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24 results on '"Ressmann, Anna K."'

1. Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

Author

Alonso, Sandra, Santiago, Gerard, Cea-Rama, Isabel, Fernandez-Lopez, Laura, Coscolín, Cristina, Modregger, Jan, Ressmann, Anna K., Martínez-Martínez, Mónica, Marrero, Helena, Bargiela, Rafael, Pita, Marcos, Gonzalez-Alfonso, Jose L., Briand, Manon L., Rojo, David, Barbas, Coral, Plou, Francisco J., Golyshin, Peter N., Shahgaldian, Patrick, Sanz-Aparicio, Julia, Guallar, Víctor, and Ferrer, Manuel

Published
2020
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2. Transforming an esterase into an enantioselective catecholase through bioconjugation of a versatile metal-chelating inhibitor

Author

Fernandez-Lopez, Laura, Cea-Rama, Isabel, Alvarez-Malmagro, Julia, Ressmann, Anna K, Gonzalez-Alfinso, Jose Luis, Coscolín, Cristina, Shahgaldian, Patrick, Plou, Francisco J, Modregger, Jan, Pita, Marcos, Sanz-Aparicio, Julia, and Ferrer, Manuel

Subjects
biomimetic catalyst, (+)-catechin, enzymatic metamorphosis, inhibitors
Abstract

Metal complexes introduced into protein scaffolds can generate versatile biomimetic catalysts endowed with a variety of catalytic properties. Here, we synthesized and covalently bound a bipyridinyl derivative to the active centre of an esterase to generate a biomimetic catalyst which shows catecholase activity and enantioselective catalytic oxidation of (+)-catechin.

Published
2023

8. Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes

Author

Schwendenwein, Daniel, Ressmann, Anna K., Entner, Marcello, Savic, Viktor, Winkler, Margit, and Rudroff, Florian

Subjects
Chemistry, Chemical technology, carboxylic acid reductase, Heck-coupling, TP1-1185, ene reductase, QD1-999, chemo-enzymatic cascade, fragrance aldehydes
Abstract

In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and the development of a highly versatile high-throughput assay for the enzymatic reduction of carboxylic acids. We investigated a biocompatible metal-catalyzed synthesis for the preparation of α or β substituted cinnamic acid derivatives which were fed directly into the biocatalytic system. Subsequently, the target molecules were synthesized by an enzymatic cascade consisting of a carboxylate reduction, followed by the selective C-C double bond reduction catalyzed by appropriate enoate reductases. We investigated a biocompatible oxidative Heck protocol and combined it with cells expressing a carboxylic acid reductase from Neurospora crassa (NcCAR) and an ene reductase from Saccharomyces pastorianus for the production fragrance aldehydes.

Published
2021

9. Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

Author

Alonso, Sandra, primary, Santiago, Gerard, additional, Cea-Rama, Isabel, additional, Fernandez-Lopez, Laura, additional, Coscolín, Cristina, additional, Modregger, Jan, additional, Ressmann, Anna K., additional, Martínez-Martínez, Mónica, additional, Marrero, Helena, additional, Bargiela, Rafael, additional, Pita, Marcos, additional, Gonzalez-Alfonso, Jose L., additional, Briand, Manon L., additional, Rojo, David, additional, Barbas, Coral, additional, Plou, Francisco J., additional, Golyshin, Peter N., additional, Shahgaldian, Patrick, additional, Sanz-Aparicio, Julia, additional, Guallar, Víctor, additional, and Ferrer, Manuel, additional

Published
2019
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12. Anti-inflammatory choline based ionic liquids: Insights into their lipophilicity, solubility and toxicity parametrites

Author

Araújo, ANDRÉ, Azevedo, Ana M.O., Costa, Susana P.F., Dias, Ana F.V., Marques, Alexandre H.O., Pinto, Paula C.A.G., Bica, Katharina, Ressmann, Anna K., Passos, Marieta L.C., Reis, Salette, and Saraiva, M. Lúcia M.F.S.

Subjects
Non-steroidal anti-inflammatory drugs Ionic liquids Partition coefficient Shake flask method Cytochrome c oxidase
Abstract

The impact on in vivo efficacy and safety of two novel ionic liquids based on the association of choline with nonsteroidal anti-inflammatory drugs, ketoprofen and naproxen forming IL-APIs, was evaluated. Their lipophilicity, solubility and toxicity were assessed aiming the illustration of the pharmaceutical profile and potential toxic impact. Partition coefficientwas determined usingmicelles of hexadecylphosphocholine and UV–Vis derivative spectroscopy. Additionally, solubility in phosphate buffer pH 7.4 wasmeasured using amodified shake flaskmethod and UV–Vis spectroscopy as detection technique. Ultimately, toxicity was considered resorting to a fully automated cytochrome c oxidase assay based onmicrofluidics. The obtained results demonstrated that the IL-APIs' drug format has the ability to interact with biological membranes and also improves solubility up to 58 times. Moreover, it was evidenced that, although being a nutrient, choline influences the IL-APIs' toxicity. The studied anti-inflammatory IL-APIs exhibited promising properties regarding their incorporation in pharmaceutical formulations.

Published
2017

15. Anti-inflammatory choline based ionic liquids: Insights into their lipophilicity, solubility and toxicity parameters

Author

Azevedo, Ana M.O., primary, Costa, Susana P.F., additional, Dias, Ana F.V., additional, Marques, Alexandre H.O., additional, Pinto, Paula C.A.G., additional, Bica, Katharina, additional, Ressmann, Anna K., additional, Passos, Marieta L.C., additional, Araújo, André R.T.S., additional, Reis, Salette, additional, and Saraiva, M. Lúcia M.F.S., additional

Published
2017
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24. Transforming an esterase into an enantioselective catecholase through bioconjugation of a versatile metal-chelating inhibitor.

Author

Fernandez-Lopez L, Cea-Rama I, Alvarez-Malmagro J, Ressmann AK, Gonzalez-Alfonso JL, Coscolín C, Shahgaldian P, Plou FJ, Modregger J, Pita M, Sanz-Aparicio J, and Ferrer M

Subjects
Stereoisomerism, Oxidation-Reduction, Catalysis, Esterases, Coordination Complexes
Abstract

Metal complexes introduced into protein scaffolds can generate versatile biomimetic catalysts endowed with a variety of catalytic properties. Here, we synthesized and covalently bound a bipyridinyl derivative to the active centre of an esterase to generate a biomimetic catalyst that shows catecholase activity and enantioselective catalytic oxidation of (+)-catechin.

Published
2023
Full Text
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