459 results on '"Rezaei‐Matehkolaei, A."'
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2. Aspergillus hubkae, a Novel Species Isolated from a Patient with Probable Invasive Pulmonary Aspergillosis
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Zhou, Ya Bin, Rezaei-Matehkolaei, Ali, Meijer, Martin, Kraak, Bart, Gerrits van den Ende, Bert, Hagen, Ferry, Afzalzadeh, Sara, Kiasat, Neda, Takesh, Ameneh, Hoseinnejad, Akbar, and Houbraken, Jos
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- 2024
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3. Trends in Epidemiology of Dermatophytes in Iran
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Gharaghani, Maral, Taghipour, Simin, Shamsizadeh, Forough, Abastabar, Mahdi, Ansari, Saham, Heidari, Ahmad, Zarei-Mahmoudabadi, Ali, Rezaei-Matehkolaei, Ali, Bouchara, Jean-Philippe, editor, Nenoff, Pietro, editor, Gupta, Aditya K., editor, and Chaturvedi, Vishnu, editor
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- 2021
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4. A simple multiplex polymerase chain reaction assay for rapid identification of the common pathogenic dermatophytes:Trichophyton interdigitale, Trichophyton rubrum, and Epidermophyton floccosum
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Sama Faramarzi, Marjan Motamedi, Ali Rezaei-Matehkolaei, Shima Aboutalebian, Saham Ansari, Mojtaba Didehdar, Mehran Bahadoran, and Hossein Mirhendi
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e. floccosum ,t. interdigitale/t. mentagrophytes ,t. rubrum ,dermatophyte ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Background and Purpose: The most common etiological agents of human dermatophytosis in various parts of the world are Trichophyton rubrum, Trichophyton interdigitale, and Epidermophyton floccosum. The main aim of this study was to design and evaluate a simple and straightforward multiplex polymerase chain reaction (PCR)assay for reliable identification/differentiation of these species in clinical isolates.Materials and Methods: The reliable sequences of several molecular targets of dermatophytes species were used to design a multiplex PCR for the identification of common pathogenic dermatophytes. The isolates and clinical specimens examined in this study included seven standard strains of dermatophytes, 101 isolates of dermatophytes and non-dermatophyte molds/yeasts which had already been identified by sequencing or PCR-restriction fragment length polymorphism (RFLP), and 155 clinical samples from patients suspected of cutaneous mycoses.Results: Species-specific primer pairs for T. rubrum and T. interdigitale/T. mentagrophytes were designed based on the sequence data of the translation elongation factor 1-alpha gene, and the primers for E. floccosum targeted the specific sequence of the internal transcribed spacer region (ITS). The multiplex PCR successfully detected T.rubrum, T. interdigitale/T. mentagrophytes, and E. floccosum strains that were identified by sequencing or PCR-RFLP. However, the primer pairs selected for T. interdigitale/T. mentagrophytes cross-reacted with Trichophyton tonsurans. In testing the PCR system directly for clinical samples, the proportion of positive multiplex PCR was higher than positive culture (68.1% vs. 55.4%, respectively).Conclusion: The multiplex assay could detect three common agents out of several causal agents of dermatophytosis, namely T. rubrum, T. interdigitale, and E. floccosum.Therefore, by adding pan-dermatophyte primers it can be used as a comprehensive detection/identification test.
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- 2021
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5. Potential Inhibitory Effect of Miltefosine against Terbinafine-Resistant Trichophyton indotineae
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Iman Haghani, Javad Akhtari, Zahra Yahyazadeh, Amirreza Espahbodi, Firoozeh Kermani, Javad Javidnia, Mohammad Taghi Hedayati, Tahereh Shokohi, Hamid Badali, Ali Rezaei-Matehkolaei, Seyed Reza Aghili, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Mahdi Abastabar, and Abdullah M. S. Al-Hatmi
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miltefosine ,T. mentagrophytes ,T. interdigitale ,T. indotineae ,terbinafine ,Medicine - Abstract
Several prolonged and significant outbreaks of dermatophytosis caused by Trichophyton indotineae, a new emerging terbinafine-resistant species, have been ongoing in India in recent years, and have since spread to various countries outside Asia. Miltefosine, an alkylphosphocholine, is the most recently approved drug for the treatment of both visceral and cutaneous leishmaniasis. Miltefosine in vitro activity against terbinafine-resistant and susceptible T. mentagrophytes/T. interdigitale species complex, including T. indotineae, is limited. The current study aimed to assess miltefosine’s in vitro activity against dermatophyte isolates, which are the most common causes of dermatophytosis. Miltefosine, terbinafine, butenafine, tolnaftate, and itraconazole susceptibility testing was performed using Clinical and Laboratory Standards Institute broth microdilution methods (CLSI M38-A3) against 40 terbinafine-resistant T. indotineae isolates and 40 terbinafine-susceptible T. mentagrophytes/T. interdigitale species complex isolates. Miltefosine had MIC ranges of 0.063–0.5 µg/mL and 0.125–0.25 µg/mL against both terbinafine-resistant and susceptible isolates. In terbinafine-resistant isolates, the MIC50 and MIC90 were 0.125 µg/mL and 0.25 µg/mL, respectively, and 0.25 µg/mL in susceptible isolates. Miltefosine had statistically significant differences in MIC results when compared to other antifungal agents (p-value 0.05) in terbinafine-resistant strains. Accordingly, the findings suggest that miltefosine has a potential activity for treating infections caused by terbinafine-resistant T. indotineae. However, further studies are needed to determine how well this in vitro activity translates into in vivo efficacy.
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- 2023
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6. Familial Cases of Trichophyton benhamiae Infection Transmitted from a Guinea Pig in Iran
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Ansari, Saham, Ahmadi, Bahram, Tabatabaeifar, Seyedeh Nadia, Hedayati, Mohammad T., Javidnia, Javad, Taghizadeh Armaki, Mojtaba, Shokoohi, Gholam Reza, and Rezaei-Matehkolaei, Ali
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- 2021
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7. Molecular typing of a collection of Iranian clinical Trichophyton tonsurans isolates based on the non‐transcribed spacer region of rDNA and antifungal susceptibility testing of the species
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Faeli, Leila, primary, Kermani, Firoozeh, additional, Rezaei‐Matehkolaei, Ali, additional, Ilkit, Macit, additional, Valadan, Reza, additional, Hosseini, Seyed Abdollah, additional, Javidnia, Javad, additional, Mayahi, Sabah, additional, Shokohi, Tahereh, additional, and Abastabar, Mahdi, additional
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- 2023
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8. Phylogenetic Analysis of Clinically Relevant Fusarium Species in Iran
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Najafzadeh, Mohammad Javad, Dolatabadi, Somayeh, de Hoog, Sybren, Esfahani, Mahmoud Karimizadeh, Haghani, Iman, Aghili, Seyed Reza, Ghazvini, Roshanak Daei, Rezaei-Matehkolaei, Ali, Abastabar, Mahdi, and Al-Hatmi, Abdullah M. S.
