Search

Your search keyword '"Richardson, Hollian"' showing total 96 results
Start Over Author "Richardson, Hollian"
96 results on '"Richardson, Hollian"'

1. Dipeptidyl peptidase-1 inhibition in patients hospitalised with COVID-19: a multicentre, double-blind, randomised, parallel-group, placebo-controlled trial

Author

Chalmers, James, Abo-Leyah, Hani, New, Benjamin JM, Almaden-Boyle, Christine, Connell, David, Taylor, Jennifer, Strachan, Jodie, Loftus, Heather, Young, Lesley, Strachan, Angela, Band, Margaret, McLaren-Neil, Fiona, Pilvinyte, Kristina, Adamson, Simon, Lahnsteiner, Eva, Rauchhaus, Petra, Hogarth, Fiona, George, Jacob, Burns, Tricia, Coote, Elizabeth, Keiller, Marney, Patel, Manish, Smith, Andrew, Sage, Elizabeth, Cooper, Jamie, Miller, David, Dosanjh, Davinder, Sutton, Benjamin, Underwood, Jonathan, Frayling, Sharon, Haynes, Matthew, Broad, Lauren, Jones, Laura, Rahilly, Karen, Oliver, Catherine, Evans, Terriann, Balan, Andrea, Davies, Rhys, Forde, Donal, Nye, Clemency, Haboubi, Dr, Hilton, Zoe, Williams, Jennie, McQueen, Alison, Spears, Mark, Edmond, Ian, Salutous, Dario, McGenily, Laura, Scott, Rhona, Henderson, Eilidh, Collins, Andrea, Dhasmana, Devesh, Liu, Patrick, Morrow, Ana, Couser, Mandy, Davey, Fleur, Hicks, Alexander, Wiffen, Laura, Fox, Lauren, Abdelrahim, Mohamed, Darbyshire, Alexander, Cowen, Elena, Rowley, Megan, Giles, Benjamin, Yang, Yingjia, Brown, Tom, Rupani, Hitasha, Hawes, Elizabeth, Barnes, Debi, Brogan, Fiona, Bungue-Tuble, Roneleeh, Howe, Serena, Turner, Charlotte, Baryschpolec, Sonia, Longhurst, Bev, Moon, Maria, Watkins, Lynn, Baker-Moffat, Michelle, Murray, Lisa, Harrington-Davies, Yasmin, Burrows, Kate, Minnis, Chrissie, Wands, Mary, Bamgboye, Adefunke, Wong, Charlotte, Brightling, Christopher, Diver, Sarah, Russell, Richard, McAuley, Hamish, Elneima, Omer, Yousuf, Ahmed, McCourt, Paula, Hargadon, Beverley, Parker, Sarah, Bourne, Michelle, Suntharalingam, Jay, Hartley, Tom, Masan, Vidan, Sturney, Sharon, MacKenzie, Rob, Marchand, Clare, Mason, Rebecca, White, Katie, Kirby, Alison, Meda, Manjula, Diwakar, Lavanya, Russell, Peter, Finn, Joanne, Harris, Sophie, Muir, Carol, Cook, Gemma, Staines, Nikki, Cook, Chris, Thompson, AA Roger, Condliffe, Alison, Hull, Rebecca, Dowey, Rebecca, Turton, Helena, Collini, Paul, Gabriel, Zoé, Hardman, Simon, Newell, Helen, Middle, Janet, Simpson, Phillip, Colton, Hayley, Barker, Joann, Birchall, Katie, Harrington, Kate, Housley, Kay, Lenagh, Rebecca, Wilson, Jayne, Wesonga, Joan, Whitham, Rachel, Bird, Sarah, Jackson, Yvonne, Mbuyisa, Angeline, Anderson, Samantha, Wilson, Anna, Kibutu, Faith, Walker, Sara, Cawthron, Kay, Macharia, Irene, Smart, Lynne, Emery, Anna, Howell, Alice, Hurditch, Elizabeth, Ford, Amber, Turner, Kim, Watson, Lisa, Bowler, Helen, Jackson, Tracy, Jaques, Carol, Dyer, Nichole, Ducker, Shelley, Goodall, Vicky, Udale, Emily, Keir, Holly R, Long, Merete B, Giam, Yan Hui, Vadiveloo, Thenmalar, Pembridge, Thomas, Hull, Rebecca C, Delgado, Lilia, Gilmour, Amy, Hughes, Chloe, Richardson, Hollian, Cassidy, Diane, Dosanjh, Davinder PS, Sage, Beth, Condliffe, Alison M, Shoemark, Amelia, MacLennan, Graeme, and Chalmers, James D

Published
2022
Full Text
View/download PDF

2. Airway IL-1β is related to disease severity and mucociliary function in bronchiectasis

Author

Perea, Lidia, primary, Bottier, Mathieu, additional, Cant, Erin, additional, Richardson, Hollian, additional, Dicker, Alison J, additional, Shuttleworth, Morven, additional, Giam, Yan Hui, additional, Abo-Leyah, Hani, additional, Finch, Simon, additional, Huang, Jeffrey T-J, additional, Shteinberg, Michal, additional, Goeminne, Pieter C, additional, Polverino, Eva, additional, Altenburg, Josje, additional, Blasi, Francesco, additional, Welte, Tobias, additional, Aliberti, Stefano, additional, Sibila, Oriol, additional, Chalmers, James D, additional, and Shoemark, Amelia, additional

