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Dried distiller grains with solubles (DDGS) are a popular byproduct used in the livestock feed industry as it is a cost-effective feedstuff that is rich in crude protein and minerals. However, a common concern related to the use of DDGS in livestock diets is the negative impact that affects animal dry matter intake (DMI) and growth. To further investigate this challenge, the experiments aimed to evaluate the effect of feeding 1) two different diets, control versus heat processed DDGS or 2) increasing the concentration of DDGS and its effect on body weight (BW), average daily gain (ADG), DMI, and gain to feed ratio (G:F) in finishing lambs. In experiment 1, both diets were the same except for the heat processing of DDGS; the control diet used a commercial DDGS source directly from the supplier and the processed diet contained the same commercial DDGS source that differed through additional heat processing. For DDGS heat processing, 40% moisture was added to the DDGS and heated at 150 °C for 70 min. After the heating process, the DDGS was placed in a second oven at 56 °C for 2 d to allow for humidity evaporation. Lambs ( n  = 34), blocked by sex and BW, were housed in pens (2 to 3 lambs/pen, 6 pens/treatment) and were fed for 42 d. Lamb DMI was measured daily, and BW was measured every 14 d. In experiment 2, lambs (n = 75) were blocked by BW (5 lambs/ pen, 5 pens/treatment) and fed diets differing in inclusion rates of DDGS (10%, 20%, and 30%). The feeding period lasted for 56 d, and lamb DMI and BW were measured daily or every 28 d, respectively. In both experiments, diets were isonitrogenous, and the feeding procedure utilized slick bunk management. Data were analyzed in SAS using a mixed model considering the treatments as fixed effects and pen and block as random effects. In experiment 1, there were no treatment differences ( P  ≥ 0.13) in ADG, DMI, and G:F in finishing lambs. In experiment 2, a linear and quadratic contrast were used to separate the mean and demonstrated that increasing the concentration of DDGS decreased lamb DMI (linear P  < 0.01; quadratic P  = 0.05), but did not affect lamb BW, ADG, or G:F ( P  ≥ 0.26). Despite that previous research shows differences in animal growth because of an increase in DDGS concentration, our experiments illustrated that heat processing or increased concentrations of DDGS up to 30% in finishing lamb diets are not responsible for a decrease in lamb performance., Competing Interests: None., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objective of the current experiment was to evaluate the effects of supplementation with different dietary fatty acid profiles on the dam during the first third of gestation and on the offspring during growth on the offspring's cognitive behavior. Seventy-nine postweaning lambs were blocked by body weight and sex using a 2 × 2 factorial arrangement of treatments. The first factor (maternal supplementation; MS ) was supplementation to the ewes in the first third of gestation with 1.61% Ca salts of palm fatty acid distillate ( PFAD ) or Ca salts enriched with eicosapentaenoic ( EPA ) and docosahexaenoic acids ( DHA ) to early pregnant ewes. The second factor (offspring supplementation; OS ) was to supplement the offspring during their growing phase with diets that contained 1.48% of PFAD or Ca salts of PFAD or EPA and DHA. Ewes were housed in groups, 3 ewes per pen and 12 pens per treatment, with different treatments until day 50 of gestation. From day 51 of gestation until weaning, all the animals (ewes and lambs) were housed in a common pen. After weaning, lambs were housed in group pens (5 pens per treatment, 3 to 5 per pen). The lambs ran maze tests on weeks 5 and 7 after weaning to evaluate cognitive ability. The maze contained 2 trap zones and had the pen conspecific lambs at the end of the maze. The measurements were the times to solve the traps and the total time to complete the maze. Data were analyzed using a mixed procedure considering the 2×2 factorial arrangement of treatments. There was an MS × OS × time interaction for the time to complete the maze ( P = 0.02). Lambs receiving a different type of fatty acid supplementation during gestation than postweaning took less time to complete the maze on the second relative to the first day compared with the lambs fed the same type of fatty acids during gestation and growing. In conclusion, combining different fatty acids during different life stages may improve lambs' cognitive abilities., Competing Interests: The authors declare no conflict of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Subclinical mastitis reduces milk yield and elicits undesirable changes in milk composition, but the mechanisms resulting in reduced milk production in affected mammary glands are incompletely understood. This study investigated the effects of sterile inflammation on mammary gland metabolism by assessing changes in milk and venous blood composition. Mid-lactation primiparous Holstein cows (n = 4) had udder halves randomly allocated to treatments; quarters of 1 udder half were infused with 2 billion cfu of formalin-fixed Staphylococcus aureus (FX-STAPH) and quarters of the opposite udder half were infused with saline (SAL). Blood samples were collected from the right and left subcutaneous abdominal veins in 2.6 h intervals until 40 h postchallenge and analyzed for blood gas and metabolite concentrations. Milk from FX-STAPH udder halves had significantly increased SCS by the first milking at 8 h postchallenge. By 16 h postchallenge, FX-STAPH udder halves had increased concentrations of protein and lactate and lower lactose concentrations than SAL udder halves. Milk fat concentrations, milk yields, ECM yields, and the ferric reducing antioxidant power of milk were not significantly different between SAL and FX-STAPH udder halves. Venous blood of FX-STAPH halves had marginally greater concentrations of saturated O 2 , partial pressures of O 2 , and glucose concentrations than SAL halves. Conversely, total and partial pressures of CO 2 did not differ between udder half treatments, suggesting a shift in local metabolite utilization in FX-STAPH udder halves. These results indicate that changes in milk composition resulting from mastitis are accompanied by changes in some key blood metabolite concentrations. The shift in venous blood metabolite concentrations, along with the marked increase in milk lactate, suggests that local mammary tissue or recruited immune cells, or both, alter metabolite usage in mammary tissues. Future studies are needed to quantify the uptake of key milk precursors during mastitis., (© 2024, The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)

Oxidative stress may affect new born calves due to high stress suffered around birth. We hypothesized that maternal supplementation with micronutrients and vitamins in late gestation enhance the neonatal calf's antioxidant system, decreasing the occurrence and duration of diarrhea, and improving growth from birth through weaning. To test this hypothesis, 80 multiparous cows were cluster-assigned to treatment groups. Treated group (TG) cows received mineral and vitamin supplementation while control group (CG) cows received saline solution. Feed intake and fecal score were measured daily until the ninth week. Weight and body measurements were registered weekly, and blood samples were collected from postpartum cows and calves after birth and at 7, 14, and 63 days of life. Although CG calves had greater fecal scores ( p = 0.01), diarrhea characteristics did not differ. Calves in the TG showed greater starter intake ( p = 0.04). Feed efficiency showed a trend with treatment-age interaction ( p = 0.06). Calves in the CG had wider hips in the first week ( p = 0.03), but not by the ninth week. Total antioxidant status, thiobarbituric acid reactive substances, and haptoglobin did not differ between treatment groups. Serum metabolites showed no differences. Supplementation did not impact calf antioxidant system or growth in the first two months., Competing Interests: The authors declare no conflicts of interest.

An experiment was conducted to evaluate the effects of feeding Enogen feed corn ( EFC ) silage or EFC grain with different grain processing (dry-rolled corn vs. whole-shelled corn) in feedlot cattle diets. Total 68 Angus cross-bred steers were blocked by body weight and the treatments (diets) were randomly assigned to steers in each block: a basal diet with isoline corn silage and isoline dry-rolled corn grain ( IIR ); the basal diet with EFC silage and isoline dry-rolled corn grain ( EIR ); the basal diet with EFC silage and EFC dry-rolled grain ( EER ); and the basal diet with EFC silage and EFC whole-shelled grain ( EEW ). Isoline refers to the isogenic counterpart of Enogen corn silage or grain. Steers received the assigned treatment over 32 wk of the entire experiment (backgrounding and finishing) until harvested. Part of the steers (eight blocks) in each treatment were used to measure CH 4 production (g/d) using the GreenFeed and CH 4 production per unit of DMI. All data were analyzed using a mixed procedure of SAS in a randomized complete block design, considering diet as a fixed effect and block as a random effect. Steers fed the EIR diet increased ( P  = 0.03) DMI compared to IIR during the backgrounding phase. However, feeding EFC silage or grain did not affect body weight, average daily gain, and feed efficiency during backgrounding and finishing phases. Feeding EEW decreased ( P  ≤ 0.05) body weight, average daily gain, feed efficiency, and tended to decrease ( P  = 0.06) hot carcass weight compared to EER during the finishing phase. Methane production per unit of DMI decreased ( P  = 0.02) for steers fed EIR compared with steers fed IIR only during the backgrounding phase. Feeding EFC grain had no effect on CH 4 production (g/d) in both phases. In conclusion, feeding EFC silage or grain did not improve the performance of beef steers during the backgrounding and finishing phases in the current experiment condition. Methane production per unit of DMI was reduced for steers fed EFC silage compared with isoline corn silage only during the backgrounding phase., Competing Interests: The authors declare no conflicts of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Cull cows represent a significant percentage of revenue received from the U.S. beef industry; however, cull cows are heavily price discounted at time of slaughter. This experiment's objective is to evaluate different feeding strategies and their effects on body condition score, subcutaneous fat color, and carcass yield and quality traits in cull cows. The central hypothesis is feeding a high-energy diet, with low levels of vitamin A, for 56 d will improve animal performance, carcass yield, and quality traits in addition to capturing the point (rate) of the conversion of yellow to white subcutaneous fat. In the present experiment 98 Angus crossbreed cows were utilized. Cows were fed either low vitamin A ( LVA ) diet consisting of whole shelled corn, soybean hulls, soybean meal, and a mineral-vitamin supplement or high vitamin A ( HVA ) diet, formulated using whole shelled corn, fescue hay, dry distiller grains with soluble, and a mineral-vitamin supplement for 56 d. During the 56 d feeding period, body weights and condition scores, and subcutaneous adipose samples were collected every 14 d. On day 56, cattle were slaughtered; 48 h postmortem carcass characteristics and objective color scores (subcutaneous adipose tissue) were recorded and a sample of the longissimus dorsi lumborum was collected. Subcutaneous adipose tissue samples were utilized to record subjective color scores and then ground to be analyzed for β-carotene concentration. The longissimus dorsi lumborum samples (2.54 cm slices) were removed for Warner-Bratzler shear force ( WBSF ) and pH testing. Data were analyzed using the MIXED procedure of SAS. Feeding cull cows LVA resulted in differences in subcutaneous carcass fat color ( P  = 0.01) as well as b * values ( P  < 0.01) on day 56 compared with HVA. Subjective fat color scores were not different ( P  > 0.10) on day 0 or 14 but were different ( P  ≤ 0.05) on days 28, 42, and 56. Additionally, 9-cis-β-carotene concentration on day 56 were different ( P  = 0.05) between treatments. A trend was noticed for all- trans -β-carotene concentration ( P  = 0.10) on day 56 as well. Cull cow body weights were greater ( P  ≤ 0.04) when fed the LVA diet starting on days 14, 28, and 42; and a trend was noticed on day 56 ( P  = 0.09). Overall, cows fed the LVA treatment for 56 d exhibited decreased adipose yellowness and β-carotene concentrations as well as increased live weights., Competing Interests: None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Fetal programming research conducted in sheep has reported sexually dimorphic responses on growth of the progeny born to in-utero methionine or omega-3 fatty acids supplementation. However, the biological mechanism behind the nutrient by sex interaction as a source of variation in offspring body weight is still unknown. A high-throughput RNA sequencing data of hypothalamus samples from 17 lambs were used in the current study to identify differentially expressed genes (DEGs) between males and females born to dams supplemented with different nutrients during late-gestation. Ewes received a basal diet without omega-3 fatty acids or methionine supplementation as the control (CONT); omega-3 fatty acids supplementation (FAS), or methionine supplementation (METS). A list of regulated genes was generated. Data were compared as CONT vs. FAS and CONT vs. METS. For CONT vs. METS, a treatment by sex interaction was found (adjusted P-value < 0.05) on 121 DEGs (112 upregulated and 9 downregulated) on female lambs born to METS compared with METS males. Importantly, with the sex interaction term, more than 100 genes were upregulated in female lamb's hypothalamuses born to METS. Gene Ontology (GO) and Ingenuity Pathway Analysis (IPA) were performed using the DEGs from female lambs. Terms under biological process (related to morphogenesis, organism, and tissue development), cellular component (related to chromatin, extracellular components), and molecular function (involved in chromatin structure and transcription and factors linked to binding DNA) were presented (adjusted P-value < 0.05) for GO. For the IPA, the top-scoring network was developmental disorder, endocrine system development and function, and organ morphology. Only a few differences were observed in the comparison between the interaction of sex and treatment for the CONT vs. FAS comparison. The markedly increased number of DEGs substantially involved in developmental and growth processes indicates the extent to which maternal methionine supplementation causes the sexually dimorphic effects observed in the offspring., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

