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A recent study showed that just one point mutation F33 to Y in the complementarity-determining region 1 of heavy chain (H-CDR1) could lead to the auto-antibody losing its DNA binding ability. However, the potential molecular mechanisms have not been well elucidated. In this study, we investigated how the antibody lost the DNA binding ability caused by mutation F33 to Y in the H-CDR1. We found that the electrostatic force was not the primary driving force for the interaction between anti-DNA antibodies and the antigen single strand DNA (ssDNA), and that the H-CDR2 largely contributed to the binding of antigen ssDNA, even larger than H-CDR1. The H-F33Y mutation could increase the hydrogen-bond interaction but impair the pi-pi stacking interaction between the antibody and ssDNA. We further found that F33H, W98H and Y95L in the wiletype antibody could form the stable pi-pi stacking interaction with the nucleotide bases of ssDNA. However, the Y33 in mutant could not form the parallel sandwich pi-pi stacking interaction with the ssDNA. To further confirm the importance of pi-pi stacking, the wildtype antibody and the mutants (F33YH, F33AH, W98AH and Y95AL) were experimentally expressed in CHO cells and purified, and the results from ELISA clearly showed that all the mutants lost the ssDNA binding ability. Taken together, our findings may not only deepen the understanding of the underlying interaction mechanism between autoantibody and antigen, but also broad implications in the field of antibody engineer. [ABSTRACT FROM AUTHOR]

Passive acoustic monitoring (PAM) is an effective remote sensing approach for sampling acoustically active animal species and is particularly useful for elusive, visually cryptic species inhabiting remote or inaccessible habitats. Key advantages of PAM are large spatial coverage and continuous, long‐term monitoring. In most cases, a signal detection algorithm is utilized to locate sounds of interest within long sequences of audio data. It is important to understand the demographic/contextual usage of call types when choosing a particular signal to use for detection. Sampling biases may result if sampling is restricted to subsets of the population, for example, when detectable vocalizations are produced only by a certain demographic class. Using the African forest elephant repertoire as a case study, we test for differences in call type usage among different age‐sex classes. We identified disproportionate usage by age‐sex class of four call types—roars, trumpets, rumbles, and combination calls. This differential usage of signals by demographic class has implications for the use of particular call types in PAM for this species. Our results highlight that forest elephant PAM studies that have used rumbles as target signals may have under‐sampled adult males. The addition of other call types to PAM frameworks may be useful to leverage additional population demographic information from these surveys. Our research exemplifies how an examination of a species' acoustic behavior can be used to better contextualize the data and results from PAM and to strengthen the resulting inference. [ABSTRACT FROM AUTHOR]

Background: The Atlantic cod is a prolific species in the Atlantic, despite its inconsistent specific antibody response. It presents a peculiar case within vertebrate immunology due to its distinct immune system, characterized by the absence of MHCII antigen presentation pathway, required for T cell-dependent antibody responses. Thorough characterisation of immunoglobulin loci and analysis of the antibody repertoire is necessary to further our understanding of the Atlantic cod's immune response on a molecular level. Results: A comprehensive search of the cod genome (gadmor3.0) identified the complete set of IgH genes organized into three sequential translocons on chromosome 2, while IgL genes were located on chromosomes 2 and 5. The Atlantic cod displayed a moderate germline V gene diversity, comprising four V gene families for both IgH and IgL, each with distinct chromosomal locations and organizational structures. 5'RACE sequencing revealed a diverse range of heavy chain CDR3 sequences and relatively limited CDR3 diversity in light chains. The analysis highlighted a differential impact of V-gene germline CDR3 length on receptor CDR3 length between heavy and light chains, underlining different recombination processes. Conclusions: This study reveals that the Atlantic cod, despite its inconsistent antibody response, maintains a level of immunoglobulin diversity comparable to other fish species. The findings suggest that the extensive recent duplications of kappa light chain genes do not result in increased repertoire diversity. This research provides a comprehensive view of the Atlantic cod's immunoglobulin gene organization and repertoire, necessary for future studies of antibody responses at the molecular level. [ABSTRACT FROM AUTHOR]

Abstract Passive acoustic monitoring (PAM) is an effective remote sensing approach for sampling acoustically active animal species and is particularly useful for elusive, visually cryptic species inhabiting remote or inaccessible habitats. Key advantages of PAM are large spatial coverage and continuous, long‐term monitoring. In most cases, a signal detection algorithm is utilized to locate sounds of interest within long sequences of audio data. It is important to understand the demographic/contextual usage of call types when choosing a particular signal to use for detection. Sampling biases may result if sampling is restricted to subsets of the population, for example, when detectable vocalizations are produced only by a certain demographic class. Using the African forest elephant repertoire as a case study, we test for differences in call type usage among different age‐sex classes. We identified disproportionate usage by age‐sex class of four call types—roars, trumpets, rumbles, and combination calls. This differential usage of signals by demographic class has implications for the use of particular call types in PAM for this species. Our results highlight that forest elephant PAM studies that have used rumbles as target signals may have under‐sampled adult males. The addition of other call types to PAM frameworks may be useful to leverage additional population demographic information from these surveys. Our research exemplifies how an examination of a species' acoustic behavior can be used to better contextualize the data and results from PAM and to strengthen the resulting inference.

