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Racism has been declared a public health threat. With increased direct and vicarious exposure to racism-based violence through social media, we explored the associations between racism-based events and traumatic stress symptomatology, as well as self- and collective care (inclusive of coping, activism, and ethnic and racial identity) through a mixed-methods approach. A total of 104 racism-based events were reported across 43 Black and/or Latine/x emerging adults in the sample, with a majority endorsing racism-based stress or traumatic stress (i.e., the symptomatology associated with a racism-based event). Individuals who reported higher racism-based traumatic stress symptoms immediately following a racism-based event also reported higher ethnic identity resolution scores. Further, 19%–42% of participants reported racism-based traumatic stress and racism-based stress more recently (respectively), showing that racism-based events may be traumatic stressors with long-term mental health consequences. Participants provided thick descriptions of how they defined and engaged in self- and collective care as wellness and activism and reported engaging in cultural, ancestral, spiritual, and religious practices in an attempt to heal. The findings of this study underscore the importance of radical hope and radical healing for Black and Latine/x communities. Public Policy Relevance Statement: Exposure to racism, whether directly or vicariously, has negative implications for mental health; while this has been well-documented, traumatic stress has not been included in mental health diagnostic codes. This study suggests that mental health policies at the institutional and structural level should address direct and vicarious exposure to racism as well as acknowledge these experiences as bona fide traumatic stressors necessitating their own diagnostic category or code. Policies must also be created to address the harm done to communities of color and improve clinician training to affirm and support racially and ethnically minoritized individuals in their self-healing and collective healing journey and pursuit for racial justice. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To evaluate the relation between household food insecurity (HFI) and fear of hypoglycemia among young adults with type 1 and type 2 diabetes and adolescents with type 1 diabetes and their parents. RESEARCH DESIGN AND METHODS: We analyzed cross-sectional data of 1,676 young adults with youth-onset diabetes (84% type 1, 16% type 2) and 568 adolescents (<18 years old; mean age 15.1 years) with type 1 diabetes from the SEARCH for Diabetes in Youth study. Adult participants and parents of adolescent participants completed the U.S. Household Food Security Survey Module. Adults, adolescents, and parents of adolescents completed the Hypoglycemia Fear Survey, where answers range from 1 to 4. The outcomes were mean score for fear of hypoglycemia and the behavior and worry subscale scores. Linear regression models identified associations between HFI and fear of hypoglycemia scores. RESULTS: Adults with type 1 diabetes experiencing HFI had higher fear of hypoglycemia scores (0.22 units higher for behavior, 0.55 units for worry, 0.40 units for total; all P < 0.0001) than those without HFI. No differences by HFI status were found for adolescents with type 1 diabetes. Parents of adolescents reporting HFI had a 0.18 unit higher worry score than those not reporting HFI (P < 0.05). Adults with type 2 diabetes experiencing HFI had higher fear of hypoglycemia scores (0.19 units higher for behavior, 0.35 units for worry, 0.28 units for total; all P < 0.05) than those in food secure households. CONCLUSIONS: Screening for HFI and fear of hypoglycemia among people with diabetes can help providers tailor diabetes education for those who have HFI and therefore fear hypoglycemia. [ABSTRACT FROM AUTHOR]

Objective: To examine the association between household food insecurity (HFI), glycemic control, severe hypoglycemia and diabetic ketoacidosis (DKA) among youth and young adults (YYA) with youth‐onset type 2 diabetes. Research design and methods: This cross‐sectional study included 395 YYA with type 2 diabetes from the SEARCH for Diabetes in Youth Study (2015–2019). HFI was reported by young adult participants or parents of minor participants via the US Household Food Security Survey Module. Glycemic control was assessed by HbA1c and analyzed as a continuous and categorical variable (optimal: <7.0%, suboptimal: ≥7.0%–9.0%, poor: >9.0%). Acute complications included self‐reported severe hypoglycemia or DKA in the last 12 months. Adjusted logistic and linear regression were used for binary and continuous outcomes, respectively. Results: Approximately 31% reported HFI in the past 12 months. Mean HbA1c among those with HFI was 9.2% compared to 9.5% without HFI. Of those with HFI, 56% had an HbA1c >9.0% compared to 55% without HFI. Adjusted models showed no associations between HFI and glycemic control. Of those with HFI, 14.4% reported experiencing DKA and 4.7% reported severe hypoglycemia. YYA with HFI had 3.08 times (95% CI: 1.18–8.06) the odds of experiencing DKA as those without HFI. There was no association between HFI and severe hypoglycemia. Conclusions: HFI was associated with markedly increased odds of DKA but not with glycemic control or severe hypoglycemia. Future research among YYA with type 2 diabetes should evaluate longitudinally whether alleviating HFI reduces DKA. [ABSTRACT FROM AUTHOR]