This study determined the effect of dietary calcium propionate (CaPr) as a source of energy supplementation during the First Half of Gestation (FMG), the Second Half of Gestation (SMG), and during All Gestation (AG), on offspring post-weaning growth performance, meat quality, and meat metabolomic profile. Thirty-one pregnant ewes were assigned to one of four treatments: a) supplementation of 30 gd-1 of CaPr during the first half of gestation (day 1 to day 75, n = 8) (FMG); b) supplementation of 30 gd-1 of CaPr during the second half of gestation (day 76 to day 150, n = 8) (SMG); c) supplementation of 30 gd-1 of CaPr during all gestation (AG, n = 8); d) no CaPr supplementation (control; CS, n = 7). The ewes were ad libitum fed a basal diet based on oat hay and corn silage. Ewes were distributed in a completely randomized unbalanced design to four treatments. The FMG group had lower (P ≤ 0.05) birth weight and weaning weight than the CS group. However, the average daily gain was similar across all treatments. Empty body weight and FMG had lower values (P ≤ 0.05) than the other groups. Both FMG and AG had lower hot carcass weight (P ≤ 0.05) compared to CS, while CaPr treatments resulted in reduced hot carcass yield (P ≤ 0.05). Meat color and texture were similar among treatments. A principal component analysis between gestation stages showed a trend for separating CS and FMG from SMG and AG, and that was explained by 93.7% of the data variability (PC1 = 87.9% and PC2 = 5.8%). Regarding meat metabolomic profile, 23 compounds were positively correlated between all treatments. Only 2 were negatively correlated (eicosane and naphthalene 1,2,3); but tetradecanoic acid, hexadecane, undecane 5-methyl, (-)-alpha, hexadecenoic acid, octadecanoic acid, and octadecane had a highly significant correlation (P ≤ 0.05). Overall, dam supplementation with CaPr during different periods of gestation provoked changes in meat metabolites related to the biosynthesis of fatty acids in lambs without negative changes in lamb's growth performance and carcass quality., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Pérez Segura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

One hundred and twenty-one Angus × SimAngus-crossbred steers (body weight ( BW ) = 159 ± 22 kg) were used to evaluate the effects of different grain inclusion ( GI ) rates in diets provided to early-weaned calves and steroidal implants ( SI ) utilization on growth performance and carcass characteristics, particularly intramuscular fat deposition, of beef steers. The experiment was conducted as a randomized complete block design with a 2 × 2 factorial arrangement of treatments, consisting of two GI rates (35% vs. 58%, dry matter ( DM ) basis), each one associated or not to steroidal implant utilization (no implants vs. 80 mg trenbolone acetate ( TA ) + 16 mg estradiol followed by 120 mg TA + 24 mg of estradiol). After being early-weaned (124 ± 14 d of age), steers were offered an average of 4.5 kg/d (DM basis) of a concentrate-based diet with a greater or lesser GI rate for 60 d. After being fed a concentrate-based diet with different GI rates for 60 d, steers were fed a common backgrounding diet for 56 d and subsequently fed a common high-grain diet until harvested at a constant final BW (620 kg). Steers were not implanted until the beginning of the backgrounding phase and then re-implanted when initiating the finishing phase. Data were analyzed using PROC MIXED in SAS. There were no GI × SI interactions ( P  ≥ 0.62) for any of the growth performance parameters throughout the experimental period. Implanted steers tended to have a greater average daily gain ( P  = 0.10) during the finishing phase than nonimplanted steers. For the 12th rib fat thickness and yield grade ( YG ), a GI × SI interaction ( P  = 0.03) and a tendency for a GI × SI interaction ( P  = 0.10) was detected, respectively. Nonimplanted steers fed diets with greater GI rates presented the greatest 12th rib fat thickness and tended to have the greatest YG among treatments. No other interactions ( P  ≥ 0.33) were observed for the hot carcass weight, Longissimus muscle ( LM ) area, quality grade, marbling score, and kidney-pelvic-heart fat content. Steers fed diets with lesser GI rates tended to have a greater LM area than steers fed diets with greater GI rates ( P  = 0.10). Results from this experiment indicate that varying GI rates in diets provided to early-weaned calves and subsequent implantation with steroidal hormones did not affect marbling deposition., Competing Interests: The authors declare no real or perceived conflicts of interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Background: In dogs, dietary omega 3 polyunsaturated fatty acids (n-3 PUFA) affect the fatty acid (FA) profile of blood plasma, erythrocyte membrane (EM), and semen, but their correlation has not yet been investigated., Aim: In this study, we evaluated the association between dietary PUFA and their profile in blood plasma, EM, and semen of dogs, with the possibility to predict the semen profile using the values of the three first., Methods: Twelve male dogs received the same standard commercial diet for 4 weeks. The FA profile was analyzed by gas chromatography in paired diet, blood (plasma and EM determinations), and semen samples. Data were analyzed with SAS Proc Corr version 9.4. Pearson´s correlation coefficient (significant if p < 0.05) was used to assess the association of dietary FA profiles with those in blood plasma, EM, and semen., Results: There was a positive correlation between dietary eicosapentaenoic acid (EPA) and blood plasma ( r = 0.97), EM ( r = 0.94) and semen ( r = 0.92) EPA, and between dietary docosahexaenoic acid (DHA) and arachidonic acid (ARA) and semen DHA ( r = 0.93) and ARA ( r = 0.92), respectively. There was a negative correlation between dihomo-gamma-linolenic acid (DGLA) in the diet and EM DGLA ( r = -0.94)., Conclusion: The dietary EPA is correlated with blood plasma, EM, and semen EPA concentrations, and dietary DHA and ARA are associated with semen DHA and ARA concentrations in dogs. These findings suggest that dietary EPA, DHA, and ARA concentrations could be helpful to predictive markers for such concentrations in the semen of dogs., Competing Interests: The authors declare that there is no conflict of interest.

This study evaluated the feedlot performance, carcass characteristics, and fatty acid (FA) composition of Wagyu-sired and Angus-sired cattle at a similar days on feed (D) or body weight (B) endpoint. Wagyu-sired steers and heifers (WA) from two different sires, selected for either growth (G) or marbling (M), were compared with Angus-sired steers (AN, n = 13) in two independent incomplete-block design experiments at a similar days on feed (DOF; WA-GD, n = 9; WA-MD, n = 12) in experiment 1 or similar final body weight (BW; WA-GB, n = 9; WA-MB, n = 13) in experiment 2. Cattle were offered a corn silage-based growing diet for 119 d before being transitioned over 3 wk to a finishing diet. Data were analyzed as a randomized incomplete block design. In experiment 1, AN and WA-GD cattle had a greater (P < 0.01) off-test BW and average daily gain (ADGP < 0.04) than WA-MD cattle and AN had a greater dry matter intake (DMI; P < 0.02) than WA-GD and WA-MD cattle. The AN and WA-GD cattle had a greater carcass weight (P < 0.02) than WA-MD cattle. Percent kidney, pelvic, and heart fat (KPH) was greatest (P < 0.01) for WA-MD cattle, followed by WA-GD cattle, and lastly AN cattle. Total lipid (P < 0.03) and polyunsaturated FA (PUFA; P < 0.01) percentage were greater for WA-MD-cattle than AN and WA-GD cattle, as they tended (P = 0.11) to have the greatest 12th rib marbling score. In experiment 2, AN-cattle needed fewer (P < 0.01) DOF and had a greater (P ≤ 0.02) ADG, DMI, and gain:feed than WA-GB- and WA-MB-cattle. The WA-MB-cattle had a greater (P < 0.01) 12th and 6th rib marbling score, USDA quality grade, and 6th rib backfat thickness than AN-cattle. Compared with AN cattle, WA-GB cattle had a greater (P ≤ 0.01) percent KPH and lesser (P ≤ 0.03) rib thickness. The WA-MB-cattle had a greater (P ≤ 0.01) concentration of total lipid and PUFA than AN- and WA-GB-cattle, and lesser saturated FA (SFA; P < 0.01) concentration than AN-cattle in the longissimus muscle (LM). The 6th rib location of the LM had a greater (P ≤ 0.01) percentage of total lipid and SFA, but less (P < 0.03) MUFA compared with the 12th rib location. In conclusion, Wagyu-sired cattle selected for marbling potential had a lesser ADG, DMI, more marbling, more PUFA, and less SFA in the LM than Angus-sired cattle regardless of slaughter endpoint. Wagyu-sired cattle selected for growth potential had a similar ADG and carcass characteristics compared with Angus-sired cattle when fed for a similar number of days on feed., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The aim of this experiment was to evaluate the effect of increasing dietary omega-3 (n-3) polyunsaturated fatty acid (PUFA) supplementation on plasma and follicular fluid resolvin D1 (RvD1) concentration and the mRNA expression of genes related to RvD1 production, inflammatory response, oxidative stress, hormone receptors and production, and free fatty acid receptors in the granulosa cells of ewes. Dorset × Hampshire ewes (n = 24) aged 2 to 4 yr and with an initial body weight (BW) of 84.08 ± 13.18 kg were blocked by body condition score (BCS) and BW, and randomly assigned to 12 pens. Each pen within each block was randomly assigned to one of three treatments: 1) diet without fatty acid supplementation (control), 2) diet with 0.5% n-3 PUFA supplementation (PUFA0.5), and 3) diet with 1% n-3 PUFA supplementation (PUFA1). BW, BCS, and blood samples were obtained on day 1 and every 21 d for 3 mo. Ewes were then synchronized, superstimulated, and ovariectomized. Antral follicles were aspirated to evaluate RvD1 concentration in follicular fluid, and granulosa cells were used to determine mRNA abundance. Data were analyzed as a randomized complete block design using a mixed model (MIXED or GLIMMIX with log as a link function when data presented a nonnormal distribution). A polynomial effect of treatments was used to analyze RvD1 concentration and mRNA expression when there was no interaction. In addition, the correlation between plasma and follicular fluid RvD1 concentration was evaluated. We found no differences in BW (P = 0.28) and BCS (P = 0.29) between treatments. The concentration of RvD1 in plasma and follicular fluid linearly increased (P = 0.03) and tended to increase (P = 0.06) concomitantly to increasing PUFA supplementation. Plasma and follicular fluid RvD1 concentrations were positively correlated (r = 0.61; P < 0.01). The abundance of GPX1 and GPR32 mRNA tended to increase linearly with increasing PUFA supplementation (P = 0.06). In addition, PUFA supplementation linearly decreased and tended to decrease IL-1β and COX-2 mRNA abundance (P = 0.01 and P = 0.06, respectively). In conclusion, the correlation between plasma and follicular fluid RvD1 concentration indicates a relationship between both compartments. Also, the decrease of IL-1β and the increase of GPX1 mRNA abundance after PUFA supplementation could have beneficial effects on follicle development., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

