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Your search keyword '"Reschke, Melanie"' showing total 15 results
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15 results on '"Reschke, Melanie"'

2. Lysis of cold-storage-induced microvascular obstructions for ex vivo revitalization of marginal human kidneys

Author

DiRito, Jenna R., Hosgood, Sarah A., Reschke, Melanie, Albert, Claire, Bracaglia, Laura G., Ferdinand, John R., Stewart, Benjamin J., Edwards, Christopher M., Vaish, Anand G., Thiru, Sathia, Mulligan, David C., Haakinson, Danielle J., Clatworthy, Menna R., Saltzman, W. Mark, Pober, Jordan S., Nicholson, Michael L., and Tietjen, Gregory T.

Published
2021
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3. Polymer nanoparticles deliver mRNA to the lung for mucosal vaccination

Author

Suberi, Alexandra, primary, Grun, Molly K., additional, Mao, Tianyang, additional, Israelow, Benjamin, additional, Reschke, Melanie, additional, Grundler, Julian, additional, Akhtar, Laiba, additional, Lee, Teresa, additional, Shin, Kwangsoo, additional, Piotrowski-Daspit, Alexandra S., additional, Homer, Robert J., additional, Iwasaki, Akiko, additional, Suh, Hee-Won, additional, and Saltzman, W. Mark, additional

Published
2023
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7. Cross-platform analysis reveals cellular and molecular landscape of glioblastoma invasion

Author

Chen, Ann T, primary, Xiao, Yang, additional, Tang, Xiangjun, additional, Baqri, Mehdi, additional, Gao, Xingchun, additional, Reschke, Melanie, additional, Sheu, Wendy C, additional, Long, Gretchen, additional, Zhou, Yu, additional, Deng, Gang, additional, Zhang, Shenqi, additional, Deng, Yanxiang, additional, Bai, Zhiliang, additional, Kim, Dongjoo, additional, Huttner, Anita, additional, Kunes, Russell, additional, Günel, Murat, additional, Moliterno, Jennifer, additional, Saltzman, W Mark, additional, Fan, Rong, additional, and Zhou, Jiangbing, additional

Published
2022
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9. Inhalable polymer nanoparticles for versatile mRNA delivery and mucosal vaccination

Author

Suberi, Alexandra, primary, Grun, Molly K., additional, Mao, Tianyang, additional, Israelow, Benjamin, additional, Reschke, Melanie, additional, Grundler, Julian, additional, Akhtar, Laiba, additional, Lee, Teresa, additional, Shin, Kwangsoo, additional, Piotrowski-Daspit, Alexandra S., additional, Homer, Robert J., additional, Iwasaki, Akiko, additional, Suh, Hee Won, additional, and Saltzman, W. Mark, additional

Published
2022
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11. A digital pathology tool for quantification of color features in histologic specimens

Author

Reschke, Melanie, primary, DiRito, Jenna R., additional, Stern, David, additional, Day, Wesley, additional, Plebanek, Natalie, additional, Harris, Matthew, additional, Hosgood, Sarah A., additional, Nicholson, Michael L., additional, Haakinson, Danielle J., additional, Zhang, Xuchen, additional, Mehal, Wajahat Z., additional, Ouyang, Xinshou, additional, Pober, Jordan S., additional, Saltzman, W. Mark, additional, and Tietjen, Gregory T., additional

Published
2021
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12. A digital pathology tool for quantification of color features in histologic specimens.

Author

Reschke, Melanie, DiRito, Jenna R., Stern, David, Day, Wesley, Plebanek, Natalie, Harris, Matthew, Hosgood, Sarah A., Nicholson, Michael L., Haakinson, Danielle J., Zhang, Xuchen, Mehal, Wajahat Z., Ouyang, Xinshou, Pober, Jordan S., Saltzman, W. Mark, and Tietjen, Gregory T.