• CPPs can partake in multiple cellular uptake mechanisms. • MD simulations can be used to gain atomistic insight into CPP uptake mechanisms. • Biomolecular MD simulations are challenging owing to complex energy landscapes. • Enhanced sampling methods can increase sampling of the energy landscape. Cell-penetrating peptides (CPPs) offer an exciting approach to tackle the pharmacokinetic challenges associated with the delivery of large, polar molecules to intracellular targets. Since the discovery of the first CPPs in the early 1990s, vast amounts of research have been undertaken to characterise their cellular uptake mechanisms. Despite this, the precise mechanisms of cellular internalisation of many CPPs remain elusive owing to inconsistent experimental results. Molecular dynamics (MD) simulations provide an approach to probe specific aspects of the internalisation process and many published CPP studies have incorporated simulation data. This review provides a critical evaluation of the current approaches that are being used to simulate CPPs interacting with artificial lipid bilayers. This review discusses the simulation techniques currently being used to study cell-penetrating peptides, along with the mechanistic insights gained and challenges associated with such studies. [ABSTRACT FROM AUTHOR]

Household food insecurity is associated with poor dietary intake in the general population, but little is known about this association in persons with diabetes. We examined the degree of adherence to the dietary reference intakes and 2020-2025 Dietary Guidelines for Americans overall and according to food security status and diabetes type among youth and young adults (YYA) with youth-onset diabetes. The SEARCH for Diabetes in Youth study includes 1,197 YYA with type 1 diabetes (mean age = 21 ± 5 years) and 319 YYA with type 2 diabetes (25 ± 4 years). Participants (or parents if younger than age 18 years) completed the US Department of Agriculture Household Food Security Survey Module, wherein ≥3 affirmations indicate food insecurity. Diet was assessed via food frequency questionnaire and compared with age- and sex-specific dietary reference intakes for 10 nutrients and dietary components (calcium; fiber; magnesium; potassium; sodium; vitamins C, D, and E; added sugar; and saturated fat). Median regression models controlled for sex- and type-specific means for age, diabetes duration, and daily energy intake. Prevalence of guideline adherence was overarchingly poor, with <40% of participants meeting recommendations for eight of 10 nutrients and dietary components; however, higher adherence (>47%) was observed for vitamin C and added sugars. YYA with type 1 diabetes who were food insecure were more likely to meet recommendations for calcium, magnesium, and vitamin E (P < 0.05), and less likely for sodium (P < 0.05) than those with food security. In adjusted models, YYA with type 1 diabetes who were food secure had closer median adherence to sodium (P = 0.002) and fiber (P = 0.042) guidelines than those food insecure. No associations were observed in YYA with type 2 diabetes. Food insecurity is associated with lesser adherence to fiber and sodium guidelines in YYA with type 1 diabetes, which may lead to diabetes complications and other chronic diseases. [ABSTRACT FROM AUTHOR]