The article is devoted to the analysis of the activities of the ballet troupe of M.Jalil Tatar Academic State Opera and Ballet Theater in the Soviet and post-Soviet periods. It shows that after the premiere of the first ballet “Vain Precaution” by P.Hertel in 1939, the troupe continued to master the performances of the classical heritage “Giselle” by A.Adam (1945) and “Swan Lake” by P.Tchaikovsky (1947). At the same time, the troupe was working on creating a national ballet performance. Turning to the historical past and folklore, the authors of the libretto and choreographers immersed the viewer in the life and culture of the Tatar people, and the composers built the score based on centuries-old musical traditions. All this was expressed in the ballets “Shurale” by F.Yarullin (1945) and “Zyugra” by N.Zhiganov (1946). At this time, new productions appear in the repertoire, reflecting the current ideas of Soviet art at the time of creation. These are “Bakhchisarai Fountain” by B.Asafiev (1946) and “Laurencia” by A.Crane (1953). In the 2000s, a number of premieres took place produced for the Kazan ballet stage – “The Tale of Yusuf” (2001) by L.Lyubovskiy, choreography by N.Boyarchikov, G.Kovtun; “Peer Gynt” (2003) by E.Grieg, “The Golden Horde” (2013), R.Akhiyarova – choreography by G.Kovtun; “Сarmina Вurana, or the Wheel of Fortune” (2013) by K.Orff, choreography by A.Polubentsev. The author sets the task of identifying trends in the development of the repertoire in the post-Soviet period. His focus is on original ballet performances. The conclusion was that the result of the search for choreographers on the Kazan stage, expressed in the synthesis of genres, styles, and appeal to new subjects for the ballet theater, was the enrichment of the repertoire of M.Jalil Tatar Academic State Opera and Ballet Theater and entertainment culture in general.

Abstract Background The Atlantic cod is a prolific species in the Atlantic, despite its inconsistent specific antibody response. It presents a peculiar case within vertebrate immunology due to its distinct immune system, characterized by the absence of MHCII antigen presentation pathway, required for T cell-dependent antibody responses. Thorough characterisation of immunoglobulin loci and analysis of the antibody repertoire is necessary to further our understanding of the Atlantic cod’s immune response on a molecular level. Results A comprehensive search of the cod genome (gadmor3.0) identified the complete set of IgH genes organized into three sequential translocons on chromosome 2, while IgL genes were located on chromosomes 2 and 5. The Atlantic cod displayed a moderate germline V gene diversity, comprising four V gene families for both IgH and IgL, each with distinct chromosomal locations and organizational structures. 5’RACE sequencing revealed a diverse range of heavy chain CDR3 sequences and relatively limited CDR3 diversity in light chains. The analysis highlighted a differential impact of V-gene germline CDR3 length on receptor CDR3 length between heavy and light chains, underlining different recombination processes. Conclusions This study reveals that the Atlantic cod, despite its inconsistent antibody response, maintains a level of immunoglobulin diversity comparable to other fish species. The findings suggest that the extensive recent duplications of kappa light chain genes do not result in increased repertoire diversity. This research provides a comprehensive view of the Atlantic cod's immunoglobulin gene organization and repertoire, necessary for future studies of antibody responses at the molecular level.

Copyright of KADEM Journal of Women's Studies is the property of Women & Justice Association / Kadin & Demokrasi Dernegi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Polymorphisms in the KIR and HLA genes contribute to the diversity of the NK cell repertoire. Extrinsic factors also play a role in modifying this repertoire. The best example is cytomegalovirus, which promotes the expansion of memory-like NK cells. However, the mechanisms governing this phenotypic structure are poorly understood. Furthermore, the influence of age and sex has been understudied. Methods: In this study, we examined these parameters in a cohort of 200 healthy volunteer blood donors, focusing on the major inhibitory KIR receptors and CD94/NKG2A, as well as the differentiation marker CD57 and the memory-like population marker NKG2C. Flow cytometry and two joint analyses, unsupervised and semi-supervised, helped define the impact of various intrinsic and extrinsic markers on the phenotypic structure of the NK cell repertoire. Results: In the KIR NK cell compartment, the KIR3DL1 gene is crucial, as unexpressed alleles lead to a repertoire dominated by KIR2D interacting only with HLA-C ligands, whereas an expressed KIR3DL1 gene allows for a greater diversity of NK cell subpopulations interacting with all HLA class I ligands. KIR2DL2 subsequently favors the KIR2D NK cell repertoire specific to C1/C2 ligands, whereas its absence promotes the expression of KIR2DL1 specific to the C2 ligand. The C2C2Bw4+ environment, marked by strong -21T motifs, favors the expansion of the NK cell population expressing only CD57, whereas the absence of HLA-A3/A11 ligands favors the population expressing only NKG2A, a population highly represented within the repertoire. The AA KIR genotype favors NK cell populations without KIR and NKG2A receptors, whereas the KIR B+ genotypes favor populations expressing KIR and NKG2A. Interestingly, we showed that women have a repertoire enriched in CD57- NK cell populations, while men have more CD57+ NK cell subpopulations. Discussion: Overall, our data demonstrate that the phenotypic structure of the NK cell repertoire follows well-defined genetic rules and that immunological history, sex, and age contribute to shaping this NK cell diversity. These elements can contribute to the better selection of hematopoietic stem cell donors and the definition of allogeneic NK cells for cell engineering in NK cell-based immunotherapy approaches.cters are displayed correctly. [ABSTRACT FROM AUTHOR]

