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Herbicides are the foundation for row-middle weed control in Florida plasticulture production. Paraquat is commonly used as a burndown herbicide, and resistance issues have subsequently developed. Halosulfuron is mixed with PRE and POST herbicides to provide additional control of nutsedge. The objective of the study was to determine glufosinate efficacy on weeds emerging in the row-middle and suitability in mixture with halosulfuron for nutsedge control. For total weed control, the high dose of glufosinate (983 g ai ha-1) gave the highest overall control (98% and 64% at 4 wk after treatment for experiments 1 and 2, respectively), and the low rate of glufosinate (656 g ha-1) (67% and 39%) gave results comparable to paraquat (57% and 44%). The high glufosinate dose and paraquat gave comparable control of Brazil pusley (74% to 77% control). Glufosinate + halosulfuron mixture had lower efficacy on Brazil pusley than halosulfuron + paraquat mixture. Glufosinate application reduced grass densities, whereas paraquat did not. Increasing the glufosinate dose did not further decrease grass densities. Similar trends in grass control were also demonstrated in their respective mixtures. Mixing halosulfuron with glufosinate or paraquat did not provide consistent reductions in nutsedge densities, nor did adding paraquat or glufosinate further reduce densities compared with halosulfuron alone for the 4-wk study period. Both paraquat and glufosinate antagonized halosulfuron and reduced efficacy on nutsedge. Compared to controls, there was a reduction between expected and actual nutsedge control for paraquat and glufosinate (25% and 36%), respectively. For total weed control, glufosinate is a suitable alternative to paraquat for row-middle weed management in vegetable production.Nomenclature: Glufosinate; halosulfuron; paraquat; Brazil pusley, Richardia brasiliensis Gomes RCHBR; nutsedge, Cyperus spp.

The use of pre-emergent herbicides offers a promising alternative for proper management of difficult-to-control weed species in soybeans, such as white-eye ( Richardia brasiliensis Gomes), a glyphosate-tolerant species, and weeds in the Amaranthus genus (commonly referred to as pigweeds). Here, we aimed at determining whether weed control efficacy and crop selectivity are altered when pre-emergent herbicide applications take place either prior to, or right after crop sowing. To this end, field trials were conducted employing 10 preemergent herbicide treatments (plus an untreated control as well as an untreated, weed-free treatment), replicated four times and sprayed either before, or right after soybean sowing. Results indicate that the actual timing of pre-emergent herbicide spraying relative to soybean sowing significantly changed the weed control efficiency set forth by most herbicide active ingredients tested, with no change to their selectivity to crop plants whatsoever. Some herbicides (e.g. diclosulam, mesotrione, flumioxazin, and a flumioxazin + imazethapyr mixture), however, were equally effective for controlling white-eye and pigweeds regardless of application timing. Moreover, the only herbicide treatments allowing for satisfactory (>70%) control of all target weed species (white-eye, pigweeds, and black oats, Avena strigosa Schreb.) were flumioxazin and a flumioxazin + imazethapyr mixture. Spraying performed after sowing resulted in average yield gains of over 600 kg ha-1 relative to pre-sowing applications, possibly owing to better control of broadleaves. Altogether, this information is useful to soybean producers, as it does not lead to an increase in overall costs; instead, it only affects the timing of entry into the area for pre-emergent herbicide application.

This work aimed to morphologically characterize 22 species of Spermacoceae s.s found in the Atlantic Forest in the state of Rio de Janeiro with the purpose of improving palynological knowledge of the studied species. The studied species included six species of Borreria and Denscantia cymosa, Emmeorhiza umbellata, Hexasepalum apiculatum, H. radula, H. teres, Manettia fimbriata, Mitracarpus eichleri, M. frigidus, M. hirtus, M. lhotzkyanus, M. megapotamicus, Oldenlandia salzmannii, Pentodon pentandrus, Richardia brasiliensis, Richardia scabra and Spermacoce rubescens. Acetolysed pollen grains were analyzed using light (LM) and scanning electron miscroscopy (SEM). The pollen grains varied in size (polar diameter) from medium to large, with the exception of Hexasepalum teres which was very large. Pollen grains were oblate spheroidal in most species, suboblate in six species, prolate spheroidal in Spermacoce rubescens and subprolate in Manettia fimbriata. All pollen grains were colporate, while number of apertures varied (three-19). The endoaperture was lolongate in most species and lalongate in four species. Sexine ornamentation varied among reticulate, reticulate-granulate, microreticulate, granulate with perforation, echinate-perforate and vermiculate. Pollen attributes were found to distinguish the studied species, but do not separate genera, confirming that Spermacoceae is euripolynic.

Weed species is one of the factors contributing to grain yield losses due to crop-weed competition. Two of the most common weeds of soybean fields in Brazil are from the Rubiaceae family: Borreria latifolia and Richardia brasiliensis. This study aimed to evaluate the effects of B. latifolia and R. brasiliensis on plant development, yield components, and soybean productivity. Two assays were conducted in a randomized block design with four replications, using a two-level factorial design, in which the first factor consisted of the species B. latifolia and R. brasiliensis and the second factor consisted of the densities 0, 2, 4, 6, 8, 10, and 12 plants m-2. Plant height, leaf area and total chlorophyll in the V6 and R5 stages, number of pods per plant, number of grains per pod, 1000-grain weight, and grain yield loss were evaluated in both cultivars. Grain yield of both soybean cultivars was negatively affected by the interference with B. latifolia and R. brasiliensis. The species B. latifolia was more competitive with soybean when compared to R. brasiliensis, causing higher reductions for all variables. Each B. latifolia plant per square meter is able to reduce the yield of soybean by 3 a 4.4%, while each R.brasiliensis plant reduces yield by 2 a 2.6%. For both species, the reduction in soybean yield due to competition was caused by a decrease in the number of pods per plant, number of grains per pod, and 1000-grain weight.

Knowledge of factors that influence the germination process and their interactions allows estimating the initial development of a species in a particular environment and optimizing the weed management decisions. The objectives of this study were to evaluate the effect of environmental factors on the germination and initial growth of the weed species Borreria latifolia, Galianthe chodatiana and Richardia brasiliensis. Five trials were conducted using a completely random experimental design, in factorial design, with five repetitions, each one evaluating effects of temperature, irradiance, aluminum, salinity and pH. The germination was assessed daily, and germination speed index and the length of radicle and epicotyl were determined. Temperatures between 20 and 30 °C maximized final germination and germination speed index of the three species. B. latifolia showed low germination with 15 °C temperature. G. chodatiana and R. brasiliensis did not germinate well at temperatures between 30 and 35 °C. B. latifolia and R. brasiliensis did not germinate in the dark condition, whereas G. chodatiana were indifferent to photoperiod. Seeds from B. latifolia and G. chodatiana were more tolerant to aluminum concentration in the germination than R. brasiliensis, while G. chodatiana were tolerant in germination and early growth. Low NaCl concentration inhibits the seed germination of the three species. B. latifolia showed higher adaptation at low pHs, while the other species at pHs close to neutrality. The responses of the species to environmental factors can be used in the planning of strategies for their management.

Research by title the effect inundation period to summed dominant ratio (SDR) and biomass rice weeds of method SRI (system of rice intensification) in Indonesia. Research have been conducted in the Faculty of Agricultural Land Andalas University, Limau Manih, Padang, from February to May 2018. The study aims to identify noxious weed found in SRI method of paddy cultivation in Indonesia. Weeds that have summed Dominance Ratio (SDR) and the highest biomass in this study is a weed Cyperus rotundus, Scirpus juncoides Roxb., Fimbristylis miliacea (L.) Vahl, Cyperus pedunculatus and Richardia brasiliensis Gomez. Highest weed biomass obtained in the treatment of inundation 3 days old and weed biomass lowest is 15 days long inundation.

The medicinal species Richardia brasiliensis Gomes has secondary metabolites with considerable pharmacological activities. This study aims to determine the antiproliferative and genotoxic capacity of extracts and fractions obtained from the aerial parts of R. brasiliensis , in the Allium cepa test, on which one can detect the damages on chromosomes and the infl uence on cell proliferation during the mitotic cycle. The antiproliferative and genotoxic eff ect from extracts and fractions were evaluated at concentrations of 10 and 500 µg/mL and the mitotic index (MI) and the percentage of chromosomal alterations (CA) were calculated and used on the χ² test. It was observed antiproliferative capacity, the best results were from the autumn crude extract, and the hexane and butanol fractions with lower MI than the negative control. All treatments were genotoxic with CA, but with lower values than the ones found in the positive control.