Published
2024
Full Text
View/download PDF

3. Endotypes of Exacerbation in Bronchiectasis: An Observational Cohort Study

Author

Gao, Yonghua, primary, Richardson, Hollian, additional, Dicker, Alison J., additional, Barton, Alun, additional, Kuzmanova, Elena, additional, Shteinberg, Michal, additional, Perea, Lidia, additional, Goeminne, Pieter C., additional, Cant, Erin, additional, Hennayake, Chandani, additional, Pollock, Jennifer, additional, Abo Leyah, Hani, additional, Choi, Hayoung, additional, Polverino, Eva, additional, Blasi, Francesco, additional, Welte, Tobias, additional, Aliberti, Stefano, additional, Long, Merete, additional, Shoemark, Amelia, additional, Sibila, Oriol, additional, Huang, Jeffrey T. J., additional, and Chalmers, James D., additional

Published
2024
Full Text
View/download PDF

4. Airway "Resistotypes" and Clinical Outcomes in Bronchiectasis.

Author

Mac Aogáin, Micheál, Xaverius Ivan, Fransiskus, Jaggi, Tavleen Kaur, Richardson, Hollian, Shoemark, Amelia, Narayana, Jayanth Kumar, Dicker, Alison J., Koh, Mariko Siyue, Lee, Ken Cheah Hooi, Thun How, Ong, Poh, Mau Ern, Chin, Ka Kiat, Hou, Albert Lim Yick, Ser Hon, Puah, Low, Teck Boon, Abisheganaden, John Arputhan, Dimakou, Katerina, Digalaki, Antonia, Kosti, Chrysavgi, and Gkousiou, Anna

Subjects
BRONCHIECTASIS, TREATMENT effectiveness, MULTIDRUG resistance, AIRWAY (Anatomy), DISEASE exacerbation, DRUG resistance in microorganisms
Abstract

Rationale: Chronic infection and inflammation shapes the airway microbiome in bronchiectasis. Utilizing whole-genome shotgun metagenomics to analyze the airway resistome provides insight into interplay between microbes, resistance genes, and clinical outcomes. Objectives: To apply whole-genome shotgun metagenomics to the airway microbiome in bronchiectasis to highlight a diverse pool of antimicrobial resistance genes: the "resistome," the clinical significance of which remains unclear. Methods: Individuals with bronchiectasis were prospectively recruited into cross-sectional and longitudinal cohorts (n = 280), including the international multicenter cross-sectional Cohort of Asian and Matched European Bronchiectasis 2 (CAMEB 2) study (n = 251) and two independent cohorts, one describing patients experiencing acute exacerbation and a further cohort of patients undergoing Pseudomonas aeruginosa eradication treatment. Sputum was subjected to metagenomic sequencing, and the bronchiectasis resistome was evaluated in association with clinical outcomes and underlying host microbiomes. Measurements and Main Results: The bronchiectasis resistome features a unique resistance gene profile and increased counts of aminoglycoside, bicyclomycin, phenicol, triclosan, and multidrug resistance genes. Longitudinally, it exhibits within-patient stability over time and during exacerbations despite between-patient heterogeneity. Proportional differences in baseline resistome profiles, including increased macrolide and multidrug resistance genes, associate with shorter intervals to the next exacerbation, whereas distinct resistome archetypes associate with frequent exacerbations, poorer lung function, geographic origin, and the host microbiome. Unsupervised analysis of resistome profiles identified two clinically relevant "resistotypes," RT1 and RT2, the latter characterized by poor clinical outcomes, increased multidrug resistance, and P. aeruginosa. Successful targeted eradication in P. aeruginosa–colonized individuals mediated reversion from RT2 to RT1, a more clinically favorable resistome profile demonstrating reduced resistance gene diversity. Conclusions: The bronchiectasis resistome associates with clinical outcomes, geographic origin, and the underlying host microbiome. Bronchiectasis resistotypes link to clinical disease and are modifiable through targeted antimicrobial therapy. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

5. Sputum metagenomics of people with bronchiectasis

Author

Rosenboom, Ilona, primary, Thavarasa, Ajith, additional, Richardson, Hollian, additional, Long, Merete B., additional, Wiehlmann, Lutz, additional, Davenport, Colin F., additional, Shoemark, Amelia, additional, Chalmers, James D., additional, and Tümmler, Burkhard, additional

Published
2024
Full Text
View/download PDF

6. Bronchiectasis with Chronic Rhinosinusitis Is Associated with Eosinophilic Airway Inflammation and Is Distinct from Asthma

Author

Shteinberg, Michal, primary, Chalmers, James D., additional, Narayana, Jayanth K., additional, Dicker, Alison J., additional, Rahat, Michal A, additional, Simanovitch, Elina, additional, Bidgood, Lucy, additional, Cohen, Shai, additional, Stein, Nili, additional, Abo-Hilu, Nizar, additional, Abbott, James, additional, Avital, Sharon, additional, Fireman-Klein, Einat, additional, Richardson, Hollian, additional, Muhammad, Emad, additional, Jrbashyan, Jenny, additional, Schneer, Sonia, additional, Nasrallah, Najwan, additional, Eisenberg, Iya, additional, Chotirmall, Sanjay H, additional, and Adir, Yochai, additional

Published
2024
Full Text
View/download PDF

7. Inflammatory Molecular Endotypes in Bronchiectasis: A European Multicenter Cohort Study

Author

Choi, Hayoung, primary, Ryu, Soorack, additional, Keir, Holly R, additional, Giam, Yan Hui, additional, Dicker, Alison J., additional, Perea, Lidia, additional, Richardson, Hollian, additional, Huang, Jeffrey T. J., additional, Cant, Erin, additional, Blasi, Francesco, additional, Pollock, Jennifer, additional, Shteinberg, Michal, additional, Finch, Simon, additional, Aliberti, Stefano, additional, Sibila, Oriol, additional, Shoemark, Amelia, additional, and Chalmers, James D., additional