The scientific research is devoted to the disclosure of the most characteristic features and distinctive particularities of the All-Ukrainian festival competition of academic wind music "Magic Flute". This festival-competition has been held by teachers of the academic wind music and percussion department of the Kryvyi Rih Regional Professional Music College for fifty years. The "Magic Flute" is implemented with the constant support of the educational institution's administration and headed by the director of Musical College, L.V. Rakityanska. On the half-century anniversary, the specialized festival competition showed the uniqueness of its creative face. There are maximizing the introduction of various musical and performance forms (solo, ensemble, and orchestral performance on wind and percussion instruments), constant diversification of the repertoire palette (academic masterpieces, works of various cultural and historical periods), involvement of the broadest possible professional wind and percussion academic instruments, as well as artistic and patriotic relaying of the annual holding of this professional and specialized forum of academic wind musicians and percussionists of the Kryvyi Rih region are creating the achievements in the fiftieth anniversary of the artistic life for the "Magic Flute" academic wind festival-competition. The method of historical and comparative analysis of the most significant events in the chronicle of the "Magic Flute" festival-competition, we will emphasize, with the subsequent determination of the stages of formation and development of this large-scale festival-competition event can become a significant prospect for further scientific investigations regarding the specified topic of the presented scientific article. [ABSTRACT FROM AUTHOR]

ABSTRACT: Pekin ducks are exposed to stressors such as heat stress, enteric pathogens, mycotoxins, and other environmental stressors. We know from wild bird literature that birds communicate through vocalizations. We hypothesized that Pekin ducks would have a diverse repertoire that is affected by the sex, social group, and specific stimuli. We utilized adult Pekin ducks to develop a vocal repertoire. We placed 1 to 4 ducks of varying sexes into a sound chamber with various stimuli used to encourage new vocalizations. Birds were recorded for 20 min with several variations of number and sexes of ducks. Once the ducks were recorded each vocalization that was clipped was named based on a predetermined naming system. We characterized the vocal system of the ducks under each stimulus and social treatment in 4 ways: overall call rates, call diversity, call repertoire, and call spectral properties. In all cases, normality of residuals and homogeneity of variances for GLM and ANOVA models were confirmed using Proc Univariate (SAS v9.4) where a p ≤ 0.05 was considered significant. We found that Pekin ducks produce up to 16 different vocalizations. The treatments had a significant effect on the overall rate of calls given by the ducks (ANOVA: F6,31 = 8.55, p < 0.0001). Ducks produced the most calls by far when someone was sitting in the chamber with them (30.04 ± 4.45 calls/min). For call diversity, we found that there was a significant main effect of hen number (F218 = 12.21, p = 0.0004) but no main effect of drake number (F3,18 = 3.04, p = 0.0555). Cluster analyses indicated that certain types of calls were given under specific conditions. There were generally 6 major clusters of vocal repertoires (R-square = 0.899, Cubic Clustering Criterion = 9.30). Our results suggest that Pekin ducks are affected by the types of stimuli and social environment in how much they vocalize and in the properties of the calls they use. In addition, males and females differ somewhat in the repertoire of the calls they use, and in the spectral properties of their calls.

The naïve human antibody repertoire has theoretical access to an estimated > 1015 antibodies. Identifying subsets of this prohibitively large space where therapeutically relevant antibodies may be found is useful for development of these agents. It was previously demonstrated that, despite the immense sequence space, different individuals can produce the same antibodies. It was also shown that therapeutic antibodies, which typically follow seemingly unnatural development processes, can arise independently naturally. To check for biases in how the sequence space is explored, we data mined public repositories to identify 220 bioprojects with a combined seven billion reads. Of these, we created a subset of human bioprojects that we make available as the AbNGS database (https://naturalantibody.com/ngs/). AbNGS contains 135 bioprojects with four billion productive human heavy variable region sequences and 385 million unique complementarity-determining region (CDR)-H3s. We find that 270,000 (0.07% of 385 million) unique CDR-H3s are highly public in that they occur in at least five of 135 bioprojects. Of 700 unique therapeutic CDR-H3, a total of 6% has direct matches in the small set of 270,000. This observation extends to a match between CDR-H3 and V-gene call as well. Thus, the subspace of shared (‘public’) CDR-H3s shows utility for serving as a starting point for therapeutic antibody design.

Copyright of Central Asian Journal of Art Studies is the property of Central Asian Journal of Art Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The collapse of the Fundão dam of the Samarco, Vale, and BPH Billiton mining companies in 2015 resulted in the spillage of unprecedented tailings in the Rio Doce Basin, Brazil. It was a disaster of multiple dimensions, generating conflicts between the affected community, mining companies, the government, and the justice system in the search for solutions to recover and repair the damage. Meanwhile, a governance system was created to conduct disaster responses and conflict resolution between those actors. In this article, we investigate how skilled actors in the affected field act to influence disaster governance by producing consequences for policy decisions. The analysis highlights the role of coalitions and action repertories in cooperative and conflicting relationships in the affected people's field. Through mixed-method research, four years of the socio-environmental disaster (2015–2019) were examined through interviews with activists, documentary research, and analysis of protest events. The main argument is that the mobilization of non-state actors in the affected field has influenced disaster governance and the content of compensation policies. Mobilization combined social skills and action repertoires and formed political coalitions to influence decision-making processes. The article contributes to expanding the understanding of the dynamics of political disputes among non-state actors, proposing to broaden the understanding of the repertoires of non-state actors in coalitions to generate consequences for political decisions. [ABSTRACT FROM AUTHOR]