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- 2020
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9. Discovery of New Trichophyton Members, T. persicum and T. spiraliforme spp. nov., as a Cause of Highly Inflammatory Tinea Cases in Iran and Czechia
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Adéla Čmoková, Ali Rezaei-Matehkolaei, Ivana Kuklová, Miroslav Kolařík, Forough Shamsizadeh, Saham Ansari, Maral Gharaghani, Viera Miňovská, Mohammad Javad Najafzadeh, Sadegh Nouripour‐Sisakht, Takashi Yaguchi, Kamiar Zomorodian, Hossein Zarrinfar, and Vit Hubka
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dermatophytosis ,molecular epidemiology ,multigene phylogeny ,skin mycoses ,Trichophyton benhamiae complex ,zoonotic infections ,Microbiology ,QR1-502 - Abstract
ABSTRACT Pathogens from the Trichophyton benhamiae complex are one of the most important causes of animal mycoses with significant zoonotic potential. In light of the recently revised taxonomy of this complex, we retrospectively identified 38 Trichophyton isolates that could not be resolved into any of the existing species. These strains were isolated from Iranian and Czech patients during molecular epidemiological surveys on dermatophytosis and were predominantly associated with highly inflammatory tinea corporis cases, suggesting possible zoonotic etiology. Subsequent phylogenetic (4 markers), population genetic (10 markers), and phenotypic analyses supported recognition of two novel species. The first species, Trichophyton persicum sp. nov., was identified in 36 cases of human dermatophytosis and one case of feline dermatophytosis, mainly in Southern and Western Iran. The second species, Trichophyton spiraliforme sp. nov., is only known from a single case of tinea corporis in a Czech patient who probably contracted the infection from a dog. Although the zoonotic sources of infections summarized in this study are very likely, little is known about the host spectrum of these pathogens. Awareness of these new pathogens among clinicians should refine our knowledge about their poorly explored geographic distribution. IMPORTANCE In this study, we describe two novel agents of dermatophytosis and summarize the clinical manifestation of infections. These new pathogens were discovered thanks to long-term molecular epidemiological studies conducted in Czechia and Iran. Zoonotic origins of the human infections are highly probable, but the animal hosts of these pathogens are poorly known. Further research is needed to refine our knowledge about these new dermatophytes.
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- 2021
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10. The growth inhibitory and apoptotic effects of umbelliprenin in a mouse model of systemic candidiasis
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Rashidi, Mohsen, primary, Bazi, Ali, additional, Ahmadzadeh, Alireza, additional, Romeo, Orazio, additional, Rezaei-Matehkolaei, Ali, additional, Abastabar, Mahdi, additional, Haghani, Iman, additional, and Mirzaei, Sepideh, additional
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- 2023
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11. Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase Chain Reaction Sequencing (PCR Sequencing) Method
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Raheleh Nejati-Hoseini, Hossein Zarrinfar, Mahmoud Parian, Saeid Parham, Abdolmajid Fata, Ali Rezaei-Matehkolaei, and Mohammad Javad Najafzadeh
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Dermatophytosis ,DNA sequencing ,Iran ,Medicine ,Medicine (General) ,R5-920 - Abstract
Background: Dermatophytes are a group of fungi that attack keratinous tissues of the skin, hair, and nail in humans and animals, and cause infections called dermatophytosis (tinea). Since identification of pathogenic fungi at the species level is essential for the detection of the source, control and prevention, and identifying epidemiology of infection, it is necessary to use specific and sensitive diagnostic methods to identify the causes of dermatophytosis. Methods: The clinical samples (skin, nail, and hair) of patients with dermatophytosis in Mashhad City, Iran, were cultured in Mycosyl Agar culture media, and the DNA of obtained dermatophyte colonies were extracted by specific kit. The internal transcribed spacer (ITS) gene was amplified and sequenced by ITS1, ITS4 primers. Finally, the sequencing results were analyzed using SeqMan software, and were compared with the data of the global genebank. Findings: In this study, 80 dermatophyte isolates were sequenced, which included 9 dermatophyte species as 23 (28.8%) Trichophyton (T.) interdigital, 18 (22.5%) T. tunsorans, 10 (12.5%) Epidermophyton fluccosum, 10 (12.5%) of T. mentagrophytes, 8 (10%) Microsporum canis, 4 (5%) T. rubrum, 4 (5%) T. benhamiae, 2 (2.5%) Nannizzia (N.) fulvum, 1 (1.2%) N. persicolor. Conclusion: According to report the rare species of dermatophytes in this study, the use of molecular methods such as sequencing of the ITS gene can determine the diversity of dermatophytes in a region more precisely than morphological methods.
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- 2019
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12. Trichophyton indotineae—An Emerging Pathogen Causing Recalcitrant Dermatophytoses in India and Worldwide—A Multidimensional Perspective
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Silke Uhrlaß, Shyam B. Verma, Yvonne Gräser, Ali Rezaei-Matehkolaei, Maryam Hatami, Martin Schaller, and Pietro Nenoff
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dermatophytoses ,tinea corporis ,tinea cruris ,tinea faciei ,terbinafine resistance ,Itraconazole ,Biology (General) ,QH301-705.5 - Abstract
Trichophyton (T.) indotineae is a newly identified dermatophyte species that has been found in a near-epidemic form on the Indian subcontinent. There is evidence of its spread from the Indian subcontinent to a number of countries worldwide. The fungus is identical to genotype VIII within the T. mentagrophytes/T. interdigitale species complex, which was described in 2019 by sequencing the Internal Transcribed Spacer (ITS) region of ribosomal DNA of the dermatophyte. More than 10 ITS genotypes of T. interdigitale and T. mentagrophytes can now be identified. T. indotineae causes inflammatory and itchy, often widespread, dermatophytosis affecting the groins, gluteal region, trunk, and face. Patients of all ages and genders are affected. The new species has largely displaced other previously prevalent dermatophytes on the Indian subcontinent. T. indotineae has become a problematic dermatophyte due to its predominantly in vitro genetic resistance to terbinafine owing to point mutations of the squalene epoxidase gene. It also displays in vivo resistance to terbinafine. The most efficacious drug currently available for this terbinafine-resistant dermatophytoses, based on sound evidence, is itraconazole.
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- 2022
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13. The patterns of colonization and antifungal susceptibility of Candida, isolated from preterm neonates in Khorramabad, South West of Iran
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Kooshki, P., Rezaei-Matehkolaei, A., and Mahmoudabadi, A.Z.
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- 2018
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14. Mucormycosis in Iran: A six-year retrospective experience
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Dolatabadi, S., Ahmadi, B., Rezaei-Matehkolaei, A., Zarrinfar, H., Skiada, A., Mirhendi, H., Nashibi, R., Niknejad, F., Nazeri, M., Rafiei, A., Gharaghani, M., Erami, M., Taghipour, S., Piri, F., and Makimura, K.
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- 2018
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15. Luliconazole, a new antifungal against Candida species isolated from different sources
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Taghipour, S., Kiasat, N., Shafiei, S., Halvaeezadeh, M., Rezaei-Matehkolaei, A., and Zarei Mahmoudabadi, A.
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- 2018
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16. Isolation and antifungal activity evaluation of Satureja khuzestanica Jamzad extract against some clinically important dermatophytes
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Sadeghi-Nejad, B., Rezaei-Matehkolaei, A., and Yusef Naanaie, S.
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- 2017
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17. Identification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS
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Majid Alizadeh, Anna Kolecka, Teun Boekhout, Hossein Zarrinfar, Mohamad Ali Ghanbari Nahzag, Parisa Badiee, Ali Rezaei-Matehkolaei, Abdolmajid Fata, Somayeh Dolatabadi, and Mohammad Javad Najafzadeh
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MALDI-TOF MS ,Candidiasis ,Vulvovaginitis ,identification ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Background and Purpose Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify of Candida species isolated from vulvovaginitis woman suffering vulvovaginitis refered to Ghaem Hospital, Mashhad, Iran, by use of MALDI-TOF mass spectrometry. Materials and Methods The 65 clinical samples isolated from Vulvovaginitis women were collected in Ghaem Hospital. All specimens were identified using phenotypic techniques such as microscopy and culture on Sabouraud dextrose agar and corn meal agar medium,Then, All isolates were detected and were processed for MALDI TOF MS identification. Results Of the 65 isolates analyzed, 61 (93.8%) were recognised by MALDI-TOF mass spectrometry and for four isolates (6.1%) only not relabile identifications were achieved. In this study, the most frequently isolated species were Candida albicans (58.5%), followed by Candida tropicalis (16.9%), Candida glabrata (7.7%), Candida parapsilosis (7.7%) and Candida guillermondii (3.1%). Conclusion presented results demonstrate that the MALDI TOF mass spectrometry is a fast and reliable technique, and has the potential to replace conventional phenotypic identification of Candida species and other yeast strains routinely isolated in clinical microbiology laboratories.
