Yet, assessments of changes of nitrogen-fixing plant long-term community diversity are rare. Here, we examine temporal trends in the diversity of nitrogen-fixing plants and their relationships with anthropogenic nitrogen deposition while accounting for changes in temperature and aridity. We used forest-floor vegetation resurveys of temperate forests in Europe and the United States spanning multiple decades. Nitrogen-fixer richness declined as nitrogen deposition increased over time but did not respond to changes in climate. Phylogenetic diversity also declined, as distinct lineages of N-fixers were lost between surveys, but the "winners" and "losers" among nitrogen-fixing lineages varied among study sites, suggesting that losses are context dependent. Anthropogenic nitrogen deposition reduces nitrogen-fixing plant diversity in ways that may strongly affect natural nitrogen fixation.
- Published
- 2024
- Full Text
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5. Unexpected westward range shifts in European forest plants link to nitrogen deposition.
- Author
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Sanczuk P, Verheyen K, Lenoir J, Zellweger F, Lembrechts JJ, Rodríguez-Sánchez F, Baeten L, Bernhardt-Römermann M, De Pauw K, Vangansbeke P, Perring MP, Berki I, Bjorkman AD, Brunet J, Chudomelová M, De Lombaerde E, Decocq G, Dirnböck T, Durak T, Greiser C, Hédl R, Heinken T, Jandt U, Jaroszewicz B, Kopecký M, Landuyt D, Macek M, Máliš F, Naaf T, Nagel TA, Petřík P, Reczyńska K, Schmidt W, Standovár T, Staude IR, Świerkosz K, Teleki B, Vanneste T, Vild O, Waller D, and De Frenne P
- Subjects
- Europe, Trees metabolism, Biodiversity, Climate Change, Forests, Nitrogen metabolism, Plant Dispersal, Air Pollution
- Abstract
Climate change is commonly assumed to induce species' range shifts toward the poles. Yet, other environmental changes may affect the geographical distribution of species in unexpected ways. Here, we quantify multidecadal shifts in the distribution of European forest plants and link these shifts to key drivers of forest biodiversity change: climate change, atmospheric deposition (nitrogen and sulfur), and forest canopy dynamics. Surprisingly, westward distribution shifts were 2.6 times more likely than northward ones. Not climate change, but nitrogen-mediated colonization events, possibly facilitated by the recovery from past acidifying deposition, best explain westward movements. Biodiversity redistribution patterns appear complex and are more likely driven by the interplay among several environmental changes than due to the exclusive effects of climate change alone.
- Published
- 2024
- Full Text
- View/download PDF
6. Evaluating plant lineage losses and gains in temperate forest understories: a phylogenetic perspective on climate change and nitrogen deposition.
- Author
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Padullés Cubino J, Lenoir J, Li D, Montaño-Centellas FA, Retana J, Baeten L, Bernhardt-Römermann M, Chudomelová M, Closset D, Decocq G, De Frenne P, Diekmann M, Dirnböck T, Durak T, Hédl R, Heinken T, Jaroszewicz B, Kopecký M, Macek M, Máliš F, Naaf T, Orczewska A, Petřík P, Pielech R, Reczyńska K, Schmidt W, Standovár T, Świerkosz K, Teleki B, Verheyen K, Vild O, Waller D, Wulf M, and Chytrý M
- Subjects
- Phylogeny, Climate Change, Forests, Plants, Biodiversity, Nitrogen
- Abstract
Global change has accelerated local species extinctions and colonizations, often resulting in losses and gains of evolutionary lineages with unique features. Do these losses and gains occur randomly across the phylogeny? We quantified: temporal changes in plant phylogenetic diversity (PD); and the phylogenetic relatedness (PR) of lost and gained species in 2672 semi-permanent vegetation plots in European temperate forest understories resurveyed over an average period of 40 yr. Controlling for differences in species richness, PD increased slightly over time and across plots. Moreover, lost species within plots exhibited a higher degree of PR than gained species. This implies that gained species originated from a more diverse set of evolutionary lineages than lost species. Certain lineages also lost and gained more species than expected by chance, with Ericaceae, Fabaceae, and Orchidaceae experiencing losses and Amaranthaceae, Cyperaceae, and Rosaceae showing gains. Species losses and gains displayed no significant phylogenetic signal in response to changes in macroclimatic conditions and nitrogen deposition. As anthropogenic global change intensifies, temperate forest understories experience losses and gains in specific phylogenetic branches and ecological strategies, while the overall mean PD remains relatively stable., (© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
- Published
- 2024
- Full Text
- View/download PDF
7. Combining multiple investigative approaches to unravel functional responses to global change in the understorey of temperate forests.
