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ABSTRACT This study aimed to evaluate the in vitro gas production from three pearl millet cultivars submitted to four nitrogen levels. The treatments consisted of three pearl millet cultivars (ADR 500, ADR 7020, and LABH 70732) and four nitrogen levels (0, 50, 100, and 200 kg/ha), using urea as the nitrogen source. A 3 x 4 factorial experimental design, employing a randomized block setup, was used to assess both gas production and digestibility. No interaction was observed between nitrogen doses and fermentative parameters on in vitro gas production from the dry matter of pearl millet cultivars. Nitrogen had no impact on the dry matter content or chemical composition of the cultivars. Consistency prevailed in the chemical composition across the various cultivars, a trend that extended to the gas production rates for both soluble and insoluble contents. Notably, nitrogen fertilizer exerted an influence on the fermentation pattern of organic matter, gas production, and lag time for the cultivars. Nitrogen fertilization is advised solely for enhancing fermentative attributes in the case of the cultivar ADR 7020.

It is important to evaluate nutritional value of new grass species, which can be better characterized through rumen fermentation and degradation techniques. The aim was to evaluate the effects of Nitrogen (N) fertilization on the kinetics of fermentation and ruminal degradation of the Mulato II grass. Two distinct sources of N (common and protected urea) were used during two periods of the year (summer - I and autumn - II). A completely randomized experimental design was used, in a 2x4 factorial arrangement with three replicates. Fraction A changed based on N dosage. The passage rate (K) and effective degradability (ED) were influenced by sources and doses of N, while Fraction B and Lag Time were not influenced during period I. Fractions A and B and EDs were all influenced by N sources and doses during period II. Lag time and potential degradability were influenced by N sources only. Among the kinetics parameters of dry matter ruminal fermentation obtained during period I, only K1 was influenced by the interaction between N doses x sources, while the remaining parameters were only influenced by the N doses applied. As for period II, with the exception of K2, all remaining parameters were influenced by the interaction of N doses and sources. Protected urea improved fermentation parameters during period I. The dose equivalent to applying 150kg/ha positively influenced ruminal fermentation parameters of the Mulato II grass mainly in the summer. The use of protected urea as a Nitrogen source improved fermentation parameters in the summer. Keywords: Brachiaria; digestibility; digestion kinetics; rumen fermentation; gas production

Foram avaliados a composição morfológica, os parâmetros agronômicos, a produção de massa seca das plantas forrageiras utilizadas para o processo de ensilagem, bem como a caracterização do processo fermentativo, em diferentes tempos de abertura dos mini-silos (3, 7, 15, 30, 60, 120 dias) após a ensilagem. Procedeu-se também às determinações das perdas de efluentes e gases, além da composição bromatológica e fracionamento de proteína das silagens. Também foi estimado o custo de produção e a produção de leite em função da matéria seca das silagens produzidas a partir de diferentes gramíneas. As espécies forrageiras avaliadas foram: milheto ADR-7010, sorgo BRS 610, milho AG 5055 com espiga, milho AG 5055 sem espiga e cana-de-açúcar IAC 86-2480. Foram observadas diferenças significativas tanto para produção, quanto composição bromatológica e custos de produção. O milheto não se mostrou competitivo com as demais forrageiras; entretanto, constitui opção como cultura de inverno em decorrência de sua baixa exigência hídrica quando comparado às demais culturas.