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- 2017
18. Mycological Aspects of Onychomycosis in Khuzestan Province, Iran, a New Scenario as Shift from Dermatophytes towards Yeasts
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Mahnaz fatahinia, Sima Jafarpour, Abdollah Rafiei, Simin Taghipour, Koichi Makimura, and Ali Rezaei-Matehkolaei
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Onychomycosis ,Khuzestan ,Yeast ,Deramatophyte ,NDM. ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background and Purpose: Onychomycosis is fungal infection of the nails with an overall increasing incidence, worldwide. The epidemiological aspects of onychomycosis in Khuzestan, Iran have not been established. This study was aimed to evaluate the clinical and mycological status of fungal nail infection in Khuzestan Province, Iran. Material and Methods: The study population included 433 patients (143 males vs. 290 females). Nail samples were undergone to primary direct microscopy and culture. The isolated yeasts and dermatophytes were then subjected to additional molecular identification by r-DNA ITS-RFLP. Identification of some non-dermatophytes molds (NDMs) and unknown yeasts were accomplished by ITS and beta tubulin sequencing. Results: Onychomycosis was confirmed in 154 patients (35.6%; 58 males vs. 96 females) whose age ranged from 2-85 years with highest prevalence in the age group of 41-50 years old. Infection was more occurred due to yeasts (59.7%) with Candida albicans as the most frequent (29.35%) species, followed by C. parapsilosis (13.8%) and C. tropicalis (4.5%). Dermatophytes were isolated in 38.35% of the cases; the most commonly isolates were found to be Trichophyton interdigitale (21.1%), Epidermophyton floccosum (10.5%), T. rubrum (5.25%) and Microsporum canis (1.5%). NDMs were isolated only in 4.5% with Aspergillus spp., as the commonest agents. Dermatophytes and NDMs more frequently seen in toenails whereas, yeasts mostly infected fingernails and fingernail onychomycosis has remarkably more occurred in females than males (P < 0.05). Conclusion: The study highlighted that the agents of infection in the population with onychomycosis from Khuzestan have shifted from dermatophytes to the yeasts.
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- 2017
19. Luliconazole, an alternative antifungal agent against Aspergillus terreus
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Zargaran, M., Taghipour, S., Kiasat, N., Aboualigalehdari, E., Rezaei-Matehkolaei, A., Zarei Mahmoudabadi, A., and Shamsizadeh, F.
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- 2017
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20. Comparison of enzymatic activities in different Candida species isolated from women with vulvovaginitis
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Fatahinia, M., Halvaeezadeh, M., and Rezaei-Matehkolaei, A.
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- 2017
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21. Time and cost‐efficient identification of Trichophyton indotineae
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Abolfazl Batvandi, Ivan M. Pchelin, Neda Kiasat, Mahboobeh Kharazi, Rasoul Mohammadi, Kamiar Zomorodian, and Ali Rezaei‐Matehkolaei
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Infectious Diseases ,Trichophyton ,Tinea ,Humans ,Dermatology ,General Medicine ,DNA, Fungal ,DNA, Ribosomal ,Polymerase Chain Reaction ,Polymorphism, Restriction Fragment Length - Abstract
During the past 5 years, an outbreak of recalcitrant dermatophytosis due to a novel Trichophyton species generally resistant to terbinafine, T. indotineae, has spread out from South Asia to many countries around the World. These isolates cannot be reliably differentiated from other Trichophyton spp. on the basis of morphological traits and the current laboratory diagnostics relies on sequencing of ribosomal DNA ITS region.In this study, we aimed to introduce two inexpensive and rapid PCR-based assays for differentiation between T. indotineae and other dermatophytes.The first introduced assay is based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, involving the amplification of TOP2 sequences and the digestion of PCR products by Cfr13I restriction enzyme. The second assay is proposed as conventional endpoint species-specific PCR amplification of the C120-287 intergenic locus. To validate the assays, a total of 191 Trichophyton spp. and 2 Microsporum canis strains with known ITS region sequences were used. From the T. mentagrophytes / T. interdigitale species complex (TMTISC), strains with 18 different ITS genotypes were tested. The sample of TMTISC isolates included 41 T. indotineae strains.TOP2 PCR-RFLP and T. indotineae-specific PCR were positive with testing on DNA of all 41 T. indotineae isolates and two strains of T. mentagrophytes belonging to ITS Types XIII and XVI, but negative with other species and other TMTISC ITS genotypes (n = 152). Therefore, the specificity of both new assays was 99%.The two developed diagnostic assays provide accurate and cost-effective means of identifying cultured T. indotineae isolates.
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- 2022
22. Molecular typing of a collection of Iranian clinical Trichophyton tonsurans isolates based on the non-transcribed spacer region of rDNA and antifungal susceptibility testing of the species.
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Faeli, Leila, Kermani, Firoozeh, Rezaei-Matehkolaei, Ali, Ilkit, Macit, Valadan, Reza, Hosseini, Seyed Abdollah, Javidnia, Javad, Mayahi, Sabah, Shokohi, Tahereh, and Abastabar, Mahdi
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TRICHOPHYTON ,GENETIC variation ,RECOMBINANT DNA ,IRANIANS ,HAPLOTYPES ,ANTIFUNGAL agents ,ITRACONAZOLE - Abstract
Introduction: Wrestling, considered the national sport of Iran, has gained immense popularity among Iranians. Wrestlers frequently encounter skin conditions, with dermatophyte fungal infections, particularly tinea gladiatorum (TG), being a common issue. TG, caused by the Trichophyton genus, has emerged as a major health concern for wrestlers and other contact sport athletes worldwide. This study aimed to assess the genotypic diversity and antifungal susceptibility of Trichophyton tonsurans isolates responsible for TG in Iranian wrestlers from Mazandaran province, northern Iran. Materials and Methods: A total of 60 clinical T. tonsurans isolates collected from various cities in Mazandaran, were included in the study. The isolates were identified through PCR-restriction fragment length polymorphism and sequencing methods. Genomic DNA was extracted from these isolates, and the non-transcribed spacer (NTS) region of ribosomal RNA (rRNA) was targeted for genotyping using newly designed primers. Haplotype analysis was performed to explore genetic diversity, and antifungal susceptibility to terbinafine (TRB) and itraconazole (ITC) was assessed. Results: The results revealed five distinct NTS types: NTS-I, NTS-II, NTS-III, NTS-IV and NTS-V, with NTS-IV being the most prevalent. The distribution of NTS types varied across different cities, suggesting potential transmission patterns among wrestlers. Antifungal susceptibility testing showed that all isolates were susceptible to TRB, while one isolate demonstrated resistance to ITC. Genotypic diversity was not correlated with antifungal susceptibility, emphasising the importance of monitoring susceptibility to ensure effective treatment. Haplotype analysis highlighted significant genetic diversity among the T. tonsurans isolates. This diversity may be attributed to factors such as human-to-human transmission, geographic location and lifestyle changes. The study's findings underscore the need for comprehensive genotypic analysis to understand the epidemiology and evolution of T. tonsurans infections in athletes. Conclusion: In conclusion, this study provides valuable insights into the genotypic diversity and antifungal susceptibility of T. tonsurans isolates causing TG in Iranian wrestlers. The presence of multiple NTS types and varying susceptibility patterns highlights the complexity of T. tonsurans infections in this population. Further research is warranted to track the transmission routes and genetic evolution of T. tonsurans strains among wrestlers and develop effective control measures. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes
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Najafzadeh, M. J., Vicente, V. A., Feng, Peiying, Naseri, A., Sun, Jiufeng, Rezaei-Matehkolaei, A., and de Hoog, G. S.