- Author
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Landuyt D, Perring MP, Blondeel H, De Lombaerde E, Depauw L, Lorer E, Maes SL, Baeten L, Bergès L, Bernhardt-Römermann M, Brūmelis G, Brunet J, Chudomelová M, Czerepko J, Decocq G, den Ouden J, De Frenne P, Dirnböck T, Durak T, Fichtner A, Gawryś R, Härdtle W, Hédl R, Heinrichs S, Heinken T, Jaroszewicz B, Kirby K, Kopecký M, Máliš F, Macek M, Mitchell FJG, Naaf T, Petřík P, Reczyńska K, Schmidt W, Standovár T, Swierkosz K, Smart SM, Van Calster H, Vild O, Waller DM, Wulf M, and Verheyen K
- Subjects
- Trees, Plants, Nitrogen, Ecosystem, Forests
- Abstract
Plant communities are being exposed to changing environmental conditions all around the globe, leading to alterations in plant diversity, community composition, and ecosystem functioning. For herbaceous understorey communities in temperate forests, responses to global change are postulated to be complex, due to the presence of a tree layer that modulates understorey responses to external pressures such as climate change and changes in atmospheric nitrogen deposition rates. Multiple investigative approaches have been put forward as tools to detect, quantify and predict understorey responses to these global-change drivers, including, among others, distributed resurvey studies and manipulative experiments. These investigative approaches are generally designed and reported upon in isolation, while integration across investigative approaches is rarely considered. In this study, we integrate three investigative approaches (two complementary resurvey approaches and one experimental approach) to investigate how climate warming and changes in nitrogen deposition affect the functional composition of the understorey and how functional responses in the understorey are modulated by canopy disturbance, that is, changes in overstorey canopy openness over time. Our resurvey data reveal that most changes in understorey functional characteristics represent responses to changes in canopy openness with shifts in macroclimate temperature and aerial nitrogen deposition playing secondary roles. Contrary to expectations, we found little evidence that these drivers interact. In addition, experimental findings deviated from the observational findings, suggesting that the forces driving understorey change at the regional scale differ from those driving change at the forest floor (i.e., the experimental treatments). Our study demonstrates that different approaches need to be integrated to acquire a full picture of how understorey communities respond to global change., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
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8. Differentiation of natural scrub communities of the Cotoneastro-Amelanchieretum group in Central Europe.
- Author
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Świerkosz K and Reczyńska K
- Subjects
- Europe, Soil, Temperature, Ecosystem, Plants
- Abstract
Most of Central European rocky scrub communities formed by Cotoneaster integerrimus, Juniperus communis and Amelanchier ovalis are included in the association Cotoneastro-Amelanchieretum (= Junipero-Cotoneasteretum). However, this leads to the creation of syntaxon whose internal diversity is so great that it seems necessary to examine validity of its existence in the current form. This diversity entails species composition, habitat requirements and geographical distribution. Therefore, we posed the following objectives: i) to investigate the variability of species composition of the rocky scrub; (ii) to determine if there are ecological differences between the communities distinguished by species variability; (iii) to determine the geographical ranges of individual syntaxa. Altogether we analyzed 387 phytosociological relevés from Central Europe. Vegetation types of rocky scrubs were identified using the unsupervised K-means algorithm and detrended correspondence analysis. Mean Ellenberg's indicator values were applied to identify the environmental gradients shaping the plant communities. Obtained results confirmed the validity of dividing this broadly defined syntaxon into six distinct vegetation units. In order to present the studied communities in a broader context, we included into our analyses other rocky scrub with the occurrence of Cotoneaster sp. and A. ovalis, which formed the remaining three clusters. The observed differences in species composition were further supported by significant differences in soil reaction, temperature and continentality between the syntaxa. Moreover, the distinguished communities differed among one another in geographical range. Our study provides a new typology of the selected type of natural rocky scrub vegetation in Central Europe which involves environmental aspects, hence better reflects community-habitat relationships. This study also indicates a need for further revision of the classification of other types of natural scrub communities. Such classification should be based on modern data analysis methods and should primarily focus on lower vegetation units at pan-European scale., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2022
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9. Directional turnover towards larger-ranged plants over time and across habitats.
- Author
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Staude IR, Pereira HM, Daskalova GN, Bernhardt-Römermann M, Diekmann M, Pauli H, Van Calster H, Vellend M, Bjorkman AD, Brunet J, De Frenne P, Hédl R, Jandt U, Lenoir J, Myers-Smith IH, Verheyen K, Wipf S, Wulf M, Andrews C, Barančok P, Barni E, Benito-Alonso JL, Bennie J, Berki I, Blüml V, Chudomelová M, Decocq G, Dick J, Dirnböck T, Durak T, Eriksson O, Erschbamer B, Graae BJ, Heinken T, Schei FH, Jaroszewicz B, Kopecký M, Kudernatsch T, Macek M, Malicki M, Máliš F, Michelsen O, Naaf T, Nagel TA, Newton AC, Nicklas L, Oddi L, Ortmann-Ajkai A, Palaj A, Petraglia A, Petřík P, Pielech R, Porro F, Puşcaş M, Reczyńska K, Rixen C, Schmidt W, Standovár T, Steinbauer K, Świerkosz K, Teleki B, Theurillat JP, Turtureanu PD, Ursu TM, Vanneste T, Vergeer P, Vild O, Villar L, Vittoz P, Winkler M, and Baeten L
- Subjects
- Ecosystem, Forests, Plants, Biodiversity, Grassland
- Abstract
Species turnover is ubiquitous. However, it remains unknown whether certain types of species are consistently gained or lost across different habitats. Here, we analysed the trajectories of 1827 plant species over time intervals of up to 78 years at 141 sites across mountain summits, forests, and lowland grasslands in Europe. We found, albeit with relatively small effect sizes, displacements of smaller- by larger-ranged species across habitats. Communities shifted in parallel towards more nutrient-demanding species, with species from nutrient-rich habitats having larger ranges. Because these species are typically strong competitors, declines of smaller-ranged species could reflect not only abiotic drivers of global change, but also biotic pressure from increased competition. The ubiquitous component of turnover based on species range size we found here may partially reconcile findings of no net loss in local diversity with global species loss, and link community-scale turnover to macroecological processes such as biotic homogenisation., (© 2021 The Authors. Ecology Letters published by John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
10. Surface modification of polyurethane with eptifibatide-loaded degradable nanoparticles reducing risk of blood coagulation.
