9 results on '"Ribeiro, Karla Veloso Gonçalves"'
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2. Protective immunity triggered by ectonucleoside triphosphate diphosphohydrolase-based biopharmaceuticals attenuates cardiac parasitism and prevents mortality in Trypanosoma cruzi infection
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Paula, Alessandra Teixeira, Ribeiro, Karla Veloso Gonçalves, Cardoso, Kimberly Freitas, Bastos, Daniel Silva Sena, Santos, Eliziária Cardoso, Novaes, Rômulo Dias, Cardoso, Silvia Almeida, and Oliveira, Leandro Licursi
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- 2022
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3. The regeneration conferring transcription factor complex ERF115‐PAT1 coordinates a wound‐induced response in root‐knot nematode induced galls
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Ribeiro, Cleberson, primary, de Melo, Bruno Paes, additional, Lourenço‐Tessutti, Isabela Tristan, additional, Ballesteros, Helkin Forero, additional, Ribeiro, Karla Veloso Gonçalves, additional, Menuet, Killian, additional, Heyman, Jefri, additional, Hemerly, Adriana, additional, de Sá, Maria Fatima Grossi, additional, De Veylder, Lieven, additional, and de Almeida Engler, Janice, additional
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- 2023
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4. The regeneration conferring transcription factor complex ERF115‐PAT1 coordinates a wound‐induced response in root‐knot nematode induced galls.
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Ribeiro, Cleberson, de Melo, Bruno Paes, Lourenço‐Tessutti, Isabela Tristan, Ballesteros, Helkin Forero, Ribeiro, Karla Veloso Gonçalves, Menuet, Killian, Heyman, Jefri, Hemerly, Adriana, de Sá, Maria Fatima Grossi, De Veylder, Lieven, and de Almeida Engler, Janice
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ROOT-knot nematodes ,TRANSCRIPTION factors ,ROOT-knot ,REGENERATION (Biology) ,WOUND healing ,GALLS (Botany) - Abstract
Summary: The establishment of root‐knot nematode (RKN; Meloidogyne spp.) induced galls in the plant host roots likely involves a wound‐induced regeneration response. Confocal imaging demonstrates physical stress or injury caused by RKN infection during parasitism in the model host Arabidopsis thaliana.The ERF115‐PAT1 heterodimeric transcription factor complex plays a recognized role in wound‐induced regeneration. ERF115 and PAT1 expression flanks injured gall cells likely driving mechanisms of wound healing, implying a local reactivation of cell division which is also hypothetically involved in gall genesis.Herein, functional investigation revealed that ectopic ERF115 expression resulted in premature induction of galls, and callus formation adjacent to the expanding female RKN was seen upon PAT1 upregulation. Smaller galls and less reproduction were observed in ERF115 and PAT1 knockouts. Investigation of components in the ERF115 network upon overexpression and knockdown by qRT‐PCR suggests it contributes to steer gall wound‐sensing and subsequent competence for tissue regeneration. High expression of CYCD6;1 was detected in galls, and WIND1 overexpression resulted in similar ERF115OE gall phenotypes, also showing faster gall induction.Along these lines, we show that the ERF115‐PAT1 complex likely coordinates stress signalling with tissue healing, keeping the gall functional until maturation and nematode reproduction. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Effects of aluminum on the external morphology of root tips in rice
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Brito, Danielle Santos, primary, Neri-Silva, Roberto, additional, Ribeiro, Karla Veloso Gonçalves, additional, Peixoto, Paulo Henrique Pereira, additional, and Ribeiro, Cleberson, additional
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- 2020
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6. Evaluation of the immunogenic and protective potential of two vaccine strategies against Chagas disease: DNA enconding NTPDase-1 and recombinant NTPDase-1 in BALB/c female mice
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Ribeiro, Karla Veloso Gonçalves, Cardoso, Sílvia Almeida, Santos, Eliziária Cardoso dos, and Oliveira, Leandro Licursi de
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Imunologia ,Trypanosoma cruzi ,Chagas, Doença de - Vacina ,DNA recombinante ,Ciências Biológicas - Abstract