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- 2018
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24. Epidemiological Status of Dermatophytosis in Guilan, North of Iran
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Ali Akbar Fallahi, Ali Rezaei-Matehkolaei, and Sasan Rezaei
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Dermatophyte ,Epidemiology ,Guilan ,Tinea ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Background and Purpose: The epidemiological features of dermatophytoses have been characterized in many geographical locations of Iran, but not in Guilan, North of Iran. This study was carried out to determine the distribution pattern of dermatophytoses and their relevant agents in Guilan, North of Iran, over a period of one year, from April 2010 to April 2011. Materials and Methods: The clinical samples of skin, hair, and nail from 889 outpatients (317 men vs. 572 women) were used for direct microscopy and culture. All the culture-positive samples were then subjected to amplification of the internal transcribed spacer (ITS) of the nuclear rDNA followed by a restriction fragment length polymorphism (RFLP) assay to verify the causative agents. Results: The infection was confirmed in 90 (44.3%) males and 113 (55.7%) females.The most common type of dermatophytoses was tinea cruris (42.9%), followed by tinea pedis (20.2%), tinea corporis (11.3%), tinea unguium (7.4%), tinea faciei (6.9%), tinea manuum (6.4%), and tinea capitis (4.9%). ITS-RFLP based of the identification of isolates, showed that the infections were significantly associated with anthropophilic species, of Trichophyton rubrum (41.9%), Epidermophyton floccosum (19.7%), T.tonsurans (5.4%), and T. violaceum (2%). Other causative agents were T. interdigitale (22.6%),Microsporum canis (4.9%), T. verrucosum (2.5%), and M. gypseum. (1%). Conclusion: The higher prevalence of T. rubrum, as the agent of dermatophytoses, than other species has never been reported from Iran and is of public health concern because of the chronic nature of infections with anthropophilic species. To thoroughly investigate the epidemiological trend of dermatophytoses in Iran, further periodical and molecularbased studies are necessary.
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- 2017
25. Potential Inhibitory Effect of Miltefosine against Terbinafine-Resistant Trichophyton indotineae
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Haghani, Iman, primary, Akhtari, Javad, additional, Yahyazadeh, Zahra, additional, Espahbodi, Amirreza, additional, Kermani, Firoozeh, additional, Javidnia, Javad, additional, Hedayati, Mohammad Taghi, additional, Shokohi, Tahereh, additional, Badali, Hamid, additional, Rezaei-Matehkolaei, Ali, additional, Aghili, Seyed Reza, additional, Al-Rawahi, Ahmed, additional, Al-Harrasi, Ahmed, additional, Abastabar, Mahdi, additional, and Al-Hatmi, Abdullah M. S., additional
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- 2023
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26. Microsatellite Typing and Antifungal Susceptibility of Candida glabrata Strains Isolated From Patients With Candida Vaginitis
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Neda Kiasat, Ali Rezaei-Matehkolaei, and Ali Zarei Mahmoudabadi
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Candida glabrata ,vulvovaginal candidiasis ,antifungal susceptibility ,microsatellite genotyping ,Iran ,Microbiology ,QR1-502 - Abstract
Vulvovaginal candidiasis (VVC) is a yeast infection with a global reach and millions of dollars are spent annually for its diagnosis and treatment. Recently, Candida glabrata with different degrees of antifungal resistance has been considered as the second most common cause of vaginal infections. The aim of the present study is to determine the antifungal susceptibility and molecular epidemiology profiles of C. glabrata isolates from patients with VVC. Sixty-one C. glabrata isolates were examined for antifungal susceptibility using the EUCAST broth microdilution method. Moreover, microsatellite length polymorphism (MLP) was used for typing the C. glabrata isolates using six microsatellite markers. Overall, 13, 3.3, and 0% of the isolates were non-wild types to itraconazole, posaconazole, and voriconazole, respectively. Sixty (98.4%) isolates were an intermediate phenotype to fluconazole and only one isolate was fluconazole resistant. Microsatellite length polymorphism with a discriminatory power of 0.964 identified 35 distinct types and 24 singleton genotypes. The assessment of the population genetic structure revealed that the non-wild-type population had a moderate genetic differentiation compared to the wild type population (FST = 0.1457). It was also found that the most common genotypes were G27 (eight strains), G12 (six strains), and G4 (five strains). We found that eight strains were resistant/a non-wild phenotype to itraconazole. Five out of eight (62.5%) resistant/non-wild phenotype strains correlated to a predominant genotype (GT27) and the rest belonged to GT11 (12.5%), GT29 (12.5%), and GT28 (12.5%). The current study is the first molecular epidemiology study in the southwest of Iran and demonstrates the antifungal susceptibility profiles of C. glabrata in it. This study shows a wide range of the genetic diversity of C. glabrata (35 different genotypes) from VVC in the southwest of Iran. The majority of the non-wild isolates had a dominant genotype or genotypes related to this dominant genotype (clonal cluster one).
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- 2019
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27. Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
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Simin Taghipour, Mahdi Abastabar, Fahimeh Piri, Elham Aboualigalehdari, Mohammad Reza Jabbari, Hossein Zarrinfar, Sadegh Nouripour-Sisakht, Rasoul Mohammadi, Bahram Ahmadi, Saham Ansari, Farzad Katiraee, Farhad Niknejad, Mojtaba Didehdar, Mehdi Nazeri, Koichi Makimura, and Ali Rezaei-Matehkolaei
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geophilic dermatophytes ,Nannizzia fulva ,Arthroderma ,ITS sequencing ,Iran ,Biology (General) ,QH301-705.5 - Abstract
A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and MvaI restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1–8.0, collected from animal habitats (n = 78; 34%) and parks and gardens (n = 75; 32%), geographically from Mazandaran Province (n = 115; 49.5%) and seasonally in the spring (n = 129; 50.4%), all of which were statistically significant (p < 0.05). The dermatophytes comprising five species of the two genera, viz., Nannizzia fulva (n = 214), N. gypsea (n = 34), Arthroderma quadrifidum (n = 5), A. gertleri (n = 2) and A. tuberculatum (n = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, N. fulva emerged as the dominant species, outnumbering the common geophilic species of N. gypsea. For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended.
