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153 results on '"Rey, Federica"'

7. Non-Negative Matrix Tri-Factorization for Representation Learning in Multi-Omics Datasets with Applications to Drug Repurposing and Selection.

Author

Messa, Letizia, Testa, Carolina, Carelli, Stephana, Rey, Federica, Jacchetti, Emanuela, Cereda, Cristina, Raimondi, Manuela Teresa, Ceri, Stefano, and Pinoli, Pietro

Subjects
ARTIFICIAL intelligence, DRUG synergism, DRUG repositioning, NONNEGATIVE matrices, DRUG design
Abstract

The vast corpus of heterogeneous biomedical data stored in databases, ontologies, and terminologies presents a unique opportunity for drug design. Integrating and fusing these sources is essential to develop data representations that can be analyzed using artificial intelligence methods to generate novel drug candidates or hypotheses. Here, we propose Non-Negative Matrix Tri-Factorization as an invaluable tool for integrating and fusing data, as well as for representation learning. Additionally, we demonstrate how representations learned by Non-Negative Matrix Tri-Factorization can effectively be utilized by traditional artificial intelligence methods. While this approach is domain-agnostic and applicable to any field with vast amounts of structured and semi-structured data, we apply it specifically to computational pharmacology and drug repurposing. This field is poised to benefit significantly from artificial intelligence, particularly in personalized medicine. We conducted extensive experiments to evaluate the performance of the proposed method, yielding exciting results, particularly compared to traditional methods. Novel drug–target predictions have also been validated in the literature, further confirming their validity. Additionally, we tested our method to predict drug synergism, where constructing a classical matrix dataset is challenging. The method demonstrated great flexibility, suggesting its applicability to a wide range of tasks in drug design and discovery. [ABSTRACT FROM AUTHOR]

Published
2024
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10. 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells

Author

Donnaloja, Francesca, primary, Raimondi, Manuela Teresa, additional, Messa, Letizia, additional, Barzaghini, Bianca, additional, Carnevali, Federica, additional, Colombo, Emanuele, additional, Mazza, Davide, additional, Martinelli, Chiara, additional, Boeri, Lucia, additional, Rey, Federica, additional, Cereda, Cristina, additional, Osellame, Roberto, additional, Cerullo, Giulio, additional, Carelli, Stephana, additional, Soncini, Monica, additional, and Jacchetti, Emanuela, additional

Published
2023
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13. Redox Imbalance in Neurological Disorders in Adults and Children

Author

Rey, Federica, primary, Berardo, Clarissa, additional, Maghraby, Erika, additional, Mauri, Alessia, additional, Messa, Letizia, additional, Esposito, Letizia, additional, Casili, Giovanna, additional, Ottolenghi, Sara, additional, Bonaventura, Eleonora, additional, Cuzzocrea, Salvatore, additional, Zuccotti, Gianvincenzo, additional, Tonduti, Davide, additional, Esposito, Emanuela, additional, Paterniti, Irene, additional, Cereda, Cristina, additional, and Carelli, Stephana, additional

Published
2023
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14. Subcutaneous Adipose Tissue Transcriptome Highlights Specific Expression Profiles in Severe Pediatric Obesity: A Pilot Study

Author

Berardo, Clarissa, primary, Calcaterra, Valeria, additional, Mauri, Alessia, additional, Carelli, Stephana, additional, Messa, Letizia, additional, Destro, Francesca, additional, Rey, Federica, additional, Cordaro, Erika, additional, Pelizzo, Gloria, additional, Zuccotti, Gianvincenzo, additional, and Cereda, Cristina, additional

Published
2023
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15. Oxygen Sensing in Neurodegenerative Diseases: Current Mechanisms, Implication of Transcriptional Response, and Pharmacological Modulation

Author

Rey, Federica, primary, Messa, Letizia, additional, Maghraby, Erika, additional, Casili, Giovanna, additional, Ottolenghi, Sara, additional, Barzaghini, Bianca, additional, Raimondi, Manuela Teresa, additional, Cereda, Cristina, additional, Cuzzocrea, Salvatore, additional, Zuccotti, Gianvincenzo, additional, Esposito, Emanuela, additional, Paterniti, Irene, additional, and Carelli, Stephana, additional

Published
2023
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17. Telomere Length and Mitochondrial DNA Copy Number Variations in Patients with Obesity: Effect of Diet-Induced Weight Loss—A Pilot Study