Increased use of glyphosate in transgenic soybean areas has selected resistant and tolerant weed species. The aim of this study was to evaluate chemical management strategies for controlling Borreria latifolia and Richardia brasiliensis at pre-emergence (Pre), early post-emergence (Poste) and late post-emergence (Postl). Six experiments were carried out in a completely randomized design with four replicates per treatment in the Pre experiments and three in the Poste and Postl experiments, for each of the species. In the Pre experiments, tests were performed with herbicides imazethapyr, sulfentrazone, chlorimuron, diclosulam, S-metolachlor and saflufenacil. In the Poste experiments, seedlings were sprayed with herbicides bentazon, fomesafen, lactofen, flumioxazin and glyphosate. In Postl experiments, adult plants received glyphosate application associated with herbicides 2,4-D, carfentrazone, imazethapyr, flumiclorac, flumioxazin, sulfentrazone, chlorimuron, saflufenacil and glufosinate, plus three sequential applications with glyphosate only and paraquat/diuron. In the experiments, there was a control treatment without application of herbicides. In the Pre experiments, the plants established at 14 and 28 days after application (DAA) were evaluated. In the Poste and Postl experiments, shoot dry matter evaluation and visual control were performed at 14 and 28 DAA. The herbicides sulfentrazone, S-metolachlor and saflufenacil suppressed the emergence of both B. latifolia as R. brasiliensis; chlorimuron-ethyl and diclosulam were effective only on R. brasiliensis. In Poste, fomesafen, lactofen and flumioxazin reached levels of control over 90% of plants of both species. In Postl, glyphosate associated with carfentrazone, flumiclorac, flumioxazin, chlorimuron-ethyl, saflufenacil, glufosinate, and sequential applications of glyphosate/glyphosate, glyphosate/paraquat+diuron, glyphosate+2,4-D/paraquat+diuron reached levels control higher than 95%. RESUMO: A intensificação do uso do herbicida glyphosate em áreas de soja RR tem selecionado espécies de plantas daninhas resistentes e tolerantes. Objetivou-se neste trabalho avaliar estratégias de manejo químico para o controle de Borreria latifolia e Richardia brasiliensis com aplicações em pré-emergência (Pré), pós-emergência inicial (Pósi) e pós-emergência tardia (Póst) dessas plantas. Foram conduzidos seis experimentos em delineamento inteiramente casualizado com quatro repetições nos experimentos em Pré e três em Pósi e Póst, para cada uma das espécies. Nos experimentos em Pré, foram testados os herbicidas imazethapyr, sulfentrazone, chlorimuron, diclosulam, S-metolachlor e saflufenacil. Em Pósi, foram aplicados bentazon, fomesafen, lactofen, flumioxazin e glyphosate. Em Póst, plantas adultas receberam aplicações de glyphosate associadas a 2,4-D, carfentrazone, imazethapyr, flumiclorac, flumioxazin, sulfentrazone, chlorimuron, saflufenacil e glufosinate, além de três aplicações sequenciais com glyphosate isolado e paraquat/diuron. Nos experimentos havia um tratamento testemunha sem aplicação de herbicidas. Nos experimentos Pré, avaliou-se o percentual de plantas emergidas aos 14 e 28 dias após a aplicação (DAA). Em Pósi e Póst foram avaliados o controle visual aos 14 e 28 DAA e a matéria seca da parte aérea. Os herbicidas sulfentrazone, S-metolachlor e saflufenacil suprimiram a emergência tanto de B. latifolia quanto de R. brasiliensis; chlorimuron e diclosulam foram eficientes somente sobre R. brasiliensis. Em Pósi, fomesafen, lactofen e flumioxazin atingiram níveis de controle superiores a 90% sobre ambas as espécies. Em Póst, a associação de glyphosate com carfentrazone, flumiclorac, flumioxazin, chlorimuron, saflufenacil e glufosinate e as aplicações sequenciais de glyphosate/glyphosate, glyphosate/paraquat+diuron, e glyphosate+2,4-D/paraquat+diuron atingiram níveis de controle superiores a 95%.

One of the herbicides that can be used to control weeds on corn cultivation is the atrazine herbicide. The aim of this study was to determine the effectiveness of atrazine in controlling weeds in corn, to determine whether there was a change in the composition of weeds in corn after application of the atrazine, and to determine whether the use of atrazine herbicide could cause phytotoxicity and inhibit growth and reduce corn yield. Th e research was conducted on farmer's land in Hajimena Village, Natar District, South Lampung Regency and Weed Laboratory, Faculty of Agriculture, University of Lampung from November 2019 to March 2020. The research was arranged in a randomized complete block design (RCBD) with four replications and six treatments, namely the herbicide atrazine dose s of 1,20 kg/ha,1,80 kg/ha, 2,40 kg / ha, 3,00 kg / ha, manual weeding , and control. The results showed that atrazine herbicide doses of 1,20-3,00 kg/ha were effective to contro l of weeds total o n corn area up to 6 weeks after application, atrazine herbicide caused chang e o n weed composition of Richardia brasiliensis bec o me Calopogonium mucunoides at 3 and 6 weeks after application, and the application of atrazine herbicide doses of 1,20–3,00 kg/ha did not cause phytotoxicity , inhibit corn growth , and decrease yields. Keywords : atrazine, weed, herbicide, corn

The objective of this study was to evaluate the effects of nozzle types and 2,4-D formulations on spray deposition on different targets. Two field experiments were carried out in a completely randomized design, and treatments were arranged in a factorial scheme. Species in experiment 1 were Sumatran fleabane (Conyza sumatrensis) and Brazil pusley (Richardia brasiliensis) and in experiment 2 were soybeans (Glycine max) and Benghal dayflower (Commelina benghalensis). For both experiments, the first factor corresponded to spray nozzles with different settings (AD 110.015 - 61 and 105 L ha-1; AD 015-D - 75 and 146 L ha-1; XR 110.0202 - 200 L ha-1; and ADIA-D 110.02 - 208 L ha-1) and the second factor consisted of two formulations of 2,4-D (amine and choline). The formulation of 2,4-D choline has contained Colex-D™ Technology. Similar or higher spray deposition was observed on the leaves and artificial targets when using 2,4-D choline as compared to the 2,4-D amine formulation, and these differences in deposition were more evident for nozzles applying lower spray volumes. Deposition was more affected by nozzle type when amine formulation was used, compared to choline formulation.

An appropriate weed control is one of determining factors of agriculture crops growing. Effective of weeds control technics be dependent on kinds of crops and the weeds. The research of weeds vegetation analyses of cabbages unit land had been carried out in Education Farming Land of Universitas Quality Berastagi for three months, March to May 2017. The quadrangular method was used, by accounting the densities, dominances, frequencies, and important index value each of the weeds. Identification of weeds was done by using plants’ key determination guidance, by comparing the weeds that was discovered in the research location and the guidance, as well as the identically pictures of the weeds in certain references. Results of the research shown, there are 7 weeds were found, kentangan, grintingan, Goletrak beuti (Richardia brasiliensis Gomez), bebandotan (Ageratum conyzoides L), padang teguh (Themede arguens L. Hack), and ubi jalar (ipomea sp). The highest value of SDR (Summed Dominance Ratio) was Grintingan (28%) and the lowest was goletrak beuti (4.7%)

Infesting species of Rubiaceae family have great importance in soybean crops in Southwest of Paraná and North of Santa Catarina, especially due to the selection of tolerant populations caused by the pressure of selection exerted by intensive use of glyphosate. The study aimed to evaluate the levels of tolerance to glyphosate in biotypes of broadleaf buttonweed (Borreria latifolia) (BL) and white-eye (Richardia brasiliensis) (RB) by means of dose-response curves. Two experiments were conducted in a greenhouse, in completely randomized design (CRD), with four replications. Fourteen BL biotypes and eleven RB biotypes were evaluated, collected in soybean fields of Paraná and Santa Catarina. The doses of glyphosate were 0, 74, 163, 360, 792 and 1,742 g ha-1, applied when the plants had 6 to 8 leaves. Visual control was evaluated at 14 and 28 days after application (DAA), as well as mass of green shoots and mass of dry shoots at 28 DAA. It was verified that there is response variability among the biotypes studied. BL biotypes 277, 283 and 300, and RB biotypes 283, 285 and Papanduva were not controlled with glyphosate at doses superior to those normally used in crops (720 g ha-1), indicating selection by the recurrent use of glyphosate. The tolerance factors ranged from 1.1 to 4.1 and 2.8 to 8.1 for RB and BL, respectively. The greatest difficulties of control were reported in areas where RR technology had been adopted for more than six years. RESUMO: Espécies infestantes da família Rubiaceae apresentam grande importância em lavouras de soja no sudoeste do PR e norte de SC, especialmente devido à seleção de populações tolerantes ocasionada pela pressão de seleção exercida pelo uso intensivo do herbicida glyphosate. Este trabalho objetivou avaliar os níveis de tolerância ao glyphosate em biótipos de erva-quente (Borreria latifolia) (BL) e poaia-branca (Richardia brasiliensis) (RB) por meio de curvas de dose-resposta. Dois experimentos foram conduzidos em casa de vegetação, em delineamento inteiramente casualizado (DIC) com quatro repetições. Foram avaliados 14 biótipos de BL e 11 biótipos de RB coletados em lavouras de soja do PR e SC. As doses de glyphosate foram de 0, 74, 163, 360, 792 e 1.742 g ha-1, aplicadas quando as plantas estavam com 6 a 8 folhas. Avaliou-se o controle visual aos 14 e 28 dias após a aplicação (DAA), e a massa da parte aérea verde e seca, aos 28 DAA. Há variabilidade de resposta entre os biótipos estudados. Os biótipos de BL 277, 283 e 300 e os biótipos de RB 283, 285 e Papanduva não foram controlados com o glyphosate em doses superiores à usualmente utilizada nas lavouras (720 g ha-1), evidenciando seleção pelo uso repetitivo do glyphosate. Os fatores de tolerância variaram de 1,1 a 4,1 e 2,8 a 8,1 para BL e RB, respectivamente. As maiores dificuldades de controle foram relatadas em áreas onde a tecnologia RR tinha sido adotada há mais de seis anos.

The whitefly can be hosted by weeds and cause damage to cotton crops. The objective of this work was to identify which species of weeds among the floristic compositions occurring in the cotton crop are hosts of whiteflies (Bemisia tabaci biotype B). Bi-weekly evaluations were performed in a cotton area (FM 975 WS) in Sinop, Mato Grosso, Brazil, during the reproductive phase of the crop. An inventory square (0.25 m side) was randomly cast 15 times between the cotton rows. The weeds were cut close to the ground, conditioned and taken to the laboratory to identify the family and species, and to quantify the eggs and nymphs of whiteflies. Eleven families were detected, with the most frequent being Amaranthaceae (16.67%), Convolvulaceae (12.5%), Rubiaceae (12.5%) and Poaceae (12.5%). The most frequent species were Amaranthus spp. (13.79%) and Alternanthera tenella, Ipomea spp., Richardia brasiliensis and Eleusine indica, with 10.34% each. From the 15 collected species, the presence of whitefly eggs or nymphs was only not observed in Portulaca oleracea and E. indica. The highest incidence of B. tabaci occurred in Euphorbia heterophylla. The occurrence of these species should be monitored, so that the whitefly population does not interfere in the cotton fiber quality. RESUMO: A mosca-branca pode hospedar-se em plantas daninhas e causar prejuízos ao algodoeiro. O objetivo deste trabalho foi identificar, entre a composição florística de plantas daninhas que ocorrem na cultura do algodoeiro, quais são hospedeiras da mosca-branca (Bemisia tabaci biótipo B). Em uma área de algodoeiro (FM 975 WS) em Sinop, Mato Grosso, Brasil, foram feitas avaliações quinzenais na fase reprodutiva da cultura. Um quadrado inventário (0,25 m de lado) foi lançado aleatoriamente 15 vezes nas entrelinhas do algodoeiro. As plantas daninhas foram cortadas rente ao solo, acondicionadas e levadas para o laboratório para identificação da família e da espécie, quantificação dos ovos e ninfas de mosca-branca. Foram detectadas 11 famílias, sendo as mais frequentes Amaranthaceae (16,67%), Convolvulaceae (12,5%), Rubiaceae (12,5%) e Poaceae (12,5%). As espécies mais frequentes foram Amaranthus spp. (13,79%) e Alternanthera tenella, Ipomea spp., Richardia brasiliensis e Eleusine indica, com 10,34% cada uma. Das 15 espécies coletadas, apenas em Portulaca oleracea e E. indica não foi constatada a presença de ovos ou ninfas de mosca-branca. A maior incidência de B. tabaci ocorreu em Euphorbia heterophylla. A ocorrência dessas espécies deve ser monitorada para que a população de mosca-branca não interfira na qualidade da fibra do algodoeiro.