Background: Organic nitrates uncouple bone turnover, improve bone mineral density, and improve trabecular and cortical components of bone. These changes in turnover, strength and geometry may translate into an important reduction in fractures. However, before proceeding with a large fracture trial, there is a need to identify the nitrate formulation that has both the greatest efficacy (with regards to bone turnover markers) and gives the fewest headaches. Ascertaining which nitrate formulation this may be is the purpose of the current study. Methods and design: This will be an open-label randomized, controlled trial conducted at Women's College Hospital comparing five formulations of nitrates for their effects on bone turnover markers and headache. We will recruit postmenopausal women age 50 years or older with no contraindications to nitroglycerin. Our trial will consist of a run-in phase and a treatment phase. We will enroll 420 women in the run-in phase, each to receive all of the 5 potential treatments in random order for 2 days, each with a 2-day washout period between treatments. Those who tolerate all formulations will enter the 12-week treatment phase and be randomly assigned to one of five groups: 0.3 mg sublingual nitroglycerin tablet, 0.6 mg of the sublingual tablet, a 20 mg tablet of isosorbide mononitrate, a 160 mg nitroglycerin transdermal patch (used for 8 h), and 15 mg of nitroglycerin ointment as used in a previous trial by our group. We will continue enrolment until we have randomized 210 women or 35 women per group. Concentrations of bone formation (bone-specific alkaline phosphatase and procollagen type I N-terminal propeptide) and bone resorption (C-telopeptides of collagen crosslinks and N-terminal crosslinks of collagen) agents will be measured in samples taken at study entry (the start of the run in phase) and 12 weeks. Subjects will record the frequency and severity of headaches daily during the run-in phase and then monthly after that. We will use the 'multiple comparisons with the best' approach for data analyses, as this strategy allows practical considerations of ease of use and tolerability to guide selection of the preparation for future studies. Discussion: Data from this protocol will be used to develop a randomized, controlled trial of nitrates to prevent osteoporotic fractures. [ABSTRACT FROM AUTHOR]

Household food insecurity is associated with poor dietary intake in the general population, but little is known about persons with diabetes. We examined dietary intake among youth and young adults (YYA) with diabetes in relation to food security (FS) to identify differences in intake quantity and adherence to recommendations. The SEARCH for Diabetes in Youth cross-sectional study includes 1,134 YYA with type 1 diabetes (T1D, mean age: 21 years ± 5) and 274 YYA with type 2 diabetes (T2D, 25 years ± 4.2). Participants (or parents if <18 years) completed the USDA Household FS Survey Module, wherein ≥3 affirmations indicate food insecurity. Diet was assessed via food frequency questionnaire and compared to age- and sex-specific 2020-25 Dietary Guidelines for Americans (DGA) nutritional goals for daily energy, added sugar, saturated fat, calcium, fiber, iron, magnesium, potassium, sodium, vitamins C, D, and E. Associations of FS with daily intake and meeting guidelines were assessed with sex-stratified multivariate linear regression. No intake differences by FS were observed, except females with T1D consumed more sodium (+155 mg, p =.01) and percent of kilocalories from saturated fat (+0.7%, p =.04) when FS was absent. Males with T2D consumed more vitamin E (+1 mg, p =.03) when FS was present. Crude guideline adherence was poor across the board (i.e. below 60%). After multivariate adjustment for demographic, socioeconomic, clinical and diabetes characteristics, females with T1D and T2D, regardless of FS, on average met vitamin C and males met iron guidelines. Males with T2D met percent of kilocalories from saturated fat guidelines in the absence of FS. DGA adherence among YYA with diabetes is of grave concern given that diet is crucial to diabetes management. FS reflects very little enhancement in diet quality. YYA with diabetes consume excessive sodium, added sugars, and inadequate micronutrients except for vitamin C and iron, increasing risk for deficiency, weight gain, heart attack and stroke. Disclosure: H. D. Bercaw: None. C. Mercado: None. A. D. Liese: None. L. A. Reid: None. J. A. Mendoza: None. E. A. Frongillo: None. K. A. Sauder: None. B. A. Reboussin: None. E. J. Mayer-davis: None. D. Dabelea: None. S. M. Marcovina: None. [ABSTRACT FROM AUTHOR]