Author

Cancello, Raffaella, primary, Rey, Federica, additional, Carelli, Stephana, additional, Cattaldo, Stefania, additional, Fontana, Jacopo Maria, additional, Goitre, Ilaria, additional, Ponzo, Valentina, additional, Merlo, Fabio Dario, additional, Zuccotti, Gianvincenzo, additional, Bertoli, Simona, additional, Capodaglio, Paolo, additional, Bo, Simona, additional, and Brunani, Amelia, additional

Published
2022
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21. RNA-seq dataset of subcutaneous adipose tissue: Transcriptional differences between obesity and healthy women

Author

Messa, Letizia, primary, Rey, Federica, additional, Pandini, Cecilia, additional, Barzaghini, Bianca, additional, Micheletto, Giancarlo, additional, Raimondi, Manuela Teresa, additional, Bertoli, Simona, additional, Cereda, Cristina, additional, Zuccotti, Gianvincenzo, additional, Cancello, Raffaella, additional, and Carelli, Stephana, additional

Published
2021
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22. α‐Synuclein antisense transcript SNCA‐AS1 regulates synapses‐ and aging‐related genes suggesting its implication in Parkinson's disease

Author

Rey, Federica, primary, Pandini, Cecilia, additional, Messa, Letizia, additional, Launi, Rossella, additional, Barzaghini, Bianca, additional, Zangaglia, Roberta, additional, Raimondi, Manuela Teresa, additional, Gagliardi, Stella, additional, Cereda, Cristina, additional, Zuccotti, Gian Vincenzo, additional, and Carelli, Stephana, additional

Published
2021
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23. MINCR: A long non-coding RNA shared between cancer and neurodegeneration

Author

Pandini, Cecilia, primary, Garofalo, Maria, additional, Rey, Federica, additional, Garau, Jessica, additional, Zucca, Susanna, additional, Sproviero, Daisy, additional, Bordoni, Matteo, additional, Berzero, Giulia, additional, Davin, Annalisa, additional, Poloni, Tino Emanuele, additional, Pansarasa, Orietta, additional, Carelli, Stephana, additional, Gagliardi, Stella, additional, and Cereda, Cristina, additional

Published
2021
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24. Transcriptional characterization of subcutaneous adipose tissue in obesity affected women highlights metabolic dysfunction and implications for lncRNAs

Author

Rey, Federica, primary, Messa, Letizia, additional, Pandini, Cecilia, additional, Barzaghini, Bianca, additional, Micheletto, Giancarlo, additional, Raimondi, Manuela Teresa, additional, Bertoli, Simona, additional, Cereda, Cristina, additional, Zuccotti, Gian Vincenzo, additional, Cancello, Raffaella, additional, and Carelli, Stephana, additional

Published
2021
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26. Neural Precursor Cells Expanded Inside the 3D Micro-Scaffold Nichoid Present Different Non-Coding RNAs Profiles and Transcript Isoforms Expression: Possible Epigenetic Modulation by 3D Growth

Author

Messa, Letizia, primary, Barzaghini, Bianca, additional, Rey, Federica, additional, Pandini, Cecilia, additional, Zuccotti, Gian Vincenzo, additional, Cereda, Cristina, additional, Carelli, Stephana Carelli, additional, and Raimondi, Manuela Teresa, additional

Published
2021
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28. Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson’s Disease

Author

Rey, Federica, Ottolenghi, Sara, Giallongo, Toniella, Balsari, Alice, Martinelli, Carla, Rey, Robert, Allevi, Raffaele, Giulio, Anna, Zuccotti, Gian, Mazzucchelli, Serena, Foresti, Roberta, Samaja, Michele, Carelli, Stephana, Università degli Studi di Milano = University of Milan (UNIMI), Children's Hospital 'V. Buzzi' [Milan, Italy], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Foresti, Roberta, and University of Milan

Subjects
[SDV] Life Sciences [q-bio], tyrosine hydroxylase (TH), lcsh:Therapeutics. Pharmacology, mitochondrial metabolism, hemic and lymphatic diseases, [SDV]Life Sciences [q-bio], lcsh:RM1-950, Parkinson’s disease, erythropoietin (EPO), neuroprotection, Article, redox imbalance, nervous system diseases
Abstract