Infesting species of Rubiaceae family have great importance in soybean crops in Southwest of Paraná and North of Santa Catarina, especially due to the selection of tolerant populations caused by the pressure of selection exerted by intensive use of glyphosate. The study aimed to evaluate the levels of tolerance to glyphosate in biotypes of broadleaf buttonweed (Borreria latifolia) (BL) and white-eye (Richardia brasiliensis) (RB) by means of dose-response curves. Two experiments were conducted in a greenhouse, in completely randomized design (CRD), with four replications. Fourteen BL biotypes and eleven RB biotypes were evaluated, collected in soybean fields of Paraná and Santa Catarina. The doses of glyphosate were 0, 74, 163, 360, 792 and 1,742 g ha-1, applied when the plants had 6 to 8 leaves. Visual control was evaluated at 14 and 28 days after application (DAA), as well as mass of green shoots and mass of dry shoots at 28 DAA. It was verified that there is response variability among the biotypes studied. BL biotypes 277, 283 and 300, and RB biotypes 283, 285 and Papanduva were not controlled with glyphosate at doses superior to those normally used in crops (720 g ha-1), indicating selection by the recurrent use of glyphosate. The tolerance factors ranged from 1.1 to 4.1 and 2.8 to 8.1 for RB and BL, respectively. The greatest difficulties of control were reported in areas where RR technology had been adopted for more than six years.

The possibility of using auxin mimics herbicides in culture postemergence applications contributes to weed management. This study aimed at evaluating and comparing the effectiveness of 2,4-D and dicamba herbicides on glyphosate resistant or tolerant weeds. The treatments were dicamba and 2,4-D doses with or without glyphosate. The evaluated weeds in the experiment were Richardia brasiliensis, Commelina benghalensis, Conyza sp. and Ipomoea nil. Herbicides were applied when weeds were in 4 to 6 and 10 to 12 leaves stages. Data referring to evaluations on day 30 after application were submitted to analysis of variance. Response-dose curves were obtained for herbicides, and C85 and C95 values were identified. Weed species showed different tolerance to dicamba and 2,4-D, and differences were more evident for C. benghalensis. The addition of glyphosate to the auxin mimic reduced the differences between them. For plants in less developed stages, regardless of the species, mixtures of glyphosate + 2,4-D or glyphosate + dicamba provided excellent levels of control. In general, considering equivalent doses, for R. brasiliensis, C. benghalensis and I. nil, 2,4-D herbicide was superior to dicamba. As for Conyza bonariensis, dicamba showed better control percentages. RESUMO A possibilidade de utilização de herbicidas mimetizadores de auxina em aplicações de pós-emergência das culturas pode contribuir para o manejo da comunidade infestante. O presente trabalho teve por objetivos avaliar e comparar a eficiência dos herbicidas 2,4-D e dicamba sobre plantas daninhas resistentes ou tolerantes ao glyphosate. Os tratamentos utilizados foram doses de dicamba e 2,4-D associados ou não ao glyphosate. As espécies avaliadas foram Richardia brasiliensis, Commelina benghalensis, Conyza sp. e Ipomoea nil. Os herbicidas foram aplicados quando as plantas daninhas estavam com 4 a 6 folhas e 10 a 12 folhas. Os dados referentes às avaliações de controle aos 30 dias após a aplicação foram submetidos à análise de variância. Foram obtidas curvas de dose-resposta para os herbicidas e identificadas as doses para 80% e 95% (C80 e C95) de controle. As espécies avaliadas apresentaram suscetibilidade diferenciada ao dicamba e 2,4-D, sendo as diferenças mais evidentes para as plantas de C. benghalensis. A adição do glyphosate aos mimetizadores de auxina reduziu as diferenças de eficiência entre esses mimetizadores. Para plantas em estádios menos desenvolvidos, independentemente da espécie, as misturas de glyphosate+2,4-D ou glyphosate+dicamba proporcionaram excelentes níveis de controle. De maneira geral, considerando doses equivalentes, para as plantas de R. brasiliensis, I. nil e C. benghalensis, o herbicida 2,4-D mostrou-se superior ao dicamba. Já para Conyza bonariensis, o dicamba apresentou maiores porcentagens de controle.

Seven citrus orchards on reduced- to no-pesticide spray programs were sampled for predacious mites in the families Eupalopsellidae and Stigmaeidae (Acari: Prostigmata) in central and south central Florida. Inner and outer canopy leaves, fruit, twigs, and trunk scrapings were sampled monthly between August 1994 and January 1996. Open flowers were sampled in March from five of the sites. Two species of eupalopsellid mites (Exothorhis caudata Summers and Saniosulus harteni (van-Dis and Ueckermann)) were identified from 252 specimens collected within citrus tree canopies within the seven citrus orchards of which 249 were E. caudata. Only two E. caudata were collected from ground cover plants within five of the seven orchards. Eight species of Stigmaeidae were identified from 5,637 specimens: Agistemus floridanus Gonzalez, A. terminalis Gonzalez, Eustigmaeus arcuata (Chandhri), E. sp. near arcuata, E. segnis (Koch), Mediostigmaeus citri (Rakha and McCoy), Stigmaeus seminudus Wood, and Zetzellia languida Gonzalez were collected from within citrus tree canopies from seven orchard sites. Agistemus floridanus was the only species in either family that was abundant with 5,483 collected from within citrus tree canopies compared with only 39 from vine or ground cover plants. A total of 431 samples from one or more of 82 vines and ground cover plants were sampled monthly between September 1994 and January 1996 in five of these orchards and one or more eupalopsellids or stigmaeids were collected from 19 of these plants. Richardia brasiliensis (Meg.) Gomez had nine A. floridanus from 5 of 25 samples collected from this plant. Solanum sp. had five A. floridanus from three samples taken. Both eupalopsellid and stigmaeid species numbers represented

We aimed with this study to evaluate the potential of oilseed crops planted following soybean, in terms of their ability to inhibit the occurrence of weed species under Brazilian Savannah cropping systems. The long-term experiment was installed in 2010 at Dourados, MS, Brazil, and in completely randomized blocks design with five replications with 12 × 24 m plot size. For three consecutive years (2010/11, 2011/12 and 2012/13), soybeans were planted in all the area by October/November being harvested in February/March, when previously marked plots were planted in April with: (1) radish (Raphanus sativus); (2) rapeseed (Brassica napus); (3) crambe (Crambe abyssinica); (4) winter fallow-no plantation after soybeans. In all years the same crop was repeated at the same plots, with no crop rotation. Phytosociological characterization of weed species was carried out in winter (after oilseeds harvest), pre-planting and post-emergence of soybean for the three years. Relative abundance, frequency and dominance, as well as the importance value for each species, was obtained. Areas were also intra-characterized by the coefficients of Simpson, Shannon-Weiner and Shannon-Weiner’s Evenness Proportion, and areas were compared for species dissimilarity with Jaccard’s presence-only coefficient, by multivariate cluster analysis. Oilseed crops definitely contribute for sustainable weed management as a cultural tool. The most important oilseed crop weeds in the Savannah-like region of Brazil were Amaranthus hybridus, Richardia brasiliensis, Lepidium virginicum, Leonotis nepetifolia and Hybanthus parviflorus. After three years of repetitive crop succession, some weeds were selected, and rotation in Winter is advised at least every two or three years.

Herbicides are usually applied multiple times by growers for season long weed control in Florida citrus (Citrus sp.). Rimsulfuron, a sulfonylurea herbicide has been recently registered for control of certain grasses and broadleaf weeds in citrus. To increase the weed control spectrum and reduce application cost, citrus growers often prefer to tank mix herbicides. Field experiments were conducted in 2010 and 2011 in citrus groves in central Florida to evaluate weed control efficacy and crop safety of rimsulfuron applied alone or in tank mixes with flumioxazin, pendimethalin, or oryzalin. Herbicides were applied sequentially in spring and fall in both years on the same experimental plot. Results suggested that rimsulfuron applied alone controlled >80% broadleaf and grass weeds up to 30 days after treatment (DAT) and was comparable to tank mixing rimsulfuron with pendimethalin or oryzalin; however, control was reduced beyond 30 DAT. Rimsulfuron tank mixed with flumioxazin was the most effective treatment at 30 and 60 DAT that provided, respectively, ≥88% and >75%, control of broadleaf weeds including brazil pusley (Richardia brasiliensis), dog fennel (Eupatorium capillifolium), common ragweed (Ambrosia artemisiifolia), cotton weed (Froelichia floridana), and virginia pepperweed (Virginia virginicum) compared with other treatments. Control of natalgrass (Melinis repens) was higher in all tank mix treatments compared with rimsulfuron applied alone with no difference among tank mix partners. Rimsulfuron tank mixed with pendimethalin or oryzalin had no advantage over rimsulfuron applied alone for control of broadleaf weeds. Among sequential applications, weed control was better after fall herbicide application (August) compared with spring (April) because of residual activity of fall applied herbicides. Rimsulfuron tank mixed with flumioxazin will provide citrus growers with an additional weed control option.