Screening for household food insecurity (HFI) is recommended because of its impact on diabetes management. While HFI has been shown to be associated with poor glycemic control in adults with type 2 diabetes (T2D) and youth and young adults (YYA) with type 1, the association between HFI and glycemic control in YYA with T2D is not known. This cross-sectional study included T2D YYA (n=395) from the 2015-2020 SEARCH for Diabetes in Youth Study (14-35 yrs; median age: 25.2 yrs). HFI was defined as affirming ≥3 items on the US Household Food Security Survey Module. HbA1c values were available for 271 participants. It was measured in a sample of whole blood and analyzed as a continuous variable. Glycemic control was dichotomized into optimal/suboptimal (≤9 %) vs. high risk (> 9.0%) based on HbA1c. Survey data on experiencing severe hypoglycemia or diabetic ketoacidosis (DKA) in the last 12 months was available for 292 participants. Logistic and linear regression were used for modeling the association of HFI with binary and continuous outcomes, respectively. Approximately 34% of YYA with T2D reported HFI in the past 12 months. The mean HbA1c of the YYA with HFI was 9.2%, 54% had HbA1c >9.0%, and 19% reported experiencing DKA or hypoglycemia. YYA who were food secure had a mean HbA1c of 9.5%, 54% had HbA1c >9.0%, and 8% reported acute complications. No associations were observed between HFI and HbA1c or glycemic control. YYA with HFI had 2.3 (CI: 1.1-5.2) times the odds of experiencing DKA or hypoglycemia as those without HFI, after adjusting for age, gender, race, parent education, household income, insurance, clinic, diabetes duration, use of continuous glucose monitoring, and medication regimen. Our data suggests that HFI among YYA with T2D is a quite common, affecting more than a third of our sample and is associated with acute diabetes-related complications. These results warrant longitudinal studies in this particularly vulnerable group to establish if alleviating HFI reduces frequency of DKA and hypoglycemia episodes. Disclosure: L. A. Reid: None. S. M. Marcovina: None. E. Lustigova: None. A. D. Liese: None. J. A. Mendoza: None. A. Merchant: None. M. Geraci: None. B. A. Reboussin: None. A. M. Ellyson: None. F. Malik: None. D. Dabelea: None. L. Merjaneh: None. Funding: National Institutes of Health (R01DK117461, 1UC4DK108173) [ABSTRACT FROM AUTHOR]

Hypoglycemia is a common acute complication in diabetes that is often treated with carbohydrate-rich snacks or drinks. For individuals with diabetes and household food insecurity (HFI), uncertain availability of food may increase fear of hypoglycemia (FOH). FOH may prevent individuals from optimal diabetes management. We analyzed data from 1,603 adult (≥18 years, mean 23.7 years, 80% type 1 diabetes [T1D], 20% type 2 diabetes [T2D]) and 592 youth (10.0-17.9 years, mean 15.2 years, 100% T1D) participants in the SEARCH for Diabetes in Youth Study. Adult participants and parents of youth participants completed the U.S. Household Food Security Survey, where ≥3 food insecure conditions or behaviors indicate HFI. Adults, youth, and parents of youth completed the Hypoglycemia Fear Survey that provides a behavior subscale (scores 0-60), worry subscale (scores 0-72), and total score (sum of subscales). We examined the association of HFI with FOH with general linear models, adjusting for socioeconomic, demographic, and clinical covariates. Approximately 20% of adults and 17% of youth had HFI. Adults with T1D reporting HFI (vs. no HFI) had increased FOH (+3 units for behavior, +11 units for worry, +14 units for total), after adjustment for covariates (all p<0.0001). Parents of youth with T1D reporting HFI had a 3 unit increase in worry FOH (p=0.03). Adults with T2D reporting HFI had increased FOH (+8.9 units for total, +6 units for worry; all p<0.01). Youth with T1D reporting HFI did not differ in FOH than those not reporting HFI. This research suggests that among adults with diabetes and parents of youth with T1D, those with HFI are more likely to experience FOH than those without HFI. Implementation of common approaches to ameliorate risk of hypoglycemia such as carrying snacks is problematic for people who are food insecure. Providers could consider HFI when discussing hypoglycemia and fear thereof with their patients. Disclosure: L.A. Reid: None. S. Zheng: None. J.A. Mendoza: None. B.A. Reboussin: None. K.A. Sauder: None. S. Saydah: None. J.M. Lawrence: None. E.T. Jensen: None. L. Henkin: None. K. Flory: None. L.M. Knight: None. C. Pihoker: None. L.M. Dolan: None. A.D. Liese: None. Funding: National Institutes of Health (1UC4DK108173, 1R01DK117461-01) [ABSTRACT FROM AUTHOR]