Published
2023
Full Text
View/download PDF

8. The airway microbiome in Bronchiectasis-COPD overlap: data from the EMBARC-BRIDGE study

Author

Keir, Holly R., primary, Dicker, Alison J., additional, Richardson, Hollian, additional, Kewin, Ellie, additional, Perea Soriano, Lidia, additional, Pollock, Jennifer, additional, Giam, Yan H, additional, Crichton, Megan, additional, Choi, Hayoung, additional, Cant, Erin, additional, Finch, Simon, additional, Fong, Christopher J., additional, Blasi, Francesco, additional, Shoemark, Amelia, additional, Sibila, Oriol, additional, Aliberti, Stefano, additional, and Chalmers, James D, additional

Published
2023
Full Text
View/download PDF

10. The effect of different inhaled corticosteroids on airway bacterial load and the microbiome in COPD: a randomized controlled trial

Author

Richardson, Hollian, primary, Keir, Holly R, additional, Clarke, Clare, additional, Alferes De Lima, Daniela, additional, Veluchamy, Abirami, additional, Rauchhaus, Petra, additional, Pembridge, Thomas, additional, Crichton, Megan, additional, Pollock, Jennifer, additional, Finch, Simon, additional, Dicker, Alison J, additional, Billingham, Pauline, additional, Hussain, Furrah, additional, Band, Margaret, additional, Smith, Andrew, additional, Patel, Manish, additional, Paracha, Mohammad, additional, Choudhury, Gourab, additional, Dhasmana, Devesh, additional, Chaudhuri, Rekha, additional, Short, Philip M, additional, and Chalmers, James D, additional

Published
2023
Full Text
View/download PDF

11. Inflammatory Endotypes, Microbiome and Exacerbation Risk in Bronchiectasis: A European Multicentre Study

Author

Choi, Hayoung, primary, Ryu, Soorack, additional, Keir, Holly, additional, Giam, Yan Hui, additional, Dicker, Alison, additional, Perea, Lidia, additional, Richardson, Hollian, additional, Huang, Jeffrey, additional, Cant, Erin, additional, Blasi, Francesco, additional, Pollock, Jennifer, additional, Shteinberg, Michal, additional, Finch, Simon, additional, Aliberti, Stefano, additional, Sibila, Oriol, additional, Shoemark, Amelia, additional, and Chalmers, James, additional

Published
2023
Full Text
View/download PDF

13. Inflammatory Molecular Endotypes in Bronchiectasis: A European Multicenter Cohort Study.

Author

Hayoung Choi, Soorack Ryu, Keir, Holly R., Yan Hui Giam, Dicker, Alison J., Perea, Lidia, Richardson, Hollian, Huang, Jeffrey T. J., Cant, Erin, Blasi, Francesco, Pollock, Jennifer, Shteinberg, Michal, Finch, Simon, Aliberti, Stefano, Sibila, Oriol, Shoemark, Amelia, and Chalmers, James D.

Subjects
BRONCHIECTASIS, COHORT analysis, RIBOSOMAL RNA, PHYLA (Genus), RNA sequencing, CLUSTER analysis (Statistics)
Abstract

Rationale: Although inflammation and infection are key disease drivers in bronchiectasis, few studies have integrated host inflammatory and microbiome data to guide precision medicine. Objectives: To identify clusters among patients with bronchiectasis on the basis of inflammatory markers and to assess the association between inflammatory endotypes, microbiome characteristics, and exacerbation risk. Methods: Patients with stable bronchiectasis were enrolled at three European centers, and cluster analysis was used to stratify the patients according to the levels of 33 sputum and serum inflammatory markers. Clusters were compared in terms of microbiome composition (16S ribosomal RNA sequencing) and exacerbation risk over a 12-month follow-up. Measurements and Main Results: A total of 199 patients were enrolled (109 [54.8%] female; median age, 69 yr). Four clusters of patients were defined according to their inflammatory profiles: cluster 1, milder neutrophilic inflammation; cluster 2, mixedneutrophilic and type 2; cluster 3, most severe neutrophilic; and cluster 4, mixed-epithelial and type 2. Lower microbiome diversity was associated with more severe inflammatory clusters (P,0.001), and b-diversity analysis demonstrated distinct microbiome profiles associated with each inflammatory cluster (P = 0.001). Proteobacteria and Pseudomonas at phylum and genus levels, respectively, were more enriched in clusters 2 and 3 than in clusters 1 and 4. Furthermore, patients in cluster 2 (rate ratio [RR], 1.49; 95% confidence interval [CI], 1.16-1.92) and cluster 3 (RR, 1.61; 95% CI, 1.12-2.32) were at higher risk of exacerbation over a 12-month follow-up compared with cluster 1, even after adjustment for prior exacerbation history. Conclusions: Bronchiectasis inflammatory endotypes are associated with distinct microbiome profiles and future exacerbation risk. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

14. Dipeptidyl peptidase-1 inhibition in patients hospitalised with COVID-19: a multicentre, double-blind, randomised, parallel-group, placebo-controlled trial