Transgenic glyphosate-resistant soybeans (GRS) have been commercialized and grown extensively in the Western Hemisphere, including Brazil. Worldwide, several studies have shown that previous and potential effects of glyphosate on contamination of soil, water, and air are minimal, compared to those caused by the herbicides that they replace when GRS are adopted. In the USA and Argentina, the advent of glyphosate-resistant soybeans resulted in a significant shift to reduced- and no-tillage practices, thereby significantly reducing environmental degradation by agriculture. Similar shifts in tillage practiced with GRS might be expected in Brazil. Transgenes encoding glyphosate resistance in soybeans are highly unlikely to be a risk to wild plant species in Brazil. Soybean is almost completely self-pollinated and is a non-native species in Brazil, without wild relatives, making introgression of transgenes from GRS virtually impossible. Probably the highest agricultural risk in adopting GRS in Brazil is related to weed resistance. Weed species in GRS fields have shifted in Brazil to those that can more successfully withstand glyphosate or to those that avoid the time of its application. These include Chamaesyce hirta (erva-de-Santa-Luzia), Commelina benghalensis (trapoeraba), Spermacoce latifolia (erva-quente), Richardia brasiliensis (poaia-branca), and Ipomoea spp. (corda-de-viola). Four weed species, Conyza bonariensis, Conyza Canadensis (buva), Lolium multiflorum (azevem), and Euphorbia heterophylla (amendoim bravo), have evolved resistance to glyphosate in GRS in Brazil and have great potential to become problems.

Este trabalho teve como objetivos caracterizar a superfície foliar das plantas daninhas Commelina benghalensis, Ipomoea hederifolia, Richardia brasiliensis e Galinsoga parviflora e determinar a porcentagem de controle dessas plantas pelo herbicida glyphosate. As ceras epicuticulares das plantas daninhas foram extraídas com clorofórmio e quantificadas (µg cm²). Partes centrais das folhas foram submetidas à microscopia eletrônica de varredura, para caracterização da superfície foliar. A fim de avaliar a suscetibilidade dessas plantas daninhas ao glyphosate, foi instalado experimento inteiramente casualizado composto por sete tratamentos (0, 360, 540, 720, 900, 1.440 e 2.160 g e.a. ha-1 de glyphosate) e quatro repetições em casa de vegetação, na Universidade de São Paulo, ESALQ/USP, PiracicabaSP, Brasil. A eficácia do herbicida foi avaliada aos 14, 21 e 28 dias após aplicação dos tratamentos. As plantas daninhas não diferiram muito com relação à quantidade de ceras epicuticulares. Em G. parviflora a superfície foliar apresenta tricomas tectores multicelulares e estômatos anomocíticos. I. hederifolia apresenta superfície foliar rugosa, tricomas tectores unicelulares e glandulares e estômatos paracíticos. Em C. benghalensis, a superfície foliar apresenta dois tipos de tricomas tectores, estômatos tetracíticos e ceras dispersas na superfície adaxial. A planta daninha R. brasiliensis apresenta estômatos paracíticos e tricomas unicelulares. As plantas daninhas C. benghalensis e R. brasiliensis são mais tolerantes ao glyphosate do que as outras espécies estudadas. Com base nos dados obtidos, pode-se concluir que as plantas daninhas apresentam características foliares diferentes, sendo C. benghalensis e R. brasiliensis as mais tolerantes ao glyphosate, mesmo quando se utiliza a maior dose herbicida. This work aimed to characterize the foliar surface of the weeds Commelina benghalensis, Ipomoea hederifolia, Richardia brasiliensis and Galinsoga parviflora and the percentage of control by the herbicide glyphosate. The epicuticular waxes were extracted by chloroform and quantified (µg cm-2). Central parts of the leaves of these weeds were submitted to electron microscopy to characterize the foliar surface. To evaluate the susceptibility of these weeds to glyphosate an experiment was arranged in a randomized complete design, 7 treatments (0, 360, 540, 720, 900, 1440 and 2160 g a.e. ha-1 of glyphosate) and four replications under greenhouse conditions at the University of São Paulo - ESALQ/USP - Piracicaba-SP, Brazil. Herbicide efficacy was assessed at 14, 21 and 28 days after treatment. In G. parviflora the foliar surface presents multicellular trichomes, and anomocytic stomata. In I. Hederifolia, the foliar surface is rough, with multicellular and glandular trichomes and paracytic stomata. In C. benghalensis, the foliar surface presents two types of trichomes and a lower number of tetracytic stomata. The presence of disperse wax was observed on the adaxial surface. R. brasiliensis presents great number of unicellular trichomes and paracytic stomata. The weeds C. benghalensis and R. brasiliensis were more tolerant to glyphosate than the other species studied. Based on the data obtained, it can be concluded that the weeds showed differences in the foliar characteristics, with C. benghalensis and R. brasiliensis being tolerant to the highest dose of glyphosate.

– The chromosomes of 14 species belonging to two subfamilies of the family Rubiaceae collected in the Brazilian cerrado were studied. In the subfamily Ixoroideae, five species (Alibertia concolor, A. edulis, A. sessilis, Genipa americana and Tocoyena formosa) had a chromosome number of 2n = 22 and one (Amaioua intermedia) showed polyploidy, with 2n = 66. All of the chromosome numbers were multiples of the more common basic number for this family (x = 11). In the subfamily Rubioideae, variations in the number of chromosomes were observed among tribes and genera, with 2n = 20 in the tribe Coccocypseleae (Coccocypselum lanceolatum), 2n = 28 in Spermacoceae (Borreria latifolia, B. verticillata and Richardia brasiliensis) and n = 11 in Coussareeae (Coussarea hydrangeifolia). In the tribe Psychotrieae, the variations included n = 11 (Palicourea rigida), 2n = 22 (Psychotria hoffmannseggiana) and 2n = 32 (Psychotria deflexa). The ideograms elaborated for 12 species were symmetrical (TF% varied from 47.45...

Embora o herbicida glyphosate seja considerado não-seletivo, várias espécies de plantas daninhas apresentam certo grau de tolerância às doses recomendadas. No Brasil, já existem relatos de seleção de espécies de plantas daninhas tolerantes ao glyphosate em áreas com o uso intensivo deste herbicida. Assim, o objetivo desta pesquisa foi avaliar a influência da aplicação repetitiva do herbicida glyphosate sobre a dinâmica do banco de sementes das plantas daninhas tolerantes: Commelina benghalensis, Ipomoea grandifolia e Richardia brasiliensis; e das plantas daninhas suscetíveis: Amaranthus hybridus e Galinsoga parviflora. O experimento foi monitorado durante dois anos em áreas experimentais do Departamento de Produção Vegetal da Escola Superior de Agricultura ''Luiz de Queiroz'' - Universidade de São Paulo, Piracicaba, São Paulo. Partindo de uma densidade de 100 plantas m-2 de cada espécie, o glyphosate foi aplicado periodicamente em pós-emergência sobre as plantas daninhas adultas nas doses de 0, 0,36, 1,44 e 2,88 kg e.a. ha-1. O banco de sementes do solo foi estimado através da avaliação de emergência direta das plântulas e extração física das sementes. O banco de sementes das plantas daninhas tolerantes ao glyphosate teve aumento significativo, principalmente nas menores doses. Por outro lado, o banco de sementes das espécies suscetíveis diminuiu ao longo do tempo mesmo nas menores doses do glyphosate. Na última análise do banco de sementes, as plantas daninhas tolerantes apresentaram, na maior dose de glyphosate aplicada, cerca de 12 milhões de sementes não-dormentes ha-1, ao passo que as suscetíveis apresentaram cerca de 4 milhões de sementes não-dormentes ha-1. Concluiu-se que o uso intensivo do herbicida glyphosate pode ocasionar uma mudança na área, aumentando a freqüência das plantas tolerantes ao longo dos anos.

No ano agrícola 1996/97 foi conduzido, na Fazenda-Escola da Universidade Estadual de Ponta Grossa, em Ponta Grossa-PR, um experimento a campo com o objetivo de determinar o período crítico de prevenção da interferência das plantas daninhas sobre a cultura do feijoeiro-comum (Phaseolus vulgaris), em sistema de semeadura direta, associando esse período com a fenologia da planta. O delineamento experimental utilizado foi o de blocos ao acaso em arranjo fatorial 2 x 8, com quatro repetições. Os 16 tratamentos testados foram resultados da combinação de dois conjuntos de tratamentos de interferência das plantas daninhas: (1) inicialmente em convivência com as plantas daninhas (2) inicialmente sem convivência com as plantas daninhas, em sete estádios fenológicos do feijoeiro - V2, V3, V4, R5, R6, R7 e R8 - e uma testemunha em convivência durante todo o ciclo da cultura. O experimento foi instalado em uma área há oito anos sob plantio direto, com semeadura realizada de acordo com a tecnologia recomendada para a cultura, com adubações no sulco e em cobertura e tratamentos fitossanitários, para que os feijoeiros se desenvolvessem normalmente. O período crítico de prevenção da interferência ocorreu entre os estádios fenológicos V4 e R6, e a interferência das plantas daninhas durante todo o ciclo reduziu em média 71% o rendimento de grãos dos feijoeiros. Com relação à comunidade infestante, as dicotiledôneas representaram 61,3% das plantas daninhas, destacando-se as espécies Bidens pilosa e Richardia brasiliensis, com 30,6 e 16,6%, respectivamente; já as monocotiledôneas representaram 38,7% da comunidade infestante, com destaque para as espécies Digitaria horizontalis e Brachiaria plantaginea, com 23,6 e 14,3%, respectivamente.

Um experimento de campo foi conduzido em Presidente Castelo Branco-PR, com o objetivo de avaliar a seletividade do herbicida azafenidin para a cultura de Eucalyptus camaldulensis, assim como sua eficácia no controle de algumas plantas daninhas infestantes nessa cultura. Azafenidin foi aplicado nas doses de 300, 400, 500 600 e 800 g i.a. ha-1, em pré-emergencia das plantas daninhas. Tratamentos adicionais, incluindo oxyfluorfen (720 g i.a. ha-1), uma testemunha capinada e outra testemunha sem capina, foram também incluídos. Tanto azafenidin quanto oxyfluorfen provocaram sintomas de toxicidade na cultura; no primeiro, as injúrias foram mais intensas em doses ³ 600 g i.a. ha-1. Em relação ao controle de plantas daninhas, doses de azafenidin a partir de 500 g i.a. ha-1 foram efetivas no controle de Brachiaria decumbens, Commelina benghalensis, Richardia brasiliensis e Sida santaremnensis, mesmo 180 dias após a aplicação. Doses menores de azafenidin resultaram em controle suficiente da maioria das plantas daninhas, mas com menor efeito residual.