AbstractPast research in the housing-movement literature has assumed a close link between the post-2008 cycle of housing financialization and the resurgence of housing contention in many Western democracies. However, the new cycle of capital accumulation fails to explain why housing movements privilege some strategic forms of contention over others, how tactical innovations occur, and what pathways they develop after cycles of protest. This article returns to the repertoire perspective to analyse the expansion of housing protest repertoires to tenants’ activism in Barcelona. It argues that the repertoire of housing contention has mediated relations between the new cycle of housing financialization and contentious interactions between tenants and landlords in Barcelona’s private-rental sector. Employing the case of the Sindicat de Llogateres (Tenants’ Union of Barcelona) and the Stay Put campaign, this article makes a twofold contribution to explain the transmission and transformation of the established repertoire in the wake of the 2008 financial crisis. [ABSTRACT FROM AUTHOR]

Silesia in the early modern era is an area with a historically unprecedented role, not only in relation to the tradition of Protestant churches, but also Catholic one. A particularly important cultural player there was the Society of Jesus, which used a variety of cultural strategies in its mission. The present article is a systematic review of these forms of activity, organized according to the chronological units. These time categories correspond to the music–theoretical narratives, to the method of meditation codified in Spiritual Exercises, and also to Ludwik Bielawski's zonal theory of musical time. It seems that Silesian Jesuits consciously and consistently used the music performances in the religious culture they animated at all levels of these 'time zones'. Recurrence and periodicity supported the established social habitus and regulated rhythms of the community's cycles of various religious activities. A natural pretext for this regulation was the liturgical cycle, including new elements to the inherited tradition. Elements of this cyclicity may be found in all forms the of Jesuit repertoire and music genres. The community's sacred time was measured out with recurrent performances of music repertoire and stage performances, creating together the rhythm of human life. [ABSTRACT FROM AUTHOR]

The liturgical singing of the Romanian Orthodox Church is monodic, of the Byzantine tradition. From the 19th century, under the influence of the Russian Orthodox Church, multiple voices choral singing penetrates the Romanian worship establishments. The beginnings were timid, isolated, from private initiatives and sometimes encountering the hostility of conservative ecclesiastical traditionalism. After 1959, the introduction of choral singing in the United Romanian Principalities was to be facilitated by state legislation. Choral singing would be used predominantly for the office of the Holy Liturgy. In finding a choral style suitable for the orthodox liturgical ceremonial, three main stylistic directions were outlined, among which the classicalromantic influence created a generous and varied repertoire. [ABSTRACT FROM AUTHOR]

The purpose of the work is to determine the prerequisites and evolutionary processes of the development of the instrumental and performing school in China in the 20th - early 21st centuries. Research methods are based on cultural, historical, comparative approaches based on the principles of knowledge of nature and society. The scientific novelty consists in determining the prerequisites and development of the instrumental and performing school in China, as well as comparing the genres and features of their interaction, which contributed to the formation of new forms of music making. Conclusions. Based on the historical and musicological analysis of the evolution of instrumental and performing arts in China in the 20th and early 21st centuries, it is stated that the development of the phenomenon under study is inseparable from a comprehensive artistic process that covers the entire range of existing genres and forms. At the beginning of the 21st century, Chinese musical culture in general, and its instrumental and performing segment in particular, became an integral part of global musical development, influencing the course of evolution of world musical art. The conducted analysis made it possible to state the rapid growth of all aspects of the instrumental and performing school of the People's Republic of China, the powerful strengthening of the material base, the expansion of the field of education and popularisation of music, the improvement of the quality of training of performing personnel both in China and through the training of Chinese musicians abroad. [ABSTRACT FROM AUTHOR]

Copyright of Bylye Gody is the property of Cherkas Global University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

IntroductionPolymorphisms in the KIR and HLA genes contribute to the diversity of the NK cell repertoire. Extrinsic factors also play a role in modifying this repertoire. The best example is cytomegalovirus, which promotes the expansion of memory-like NK cells. However, the mechanisms governing this phenotypic structure are poorly understood. Furthermore, the influence of age and sex has been understudied.MethodsIn this study, we examined these parameters in a cohort of 200 healthy volunteer blood donors, focusing on the major inhibitory KIR receptors and CD94/NKG2A, as well as the differentiation marker CD57 and the memory-like population marker NKG2C. Flow cytometry and two joint analyses, unsupervised and semi-supervised, helped define the impact of various intrinsic and extrinsic markers on the phenotypic structure of the NK cell repertoire. ResultsIn the KIR NK cell compartment, the KIR3DL1 gene is crucial, as unexpressed alleles lead to a repertoire dominated by KIR2D interacting only with HLA-C ligands, whereas an expressed KIR3DL1 gene allows for a greater diversity of NK cell subpopulations interacting with all HLA class I ligands. KIR2DL2 subsequently favors the KIR2D NK cell repertoire specific to C1/C2 ligands, whereas its absence promotes the expression of KIR2DL1 specific to the C2 ligand. The C2C2Bw4+ environment, marked by strong -21T motifs, favors the expansion of the NK cell population expressing only CD57, whereas the absence of HLA-A3/A11 ligands favors the population expressing only NKG2A, a population highly represented within the repertoire. The AA KIR genotype favors NK cell populations without KIR and NKG2A receptors, whereas the KIR B+ genotypes favor populations expressing KIR and NKG2A. Interestingly, we showed that women have a repertoire enriched in CD57- NK cell populations, while men have more CD57+ NK cell subpopulations. DiscussionOverall, our data demonstrate that the phenotypic structure of the NK cell repertoire follows well-defined genetic rules and that immunological history, sex, and age contribute to shaping this NK cell diversity. These elements can contribute to the better selection of hematopoietic stem cell donors and the definition of allogeneic NK cells for cell engineering in NK cell-based immunotherapy approaches.cters are displayed correctly.