Existing therapies for Parkinson&rsquo, s disease (PD) are only symptomatic. As erythropoietin (EPO) is emerging for its benefits in neurodegenerative diseases, here, we test the protective effect driven by EPO in in vitro (SH-SY5Y cells challenged by MPP+) and in vivo (C57BL/6J mice administered with MPTP) PD models. EPO restores cell viability in both protective and restorative layouts, enhancing the dopaminergic recovery. Specifically, EPO rescues the PD-induced damage to mitochondria, as shown by transmission electron microscopy, Mitotracker assay and PINK1 expression. Moreover, EPO promotes a rescue of mitochondrial respiration while markedly enhancing the glycolytic rate, as shown by the augmented extracellular acidification rate, contributing to elevated ATP levels in MPP+-challenged cells. In PD mice, EPO intrastriatal infusion markedly improves the outcome of behavioral tests. This is associated with the rescue of dopaminergic markers and decreased neuroinflammation. This study demonstrates cellular and functional recovery following EPO treatment, likely mediated by the 37 Kda isoform of the EPO-receptor. We report for the first time, that EPO-neuroprotection is exerted through restoring ATP levels by accelerating the glycolytic rate. In conclusion, the redox imbalance and neuroinflammation associated with PD may be successfully treated by EPO.

Published
2021

29. RNA-seq Characterization of Sex-Differences in Adipose Tissue of Obesity Affected Patients: Computational Analysis of Differentially Expressed Coding and Non-Coding RNAs

Author

Rey, Federica, Messa, Letizia, Pandini, Cecilia, Maghraby, Erika, Barzaghini, Bianca, Garofalo, Maria, Micheletto, Giancarlo, Raimondi, Manuela Teresa, Bertoli, Simona, Cereda, Cristina, Zuccotti, Gian Vincenzo, Cancello, Raffaella, and Carelli, Stephana

Subjects
obesity, gender differences, lncRNAs, gene expression, Medicine, sex, computational prediction, bioinformatics, RNA-seq, metabolic diseases, transcriptome, Article
Abstract

Obesity is a multifactorial disease presenting sex-related differences including adipocyte functions, sex hormone effects, genetics, and metabolic inflammation. These can influence individuals' risk for metabolic dysfunctions, with an urgent need to perform sex-based analysis to improve prevention, treatment, and rehabilitation programs. This research work is aimed at characterizing the transcriptional differences present in subcutaneous adipose tissue (SAT) of five obesity affected men versus five obesity affected women, with an additional focus on the role of long non-coding RNAs. Through RNA-sequencing, we highlighted the presence of both coding and non-coding differentially expressed RNAs, and with numerous computational analyses we identified the processes in which these genes are implicated, along with their role in co-morbidities development. We report 51 differentially expressed transcripts, 32 of which were coding genes and 19 were non-coding. Using the WGCNA R package (Weighted Correlation Network Analysis, version 1.70-3), we describe the interactions between coding and non-coding RNAs, and the non-coding RNAs association with the insurgence of specific diseases, such as cancer development, neurodegenerative diseases, and schizophrenia. In conclusion, our work highlights a specific gender sex-related transcriptional signature in the SAT of obesity affected patients.

Published
2021

30. LncRNAs Associated with Neuronal Development and Oncogenesis Are Deregulated in SOD1-G93A Murine Model of Amyotrophic Lateral Sclerosis

Author

Rey, F, Marcuzzo, S, Bonanno, S, Bordoni, M, Giallongo, T, Malacarne, C, Cereda, C, Zuccotti, G, Carelli, S, Rey, Federica, Marcuzzo, Stefania, Bonanno, Silvia, Bordoni, Matteo, Giallongo, Toniella, Malacarne, Claudia, Cereda, Cristina, Zuccotti, Gian Vincenzo, Carelli, Stephana, Rey, F, Marcuzzo, S, Bonanno, S, Bordoni, M, Giallongo, T, Malacarne, C, Cereda, C, Zuccotti, G, Carelli, S, Rey, Federica, Marcuzzo, Stefania, Bonanno, Silvia, Bordoni, Matteo, Giallongo, Toniella, Malacarne, Claudia, Cereda, Cristina, Zuccotti, Gian Vincenzo, and Carelli, Stephana