Subjects
*IMAGE color analysis, *DIGITAL technology, *TISSUE analysis, *COLOR
Abstract

In preclinical research, histological analysis of tissue samples is often limited to qualitative or semiquantitative scoring assessments. The reliability of this analysis can be impaired by the subjectivity of these approaches, even when read by experienced pathologists. Furthermore, the laborious nature of manual image assessments often leads to the analysis being restricted to a relatively small number of images that may not accurately represent the whole sample. Thus, there is a clear need for automated image analysis tools that can provide robust and rapid quantification of histologic samples from paraffin‐embedded or cryopreserved tissues. To address this need, we have developed a color image analysis algorithm (DigiPath) to quantify distinct color features in histologic sections. We demonstrate the utility of this tool across multiple types of tissue samples and pathologic features, and compare results from our program to other quantitative approaches such as color thresholding and hand tracing. We believe this tool will enable more thorough and reliable characterization of histological samples to facilitate better rigor and reproducibility in tissue‐based analyses. [ABSTRACT FROM AUTHOR]

Published
2022
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13. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression

Author

Merola, Jonathan, primary, Reschke, Melanie, additional, Pierce, Richard W., additional, Qin, Lingfeng, additional, Spindler, Susann, additional, Baltazar, Tania, additional, Manes, Thomas D., additional, Lopez-Giraldez, Francesc, additional, Li, Guangxin, additional, Bracaglia, Laura G., additional, Xie, Catherine, additional, Kirkiles-Smith, Nancy, additional, Saltzman, W. Mark, additional, Tietjen, Gregory T., additional, Tellides, George, additional, and Pober, Jordan S., additional

Published
2019
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14. Inhalable polymer nanoparticles for versatile mRNA delivery and mucosal vaccination.

Author

Suberi A, Grun MK, Mao T, Israelow B, Reschke M, Grundler J, Akhtar L, Lee T, Shin K, Piotrowski-Daspit AS, Homer RJ, Iwasaki A, Suh HW, and Saltzman WM

Abstract

An inhalable platform for mRNA therapeutics would enable minimally invasive and lung targeted delivery for a host of pulmonary diseases. Development of lung targeted mRNA therapeutics has been limited by poor transfection efficiency and risk of vehicle-induced pathology. Here we report an inhalable polymer-based vehicle for delivery of therapeutic mRNAs to the lung. We optimized biodegradable poly(amine-co-ester) polyplexes for mRNA delivery using end group modifications and polyethylene glycol. Our polyplexes achieved high transfection of mRNA throughout the lung, particularly in epithelial and antigen-presenting cells. We applied this technology to develop a mucosal vaccine for SARS-CoV-2. Intranasal vaccination with spike protein mRNA polyplexes induced potent cellular and humoral adaptive immunity and protected K18-hACE2 mice from lethal viral challenge., One-Sentence Summary: Inhaled polymer nanoparticles (NPs) achieve high mRNA expression in the lung and induce protective immunity against SARS-CoV-2.

Published
2022
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15. Unadjuvanted intranasal spike vaccine booster elicits robust protective mucosal immunity against sarbecoviruses.

Author

Mao T, Israelow B, Suberi A, Zhou L, Reschke M, Peña-Hernández MA, Dong H, Homer RJ, Saltzman WM, and Iwasaki A

Abstract

As the SARS-CoV-2 pandemic enters its third year, vaccines that not only prevent disease, but also prevent transmission are needed to help reduce global disease burden. Currently approved parenteral vaccines induce robust systemic immunity, but poor immunity at the respiratory mucosa. Here we describe the development of a novel vaccine strategy, Prime and Spike, based on unadjuvanted intranasal spike boosting that leverages existing immunity generated by primary vaccination to elicit mucosal immune memory within the respiratory tract. We show that Prime and Spike induces robust T resident memory cells, B resident memory cells and IgA at the respiratory mucosa, boosts systemic immunity, and completely protects mice with partial immunity from lethal SARS-CoV-2 infection. Using divergent spike proteins, Prime and Spike enables induction of cross-reactive immunity against sarbecoviruses without invoking original antigenic sin., One-Sentence Summary: Broad sarbecovirus protective mucosal immunity is generated by unadjuvanted intranasal spike boost in preclinical model.

Published
2022
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