We evaluate the effect of the weaning strategy (WS; early, 130 ± 21 d vs. normal, 187 ± 20 d) and backgrounding management (BGM) on growth, carcass characteristics, and relative mRNA expression in the longissimus muscle (LM) of beef steers. One hundred and twenty Angus × SimAngus-crossbred steers (body weight (BW) = 130 ± 11.2 kg) were used in a randomized complete block design. Steers, blocked by age and BW, were randomly assigned to one treatment (2 × 2 factorial). Treatments consisted of early weaned (EW) or normal weaned (NW) steers subsequently backgrounded (BG) on either a forage-based (FB) or concentrates-based (CB) diet. The EW steers (d 0) were ad libitum-fed a grain-based diet for 49 d until nursing calves were NW. Steers were ad libitum-fed either a FB diet for 214 d or a CB diet for 95 d afterward. Steers were finished on a high-grain diet until harvested at an estimated constant 12th-rib fat thickness (1.5 cm). Expression of mRNA in the LM was measured over time. Data were analyzed using PROC MIXED in SAS. The EW steers were heavier (P ˂ 0.01) at the beginning of the backgrounding and finishing period. When the finishing phase began, steers FB were heavier (P ˂ 0.01) than CB steers. There was a tendency for a WS × BGM interaction (P = 0.08) for final BW, where NW-FB steers were heavier than the steers on the other three treatments, which do not differ between them. In the finishing phase, steers BG on a FB diet had greater dry matter intake and average daily gain, but lower gain-to-feed ratio (P ˂ 0.01). There was a WS × BGM interaction (P = 0.03) for days on feed (DOF) in the finishing diet, where backgrounding steers in a FB diet decreased DOF required to reach the harvesting target among EW steers, but not within NW steers. No interactions or treatment effects (P ≥ 0.17) were detected for marbling score (MS). For ZFP423, EW steers showed a greater mRNA expression on d 112 and a lower expression on d 255 than NW steers (P ˂ 0.01). In d 57, steers BG on a CB diet presented a greater delta-like homolog 1 mRNA expression than steers BG on a FB diet, whereas in d 255, this was inverted (P ˂ 0.01). For CCAAT/enhancer binding protein D (C/EBPD) mRNA expression, a tendency for a WS × BGM interaction was observed (P = 0.06), where a greater expression of C/EBPD was observed in steers BG on a FB diet among EW steers, but not within NW steers. In this study, early grain feeding followed by different BGM does not support MS improvements of beef carcasses., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

This study investigates intramuscular (IM) adipocyte development in the Longissimus muscle (LM) between Wagyu- and Angus-sired steers compared at a similar age and days on feed (D) endpoint or similar body weight (B) endpoint by measuring IM adipocyte cell area and lipid metabolism mRNA expression. Angus-sired steers (AN, n = 6) were compared with steers from two different Wagyu sires (WA), selected for either growth (G) or marbling (M), to be compared at a similar days on feed (DOF; 258 ± 26.7 d; WA-GD, n = 5 and WA-MD, n = 5) in Exp. 1 or body weight (BW; 613 ± 18.0 kg; WA-GB, n = 4 and WA-MB, n = 5) in Exp. 2, respectively. In Exp. 1, WA-MD steers had a greater (P ≤ 0.01) percentage of IM fat in the LM compared with AN and WA-GD steers. In Exp. 2, WA-MB steers had a greater (P ≤ 0.01) percentage of IM fat in the LM compared with AN and WA-GB steers. The distribution of IM adipocyte area was unimodal at all biopsy collections, with IM adipocyte area becoming progressively larger as cattle age (P ≤ 0.01) and BW increased (P ≤ 0.01). Peroxisome proliferator activated receptor delta (PPARd) was upregulated earlier for WA-MD and WA-MB cattle compared with other steers at a similar DOF and BW (P ≤ 0.02; treatment × biopsy interaction). Peroxisome proliferator activated receptor gamma was upregulated (PPARg) at a lesser BW for WA-MB steers (P = 0.09; treatment × biopsy interaction), while WA-MD steers had a greater (P ≤ 0.04) overall mean PPARg mRNA expression compared with other steers. Glycerol-3-phosphate acyltransferase, lipin 1, and hormone sensitive lipase demonstrated mRNA expression patterns similar to PPARg and PPARd or CCAAT enhancer binding protein beta, which emphasizes their importance in marbling development and growth. Additionally, WA-MD and WA-MB steers often had a greater early mRNA expression of fatty acid transporters (fatty acid transport protein 1; P < 0.02; treatment × biopsy interaction) and binding proteins (fatty acid binding protein 4) compared with other steers. Cattle with a greater marbling propensity appear to upregulate adipogenesis at a younger chronological and physiological maturity through PPARd, PPARg, and possibly adipogenic regulating compounds, lysophosphatidic acid, and diacylglycerol. These genes and compounds could be used as potential markers for marbling propensity of cattle in the future., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Winter and spring precipitation are predicted to increase in the Midwest region of the United States, causing muddy conditions. In a previous experiment, Angus cows (8 per treatment) were paired based on initial body weight (BW) and one cow from each pair was randomly allocated to either the mud or control treatment. Though cows consumed the same amount of dry matter, cows in the mud treatment weighed 37.4 kg less than cows in the control treatment by day 269 of gestation. The objective of this experiment was to evaluate developmental programming effects of steers born to cows in the mud treatment (MUD; n = 7) or the control treatment (CON; n = 6). Steers were weighed at birth and then weekly from approximately 56 d of age until weaning and were subjected to a glucose tolerance test (GTT) and adrenocorticotropic hormone (ACTH) challenge after weaning. Steers were then placed in the feedlot for an 84-d growing phase and were weighed weekly and 12th rib back fat (BF) and ribeye area (REA) were imaged every 28 d using ultrasonography. Data were analyzed as a randomized complete block design with repeated measurements when appropriate (SAS 9.4). Although there was a 37.4 kg decrease in BW of cows by the end of gestation, there was no evidence of a pen treatment effect on calf birth weight (P = 0.60) or weaning weight (P = 0.99). Additionally, there was no evidence of a pen treatment × day effect for steer BW from birth to weaning (P = 0.67) or growing phase BW (P = 0.60). There was evidence of a treatment × day of growing phase effect (P = 0.02) for BF, such that CON steers had greater BF on day 28 of the growing phase; however, there was no evidence of a treatment × day effect for REA (P = 0.20). Furthermore, there was no evidence of a pen treatment effect for the growing phase average daily gain (P = 0.74), dry matter intake (P = 0.65), gain:feed (P = 0.48), plasma glucose concentration (P = 0.67) or plasma insulin concentration (P = 0.61) in response to the GTT, or plasma cortisol concentration in response to the ACTH challenge (P = 0.51). These results indicate that while mud increased net energy requirements for cows in the MUD treatment, there were no subsequent effects observed for steer BW, gain:feed, or response to glucose and ACTH during the growing phase., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Our objectives were to assess the effects of eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) supplementation to pregnant ewes during the first third of gestation on their offspring's liver, adipose, and muscle tissues fatty acid (FA) profile and liver mRNA expression after a finishing period receiving diets with different FA profiles. Twenty-four post-weaning lambs, blocked by sex and body weight, were used in a 2 × 2 factorial arrangement of treatments. The first factor was dam supplementation (DS) in the first third of gestation with 1.61% of Ca salts of palm fatty acid distillate (PFAD) or Ca salts enriched with EPA-DHA. Ewes were exposed to rams with marking paint harnesses during the breeding. Ewes started DS at the day of mating, considered day 1 of conception. Twenty-eight days after mating, ultrasonography was used to confirm pregnancy, and nonpregnant ewes were removed from the groups. After weaning, the offspring lambs were supplemented (LS, second main factor) with two different FA sources (1.48% of PFAD or 1.48% of EPA-DHA) during the growing and fattening phase. Lambs were fed the LS diet for 56 d and sent to slaughter, where the liver, muscle, and adipose tissue samples were collected for FA analysis. Liver samples were collected for relative mRNA expression for genes associated with FA transport and metabolism. The data were analyzed as a mixed model in SAS (9.4). In the liver, the amount of C20:5 and C22:6 (P < 0.01) increased in lambs with LS-EPA-DHA, while some C18:1 cis FA isomers were greater in the lambs from DS-PFAD. In muscle, amounts of C22:1, C20:5, and C22:5 increased (P < 0.05) in lambs born from DS-EPA-DHA. The adipose tissue amounts of C20:5, C22:5, and C22:6 were greater (P < 0.01) in lambs from LS-EPA-DHA. Interactions (DS × LS; P < 0.05) were observed for DNMT3β, FABP-1, FABP-5, SCD, and SREBP-1; having greater mRNA expression in liver tissue of LS-EPA-DHA, DS-PFAD and LS-PFAD, DS-EPA-DHA lambs compared with the lambs in the other two treatments. Liver ELOVL2 mRNA relative expression (P < 0.03) was greater in the offspring of DS-PFAD. Relative mRNA expression (P < 0.05) of GLUT1, IGF-1, LPL, and PPARγ increased in the liver from LS-EPA-DHA lambs. Dam supplementation during early gestation using with different FA sources changed the lipid FA profile in MT, LT, and SAT during the finishing period depending on the tissue and type of FA source administered during the growing phase., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

To evaluate the effect of supplementing beef cattle with a ruminal probiotic consisting of native rumen microbes (NRM; Chordicoccus furentiruminis, Prevotella albensis, and Succinivibrio dextrinosolvens) on methane (CH4) emissions, growth performance, carcass characteristics, and plasma metabolites, Angus × SimAngus-crossbred steers (n = 32; 8 per pen) and heifers (n = 48; 12 per pen) with an initial body weight (BW) of 353 ± 64 kg were used in randomized complete block design. Cattle were blocked by sex and BW and randomly assigned to 1 of 2 treatments (2 pens per treatment). Treatments consisted of diets offered for ad libitum intake with (NRM) or without (CON) the inclusion of the ruminal probiotic. Cattle were fed a growing diet for 49 d followed by a ground corn-based diet for 124 ± 27 d until reaching the targeted final BW (635 kg for steers and 590 kg for heifers). Methane emissions were estimated using the GreenFeed system (n = 12 per treatment) prior to trial commencement (baseline; period 1), and on three (2, 3, and 4), and two (5 and 6) different sampling periods throughout the growing and finishing stage, respectively. All data were analyzed using the PROC MIXED procedure of SAS. For CH4 production (g/d), there was a tendency for an NRM supplementation × period interaction (P = 0.07) where cattle-fed diets with NRM had lower production of methane in periods 3 and 4. Including NRM in the diet decreased CH4 yield (g/kg of dry matter intake (DMI)) by 20%. For CH4 emission intensity (g/kg of average daily gain (ADG)), an interaction (P < 0.01) of NRM supplementation × period occurred. In periods 2 and 3, cattle-fed diets with NRM inclusion had lower CH4 emission intensity than CON cattle. During the 84-d period when all cattle were still on the finishing diet, feeding NRM increased (P = 0.02) ADG and tended to increase (P = 0.10) DMI. At the end of the 84-d period, cattle-fed NRM tended to be heavier (P = 0.06) than CON cattle. Cattle supplemented with NRM required less (P = 0.04) days on feed to reach the targeted final BW. No differences (P ≤ 0.11) were detected for gain-to-feed ratio and carcass characteristics. Cattle-fed NRM had greater abundance of uncultured rumen bacteria that may improve rumen digestion when fed a high grain diet and potentially promote the reduction of enteric CH4 production. Results from this study suggest that daily administration of NRM may be a strategy to mitigate methanogenesis and improve the growth performance of beef cattle., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