Author

Keir, Holly R, primary, Long, Merete B, additional, Abo-Leyah, Hani, additional, Giam, Yan Hui, additional, Vadiveloo, Thenmalar, additional, Pembridge, Thomas, additional, Hull, Rebecca C, additional, Delgado, Lilia, additional, Band, Margaret, additional, McLaren-Neil, Fiona, additional, Adamson, Simon, additional, Lahnsteiner, Eva, additional, Gilmour, Amy, additional, Hughes, Chloe, additional, New, Benjamin JM, additional, Connell, David, additional, Dowey, Rebecca, additional, Turton, Helena, additional, Richardson, Hollian, additional, Cassidy, Diane, additional, Cooper, Jamie, additional, Suntharalingam, Jay, additional, Diwakar, Lavanya, additional, Russell, Peter, additional, Underwood, Jonathan, additional, Hicks, Alexander, additional, Dosanjh, Davinder PS, additional, Sage, Beth, additional, Dhasmana, Devesh, additional, Spears, Mark, additional, Thompson, AA Roger, additional, Brightling, Christopher, additional, Smith, Andrew, additional, Patel, Manish, additional, George, Jacob, additional, Condliffe, Alison M, additional, Shoemark, Amelia, additional, MacLennan, Graeme, additional, Chalmers, James D, additional, Chalmers, James, additional, Almaden-Boyle, Christine, additional, Taylor, Jennifer, additional, Strachan, Jodie, additional, Loftus, Heather, additional, Young, Lesley, additional, Strachan, Angela, additional, Pilvinyte, Kristina, additional, Rauchhaus, Petra, additional, Hogarth, Fiona, additional, Burns, Tricia, additional, Coote, Elizabeth, additional, Keiller, Marney, additional, Sage, Elizabeth, additional, Miller, David, additional, Dosanjh, Davinder, additional, Sutton, Benjamin, additional, Frayling, Sharon, additional, Haynes, Matthew, additional, Broad, Lauren, additional, Jones, Laura, additional, Rahilly, Karen, additional, Oliver, Catherine, additional, Evans, Terriann, additional, Balan, Andrea, additional, Davies, Rhys, additional, Forde, Donal, additional, Nye, Clemency, additional, Haboubi, Dr, additional, Hilton, Zoe, additional, Williams, Jennie, additional, McQueen, Alison, additional, Edmond, Ian, additional, Salutous, Dario, additional, McGenily, Laura, additional, Scott, Rhona, additional, Henderson, Eilidh, additional, Collins, Andrea, additional, Liu, Patrick, additional, Morrow, Ana, additional, Couser, Mandy, additional, Davey, Fleur, additional, Wiffen, Laura, additional, Fox, Lauren, additional, Abdelrahim, Mohamed, additional, Darbyshire, Alexander, additional, Cowen, Elena, additional, Rowley, Megan, additional, Giles, Benjamin, additional, Yang, Yingjia, additional, Brown, Tom, additional, Rupani, Hitasha, additional, Hawes, Elizabeth, additional, Barnes, Debi, additional, Brogan, Fiona, additional, Bungue-Tuble, Roneleeh, additional, Howe, Serena, additional, Turner, Charlotte, additional, Baryschpolec, Sonia, additional, Longhurst, Bev, additional, Moon, Maria, additional, Watkins, Lynn, additional, Baker-Moffat, Michelle, additional, Murray, Lisa, additional, Harrington-Davies, Yasmin, additional, Burrows, Kate, additional, Minnis, Chrissie, additional, Wands, Mary, additional, Bamgboye, Adefunke, additional, Wong, Charlotte, additional, Diver, Sarah, additional, Russell, Richard, additional, McAuley, Hamish, additional, Elneima, Omer, additional, Yousuf, Ahmed, additional, McCourt, Paula, additional, Hargadon, Beverley, additional, Parker, Sarah, additional, Bourne, Michelle, additional, Hartley, Tom, additional, Masan, Vidan, additional, Sturney, Sharon, additional, MacKenzie, Rob, additional, Marchand, Clare, additional, Mason, Rebecca, additional, White, Katie, additional, Kirby, Alison, additional, Meda, Manjula, additional, Finn, Joanne, additional, Harris, Sophie, additional, Muir, Carol, additional, Cook, Gemma, additional, Staines, Nikki, additional, Cook, Chris, additional, Condliffe, Alison, additional, Hull, Rebecca, additional, Collini, Paul, additional, Gabriel, Zoé, additional, Hardman, Simon, additional, Newell, Helen, additional, Middle, Janet, additional, Simpson, Phillip, additional, Colton, Hayley, additional, Barker, Joann, additional, Birchall, Katie, additional, Harrington, Kate, additional, Housley, Kay, additional, Lenagh, Rebecca, additional, Wilson, Jayne, additional, Wesonga, Joan, additional, Whitham, Rachel, additional, Bird, Sarah, additional, Jackson, Yvonne, additional, Mbuyisa, Angeline, additional, Anderson, Samantha, additional, Wilson, Anna, additional, Kibutu, Faith, additional, Walker, Sara, additional, Cawthron, Kay, additional, Macharia, Irene, additional, Smart, Lynne, additional, Emery, Anna, additional, Howell, Alice, additional, Hurditch, Elizabeth, additional, Ford, Amber, additional, Turner, Kim, additional, Watson, Lisa, additional, Bowler, Helen, additional, Jackson, Tracy, additional, Jaques, Carol, additional, Dyer, Nichole, additional, Ducker, Shelley, additional, Goodall, Vicky, additional, and Udale, Emily, additional

Published
2022
Full Text
View/download PDF

15. Dipeptidyl Peptidase-1 Inhibition in Patients Hospitalized with COVID-19: a Multicentre Randomized Double-Blind Placebo Controlled Trial