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior A doença de Chagas é causada pelo protozoário hemoflagelado Trypanosoma cruzi. Hospedeiros mamíferos são normalmente infectados através do contato de fezes contaminadas do vetor triatomíneo com feridas na pele, conjuntiva e membranas mucosas oral e nasal. O Benznidazol é o único medicamento para tratamento da doença no Brasil, entretanto apresenta diversos efeitos colaterais e é eficiente apenas na fase aguda. Embora a transmissão vetorial seja o mecanismo predominante, a transmissão oral tornou-se comum no Brasil. Tendo em vista o crescimento do número de casos não vetoriais de transmissão da doença, além do tardio ou ausente diagnóstico e toxicidade do medicamento disponível, é de extrema importância desenvolver uma vacina eficaz e segura para controle da doença. Assim, o objetivo do trabalho foi avaliar a resposta imune induzida pela vacina de DNA recombinante codificando NTPDase-1 de Trypanosoma cruzi (DNA/NTPDase-1) e pela vacina de NTPDase-1 recombinante (rNTPDase-1) em camundongos BALB/c. A imunização com a proteína recombinante induziu produção mais rápida de IgG total específica e níveis mais elevados de IgG1 e IgG2a em comparação a vacina de DNA/NTPDase-1. O perfil de células T revelou frequência similar de CD3 + /CD4 + e CD3 + /CD8 + entre os grupos proteína, DNA e controle. Houve aumento na frequência de células T ativadas (CD4 + /CD44 + e CD8 + /CD44 + ) no grupo vacinado com rNTPDase-1 em comparação aos grupos controle e DNA/NTPDase-1. O nível de citocinas Th1 (TNF-α e IFN-γ) não diferiu entre os grupos vacinados, enquanto o nível de citocinas Th2 (IL-6 e IL-10) foi menor nos grupos DNA e proteína recombinante em relação ao controle. O nível de óxido nítrico (NO) aumentou em camundongos imunizados com a proteína recombinante. Após o desafio, observamos que a imunização com DNA/NTPDase-1 reduziu a carga parasitária no tecido cardíaco quando comparada aos grupos controle e rNTPDase-1. Além disso, observamos sobrevivência de 100% nos dois grupos vacinados, enquanto 50% dos animais morreram no grupo controle. Dessa forma, o estímulo para produção de anticorpos específicos, a ativação de células T CD4 + e CD8 + , a redução na produção de citocinas anti-inflamatórias e o aumento da produção de NO obtidos no esquema vacinal usando proteína rNTPDase-1 e a redução da carga parasitária no tecido cardíaco induzido por DNA/NTPDase-1 demonstram a potencialidade desse antígeno para o desenvolvimento de vacina para o controle da doença de Chagas. Chagas disease is caused by haemoflagellated protozoan Trypanosoma cruzi. Mammal hosts are ordinarily infected through of contact of the triatomine vector-contaminated feces with skin breaks, conjunctiva, oral and nasal mucosa. Benznidazole is the only drug to treat the disease in Brazil however it has several side effects and is effective only in the acute phase. Although vector transmission is the predominant mechanism, oral transmission has become common in Brazil. In view of the increase in the number of non-vector cases of disease transmission, in addition to the late or absent diagnosis and toxicity of the available drug, it is extremely important to develop an effective and safe vaccine for disease control. Thus, the aim of the work was to evaluate the immune response induced by recombinant DNA vaccine encoding T. cruzi NTPDase-1 (DNA/NTPDase-1) and recombinant NTPDase-1 vaccine in BALB/c mice. The immunization with the recombinant protein induced faster production of total specific IgG and higher levels of IgG1 and IgG2a compared to DNA/NTPDase-1 vaccine. The T cell profile showed a similar frequency of CD3 + /CD4 + and CD3 + /CD8 + between the protein, DNA and control groups. There was an increased frequency of activated T cells (CD4 + /CD44 + e CD8 + /CD44 + ) in rNTPDase-1 vaccinated group compared to the control and DNA/NTPDase-1 groups. The level of Th1 cytokines (TNF-α and IFN-γ) did not differ between the vaccinated groups, while the level of Th2 cytokines (IL-6 and IL-10) was lower in the DNA and recombinant protein groups compared to the control. The nitric oxide (NO) level increased in immunized mice with recombinant protein. After the challenge, we observed immunization with DNA/NTPDase-1 reduced parasite burden in cardiac tissue when compared to the control and rNTPDase-1 groups. Moreover we observed 100% survival on both vaccinated groups, while 50% of the animals died in the control group. Therefore, the stimulus for specific antibody production, the activation of CD4 + and CD8 + T cells, reduction in the production of anti-inflammatory cytokines and increase of NO production in the vaccine scheme using rNTPDasse-1 protein and the reduction of parasitic load in the cardiac tissue induced by DNA/NTPDase-1 demonstrate the potential of this antigen for the development of vaccine for the control of Chagas ́s disease.
