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Background: Thrombosis is closely related with cancer patients. Venous thromboembolism such as pulmonary thromboembolism and deep vein thrombosis are more frequently occurred in cancer patients. Arterial thromboembolism such as myocardial infarction and ischemic stroke were less occurred. Venous thromboembolism and arterial thromboembolism were rarely concomitantly occurred in cancer patients, especially ahead of cancer diagnosis. Methods: Five patients who suffered from venous thromboembolism and arterial thromboembolism sequentially and then cancer was diagnosed were enrolled and followed up until the patient died or until June 2021. The medical records were retrospectively reviewed to evaluate the clinical characteristics, image findings and the outcomes.Results: Three women and two men were renrolled, with a mean age of 63.2 years (range, 47-76 years). Four patients suffered from ischemic stroke, and the lesions showed by magnetic resonance manifested bilateral diffuse changes. One patient suffered from lower limb arterial thromboembolism. Four patients suffered from pulmonary thromboembolism diagnosed by CTPA or ultrasound (among them, two patients with deep venous thrombosis simultaneously), one patient suffered from solely deep venous thrombosis. Pulmonary adenocarcinoma was diagnosed in two patients, and one skin basal cell carcinoma, one ovarian clear cell adenocarcinoma and one rectal adenocarcinoma were diagnosed in the other three patients. Four patients received cancer related treatment and all patients received anticoagulation therapy. Except the patient without cancer related treatment who survived for 3 months, all the other four patients survived more than 2 years.Conclusion: Cancer can present as venous thromboembolism or arterial thromboembolism or both in advance. Cancer should be screened in patients with venous thromboembolism, especially those with concomitant arterial thromboembolism.

This study aimed to investigate the association between maternal insulin resistance (IR) in the late second trimester and pregnancy outcomes, as well as to identify risk factors of IR among women with gestational diabetes mellitus (GDM).A retrospective study was conducted among 2647 women diagnosed with GDM. IR was evaluated using the homeostasis model assessment method for IR (HOMA-IR) in the late second trimester (between 24 and 28 weeks), and the lipid profiles were measured at the same time. Patients were divided into groups based on quartiles of HOMA-IR. The information on pregnancy outcomes and risk factors was extracted from the medical records of all participants and entered electronically. Logistic regression models were used to analyze the associations between HOMA-IR and pregnancy outcomes, as well as the associations between risk factors and HOMA-IR.Greater IR was associated with cesarean delivery, preterm delivery, macrosomia, and large for gestational age newborns, but only significantly associated with preterm delivery after adjustment for potential confounders (P .001). Prepregnancy body mass index (BMI), weight gain before diagnosis of GDM, and triglycerides were significantly related with IR in the late second trimester (P .001). Besides, the total area under the curve of the diagnostic 75-g oral glucose tolerance test and glycosylated hemoglobin A1c increased from the lowest to the highest HOMA-IR groups.Increasing IR in the late second trimester predicts adverse pregnancy outcomes especially for preterm delivery in women with GDM. Additionally, prepregnancy BMI and weight gain before diagnosis of GDM are independent risk factors for the development of IR.背景: 本研究旨在探讨妊娠期糖尿病(gestational diabetes mellitus, GDM)患者中孕期后期的胰岛素抵抗水平(insulin resistance, IR)与其妊娠结局的关系, 及GDM患者IR相关的危险因素。 方法: 本研究为回顾性研究, 共纳入2647名GDM患者。回顾临床资料, 采用妊娠24至28周指标计算稳态模型评估IR水平(homeostasis model assessment insulin resistance, HOMA-IR)以评价患者中孕期后期IR水平, 并于同期检测患者血脂水平。根据HOMA-IR的四分位值将患者分为四组。患者的妊娠结局及相关危险因素由患者病历提取并电子录入。Logistics回归模型被用于分析HOMA-IR与妊娠结局的关系, 及各危险因素与HOMA-IR的关系。 结果: 更高水平的IR与剖宫产分娩、早产、巨大儿及大于胎龄儿相关, 但经混杂因素校正后仅与早产结局显著相关(P.001)。孕前体重指数(body mass index, BMI)、GDM诊断前孕期体重增长及甘油三酯水平与中孕期后期的IR水平显著相关(P.001)。此外, 75克口服糖耐量诊断试验曲线下面积与糖化血红蛋白A1c水平在HOMA-IR四分组中逐渐升高。 结论: 中孕期后期IR水平增加可预测GDM患者不良妊娠结局特别是早产的发生。另外, 孕前BMI及GDM诊断前孕期体重增长是中孕期后期高IR水平的独立危险因素。.