The genera Borreria G. Mey. and Richardia L. belong to Rubiaceae family. Borreria verticillata (vassoura-de-botão) presents indole and isoquinoline alkaloids, iridoids, sesquiterpenes and steroids. It is popularly used for diabetes, bacterial infections, eczema, rheumatism, analgesic and antipyretic. Borreria ocymoides is used to treat fungal infections and eczema and due to morphological and anatomical similarity with B. verticillata is believed that might be being used with the same purposes as B. verticillata. Although there are no reports in the chemical literature, preliminary phytochemical screening revealed the presence of saponins, tannins, glycosides and anthraquinones. Richardia brasiliensis (ervanço and poaia) presents terpenoids, flavonoids and coumarins. Ethnopharmacological studies indicate emetic, antidiabetic, anthelmintic properties and for the treatment of eczema, burns, bronchitis, influenza and hemorrhoid. This work contributed to the phytochemical family Rubiaceae using B. verticillata, B. ocymoides and R. brasiliensis. For this, the plants were collected, identified and deposited in the Prof. Lauro Pires Xavier (JPB) herbarium. Dried and pulverized, the powder plant was subjected to extraction and partitioning. Then, the phase chromatography was subjected to chromatographic and spectroscopic methods for isolation and identification of substances, respectively. B. verticillata revealed the predominance of secondary metabolites belonging to the class of terpenoids (β-sitosterol, stigmasterol, betulinic acid, alcohol betulinic, oleanolic acid, ursolic acid, uvaol and 3β-hydroxyurs-11-en-28,13-β-olide) and as porphyrin derivatives (pheophytin a, pheophytin b and 151-hydroxy-(151-S)-porphyrin lactone a). B. ocymifolia showed a predominance of phenolic substances as coumarins (scopoletin, umbelliferone and cumarieletephyn), anthraquinones (2-hydroxy-3-methoxy-6-metilantracen-9,10-dione and 2-hydroxy-3-metilantracen-9,10-dione), and p-hydroxycinnamic acid and ursolic acid, showing a different chemical profile to morphologically similar Borreria. R. brasiliensis showed the presence of terpenoids: ursolic acid and uvaol. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Os gêneros Borreria G. Mey. e Richardia L. pertencem a família Rubiaceae. Borreria verticillata (vassoura-de-botão) apresenta alcaloides indólicos e isoquinolínicos, iridoides, esteroides e sesquiterpenos e é utilizada popularmente para diabetes, infecções bacterianas, eczema, reumatismo, antitérmica e analgésica. Borreria ocymoides é utilizada no tratamento de micoses e eczemas e em decorrência da semelhança morfo-anatômica com B. verticillata acredita-se que possa estar sendo utilizada com as mesmas finalidades que esta última. Apesar de não haver relato na literatura do ponto de vista químico, triagens fitoquímicas preliminares revelaram presença de saponinas, taninos, glicosídios e antraquinonas. Richardia brasiliensis (ervanço e poaia) apresenta terpenóides, flavonóides e cumarinas. Estudos etnofarmacológicos indicam propriedades emética, antidiabética, vermífuga e para o tratamento de eczema, queimaduras, bronquite, gripe, hemorróida e malária avícola. Este trabalho objetivou contribuir com os estudos fitoquímicos da família Rubiaceae utilizando B. verticillata, B. ocymoides e R. brasiliensis. Para isto, as espécies vegetais foram coletadas, identificadas e depositadas no Herbário Prof. Lauro Pires Xavier (JPB). Após secagem e pulverização, o pó das plantas foi submetido a processos de extração e particionamento. Em seguida, a fase cromatográfica foi submetida a métodos cromatográficos e espectroscópicos para isolamento e identificação das substâncias, respectivamente. B. verticillata revelou o predomínio metabólitos secundários pertencentes à classe dos terpenoides (β-sitosterol, estigmasterol, ácido betulínico, ácool betulínico, ácido oleanólico, ácido ursólico, uvaol e 3β-hidroxiurs-11-en-28,13-β-olídio), bem como, derivados porfirínicos (feofitina a, feofitina b e 151-hidroxi-(151-S)-porfirinolactona a). B. ocymifolia apresentou predomínio de substâncias fenólicas uma vez que foram isoladas cumarinas (escopoletina, umbeliferona e cumarieletefina), antraquinonas (2-hidroxi-3-metoxi-6-metilantraceno-9,10-diona e 2-hidroxi-3-metilantraceno-9,10-diona), além do ácido p-hidroxicinâmico e ácido ursólico, mostrando um perfil químico diferente para essas borrérias morfologicamente semelhantes. R. brasiliensis demonstrou a presença dos terpenóides: ácido ursólico e uvaol.

Objetivou-se com este trabalho estudar a inter-relação entre a cultura de milho e cultivares de Urochloa spp. em consórcio, assim como a interferência de plantas daninhas nesses sistemas. O ensaio foi aleatorizado em três blocos em esquema de parcelas subdivididas, sendo as parcelas constituídas por sete sistemas de produção (Urochloa brizantha cv. 'Marandu' e cv. 'Piatã' e U. decumbens cv. 'Basilisk' em cultivo solteiro e em consórcio com o milho e cultivo solteiro de milho) e as subparcelas pelo controle de plantas daninhas (com e sem capina manual). Verificou-se que o estabelecimento dos consórcios de milho com as cultivares de Urochloa spp. não afeta os componentes de rendimento de produção, altura e produtividade do milho, assim como não reduz a comunidade de plantas daninhas, mas interfere na densidade populacional e massa de matéria seca por planta e por área das cultivares de Urochloa spp. Houve níveis de interferência semelhantes das plantas daninhas entre os sistemas de produção sem capina, devido à similaridade entre suas comunidades de plantas daninhas, com predomínio de espécies consideradas altamente competitivas, como Panicum maximum, e à predominância de espécies como Richardia brasiliensis e Ageratum conyzoides, associadas ao controle com capina. This research was carried out to study the interaction between the corn and Urochloa spp. cultivars in association, as well as the interference of weeds in this cropping system. A randomized experiment in three blocks with split-plots design was conducted. The plots consisted of seven cropping systems (Urochloa brizantha cv. 'Marandu' and cv. 'Piatã' and U. decumbens cv. 'Basilisk' a sole culture and in association with corn as well as the sole culture of corn) and the split-plots by weed control (with and without weeding). It was found that the establishment of corn-Urochloa spp. cultivars association does not affect yield components such as height and grain yield, nor reduces the weed community, but interferes in the density, mass of dry matter per plant and per area of Urochloa spp. cultivars. There were similar levels of interference of weeds between cropping systems without weeding due to the similarity between the weed communities, showing the predominance of species considered as highly competitive such as Panicum maximum and also due to the low infestation with predominance of species such as Richardia brasiliensis and Ageratum conyzoides, associated with weed control.

Made available in DSpace on 2015-02-02T12:39:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-03-01Bitstream added on 2015-02-02T13:08:02Z : No. of bitstreams: 1 S1808-16572012000100019.pdf: 244304 bytes, checksum: 34d627020454e880ed3aec3f55b4f843 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O objetivo do presente trabalho foi avaliar a eficiência de diferentes herbicidas, aplicados em jato dirigido, no controle de plantas daninhas dicotiledôneas na cultura da mamona. Os herbicidas testados foram: saflufenacil (28, 40 e 56 g ha-1), carfentrazone-ethyl (30 g ha-1), flumioxazin (25 g ha-1), glyphosate (720 g ha-1) e glyphosate + saflufenacil (720 + 28 g ha-1) aplicados em pós-emergência em três espécies de plantas daninhas (Richardia brasilienses, Sida rhombifolia e Ipomoea grandifolia), além de uma testemunha sem aplicação de herbicida. Os herbicidas foram pulverizados quando as plantas de mamona apresentavam 0,50 a 0,70 m de altura. As avaliações visuais de controle foram realizadas aos 3, 7, 14, 21 e 28 dias após a aplicação dos tratamentos. O estudo foi instalado no delineamento experimental em blocos casualizados, com três repetições. O herbicida saflu-fenacil foi eficiente no controle de todas as plantas daninhas estudadas, sendo que os herbicidas carfentrazone-ethyl e flumioxazin obtiveram apenas controles satisfatórios das plantas daninhas. A mistura testada foi eficiente no controle de todas as plantas daninhas. The objective of this study was to evaluate the effectiveness of different herbicides, applied in direct spray, to control dicotyledonous weeds in castor beans. The herbicides tested were saflufenacil (28, 40 and 56 g ha-1), carfentrazone-ethyl (30 g ha-1), flumioxazin (25 g ha-1), glyphosate (720 g ha-1) and glyphosate + saflufenacil (720 + 28 g ha-1) applied post-emergence on three species of weeds (Richardia brasiliensis, Sida rhombifolia and Ipomoea grandifolia), and a control without herbicide. The herbicides were sprayed when the castor bean plants showed 50 to 70 cm of height. Visual assessments of the degree of control were performed at 3, 7, 14, 21 and 28 days after treatment application. The study was installed in a randomized complete block design with three replications. The herbicide saflufenacil was effective in the control of all the weeds studied, and the herbicides carfentrazone-ethyl and flumioxazin presented only satisfactory control of weeds. The mixture tested was effective in the control of all weeds. Universidade Estadual Paulista Faculdade de Ciências Agrárias Departamento de Produção Vegetal Universidade Estadual Paulista Faculdade de Ciências Agrárias Departamento de Produção Vegetal

Various combinations of glyphosate and 2,4-D (± surfactant) were evaluated for control of Brazil pusley [Richardia brasiliensis (Moq.) Gomez]. Typical 2,4-D symptoms on plants were manifested within 2 to 3 days after treatment. Application of glyphosate alone had only marginal effects (14%) on Brazil pusley, but the addition of Induce® (nonionic surfactant) significantly increased control to 83% and reduced the fresh weight by 68%. Application of Landmaster®II or a tank-mix of glyphosate + 2,4-D (± surfactants) resulted in 96% to 100% control. Treatment with 2,4-D alone, or with Induce®, or L-77® (organosilicone surfactant) resulted in 84%, 90%, or 100% control, respectively. Very low fresh weights of Brazil pusley were recorded when 2,4-D +Induce® or L-77®, Landmaster®II (± surfactants), or the tank-mix (± surfactants) were applied. In the regrowth studies, shoot weight was greater following application of glyphosate with or without L-77® or Kinetic® (a blend of nonionic and organosilicone) than following other treatments. The fresh weight of the shoots in the regrowth study, recorded following the application of 2,4-D or Landmaster®II (± surfactants), was very low except when Kinetic® was added to Landmaster®II. No regrowth of shoots occurred following the tank-mix treatment. Similar observations were recorded for roots. Plants treated with 2,4-D did not regrow. The presence of 2,4-D in either formulation accelerated synergistic effect of the glyphosate to the target site. Therefore, 2,4-D could be used either as a component of a formulation or in a tank-mix with glyphosate to control Brazil pusley. Chemical names used: N-(phosphonomethyl glycine) (glyphosate); 2,4-dicholorophenoxyacetic acid (2,4-D).

