1. Influence of age on retinochoroidal healing processes after argon photocoagulation in C57bl/6j mice
- Author
-
Dot, C., Parier, V., Behar-Cohen, F., Benezra, D., Jonet, L., Goldenberg, B., Picard, E., Serge Camelo, Kozak, Y., May, F., Soubrane, G., Jeanny, J. C., Picard, Emilie, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Intercommunal de Créteil (CHIC), Hôpital Hôtel-Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assuta Medical Centre-Rishon [Israel], Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Hôpital d'Instruction des Armées Legouest, Service de Santé des Armées, Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP)
- Subjects
Vascular Endothelial Growth Factor A ,Aging ,Aging/pathology ,Animals ,Argon ,Chemokine CCL2/genetics ,Chemokine CCL2/metabolism ,Choroid/blood supply ,Choroid/pathology ,Choroidal Neovascularization/pathology ,Choroidal Neovascularization/surgery ,Gene Expression Regulation ,Glial Fibrillary Acidic Protein/genetics ,Glial Fibrillary Acidic Protein/metabolism ,Laser Coagulation ,Mice ,Mice, Inbred C57BL ,RNA, Messenger/genetics ,RNA, Messenger/metabolism ,Retina/metabolism ,Retina/pathology ,Retinal Pigment Epithelium/pathology ,Retinal Pigment Epithelium/surgery ,Vascular Endothelial Growth Factor A/genetics ,Vascular Endothelial Growth Factor A/metabolism ,Wound Healing ,[SDV]Life Sciences [q-bio] ,Retinal Pigment Epithelium ,Retina ,MESH: Choroid ,MESH: Laser Coagulation ,MESH: Mice, Inbred C57BL ,Glial Fibrillary Acidic Protein ,MESH: Glial Fibrillary Acidic Protein ,MESH: Aging ,MESH: Animals ,RNA, Messenger ,MESH: Mice ,MESH: Chemokine CCL2 ,Chemokine CCL2 ,MESH: RNA, Messenger ,Choroid ,MESH: Argon ,MESH: Choroidal Neovascularization ,MESH: Retina ,MESH: Vascular Endothelial Growth Factor A ,MESH: Gene Expression Regulation ,Choroidal Neovascularization ,MESH: Retinal Pigment Epithelium ,[SDV] Life Sciences [q-bio] ,MESH: Wound Healing ,Research Article - Abstract
International audience; Purpose: To analyze the influence of age on retinochoroidal wound healing processes and on glial growth factor and cytokine mRNA expression profiles observed after argon laser photocoagulation.Methods: A cellular and morphometric study was performed that used 44 C57Bl/6J mice: 4-week-old mice (group I, n=8), 6-week-old mice (group II, n=8), 10-12-week-old mice (group III, n=14), and 1-year-old mice (group IV, n=14). All mice in these groups underwent a standard argon laser photocoagulation (50 microm, 400 mW, 0.05 s). Two separated lesions were created in each retina using a slit lamp delivery system. At 1, 3, 7, 14, 60 days, and 4 months after photocoagulation, mice from each of the four groups were sacrificed by carbon dioxide inhalation. Groups III and IV were also studied at 6, 7, and 8 months after photocoagulation. At each time point the enucleated eyes were either mounted in Tissue Tek (OCT), snap frozen and processed for immunohistochemistry or either flat mounted (left eyes of groups III and IV). To determine, by RT-PCR, the time course of glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and monocyte chemotactic protein-1 (MCP-1) gene expression, we delivered ten laser burns (50 microm, 400 mW, 0.05 s) to each retina in 10-12-week-old mice (group III', n=10) and 1-year-old mice (group IV', n=10). Animals from Groups III' and IV' had the same age than those from Groups III and IV, but they received ten laser impacts in each eye and served for the molecular analysis. Mice from Groups III and IV received only two laser impacts per eye and served for the cellular and morphologic study. Retinal and choroidal tissues from these treated mice were collected at 16 h, and 1, 2, 3, and 7 days after photocoagulation. Two mice of each group did not receive photocoagulation and were used as controls.Results: In the cellular and morphologic study, the resultant retinal pigment epithelium interruption expanse was significantly different between the four groups. It was more concise and smaller in the oldest group IV (112.1 microm+/-11.4 versus 219.1 microm+/-12.2 in group III) p
- Published
- 2020