In this study,mouse models of benign prostatic hyperplasia induced by subcutaneous injection of testosterone propionate was used to investigate the therapeutic effect and mechanism of Urtica hyperborean( UW) extracts on prostate hyperplasia in mice. The effects of UW extracts on prostate index,serum epidermal growth factor( EGF) and dihydrotestosterone( DHT) in model mice were observed,and the EGF and anti-apoptotic factor( Bcl-2) mRNA expression levels were detected as well as pathological changes in prostate tissue. The results showed that the ethyl acetate extraction and alcohol soluble fraction of the UW could significantly reduce the prostate index,reduce the serum DHT and EGF levels( P0. 01),and significantly decrease the EGF and Bcl-2 mRNA expression( P0. 01),significantly improved the morphological structure of prostate tissue. The above results confirmed that ethyl acetate extract and alcohol-soluble parts of UW have a good preventive effect on mice prostatic hyperplasia model,and its mechanism may be to reduce androgen levels by regulating polypeptide growth factors and/or inhibiting cell hyperproliferation and promoting apoptosis. This study laid the foundation for the further research on UW.

This paper deals with the application of ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS/MS) method to rapidly determine and analyze the chemical constituents of methanol extract of Urtica hyperborea. We employed UPLC YMC-Triart C18(2. 1 mm×100 mm,1. 9 μm) column to UPLC analysis with acetonitrile-water(containing 0. 4% formic acid) in gradient as mobile phase. The flow rate was 0. 3 m L·min-1 gradient elution and column temperature was 30℃; the injection volume was 4 μL. ESI ion source was used to ensure the data collected in anegative ion mode. The chemical components of U. hyperborea were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data.The results indicated that a total of 31 compounds were identified,including 8 flavonoids,14 phenolic compounds,8 phenylpropanoids(4 coumarins and 4 lignans),and 1 steroidal compound,13 of which were confirmed by comparison. The UPLC-ESI-Q-TOF-MS/MS method could rapid identify the chemical components of U. hyperborea. The above compounds were discovered in U. hyperborea for the first time,which could provide theoretical foundation for further research on the basis of the pharmacodynamics of U. hyperborea.

In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb. by DEAE sepharose FF and Superdex™ 75 gel chromatography. The average molecular weight (Mw) of EFSP-1 was 8.75 kDa. Monosaccharides composition analysis indicated that EFSP-1 was a glucan. The structure of EFSP-1 was characterized by analysis of FT-IR, GC–MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp. Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells. The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells. Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.

These sausages were manufactured with mutton and tail fat by starter culture. The microbiological of fermented mutton sausages were evaluated to determine their quality characteristics during ripening and storage. Two groups of fermented sausages manufacture using the same technology were named control group (CO) and starter culture group (SC). In this study, Lactic acid bacteria counts in sausages made with combinations of starter cultures increased to 8 logcfu/g. The total bacteria counts in the SC were lower (P < 0.05) than in the control (CO) after drying and storage time. During ripening, total bacterial counts in the SC were 7logcfu/g, which was significantly lower (p

A kind of 3-RPS and 3-DOF parallel robotic mechanisms is used as motion-sensible-platform of gun training simulator to implement the motion simulation. Its dynamics is analyzed and driving force of joint is gained. This paper introduces the study on motion generation of gun training simulator. The moving model of gun is established on the basis of the model of vehicle. We solve the pose of gun by applying the theory of homogeneous matrix. In order to ensure actuators moving at a preset speed and enable the motion-sensible-platform to perform a real-time moving posture simulation of a wheeled gun vehicle in running, the speed equation of actuator is given. The feasibility of models was tested through the simulation experiments. All of these works are beneficial to optimize design and control realization of motion platform structure.