Richardia brasiliensis é uma planta utilizada na medicina popular como expectorante, emética, diaforética, vermífuga e no tratamento de hemorróidas. O objetivo desse trabalho foi obter parâmetros de controle de qualidade para a identificação da droga vegetal constituída pelas partes aéreas e pela raiz da R. brasiliensis, dados ainda não descritos na literatura. Os resultados indicaram que a folha é anfiestomática, apresentando estômatos paracíticos e tricomas tectores unicelulares. A nervura principal apresentou feixe vascular colateral, o pecíolo feixe vascular em forma de arco e dois pequenos feixes vasculares laterais e a raiz principal apresentou parênquima cortical com grande quantidade de grãos-de-amido e idioblastos contendo ráfides de oxalato de cálcio. Na prospecção fitoquímica das raízes e das partes aéreas verificou- se a presença de cumarinas, resinas, esteróides e triterpenóides e somente nas partes aéreas flavonóides e alcalóides. Os resultados obtidos nesse estudo podem auxiliar na identificação e controle de qualidade dessa matéria-prima vegetal., Richardia brasiliensis is a plant used in folk medicine as expectorant, emetic, diaphoretic, vermifuge and to treat hemorrhoids. The goal of this research was to obtain quality control parameters for the identification of the plant drug of R. brasiliensis aerial parts and roots, data not reported in the literature. The results indicated that the leaf presents paracytic stomata in both surfaces and unicellular non-glandular trichome. The midrib presents collateral vascular bundle, the petiole possess vascular bundle in arc form and two small lateral vascular bundle, and the main root presents the cortical parenchyma with great amount of starches grains and idioblasts with calcium oxalate raphides. In the phytochemical screening, coumarin, resin, steroid and triterpenoid were detected in aerial parts and roots and only in the aerial parts were detected flavonoids and alkaloids. These results may help in the identification and quality control of this raw material plant, Colegio de Farmacéuticos de la Provincia de Buenos Aires

The family Rubiaceae comprises around 637 genera and approximately 10,700 species, occurring essentially in tropical regions of Brazil. Richardia brasiliensis Gomes, known popularly as "poaia branca", is native to Brazil south region, used in folk medicine as anti-emetic and in the treatment of diabetes. This work reports the isolation and structural identification of a flavonoid glycoside, a triterpene, a coumarin and two benzoic acid derivatives, aiming at contributing to the chemotaxonomy of the genus Richardia, through a phytochemical study of Richardia brasiliensis. By means of this study the metabolites isorhamnetin-3-O-rutinoside, oleanolic acid, the coumarin scopoletin and p-hydroxy-benzoic and m-methoxy-p-hydroxy-benzoic acids were isolated and identified. All of them, but the latter, were isolated for the first time in the genus, thereby presenting relevant chemotaxonomic importance to it. The structures were identified using spectroscopic techniques such as IR, one and two-dimensional ¹H and 13C NMR besides comparison with literature data. A família Rubiaceae compreende cerca de 637 gêneros e aproximadamente 10700 espécies, ocorrendo essencialmente nas regiões tropicais do Brasil. Richardia brasiliensis Gomes, popularmente conhecida por "poaia branca", é uma planta nativa da região sul do Brasil, utilizada na medicina popular como anti-emética e no tratamento de diabetes. Este trabalho reporta o isolamento e identificação estrutural de um flavonóide glicosilado, um triterpeno, uma cumarina e dois derivados de ácido benzóico, objetivando contribuir para quimiotaxonomia do gênero Richardia. Através deste estudo foi possível isolar e identificar os metabólitos isorametina-3-O-rutinosídeo, ácido oleanólico, a cumarina escopoletina e os ácidos p-hidroxi-benzóico e m-metoxi-p-hidroxi-benzóico, todos isolados pela primeira vez no gênero, exceto o último, apresentando, portanto, relevante importância quimiotaxonômica para o mesmo. As estruturas foram identificadas com o uso de técnicas espectroscópicas de IV, RMN ¹H e 13C uni e bidimensionais e comparação com dados da literatura.

The effects of competition of six weed species on growth, nutrient concentration and nutrient content of coffee plant root system under greenhouse conditions were evaluated. Thirty days after coffee seedling transplantation into 12 L pots with soil level area of 6.5 dm². Weeds were transplanted or sowed in these pots, at densities of 0, 1, 2, 3, 4 and 5 plants per pot. The duration of competition (or weedy periods) from weed transplantation or emergence until plant harvesting, at the weed preflowering stage, were (in days): 77 (Bidens pilosa), 180 (Commelina diffusa), 82 (Leonurus sibiricus), 68 (Nicandra physaloides), 148 (Richardia brasiliensis) and 133 (Sida rhombifolia). Dry matter of coffee plants was linearly reduced with increasing B. pilosa and S. rhombifolia density, with pronounced effect of B. pilosa. C. diffusa was the only weed species whose increasing density in the pots did not diminish crop root dry matter. L. sibiricus, N. physaloides and R. brasiliensis reduced root dry matter of coffee plants by 75, 52 and 47%, respectively, as compared to the weed-free treatment, regardless of weed density. Under competition, even though weed species showed lower macronutrient concentration in the roots (except for P), they accumulated 4.2 (N), 12.3 (P), 4.3 (K), 5.5 (Ca), 7.6 (Mg) and 4.4 (S) times more nutrients in the roots than the coffee plants. Crop and weed nutrient concentration, as well as competition degrees greatly varied depending on both weed species and densities. Este trabalho teve como objetivo avaliar os efeitos da competição de seis espécies de plantas daninhas sobre o crescimento, a concentração e o conteúdo de nutrientes no sistema radicular de plantas de café, cultivadas em casa de vegetação. Aos 30 dias após o transplantio das mudas de café, em vasos contendo 12 L de substrato e com área de 6,5 dm² na superfície do solo, fez-se o transplantio e, ou, a semeadura das espécies daninhas nesses vasos, em seis densidades (0, 1, 2, 3, 4 e 5 plantas por vaso). Os períodos de convivência, desde o transplantio ou emergência das plantas daninhas até a colheita das plantas, quando do florescimento das plantas daninhas, foram (em dias): 77 (Bidens pilosa), 180 (Commelina diffusa), 82 (Leonurus sibiricus), 68 (Nicandra physaloides), 148 (Richardia brasiliensis) e 133 (Sida rhombifolia). A massa seca do sistema radicular das plantas de café reduziu-se linearmente com o aumento da densidade de B. pilosa e de S. rhombifolia, com efeito mais pronunciado em B. pilosa. C. diffusa foi a única espécie que não reduziu o acúmulo de matéria seca no sistema radicular das plantas de café. L. sibiricus, N. physaloides e R. brasiliensis reduziram a massa seca da raiz do café em 75, 52 e 47%, respectivamente, comparado ao tratamento livre de interferência, independentemente da densidade de plantas daninhas. Sob competição, apesar de apresentarem, no seu sistema radicular, concentrações de macronutrientes (exceto para P) inferiores àquelas do café, as plantas daninhas acumularam 4,2 (N), 12,3 (P), 4,3 (K), 5,5 (Ca), 7,6 (Mg) e 4,4 (S) vezes mais nutrientes nas raízes que as plantas de café. A concentração de nutrientes no café e nas plantas daninhas, assim como o grau de interferência dessas plantas, variaram fortemente com a espécie e densidade de planta daninha.

"In the humid forest regions of southern Cameroon in central Africa, sectoral and macroeconomic policy reforms introduced in the late 1980s have led to intensified land use, which in turn has resulted in, among other environmental consequences, shortened fallow systems dominated by the Asteraceae shrub, Chromolaena odorata (L.) King and Robinson, rather than by secondary forest species. A trial was established to determine the effect of shortened fallow duration and invasion by C. odorata on the weed flora in subsequent mixed food cropping systems. Plots were established in cleared 5- to 7-year-old fallow fields in which the vegetation was either dominated by C. odorata or not, and in which the dominant fallow vegetation in the previous crop-fallow rotation had been either C. odorata, forest, or herbaceous (not dominated by C. odorata). Cassava (Manihot esculenta Crantz), maize (Zea mays L.), and groundnuts (Arachis hypogaea L.) were intercropped and weed species were assessed 6, 14, and 30 weeks after crop planting. Soil analyses were conducted to assess the influence of edaphic traits on the distribution and abundance of dominant weed species. The results clearly indicated an enrichment of the weed flora with time after planting, but little difference between fallow histories. Two groups of weed species corresponded with soil characteristics: C. odorata, Cyathula prostrata, Mariscus alternifolius, Mikania cordata, Musanga cecropioides, and Trema orientalis were preponderant on soils with high clay, N, and C contents, and Ageratum conyzoides, Cyperus sp., Haumania danckelmaniana, Paspalum conjugatum, Pouzolzia guineensis, Richardia brasiliensis, Sida rhombifolia, Stachytarpheta cayennensis, Talinum triangulare, and Triumfetta cordifolia were preponderant on sandier soils with high pH, P, and Mg contents."