Affordability and geographic accessibility are key health care access characteristics. We used data from 481 youth and young adults (YYA) with diabetes (389 type 1, 92 type 2) to understand the association between health care access and glycemic control as measured by HbA1c values. In multivariate models, YYA with state or federal health insurance had HbA1c percentage values 0.68 higher (P = 0.0025) than the privately insured, and those without insurance 1.34 higher (P < 0.0001). Not having a routine diabetes care provider was associated with a 0.51 higher HbA1c (P = 0.048) compared to having specialist care, but HbA1c did not differ significantly (P = 0.069) between primary vs specialty care. Distance to utilized provider was not associated with HbA1c among YYA with a provider (P = 0.11). These findings underscore the central role of health insurance and indicate a need to better understand the root causes of poorer glycemic control in YYA with state/federal insurance. [ABSTRACT FROM AUTHOR]

This study examined the effects of the ActiGraph’s (AG) low-frequency extension (LFE) filter on steps and physical activity classification in the free-living environment. Thirty-four African-American women (age, 24.5±5.2 years; BMI, 24.9±4.5 kg/m2) had daily activity measured simultaneously with an AG-GT3X+ accelerometer and a New Lifestyles NL-800 pedometer for seven days. Steps per day (steps/day) and time (minutes/day) spent in sedentary, light, and moderate-to-vigorous physical activity (MVPA) were examined with and without the LFE filter (AG-LFE and AG-N, respectively). The AG-LFE recorded more total steps (13,723±4,983 steps/day) compared to AG-N and NL-800 (6,172±2,838 and 5,817±3,037 steps/day, respectively; p<0.001). Compared to the AG-N, the AG-LFE estimated less time in sedentary behaviors (518.7±92.1 vs. 504.2±105.4 min/day, respectively; p<0.001), and more time in light (247.7±70.4 vs. 279.1±74.7 min/day, respectively; p<0.001) and MVPA (18.9±16.9 vs. 21.5±18.2 min/day, respectively; p<0.001), respectively. These data suggest that steps and physical activity classifications will be affected when using the ActiGraph with and without the LFE filter. Future research should investigate the accuracy of these measures using the LFE filter. [ABSTRACT FROM AUTHOR]

Background: Organic nitrates uncouple bone turnover, improve bone mineral density, and improve trabecular and cortical components of bone. These changes in turnover, strength and geometry may translate into an important reduction in fractures. However, before proceeding with a large fracture trial, there is a need to identify the nitrate formulation that has both the greatest efficacy (with regards to bone turnover markers) and gives the fewest headaches. Ascertaining which nitrate formulation this may be is the purpose of the current study.Methods and Design: This will be an open-label randomized, controlled trial conducted at Women's College Hospital comparing five formulations of nitrates for their effects on bone turnover markers and headache. We will recruit postmenopausal women age 50 years or older with no contraindications to nitroglycerin. Our trial will consist of a run-in phase and a treatment phase. We will enroll 420 women in the run-in phase, each to receive all of the 5 potential treatments in random order for 2 days, each with a 2-day washout period between treatments. Those who tolerate all formulations will enter the 12-week treatment phase and be randomly assigned to one of five groups: 0.3 mg sublingual nitroglycerin tablet, 0.6 mg of the sublingual tablet, a 20 mg tablet of isosorbide mononitrate, a 160 mg nitroglycerin transdermal patch (used for 8 h), and 15 mg of nitroglycerin ointment as used in a previous trial by our group. We will continue enrolment until we have randomized 210 women or 35 women per group. Concentrations of bone formation (bone-specific alkaline phosphatase and procollagen type I N-terminal propeptide) and bone resorption (C-telopeptides of collagen crosslinks and N-terminal crosslinks of collagen) agents will be measured in samples taken at study entry (the start of the run in phase) and 12 weeks. Subjects will record the frequency and severity of headaches daily during the run-in phase and then monthly after that. We will use the 'multiple comparisons with the best' approach for data analyses, as this strategy allows practical considerations of ease of use and tolerability to guide selection of the preparation for future studies.Discussion: Data from this protocol will be used to develop a randomized, controlled trial of nitrates to prevent osteoporotic fractures.Trial Registration: ClinicalTrials.gov Identifier: NCT01387672. Controlled-Trials.com: ISRCTN08860742. [ABSTRACT FROM AUTHOR]