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- 2021
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28. The first case of onychomycosis in a koala (Phascolarctos cinereus) due to atypical isolates of Microsporum gypseum, a diagnostic challenge
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Hossein Mirhendi, Yayoi Nishiyama, Ali Rezaei-Matehkolaei, Kazuo Satoh, and Koichi Makimura
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Dermatophytosis ,Koala ,Microsporum gypseum ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Background and Purpose: Superficial mycotic infections have been only poorly described in koalas and there are no reliable mycologically confirmed data regarding clinical isolation of dermatophytes in this animal. We report an 11-yearold female koala, kept in a zoo in Tokyo, Japan, and presenting with hyperkeratotic lesions and scaly plaques on forepaw claws and pads reminiscent of fungal infection. Case Report: Direct microscopy of the scrapings was indicative of a dermatophyte infection. By culture and subsequent repeated subculturing of clinical specimens on Sabouraud dextrose agar, Mycobiotic agar, and potato dextrose agar, two distinct strains with different colony morphotypes (designed as types I and II) were identified. Macroscopic and microscopic characteristics of the strains were suggestive of three different species, i.e. Microsporum canis, M. gypseum, and M. fulvum. However, partial sequencing of internal transcribed spacer (ITS) region of rDNA, translation elongation factor-1α (Tef-1α), and beta-tubulin (BT2) genes confirmed the identity of both isolates as M. gypseum. The animal was treated with a continuous terbinafine regimen (250 mg/kg) once daily for 12 weeks. Conclusion: To the best of our knowledge, the present report is the first confirmed case of dermatophytosis in a koala. The genetics underlying a variety of phenotypic traits in most classical dermatophyte species are unknown, and further studies are needed to understand this phenomenon.
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- 2016
29. Aspergillus species as emerging causative agents of onychomycosis
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Nouripour-Sisakht, S., Mirhendi, H., Shidfar, M.R., Ahmadi, B., Rezaei-Matehkolaei, A., Geramishoar, M., Zarei, F., and Jalalizand, N.
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- 2015
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30. Morpho-Molecular Characterization of Soil Inhabitant Dermatophytes from Ahvaz, Southwest of Iran, a High Occurrence of Microsporum fulvum
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Rezaei-Matehkolaei, Ali, Jahangiri, Abbas, Mahmoudabadi, Ali Zarei, Najafzadeh, Mohammad Javad, Nouripour-Sisakht, Sadegh, and Makimura, Koichi
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- 2017
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31. Toward a Novel Multilocus Phylogenetic Taxonomy for the Dermatophytes
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de Hoog, G. Sybren, Dukik, Karolina, Monod, Michel, Packeu, Ann, Stubbe, Dirk, Hendrickx, Marijke, Kupsch, Christiane, Stielow, J. Benjamin, Freeke, Joanna, Göker, Markus, Rezaei-Matehkolaei, Ali, Mirhendi, Hossein, and Gräser, Yvonne
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- 2017
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32. In vitro activities of 8 antifungal agents against geophilic dermatophyte isolates
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Jacques Guillot, Ali Rezaei-Matehkolaei, Ali Zarei Mahmoudabadi, Maryam Shariat Nabavi, Forough Shamsizadeh, and Simin Taghipour
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Antifungal Agents ,Itraconazole ,Arthrodermataceae ,Luliconazole ,Broth microdilution ,Imidazoles ,Microbial Sensitivity Tests ,Dermatology ,General Medicine ,Triazoles ,Biology ,medicine.disease_cause ,Griseofulvin ,Microbiology ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,Dermatophyte ,medicine ,Terbinafine ,Efinaconazole ,Geophilic ,medicine.drug - Abstract
BACKGROUND Members of the Nannizzia gypsea complex are globally the most common geophilic dermatophytes which cause infection in animals and human. Although the susceptibility patterns of anthropophilic or zoophilic dermatophyte species to antifungal agents are well documented, the effectiveness of such drugs against geophilic species have rarely been explored. OBJECTIVES This study was aimed to evaluate the in vitro antifungal activity of common and new antifungals against a set of environmental and clinical geophilic dermatophyte isolates. METHODS 108 soil and clinical geophilic isolates from two genera Nannizzia (N. fulva n=59; N. gypsea n=43) and Arthroderma (A. quadrifidum n=4; A. gertleri n=1; A. tuberculatum n=1) were included in the study. The in vitro antifungal susceptibility patterns of eight common and new antifungals against the isolates were determined according to broth microdilution method and by CLSI M38-A3 (3rd edition) protocol. RESULTS MIC values across all isolates from five species ranged as: luliconazole: 0.0002-0.002 µg/mL, terbinafine: 0.008-0.125 µg/mL, efinaconazole: 0.008-0.125 µg/mL, ciclopirox olamine: 0.03-0.5 µg/mL, itraconazole: 0.125-1 µg/mL, amorolfine hydrochloride: 0.125-4 µg/mL, griseofulvin: 0.25-2 µg/mL and tavaborole: 1-8 µg/mL, respectively. CONCLUSION Luliconazole, terbinafine and efinaconazole exhibited the highest in vitro efficacy, regardless of the dermatophyte species. Further surveillance studies are recommended to confirm the implication of such in vitro data for the clinical recovery rate of dermatophytosis with geophilic species following antifungal therapy.
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- 2021
33. Colonization and antifungals susceptibility patterns of Candida species isolated from hospitalized patients in ICUs and NICUs
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Ali Zarei Mahmoudabadi, Ali Rezaei-Matehkolaei, Mojgan Navid, Mehdi Torabizadeh, and Shahnam Mazdarani
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candida species ,amphotericin b ,fluconazole ,terbinafine ,minimum inhibitory concentration ,Pathology ,RB1-214 ,Internal medicine ,RC31-1245 ,Other systems of medicine ,RZ201-999 - Abstract
Background: Several studies have shown that there are an increasing in invasive candidiasis during 2-3 last decades. Although, Candida albicans is considered as the most common candidiasis agents, other non-albicans such as C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis were raised as infectious agents. Resistance to fluconazole among non-albicans species is an important problem for clinicians during therapy and prophylaxis. Objectives: The aim of current study was to detect the Candida species from hospitalized neonatal and children in intensive care units (ICUs) and neonatal intensive care units (NICUs). In addition, the susceptibility of isolated agents were also evaluated against three antifungals. Materials and Methods: In the present study 298 samples including 98 blood samples, 100 urines and 100 swabs from oral cavity were inoculated on CHROMagar Candida. Initial detection was done according to the coloration colonies on CHROMagar Candida. Morphology on cornmeal agar, germ tube formation and growth at 45°C were confirmed isolates. Amphotericin B, fluconazole and terbinafine (Lamisil) were used for the susceptibility tests using microdilution method. Results: In the present study 21% and 34% of urines and swabs from oral cavity were positive for Candida species, respectively. The most common species was C. albicans (62.5%) followed by C. tropicalis (15.6%), C. glabrata (6.3%) and Candida species (15.6%). Our study indicated that the most tested species of Candida, 70.3% were sensitive to fluconazole at the concentration of ≤8 μg/mL. Whereas 9 (14.1%) of isolates were resistant to amphotericine B at ≥8 μg/mL. Conclusions: This study demonstrates the importance of species identification and antifungals susceptibility testing for hospitalized patients in ICUs and NICUs wards.
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- 2015
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34. P009 Investigation of in vitro antifungal susceptibility testing and genetic diversity of clinical isolates of Trichophyton benhamiae and Trichophyton eriotrephon in Iran
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Hasti Kamali Sarvestani, Saham Ansari, Gholam-Reza Shokoohi, Bahram Ahmadi, Ali Rezaei-Matehkolaei, and Mohammad T Hedayati
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Infectious Diseases ,General Medicine - Abstract
Poster session 1, September 21, 2022, 12:30 PM - 1:30 PM Background Trichophyton benhamiae is a zoophilic dermatophyte, known as one of the causative agents of dermatophytosis. Objectives The purpose of this study was to explore the genotypes of T. benhamiae strains isolated from geographically different areas of Iran and also to evaluate in vitro antifungal susceptibility profile of these strains against seven antifungal drugs. Methods A total of 22 strains of T. benhamiae and 2 strains of T. eriotrephon were isolated from patients with distinct types of dermatophytosis. DNA extraction and amplification of rDNA regions using ITS1 and ITS4 primers were conducted on the isolates. The in vitro antifungal susceptibility of posaconazole (PSC), voriconazole (VRC), itraconazole (ITC), ketoconazole (KET), caspofungin (CAS), terbinafine (TRB) and griseofulvin (GRZ) was evaluated according to CLSI M38-A2 protocol. Results The multiple alignments of the ITS-rDNA sequences of T. benhamiae indicated a mean similarity of 99.5%, with 0-3 interspecies nucleotide differences. The geometric mean (GM) values of minimum inhibitory concentrations (MICs) and minimum effective concentrations (MECs) across the all isolates were respectively: TRB: 0.025 mg/l, PSC: 0.032 mg/l, ITC: 0.050 mg/l, and VRC: 0.059 mg/l with lower values and CAS: 0.31 mg/l, KTZ: 0.56 mg/l, and GRZ: 0.76 mg/l with higher values. Conclusion Diverse ITS sequence types of T. benhamiae were shown in different geographical regions of Iran. The TRB, PSC, and ITC were the most effective drugs against T. benhamiae strains, respectively. Furthermore, in our study, two strains of T. eriotrephon as a scarce dermatophyte species were described.