- Author
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Reczyńska K, Major R, Kopernik M, Pamuła E, Imbir G, Plutecka H, Bruckert F, and Surmiak M
- Subjects
- Blood Coagulation, Drug Carriers, Eptifibatide, Particle Size, Polylactic Acid-Polyglycolic Acid Copolymer, Polyurethanes, Nanoparticles, Polyglycolic Acid
- Abstract
The main purpose of the work was to develop a drug releasing coatings on the surface of medical devices exposed to blood flow, what should enable effective inhibition of blood coagulation process. As a part of the work, the process of encapsulating the anticoagulant drug eptifibatide (EPT) in poly(DL-lactic-co-glycolic acid) (PLGA) nanoparticles was developed. EPT encapsulation efficiency was 29.1 ± 2.1%, while the EPT loading percentage in the nanoparticles was 4.2 ± 0.3%. The PLGA nanoparticles were suspended in a polyanion solution (hyaluronic acid (HA)) and deposited on the surface-treated thermoplastic polyurethane (TPU) by a layer-by-layer method. As a polycation poly-L-lysine (PLL) was used. The influence of released EPT on the activation of the coagulation system was analyzed using dynamic blood tester. Performed experiments show an effective delivery of the drug to the bloodstream and low risk of platelets (membrane receptor) activation. The dynamic blood test process, including its physical phenomenon, was described using numerical methods, i.e. a finite volume cone-and-plate test model as well as non-Newtonian blood models. The values of shear stress and blood flow velocity under the fast-rotating cone were computed applying boundary conditions of cylinder wall imitating blood-nanomaterial interaction. Implementing boundary conditions as initial shear stress values of bottom cylinder wall resulted in the increase of shear stress in blood under rotating cone. The developed system combining drug eluting polymeric nanoparticles with the polyelectrolyte "layer-by-layer" coating can be easily introduced to medical implants of various shape, with the advantages of resorbable drug carriers allowing for local and controllable delivery of anti-thrombogenic drugs., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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11. Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
- Author
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Grabska-Zielińska S, Sionkowska A, Olewnik-Kruszkowska E, Reczyńska K, and Pamuła E
- Subjects
- Animals, Biocompatible Materials chemistry, Bone and Bones metabolism, Cell Adhesion, Cell Line, Tumor, Collagen chemistry, Cross-Linking Reagents chemistry, Fibroins chemistry, Humans, Microscopy, Electron, Scanning, Osteoblasts drug effects, Porosity, Rats, Regenerative Medicine, Spectroscopy, Fourier Transform Infrared, Tissue Engineering methods, Biopolymers chemistry, Chitosan chemistry, Tissue Scaffolds chemistry
- Abstract
The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm
3 ; cross-linked with DAC: 14.6 ± 0.7 g/cm3 ) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.- Published
- 2021
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12. Response to Comment on "Forest microclimate dynamics drive plant responses to warming".
- Author
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Zellweger F, De Frenne P, Lenoir J, Vangansbeke P, Verheyen K, Bernhardt-Römermann M, Baeten L, Hédl R, Berki I, Brunet J, Van Calster H, Chudomelová M, Decocq G, Dirnböck T, Durak T, Heinken T, Jaroszewicz B, Kopecký M, Máliš F, Macek M, Malicki M, Naaf T, Nagel TA, Ortmann-Ajkai A, Petřík P, Pielech R, Reczyńska K, Schmidt W, Standovár T, Świerkosz K, Teleki B, Vild O, Wulf M, and Coomes D
- Subjects
- Plants, Forests, Microclimate
- Abstract
Schall and Heinrichs question our interpretation that the climatic debt in understory plant communities is locally modulated by canopy buffering. However, our results clearly show that the discrepancy between microclimate warming rates and thermophilization rates is highest in forests where canopy cover was reduced, which suggests that the need for communities to respond to warming is highest in those forests., (Copyright © 2020, American Association for the Advancement of Science.)
- Published
- 2020
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13. Surface-Modified Poly(l-lactide- co -glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects-In Vivo Studies on Rabbits.
- Author
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Krok-Borkowicz M, Reczyńska K, Rumian Ł, Menaszek E, Orzelski M, Malisz P, Silmanowicz P, Dobrzyński P, and Pamuła E
- Subjects
- Animals, Bone Regeneration, Collagen chemistry, Hydroxyapatites chemistry, Porosity, Rabbits, Tissue Scaffolds adverse effects, Osteochondrosis surgery, Polyglactin 910 chemistry, Tissue Scaffolds chemistry
- Abstract
Poly(l-lactide- co -glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250-320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson-Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.
- Published
- 2020
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14. Evaluation of the In Vitro Stability of Stimuli-Sensitive Fatty Acid-Based Microparticles for the Treatment of Lung Cancer.