To examine, among youth and young adults (YYA) with type 1 diabetes (T1D), the association of household food insecurity (HFI) with: 1) HbA 1c and 2) episodes of diabetic ketoacidosis (DKA) and severe hypoglycemia. HFI was assessed using the U.S. Household Food Security Survey Module in SEARCH for Diabetes in Youth participants with T1D between 2016 and 2019. Linear and logistic regression models adjusted for age, diabetes duration, sex, race, ethnicity, clinic site, parent/participant education, household income, health insurance, and diabetes technology use. Of 1830 participants (mean age 20.8 ± 5.0 years, 70.0 % non-Hispanic White), HbA 1c was collected for 1060 individuals (mean HbA1c 9.2 % ± 2.0 %). The prevalence of HFI was 16.4 %. In the past 12 months, 18.2 % and 9.9 % reported an episode of DKA or severe hypoglycemia, respectively. Compared to participants who were food secure, HFI was associated with a 0.33 % (95 % CI 0.003, 0.657) higher HbA 1c level. Those with HFI had 1.58 (95 % CI 1.13, 2.21) times the adjusted odds of an episode of DKA and 1.53 (95 % CI 0.99, 2.37) times the adjusted odds of an episode of severe hypoglycemia as those without HFI. HFI is associated with higher HbA 1c levels and increased odds of DKA in YYA with T1D. [ABSTRACT FROM AUTHOR]

Background and Objectives: Otalgia can be primary/otogenic or secondary as a referred pain from another site, which can be difficult to establish owing to various causes and the complex innervation of the ear. In our center, we observed a large group of patients with unexplained otalgia that had a higher prevalence of migraine. We hypothesized that migraine may cause secondary otalgia. This study then aimed to determine the prevalence of migraine-associated otalgia and evaluate the efficacy of migraine treatment. Subjects and Methods: This 2-year retrospective study was conducted at a busy otology clinic. Patients were identified using diagnostic codes corresponding to otalgia. The prevalence of migraine-associated otalgia was determined, and the efficacy of migraine treatment was evaluated in these patients. The interventions included prophylactic and abortive migraine treatments. Statistical analysis was conducted to compare between the pre- and post-treatment symptoms. Results: A total of 208 patients with otalgia were identified. Sixty-four out of ninety patients with unexplained otalgia met the criteria for migraine; of them, 30 patients had an adequate follow-up and were thus included in the evaluation of treatment efficacy. Otalgia improved in 87% of the patients who received migraine treatment. After treatment, the mean pain score and headache frequency significantly decreased from 7 to 2 and from 27 to 9 days per month, respectively (p<0.001). Conclusions: Migraine should be considered as a source of secondary otalgia, and patients should receive treatment as they often respond to migraine treatment. [ABSTRACT FROM AUTHOR]

The studies reported here examine how participants distribute resources to arbitrary stimuli in an equivalence class after one stimulus is given a social label. In Experiment 1, two 3-member equivalence classes were established with nonsense syllables (Class 1: A1 (ZID), B1 (YIM), C1 (FAP) and Class 2: A2 (VEK), B2 (RIX), C2 (KUD)) using matching-to-sample training. A social function was then assigned to B1 only, using the simple verbal statement "YIM is a Good person." Next, participants were instructed to allocate tokens to stimuli in whatever way they consider appropriate. In general, the percentage distribution of tokens allocated to Class 1 was greater than those allocated to Class 2. Participants were then informed that a mistake had been made "Sorry I have made a mistake. YIM was actually a bad person not a good person." Participants were again asked to allocate tokens. In general, results showed a reduction in the distribution of tokens allocated to Class 1, with a relatively higher decrease for B1, and an increase in the distribution allocated to Class 2. Experiment 2 replicated Experiment 1 with the addition of a baseline assessment of token distribution prior to examining the effects of adding a social function to B1. During this baseline, the distribution of tokens was relatively similar across both classes. When social functions were added, marked differences occurred in the distribution depending on whether YIM was described as a "Good" or a "Bad" person. Results are discussed regarding transfer of function and its relevance to experimental social psychology. [ABSTRACT FROM AUTHOR]