This experiment evaluated growth, glucose metabolism, carcass characteristics, and meat quality of market lambs that were offered ad libitum or restricted (85% of ad libitum) feed intake following two different maternal fatty acid (FA) supplementations while in-utero. Ewes received either a diet supplemented with polyunsaturated FA or saturated/monounsaturated FA during mid- to late-gestation. Following weaning, progeny wethers were fed either ad libitum or a restricted level of feed intake. Ewe FA supplementation did not affect (P ≥ 0.11) growth, meat quality, nor plasma glucose or insulin concentrations of the progeny. Carcass body fat and yield grade of the progeny were affected (P = 0.01) by maternal FA supplementation and restricted feed intake. In summary, maternal FA supplementation did not affect progeny growth, while feed restriction during finishing did not affect meat quality. The interaction between maternal FA supplementation and finishing strategy for body fat accretion indicates that metabolism and the supply of FA during gestation may warrant further investigation., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Sixty Angus × SimAngus-crossbred steers (body weight [BW] 279 ± 16 kg) were used to evaluate the effect of calcium salts of palm oil inclusion (CPO) and the amount of feed offered (AFO) on plasma glucose-dependent insulinotropic polypeptide (GIP) concentration and its association with energy metabolism and marbling score (MS) in feedlot steers. Steers were blocked by BW and gain to feed (G:F) and randomly assigned to individual feedlot pens. Treatments (2 × 2 factorial) consisted of ad libitum-fed steers without (ANF) or with (AWF) the inclusion of CPO or restricted-fed steers (85% of the ad libitum intake of ANF) without (RNF) or with the inclusion of CPO (RWF). After weaning, steers were adapted to individual pens and fed a corn silage-based diet for 30 d and subsequently placed in a ground corn (GC)-based diet. Diets were given ad libitum or at 85% of the ANF intake and with or without CPO. After 59 d on the finishing diet, all steers had ad libitum access to the finishing diet until harvest. Measurements of CO2 emission and O2 consumption to estimate respiratory quotient (RQ) were taken (n = 9/treatment). Correlations between plasma GIP and insulin concentrations and RQ were analyzed. A linear regression was performed to evaluate the association of plasma GIP and MS. All data were analyzed using the PROC MIXED procedure of SAS. During the first 103 d of the trial, there were AFO × CPO interactions (P ≤ 0.01) for BW, dry matter intake (DMI), average daily gain (ADG), and net energy for maintenance (NEm) intake. Ad libitum-fed steers without CPO presented the greatest DMI among dietary treatments and had greater BW and ADG compared with steers in the RWF and RNF treatments. After all steers had ad libitum access to dietary treatments, steers that were previously restricted showed a 30% and 19% increase (P ≤ 0.01) in ADG and G:F, respectively. There was a three-way interaction time × CPO × AFO (P = 0.04) for plasma GIP concentration. There was no correlation (P = 0.96) of GIP with RQ, whereas insulin demonstrated marginal significance for a positive (P = 0.07) and negative (P = 0.08) correlation with plasma GIP and RQ, respectively. There was no association (P = 0.30) between GIP and MS. These data indicate that GIP secretion results from an interaction between CPO and energy intake depending on the time relative to feed intake that GIP might indirectly regulate energy metabolism through insulin secretion, and that GIP does not appear to be associated with MS., (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Average temperatures in the Midwest, USA are predicted to increase 2-9°C by the end of the century; resulting in muddy pastures for spring calving beef heifers as they enter late gestation. The objective of this study was to evaluate the effects of muddy conditions on heifer body weight (BW), body condition score (BCS), conceptus free live weight (CFLW), and fetal growth when heifers were energy restricted during late gestation. Eighteen Angus heifers ( n  = 9/treatment) were paired based on initial BW. One heifer from each BW pair was randomly allocated to either the mud (MUD) or control (CON) treatment on day 196 of gestation. Pens in the CON treatment were bedded with wood chips, while pens in the MUD treatment were filled with mud (average depth of 19.5 ± 7.9 cm). Heifers were housed individually and fed the same diet that consisted of a limit-fed total mixed ration from day 196 to 266 of gestation that was formulated to meet 66% of the net energy for maintenance, growth, and gestation requirements. Requirements and the amount of the diet offered were adjusted weekly, and heifers were weighed and sampled for blood metabolites weekly. Data were analyzed as a randomized complete block design with repeated measurements. There was a treatment × day of gestation interaction, such that heifers had similar BW, BCS, and CFLW on day 196 of gestation. By day 266 of gestation; however, heifers in the MUD treatment weighed 43.5 kg less ( P  < 0.01) and were 1.8 BCS units less ( P  < 0.01) than heifers in the CON treatment. This is further supported by the treatment × day effects we observed for back fat (BF) and rump fat (RF) thickness, such that the MUD heifers had less BF ( P  = 0.02) and RF ( P  < 0.01) by day 266 of gestation. There was a marginally significant difference for gestation length ( P  = 0.06), such that heifers in the MUD treatment calved approximately 3.1 days before the heifers in the CON treatment. Though heifers in the MUD treatment decreased their BW and CFLW during the treatment period, we did not observe a difference in calf birth weight ( P  = 0.34), calf plasma IgG concentration ( P  = 0.37), or calf weaning weight ( P  = 0.63). Despite heifers in the MUD treatment having greater BW, CFLW, and BCS losses compared with the heifers in the CON treatment, the heifers in the MUD treatment seemed to prioritize fetal growth, as they mobilized their body tissues to meet the energetic demands of pregnancy., (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Glucose tolerance tests (GTT) are commonly performed in beef cattle to evaluate the glucose-insulin signaling pathway. Blood samples are obtained via a catheter and then transferred back to the laboratory for further analysis. A hand-held glucometer used chute-side can make performing GTT's and quantifying blood glucose concentration much easier and faster for research purposes. The purpose of this study was to evaluate the agreement between a hand-held electronic glucometer (Precision Xtra; Abbott Diabetes Care Inc., Mississauga, ON, Canada) for chute-side use in beef cattle compared with a colorimetric assay in the laboratory (Stanbio Glucose LiquiColor; Stanbio Laboratory, Boerne, TX, USA). A GTT was performed on 13 Simmental × Angus steers during the growing phase. Blood samples were obtained via a jugular catheter. Glucometer readings were taken immediately after blood was sampled from the jugular with no preservative, and laboratory measurements were conducted on plasma preserved with sodium fluoride. A paired t-test (P = 0.40), Pearson's correlation (P < 0.001; r = 0.95), Bland-Altman plot, and Lin's concordance correlation coefficient (LCCC = 0.90) were completed to evaluate the performance of the glucometer relative to the results from the laboratory assay. Based on the results, we conclude that the glucometer is an acceptable method for measuring blood glucose concentration in beef cattle under field conditions., Competing Interests: The authors have declared that no competing interests exist.

The objectives of this trial were to evaluate the association between different levels of dry matter intake (DMI) on gas exchange, plasma glucose-dependent insulinotropic polypeptide (GIP) concentration, and intramuscular (IM) fat deposition. We used 60 individually fed backgrounded Angus × SimAngus-crossbred steers ( n = 30) in a randomized complete block design. Steers (paired by body weight [BW] and gain to feed ratio [G:F]) were randomly allocated to one of the following treatments: ad libitum intake (AI) or restricted intake (RI; the same diet fed at 85% of the AI) of a finishing diet. The diet contained 61% cracked corn, 9% corn silage, 15% distillers' dried grains with solubles, 5% soyhulls, and 10% of a protein-mineral-vitamin premix. Measurements of CO 2 emission and consumption of O 2 , and respiratory quotient (RQ) were taken using the GreenFeed system ( n = 15/treatment). Plasma and gas samples were collected 10 d before slaughter, 1 h before and 2 h after feeding. Plasma glucose, non-esterified fatty acids, GIP, and insulin concentration and gasses (O 2, CO 2, and RQ) were analyzed using the MIXED procedure of SAS evaluating the fixed effect of treatment, time (repeated measurement) and their interaction, and the random effect of the block. Final BW and carcass characteristics were analyzed with a similar model, without the time statement and its interaction. Compared with RI, AI steers had greater ( P < 0.01) DMI and average daily gain (ADG). Steers on AI had greater final BW ( P = 0.02), tended to have a greater ribeye area ( P = 0.09), and had lower plasma GIP concentration ( P = 0.04). There was no treatment effect ( P ≥ 0.11) on G:F, subcutaneous backfat (BF), and IM fat, O 2 consumption, CO 2 emission, and RQ. Plasma glucose concentration of AI steers was greater before and after feeding than RI ( P < 0.05). In conclusion, feeding steers ad libitum increased DMI, ADG, and plasma glucose and GIP concentration but does not affect G:F, BF, IM fat, CO 2 emission, and O 2 consumption. Plasma GIP concentration and RQ are not associated with IM fat deposition., (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Electronic devices have been used to describe chemical compounds in the food industry. However, there are different models and manufacturers of these devices; thus, there has been little consistency in the type of compounds and methods used for identification. This work aimed to determine the applicability of electronic nose (e-nose) Cyroanose 320 to describe the differentiation of volatile organic compounds (VOCs) in fresh Mexican cheese (F-MC) formulated with milk from two different dairy cattle breeds. The VOCs were described using a device manufactured by Sensigent and Solid-Phase Micro-extraction (SPME) coupled to GC-MS as a complementary method. The multivariate principal components analysis (PCA) and the partial least squares discriminant analysis (PLS-DA) were used to describe the relationships of VOCs to electronic nose data, sensory data, and response levels. In addition, variable importance in projection (VIP) was performed to characterize the e-nose signals to the VOCs. The e-nose distinguishes F-MC prepared with milk from two dairy breeds. Sensor number 31 correlated with carboxylic acids most in F-MC from Jersey milk. The HS-SPME/GC-MS identified eighteen VOCs in F-MC made with Holstein milk, while only eleven VOCs were identified for F-MC made with Jersey milk. The more significant peaks in both chromatogram analyses were Propanoic acid, 2-methyl-, 1-(1,1-dimethylethyl)-2-methyl-1,3-propanediyl ester in cheese made from Holstein milk and Propanoic acid, 2-methyl-, 3-hydroxy-2,4,4-trimethylpentyl ester in Jersey milk cheese. Both compounds are considered essential carboxylic acids in the dairy industry. Thus, sensor 31 in the electronic nose Cyranose 320 increased its response by essential carboxylic acids identified by HS-SPME/GC-MS as a complementary method. The e-nose Cyranose 320 is potentially helpful for evaluating fresh Mexican cheese authentication independent of cows' milk samples from different breeds.

This research communication reports the relative abundance of fatty acids in mammary fat pad (MFP) and subcutaneous adipose (SCA) tissues for Holstein heifer calves receiving 0, 3, or 12 daily injections of estradiol immediately prior to tissue collection. The objective of this study was to determine if the MFP and SCA fatty acid profiles were affected by estradiol administration and if such a response differs between adipose tissue depots. Twelve Holstein heifer calves were reared on a common diet and administered 12 daily injections prior to euthanasia. Injections were either daily injections of corn oil (n = 4; CON), 9 injections of corn oil followed by 3 injections of estradiol (n = 4; SHORT), or 12 injections of estradiol (n = 4; LONG). Fatty acids were extracted from collected MFP and SCA tissues samples and analyzed using gas chromatography. The MFP tissues contained a greater abundance of saturated fatty acids than SCA tissues which complemented a reduced abundance of mono-unsaturated fatty acids in the MFP than SCA. Extended duration of estradiol administration increased the abundance of total omega 3 fatty acids in both MFP and SCA tissues. There was a treatment by tissue interaction for several of the C18:1 and C18:2 isomers indicating that estradiol's effects on fatty acid uptake and metabolism are tissue specific. Additionally, C18 uptake and metabolisms may have important roles in mammary growth and development. Together, these results indicate that the MFP responds differently to estradiol administration than SCA tissues and that these alterations are associated with different degrees of induced mammary growth via estradiol.