- Author
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Dałek P, Borowik T, Reczyńska K, Pamuła E, Chrzanowski W, and Langner M
- Subjects
- A549 Cells, Drug Carriers, Humans, Microscopy, Electron, Scanning, Paclitaxel toxicity, Particle Size, Fatty Acids, Lung Neoplasms drug therapy
- Abstract
The fatty acid-based microparticles containing iron oxide nanoparticles and paclitaxel (PAX) are a viable proposition for the treatment of lung cancer. The microparticles inhaled as a dry powder can be guided to selected locations using an external magnetic field, and when accumulated there, the active compound release can be triggered by local hyperthermia. However, this general strategy requires that the active compound is released from microparticles and can reach the targeted cells before microparticles are removed. Isothermal titration calorimetry was used to demonstrate that the components of microparticles were released and transferred to albumins and lipid bilayers. The morphology of the measured particulates was studied with scanning electron microscopy and dynamic light scattering. To determine the cytotoxicity of microparticles, cell culture studies were done. It has been shown that the transfer efficiency depends predominantly on the fatty acid composition of microparticles, which, together with the active ingredient, accumulate predominantly in membrane structures after being released from microparticles and before entering the cytoplasm. The release process is sufficient; hence, paclitaxel-loaded microparticles effectively suppressed the proliferation of A549 human lung epithelial cells of malignant origin (IC
50 values for both lauric acid-based and myristic/palmitic-based microparticles containing paclitaxel were below 0.375 μg/mL), while reference microparticles were noncytotoxic.- Published
- 2020
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15. Replacements of small- by large-ranged species scale up to diversity loss in Europe's temperate forest biome.
- Author
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Staude IR, Waller DM, Bernhardt-Römermann M, Bjorkman AD, Brunet J, De Frenne P, Hédl R, Jandt U, Lenoir J, Máliš F, Verheyen K, Wulf M, Pereira HM, Vangansbeke P, Ortmann-Ajkai A, Pielech R, Berki I, Chudomelová M, Decocq G, Dirnböck T, Durak T, Heinken T, Jaroszewicz B, Kopecký M, Macek M, Malicki M, Naaf T, Nagel TA, Petřík P, Reczyńska K, Schei FH, Schmidt W, Standovár T, Świerkosz K, Teleki B, Van Calster H, Vild O, and Baeten L
- Subjects
- Biodiversity, Europe, Plants, Ecosystem, Forests
- Abstract
Biodiversity time series reveal global losses and accelerated redistributions of species, but no net loss in local species richness. To better understand how these patterns are linked, we quantify how individual species trajectories scale up to diversity changes using data from 68 vegetation resurvey studies of seminatural forests in Europe. Herb-layer species with small geographic ranges are being replaced by more widely distributed species, and our results suggest that this is due less to species abundances than to species nitrogen niches. Nitrogen deposition accelerates the extinctions of small-ranged, nitrogen-efficient plants and colonization by broadly distributed, nitrogen-demanding plants (including non-natives). Despite no net change in species richness at the spatial scale of a study site, the losses of small-ranged species reduce biome-scale (gamma) diversity. These results provide one mechanism to explain the directional replacement of small-ranged species within sites and thus explain patterns of biodiversity change across spatial scales.
- Published
- 2020
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16. Sodium alendronate loaded poly(l-lactide- co -glycolide) microparticles immobilized on ceramic scaffolds for local treatment of bone defects.
- Author
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Rumian Ł, Wolf-Brandstetter C, Rößler S, Reczyńska K, Tiainen H, Haugen HJ, Scharnweber D, and Pamuła E
- Abstract
Bone tissue regeneration in critical-size defects is possible after implantation of a 3D scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing. Sodium alendronate (Aln), a widely used anti-osteoporosis drug, exhibits strong inhibitory effect on bone resorption performed by osteoclasts. Thus, we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide scaffolds and poly(l-lactide- co -glycolide) microparticles (MPs) loaded with Aln. The MPs were effectively attached to the surface of the scaffolds' pore walls by human recombinant collagen. Drug release from the scaffolds was characterized by initial burst (24 ± 6% of the drug released within first 24 h) followed by a sustained release phase (on average 5 µg of Aln released per day from Day 3 to Day 18). In vitro tests evidenced that Aln at concentrations of 5 and 2.5 µg/ml was not cytotoxic for MG-63 osteoblast-like cells (viability between 81 ± 6% and 98 ± 3% of control), but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells, as shown by reduced fusion capability and decreased tartrate-resistant acid phosphatase 5b activity (56 ± 5% reduction in comparison to control after 8 days of culture). Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis, reducing osteoclast activity, but not affecting osteoblast functions, which may be beneficial in the treatment of critical-size bone tissue defects., (© The Author(s) 2020. Published by Oxford University Press.)
- Published
- 2020
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17. Stimuli-sensitive fatty acid-based microparticles for the treatment of lung cancer.
- Author
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Reczyńska K, Marchwica P, Khanal D, Borowik T, Langner M, Pamuła E, and Chrzanowski W
- Subjects
- A549 Cells, Electric Impedance, Humans, Magnetic Phenomena, Particle Size, Temperature, Cell-Derived Microparticles metabolism, Fatty Acids therapeutic use, Lung Neoplasms therapy
- Abstract
Despite recent advancements in medicine, lung cancer still lacks an effective therapy. In the present study we have decided to combine superparamagnetic iron oxide nanoparticles (SPION) with solid lipid microparticles to develop novel, stimuli-sensitive drug carriers that increase the bioavailability of the anticancer drug (paclitaxel - PAX) through guided accumulation directly at the tumour site and controlled drug delivery. SPION and PAX-loaded microparticles (MPs) were fabricated from lauric acid (LAU) and a mixture of myristic and palmitic acids (MYR/PAL) using hot oil-in-water emulsification method. MP size, surface properties, melting temperature and magnetic mobility were evaluated along with their in vitro efficacy against malignant lung epithelial cells (A549). MPs were spherical in shape with the average particle size between 2 and 3.5 μm and responded to external magnetic field up to the distance of 15 mm. MPs were effectively internalised by the cells. Unloaded or NP-loaded MPs were cytocompatible with A549 cells, while NP + PAX-loaded MPs significantly decreased cell viability and effectively suppressed colony formation. The developed stimuli-sensitive, inhalable MPs have shown promising results as PAX carriers for controlled pulmonary delivery for the treatment of lung cancer., Competing Interests: Declaration of competing interest There are no conflicts to declare., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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18. Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment.