Rationale: The stable carbon isotopic (δ13C) reference material (RM) LSVEC Li2CO3 has been found to be unsuitable for δ13C standardization work because its δ13C value increases with exposure to atmospheric CO2. A new CaCO3 RM, USGS44, has been prepared to alleviate this situation. Methods: USGS44 was prepared from 8 kg of Merck high‐purity CaCO3. Two sets of δ13C values of USGS44 were determined. The first set of values was determined by online combustion, continuous‐flow (CF) isotope‐ratio mass spectrometry (IRMS) of NBS 19 CaCO3 (δ13CVPDB = +1.95 milliurey (mUr) exactly, where mUr = 0.001 = 1‰), and LSVEC Li2CO3 (δ13CVPDB = −46.6 mUr exactly), and normalized to the two‐anchor δ13CVPDB‐LSVEC isotope‐delta scale. The second set of values was obtained by dual‐inlet (DI)‐IRMS of CO2 evolved by reaction of H3PO4 with carbonates, corrected for cross contamination, and normalized to the single‐anchor δ13CVPDB scale. Results: USGS44 is stable and isotopically homogeneous to within 0.02 mUr in 100‐μg amounts. It has a δ13CVPDB‐LSVEC value of −42.21 ± 0.05 mUr. Single‐anchor δ13CVPDB values of −42.08 ± 0.01 and −41.99 ± 0.02 mUr were determined by DI‐IRMS with corrections for cross contamination. Conclusions: The new high‐purity, well‐homogenized calcium carbonate isotopic reference material USGS44 is stable and has a δ13CVPDB‐LSVEC value of −42.21 ± 0.05 mUr for both EA/IRMS and DI‐IRMS measurements. As a carbonate relatively depleted in 13C, it is intended for daily use as a secondary isotopic reference material to normalize stable carbon isotope delta measurements to the δ13CVPDB‐LSVEC scale. It is useful in quantifying drift with time, determining mass‐dependent isotopic fractionation (linearity correction), and adjusting isotope‐ratio‐scale contraction. Due to its fine grain size (smaller than 63 μm), it is not suitable as a δ18O reference material. A δ13CVPDB‐LSVEC value of −29.99 ± 0.05 mUr was determined for NBS 22 oil. [ABSTRACT FROM AUTHOR]

Aims Chronic adventitial and medial infiltration of immune cells play an important role in the pathogenesis of abdominal aortic aneurysms (AAAs). Nicotinic acid (niacin) was shown to inhibit atherosclerosis by activating the anti-inflammatory G protein-coupled receptor GPR109A [also known as hydroxycarboxylic acid receptor 2 (HCA2)] expressed on immune cells, blunting immune activation and adventitial inflammatory cell infiltration. Here, we investigated the role of niacin and GPR109A in regulating AAA formation. Methods and results Mice were supplemented with niacin or nicotinamide, and AAA was induced by angiotensin II (AngII) infusion or calcium chloride (CaCl2) application. Niacin markedly reduced AAA formation in both AngII and CaCl2 models, diminishing adventitial immune cell infiltration, concomitant inflammatory responses, and matrix degradation. Unexpectedly, GPR109A gene deletion did not abrogate the protective effects of niacin against AAA formation, suggesting GPR109A-independent mechanisms. Interestingly, nicotinamide, which does not activate GPR109A, also inhibited AAA formation and phenocopied the effects of niacin. Mechanistically, both niacin and nicotinamide supplementation increased nicotinamide adenine dinucleotide (NAD+) levels and NAD+-dependent Sirt1 activity, which were reduced in AAA tissues. Furthermore, pharmacological inhibition of Sirt1 abrogated the protective effect of nicotinamide against AAA formation. Conclusion Niacin protects against AAA formation independent of GPR109A, most likely by serving as an NAD+ precursor. Supplementation of NAD+ using nicotinamide-related biomolecules may represent an effective and well-tolerated approach to preventing or treating AAA. [ABSTRACT FROM AUTHOR]

We report data from language testing of QN a patient who suffered an impairment of reading and spelling after a left sided stroke Most of his errors in reading words aloud were either visual malady malay or morphological younger young Doubts have been raised about the status of errors classified as morphological Perhaps they are really simply visual errors Although we were only able to conduct limited investigations with this subject the data suggest that his apparent morphological paralexias are genuinely morphological that they arise within the orthographic recognition system and so are genuine paralexias and that this morphological disturbance affects reading comprehension as well as reading aloud In contrast QN s ability to understand and produce morphological aspects of spoken language was intact. [ABSTRACT FROM AUTHOR]