Mud increases net energy requirements for cattle because mud and precipitation compromise the ability of the hair coat to insulate and maintain core body temperature of the cow. The increase in energy required for a gestating cow to compensate for a muddy environment is unknown. The objective of this study was to evaluate effects of muddy conditions on cow body weight (BW) and fetal growth during late gestation. Sixteen multiparous Angus cows ( n = 8/treatment) were paired based on initial BW and one cow from each pair was randomly allocated to either the mud (MUD) or control (CON) treatment on day 213 of gestation. Pens in the CON group were bedded with wood chips, while pens in the MUD group were designed to create a muddy lot (average depth of 23.6 ± 5.8 cm). Cows were housed outdoors individually and fed the same diet that consisted of a limit-fed total mixed ration. Each pair was fed to meet energy and protein requirements for maintenance and gestation. From day 213 to 269 of gestation, cows were weighed and sampled for blood metabolites weekly. Data were analyzed as a randomized complete block design with repeated measurements (SAS 9.4). Though cows consumed the same amount of dry matter, cows in the MUD treatment weighed 37.4 kg less than cows in the CON treatment ( P < 0.01) by day 269 of gestation. Cows in the MUD treatment decreased approximately half a body condition score (BCS), while cows in the CON treatment gained approximately 1 BCS during the treatment period ( P < 0.01). There was no evidence of a treatment × day of gestation effect for 12th rib back fat ( P = 0.85), rump fat ( P = 0.48), total plasma protein concentrations ( P = 0.85), or plasma 3-methylhistidine ( P = 0.84); however, there was a marginally significant treatment × day of gestation effect for plasma non-esterified fatty acid concentration ( P = 0.09). Despite differences in cow BW at the end of the treatment period, calf birth weight ( P = 0.66) and calf total plasma protein ( P = 0.27) were not different; however, the divergence in cow BW remained marginally significant at parturition ( P = 0.06). These results indicate that mud increased net energy requirements for cows in the MUD treatment, as calf birth weight was not different but maternal BW was decreased compared with cows in the CON treatment., (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objective of the present experiment was to evaluate the effect of maternal supplementation with fatty acids (FAs) and methionine (Met) during late gestation on offspring growth, energy metabolism, plasma resolvin (RvD1) concentration, carcass characteristics, and hepatic mRNA expression. Ewes (5 pens/treatment; 3 ewes/pen) blocked by body weight (BW) were assigned to one of four treatments from day 100 of gestation until lambing. The treatments were: basal diet (NS) without FAs or Met supplementation; FA supplementation (FS; 1.01 % of Ca salts, containing n-3 FA); Met supplementation (MS; 0.1 % of rumen-protected methionine); and FS and MS (FS-MS). At birth (day 0), ewes and lambs were placed in a common pen. On day 60, lambs were weaned, sorted by sex, blocked by BW, and placed on a common finishing diet for 54 d (FP). A lamb per pen was used for a glucose tolerance test (GTT) after the FP. Carcass characteristics were recorded on day 56. Lamb data were analyzed as a randomized complete block design with a 2 × 2 × 2 factorial arrangement, with repeated measurements when needed (SAS 9.4). At weaning, lambs born to MS- or FS-fed ewes were heavier than lambs born from FS-MS ewes (FS × MS × Time; P = 0.02). A marginal significant FS × MS interaction (P = 0.09) was also observed on RvD1; lambs born to ewes in the NS and FS-MS treatments showed a lower RvD1 plasma concentration when compared with lambs born to FS- or MS-fed ewes. Lambs born to dams fed FA showed an increase (P = 0.05) in liver COX-2 mRNA relative expression. Lambs born to ewes supplemented with Met showed an increase (P = 0.03) in liver FABP4 mRNA expression. An FS × MS × Time interaction (P = 0.07) was observed in plasma glucose during the GTT; lambs born from FS-fed ewes showed lower plasma glucose concentration than lambs born to Met-supplemented ewes at 2 min after bolus administration. During the GTT, a marginal significant effect (P = 0.06) was observed for the lamb average insulin concentration due to maternal Met supplementation during late gestation, where these lambs had the lowest plasma concentration. Contrary to our hypothesis, the interaction of FA and Met supplementation during late gestation did not show a greater positive effect on offspring postnatal growth and metabolism. However, the individual supplementation of each nutrient has an effect on offspring development with a concomitant change in markers involved in the inflammatory response and energy metabolism., (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. 
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One-hundred twenty crossbreed steers (initial body weight (BW) 566 ± 42 kg) were used to evaluate the interaction of protein source (PS) and nonroughage NDF content (NRFC) in finishing diets of feedlot cattle fed free-choice hay on performance and carcass characteristics. Steers were stratified by BW and randomly assigned to 8 pens (2 × 2 factorial) and fed for 104 ± 10 d. Four dietary treatments were investigated: (1) distillers' dry grains with solubles (DDGS) and a low NRFC (DLF), (2) DDGS and a high NRFC (DHF), (3) soybean meal (SBM) and a low NRFC (SLF), (4) SBM and a high NRFC (SHF). Free-choice grass hay and concentrates were offered in a different bunk. Data were analyzed as a randomized complete block design. Do to the confounded effect of PS and protein intake, a linear regression was used to evaluate the effect of protein intake in growth performance. For gain to feed ratio (G:F) an interaction tended to occur ( P = 0.10) between PS and NRFC. Steers on the DHF treatment had a lower G:F compared with SLF and SHF. Feeding SBM increased ( P = 0.05) final BW, tended to increase ( P = 0.06) average daily gain (ADG), and decreased ( P = 0.05) hay intake (HI) compared with steers fed DDGS. There was a positive association ( P ˂ 0.01) of crude protein intake with ADG and FBW. Dietary NRFC did not change ( P ≥ 0.3) final BW, ADG, DMI, and HI. Protein source did not affect ( P ≥ 0.16) hot carcass weight (HCW), longissimus muscle (LM) area, dressing, 12th rib fat thickness, or marbling score (MS). No differences were detected between NRFC for dressing, HCW, LM area, or MS ( P ≥ 0.18); but diets with greater NRFC decreased ( P = 0.03) the 12th rib fat thickness. Steers in the SHF treatment presented the lesser kidney-pelvic-heart fat compared with the remaining treatments (PS × NRFC interaction, P = 0.04). Soybean meal inclusion/increase in protein intake improved growth performance of feedlot steers compared with DDGS, despite protein intake meet the protein requirement. Increasing the NRFC did not affect growth or HI but decreased feed efficiency of steers fed DDGS., (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The purpose of this study was to evaluate the effect of intranasal oxytocin administered at abrupt weaning on weaning behaviors such as walking distance and time devoted to walking, calf body weight, and plasma non-esterified fatty acids (NEFAs), β-hydroxybutyrate (β-HB), and cortisol. Twenty Simmental × Angus heifer calves were randomly assigned to one of two treatments: intranasal oxytocin (OXT; n = 10) or intranasal saline (CON; n = 10). All calves were given the respective intranasal treatment on the day of weaning (day 0) and then placed on pasture together. Calves were weighed and a blood sample was obtained on days 0, 1, 7, and 14 postweaning. Blood samples were subsequently used to quantify plasma NEFA, β-HB, and cortisol concentrations. All calves in both treatment groups were fitted with an individual global positioning system that recorded calf location every 10 s for a 16-h period on days 0, 7, and 14 to quantify and evaluate walking behaviors. There was no treatment × day effect for distance walked ( P = 0.82), walking time ( P = 0.80), non-walking time ( P = 0.88), area utilization index ( P = 0.84), calf body weight ( P = 0.82), average daily gain ( P = 0.54), NEFA ( P = 0.22), or cortisol concentrations ( P = 0.32). There was a tendency for a treatment × day effect observed for average walking speed ( P = 0.09) and β-HB ( P = 0.10), such that calves in the CON treatment tended to have lesser average walking speeds on day 14 and tended to have greater β-HB concentration after weaning. There was a treatment effect ( P = 0.02) for NEFA concentrations, with the CON calves having a greater plasma NEFA concentration throughout the study compared with OXT calves. These data imply that OXT calves may have had differing metabolic responses immediately after weaning that could have altered the mobilization of NEFA, but this change was not substantial enough to impact body weights or walking behaviors., (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Ghrelin is a gut hormone related to energy balance and reproductive functions. The aim of this study was to evaluate the effect of ghrelin antagonist D-Lys3-GHRP-6 (GA) as a potential agent that prevents ghrelin effects during bovine oocyte maturation on progesterone production, cumulus cell (CC) viability, CC DNA damage and embryo development and hatching rates. Ghrelin's potential to induce oxidative stress in cumulus-oocyte complexes (COC) was also evaluated. COCs were cultured for 24 hr in medium without supplementation (C) or supplemented with 60 pM ghrelin (Ghrelin60), Ghrelin60 + 20 pM GA (GA20), Ghrelin60 + 60 pM GA (GA60) or Ghrelin60 + 100 pM GA (GA100) for experiment I. For experiment II, C and Ghrelin60 treatments were used. Differences between C and Ghrelin60 and the linear or quadratic association between GAs on Ghrelin60 were evaluated. Results demonstrated that Ghrelin60 increased progesterone concentration, reduced CC viability, induced CC DNA damage and decreased blastocyst and hatching rate compared with C (p < .05). GA20, GA60 and GA100 had a linear effect on CC genetic damage index (p ≤ .05) and a quadratic effect on CC viability (p < .01). GA20 counteracted the low hatching rate produced by Ghrelin60. However, GAs did not counteract progesterone concentration and blastocyst rate (p ≥ .21). GRH60 did not differ from C in the oxidative status (p ≥ .19). Our study highlights that GA could prevent the negative effects of ghrelin during bovine IVM., (© 2021 Wiley-VCH GmbH.)

In mammals, including sheep and mice, lactation attenuates the hypothalamo-pituitary-adrenal axis and plasma cortisol concentration. Oxytocin, one neuropeptide present in the blood during lactation, may contribute to such stress attenuation. Providing oxytocin intra-nasally increases plasma oxytocin concentration in cattle and can be used in non-lactating cows to mirror plasma oxytocin concentration of lactating cows. Therefore, our hypothesis was that there would be no difference in plasma cortisol between non-lactating beef cows intra-nasally administered oxytocin and lactating beef cows intra-nasally treated with saline. Twenty Bos taurus cows were randomly allocated by lactational status to one of four treatments, in a 2×2 factorial arrangement: 1) Non-lactating, saline (NL-S; n = 5); 2) Non-lactating, oxytocin (NL-OXT; n = 5); 3) Lactating, saline (L-S; n = 5); and 4) Lactating, oxytocin (L-OXT; n = 5). Two hours pre-treatment, cows were catheterized, moved to their appropriate chute and baseline blood samples were collected at -60, -45, -30, and 0 minutes before treatments were administered. Directly following the 0-minute sample, cows were administered their intra-nasal treatment via a mucosal atomization device. Subsequently, blood was collected at 2, 4, 6, 8, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, and 120 minutes. Non-lactating cows had greater (P = 0.02) plasma cortisol concentration compared with lactating cows. There was no lactation by treatment interactions for either plasma cortisol (P = 0.55) or oxytocin (P = 0.89) concentration. Although a treatment by time interaction was identified for oxytocin (P < 0.0001), there was no main effect of lactation on plasma oxytocin concentration (P = 0.34). Similar oxytocin and dissimilar cortisol concentration in lactating and non-lactating cows indicate that oxytocin alone cannot be responsible for reduced plasma cortisol in lactating ruminants. Further investigations are needed to elucidate alternative mechanisms that may be involved in the stress hypo-responsive condition of lactating mammals., Competing Interests: The authors have declared that no competing interests exist.