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Reczyńska K, Marszałek M, Zarzycki A, Reczyński W, Kornaus K, Pamuła E, and Chrzanowski W
- Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) are promising drug delivery carriers and hyperthermia agents for the treatment of cancer. However, to ensure their safety in vivo, SPIONs must be modified in order to prevent unwanted iron release. Thus, SPIONs were coated with silica layers of different morphologies: non-porous (@SiO
2 ), mesoporous (@mSiO2 ) or with a combination of non-porous and mesoporous layers (@SiO2 @mSiO2 ) deposited via a sol-gel method. The presence of SiO2 drastically changed the surface properties of the nanoparticles. The zeta potential changed from 19.6 ± 0.8 mV for SPIONs to -26.1 ± 0.1 mV for SPION@mSiO2 . The Brunauer-Emmett-Teller (BET) surface area increased from 7.54 ± 0.02 m2 /g for SPIONs to 101.3 ± 2.8 m2 /g for SPION@mSiO2 . All types of coatings significantly decreased iron release (at least 10 fold as compared to unmodified SPIONs). SPIONs and SPION@mSiO2 were tested in vitro in contact with human lung epithelial cells (A549 and BEAS-2B). Both nanoparticle types were cytocompatible, although some delay in proliferation was observed for BEAS-2B cells as compared to A549 cells, which was correlated with increased cell velocity and nanoparticles uptake.- Published
- 2020
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19. Forest microclimate dynamics drive plant responses to warming.
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Zellweger F, De Frenne P, Lenoir J, Vangansbeke P, Verheyen K, Bernhardt-Römermann M, Baeten L, Hédl R, Berki I, Brunet J, Van Calster H, Chudomelová M, Decocq G, Dirnböck T, Durak T, Heinken T, Jaroszewicz B, Kopecký M, Máliš F, Macek M, Malicki M, Naaf T, Nagel TA, Ortmann-Ajkai A, Petřík P, Pielech R, Reczyńska K, Schmidt W, Standovár T, Świerkosz K, Teleki B, Vild O, Wulf M, and Coomes D
- Subjects
- Europe, Forests, Global Warming, Microclimate, Trees physiology
- Abstract
Climate warming is causing a shift in biological communities in favor of warm-affinity species (i.e., thermophilization). Species responses often lag behind climate warming, but the reasons for such lags remain largely unknown. Here, we analyzed multidecadal understory microclimate dynamics in European forests and show that thermophilization and the climatic lag in forest plant communities are primarily controlled by microclimate. Increasing tree canopy cover reduces warming rates inside forests, but loss of canopy cover leads to increased local heat that exacerbates the disequilibrium between community responses and climate change. Reciprocal effects between plants and microclimates are key to understanding the response of forest biodiversity and functioning to climate and land-use changes., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Published
- 2020
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20. Distinct Influence of Saturated Fatty Acids on Malignant and Nonmalignant Human Lung Epithelial Cells.
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Reczyńska K, Khanal D, Pielichowska K, Pamuła E, and Chrzanowski W
- Subjects
- A549 Cells, Cell Membrane drug effects, Cell Proliferation drug effects, Cell Survival drug effects, Elastic Modulus drug effects, Humans, Lung Neoplasms drug therapy, Fatty Acids pharmacology, Lung Neoplasms metabolism
- Abstract
The impact of saturated fatty acids (FA) on viability and properties of malignant and nonmalignant cells has not been studied in detail so far. The present study was aimed at evaluation of the influence of saturated FA (10:0-18:0) on malignant (A459) and nonmalignant (BEAS-2B) human lung epithelial cells. FA strongly affected A549 cells, but not BEAS-2B cells. Viability of A549 cells incubated with 14:0-18:0 was decreased by 53-91% as compared to untreated cells. Cell membrane stiffness in those cells as measured by atomic force microscopy was also reduced. Median value of apparent Young's modulus of untreated A549 cell membrane was 16.9 kPa and it decreased to 8.9 kPa for cells incubated with 14:0. Viability and mechanical properties of BEAS-2B cells were not altered by presence of FA. Those surprising discrepancies can be related to the differences in FA uptake rate. A549 cells were found to incorporate higher amount of FA and this corresponded to decrease in cell membrane stiffness and reduced cell viability. The performed studies showed that saturated FA have distinct influence on various types of cells, which may be exploited in development of the advanced lipid drug delivery systems., (© 2020 AOCS.)
- Published
- 2020
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21. Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch.
