7 results on '"Reis, Carolina Fernandes"'
Search Results
2. A putative OTU domain-containing protein 1 deubiquitinating enzyme is differentially expressed in thyroid cancer and identifies less-aggressive tumours
- Author
-
Reis, Carolina Fernandes, 1982, Morari, Elaine Cristina, Ward, Laura Sterian, 1956, and UNIVERSIDADE ESTADUAL DE CAMPINAS
- Subjects
Neoplasias da glândula tireóide ,Thyroid neoplasms ,Artigo original ,Phage display ,Biomarker ,ScFv antibody - Abstract
Agradecimentos: The present work was supported by the Brazilian funding agencies: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenacao de Aperfeicoamento de Pessoal de N?vel Superior (CAPES) and Conselho Nacional de Desenvolvimento Cient?fico e Tecnologico (CNPq). We thank Dr Jose Vassallo (A.C. Camargo) for performing IHC analysis, and the A. C. Camargo Hospital for providing tissue microarrays Abstract: This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis. We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of selective interactive ligands) and phage display technology. One highly reactive clone, scFv-C1, with specific binding to papillary thyroid tumour proteins was confirmed by ELISA, which was further tested against a tissue microarray that comprised of 229 thyroid tissues, including: 110 carcinomas (38 papillary thyroid carcinomas (PTCs), 42 follicular carcinomas, 30 follicular variants of PTC), 18 normal thyroid tissues, 49 nodular goitres (NG) and 52 follicular adenomas. The scFv-C1 was able to distinguish carcinomas from benign lesions (P=0.0001) and reacted preferentially against T1 and T2 tumour stages (P=0.0108). We have further identified an OTU domain-containing protein 1, DUBA-7 deubiquitinating enzyme as the scFv-binding antigen using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. The strategy of screening and identifying a cell-surface-binding antibody against thyroid tissues was highly effective and resulted in a useful biomarker that recognises malignancy among thyroid nodules and may help identify lower-risk cases that can benefit from less-aggressive management CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIG FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP Fechado
- Published
- 2014
3. Chromosomal disruption and rearrangements during murine sarcoma development converge to stable karyotypic formation kept by telomerase overexpression
- Author
-
de Oliveira-Júnior, Robson José, primary, Ueira-Vieira, Carlos, additional, Sena, Angela Aparecida Servino, additional, Reis, Carolina Fernandes, additional, Mineo, José Roberto, additional, Goulart, Luiz Ricardo, additional, and Morelli, Sandra, additional
- Published
- 2016
- Full Text
- View/download PDF
4. 15-Deoxy-Δ12,14-prostaglandin J2Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells
- Author
-
Trindade-da-Silva, Carlos Antônio, primary, Reis, Carolina Fernandes, additional, Vecchi, Lara, additional, Napimoga, Marcelo Henrique, additional, Sperandio, Marcelo, additional, Matias Colombo, Bruna França, additional, Alves, Patrícia Terra, additional, Ward, Laura Sterian, additional, Ueira-Vieira, Carlos, additional, and Goulart, Luiz Ricardo, additional
- Published
- 2016
- Full Text
- View/download PDF
5. 15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells.