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- 2022
35. S9.4d Main reservoirs of Trichophyton mentagrophytes Type V in Iran
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Sivash Nikkholgh, Ivan Pchelin, Ali Zarei Mahmoudabadi, Mahbubeh Shabanzadeh-Bardar, Maral Gharaghani, Aghil Sharifzadeh, Rasoul Mohammadi, Sadegh Noripour-Sisakht, Farzad Katiraee, and Ali Rezaei-matehkolaei
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Infectious Diseases ,General Medicine - Abstract
S9.4 Skin mycoses and microbiome, September 23, 2022, 4:45 PM - 6:15 PM Objectives Dermatophytosis in livestock receives attention because of its contagiousness, high treatment costs, and lack of control programs. Compared with cattle, mycological aspects of dermatophytosis in sheep and goats have been studied less frequently. Dermatophytosis in these animals (small ruminants) may lead to serious economic losses due to the negative impact on the growth of involved animals, as well as their milk and meat production. Recent studies showed that the old Trichophyton verrucosum var. verrucosum (which is known to have some African and Asiatic sheep as its reservoirs) is currently synonymous with T. mentagrophytes Type V, the most common genotype of T. mentagrophytes isolated from Iranian patients. But the animal reservoirs of this genotype are not well known in Iran and in this investigation, we aimed to determine them. Methods A total of 678 skin and hair samples from animals including sheep (n = 190), cows (n = 79), goats (n = 9), camels (n = 20), stray and domestic cats (n = 195), stray and pet dogs (n = 146), horses (n = 27), foxes (n = 2), hedgehogs (n = 2), and poultries (n = 8) were subjected to direct microscopy and culture on Mycobiotic agar. Most animals had skin lesions, though some stray cats and dogs were asymptomatic. Molecular identification of dermatophyte cultures was done by ITS-rDNA RFLP. To confirm the RFLP identification, 59 representative isolates from all studied animal species were subjected to ITS-rDNA sequencing. The likelihood for isolation of a specific species or genotype with regard to the type of infected animal was determined using the chi-square test. Results We obtained 334 dermatophyte cultures. ITS-RFLP and ITS region sequencing revealed the species T. verrucosum (n = 62; all from cows), T. mentagrophytes Type V (sheep = 95; goat = 6; cat = 1; horse = 2), T. mentagrophytes Type II* (cat = 2), T. mentagrophytes Type VII (dog = 2), Microsporum canis (cats, n = 94; dogs, n = 55; cow, n = 1; horse, n = 1), T. quinckeanum (fox, n = 1), Nannizzia gypsea (cats, n = 5; dogs, n = 4; cow, n = 1; horse, n = 1), and N. fulva (cow, n = 1). No dermatophytes were isolated from camels, hedgehogs, and poultries. There was a statistically significant difference in the isolation rate of T. mentagrophytes Type V between sampled animals meaning that with a high probability it is isolated from sheep and goats. Conclusion Purposive sampling from suspected animals confirmed that sheep are the main animal reservoir of T. mentagrophytes Type V, at least in Iran. Further international sequence-based investigations can test our conclusion.
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- 2022
36. Genotypes analysis of Candida albicans species complex from healthy individual saliva in Ahvaz, Iran
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Kianifar, S., primary, Rezaei-Matehkolaei, A., additional, and Zarei-Mahmoudabadi, A., additional
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- 2022
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37. Time and cost‐efficient identification of Trichophyton indotineae
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Batvandi, Abolfazl, primary, Pchelin, Ivan M., additional, Kiasat, Neda, additional, Kharazi, Mahboobeh, additional, Mohammadi, Rasoul, additional, Zomorodian, Kamiar, additional, and Rezaei‐Matehkolaei, Ali, additional
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- 2022
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38. S9.4d Main reservoirs of Trichophyton mentagrophytes Type V in Iran
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Nikkholgh, Sivash, primary, Pchelin, Ivan, additional, Mahmoudabadi, Ali Zarei, additional, Shabanzadeh-Bardar, Mahbubeh, additional, Gharaghani, Maral, additional, Sharifzadeh, Aghil, additional, Mohammadi, Rasoul, additional, Noripour-Sisakht, Sadegh, additional, Katiraee, Farzad, additional, and Rezaei-matehkolaei, Ali, additional
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- 2022
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39. P009 Investigation of in vitro antifungal susceptibility testing and genetic diversity of clinical isolates of Trichophyton benhamiae and Trichophyton eriotrephon in Iran
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Sarvestani, Hasti Kamali, primary, Ansari, Saham, additional, Shokoohi, Gholam-Reza, additional, Ahmadi, Bahram, additional, Rezaei-Matehkolaei, Ali, additional, and Hedayati, Mohammad T, additional
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- 2022
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40. In vitro antifungal activity of amphotericin B and 11 comparators against Aspergillus terreus species complex
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Vaezi, Afsane, Fakhim, Hamed, Arastehfar, Amir, Shokohi, Tahereh, Hedayati, Mohammad T., Khodavaisy, Sadegh, Rezaei‐Matehkolaei, Ali, Badiee, Parisa, Hagen, Ferry, Lass‐Flörl, Cornelia, Dannaoui, Eric, Meis, Jacques F., and Badali, Hamid
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- 2018
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41. Molecular identification and antifungal susceptibility profiles of Candida dubliniensis and Candida africana isolated from vulvovaginal candidiasis: A single‐centre experience in Iran
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Faeze Maryami, Saham Ansari, Gholam Reza Shokoohi, Ali Rezaei-Matehkolaei, Orazio Romeo, Hossein Mirhendi, Javad Javidnia, Athar Rasekh Jahromi, and Sahand Goodarzi
- Subjects
Adult ,0301 basic medicine ,Antifungal Agents ,Luliconazole ,030106 microbiology ,Candida africana ,Candida dubliniensis ,efinaconazole ,HWP1 gene ,luliconazole ,vulvovaginal candidiasis ,Microbial Sensitivity Tests ,Dermatology ,Iran ,Microbiology ,Fungal Proteins ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Prevalence ,medicine ,Humans ,DNA, Fungal ,Candida albicans ,Efinaconazole ,Candidiasis, Vulvovaginal ,Candida ,Membrane Glycoproteins ,biology ,Imidazoles ,Original Articles ,General Medicine ,Middle Aged ,Triazoles ,biology.organism_classification ,Corpus albicans ,Yeast ,Infectious Diseases ,Nystatin ,chemistry ,Terbinafine ,Female ,Original Article ,medicine.drug - Abstract
Background Vulvovaginal candidiasis (VVC) is a common and debilitating long-term illness affecting million women worldwide. This disease is caused mainly by Candida albicans and a lesser extent by other species, including the two phylogenetically closely related pathogens Candida africana and Candida dubliniensis. Objectives In this study, we report detailed molecular epidemiological data about the occurrence of these two pathogenic yeasts in Iranian patients affected by VVC, or its chronic recurrent form (RVVC), and provide, for the first time, data on the antifungal activity of two new drugs, efinaconazole (EFN) and luliconazole (LUL). Methods A total of 133 vaginal yeast isolates, presumptively identified as C. albicans by phenotypic and restriction analysis of rDNA, were further analyzed by using a specific molecular method targeting the HWP1 gene. All C. africana and C. dubliniensis isolates were also tested for their in-vitro susceptibility to a panel of modern and classical antifungal drugs. Results and conclusions Based on the molecular results, among 133 germ-tube positive isolates, we identify 119 C. albicans (89.47%), 11 C. africana (8.27%), and 3 C. dubliniensis (2.26%) isolates. C. africana and C. dubliniensis showed low MIC values for most of the antifungal drugs tested, especially for EFN and LUL, which exhibited a remarkable antifungal activity. High MIC values were observed only for nystatin and terbinafine. Although C. albicans remains the most common Candida species recovered from Iranian VVC/RVVC patients, our data show that its prevalence may be slightly overestimated due to the presence of difficult-to-identify closely related yeast, especially C. africana.