- Author
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Grabska-Zielińska S, Sionkowska A, Reczyńska K, and Pamuła E
- Abstract
In this study, three-dimensional (3D) biopolymeric scaffolds made from collagen, silk fibroin and chitosan were successfully prepared by the freeze drying method. Dialdehyde starch (DAS) was used as a cross-linking agent for the materials. The properties of the materials were studied using density and porosity measurements, scanning electron microscope (SEM) imaging, swelling and moisture content measurements. Additionally, cytocompatibility of the materials in contact with MG-63 osteoblast-like cells was tested by live/dead staining and resazurin reduction assay on days 1, 3 and 7. It was found that new 3D materials made from collagen/silk fibroin/chitosan binary or ternary mixtures are hydrophilic with a high swelling ability (swelling rate in the range of 1680-1900%). Cross-linking of such biopolymeric materials with DAS increased swelling rate up to about 2100%, reduced porosity from 96-97% to 91-93%, and also decreased density and moisture content of the materials. Interestingly, presence of DAS did not influence the microstructure of the scaffolds as compared to non-cross-linked samples as shown by SEM. All the tested samples were found to be cytocompatible and supported adhesion and growth of MG-63 cells as shown by live-dead staining and metabolic activity test.
- Published
- 2020
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22. Understanding context dependency in the response of forest understorey plant communities to nitrogen deposition.
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Perring MP, Diekmann M, Midolo G, Schellenberger Costa D, Bernhardt-Römermann M, Otto JCJ, Gilliam FS, Hedwall PO, Nordin A, Dirnböck T, Simkin SM, Máliš F, Blondeel H, Brunet J, Chudomelová M, Durak T, De Frenne P, Hédl R, Kopecký M, Landuyt D, Li D, Manning P, Petřík P, Reczyńska K, Schmidt W, Standovár T, Świerkosz K, Vild O, Waller DM, and Verheyen K
- Subjects
- Biodiversity, Ecosystem, Nitrogen Cycle, Plants, Soil, Trees growth & development, Forests, Nitrogen analysis
- Abstract
Understorey communities can dominate forest plant diversity and strongly affect forest ecosystem structure and function. Understoreys often respond sensitively but inconsistently to drivers of ecological change, including nitrogen (N) deposition. Nitrogen deposition effects, reflected in the concept of critical loads, vary greatly not only among species and guilds, but also among forest types. Here, we characterize such context dependency as driven by differences in the amounts and forms of deposited N, cumulative deposition, the filtering of N by overstoreys, and available plant species pools. Nitrogen effects on understorey trajectories can also vary due to differences in surrounding landscape conditions; ambient browsing pressure; soils and geology; other environmental factors controlling plant growth; and, historical and current disturbance/management regimes. The number of these factors and their potentially complex interactions complicate our efforts to make simple predictions about how N deposition affects forest understoreys. We review the literature to examine evidence for context dependency in N deposition effects on forest understoreys. We also use data from 1814 European temperate forest plots to test the ability of multi-level models to characterize context-dependent understorey responses across sites that differ in levels of N deposition, community composition, local conditions and management history. This analysis demonstrated that historical management, and plot location on light and pH-fertility gradients, significantly affect how understorey communities respond to N deposition. We conclude that species' and communities' responses to N deposition, and thus the determination of critical loads, vary greatly depending on environmental contexts. This complicates our efforts to predict how N deposition will affect forest understoreys and thus how best to conserve and restore understorey biodiversity. To reduce uncertainty and incorporate context dependency in critical load setting, we should assemble data on underlying environmental conditions, conduct globally distributed field experiments, and analyse a wider range of habitat types., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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23. Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.
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Lopez-Heredia MA, Łapa A, Reczyńska K, Pietryga K, Balcaen L, Mendes AC, Schaubroeck D, Van Der Voort P, Dokupil A, Plis A, Stevens CV, Parakhonskiy BV, Samal SK, Vanhaecke F, Chai F, Chronakis IS, Blanchemain N, Pamuła E, Skirtach AG, and Douglas TEL
- Subjects
- Animals, Cell Line, Cell Proliferation drug effects, Hydrogels pharmacology, Materials Testing, Mice, Osteoblasts metabolism, Polysaccharides, Bacterial pharmacology, Calcium Carbonate chemistry, Hydrogels chemistry, Magnesium chemistry, Polysaccharides, Bacterial chemistry
- Abstract
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO
3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration. In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution. This alternate soaking cycle was repeated five times. Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared. Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle. Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution. Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite. Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity. Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days., (© 2018 John Wiley & Sons, Ltd.)- Published
- 2018
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24. Global environmental change effects on plant community composition trajectories depend upon management legacies.
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Perring MP, Bernhardt-Römermann M, Baeten L, Midolo G, Blondeel H, Depauw L, Landuyt D, Maes SL, De Lombaerde E, Carón MM, Vellend M, Brunet J, Chudomelová M, Decocq G, Diekmann M, Dirnböck T, Dörfler I, Durak T, De Frenne P, Gilliam FS, Hédl R, Heinken T, Hommel P, Jaroszewicz B, Kirby KJ, Kopecký M, Lenoir J, Li D, Máliš F, Mitchell FJG, Naaf T, Newman M, Petřík P, Reczyńska K, Schmidt W, Standovár T, Świerkosz K, Van Calster H, Vild O, Wagner ER, Wulf M, and Verheyen K
- Subjects
- Climate, Europe, Forests, Human Activities, Nitrogen, Biodiversity, Plants classification
- Abstract
The contemporary state of functional traits and species richness in plant communities depends on legacy effects of past disturbances. Whether temporal responses of community properties to current environmental changes are altered by such legacies is, however, unknown. We expect global environmental changes to interact with land-use legacies given different community trajectories initiated by prior management, and subsequent responses to altered resources and conditions. We tested this expectation for species richness and functional traits using 1814 survey-resurvey plot pairs of understorey communities from 40 European temperate forest datasets, syntheses of management transitions since the year 1800, and a trait database. We also examined how plant community indicators of resources and conditions changed in response to management legacies and environmental change. Community trajectories were clearly influenced by interactions between management legacies from over 200 years ago and environmental change. Importantly, higher rates of nitrogen deposition led to increased species richness and plant height in forests managed less intensively in 1800 (i.e., high forests), and to decreases in forests with a more intensive historical management in 1800 (i.e., coppiced forests). There was evidence that these declines in community variables in formerly coppiced forests were ameliorated by increased rates of temperature change between surveys. Responses were generally apparent regardless of sites' contemporary management classifications, although sometimes the management transition itself, rather than historic or contemporary management types, better explained understorey responses. Main effects of environmental change were rare, although higher rates of precipitation change increased plant height, accompanied by increases in fertility indicator values. Analysis of indicator values suggested the importance of directly characterising resources and conditions to better understand legacy and environmental change effects. Accounting for legacies of past disturbance can reconcile contradictory literature results and appears crucial to anticipating future responses to global environmental change., (© 2017 John Wiley & Sons Ltd.)