The article is devoted to the representation of classical music concert in Hollywood cartoons of the 1930s — 1950s. A concert as a symbol of the academic musical culture was well-known to the American cinema audience. The author of the article studies how the cartoons creators rethought the main elements of a concert ceremony using both the general stereotypes related with the world of classical music and the familiar images of American life of the first half of the 20th century. Special attention is focused on the development of a typical concert repertoire in cartoons and its relation to the category of popular classical music. The article gives a detailed analysis of concert types, features of a concert space, the appearance and behaviour of soloists, orchestra players, and conductors, and the evolution of these elements during the Golden Age of Hollywood cartoons. The conclusion is made that a concert in cartoons becomes an ideal object of parody and carnival play which mirror the conflict between high and low art that exists in the mind of the audience and work as assimilation mechanisms for the artistic experience of the elite culture. The methods of history, art studies, and cultural studies are used in the research.

This article is about the associative process that took place in Villa del Cerro in Montevideo, between 1920s and 1950s, a period of boom and crisis of the meat-packing processing industry that was installed in it, trying to make it relevant. It means, the relationships between workspaces and neighborhood, and the influence of both dimensions in the formation ofworker's identity. The reconstruction of this associative process was carried outfrom secondary sources, local press and a large set of texts based on testimonies, observing that the numerous and diverse organizations that were created in the Cerro nurtured the experiences and repertoires of their neighbors and workers, contributing meaningfully to the formation of their identity. [ABSTRACT FROM AUTHOR]

Wind band has a deep and long-lasting connection to folk-song sources. Societal expectations around music choices and sources are changing, including calls to diversify repertoire and eliminate traditional songs that include offensive language and stereotypes. Concerns about music usage in relation to cultural appropriation are also being amplified. How will teachers, composers, and publishers of band music respond to these calls for change? [ABSTRACT FROM AUTHOR]

Copyright of Central Asian Journal of Art Studies is the property of Central Asian Journal of Art Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Canadian Journal of Law & Society/Revue Canadienne Droit et Societe (Cambridge University Press) is the property of Cambridge University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Many autoimmune diseases are characterized by the activation of autoreactive T cells. The T cell repertoire is established in the thymus; it remains uncertain whether the presence of disease-associated autoreactive T cells reflects abnormal T cell selection in the thymus or aberrant T cell activation in the periphery. Here, we describe T cell selection, activation, and T cell repertoire diversity in female mice deficient for B lymphocyte-induced maturation protein (BLIMP)-1 in dendritic cells (DCs) (Prdm1 CKO). These mice exhibit a lupus-like phenotype with an expanded population of T follicular helper (Tfh) cells having a more diverse T cell receptor (TCR) repertoire than wild-type mice and, in turn, develop a lupus-like pathology. To understand the origin of the aberrant Tfh population, we analyzed the TCR repertoire of thymocytes and naive CD4 T cells from Prdm1 CKO mice. We show that early development and selection of T cells in the thymus are not affected. Importantly, however, we observed increased TCR signal strength and increased proliferation of naive T cells cultured in vitro with antigen and BLIMP1-deficient DCs compared to control DCs. Moreover, there was increased diversity in the TCR repertoire in naive CD4+ T cells stimulated in vitro with BLIMP1-deficient DCs. Collectively, our data indicate that lowering the threshold for peripheral T cell activation without altering thymic selection and naive T cell TCR repertoire leads to an expanded repertoire of antigen-activated T cells and impairs peripheral T cell tolerance. [ABSTRACT FROM AUTHOR]

Invasive fungal diseases are a public health problem. They affect a constantly increasing number of at-risk patients, and their incidence has risen in recent years. These opportunistic infections are mainly due to Candida sp. but less common or rare yeast infections should not be underestimated. These so-called "less common" yeasts include Ascomycota of the genera Candida (excluding the five major Candida species), Magnusiomyces/Saprochaete, Malassezia, and Saccharomyces, and Basidiomycota of the genera Cryptococcus (excluding the Cryptococcus neoformans/gattii complex members), Rhodotorula, and Trichosporon. The aim of this review is to (i) inventory the less common yeasts isolated in humans, (ii) provide details regarding the specific anatomical locations where they have been detected and the clinical characteristics of the resulting infections, and (iii) provide an update on yeast taxonomy. Of the total of 239,890 fungal taxa and their associated synonyms sourced from the MycoBank and NCBI Taxonomy databases, we successfully identified 192 yeasts, including 127 Ascomycota and 65 Basidiomycota. This repertoire allows us to highlight rare yeasts and their tropism for certain anatomical sites and will provide an additional tool for diagnostic management. [ABSTRACT FROM AUTHOR]