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:51:31Z No. of bitstreams: 1 S0100-83582004000100007.pdf: 126510 bytes, checksum: 3dc5be71beca2e6875b03d8716e33819 (MD5) Made available in DSpace on 2013-08-22T18:51:31Z (GMT). No. of bitstreams: 1 S0100-83582004000100007.pdf: 126510 bytes, checksum: 3dc5be71beca2e6875b03d8716e33819 (MD5) Previous issue date: 2004-03-01 Made available in DSpace on 2013-09-30T17:50:09Z (GMT). No. of bitstreams: 2 S0100-83582004000100007.pdf: 126510 bytes, checksum: 3dc5be71beca2e6875b03d8716e33819 (MD5) S0100-83582004000100007.pdf.txt: 31403 bytes, checksum: c94ce296de89434fca52588226830148 (MD5) Previous issue date: 2004-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:13:25Z No. of bitstreams: 2 S0100-83582004000100007.pdf: 126510 bytes, checksum: 3dc5be71beca2e6875b03d8716e33819 (MD5) S0100-83582004000100007.pdf.txt: 31403 bytes, checksum: c94ce296de89434fca52588226830148 (MD5) Made available in DSpace on 2014-05-20T13:13:25Z (GMT). No. of bitstreams: 2 S0100-83582004000100007.pdf: 126510 bytes, checksum: 3dc5be71beca2e6875b03d8716e33819 (MD5) S0100-83582004000100007.pdf.txt: 31403 bytes, checksum: c94ce296de89434fca52588226830148 (MD5) Previous issue date: 2004-03-01 Foram realizados dois experimentos, em condições de casa de vegetação, no Departamento de Biologia Aplicada à Agropecuária da FCAV-UNESP de Jaboticabal, objetivando-se determinar o acúmulo de massa seca, assim como a distribuição e o acúmulo de macronutrientes durante os ciclos de vida de plantas de soja cultivar BR16, no período de outubro de 2000 a fevereiro de 2001, e de Richardia brasiliensis (poaia-branca), uma planta daninha de elevada importância para esta cultura no Brasil, especialmente em áreas de plantio direto, no período de outubro de 1998 a fevereiro de 1999. Os estudos foram realizados em delineamento experimental inteiramente casualizado, com quatro repetições. Quatro plantas cresceram em vasos com capacidade de sete litros, preenchidos com areia de rio lavada, peneirada e irrigada diariamente com solução nutritiva. Os tratamentos foram representados pelas épocas de amostragem, realizadas a intervalos de 14 dias, a saber: 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162 e 176 dias após a emergência (DAE) das plantas de R. brasiliensis; e 21, 35, 49, 63, 77, 91, 105 e 119 DAE das plantas de soja cv. BR-16 (precoce). em ambas as plantas, as folhas tiveram a maior partição de biomassa durante sete semanas. Para este dado, a partição foi maior para as estruturas reprodutivas em soja e nos caules para a poaia-branca. O ponto de máximo acúmulo teórico de massa seca deu-se aos 104 DAE para a soja (36,6 g por planta) e aos 146 DAE para a poaia-branca (16,4 g por planta). da emergência até aos 50 DAE as folhas apresentaram maior participação no acúmulo de massa seca, nas duas espécies. Após 50 DAE notou-se, em ambas as espécies, uma inversão na representatividade das folhas por caules, para a espécie daninha, e por caules e posteriormente por estruturas reprodutivas, para a cultura. A taxa de absorção diária dos macronutrientes atingiu maiores valores entre 69 e 87 DAE para a soja e entre 106 a 111 DAE para a planta daninha. Levando em conta a média dos valores de pontos de inflexão observados para a cultura da soja, aos 78 DAE uma planta de soja acumula teoricamente 25,9 g de massa seca; 615,5 mg de N; 77,2 mg de P; 538,6 mg de K; 535,0 mg de Ca; 171,5 mg de Mg; e 39,5 mg de S. Para o mesmo período, uma planta de R. brasiliensis acumula teoricamente 3,7 g de massa seca; 50,8 mg de N; 3,2 mg de P; 104,4 mg de K; 127,8 mg de Ca; 18,8 mg de Mg; e 3,7 mg de S. Two greenhouse trials were carried out under greenhouse conditionsat the Department of Agricultural Biology at FCAV-UNESP in Jaboticabal, SP, Brazil to study tbiomass accumulation as well as macronutrient distribution and accumulation during the life cycle of the soybean cultivar BR 16 from October 2000 to February 2001, and Richardia brasiliensis (Brazilian pusley), from October 1998 to February 1999. Brazilian pusley is one of the most important weeds in the mid western soybean growing area, mainly under no tillage system. The studies were conducted in a randomized complete design with four replications. Four plants were grown in seven liter pots filled with sandy substrate irrigated daily with a nutritive solution. The treatments were represented by timing of sampling, performed at 14 day intervals, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162 and 176 days after emergence (DAE) of R. brasiliensis plants; and 21, 35, 49, 63, 77, 91, 105 and 119 DAE of the soybean plants BR-16 (early maturity) For both plants, the leaves had the highest biomass partition during seven weeks. For these data, partition was higher in reproductive structures in soybean and in the stems for Brazilian pusley. The maximumcritical biomass accumulation was estimated at 104 days for soybean (36.6 g plant-1) and at 146 days for R. brasiliensis (16.4 g planta-1). From emergence up to 50 DAE, the leaves presented higher participation in biomass accumulation, in both species. After 50 DAE, it was observed in both species an inversion in leaf representativity per shoots, for the weed species and per shoots and later per reproductive structures, for thecrop. The maximumdaily macronutrient uptake ratewas achieved between 69 and 87 days in soybean and 106 and 111 DAE in R. brasiliensis. Considering of the inflexion point average observed for the soybean culture, it is possible to estimate that, at 78 DAE, this plant theoretically accumulates 25.9 g of dry weight ; 615.6 mg oN; 77.2 mg P; 538.6 mg K; 535.0 mg Ca; 171.5 mg Mg and 39,5 mg S. In the same period, one razilian pusley plant accumulates 3.7 g dry weight; 50.8 mg N; 3.2 mg P; 104.4 mg K; 127.8 mg Ca; 18.8 mg Mg and 3. 7 mg oS. Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à Agropecuária Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à Agropecuária

Embora o herbicida glyphosate seja considerado não-seletivo, várias espécies de plantas daninhas apresentam certo grau de tolerância às doses recomendadas. No Brasil, já existem relatos de seleção de espécies de plantas daninhas tolerantes ao glyphosate em áreas com o uso intensivo deste herbicida. Assim, o objetivo desta pesquisa foi avaliar a influência da aplicação repetitiva do herbicida glyphosate sobre a dinâmica do banco de sementes das plantas daninhas tolerantes: Commelina benghalensis, Ipomoea grandifolia e Richardia brasiliensis; e das plantas daninhas suscetíveis: Amaranthus hybridus e Galinsoga parviflora. O experimento foi monitorado durante dois anos em áreas experimentais do Departamento de Produção Vegetal da Escola Superior de Agricultura ''Luiz de Queiroz'' - Universidade de São Paulo, Piracicaba, São Paulo. Partindo de uma densidade de 100 plantas m-2 de cada espécie, o glyphosate foi aplicado periodicamente em pós-emergência sobre as plantas daninhas adultas nas doses de 0, 0,36, 1,44 e 2,88 kg e.a. ha-1. O banco de sementes do solo foi estimado através da avaliação de emergência direta das plântulas e extração física das sementes. O banco de sementes das plantas daninhas tolerantes ao glyphosate teve aumento significativo, principalmente nas menores doses. Por outro lado, o banco de sementes das espécies suscetíveis diminuiu ao longo do tempo mesmo nas menores doses do glyphosate. Na última análise do banco de sementes, as plantas daninhas tolerantes apresentaram, na maior dose de glyphosate aplicada, cerca de 12 milhões de sementes não-dormentes ha-1, ao passo que as suscetíveis apresentaram cerca de 4 milhões de sementes não-dormentes ha-1. Concluiu-se que o uso intensivo do herbicida glyphosate pode ocasionar uma mudança na área, aumentando a freqüência das plantas tolerantes ao longo dos anos. Although glyphosate is considered a non- selective herbicide, several weed species show a certain degree of tolerance to the recommended rates. Weed species tolerance to glyphosate in areas with intensive application of this herbicide has been reported in Brazil. Thus, the objective of this research was to evaluate the influence of the herbicide glyphosate on the seedbank dynamics of the tolerant weeds Commelina benghalensis, Ipomoea grandifolia and Richardia brasiliensis; and the susceptible weeds Amaranthus hybridus and Galinsoga parviflora. The experiment was monitored during two years in the experimental area of Department of Plant Production of ESALQ/University of São Paulo, Brazil. Beginning with an initial density of 100 plants m-2 of each species, glyphosate was sprayed periodically under post-emergence conditions at the rates of 0, 0.36, 1.44 and 2.88 kg a.e/ha. The seedbank in the soil was estimated through seedling emergence evaluation and physical extraction from the soil. Seedbank of the tolerant species increased specially at lower rates of glyphosate. On the other hand, the seedbank of susceptible species decreased during the experiment, even at lower rates. In the last seedbank evaluation, the tolerant weeds presented almost 12 million of seeds/ha, while the susceptible weeds showed about four million seeds/ha. It was concluded that the intensive use of glyphosate can cause a weed shift in the area, increasing the frequency of tolerant weeds along the years.