- Published
- 2018
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- View/download PDF
25. Animal models of smoke inhalation injury and related acute and chronic lung diseases.
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Reczyńska K, Tharkar P, Kim SY, Wang Y, Pamuła E, Chan HK, and Chrzanowski W
- Subjects
- Animals, Disease Models, Animal, Lung Diseases pathology, Smoke Inhalation Injury pathology
- Abstract
Smoke inhalation injury leads to various acute and chronic lung diseases and thus is the dominant cause of fire-related fatalities. In a search for an effective treatment and validation of therapies different classes of animal models have been developed, which include both small and large animals. These models have advanced our understanding of the mechanism of smoke inhalation injury, enabling a better understanding of pathogenesis and pathophysiology and development of new therapies. However, none of the animal models fully mirrors human lungs and their pathologies. All animal models have their limitations in replicating complex clinical conditions associated with smoke inhalation injury in humans. Therefore, for a correct interpretation of the results and to avoid bias, a precise understanding of similarities and differences of lungs between different animal species and humans is critical. We have reviewed and presented comprehensive comparison of different animal models and their clinical relevance. We presented an overview of methods utilized to induce smoke inhalation injuries, airway micro-/macrostructure, advantages and disadvantages of the most commonly used small and large animal models., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2018
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26. Ca:Mg:Zn:CO 3 and Ca:Mg:CO 3 -tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
- Author
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Douglas TEL, Sobczyk K, Łapa A, Włodarczyk K, Brackman G, Vidiasheva I, Reczyńska K, Pietryga K, Schaubroeck D, Bliznuk V, Voort PV, Declercq HA, Bulcke JVD, Samal SK, Khalenkow D, Parakhonskiy BV, Van Acker J, Coenye T, Lewandowska-Szumieł M, Pamuła E, and Skirtach AG
- Subjects
- 3T3 Cells, Animals, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents chemistry, Biocompatible Materials administration & dosage, Calcium Carbonate chemistry, Hydrogels chemistry, Injections, Magnesium chemistry, Materials Testing, Methicillin-Resistant Staphylococcus aureus drug effects, Mice, Microscopy, Electron, Osteoblasts cytology, Particle Size, Polysaccharides, Bacterial chemistry, Rheology, X-Ray Diffraction, Zinc Compounds chemistry, Biocompatible Materials chemistry, Carbonates chemistry, Regeneration, Tissue Engineering methods
- Abstract
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg. Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg. Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition. Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites. Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus. Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial. This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
- Published
- 2017
- Full Text
- View/download PDF
27. Combining community resurvey data to advance global change research.
- Author
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Verheyen K, De Frenne P, Baeten L, Waller DM, Hédl R, Perring MP, Blondeel H, Brunet J, Chudomelova M, Decocq G, De Lombaerde E, Depauw L, Dirnböck T, Durak T, Eriksson O, Gilliam FS, Heinken T, Heinrichs S, Hermy M, Jaroszewicz B, Jenkins MA, Johnson SE, Kirby KJ, Kopecký M, Landuyt D, Lenoir J, Li D, Macek M, Maes S, Máliš F, Mitchell FJG, Naaf T, Peterken G, Petřík P, Reczyńska K, Rogers DA, Schei FH, Schmidt W, Standovár T, Świerkosz K, Ujházy K, Van Calster H, Vellend M, Vild O, Woods K, Wulf M, and Bernhard-Römermann M
- Abstract
More and more ecologists have started to resurvey communities sampled in earlier decades to determine long-term shifts in community composition and infer the likely drivers of the ecological changes observed. However, to assess the relative importance of, and interactions among, multiple drivers joint analyses of resurvey data from many regions spanning large environmental gradients are needed. In this paper we illustrate how combining resurvey data from multiple regions can increase the likelihood of driver-orthogonality within the design and show that repeatedly surveying across multiple regions provides higher representativeness and comprehensiveness, allowing us to answer more completely a broader range of questions. We provide general guidelines to aid implementation of multi-region resurvey databases. In so doing, we aim to encourage resurvey database development across other community types and biomes to advance global environmental change research., Competing Interests: None of the authors has a conflict of interest.