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- 2018
7. Bacteriophage Isolated from Sewage Eliminates and Prevents the Establishment of Escherichia Coli Biofilm
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Ribeiro, Karla Veloso Gonçalves, primary, Ribeiro, Cleberson, additional, Dias, Roberto Sousa, additional, Cardoso, Silvia Almeida, additional, de Paula, Sergio Oliveira, additional, Zanuncio, Jose Cola, additional, and Oliveira, Leandro Licursi de, additional
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- 2018
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8. Ecophage 017 acts in the Escherichia coli biofilm prevention and degradation
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Ribeiro, Karla Veloso Gonçalves, Oliveira, Leandro Licursi de, Paula, Sérgio Oliveira de, and Silveira, Wendel Batista da
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Bactéria ,Bacteria ,Biofilm ,CIENCIAS BIOLOGICAS::BIOLOGIA GERAL [CNPQ] ,Biofilme ,Bacteriófago ,Bacteriophage - Abstract
The bacteria are usually found attached to surfaces forming dense microbial communities called biofilms. The formation of biofilms has been documented as a survival strategy of microorganisms conferring resistance to antibiotics, disinfectants, biocides and the host immune system. The aim of this study at investigating the ability of the lytic bacteriophage Ecofago 017 to prevent the biofilm formation by Escherichia coli and degrade established biofilms. The conditions for growth and development of biofilms were evaluated and standardized. Different concentrations of phage were tested to define the optimal to prevention of biofilm formation and degradation. Analysis by Scanning Electron Microscopy and Confocal Microscopy was performed to support these results. We observed that strains of E. coli 30 biofilm have formed in air-liquid interface in the conditions tested, with higher production after 48 h. The experiments showed that virtually all concentrations of bacteriophage Ecophage 017 reduced both bacterial growths, as the amount of biofilm formed. The minimum dose able to significantly reduce the biofilm was 10 2 PFU / mL (Multiplicity of infection, MOI, 10-5), lower than described in literature. Experiments of degradation showed up to 85% reduction in biofilm mass after exposure to the phage, suggesting this is able to significantly reduce established E. coli 30 biofilms, indicating its ability to access and infect bacteria on the inside, causing cell death. Therefore the results show that the Ecophage 017 could be used both to reduce the fixing as to lyse bacterial cells associated with biofilms, generating biotechnological potential applicabilities to this phage. As bactérias são comumente encontradas aderidas a superfícies formando comunidades microbianas densas chamadas biofilmes. A formação do biofilme tem sido documentada como estratégia de sobrevivência dos micro-organismos conferindo resistência a antimicrobianos, desinfetantes, biocidas e ao sistema imune do hospedeiro. O objetivo deste trabalho foi investigar a capacidade do bacteriófago lítico Ecofago 017 em prevenir a formação de biofilme por Escherichia coli e degradar biofilmes já estabelecidos. As condições de crescimento e desenvolvimento de biofilmes foram avaliadas e padronizadas. Diferentes concentrações de fagos foram testadas a fim de definir as melhores para prevenção da formação de biofilme e para degradação de biofilmes estabelecidos. Análises por Microscopia Eletrônica de Varredura e Microscopia Confocal foram realizadas para dar suporte a tais resultados. Observamos que cepas de E. coli 30 formaram biofilme na interface líquido-ar nas condições testadas, com maior produção após 48 h. Os experimentos de prevenção e degradação revelaram que praticamente todas as concentrações do bacteriófago Ecofago 017 reduziram tanto o crescimento bacteriano, quanto a quantidade de biofilme formado. A dose mínima capaz de reduzir significativamente o biofilme foi 102 UFP/mL (Multiplicidade de infecção, MOI, de 10-5), bem inferior a descrita na literatura. Os experimentos de degradação exibiram redução de até 85% na massa do biofilme após exposição ao fago, sugerindo que este é capaz de reduzir significativamente biofilmes já estabelecidos de E. coli 30 e indicando sua habilidade em acessar o biofilme e infectar bactérias em seu interior, causando morte celular. Portanto, os resultados indicam que o Ecofago 017 pode ser usado tanto para reduzir a fixação bacteriana quanto para lisar células associadas a biofilmes de E. coli, gerando potenciais aplicabilidades biotecnológicas para este fago.
- Published
- 2012
9. Degradation of Green Polyethylene by Pleurotus ostreatus
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da Luz, José Maria Rodrigues, primary, Paes, Sirlaine Albino, additional, Ribeiro, Karla Veloso Gonçalves, additional, Mendes, Igor Rodrigues, additional, and Kasuya, Maria Catarina Megumi, additional
- Published
- 2015
- Full Text
- View/download PDF
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