Author

Keir, Holly R., primary, Long, Merete B., additional, Abo-Leyah, Hani, additional, Giam, Yan H., additional, Vadiveloo, Thenmalar, additional, Pembridge, Thomas, additional, Hull, Rebecca C., additional, Delgado, Lilia, additional, Band, Margaret, additional, Mclaren-Neil, Fiona, additional, Adamson, Simon, additional, Lahnsteiner, Eva, additional, Gilmour, Amy, additional, Hughes, Chloe, additional, New, Benjamin Jm., additional, Connell, David, additional, Dowey, Rebecca, additional, Turton, Helena, additional, Richardson, Hollian, additional, Cassidy, Diane, additional, Cooper, Jamie, additional, Suntharalingam, Jay, additional, Diwakar, Lavanya, additional, Russell, Peter, additional, Underwood, Jonathan, additional, Hicks, Alexander, additional, Dosanjh, Davinder Ps, additional, Sage, Beth, additional, Dhasmana, Devesh, additional, Spears, Mark, additional, Thompson, Aa Roger, additional, Brightling, Christopher, additional, Smith, Andrew, additional, Patel, Manish, additional, George, Jacob, additional, Condliffe, Alison M, additional, Shoemark, Amelia, additional, Maclennan, Graeme, additional, and Chalmers, James D, additional

Published
2022
Full Text
View/download PDF

16. Neutrophil proteomics identifies temporal changes and hallmarks of delayed recovery in COVID19

Author

Long, Merete B, primary, Howden, Andrew JM, additional, Keir, Holly R, additional, Rollings, Christina M, additional, Giam, Yan Hui, additional, Pembridge, Thomas, additional, Delgado, Lilia, additional, Abo-Leyah, Hani, additional, Lloyd, Amy F, additional, Sollberger, Gabriel, additional, Hull, Rebecca, additional, Gilmour, Amy, additional, Hughes, Chloe, additional, New, Benjamin JM, additional, Cassidy, Diane, additional, Shoemark, Amelia, additional, Richardson, Hollian, additional, Lamond, Angus I, additional, Cantrell, Doreen A, additional, Chalmers, James D, additional, and Brenes, Alejandro J, additional

Published
2022
Full Text
View/download PDF

17. Characterization of Eosinophilic Bronchiectasis: A European Multicohort Study

Author

Shoemark, Amelia, primary, Shteinberg, Michal, additional, De Soyza, Anthony, additional, Haworth, Charles S., additional, Richardson, Hollian, additional, Gao, Yonghua, additional, Perea, Lidia, additional, Dicker, Alison J., additional, Goeminne, Pieter C., additional, Cant, Erin, additional, Polverino, Eva, additional, Altenburg, Josje, additional, Keir, Holly R., additional, Loebinger, Michael R., additional, Blasi, Francesco, additional, Welte, Tobias, additional, Sibila, Oriol, additional, Aliberti, Stefano, additional, and Chalmers, James D., additional

Published
2022
Full Text
View/download PDF

19. Profiling systemic inflammation and neutrophil function in hospitalized patients with COVID19: results from PREDICT-COVID19

Author

Long, Merete, primary, Keir, Holly R, additional, Giam, Yan Hui, additional, Howden, Andrew J M, additional, Brenes, Alejandro J, additional, Rollings, Christina, additional, Pembridge, Thomas, additional, Delgado, Lilia, additional, Abo-Leyah, Hani, additional, Hull, Rebecca C, additional, Gilmour, Amy, additional, Hughes, Chloe, additional, Dowey, Rebecca, additional, Turton, Helena, additional, New, Benjamin J M, additional, Connell, David W, additional, Richardson, Hollian, additional, Thompson, Roger, additional, Condliffe, Alison M, additional, Lamond, Angus I, additional, Cassidy, Diane M, additional, Shoemark, Amelia, additional, Cantrell, Doreen A, additional, and Chalmers, James D, additional

Published
2022
Full Text
View/download PDF

20. Multicentre evaluation of two multiplex PCR platforms for the rapid microbiologicalinvestigation of nosocomial pneumonia in UK ICUs: the INHALE WP1 study

Author

Enne, Virve I, Aydin, Alp, Baldan, Rossella, Owen, Dewi R, Richardson, Hollian, Ricciardi, Federico, Russell, Charlotte, Nomamiukor-Ikeji, Brenda O., Swart, Ann Marie, High, Juliet, Colles, Antony, Barber, Julie A, Gant, Vanya, Livermore, David M, O’Grady, Justin, and Morton, Ben

Subjects
qy_4, qw_4, wc_202, wf_20
Abstract

Background \ud Culture-based microbiological investigation of hospital-acquired or ventilator-associated pneumonia (HAP or VAP) is insensitive, with aetiological agents often unidentified. This can lead to excess antimicrobial treatment of patients with susceptible pathogens, while those with resistant bacteria are treated inadequately for prolonged periods. Using PCR to seek pathogens and their resistance genes directly from clinical samples may improve therapy and stewardship.\ud \ud Methods \ud Surplus routine lower respiratory tract samples were collected from intensive care unit patients about to receive new or changed antibiotics for hospital-onset lower respiratory tract infections at 15 UK hospitals. Testing was performed using the BioFire FilmArray Pneumonia Panel (bioMérieux) and Unyvero Pneumonia Panel (Curetis). Concordance analysis compared machine and routine microbiology results, while Bayesian latent class (BLC) analysis estimated the sensitivity and specificity of each test, incorporating information from both PCR panels and routine microbiology.\ud \ud Findings \ud In 652 eligible samples; PCR identified pathogens in considerably more samples compared with routine microbiology: 60.4% and 74.2% for Unyvero and FilmArray respectively vs 44.2% by routine microbiology. PCR tests also detected more pathogens per sample than routine microbiology. For common HAP/VAP pathogens, FilmArray had sensitivity of 91.7%–100.0% and specificity of 87.5%–99.5%; Unyvero had sensitivity of 50.0%–100.0%%, and specificity of 89.4%–99.0%. BLC analysis indicated that, compared with PCR, routine microbiology had low sensitivity, ranging from 27.0% to 69.4%.\ud \ud Interpretation \ud Conventional and BLC analysis demonstrated that both platforms performed similarly and were considerably more sensitive than routine microbiology, detecting potential pathogens in patient samples reported as culture negative. The increased sensitivity of detection realised by PCR offers potential for improved antimicrobial prescribing.