- Published
- 2016
- Full Text
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28. Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
- Author
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Douglas TE, Łapa A, Reczyńska K, Krok-Borkowicz M, Pietryga K, Samal SK, Declercq HA, Schaubroeck D, Boone M, Van der Voort P, De Schamphelaere K, Stevens CV, Bliznuk V, Balcaen L, Parakhonskiy BV, Vanhaecke F, Cnudde V, Pamuła E, and Skirtach AG
- Subjects
- Biocompatible Materials chemistry, Calcium Carbonate chemistry, Cell Culture Techniques, Cell Line, Tumor, Humans, Ions, Materials Testing, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, X-Ray Microtomography, Bone Regeneration, Calcium chemistry, Hydrogels chemistry, Magnesium chemistry, Polysaccharides, Bacterial chemistry
- Abstract
The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability. In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca
2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites. It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation. The effect of Mg incorporation on cytocompatibility and cell growth was also studied. Microparticles were formed by mixing Ca2+ and Mg2+ and [Formula: see text] ions in varying concentrations. Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites. The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added. In the absence of Mg, vaterite was formed. At low Mg content, magnesian calcite was formed. Increasing the Mg content further caused formation of amorphous mineral. Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min. Microparticles were dispersed homogeneously in hydrogels. MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves. All composites were cytocompatible. Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio. In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.- Published
- 2016
- Full Text
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29. Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization.
- Author
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Douglas TE, Dokupil A, Reczyńska K, Brackman G, Krok-Borkowicz M, Keppler JK, Božič M, Van Der Voort P, Pietryga K, Samal SK, Balcaen L, van den Bulcke J, Van Acker J, Vanhaecke F, Schwarz K, Coenye T, and Pamuła E
- Subjects
- Alkaline Phosphatase metabolism, Anti-Infective Agents chemistry, Biocompatible Materials chemistry, Bone Regeneration, Calcium Phosphates chemistry, Cell Line, Humans, Methicillin-Resistant Staphylococcus aureus, Microbial Sensitivity Tests, Osteoblasts cytology, Solubility, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Thermogravimetry, Anti-Bacterial Agents chemistry, Hydrogels chemistry, Polysaccharides, Bacterial chemistry, Seaweed chemistry, Tannins chemistry
- Abstract
Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol(®), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol(®) enhanced mineralization with CaP and also increased compressive modulus. Seanol(®) and ALP interacted in a non-covalent manner. Release of Seanol(®) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.
- Published
- 2016
- Full Text
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30. The influence of sintering conditions on microstructure and mechanical properties of titanium dioxide scaffolds for the treatment of bone tissue defects.
- Author
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Rumian Ł, Reczyńska K, Wrona M, Tiainen H, Haugen HJ, and Pamuła E
- Subjects
- Bone Regeneration, Bone and Bones pathology, Ceramics chemistry, Compressive Strength, Hot Temperature, Humans, Materials Testing, Microscopy, Electron, Scanning, Polymers chemistry, Polyurethanes chemistry, Porosity, Powders, Stress, Mechanical, Tissue Engineering methods, X-Ray Microtomography, Bone and Bones drug effects, Tissue Engineering instrumentation, Tissue Scaffolds chemistry, Titanium chemistry
- Abstract
In this study the attempts to improve mechanical properties of highly-porous titanium dioxide scaffolds produced by polymer sponge replication method were investigated. Particularly the effect of two-step sintering at different temperatures on microstructure and mechanical properties (compression test) of the scaffolds were analysed. To this end microcomputed tomography and scanning electron microscopy were used as analytical methods. Our experiments showed that the most appropriate conditions of manufacturing were when the scaffolds were heat-treated at 1500 °C for 1 h followed by sintering at 1200 °C for 20 h. Such scaffolds exhibited the highest compressive strength which was correlated with the highest linear density and the lowest size of grains. Moreover, grain size distribution was narrower with predominating fraction of fine grains 10-20 μm in size. Smaller grains and higher linear density sug- gested that in this case densification process prevailed over undesirable process of grain coarsening, which finally resulted in im- proved mechanical properties of the scaffolds.
- Published
- 2015
31. Methodology and evaluation of the renal arterial system.
- Author
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Drewniak T, Rzepecki M, Juszczak K, Moczulski Z, Reczyńska K, and Jakubowska M
- Abstract
Introduction: The broad range of medical images and image processing technologies are applied in urology. The aim was to propose methodology to assess three-dimensional (3D) arrangement of renal arterial tree and to build a statistical model for analyzing the layout of arteries in the sections of the kidney., Methods: The series of kidney CT slices are analyzed using image processing procedures and further the 3D model of arterial systems is converted to a graph tree which includes information about features of the renal arterial system., Results: The selected endocast was transformed to the form of the 3D connected tubes, further to the tree data structure and next analyzed. The information about 3D coordinates of the nodes, also branch length and diameter were stored. Renal arterial system of the considered kidney possessed 181 branches with 14 bifurcation levels. The number of branches was highest at the 9th bifurcation level. The mean length of the arterial branch on each bifurcation level was constant (6 mm). The branch diameters rapidly decreased after each bifurcation. The number of terminal branches increases up to 9th level where there are 19 terminal branches. The mean length of terminal arteries was 7.17 mm while the mean radius 0.46 mm. A statistically significant correlation between parameters that described sub-trees was noticed. It was observed that the individual artery segments occupy a separate space in the kidney volume., Conclusions: The methodology has the potential to assist in presurgical planning based on branching patterns of the renal arterial system and corresponding pathology.
- Published
- 2013
- Full Text
- View/download PDF
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