Abstract

Amyotrophic Lateral Sclerosis (ALS) is a devastating neurodegenerative disease caused in 10% of cases by inherited mutations considered “familial”. An ever-increasing amount of evidence is showing a fundamental role for RNA metabolism in ALS pathogenesis, and long non-coding RNAs (lncRNAs) appear to play a role in ALS development. Here, we aim to investigate the expression of a panel of lncRNAs (linc-Enc1, linc–Brn1a, linc–Brn1b, linc-p21, Hottip, Tug1, Eldrr, and Fendrr) which could be implicated in early phases of ALS. Via Real-Time PCR, we assessed their expression in a murine familial model of ALS (SOD1-G93A mouse) in brain and spinal cord areas of SOD1-G93A mice in comparison with that of B6.SJL control mice, in asymptomatic (week 8) and late-stage disease (week 18). We highlighted a specific area and pathogenetic-stage deregulation in each lncRNA, with linc-p21 being deregulated in all analyzed tissues. Moreover, we analyzed the expression of their human homologues in SH-SY5Y-SOD1-WT and SH-SY5Y-SOD1-G93A, observing a profound alteration in their expression. Interestingly, the lncRNAs expression in our ALS models often resulted opposite to that observed for the lncRNAs in cancer. These evidences suggest that lncRNAs could be novel disease-modifying agents, biomarkers, or pathways affected by ALS neurodegeneration.

Published
2021

31. Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

Author

Rey, Federica, Messa, Letizia, Pandini, Cecilia, Launi, Rossella, Barzaghini, Bianca, Micheletto, Giancarlo, Raimondi, Manuela Teresa, Bertoli, Simona, Cereda, Cristina, Zuccotti, Gian Vincenzo, Cancello, Raffaella, and Carelli, Stephana

Subjects
Adult, Male, Transcription, Genetic, lncRNAs, Subcutaneous Fat, Article, lcsh:Chemistry, Open Reading Frames, Neoplasms, gender, cancer, Humans, Obesity, lcsh:QH301-705.5, Sex Characteristics, Gene Expression Profiling, Oncogenes, Prognosis, lcsh:Biology (General), lcsh:QD1-999, Diabetes Mellitus, Type 2, Gene Expression Regulation, transcriptional deregulation, Female, RNA, Long Noncoding, type 2 diabetes, Signal Transduction
Abstract

Obesity is a major risk factor for a large number of secondary diseases, including cancer. Specific insights into the role of gender differences and secondary comorbidities, such as type 2 diabetes (T2D) and cancer risk, are yet to be fully identified. The aim of this study is thus to find a correlation between the transcriptional deregulation present in the subcutaneous adipose tissue of obese patients and the oncogenic signature present in multiple cancers, in the presence of T2D, and considering gender differences. The subcutaneous adipose tissue (SAT) of five healthy, normal-weight women, five obese women, five obese women with T2D and five obese men were subjected to RNA-sequencing, leading to the identification of deregulated coding and non-coding RNAs, classified for their oncogenic score. A panel of DE RNAs was validated via Real-Time PCR and oncogene expression levels correlated the oncogenes with anthropometrical parameters, highlighting significant trends. For each analyzed condition, we identified the deregulated pathways associated with cancer, the prediction of possible prognosis for different cancer types and the lncRNAs involved in oncogenic networks and tissues. Our results provided a comprehensive characterization of oncogenesis correlation in SAT, providing specific insights into the possible molecular targets implicated in this process. Indeed, the identification of deregulated oncogenes also in SAT highlights hypothetical targets implicated in the increased oncogenic risk in highly obese subjects. These results could shed light on new molecular targets to be specifically modulated in obesity and highlight which cancers should receive the most attention in terms of better prevention in obesity-affected patients.

Published
2020

32. RNA-seq Characterization of Sex-Differences in Adipose Tissue of Obesity Affected Patients: Computational Analysis of Differentially Expressed Coding and Non-Coding RNAs

Author

Rey, Federica, primary, Messa, Letizia, additional, Pandini, Cecilia, additional, Maghraby, Erika, additional, Barzaghini, Bianca, additional, Garofalo, Maria, additional, Micheletto, Giancarlo, additional, Raimondi, Manuela Teresa, additional, Bertoli, Simona, additional, Cereda, Cristina, additional, Zuccotti, Gian Vincenzo, additional, Cancello, Raffaella, additional, and Carelli, Stephana, additional

Published
2021
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34. Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

Author

Rey, Federica, primary, Messa, Letizia, additional, Pandini, Cecilia, additional, Launi, Rossella, additional, Barzaghini, Bianca, additional, Micheletto, Giancarlo, additional, Raimondi, Manuela Teresa, additional, Bertoli, Simona, additional, Cereda, Cristina, additional, Zuccotti, Gian Vincenzo, additional, Cancello, Raffaella, additional, and Carelli, Stephana, additional