The objective of this experiment was to evaluate the effects of feeding different levels concentrations of dietary calcium propionate (CaPr) on lambs' growth performance; ruminal fermentation parameters; glucose-insulin concentration; and hypothalamic mRNA expression for neuropeptide Y (NPY), agouti-related peptide (AgRP), and proopiomelanocortin (POMC). Thirty-two individually fed lambs were randomly assigned to four treatments: (1) control diet (0 g/kg of CaPr), (2) low CaPr, (30 g/kg dry matter (DM)), (3) medium CaPr, (35 g/kg DM), and (4) high CaPr (40 g/kg DM). After 42 days of feeding, lambs were slaughtered for collecting samples of the hypothalamus. Data were analyzed as a complete randomized design, and means were separated using linear and quadratic polynomial contrast. Growth performance was not affected ( p ≥ 0.11) by dietary CaPr inclusion. The ruminal concentration of total volatile fatty acids (VFA) increased linearly ( p = 0.04) as dietary CaPr increased. Likewise, a linear increase in plasma insulin concentration ( p = 0.03) as dietary CaPr concentration increased. The relative mRNA expression of NPY exhibited a quadratic effect ( p < 0.01), but there were significant differences in the mRNA expression of AgRP and POMC ( p ≥ 0.10). Dietary calcium propionate did not improve lamb growth performance in lambs feed with only forage diets. Intake was not correlated with feed intake with mRNA expression of neuropeptides.

Supplementation with omega-3 and omega-9 fatty acids (FA) during late gestation regulates offspring development; however, their effect in the first third of gestation is unknown in sheep. The objective of this experiment was to evaluate the effects of the maternal supplementation with an enriched source of monounsaturated FA (MUFA) or an enriched source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) during the first third of gestation on productive performance on ewes and offspring, and hypothalamic neuropeptides on offspring. Seventy-nine post-weaning lambs, born of sheep supplemented in the first third of gestation with 1.61% Ca salts rich with MUFA or EPA+DHA (dam supplementation, DS), were distributed in a 2×2 factorial arrangement of treatments to finishing diets containing 1.48% of Ca salts of MUFA or EPA+DHA (lamb supplementation, LS). The finishing period of the offspring lasted for 56 d. During the finishing period dry matter intake (DMI, daily) and body weight (BW) were recorded. Plasma was collected for metabolites analysis. Twenty-four lambs were slaughtered, and hypothalamus was collected for mRNA expression of hormone receptors, neuropeptides, and lipid transport genes. The data were analyzed with a mixed model in SAS (9.4) using repeated measurements, when needed. There was a DS×LS interaction for BW (P = 0.10) where LS with EPA+DHA born from DS with MUFA were heavier than the other 3 treatments. Lambs born from DS with MUFA have a greater DMI (P < 0.01) than the offspring born from DS with EPA+DHA. Lambs born from MUFA supplemented dams had a greater (P ≤ 0.05) hypothalamus mRNA expression for cocaine and amphetamine regulated transcript, growth hormone receptor, metastasis suppressor 1, leptin receptor, pro-opiomelanocortin, and Neuropeptide Y. These results indicate that growth depends not on the type of FA during the finishing phase but the interaction of different sources of FA ad different stages. Also, supplementation with FA during early pregnancy changes productive performance and neuropeptides' mRNA expression of lambs independently of the finishing diet., (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Nutrition plays a critical role in developmental programs. These effects can be during gametogenesis, gestation, or early life. Omega-3 polyunsaturated fatty acids (PUFA) are essential for normal physiological functioning and for the health of humans and all domestic species. Recent studies have demonstrated the importance of n-3 PUFA in ruminant diets during gestation and its effects on pre-and postnatal offspring growth and health indices. In addition, different types of fatty acids have different metabolic functions, which affects the developmental program differently depending on when they are supplemented. This review provides a broad perspective of the effect of fatty acid supplementation on the developmental program in ruminants, highlighting the areas of a developmental program that are better known and the areas that more research may be needed.

Lambs born from dams supplemented with different sources of fatty acids (FA) during late gestation have a different growth rate and plasma glucose concentration. The main objectives of this experiment were to evaluate the effect of supplementing different sources of FA during late gestation on offspring plasma metabolite concentrations, growth, and on a glucose tolerance test (GTT) during the finishing phase. Fifty-four lambs (18 pens, 3 lambs/pen) were born from ewes supplemented during late gestation with one of three treatments: (1) no FA (NF); (2) a source of monounsaturated FA (PDS, 1.01% of Ca salts); or (3) a source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (EDS, 1.01% of Ca salts containing). At birth (day 0), supplementation ceased, and all ewes and lambs were placed in a common pen. On day 60, lambs were weaned, grouped by sex, blocked by body weight (BW), and placed on a common finishing diet for 54 days (FP). One lamb per pen was used for the GTT after the FP. There was a tendency for FA × Sex × Day interaction ( p = 0.08) on lamb growth during the finishing period, with PDS females being heavier than PDS males, while EDS males were heavier than EDS females at day 60. There was a tendency for FA × Sex interaction ( p = 0.06) for plasma insulin concentration for the GTT. Plasma insulin concentration of wethers increased as FA unsaturation degree increased during the GTT; the opposite happened with the plasma insulin concentration of female lambs. In conclusion, FA supplementation during late gestation tended to modified growth and insulin response to a GTT; these changes differed with the degree of FA unsaturation of the supplement and lamb sex.

Weaning and transport represent a high stress time for calves. Preconditioning ( PC ) by weaning before the transport separate these stressors. The stressors generate oxidative stress, which can be reduced by mineral and vitamin supplementation ( MVS ) with an antioxidant capacity. Our objective was to evaluate the effect of PC and MVS on performance of steers. The experiment used a 2 × 2 factorial arrangement design, considering a 26-d PC treatment from weaning to transport to the feedlot (day 0), and injectable MVS on days -45, -26, and 0. The MVS consisted of Cu, Zn, Mn, Se, vitamin E (0.2, 0.8, 0.2, 0.1, and 1 mg/kg body weight [ BW ], respectively), and vitamin A (1,190 IU/kg). Sixty Angus-crossbred steers (186.4 ± 27.6 kg) were randomly assigned to the four treatments ( MVS+PC ; N + PC ; MVS + N ; N + N ; n = 15 per treatment). BW was recorded on days -45, -26, 0, 8, 15, and 29. On day 0, an additional BW was taken 30 min after the 5-h transportation (day 0.5). Between days 0 and 29, dry matter intake ( DMI ) and average daily gain ( ADG ) to DMI ratio ( G:F ) were measured. Between days -26 and 29 plasma concentrations of glucose, nonesterified fatty acids ( NEFA ), cortisol, insulin, total antioxidant status ( TAS ), and thiobarbituric acid-reactive substances were evaluated. Data were analyzed using the MIXED procedure of SAS with repeated measures, using treatment, time, and treatment × time as fixed effects and steer as a random effect. Between days -26 and 0, there was an interaction of MVS × PC ( P ˂ 0.01) for ADG. From days -26 to 0, N+N and N+PC had the greatest and lesser ADG, respectively. On day 0.5, no-PC steers tended to lose BW, whereas the PC steers tended to gain BW ( P = 0.09). In the period days 0 to 8, there were no differences ( P ≥ 0.27) in DMI, but the PC steers had greater G:F and ADG ( P < 0.01) compared with no-PC steers. Plasma NEFA concentration on day 0 was affected by MVS × PC ( P < 0.01) because MVS decreased plasma NEFA concentration in no-PC steers, but it increased in the PC steers. Plasma concentrations of glucose, insulin, and cortisol did not differ among treatments ( P ≥ 0.23). There was an MVS × PC interaction ( P = 0.09) for TAS on day 0; N+N had the greatest TAS concentrations and MVS+N had the lowest TAS concentrations. In conclusion, a 26-d PC decreased steers BW compared with no-PC steers. The BW loss during PC was not recovered 29 d after feedlot entry. Despite this BW loss, MVS treatment decreased BW loss in the steers allocated to PC treatment on the day of transport., (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objectives of this trial were to evaluate the effect of corn processing during the finishing phase on feedlot cattle performance and carcass characteristics. We hypothesized that steers fed dry-rolled corn would have better feed efficiency (less feed necessary to the same amount of gain) than steers fed whole shelled corn. Ninety-five backgrounded Angus-cross cattle (initial body weight [BW] = 263 ± 9.8 kg) were used in a randomized complete block design in a feedlot setting. Cattle were divided in to 3 blocks: heifers (n = 31, 4 pens; initial BW = 267 ± 1.3 kg), light steers (n = 32, 4 pens; initial BW = 253 ± 1.3 kg), and heavy steers (n = 32, 4 pens; initial BW = 279 ± 1.4 kg). Diets contained 70% corn (experimental treatment), 15% dried distiller's grains with solubles, 7% grass hay, and 8% supplement, on a dry matter basis. Cattle were fed for 217, 224, and 231 ± 8 d for the heifer, heavy steer, and light steer blocks, respectively. Two pens within each block were randomly assigned to one of the following treatments: (1) dry-rolled corn (DRC) or (2) whole-shelled corn (WSC). Animal growth performance and carcass characteristics were analyzed using the MIXED procedure of SAS, including the fixed effect of treatment. Pen and block were included as random effects. Carcass characteristics USDA Yield Grade and Quality Grade distributions were compared using the GLIMMIX procedure of using the same model than the growth performance data. Data are presented as LSMeans. Cattle fed DRC had greater average daily gain (ADG; P = 0.02) and final BW ( P < 0.01) when compared with cattle fed WSC. Even though ADG was greater for the DRC treatment, feed efficiency was similar ( P = 0.45) because dry matter intake was also greater ( P = 0.04) for DRC when compared with the WSC treatment. Cattle fed DRC also had greater hot carcass weight ( P < 0.01), dressing percentage ( P < 0.01), and Longissimus dorsi muscle area ( P < 0.01) than cattle fed WSC. No differences ( P ≥ 0.18) were observed for marbling score, USDA Yield Grade or USDA Quality Grade. In conclusion, feeding DRC to feedlot cattle improved ADG, hot carcass weight, and Longissimus dorsi muscle area, without affecting feed efficiency or meat quality., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objective of the present study was to assess the association of prepartum lying time (LT) and the coefficient of variation (CV) of LT within 7 d before calving with prepartum serum nonesterified fatty acid (NEFA) concentration at 7 ± 3 d prepartum (dpp) and stillbirth. Prepartum pregnant Holstein heifers and cows from 3 dairy herds were used (n = 1,044). Animals were housed in freestall barns using a prepartum pen 21 d before the expected calving date and were moved into a contiguous maternity pen at parturition. Monthly, cohorts of 20 to 36 animals (heifers and cows combined) were enrolled at each farm and electronic data loggers (IceQube, IceRobotics, Edinburgh, UK) were fitted to the hind leg of individual animals to assess their behavioral activity. Stillbirth was defined as a calf born dead or died during the first 24 h after parturition in dams with normal gestation length. The LT was recorded for the last 7 dpp to assess differences among dams with stillbirth versus those with a calf born alive. Mean LT within 7 d before blood NEFA collection was assessed to determine the association with prepartum serum NEFA at 7 ± 3 dpp. Blood samples for the assessment of serum NEFA concentration were collected from prepartum animals at 14 ± 3 and at 7 ± 3 dpp. Blood samples for total serum calcium concentration were collected from postpartum cows within 48 h after parturition to assess differences among cows with stillbirth versus those with a calf born alive. Data were analyzed using CORR, MIXED, or GLIMMIX procedures of SAS (SAS Institute Inc., Cary, NC). Dams experiencing dystocic births had a greater proportion of stillbirth, but herd, parity, and season did not have an effect. Dams with a stillborn calf had reduced LT and increased CV of LT within the last 7 dpp compared with dams with a calf born alive, regardless of parity. Multiparous cows with a stillborn calf had higher prepartum serum NEFA concentration compared with multiparous cows with a calf born alive, but this did not differ for first-calf heifers. Regardless of parity, the proportion of postpartum cows with hypocalcemia was higher for dams with a stillborn calf compared with those with a calf born alive. Regardless of parity, LT of prepartum dams was strongly correlated with the CV of LT (as LT increased, the CV decreased), and prepartum dams with a mean LT between 11 and 15 h/d had the lowest serum NEFA concentration compared with dams with LT of 8 to 10 or >16 h/d. Serum NEFA concentrations at 7 ± 3 dpp was positively correlated with the CV of LT within 7 d before blood sample. These results show that the dam's prepartum LT and its consistency over time are associated with prepartum serum NEFA and calf survival at calving., (Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Background: Omega-3 PUFA or methionine (Met) supply during gestation alters offspring physiology. However, the effect of both nutrients on fetal development has not been explored. Our objective was to determine the effects of supplementation of these two nutrients during late gestation on fetal growth, DNA methylation, and mRNA expression of genes associated with the inflammatory response, and DNA methylation. Ewes (n = 5/treatment) were fed from day 100 to 145 of gestation one of the following treatments: 1) basal diet (NS) without fatty acids (FS) or methionine (MS) supplementation; 2) FS (10 g/kg Ca salts, source omega-3 PUFA); 3) MS (1 g/kg rumen protected methionine); and 4) FS and MS (FS-MS). On day 145, ewes were euthanized, and data from dams and fetus was recorded. Placenta (cotyledon), fetal liver, and blood samples were collected., Results: A treatments interaction on fetal liver weight, ewe body weight and body condition score (BCS) was observed; FS-MS were heavier (P <  0.01) than FS and MS, and FS-MS ewes had a better (P = 0.02) BCS than NS. Methionine increased (P = 0.03) ewe plasma glucose concentration. Fetal liver global DNA methylation increased (P <  0.01) in FS and MS. Dietary treatments modify the mRNA relative expression on some of the genes evaluated. In the fetal liver, FS increased (P = 0.04) the mRNA relative expression of arachidonate-5-lipoxygenase-activating-protein and tended to decrease (P = 0.06) methionine-adenosyltransferase-1A. Moreover, MS decreased (P = 0.04) DNA-methyltransferase-1 and tended to decrease (P = 0.08) free-fatty-acid-receptor-1 mRNA relative expression. Furthermore, FS-MS decreased mRNA relative expression of tumor-necrosis-factor-alpha (P = 0.05), peroxisome-proliferator-activated-receptor-delta (P = 0.03) and gamma (P = 0.04), tended to decrease (P ≤ 0.09) interleukin-6, fatty-acid-transport-protein-1, and delta-5-desaturase, and increased adenosylhomocysteinase (P = 0.04) mRNA relative expression. In cotyledon, FS tended to decrease fatty acid binding protein 4 (P = 0.09) mRNA relative expression., Conclusion: Omega-3 PUFA and Met supplementation improves dam's performance in late gestation, which was positively correlated with an increase in offspring's liver development. Moreover, FS-MS decreased mRNA relative expression of proinflammatory cytokines, and lipogenic genes, and increased the expression on an enzyme that has an important role in methylation.