Published
2022

21. Multicentre evaluation of two multiplex PCR platforms for the rapid microbiological investigation of nosocomial pneumonia in UK ICUs: the INHALE WP1 study

Author

Enne, Virve I, primary, Aydin, Alp, additional, Baldan, Rossella, additional, Owen, Dewi R, additional, Richardson, Hollian, additional, Ricciardi, Federico, additional, Russell, Charlotte, additional, Nomamiukor-Ikeji, Brenda O, additional, Swart, Ann-Marie, additional, High, Juliet, additional, Colles, Antony, additional, Barber, Julie, additional, Gant, Vanya, additional, Livermore, David M, additional, and O’Grady, Justin, additional

Published
2022
Full Text
View/download PDF

22. Rapid diagnosis of bacterial co-infection and antimicrobial resistance in patients with SARS-Cov-2 infection

Author

Richardson, Hollian, primary, Maccallum, Jane, additional, Falconer, Kerry, additional, Cassidy, Diane, additional, Long, Merete, additional, Keir, Holly R, additional, Giam, Yan Hui, additional, Delgado, Lilia, additional, Pembridge, Thomas, additional, Parcell, Benjamin, additional, Haynes, Steven, additional, Abo-Leyah, Hani, additional, Connell, David, additional, and Chalmers, James D, additional

Published
2021
Full Text
View/download PDF

24. Mapping inflammatory endotypes of bronchiectasis associated with impaired mucociliary clearance

Author

Bottier, Mathieu, primary, Cant, Erin, additional, Delgado, Lilia, additional, Cassidy, Diane M, additional, Giam, Yan Hui, additional, Smith, Alexandria, additional, Richardson, Hollian, additional, Dicker, Alison J, additional, Huang, Jeffrey T J, additional, Keir, Holly R, additional, Perea, Lidia, additional, Finch, Simon, additional, Aliberti, Stefano, additional, Sibila, Oriol, additional, Chalmers, James D, additional, and Shoemark, Amelia, additional

Published
2021
Full Text
View/download PDF

25. Characterisation of eosinophilic bronchiectasis: A European multicohort study

Author

Shoemark, Amelia, primary, Shteinberg, Michal, additional, De Soyza, Anthony, additional, Haworth, Charles, additional, Richardson, Hollian, additional, Perea, Lidia, additional, Dicker, Alison, additional, Goeminne, Pieter C, additional, Cant, Erin, additional, Polverino, Eva, additional, Altenburg, Josje, additional, Keir, Holly R, additional, Loebinger, Michael R, additional, Blasi, Francesco, additional, Welte, Tobias, additional, Sibila, Oriol, additional, Aliberti, Stefano, additional, and Chalmers, James D, additional

Published
2021
Full Text
View/download PDF

28. Erratum:Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function (GigaScience DOI: 10.1093/gigascience/giaa053)

Author

Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, Brown, Bonnie L., Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, and Brown, Bonnie L.

Abstract

In the original publication of this article, the author Jiannis Ragoussis was not part of the author byline. His name, affiliation, and funding information have been added to this article. The authors regret this error.

Published
2020

29. Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function

Author

Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, Brown, Bonnie L., Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, and Brown, Bonnie L.

Abstract

Background: Riverine ecosystems are biogeochemical powerhouses driven largely by microbial communities that inhabit water columns and sediments. Because rivers are used extensively for anthropogenic purposes (drinking water, recreation, agriculture, and industry), it is essential to understand how these activities affect the composition of river microbial consortia. Recent studies have shown that river metagenomes vary considerably, suggesting that microbial community data should be included in broad-scale river ecosystem models. But such ecogenomic studies have not been applied on a broad "aquascape"scale, and few if any have applied the newest nanopore technology. Results: We investigated the metagenomes of 11 rivers across 3 continents using MinION nanopore sequencing, a portable platform that could be useful for future global river monitoring. Up to 10 Gb of data per run were generated with average read lengths of 3.4 kb. Diversity and diagnosis of river function potential was accomplished with 0.5-1.0 ⋅ 106 long reads. Our observations for 7 of the 11 rivers conformed to other river-omic findings, and we exposed previously unrecognized microbial biodiversity in the other 4 rivers. Conclusions: Deeper understanding that emerged is that river microbial consortia and the ecological functions they fulfil did not align with geographic location but instead implicated ecological responses of microbes to urban and other anthropogenic effects, and that changes in taxa manifested over a very short geographic space.