Published
2021
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36. Neural precursors cells expanded in a 3D micro-engineered niche present enhanced therapeutic efficacy in vivo

Author

Carelli, Stephana, primary, Giallongo, Toniella, additional, Rey, Federica, additional, Barzaghini, Bianca, additional, Zandrini, Tommaso, additional, Pulcinelli, Andrea, additional, Nardomarino, Riccardo, additional, Cerullo, Giulio, additional, Osellame, Roberto, additional, Cereda, Cristina, additional, Zuccotti, Gian Vincenzo, additional, and Raimondi, Manuela Teresa, additional

Published
2021
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37. Alzheimer’s, Parkinson’s Disease and Amyotrophic Lateral Sclerosis Gene Expression Patterns Divergence Reveals Different Grade of RNA Metabolism Involvement

Author

Garofalo, Maria, primary, Pandini, Cecilia, additional, Bordoni, Matteo, additional, Pansarasa, Orietta, additional, Rey, Federica, additional, Costa, Alfredo, additional, Minafra, Brigida, additional, Diamanti, Luca, additional, Zucca, Susanna, additional, Carelli, Stephana, additional, Cereda, Cristina, additional, and Gagliardi, Stella, additional

Published
2020
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38. Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Author

Rey, Federica, primary, Pandini, Cecilia, additional, Barzaghini, Bianca, additional, Messa, Letizia, additional, Giallongo, Toniella, additional, Pansarasa, Orietta, additional, Gagliardi, Stella, additional, Brilli, Matteo, additional, Zuccotti, Gian Vincenzo, additional, Cereda, Cristina, additional, Raimondi, Manuela Teresa, additional, and Carelli, Stephana, additional

Published
2020
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40. A New Selective PPARγ Modulator Inhibits Triglycerides Accumulation during Murine Adipocytes’ and Human Adipose-Derived Mesenchymal Stem Cells Differentiation

Author

Al Haj, Ghina, primary, Rey, Federica, additional, Giallongo, Toniella, additional, Colli, Mattia, additional, Marzani, Barbara, additional, Giuliani, Giammaria, additional, Gorio, Alfredo, additional, Zuccotti, Gian Vicenzo, additional, Di Giulio, Anna Maria, additional, and Carelli, Stephana, additional

Published
2020
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42. Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities

Author

Rey, Federica, Lesma, Elena, Massihnia, Daniela, Ciusani, Emilio, Nava, Sara, Vasco, Chiara, Al Haj, Ghina, Ghilardi, Giorgio, Opocher, Enrico, Gorio, Alfredo, Carelli, Stephana, and Di Giulio, Anna Maria

Subjects
lcsh:Internal medicine, Article Subject, lcsh:RC31-1245, Research Article
Abstract

Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into multiple cell types, including adipocytes, osteoblasts, and chondrocytes. The role of adipose-derived stem cells (ADSCs) in cancers is significantly relevant. They seem to be involved in the promotion of tumour development and progression and relapse processes. For this reason, investigating the effects of breast cancer microenvironment on ADSCs is of high importance in order to understand the relationship between tumour cells and the surrounding stromal cells. With the current study, we aimed to investigate the specific characteristics of human ADSCs isolated from the adipose tissue of breast tumour patients. We compared ADSCs obtained from periumbilical fat (PF) of controls with ADSCs obtained from adipose tissue of breast cancer- (BC-) bearing patients. We analysed the surface antigens and the adipogenic differentiation ability of both ADSC populations. C/EBPδ expression was increased in PF and BC ADSCs induced to differentiate compared to the control while PPARγ and FABP4 expressions were enhanced only in PF ADSCs. Conversely, adiponectin expression was reduced in PF-differentiated ADSCs while it was slightly increased in differentiated BC ADSCs. By means of Oil Red O staining, we further observed an impaired differentiation capability of BC ADSCs. To investigate this aspect more in depth, we evaluated the effect of selective PPARγ activation and nutritional supplementation on the differentiation efficiency of BC ADSCs, noting that it was only with a strong differentiation stimuli that the process took place. Furthermore, we observed no response in BC ADSCs to the PPARγ inhibitor T0070907, showing an impaired activation of this receptor in adipose cells surrounding the breast cancer microenvironment. In conclusion, our study shows an impaired adipogenic differentiation capability in BC ADSCs. This suggests that the tumour microenvironment plays a key role in the modulation of the adipose microenvironment located in the surrounding tissue.