A negative energy balance (NEB) is detrimental to reproduction in animals. A suggested link between NEB and reproductive failure is the gastrointestinal hormone ghrelin, because of the association between ghrelin and the hypothalamo-pituitary-gonadal axis. The [D-Lys3]-Growth Hormone Releasing Peptide-6 ([D-Lys3]-GHRP-6) is a ghrelin antagonist that acts on ghrelin receptors (GHS-R1). The objective of this study was to evaluate the effect of [D-Lys3]-GHRP-6 on reproduction variables in feed restricted ewes. Two experiments were conducted. Experiment I was conducted for 30 days; and Experiment II for 13 days. In both experiments the ewes (n = 18) were randomly assigned to: Control (CO): fed to meet maintenance requirements; Feed restriction (FR): 80% of maintenance restriction; or Ghrelin antagonist (GA): feed restricted and daily subcutaneous of 7.5μg/kg of [D-Lys3]-GHRP-6. Plasma was collected to measure hormones and metabolite concentration. In Experiment II, the hypothalamus and ovaries were collected on day 13. In both Experiments, sheep allocated to the FR and GA treatments decreased their body weight compared with sheep in the CO group (P < 0.06); progesterone however, did not differ between treatments (P > 0.10). Experiment I: Plasma ghrelin concentration was greater (P < 0.01) in FR and GA compared with CO ewes. Plasma non-esterified fatty acids concentration was greater (P < 0.01) in GA and FR than CO. Experiment II: Kisspeptin1-Receptor (Kiss1-R) mRNA expression was greater in FR (P < 0.01) and tended to be greater in GA (P = 0.10) compared with CO ewes. The neuro peptide-Y (NPY) mRNA expression was greater (P = 0.03) in FR than CO; and tended to be greater (P = 0.06) compared with GA ewes. Growth hormone releasing hormone (GhRH) mRNA expression was greater in GA (P = 0.04) and tended to be greater in FR (P = 0.07) compared with CO ewes. Feed restriction increased GhRH, NPY, and Kiss-R mRNA expression in hypothalamus without affecting reproductive variables.Ghrelin antagonist may prevent an increase inNPY expression in ewes., Competing Interests: The authors have declared that no competing interests exist.

Herbal formulas during pregnancy have been used in developing countries. Despite that, the potential effects on the mother and offspring and whether those supplements elicit epigenetic modifications is still unknown. Therefore, our objectives were to determine the effects of supplemental herbal choline source (BCho) on the percentage of 5-hmC in whole blood from gestating ewes and their offspring, as well as determining the milk quality and growth of the offspring. Thirty-five gestating Rambouillet ewes were randomly assigned to five treatments: T1, supplementation of 4 g per day (gd -1 ) of BCho during the first third of gestation; T2, supplementation of 4 gd -1 of BCho during the second third of gestation; T3, supplementation of 4 gd -1 of BCho during the last third of gestation; T4, supplementation of 4 gd -1 of BCho throughout gestation; and T5, no BCho supplementation (control). For the 5-hmC DNA analysis, whole blood from ewes was sampled before pregnancy and at each third of gestation (50 days). Whole blood from lambs was sampled five weeks after birth. The evaluation of the nutritional programming effects was conducted through the percentages of 5-hmC in the lambs. Compared with other treatments, the whole blood from ewes supplemented during T1 and T4 had the greatest 5-hmC percentages ( p < 0.05). However, only ewes fed BCho throughout gestation (T4) maintained the greatest percentages of 5-hmC ( p < 0.05). The lamb growth performance indicated that the BCho maternal supplementation did not affect the nutritional programming. However, the lambs born from ewes supplemented during T2 had the greatest 5-hmC percentages ( p < 0.05). Our data suggest that ewes supplemented during T4 with BCho increase and maintain the percentages of 5-hmC in whole blood, and the offspring born from ewes supplemented with BCho during T2 maintained the greatest percentages of 5-hmC 35 d after they were born.

Increased intestinal permeability can be observed during the physiologic stress response and has been linked to suppression of animal health and performance. Previously published data have shown the efficacy of fluorescein isothiocyanate dextran (FITC-d; 4.17 mg/kg) as a marker of enteric inflammation and mucosal barrier function in multiple species. Fluorescein is a smaller, less expensive alternative molecule possessing similar properties. The following two experiments compared FITC-d and fluorescein as potential indicators of intestinal permeability in pre- and postweaned lambs administered daily intramuscular injections of dexamethasone (Dex; 0.1 mg/kg) for 1 wk. Experiment 1 consisted of five preweaned lambs that were placed in one of two treatment groups: fluorescein with Dex (F+Dex) or fluorescein only (F). On day 7, blood was collected before and 1 h after oral administration of fluorescein (50 mg/kg). Experiment 2 included 12 weaned lambs and four treatment groups: F+Dex, F, FITC-d with Dex (Fd+Dex), and FITC-d only (Fd). On day 7, blood was collected before and 2 h after oral administration of FITC-d (4.17 mg/kg) or fluorescein (50 mg/kg). Plasma fluorescence was reported as the ratio between T1h/T0 or T2h/T0 for experiment 1 or 2, respectively. Experiment 1 showed a significant increase in T1h/T0 ratio of F+Dex relative to F lambs (P = 0.05) indicative of increased leaky gut; however, no differences (P = 0.22) were obtained in experiment 2. Results of these experiments suggest fluorescein may serve as a suitable marker of enteric permeability in preruminant lambs, but not in those with functional rumens., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The objectives of this experiment were to evaluate the effect of oscillating feeding time (OFT) and corn processing (CoP) on performance and carcass characteristics of feedlot cattle. One hundred sixty-five steers (initial body weight [BW] 277.2 ± 27.80 kg) were blocked by initial BW and allotted to 24 pens. Pens within each block were randomly assigned based on a 2 × 2 factorial arrangement of treatments. The two factors were CoP (whole shelled corn vs. ground corn [GC]) and feeding time (FT) (constant FT vs. 2 hours OFT). Animals were fed the same diet, only changing the CoP method depending on the treatment. Feed offered and feed refusals were collected daily. Body weight was collected at starting day of the experiment (d1) and every 28 d until the end of the experiment. At the end of the experiment, animals were harvested in a commercial slaughter facility, and carcass data were collected by a USDA grader. Animal growth performance and carcass characteristics data were analyzed with the PROC Mixed procedure (SAS) using CoP, FT, and their interaction as fixed variables; and treatments × pen within each block and block were considered random variables. There was no CoP by FT interaction differences ( P > 0.10) on animal growth performance variables, nor on hot carcass weight, back fat, rib eye area, or the percentage of kidney, pelvic, and heart fat. There was a tendency for an interaction ( P = 0.08) for marbling score, where the steers from the GC processing fed on the oscillating time had a lesser marbling score than the other three treatments. Oscillating feeding time improved ( P = 0.05) average daily gain; but did not affect ( P ≥ 0.11) dry matter intake (DMI), nor carcass characteristics compared with feeding at a similar time every day. There were no effects ( P ≥ 0.11) of CoP on growth performance, nor carcass characteristics. In conclusion, a 2-hour oscillation in FT might not decrease steer feedlot performance. This may be due to the high degree of control of DMI with feed bunk management., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science.)

In sheep, polyunsaturated fatty acid (PUFA) supplementations in late gestation increases the growth of offspring; however, there is a lack of evidence on the effect of PUFA supplementation during early gestation. Thus, the objective of this study was to evaluate the effect of dietary supplementation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in early gestation pregnant ewes on fatty acid concentration of fetal liver (FL) and fetal central nervous system (FCNS), and relative abundance of the mRNA for genes associated with transport and metabolism of fatty acids in FL and placenta. A total of 12 ewes, block for stage of gestation were fed a diet containing 1.6% (dry matter basis) monounsaturated fatty acids (MUFA) or EPA+DHA during the first 45 days of gestation. A cesarean section was conducted on day 45 of gestation to collect placenta (caruncle and cotyledon), FL, and FCNS. Relative abundance of mRNA in FL and FCNS and fatty acid concentration were analyzed using a 2x2 factorial arrangement of treatments considering fatty acid supplementation and tissue as the main factors. Concentrations of C18:1 isomers increase (P < 0.05) in FL and FCNS with MUFA supplementation; the FL and FCNS had a greater concentration of C20:3(n-6), C20:3(n-3), C22:1, C22:5 and C22:6 (P < 0.05) with EPA+DHA supplementation. In FL, the relative abundance of LPL mRNA was greater (P = 0.02) as a result of MUFA supplementation. In placenta, there was a FA x tissue interaction for relative abundance of DNMT3b and FFAR-4 mRNA (P < 0.05). Fetus from MUFA-supplemented dams had a greater relative abundance of FABP-4 mRNA (P < 0.05). Results indicate supplementation with EPA+DHA during early gestation increases the total EPA and DHA in FL. For the placenta, EPA+DHA supplementation led to an increase in the relative abundance of lipid mRNA for transport genes., Competing Interests: The authors have declared that no competing interests exist

Providing the neuropeptides oxytocin and vasopressin intranasally increased concentrations in plasma and cerebral spinal fluid in humans and primates, respectively. This is of interest because of the documented anxiolytic effects of oxytocin observed in humans and rodents. To date, a transnasal approach of hormone administration has not been investigated in beef cattle. Defining the pharmacokinetics of intranasal oxytocin in cattle is necessary for determining optimum sampling and dosing timelines for future investigations. Five, weaned Bos taurus steers were used in a 3 × 3 Latin square design. Treatments included 1) 0.33 IU oxytocin/kg BW (A, n = 5), 2) 0.66 IU oxytocin/kg BW (B, n = 5), and 3) 1.32 IU oxytocin/kg BW (C, n = 5). Steers were acclimated to handling and restraint procedures for 4 wk leading up to the start of the experiment. Frequent blood collection occurred every 2 min for the first 30 min and every 5 min for the second 30 min, relative to administration of intranasal treatment. No treatment by time interaction was detected; however, there was an effect of time (P < 0.001) and treatment (P = 0.002) on oxytocin concentrations over time. Pharmacokinetic parameters, determined by PKSolver excel add-in, demonstrated an average maximum concentration (C MAX ) of 63.3 pg/mL at 3.5 min after intranasal dose administration. An average half-life (T 1/2 ) of 12.1 min after intranasal administration was determined. Pharmacokinetic parameters to a single bolus were not dose-dependent., (Copyright © 2019 Elsevier Inc. All rights reserved.)