Objectives: To examine, among youth and young adults (YYAs) with type 1 diabetes (T1D), the independent association of household food insecurity (HFI) with: 1) glycemic control and 2) episodes of diabetic ketoacidosis (DKA) and severe hypoglycemia. Methods: This cross-sectional study included SEARCH for Diabetes in Youth participants with T1D examined between 2016-2019. HFI was assessed using the U.S. Household Food Security Survey Module. HbA1c was measured from blood drawn at a research visit; episodes of DKA and severe hypoglycemia were collected by survey. Linear and logistic regression models adjusted for age, sex, race, income, health insurance, diabetes duration, and SEARCH site. Results: Of 1834 participants (mean age 20.8±5.0 years, 69.9% non-Hispanic white, diabetes duration 11.1±3.2 years), HbA1c was collected for 1029 individuals (mean HbA1c 9.2%±2.0%). The prevalence of HFI was 16.4%. In this sample, 18.7% and 9.9% reported an episode of DKA or severe hypoglycemia in the past 12 months, respectively. Compared to participants who were food secure, HFI was associated with a 0.43% [CI: 0.02%-0.68%] higher HbA1c. In two separate adjusted models, those with HFI had 1.86 [CI: 1.03, 2.34] times the odds of an episode of DKA and 1.64 [CI: 1.03, 2.34] times the odds of an episode of severe hypoglycemia as those without HFI. Conclusions: HFI is independently associated with poor glycemic control and increased odds of diabetes-related complications in YYAs with T1D. Interventions to reduce HFI and studies to longitudinally examine its impact on glycemic control and complications are warranted. Disclosure: F. Malik: None. A.D. Liese: None. A.M. Ellyson: None. L.A. Reid: None. K.A. Sauder: None. E.A. Frongillo: None. C. Pihoker: None. D. Dabelea: None. J.M. Lawrence: None. E.T. Jensen: None. S. Saydah: None. S.M. Marcovina: None. D. Bowlby: None. J.A. Mendoza: None. Funding: Centers for Disease Control and Prevention (00097, DP-05-069, DP-10-001); National Institute of Diabetes and Digestive and Kidney Diseases (1UC4DK108173) [ABSTRACT FROM AUTHOR]

Introduction: Neutrophils play an important role in the pathogenesis of abdominal aortic aneurysms (AAA), and genetic deletion of neutrophil myeloperoxidase (MPO) inhibits AAA formation. Niacin (nicotinic acid), a naturally occurring vitamin B3 and substrate for nicotinamide adenine dinucleotide (NAD+), has been shown to inhibit neutrophil activation and MPO release by activating the anti-inflammatory G-protein-coupled receptor GPR109A expressed on immune cells. Here, we tested the role of niacin and GPR109A in regulating AAA formation in the angiotensin II (AngII) infusion model and the calcium chloride (CaCl2) application model. Methods and Results: Treatment with niacin (0.3% in drinking water) during infusion of Ang II (1000 ng·kg-1·min-1) for 14 days in LDL receptor knockout mice, or after aortic application of CaCl2 in wild-type mice, markedly reduced the incidence and size of AAA. Niacin significantly diminished adventitial inflammatory cell infiltration, elastin degradation, levels of MMP-2/9, and aortic and plasma MPO. Unexpectedly, genetic deletion of GPR109A did not abrogate the protective effects of niacin against AAA formation in the CaCl2 model, suggesting that niacin prevents AAA via GPR109A-independent mechanisms. Interestingly, nicotinamide (0.1% or 0.4%), which does not activate GPR109A, also significantly inhibited AAA formation and phenocopied the effects of niacin. Mechanistically, we found that aortic levels of NAD+ were significantly reduced in AAA tissues and restored by nicotinamide supplementation. Furthermore, activity of NAD+-dependent enzyme PARP1, which plays a fundamental role in DNA repair, was impaired in aneurysmal aortas and restored by treatment with nicotinamide. Conclusion: Niacin protects against AAA independent of GPR109A, most likely by serving as an NAD+ precursor. Supplementation with nicotinamide or related biomolecules may represent an effective and well-tolerated approach to preventing or treating AAA. [ABSTRACT FROM AUTHOR]