Like other literary genres, songs can be a successful outlet for achieving certain goals. Using a qualitative and descriptive approach utilizing the stancetaking framework, this study examines the development and use of more than 50 Arabic songs as a vehicle to not only inform the public about COVID-19, but also take affective stances that connect the current distressing situation of the Arab people due to COVID-19 with past hardships and crises. These stances invoke salient sociocultural values that are understood and evaluated similarly by Arab people. Evaluating and positioning the insecurity of the pandemic against insecurities of the past activates the affects and messages of the stance, which align with the evaluations and affects of the listeners. That Arab people from different countries share similar evaluations of their insecurities and invoke similar sociocultural values in response to their dreadful situations is indicative of the co-construction of a shared Arab identity through the sociocognitive intersubjectivity of the stances that are indexed to shared repertoires, sociocultural values, and affects. [ABSTRACT FROM AUTHOR]

The research objective of this article is to present the way in which the Jewish Theater in Warsaw has been building its image. To achieve this goal, the case study method was used. The article takes a closer look at the history of the theatre itself, its founder, the legendary actress Ida Kamińska, the troubles with the seat, but most importantly it presents the image transformations that the theatre has been implementing systematically during the time it has been managed by Gołda Tencer. The article was based on the analysis of collected data, Internet searches and author’s observations. The research shows that thanks to the gradual introduction of the rebranding process, the Jewish Theater has gained new audiences, strengthened its image and managed to build a strong brand in the theatre sector. [ABSTRACT FROM AUTHOR]

Vocal communication is widespread in animals, with vocal repertoires of varying complexity. The social complexity hypothesis predicts that species may need high vocal complexity to deal with complex social organization (e.g. have a variety of different interindividual relations). We quantified the vocal complexity of two geographically distant captive colonies of rooks, a corvid species with complex social organization and cognitive performances, but understudied vocal abilities. We quantified the diversity and gradation of their repertoire, as well as the inter-individual similarity at the vocal unit level. We found that males produced call units with lower diversity and gradation than females, while song units did not differ between sexes. Surprisingly, while females produced highly similar call repertoires, even between colonies, each individual male produced almost completely different call repertoires from any other individual. These findings question the way male rooks communicate with their social partners. We suggest that each male may actively seek to remain vocally distinct, which could be an asset in their frequently changing social environment. We conclude that inter-individual similarity, an understudied aspect of vocal repertoires, should also be considered as a measure of vocal complexity.

An author's creative process cannot be separated from the storehouse of knowledge obtained from different results of reading, hearing, or observing of the events around him. This store of knowledge, when juxtaposed with the concept introduced by Wolfgang Iser in his book The Act of Reading: A Theory of Aesthetic Response (1987), can be called a Repertoire. Shortly, repertoire can be understood as the basis for creating a work, as the background to make the foreground the author aims at through his work. This process also applies to Akutagawa Ryuunosuke's short story entitled Rashomon as the foreground of Konjakumonogatari, the 29th volume of the 18th story. This research aims to describe how the social, historical, and cultural writings by Akutagawa Ryunosuke in the Rashomon and compared with Konjakumonogatari, using the Aesthetic Repertoire theory proposed by Wolfgang Iser. The process through which, among others, grouping the data to be analyzed is related to social, historical, and cultural norms of Japanese society. Next, compare the data to see the relationship between Rashomon and Konjakumonogatari. The results showed (1) There are similarities between social, historical, and cultural similarities between literature and reality, (2) Social norms indicate the life of the Japanese lower class in the Heian period called Genin, (3) Historical norms show the dark conditions that Japanese people went through in the Heian era, because of the many problems that occurred at that time, and (4) Cultural Norms show the efforts made by Japanese people in the Heian period to survive despite hurting others.

The State Academic Chapel ”Doina” is the oldest and most significant performing choir in the Republic of Moldova. An important stage of development occurred during the Second World War: in 1942, the chapel was reorganized into the Doina Song and Dance Ensemble, which brought together musicians, singers and dancers from the Moldovan State Philharmonic. The author considers issues related to the life of the choir collective during the evacuation period and the implementation of concert activities in military field conditions. The geography of the ensemble’s tours is studied, the selection of the repertoire and the interaction of the administration with composers, poets, and heads of other organizations are analyzed. Particular attention is paid to the organization of everyday life and the life of the artists of the Doina Ensemble in wartime conditions. The author uses all available archival materials from the funds of the National Archives of the Republic of Moldova, memoirs of the artists of the ensemble, reminiscences of eyewitnesses, biographies of the artistic directors of the ensemble.

Drawing on Translanguaging theory, which views language as a holistic repertoire, this study examines how students’ translanguaging practices (TP) are manifested and for what purposes. The participants were seven fifth-grade peers in a bilingual school in Brazil (Brazilian Portuguese - English). Their TP were recorded during an oral task based on a wordless, short video stimulus. The data were qualitatively analyzed and coded. Results indicate that TP occurred spontaneously and for specific purposes. The most frequent TP use included gestures, task management, meaning negotiation, lexical creativity, syntactic translanguaging, peer correction, and form negotiation. Findings suggest that translanguaging pedagogy is valuable in bilingual contexts where minority languages are developed because it enables students to communicate freely and critically in a fluid and dynamic environment.