Richardia brasiliensis (Rubiaceae), also known as white eye or ‘poaia-branca’ in Brazil, is an important agricultural weed in the tropics (2). Relatively little is known about diseases affecting this species. In March 2013, all of the plants of this weed species invading an orchid plantation in Nova Friburgo (State of Rio de Janeiro) and a private orchard at Viçosa (State of Minas Gerais) in Brazil were found to bear intense leaf blight symptoms. Lesions were circular to elliptical, 1.4 to 10.5 mm in diameter, grayish to pale brown, and coalesced leading to necrosis of large areas of the leaves. Leaf samples were collected, dried in a plant press, and representative specimens deposited in the local herbarium at the Universidade Federal de Viçosa (Accession Nos. VIC 39759 and VIC 39760). A fungus found in association with diseased tissues was isolated by directly transferring conidia from infected leaves onto PDA plates, and two isolates were deposited in a local culture collection (COAD Accession Nos. 1335 and 1443). Conidia were removed from infected leaves using a scalpel, and mounted in lactophenol and lactofuchsin for observation with a light microscope (Olympus BX 51). Conidiophores were epiphylous, isolated, subcylindrical, straight to slightly curved, 97.5 to 170.0 × 5.0 to 8.0 μm, 2 to 6 septate, unbranched, pale brown and paler towards the apex, and smooth. Conidia were straight to slightly curved, pyriform to obovoid, 35.5 to 43.5 × 12.5 to 25.0 μm, with the apex rounded and the base subacute, 1 to 3 distoseptate, the subterminal cell often dark brown and larger than the other cells (sometimes leading to the distortion and curving of conidia); the other cells were golden brown and the conidia were smooth. The morphology of the fungus on R. brasiliensis was equivalent to that described for Curvularia richardiae (1). Genomic DNA was extracted from a 7-day-old pure culture of both isolates, and the large subunit (LSU) region of ribosomal DNA (rDNA) was amplified with the primers LR0R/LR5 (3). The resulting sequences were deposited in GenBank (KF880800 and KF880801). A BLASTn search revealed 99% similarity of the two isolates from Brazil with the LSU sequence of an isolate of Cochiobolus geniculatus (JN941528). Three healthy, 10-cm-tall R. brasiliensis plants were inoculated with a conidial suspension (1 × 106 conidia/ml) of isolate COAD 1335 until runoff, and the plants kept for 2 days in a dew chamber at 26 ± 3°C. Additionally, two plants were sprayed with distilled water and kept under the same conditions. Six days after inoculation, symptoms appeared on all inoculated plants that were similar to symptoms on plants in the field. Non-treated control plants remained healthy. C. richardiae was isolated from the lesions on inoculated plants. Although there is an incomplete record of a Curvularia sp. associated with seeds of R. brasiliensis in Brazil (4), that record included no description of the fungus or information on a disease caused on the plants. This is the first report of C. richardiae causing a disease on R. brasiliensis in Brazil. Although the fungus was first described in Australia (1), C. richardiae is most likely a native from the neotropics, as is the host plant, R. brasiliensis. The fungus was probably introduced accidentally into Australia on the weedy host but has remained unnoticed in the native range until now. References: (1) J. L. Alcorn. Trans. Brit. Mycol. Soc. 56:155, 1971. (2) R. R. Rosseto et al. Planta Daninha 15:25, 1997. (3) R. Vilgalys et al. J. Bacteriol. 172:4239, 1990. (4) C. Yamashita et al. Fitopatol. Bras. 13:122, 1988.

The antifungal properties of ethanol and water crude extracts of the bark of Brachystegia eurycoma and leaf of Richaria brasiliensis were analysed using disc diffisopm agar method. After 48 hours of incubation, all the four extracts at 2mg/ml inhibited the growth of Aspergillus flavus. Aspergillus fumigatus, Aspergillus niger, Candida albicans, Epidermophyton floccosium, Fusarium solani, Mucor mucedo, Microsporium audonii and Trichophyton verrucosum. The ethanol extract of Richardia brasiliensis was the most active of the four extrtacts. The water extract of Brachystegia eurycoma was more active than its ethanol extract, but the dreverse occurred with Richardia brasiliensis because the ethanol extract was more active than its water extract. Preliminary phytochemical studies of the plant extracts reveals that Richardia brasiliensis water extract contains mainly tannin while its ethanol extract possess antraquinone, flavonoid, pholbatannin, saponins and steroids. The water extract of Brachystegia eurycoma has tannin mainly while its ethanol extract has flavonoid and tannin. Alkaloid and terpenes were absent in the four plant extracts. Key Words: Antifungal, Brachystegia eurycoma, Caesalphinaceae, Richardia brasiliensis, Ruboaceae, Tannin. Nig. J. Nat Prod. And Med. Vol.4 2000: 70-72

O presente experimento, inteiramente casualizado, foi desenvolvido em condições de laboratório no Departamento de Defesa Fitossanitária, FCA/UNESP - Botucatu, entre julho e setembro de 1992. Amostras de Areia Quartzosa equivalentes à 40 g de terra seca à 105 oC ± 2 com ou sem adição de 1,9 g de matéria seca de plantas de poaia-branca (Richardia brasiliensis), 0,19 g de nitrogênio (NH4)2SO4 e 0,88 g de apatita de Araxá, foram incubadas no escuro a 25 o C ± 2 , com umidade mantida a 60% da capacidade de retenção de água. Durante a incubação, determinou-se o CO2 liberado, utilizando-se o método de retenção em NAOH seguida de titulometria com HCl; a biomassa microbiana, método de fumigação-incubação; o pH e a quantidade de fósforo extraído por resina. A maior liberação de CO2 ocorreu durante os dez primeiros dias de incubação, com 77% do total de carbono liberado nos tratamentos com adição de poaia, e 37% nos tratamentos sem adição da mesma. A liberação de CO2 foi 57 vezes maior nos tratamentos com poaia em relação ao controle. A poaia também provocou aumentos na biomassa microbiana (média de 8 vezes a biomassa do tratamento controle), e a adição de nitrogênio e/ou fosfato de rocha junto à poaia antecipou os picos de formação de biomassa de 20 para 10 dias de incubação. Os níveis de fósforo disponível foram maiores no tratamento com adição de fosfato de rocha apenas. A poaia também alcalinizou o sistema, não permitindo desta forma, observar-se relação significativa entre pH e teor de fósforo disponível. The present experiment, totally randomized, was carried out under laboratory conditions at the Departamento de Defesa Fitossanitária, FCA/UNESP-Botucatu, between July and September, 1992. Fresh air-dry samples of quartz sand representing 40g of dry soil each, at 105 o C ± 2, with or without addition of 1.90g of dry matter of "poaia-branca" (Richardia brasiliensis) plants, 0.19g of nitrogen (NH4)2SO4 and 0.88g of Araxá apatite, were incubated under dark conditions at 25 oC ±2,with moisture held at 60% water retention capacity. During incubation, CO2-liberation was determined by means of the method of retention in NaOH followed by titration with HCl; microbial biomass was determined through the fumigation-incubation method, while pH and phosphorus extract by resin. CO2-liberation was high during the first ten days of incubation, with 77% of total carbon released in treatments with "poaia" addition, and 37% in treatments without addition. CO2-liberation was 57 times higher in the "Poaia" treatments compared to the control.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:51:20Z No. of bitstreams: 1 S0100-83581999000100007.pdf: 175533 bytes, checksum: 31443c875797b5f9292ea245f6fee7a0 (MD5) Made available in DSpace on 2013-08-22T18:51:21Z (GMT). No. of bitstreams: 1 S0100-83581999000100007.pdf: 175533 bytes, checksum: 31443c875797b5f9292ea245f6fee7a0 (MD5) Previous issue date: 1999-04-01 Made available in DSpace on 2013-09-30T17:48:15Z (GMT). No. of bitstreams: 2 S0100-83581999000100007.pdf: 175533 bytes, checksum: 31443c875797b5f9292ea245f6fee7a0 (MD5) S0100-83581999000100007.pdf.txt: 27734 bytes, checksum: 75f45d270e92865b8cfcba5b990e9924 (MD5) Previous issue date: 1999-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:21:21Z No. of bitstreams: 2 S0100-83581999000100007.pdf: 175533 bytes, checksum: 31443c875797b5f9292ea245f6fee7a0 (MD5) S0100-83581999000100007.pdf.txt: 27734 bytes, checksum: 75f45d270e92865b8cfcba5b990e9924 (MD5) Made available in DSpace on 2014-05-20T13:21:21Z (GMT). No. of bitstreams: 2 S0100-83581999000100007.pdf: 175533 bytes, checksum: 31443c875797b5f9292ea245f6fee7a0 (MD5) S0100-83581999000100007.pdf.txt: 27734 bytes, checksum: 75f45d270e92865b8cfcba5b990e9924 (MD5) Previous issue date: 1999-04-01 O presente experimento, inteiramente casualizado, foi desenvolvido em condições de laboratório no Departamento de Defesa Fitossanitária, FCA/UNESP - Botucatu, entre julho e setembro de 1992. Amostras de Areia Quartzosa equivalentes à 40 g de terra seca à 105 oC ± 2 com ou sem adição de 1,9 g de matéria seca de plantas de poaia-branca (Richardia brasiliensis), 0,19 g de nitrogênio (NH4)2SO4 e 0,88 g de apatita de Araxá, foram incubadas no escuro a 25 o C ± 2 , com umidade mantida a 60% da capacidade de retenção de água. Durante a incubação, determinou-se o CO2 liberado, utilizando-se o método de retenção em NAOH seguida de titulometria com HCl; a biomassa microbiana, método de fumigação-incubação; o pH e a quantidade de fósforo extraído por resina. A maior liberação de CO2 ocorreu durante os dez primeiros dias de incubação, com 77% do total de carbono liberado nos tratamentos com adição de poaia, e 37% nos tratamentos sem adição da mesma. A liberação de CO2 foi 57 vezes maior nos tratamentos com poaia em relação ao controle. A poaia também provocou aumentos na biomassa microbiana (média de 8 vezes a biomassa do tratamento controle), e a adição de nitrogênio e/ou fosfato de rocha junto à poaia antecipou os picos de formação de biomassa de 20 para 10 dias de incubação. Os níveis de fósforo disponível foram maiores no tratamento com adição de fosfato de rocha apenas. A poaia também alcalinizou o sistema, não permitindo desta forma, observar-se relação significativa entre pH e teor de fósforo disponível. The present experiment, totally randomized, was carried out under laboratory conditions at the Departamento de Defesa Fitossanitária, FCA/UNESP-Botucatu, between July and September, 1992. Fresh air-dry samples of quartz sand representing 40g of dry soil each, at 105 o C ± 2, with or without addition of 1.90g of dry matter of poaia-branca (Richardia brasiliensis) plants, 0.19g of nitrogen (NH4)2SO4 and 0.88g of Araxá apatite, were incubated under dark conditions at 25 oC ±2,with moisture held at 60% water retention capacity. During incubation, CO2-liberation was determined by means of the method of retention in NaOH followed by titration with HCl; microbial biomass was determined through the fumigation-incubation method, while pH and phosphorus extract by resin. CO2-liberation was high during the first ten days of incubation, with 77% of total carbon released in treatments with poaia addition, and 37% in treatments without addition. CO2-liberation was 57 times higher in the Poaia treatments compared to the control. UNESP FCA Depto de Defesa Fitossanitária UNESP FCA Deptartamento de Defesa Fitossanitária UNESP FCA Departamento de Ciência do Solo UNESP FCA Depto de Defesa Fitossanitária UNESP FCA Deptartamento de Defesa Fitossanitária UNESP FCA Departamento de Ciência do Solo