- Author
-
Trindade-da-Silva, Carlos Antônio, Reis, Carolina Fernandes, Vecchi, Lara, Napimoga, Marcelo Henrique, Sperandio, Marcelo, Matias Colombo, Bruna França, Alves, Patrícia Terra, Ward, Laura Sterian, Ueira-Vieira, Carlos, and Goulart, Luiz Ricardo
- Subjects
- *
THYROID cancer treatment , *APOPTOSIS , *CANCER cells , *CYCLOPENTENONE , *INTERLEUKIN-6 , *PEROXISOME proliferator-activated receptors , *PROSTAGLANDINS - Abstract
The cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-γ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ2 shows the potential to become a new therapeutic approach for thyroid tumors. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
6. Nova plataforma diagnóstica para auxiliar na detecção do câncer de tireóide
- Author
-
Sindeval José da Silva, Jorge, Paulo Tannús, Reis, Carolina Fernandes, Goulart Filho, Luiz Ricardo, Freitas, Luiz Carlos Conti de, Tiveron, Rogério Costa, Cunha, Thúlio Marquez, and Loyola, Adriano Mota
- Subjects
Câncer de Tireoide ,Biomarcador CaT12 ,CIENCIAS DA SAUDE::MEDICINA [CNPQ] ,Marcadores biológicos de tumor ,Tireóide - Câncer ,Papillary tumor ,Tumor papilar ,Flow citometry ,Biomarker ,Ciências médicas ,Citometria de fluxo ,Thyroid cancer - Abstract
A punção aspirativa com agulha fina guiada por ultrassom (PAAF-US) é o principal método propedêutico através do exame citológico de nódulos tireoidianos. Contudo, 10 a 30% dos casos apresentam como resultados amostras inadequadas ou indeterminadas, requerendo nova PAAF-US ou biópsia com agulha grossa dirigida por US (BAG-US). No presente trabalho avaliamos a performance do marcador CaT12, previamente identificado como marcador de tumores tireoidianos malignos, para auxiliar na detecção de carcinomas em fragmentos de nódulos tireoidianos através da Citometria de Fluxo. O resultado obtido foi comparado com o anátomo-patológico, e testes diagnósticos foram feitos para avaliar a capacidade do marcador em identificar os tecidos contendo malignidade. Análises prévias de citometria de fluxo do marcador com linhagens tumorais foram otimizadas e indicaram que a marcação externa sem permeabilização celular era adequada para a detecção do tumor. Posteriormente, avaliou-se o processamento das biópsias, que foram submetidas à digestão com colagenase para posterior marcação e detecção. Foram coletadas 29 amostras de portadores de nódulos tireoidianos submetidos a PAAF-US com estratificação Bethesda. Após detecção de células tumorais, estabeleceu-se os cut-offs utilizados para os valores de positividade do CaT12, e os parâmetros diagnósticos foram sensibilidade de 86,67% e especificidade de 78,57%, com acurácia 82,76%, acima da apresentada nos exames anátomo-patológicos pós-cirúrgicos 75,8% e das PAAFs com 53,5%. Nossos resultados sugerem que a nova tecnologia poderá ser usada na análise de material aspirado tanto por PAAF-US quanto por BAG-US, sem depender dos parâmetros citológicos ou histológicos e podem significar uma mudança de paradigmas para o diagnóstico do câncer de tireoide. Ultrasound-guided Fine Needle Aspiration (USG-FNA) followed by cytological examination is a common procedure performed for diagnosis of thyroid nodules. However, 10 to 30% of the cases present indeterminate results, requiring the use of US-guided core needle biopsies. In the present work we evaluated the performance of the CaT12 biomarker, previously identified as a marker of malignant thyroid tumors, to aid in the detection of carcinomas in fragments of thyroid nodules through Flow Cytometry. Results were compared with histopathological analyses, and diagnostic tests were performed to evaluate the marker's ability to distinguish malignant tissues. Previous flow cytometric analyzes of the marker with tumor cell lines were optimized and indicated that cell labeling without permeabilization was adequate for tumor detection. Subsequently, the biopsies were processed and submitted to digestion with collagenase prior to labeling and detection. Twenty-nine samples from patients with thyroid nodules submitted to USG-FNB were collected. After detection of tumor cells, cut-off value was calculated and positivity was established. Diagnostic parameters present sensitivity of 86.67%, specificity of 78.57%, and accuracy of 82.76%, much higher than the accuracy presented by the histopathology (75.8%), and USG-FNB (53.5%) analysis. Our results suggest that the novel technology can be used in the analyses of biopsies without depending on cytological or histological parameters, and may lead us to a paradigm shift for the diagnosis of thyroid cancer. Tese (Doutorado)
- Published
- 2017
7. 15-Deoxy-Δ(12,14)-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells.
- Author
-
Trindade-da-Silva CA, Reis CF, Vecchi L, Napimoga MH, Sperandio M, Matias Colombo BF, Alves PT, Ward LS, Ueira-Vieira C, and Goulart LR
- Abstract
The cyclopentenone prostaglandin 15-deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-γ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ2 shows the potential to become a new therapeutic approach for thyroid tumors.
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.