Incorporating students' home languages into the classroom is one powerful way to welcome and show immigrant students care. But what if teachers can't speak these languages? Sharing findings from a collaborative educational ethnography in a large urban ESL program in the U.S., this article offers a framework that new and practicing teachers can use to create warm relationships, support academic progress, and challenge inequality within their classrooms, even when their own linguistic repertoires overlap little with those of their students. [ABSTRACT FROM AUTHOR]

This article retraces a discussion with Gurba M. L., a Sahrawi activist who participated in building the refugee camp of Smara near Tindouf, Algeria, and Lahsen S. S. B, a Sahrawi researcher and collaborator in the Oral Memory Conservation project of the Ministry of Culture. Drawing on fieldwork and recent scholarship in performance studies and architectural history, it describes the repertoire as an infrastructure for historiographical crafts and alternative to the archives in transmitting and preserving gestures, movements and orality for the Sahrawi people. As performed ephemerally through distinct spatial practices in the domesticities of the camp, the repertoire constructs space and history outside of prevalent judiciary and nation-state paradigms that bias documentary evidence to legitimize claims to space and land. Gurba's performance of nomadic spatial practices illuminates broader Sahrawi resistance efforts seeking to realize epistemic and reparative justice, maintaining the futurities preempted by war and colonialism, while conflict is ongoing, refugees displaced, and Western Sahara's plentiful deserts plundered. [ABSTRACT FROM AUTHOR]

The vocalisations of the Grey Shrikethrush are loud, clear, pleasing to the ear, and a stand-out feature of the bird song in areas in which they occur. However, data on these vocalisations in the literature are sparse, as are any attempts at either quantitative or qualitative analysis. Consequently, almost nothing is known about the character of this very obvious song. We made eight recordings of the dawn song of a male individual at the start of the breeding season on the south-east coast of Australia, in spotted gum forest, 350 km south of Sydney. The song comprised discreet groups of notes which we call syllables. The syllables are sung about every 5-10 seconds, and the individual produced 20 different syllables over the course of the eight recordings. Various analyses revealed that new syllables were added gradually over the recording period, and reached a maximum number at the sixth recording, nine days after the first recording. The frequency of use of particular syllables varied markedly, it was common for some syllables to be used as repeats, particular heterogeneous syllable couplets were commonly used as alternations, and the sequence of syllables could be remarkably similar between different recordings. We suggest a way forward to determine whether these patterns are a common feature of the vocalisations of this species. [ABSTRACT FROM AUTHOR]

Drawing on Translanguaging theory, which views language as a holistic repertoire, this study examines how students' translanguaging practices (TP) are manifested and for what purposes. The participants were seven fifth-grade peers in a bilingual school in Brazil (Brazilian Portuguese - English). Their TP were recorded during an oral task based on a wordless, short video stimulus. The data were qualitatively analyzed and coded. Results indicate that TP occurred spontaneously and for specific purposes. The most frequent TP use included gestures, task management, meaning negotiation, lexical creativity, syntactic translanguaging, peer correction, and form negotiation. Findings suggest that translanguaging pedagogy is valuable in bilingual contexts where minority languages are developed because it enables students to communicate freely and critically in a fluid and dynamic environment. [ABSTRACT FROM AUTHOR]

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T-cell mediated immunity relies on a vast array of antigen specific T cell receptors (TR). Characterizing the structure of TR loci is essential to study the diversity and composition of T cell responses in vertebrate species. The lack of good-quality genome assemblies, and the difficulty to perform a reliably mapping of multiple highly similar TR sequences, have hindered the study of these loci in non-model organisms. High-quality genome assemblies are now available for the two main genera of Salmonids, Salmo and Oncorhynchus. We present here a full description and annotation of the TRB loci located on chromosomes 19 and 25 of rainbow trout (Oncorhynchus mykiss). To get insight about variations of the structure and composition of TRB locus across salmonids, we compared rainbow trout TRB loci with other salmonid species and confirmed that the basic structure of salmonid TRB locus is a double set of two TRBV-D-J-C loci in opposite orientation on two different chromosomes. Our data shed light on the evolution of TRB loci in Salmonids after their whole genome duplication (WGD). We established a coherent nomenclature of salmonid TRB loci based on comprehensive annotation. Our work provides a fundamental basis for monitoring salmonid T cell responses by TRB repertoire sequencing. [ABSTRACT FROM AUTHOR]

Vitiligo is an autoimmune depigmentation dermatosis induced by melanocyte destruction, and CD8+ T cells play a pivotal role in melanocyte destruction. However, an accurate profile of the CD8+ T cell receptor (TCR) repertoire in vitiligo patients has not been reported, and the clonotype features of the involved CD8+ T cells remain largely unknown. This study aimed to assess the TCRβ chain repertoire diversity and composition of blood in nine nonsegmental vitiligo patients via high-throughput sequencing. Vitiligo patients manifested a low TCRβ repertoire diversity with highly expanded clones. Differential usage of TRBV, the TRBJ gene, and the TRBV/TRBJ combination were compared between patients with vitiligo and healthy controls. A set of TRBV/TRBJ combinations could differentiate patients with vitiligo from healthy controls (area under the curve = 0.9383, 95% CI: 0.8167–1.00). Our study revealed distinct TCRβ repertoires of CD8+ T cells in patients with vitiligo and will help explore novel immune biomarkers and potential therapeutic targets for vitiligo. • TCR repertoire diversity in patients with vitiligo is less heterogeneous. • Differential usage of TRBV, the TRBJ gene, and the TRBV/TRBJ combination were in vitiligo. [ABSTRACT FROM AUTHOR]