Based on standard k-ε turbulent, PISO algorithm and the dynamic mesh model, numerical simulation of the 3D, viscous, unsteady compressible gas synthetic jet model was proposed. Two kinds of diaphragm boundary conditions， Helmholtz frequency characteristics and other factors affecting the performance of the 3D synthetic jet were discussed. Simulation results show that the dynamic mesh diaphragm boundary condition is more consistent with the experimental results and the theoretical results, which demonstrates the rationality of the dynamic mesh 3D compressible gas model.

The engineering graph recognition course studies on theory and methods of engineering graph systematically. The necessity to set up engineering graph recognition course in computer science and technology major and the teaching method based on creative thoughts and application ability training is discussed in this paper in order to developing new path and provides new way of thinking for the educational reform pilot project reform.

The title eremophilenolide, C(15)H(22)O(3), is a natural compound isolated from Senecio laetus Edgew. The two cis-fused six-membered rings have chair confomations and the five-membered ring has a planar envelope conformation [maximum deviation = 0.010 (1) Å]. The β-hy-droxy group participates in inter-molecular O-H⋯O hydrogen bonding, forming mol-ecular chains along the a axis.

To explore mitochondrial DNA (mtDNA) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide (CTAB) method.Thirteen Lucilia sericata (Meigen) and 13 Nicrophorus fossor (Erichson) were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district. Four parts (head, chest muscle, legs and wings) of insect were collected, and the mtDNA of all samples were extracted using CTAB method. The purity and concentration were tested using protein and nucleic acid spectrophotometry. The integrity of the extracted mtDNA and PCR products were checked by agarose gel electrophoresis. The PCR products were sequenced and the obtained sequences were imputed into GenBank for comparison.mtDNA were successfully extracted from 10 head samples, 6 legs samples, 4 wing samples and 13 chest muscle samples of the Lucilia sericata (Meigen). Also, mtDNA were successfully extracted from 5 head samples, 8 legs samples, 3 wing samples and 13 chest muscle samples of the Nicrophorus fossor (Erichson).mtDNA can be obtained from chest muscle and other parts of sarcosaphagous insects using the improved CTAB method.

Supplemental Digital Content is available in the text, Our previous study indicated overexpression of metadherin (MTDH) is an adverse prognostic factor in squamous cell carcinoma of the head and neck (SCCHN) and promotes SCCHN cell proliferation and invasion. However, its mechanism remains unclear. Recent studies have indicated that MTDH is a cancer-metastasis-associated molecule that participates in the process of angiogenesis. Therefore, the study is aimed to investigate that whether vascular endothelial growth factor (VEGF), as one of the most potent proangiogenic cytokines, is regulated by MTDH and the role of the phosphatidylinositide 3-kinases/Protein Kinase B (PI3K/Akt) pathway in this process of regulation and the clinical significance of both MTDH and VEGF in SCCHN. Immunohistochemistry was used to assay the expression of MTDH and VEGF in a cohort of 189 SCCHN patients with intact follow-up information. The expression of MTDH was then upregulated or inhibited by lentivirus-mediated MTDH Complementary deoxyribonucleic acid or MTDH short hairpin ribonucleic acid (shRNA) to observe the resulting alterations in VEGF expression and the PI3K/Akt signaling pathway in SCCHN cell lines. In addition, the PI3K/Akt pathway was modulated to observe the resulting changes in the MTDH-mediated expression of VEGF. The immunohistochemistry data showed that MTDH expression is positively correlated with VEGF expression in SCCHN tissues. Moreover, the overexpression of MTDH in SCCHN Tu686 and 5-8F cells led to increases in the expression of VEGF, and this effect was accompanied by activation of the PI3K/Akt pathway. Conversely, shRNA-mediated knockdown of MTDH led to decreased VEGF expression. In addition, inhibition of the Akt signaling pathway reversed the upregulation of VEGF resulting from MTDH overexpression. Moreover, the survival analysis revealed that VEGF is an independent prognostic factor, and a combined survival analysis based on both MTDH and VEGF showed synergistic effects in the prognosis evaluation of SCCHN patients. The findings of the present study demonstrate that MTDH regulates the expression of VEGF via the PI3K/Akt signaling pathway, indicating the potential role of the MTDH-mediated activation of VEGF signaling pathway in SCCHN angiogenesis and metastasis.