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- 2021
42. Sheep serve as a reservoir of Trichophyton mentagrophytes genotype V infection.
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Nikkholgh, Sivash, Pchelin, Ivan M, Zarei Mahmoudabadi, Ali, Shabanzadeh-Bardar, Mahbubeh, Gharaghani, Maral, Sharifzadeh, Aghil, Mokhtari Hooyeh, Mohammadreza, Mohammadi, Rasoul, Nouripour-Sisakht, Sadegh, Katiraee, Farzad, and Rezaei-Matehkolaei, Ali
- Abstract
Human infections by Trichophyton mentagrophytes occur mainly due to contact with diseased animals. In Iran, T. mentagrophytes genotype V is the most prevalent variant of the fungus. We aimed to determine the animal reservoir of T. mentagrophytes genotype V infection. The study was done on a total of 577 dermatophyte strains obtained from animals with signs of dermatophytosis and human patients. The list of extensively sampled animals included sheep, cows, cats and dogs. For human cases, epidemiological data were collected. All dermatophyte isolates from animals along with 70 human isolates morphologically similar to T. verrucosum and T. mentagrophytes genotype V were identified by rDNA internal transcribed spacer region restriction fragment length polymorphism analysis and DNA sequencing. A total of 334 animal dermatophyte strains were identified as Microsporum canis, T. mentagrophytes genotype V, T. verrucosum, Nannizzia gypsea, T. mentagrophytes genotype II*, T. mentagrophytes genotype VII, T. quinckeanum , and N. fulva. All clinical isolates identified as T. mentagrophytes genotype V originated from skin and scalp infections. Almost all veterinary isolates of T. mentagrophytes genotype V were cultured from sheep, but epidemiological data on animal-to-human transmission of T. mentagrophytes genotype V infection were limited and we found evidence in favor of interhuman transmission. In Iran, sheep maintain T. mentagrophytes genotype V population and therefore serve as animal reservoir of respective infections. The role of sheep as the source of human dermatophytosis due to T. mentagrophytes genotype V isolates is yet to be proven. [ABSTRACT FROM AUTHOR]
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- 2023
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43. Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species
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Ali Rezaei-Matehkolaei, Shokoofe Shafiei, and Ali Zarei Mahmoudabadi
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Caspofungin ,Fluconazole ,Clotrimazole ,Candida vaginitis ,Candida albicans ,Microbiology ,QR1-502 - Abstract
Background and Objectives: Vulvovaginal candidiasis is a common fungal infection among women during reproductive ages. Although, Candida albicans is accounted as the main etiologic agent of vaginitis, non-albicans species have arisen during last years. Resistant to antifungal drugs especially, fluconazole has been more reported by researchers from around the World. The aims of this study were to determine the prevalence of vulvovaginal candidiasis among suspected patients with vaginitis, the frequency of Candida species, and the susceptibility profiles of isolates to caspofungin, fluconazole and clotrimazole. Materials and Methods: One hundred and twenty suspected women with vaginitis were examined by specialist physician and sampled using moisture swabs. Swabs were inoculated on CHROMagar Candida plates, incubated at 35ºC and detected all isolated Candida species using morphological, microcopy and molecular methods. The antifungal susceptibility tests with caspofungin, fluconazole and clotrimazole were applied using microdilution and Resazurin dye methods against all isolated yeasts. Results: The cultures were positive for 34(28.3%) samples and three Candida species including; C. albicans (88.2%), C. glabrata (8.8%) and C. kefyr (2.9%). Our study shows that only one isolate of C. albicans was resistant to caspofungin at the concentration of 2 μg/ml after 24h incubation that increased to 2 isolates after 48h incubation. All isolates were sensitive to fluconazole at the MIC ranges of 1-0.25 μg/ml, while 88.2% of them were inhibited at 0.25 μg/mL of clotrimazole. Candida albicans remains the most common agent of fungal vaginitis. Conclusion: Although all of Candida isolates were susceptible to fluconazole in vitro, it should be used with caution for empirical therapy due to more resistant rates in clinic. In addition, due to valuable sensitivity of all tested strains to caspofungin, it potentially can be presented as the first line therapy for Candida vaginitis.
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- 2017
44. Genotypes analysis of Candida albicans species complex from healthy individual saliva in Ahvaz, Iran
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S. Kianifar, A. Rezaei-Matehkolaei, and A. Zarei-Mahmoudabadi
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Genotype ,Candida albicans ,Humans ,Iran ,DNA, Fungal ,Saliva ,Applied Microbiology and Biotechnology ,DNA, Ribosomal ,Candida - Abstract
Although Candida albicans is a part of the mycoflora of healthy individuals, it is causing mild to severe forms of candidiasis in patients with underlying diseases. The recent molecular investigations were classified three genotypes, A, B and C for C. albicans. The aim of the present study was to detect different genotypes of C. albicans complex species in a normal population in Iran. Saliva was randomly collected from a normal population, homogenized and cultured on CHROMagar Candida. Classical and molecular methods were used for the detection of isolates. Candida 25S rDNA gene was amplified using the primer pairs of CA-INT-L and CA-INT-R for ABC genotyping of C. albicans. Candida albicans complex was recovered from 103/194 (53·1%) normal papulation. Genotype A with a frequency of 41·7% was the most common isolate, followed by genotype C (34%), genotype B (20·4%) and genotype D (3·9%). Genotyping of C. albicans species complex from healthy individuals showed the presence of three A, B and C genotypes of C. albicans and one D genotype of C. dubliniensis.