Published
2020

30. Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function:[incl. Erratum]

Author

Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne-Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, Brown, Bonnie L., Reddington, Kate, Eccles, David, O'Grady, Justin, Drown, Devin M., Hansen, Lars Hestbjerg, Nielsen, Tue Kjærgaard, Ducluzeau, Anne-Lise, Leggett, Richard M., Heavens, Darren, Peel, Ned, Snutch, Terrance P., Bayega, Anthony, Oikonomopoulos, Spyridon, Ragoussis, Jiannis, Barry, Thomas, van der Helm, Eric, Jolic, Dino, Richardson, Hollian, Jansen, Hans, Tyson, John R., Jain, Miten, and Brown, Bonnie L.

Abstract

Background: Riverine ecosystems are biogeochemical powerhouses driven largely by microbial communities that inhabit water columns and sediments. Because rivers are used extensively for anthropogenic purposes (drinking water, recreation, agriculture, and industry), it is essential to understand how these activities affect the composition of river microbial consortia. Recent studies have shown that river metagenomes vary considerably, suggesting that microbial community data should be included in broad-scale river ecosystem models. But such ecogenomic studies have not been applied on a broad "aquascape" scale, and few if any have applied the newest nanopore technology. Results: We investigated the metagenomes of 11 rivers across 3 continents using MinION nanopore sequencing, a portable platform that could be useful for future global river monitoring. Up to 10 Gb of data per run were generated with average read lengths of 3.4 kb. Diversity and diagnosis of river function potential was accomplished with 0.5-1.0 . 10(6) long reads. Our observations for 7 of the 11 rivers conformed to other river-omic findings, and we exposed previously unrecognized microbial biodiversity in the other 4 rivers. Conclusions: Deeper understanding that emerged is that river microbial consortia and the ecological functions they fulfil did not align with geographic location but instead implicated ecological responses of microbes to urban and other anthropogenic effects, and that changes in taxa manifested over a very short geographic space.

Published
2020

34. Microbiome Use to Stratify Inhaled Corticosteroids in Chronic Obstructive Pulmonary Disease (MUSIC) trial: baseline data and impact of inhaled corticosteroid withdrawal in severe COPD with frequent exacerbations

Author

Richardson, Hollian, primary, Clarke, Clare, additional, Lonergan, Mike, additional, Barclay, Heather, additional, Crichton, Megan, additional, Pollock, Jennifer, additional, Finch, Simon, additional, Billingham, Pauline, additional, Hussain, Furrah, additional, Mackenzie, Andrew, additional, Cassidy, Diane, additional, Band, Margaret, additional, Smith, Andrew, additional, Paracha, Mohammad, additional, Chowdhury, Gourab, additional, Dhasmana, Devesh, additional, Chaudhuri, Rekha, additional, Short, Philip M, additional, Keir, Holly R, additional, Dicker, Alison J, additional, and Chalmers, James D., additional

Published
2020
Full Text
View/download PDF

35. Corrigendum to: Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function

Author

Reddington, Kate, primary, Eccles, David, primary, O'Grady, Justin, primary, Drown, Devin M, primary, Hansen, Lars Hestbjerg, primary, Nielsen, Tue Kjærgaard, primary, Ducluzeau, Anne-Lise, primary, Leggett, Richard M, primary, Heavens, Darren, primary, Peel, Ned, primary, Snutch, Terrance P, primary, Bayega, Anthony, primary, Oikonomopoulos, Spyridon, primary, Ragoussis, Jiannis, primary, Barry, Thomas, primary, van der Helm, Eric, primary, Jolic, Dino, primary, Richardson, Hollian, primary, Jansen, Hans, primary, Tyson, John R, primary, Jain, Miten, primary, and Brown, Bonnie L, primary

Published
2020
Full Text
View/download PDF

36. Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals insight into river microbe taxonomy and function

Author

Reddington, Kate, primary, Eccles, David, primary, O'Grady, Justin, primary, Drown, Devin M, primary, Hansen, Lars Hestbjerg, primary, Nielsen, Tue Kjærgaard, primary, Ducluzeau, Anne-Lise, primary, Leggett, Richard M, primary, Heavens, Darren, primary, Peel, Ned, primary, Snutch, Terrance P, primary, Bayega, Anthony, primary, Oikonomopoulos, Spyridon, primary, Ragoussis, Jiannis, primary, Barry, Thomas, primary, van der Helm, Eric, primary, Jolic, Dino, primary, Richardson, Hollian, primary, Jansen, Hans, primary, Tyson, John R, primary, Jain, Miten, primary, and Brown, Bonnie L, primary

Published
2020
Full Text
View/download PDF

37. Leprosy at the edge of Europe—Biomolecular, isotopic and osteoarchaeological findings from medieval Ireland

Author

Taylor, G. Michael, Murphy, Eileen M., Mendum, Tom A., Pike, Alistair W. G., Linscott, Bethan, Wu, Huihai, O’Grady, Justin, Richardson, Hollian, O’Donovan, Edmond, Troy, Carmelita, and Stewart, Graham R.