The U.S. Department of Agriculture (USDA) yield grade (YG) equation is used to predict the retail yield of beef carcasses, which facilitates a more accurate payment for cattle when they are sold on a grid pricing system that considers carcass composition instead of body weight alone. The current USDA YG equation was developed over 50 yr ago. Arguably, the population of cattle used to develop the YG equation is different than the current diverse U.S. beef cattle supply today. The objectives of this manuscript are to promote the adoption and use of precision agriculture technologies (i.e., camera grading and electronic animal identification) throughout the U.S. beef supply chain as a means to enhance the ability of the USDA YG equation to more accurately predict the retail yield across the population of cattle that contributes to the current U.S. beef supply. Camera grading has improved the accuracy of determining beef carcass retail yield; however, the use of electronic animal identification would allow for additional information to be passed back and forth between the packer, cattle feeder, and producer. Information, such as sex, genetics, medical treatment history, diets consumed, and growth promotant administration, as well as other information could be used to create additional variables for a new augmented USDA YG equation. Herein, fabrication yields demonstrated a 5.6 USDA YG and 12.8% boneless closely trimmed retail cut difference between actual cutout measurements and calculated values from the USDA YG equation for Jersey-influenced cattle. Evidence of such disparities between calculated and actual values warrants a reevaluation of the USDA YG system and consideration for implementing advancements in precision agriculture to improve the prediction of beef carcass retail yield to more accurately account for the large amount of variation in beef carcass retail yield from the cattle in the United States., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objectives of this trial were to evaluate the association between corn processing, glucose-dependent insulinotropic polypeptide (GIP) concentration, and intramuscular (IM) fat deposition. We hypothesized that steers fed whole shelled corn (WSC) would have a greater IM fat deposition than steers fed cracked corn (CC) due to an increase in plasma GIP concentration. Backgrounded, Angus-cross cattle (initial body weight [BW] = 279 ± 9.8 kg) were used in a randomized complete block design in a feedlot setting for an average of 230 d. Cattle were allotted in 12 pens (6 pens per treatment with 8 animals per pen). There were three blocks: heifers ( n = 32, initial BW = 265 ± 1.3 kg), small steers ( n = 32, initial BW = 262 ± 1.3 kg), and large steers ( n = 32, initial BW = 310 ± 1.4 kg). Two pens within each block were randomly assigned to one of the following treatments: 1) CC or 2) WSC. Animal growth performance, carcass characteristics, and plasma hormone and metabolite concentrations were analyzed using the MIXED procedure of SAS, including the fixed effects of treatment, or treatment, time, and their interaction. Pen and block were included as random effects. Carcass yield and quality grade distributions were compared using the GLIMMIX procedure of SAS. including the fixed effects of treatment and time with pen and block as random effects. Linear regression was used to evaluate the association of plasma GIP concentration and IM fat content. Average daily gain ( P = 0.57) and final BW ( P = 0.34) were similar, regardless of treatment. Cattle fed CC had reduced ( P < 0.01) dry matter intake (DMI) when compared with those fed WSC. This lesser DMI resulted in improved gain:feed ratio ( P < 0.01) for cattle fed CC compared with cattle fed WSC. There was no effect ( P ≤ 0.33) of corn processing on plasma glucose, plasma GIP concentrations, hot carcass weight, dressing percentage, or marbling score. There was a positive linear relationship ( P = 0.03) between IM fat concentration and plasma GIP concentration. In conclusion, feeding CC increased gain:feed ratio compared with WSC, but there was no difference in plasma GIP concentration, whereas plasma GIP concentration appears to be related to IM fat deposition., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science.)

The objectives of this study were to evaluate the effect of the use of yeast fermentation products (YFP) on growth, hormone concentration, and gut permeability in dairy calves. One hundred and twenty heifers were randomly assigned to one of three treatments: control group with no YFP supplementation (C), Saccharomyces cerevisiae fermentation products (SCFP) supplementation (1 g/head/d of SmartCare [Diamond V] in the milk and 0.7% on dry matter basis of NutriTek [Diamond V] on the starter feed), or Aspergillus oryzae fermentation extracts (AOFE) supplementation (3 g/head/d of LXtract1224 [Biozyme Inc.] in the milk). All calves received 6 L/d of pasteurized milk and had ad libitum access to water and dry feed along the study. Body weight (BW) was recorded at birth and on days 14, 30, and 45 and at weaning. Dry feed (starter) offered was measured daily and refusals twice a week to obtain starter intake (SI). Diarrhea events were recorded daily and fecal scores were classified by using a four-point scale. Blood was sampled on days 7 and 14 for plasma glucose, nonesterified fatty acids (NEFA), insulin, and IL-1β concentrations. Lactulose and D-mannitol were included in the morning feeding of day 14 and blood samples were taken an hour after feeding for assessment of intestinal permeability. On day 14, blood samples were taken for plasma glucagon-like peptide 2 (GLP-2) concentration. On day 30, fecal samples were collected for measurements of Salmonella and Escherichia coli concentration on feces. No treatment differences ( P ≥ 0.13) were found for BW or SI. There was a time by treatment difference ( P = 0.01) in average daily gain (ADG) on day 45 where C animals had a greater ADG when compared with SCFP and AOFE. Diarrhea incidence did not change between treatments ( P = 0.97) and Salmonella and E. coli were not found in feces. There were no differences ( P > 0.60) between treatments for plasma GLP-2, glucose, insulin, lactulose, nor D-mannitol concentrations. There was a time by treatment tendency ( P = 0.06) for NEFA concentration which tended to be greater on day 7 for C and AOFE when compared with day 14. Plasma IL-1β concentration showed a treatment tendency which tended ( P = 0.06) to be greater for SCFP when compared with C. Under the current conditions, supplementation with YFP did not improve performance parameters. Plasma GLP-2 concentration, intestinal permeability, and plasma metabolites did not differ after yeast fermentation products supplementation., (© The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Changes in the physiological, psychological, and behavioral manifestations of stress have been observed in association with increases in circulating oxytocin (OXT). Providing OXT intranasally has been shown to attenuate stressor-induced hypothalamo-pituitary-adrenal (HPA) axis activation in humans and rodents; however, anxiolytic effects may be context and species specific. The present study aimed to investigate the effect of intranasal OXT supplementation on stressor-induced activation of the HPA axis in beef cattle. We hypothesized that OXT would attenuate activation of the HPA axis, ultimately decreasing plasma cortisol and adrenocorticotropic hormone (ACTH). Twenty-eight Bos taurus heifers were blocked by bodyweight and randomly allocated to one of four treatment groups, in a 2 × 2 factorial arrangement: (1) saline, isolated, standing, and unrestrained (S-isolation stress [IS], 0.015 mL/kg BW 0.9% isotonic saline, n = 7); (2) saline, isolated, and restrained (S-restraint and isolation stress [RIS]; 0.015 mL/kg BW 0.9% isotonic saline; n = 7); (3) OXT, IS (OXT-IS, 0.3 IU/kg BW oxytocin; n = 7); and (4) OXT and RIS (OXT-RIS, 0.3 IU/kg BW oxytocin; n = 7). Oxytocin and saline were administered intranasally. Intranasal treatments were given followed by a waiting time of 30 min when each of the stress treatments was applied for 2 h. Blood samples were collected via jugular catheters directly after stressor application and every 10 min thereafter, for 2 h. Cortisol concentrations increased over time in animals exposed to RIS (P < 0.01) and decreased over time in animals exposed to IS (P < 0.01). Concentrations of ACTH decreased over time for the IS-treated heifers but remained elevated for the RIS-treated heifers (P < 0.01). Under the conditions of the present study, OXT treatment did not affect measured indicators of HPA axis activation. A treatment × time interaction (P < 0.01) was detected for OXT, such that OXT heifers exhibited greater initial OXT concentrations followed by a decline; saline-treated heifers had consistently stable oxytocin concentrations. The RIS-treated heifers increased their glucose (P < 0.01) and lactate (P < 0.01) concentrations throughout the application of the stressors compared with the IS-treated heifers. Overall, restraint stress increased cortisol and oxytocin in B taurus heifers compared with heifers subjected only to isolation. Finding a more intermediate stress model may better allow for detection of the effects of oxytocin on the stress response., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Cows transitioning from late gestation to early lactation experience an increase in energy demands, which lead to a negative energy balance (NEB) because the greater energy requirement is not fully synchronized with the intake of dry matter. In this context, there is an increase in plasma NEFA and ghrelin concentrations and a decrease in plasma insulin and glucose-dependent insulinotropic polypeptide (GIP) concentrations. This situation could have a negative impact on the return to cyclicity because some of these variables have been associated with reduced GnRH and LH pulsatility (high NEFA and low insulin concentrations). However, there are no studies showing the relationship between ghrelin or GIP and reproductive performance. It is known that these hormones are related with lipolysis and NEB, with NEB being one of the main determinants of GnRH pulse generator activity. Thus, the objective of the present study was to evaluate the association between plasma NEFA concentration and metabolic hormones (insulin, ghrelin, and GIP) before parturition and their associations with the resumption of postpartum ovulations in dairy cows. A completely randomized block design was used in a commercial dairy herd with sampling day (visit to farm) as the blocking criteria. Holstein cows (n = 92) were screened for plasma NEFA concentration -5 d (±2 d) relative to the expected parturition day, and top and bottom quartiles were considered as high (H-NEFA) and low (L-NEFA) NEFA groups. Data were analyzed with correlation, linear regression, and proportional hazard regression models. Plasma NEFA concentration (H-NEFA mean = 294 μM, SD = 141.2; and L-NEFA mean = 122 μM, SD = 25.3) was correlated (P < 0.01) with plasma insulin (r = -0.374) and ghrelin (r = -0.346) concentrations but not with plasma GIP concentration (P = 0.64). The greater the concentration of insulin, the lesser the prepartum NEFA concentration (for each 1 μU/mL of plasma insulin increase, there is a decrease of 1.223 ± 0.62 μM of NEFA). Plasma ghrelin and GIP concentrations were not associated with plasma NEFA concentration. Finally, H-NEFA prepartum cows were less likely to resume ovulation than L-NEFA cows (hazard ratio [HR] = 0.563, 95% confidence interval [CI] = 0.314-1.011), whereas high ghrelin cows were more likely to resume ovulation than low ghrelin cows (HR = 1.873, 95% CI = 0.846-4.145). Conversely, resumption of ovulation was not associated with prepartum insulin and GIP concentrations. Prepartum NEFA and possibly ghrelin are associated with the return to postpartum cyclicity; however, insulin and GIP are not related to the resumption of ovulation in dairy cows., (Copyright © 2019 Elsevier Inc. All rights reserved.)