1. Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of A Real-Time PCR Method for Its Detection
- Author
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Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Generalitat Valenciana, Ministério da Educação, Brazil, Agrícola Villena Cooperativa, Ministerio de Agricultura, Pesca, Alimentación y Medio Ambiente, European Commission, Ministerio de Ciencia e Innovación, Ribeiro-Teresani, Gabriela, Bertolini, Edson, Alfaro Fernández, Ana Olvido, Martínez, Carmen, Ossamu Tanaka, Francisco Andre, Kitajima, Elliot W., Rosello, Montserrat, Sanjuan, Susana, Ferrándiz, Juan Carlos, López, María Milagros, Cambra, Mariano, Font San Ambrosio, Maria Isabel, Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Generalitat Valenciana, Ministério da Educação, Brazil, Agrícola Villena Cooperativa, Ministerio de Agricultura, Pesca, Alimentación y Medio Ambiente, European Commission, Ministerio de Ciencia e Innovación, Ribeiro-Teresani, Gabriela, Bertolini, Edson, Alfaro Fernández, Ana Olvido, Martínez, Carmen, Ossamu Tanaka, Francisco Andre, Kitajima, Elliot W., Rosello, Montserrat, Sanjuan, Susana, Ferrándiz, Juan Carlos, López, María Milagros, Cambra, Mariano, and Font San Ambrosio, Maria Isabel
- Abstract
[EN] A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect 'Ca. L. solanacearum'. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of 'Ca. Liberibacter' were observed using electron microscopy in celery plant tissues. A fifth haplotype of 'Ca. L. solanacearum', named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.
- Published
- 2014