Published
2019

43. Role of epigenetics and alterations in RNAmetabolism in leukodystrophies

Author

Rey, Federica, Esposito, Letizia, Maghraby, Erika, Mauri, Alessia, Berardo, Clarissa, Bonaventura, Eleonora, Tonduti, Davide, Carelli, Stephana, and Cereda, Cristina

Abstract

Leukodystrophies are a class of rare heterogeneous disorders which affect the white matter of the brain, ultimately leading to a disruption in brain development and a damaging effect on cognitive, motor and social‐communicative development. These disorders present a great clinical heterogeneity, along with a phenotypic overlap and this could be partially due to contributions from environmental stimuli. It is in this context that there is a great need to investigate what other factors may contribute to both disease insurgence and phenotypical heterogeneity, and novel evidence are raising the attention toward the study of epigenetics and transcription mechanisms that can influence the disease phenotype beyond genetics. Modulation in the epigenetics machinery including histone modifications, DNA methylation and non‐coding RNAs dysregulation, could be crucial players in the development of these disorders, and moreover an aberrant RNA maturation process has been linked to leukodystrophies. Here, we provide an overview of these mechanisms hoping to supply a closer step toward the analysis of leukodystrophies not only as genetically determined but also with an added level of complexity where epigenetic dysregulation is of key relevance. This article is categorized under:Regulatory RNAs/RNAi/Riboswitches > Regulatory RNARNA in Disease and Development > RNA in DiseaseRNA in Disease and Development > RNA in Development Leukodystrophies are rare brain disorders affecting white matter, with a clinical diversity still to be explained. Beyond genetics, epigenetic factors like histone modifications, DNA methylation, and non‐coding RNA dysregulation, as well as aberrant RNA maturation, are emerging as crucial contributors to these disorders. Created with Biorender.com

Published
2024
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45. Additional file 3: of Counteracting neuroinflammation in experimental Parkinson’s disease favors recovery of function: effects of Er-NPCs administration

Author

Stephana Carelli, Toniella Giallongo, Gombalova, Zuzana, Rey, Federica, Gorio, Maria, Mazza, Massimiliano, and Giulio, Anna

Abstract

Percentage of cells positive to CD11b, GFAP, and Iba1. Graphs report the stereologic counts of positive cells to investigated markers quantified in nine different fields for each condition (three mice for each group). The analysis was performed 2 weeks after Er-NPCs injection. Quantification was done by ImageJ picture analysis software. Data are expressed as mean ± SD. ***p

Published
2018
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46. Additional file 4: of Counteracting neuroinflammation in experimental Parkinson’s disease favors recovery of function: effects of Er-NPCs administration

Author

Stephana Carelli, Toniella Giallongo, Gombalova, Zuzana, Rey, Federica, Gorio, Maria, Mazza, Massimiliano, and Giulio, Anna

Abstract

Percentage of cells positive to CD68, CD86, and CD206. Graphs report the stereological counts of positive cells to the investigated markers quantified in nine different fields for each condition (three mice for each group). The analyses were performed by evaluating nine different fields for each condition (three mice for each group). Quantification was done by ImageJ picture analysis software. Data are expressed as mean ± SD. ***p

Published
2018
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47. Additional file 2: of Counteracting neuroinflammation in experimental Parkinson’s disease favors recovery of function: effects of Er-NPCs administration

Author

Stephana Carelli, Toniella Giallongo, Gombalova, Zuzana, Rey, Federica, Gorio, Maria, Mazza, Massimiliano, and Giulio, Anna

Subjects
nervous system, animal diseases, otorhinolaryngologic diseases, cardiovascular system, nervous system diseases
Abstract

Er-NPC-treated animals recover olfactory capabilities. Five groups of animals were analyzed with this test: 1) Control (CTRL, healthy animals, n = 24); 2) MPTP treated mice (MPTP, n = 24); 3) MPTP treated mice infused with PBS (SHAM, sham operated; n = 18); 4) MPTP treated mice transplanted with Er-NPCs (MPTP + Er-NPCs, n = 24) 5) MPTP treated mice transplanted with Er-NPCs and anti-erythropoietin antibody (MPTP + Er-NPCs + aEPO, n = 12). Data are expressed as mean ± SD. Statistical analysis was performed with two-way ANOVA test followed by Bonferroni post-test. °p

Published
2018
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