T cell receptor beta chain (TCRβ) diversity (Dβ) gene segments are highly conserved across evolution, with trout Dβ1 sequence identical to human and mouse Dβ1. A key conserved feature is enrichment for glycine in all three Dβ reading frames (RFs). Previously, we found that replacement of mouse Dβ1 with a typical immunoglobulin DH sequence, which unlike Dβ is enriched for tyrosine, leads to an increase in the use of tyrosine in TCRβ complementarity determining region 3 (CDR-B3) after thymic selection, altering T cell numbers, CDR-B3 diversity, and T cell function. To test whether the incorporation of charged amino acids into the Dβ sequence in place of glycine would also influence T cell biology, we targeted the TCRβ locus with a novel glycine-deficient DβDKRQ allele that replaces Dβ1 coding sequence with charged amino acids in all three reading frames. Developing T cells using DβDKRQ expressed TCR CDR-B3s depleted of tyrosine and glycine and enriched for germline-encoded lysine, arginine, and glutamine. Total thymocytes declined in number during the process of β selection that occurs during the transition from the DN3bc to DN4 stage. Conventional thymocyte and T cell numbers remained reduced at all subsequent thymic stages and in the spleen. By contrast, regulatory T cell numbers were increased in Peyer's patches and the large intestine. In terms of functional consequences, T cell reactivity to an ovalbumin immunodominant epitope was reduced. These findings buttress the view that natural selection of Dβ sequence is used to shape the pre-immune TCRβ repertoire, affecting both conventional and regulatory T cell development and influencing epitope recognition. [ABSTRACT FROM AUTHOR]

This paper examines the manifestation of individual style through the lens of a specific language category: the interjection. The analysis considers how interjections are used as a resource in the dramatic dialogue of three Restoration playwrights: Aphra Behn, John Dryden and Thomas D'Urfey, and how their preferences and practices of use compare to previously identified trends in the history of English. Using the concept of the repertoire as a frame for situated language use, the paper examines how genre, time, and characterisation shape the selection and frequency of interjections in the plays of each author. Corpus linguistic methods are used to provide a quantitative and qualitative overview of each author's interjection repertoire. The results suggest that whilst genre, time, and characterisation are influential in shaping the selection and implementation of interjection forms, the choice of expressive language in dramatic contexts is also distinctive and coherent at an authorial level. [ABSTRACT FROM AUTHOR]

While studies have shown benefits of plurilingual pedagogies on students' experiences learning languages, more research is needed to examine how these pedagogies can be enacted in foreign language programmes in digital environments. Moreover, prioritising oral engagement has been an urgent need among teachers who use synchronous platforms such as Zoom to teach languages. This article reports on a multiple case study with three teachers – English, Spanish, and French – and 17 students in Brazil. Five plurilingual strategies were implemented in their language courses: cross-linguistic comparisons, cross-cultural comparisons, translanguaging, translation for mediation, and pluriliteracies. Inductive analysis of weekly classroom observations (N = 15) and deductive analysis of individual teacher interviews were conducted to find similarities across the three language courses. Results show that digital plurilingual pedagogy mobilised students' entire repertoire (not L1 only), encouraged them to speak in the target language, awakened nonlinguistic semiotic resources, and enhanced plurilingual and pluricultural awareness beyond geographical boundaries. Given its multimodal nature, digital plurilingual pedagogy can facilitate oral engagement differently compared to face-to-face instruction. [ABSTRACT FROM AUTHOR]

This article examines the changes in casting of the role of Komponist in Richard Strauss' Ariadne auf Naxos. The aim of the article is to establish the timeline when this originally soprano role became a staple of the mezzo-soprano category. The research involves an examination of the historical archives of international opera houses such as the Metropolitan Opera in New York, the Vienna State Opera, the Teatro alla Scala in Milan, and the Royal Opera House in London. The conclusion reveals that the shift in voice type took place gradually between the 1960s and 1970s. The conclusion also proposes that the reason for this change lies in the development of the mezzo-soprano's subcategories, which coincides with this period in the late 20th century. [ABSTRACT FROM AUTHOR]

The purpose of the article is to identify the leading trends in the activity of one of the oldest professional creative teams - the H. Maiboroda National Honoured Bandura Choir of Ukraine in the context of the development of academic folk vocal and instrumental art. The research methodology is determined by general philosophical, dialectical and historical principles, systematic and comparative approaches, as well as special musicological methods for the study of bandura instruments, concert and touring activities, repertoire formation trends, and personological aspects of musical creativity. The scientific novelty of the article is a systematic analysis of the creative activity of the H. Maiboroda National Honoured Bandura Choir through the prism of academicisation of folk vocal and instrumental art, development of ensemble bandura performance, selection and optimisation of bandura instruments, formation of repertoire principles, contribution of leading directors and conductors of the band to its professional formation, cooperation with composers and soloists, and the thematic principle in organising concert and touring programmes. Conclusions. The H. Maiboroda National Honoured Bandura Choir of Ukraine is a unique collective with a rich history, accumulation and synthesis of both folk music traditions - kobzarism and academic choral traditions. More than a century of the choir's functioning has become an expression of the cultural peculiarities of the time periods of bandura creativity and performance: at the level of improving the instrumentation, involving other folk and academic instruments in banduras, selecting male voices, choosing the texture of choral singing and instrumental playing, genre and thematic directions of the repertoire, collaborating with soloists and conductors, and a wide geography of concert and touring activities. The priority directions of the repertoire of the choir are folklore samples in a cappella and vocalinstrumental representation and composer's elaboration, original works and arrangements of Ukrainian and foreign composers, covering historical, Cossack, lyrical, humorous, riflemen's songs, Shevchenko's songs, UPA songs, and modern patriotic works. [ABSTRACT FROM AUTHOR]