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- 2022
45. Quantitative analysis of in vitro biofilm formation by clinical isolates of dermatophyte and antibiofilm activity of common antifungal drugs
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Somayeh Yazdanpanah, Forozan Sasanipoor, Hossein Khodadadi, Ali Rezaei‐Matehkolaei, Farideh Jowkar, Kamiar Zomorodian, Mahboobeh Kharazi, Tooba Mohammadi, Sadegh Nouripour‐Sisakht, Reza Nasr, and Marjan Motamedi
- Subjects
Dermatology - Abstract
The ability of dermatophytes to develop biofilm, as one of the virulence factors in fungal infections which contribute to antifungal resistance, is an outstanding aspect of dermatophytosis that has been noted recently. Because of the paucity of data about the biofilm formation by dermatophytes and their susceptibility to antifungal drugs, this study evaluated the biofilm formation by clinical isolates of dermatophytes and antibiofilm activity of common antifungals widely used to manage dermatophytosis.The ribosomal DNA internal transcribed spacer (ITS) regions sequencing for species identification of 50 clinical dermatophyte isolates was performed. The ability of isolates to form biofilm and inhibitory activity of itraconazole, terbinafine, and griseofulvin against biofilm formation was assayed by the crystal violet staining method. Optical microscopy and scanning electron microscopy (SEM) were applied for the visualization of the biofilm structures.Trichophyton (T.) mentagrophytes (n: 14; 28%) and T. rubrum (n: 13;26%) were included in more than half of the dermatophyte isolates. Biofilm formation was observed in 37 out of 50 (74%) isolates that were classified as follows: nonproducers (n: 13; 26%), weak producers (n: 4; 8%), moderate producers (n: 16; 32%), and strong producers (n: 17; 34%) by comparison of the absorbance of biofilms produced by clinical strains with control. The mean IC50 values for terbinafine, griseofulvin, and itraconazole were 2.42, 3.18, and 3.78 μg/ml, respectively.The results demonstrated that most of the clinical dermatophyte isolates are capable to form biofilm in vitro with variable strength. Moreover, terbinafine can be suggested as the first-line choice for the treatment of biofilm-formed dermatophytosis.
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- 2022
46. Epidemiological Aspects of Dermatophytosis in Khuzestan, southwestern Iran, an Update
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Rezaei-Matehkolaei, Ali, Rafiei, Abdollah, Makimura, Koichi, Gräser, Yvonne, Gharghani, Maral, and Sadeghi-Nejad, Batool
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- 2016
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47. A 9-Month-Old Girl from Iran with Extensive Erythematous Plaques Due to Trichophyton simii, a Zoophilic Dermatophyte
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Ansari, Saham, Hedayati, Mohammad T., Nouripour-Sisakht, Sadegh, Rezaei-Matehkolaei, Ali, Jannesar, Ramin, Mohammadi, Haniyeh, Fazli, Yousef, Ilkit, Macit, and Seyedmousavi, Seyedmojtaba
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- 2016
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48. Dermatophytosis due to Microsporum incurvatum: Notification and Identification of a Neglected Pathogenic Species
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Rezaei-Matehkolaei, Ali, Makimura, Koichi, Graser, Yvonne, Seyedmousavi, Seyedmojtaba, Abastabar, Mahdi, Rafiei, Abdollah, Zhan, Ping, Ronagh, Ali, and Jafarpour, Sima
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- 2016
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49. In vitro antifungal susceptibility patterns of Trichophyton benhamiae complex isolates from diverse origin
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Vit Hubka, Ali Zarei Mahmoudabadi, Jacques Guillot, Hossein Zarrinfar, Adéla Čmoková, Tooba Mohammadi, Forough Shamsizadeh, Kambiz Diba, Ali Rezaei-Matehkolaei, Sadegh Nouripour-Sisakht, Abdollah Rafiei, Kamiar Zomorodian, and Saham Ansari
- Subjects
0301 basic medicine ,Antifungal Agents ,Itraconazole ,Luliconazole ,030106 microbiology ,Dermatology ,Iran ,Biology ,medicine.disease_cause ,Microbiology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Japan ,Tinea ,Zoonoses ,medicine ,Animals ,Humans ,Efinaconazole ,Ciclopirox Olamine ,Tavaborole ,Arthrodermataceae ,General Medicine ,Griseofulvin ,United States ,Europe ,Infectious Diseases ,chemistry ,Dermatophyte ,Terbinafine ,medicine.drug - Abstract
Background Species from the Trichophyton benhamiae complex are mostly zoophilic dermatophytes which cause inflammatory dermatophytosis in animals and humans worldwide. Objectives This study was purposed to (a) to identify 169 reference and clinical dermatophyte strains from the T benhamiae complex species by molecular method and adhering to the newest taxonomy in the complex (b) to evaluate the in vitro antifungal susceptibility profile of these strains against eight common and new antifungal agents that may be used for the treatment of dermatophytosis. Methods All isolates, mainly originated from Europe but also from Iran, Japan and USA, were subjected to ITS-rDNA sequencing. The in vitro antifungal susceptibility profiles of eight common and new antifungal drugs against the isolates were determined by CLSI M38-A2 protocol and according to microdilution method. Results Based on the ITS-rDNA sequencing, T benhamiae was the dominant species (n = 102), followed by T europaeum (n = 29), T erinacei (n = 23), T japonicum (n = 10), Trichophyton sp (n = 4) and T eriotrephon (n = 1). MIC ranges across all isolates were as follows: luliconazole: 0.0002-0.002 µg/ml, terbinafine: 0.008-0.125 µg/ml, efinaconazole: 0.008-0.125 µg/ml, ciclopirox olamine: 0.03-0.5 µg/ml, itraconazole: 0.06-2 µg/ml, griseofulvin: 0.25-4 µg/ml, amorolfine hydrochloride: 0.125-4 µg/ml and tavaborole: 1-16 µg/ml. Conclusion Luliconazole, efinaconazole and terbinafine were the most potent antifungals against T benhamiae complex isolates, regardless of the geographic locations where strains were isolated. These data might help dermatologists to develop effective therapies for successful treatment of infections due to T benhamiae complex species.
- Published
- 2021
50. Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized paediatric patients with urinary tract infection in Iran
- Author
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Maral Gharaghani, A Zarei Mahmoudabadi, Amir Kamal Hardani, and Ali Rezaei-Matehkolaei
- Subjects
Posaconazole ,Antifungal Agents ,Genotype ,Microbial Sensitivity Tests ,Iran ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,Drug Resistance, Fungal ,Candida albicans ,medicine ,Humans ,Typing ,Child ,Fluconazole ,Genotyping ,Candida ,030304 developmental biology ,0303 health sciences ,Genetic diversity ,biology ,030306 microbiology ,General Medicine ,biology.organism_classification ,Corpus albicans ,Urinary Tract Infections ,Biotechnology ,medicine.drug - Abstract
Aims The present study aimed to determine the microsatellite length polymorphism (MLP) genotypic patterns and antifungal susceptibility profiles of Candida albicans isolated from patients with candiduria. Methods and results DNA of 50 C. albicans isolates was used for molecular identification based on the ITS1 -5.8s-ITS2 region. MLP assays were performed to amplify three loci (EF3, CDC3 and HIS3), and PCR products were used for fragment analysis. Antifungal susceptibility tests were performed according to CLSI M27 4th ed guidelines. In all, 38 different genotypes were detected with the three polymorphic loci among C. albicans isolates, and only one genotype was homozygous. In comparison to other countries, our genotypes were divided into three clusters, two of which were linked to France and a third of which was linked to Austria. The genetic structures of the 50 C. albicans isolates revealed varied heterozygosity and significant Hardy-Weinberg equilibrium at the EF3 locus. Only one (2%) and four (8%) of isolates showed resistance to fluconazole and itraconazole, respectively. In C. albicans genotype G25, one (2%) of the isolates showed cross-resistance and non-wild-type resistance to posaconazole, itraconazole and fluconazole. Conclusion MLP typing is a useful tool to analyse the genetic structure of C. albicans isolates. High genetic diversity (38 genotypes) was detected in the isolates tested here. Compared to isolates in other countries, the ones from our patients had a clear relationship with French and Austrian isolates. Significance and impact of the study Iranian isolates of C. albicans have a distinct genotype and show similarities only with French and Austrian isolates.
- Published
- 2021
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