Subjects
Bacterial Diseases, Male, Burial, Genotyping Techniques, Social Sciences, Artificial Gene Amplification and Extension, Oxygen Isotopes, Polymerase Chain Reaction, Biochemistry, Osteology, Medicine and Health Sciences, Phylogeny, Middle Aged, Mycobacterium Leprae, Radioactive Carbon Dating, Mitochondrial DNA, Body Remains, Actinobacteria, Nucleic acids, Chemistry, Infectious Diseases, Archaeology, Physical Sciences, Medicine, Female, Anatomy, Research Article, Neglected Tropical Diseases, Chemical Elements, Adult, DNA, Bacterial, Forms of DNA, Science, Research and Analysis Methods, Bone and Bones, Molecular Genetics, Strontium Isotopes, Young Adult, SDG 3 - Good Health and Well-being, Leprosy, Genetics, Humans, Molecular Biology Techniques, Molecular Biology, Chemical Characterization, Isotope Analysis, Bacteria, Organisms, Biology and Life Sciences, DNA, Tropical Diseases, History, Medieval, Strontium, Archaeological Dating, Ireland
Abstract

Relatively little is known of leprosy in Medieval Ireland; as an island located at the far west of Europe it has the potential to provide interesting insights in relation to the historical epidemiology of the disease. To this end the study focuses on five cases of probable leprosy identified in human skeletal remains excavated from inhumation burials. Three of the individuals derived from the cemetery of St Michael Le Pole, Golden Lane, Dublin, while single examples were also identified from Ardreigh, Co. Kildare, and St Patrick’s Church, Armoy, Co. Antrim. The individuals were radiocarbon dated and examined biomolecularly for evidence of either of the causative pathogens, M. leprae or M. lepromatosis. Oxygen and strontium isotopes were measured in tooth enamel and rib samples to determine where the individuals had spent their formative years and to ascertain if they had undertaken any recent migrations. We detected M. leprae DNA in the three Golden Lane cases but not in the probable cases from either Ardreigh Co. Kildare or Armoy, Co. Antrim. M. lepromatosis was not detected in any of the burals. DNA preservation was sufficiently robust to allow genotyping of M. leprae strains in two of the Golden Lane burials, SkCXCV (12-13th century) and SkCCXXX (11-13th century). These strains were found to belong on different lineages of the M. leprae phylogenetic tree, namely branches 3 and 2 respectively. Whole genome sequencing was also attempted on these two isolates with a view to gaining further information but poor genome coverage precluded phylogenetic analysis. Data from the biomolecular study was combined with osteological, isotopic and radiocarbon dating to provide a comprehensive and multidisciplinary study of the Irish cases. Strontium and oxygen isotopic analysis indicate that two of the individuals from Golden Lane (SkCXLVIII (10-11th century) and SkCXCV) were of Scandinavian origin, while SkCCXXX may have spent his childhood in the north of Ireland or central Britain. We propose that the Vikings were responsible for introducing leprosy to Ireland. This work adds to our knowledge of the likely origins of leprosy in Medieval Ireland and will hopefully stimulate further research into the history and spread of this ancient disease across the world.

Published
2018

38. Presence of Mycobacterium avium subspecies paratuberculosis monitored over varying temporal and spatial scales in river catchments: persistent routes for human exposure

Author

Richardson, Hollian, Rhodes, Glenn, Henrys, Peter, Sedda, Luigi, Weightman, Andrew J., Pickup, Roger W., Richardson, Hollian, Rhodes, Glenn, Henrys, Peter, Sedda, Luigi, Weightman, Andrew J., and Pickup, Roger W.

Abstract

Mycobacterium avium subspecies paratuberculosis (Map) was monitored by quantitative PCR over a range of temporal and spatial scales in the River Tywi catchment. This study shows the persistence of Map over a 10-year period with little change, which correlates with the recognised levels of Johne’s disease in British herds over that period (aim 1). Map was quantified within the river at up to 108 cell equivalents L−1 and was shown to be consistently present when monitored over finer timescales (aim 4). Small wastewater treatment plants where the ingress of human-associated Map might be expected had no significant effect (aim 2). Map was found for the first time to be located in natural river foams providing another route for spread via aerosols (aim 5). This study provides evidence for the environmental continuum of Map from the grazing infected animal via rain driven runoff through field drains and streams into main rivers; with detection at a high frequency throughout the year. Should Map need to be monitored in the future, we recommend that weekly or monthly sampling from a fixed location on a river will capture an adequate representation of the flow dynamics of Map in a catchment (aim 3). The human exposure to Map during this process and its impact on human health remains unquantified.

Published
2019

39. Presence of Mycobacterium avium Subspecies paratuberculosis Monitored Over Varying Temporal and Spatial Scales in River Catchments:persistent Routes for Human Exposure.

Author

Richardson, Hollian, Rhodes, Glenn, Henrys, P.A, Sedda, Luigi, Weightman, Andrew J., Pickup, Roger, Richardson, Hollian, Rhodes, Glenn, Henrys, P.A, Sedda, Luigi, Weightman, Andrew J., and Pickup, Roger

Abstract

Mycobacterium avium subspecies paratuberculosis (Map) was monitored by quantitative PCR over a range of temporal and spatial scales in the River Tywi catchment. This study shows the persistence of Map over a 10-year period with little change, which correlates with the recognised levels of Johne’s disease in British herds over that period (aim 1). Map was quantified within the river at up to 108 cell equivalents L−1 and was shown to be consistently present when monitored over finer timescales (aim 4). Small wastewater treatment plants where the ingress of human-associated Map might be expected had no significant effect (aim 2). Map was found for the first time to be located in natural river foams providing another route for spread via aerosols (aim 5). This study provides evidence for the environmental continuum of Map from the grazing infected animal via rain driven runoff through field drains and streams into main rivers; with detection at a high frequency throughout the year. Should Map need to be monitored in the future, we recommend that weekly or monthly sampling from a fixed location on a river will capture an adequate representation of the flow dynamics of Map in a catchment (aim 3). The human exposure to Map during this process and its impact on human health remains unquantified.

Published
2019

40. Inflammatory molecular endotypes in bronchiectasis

Author

Shoemark, Amelia, primary, Smith, Alexandria, additional, Giam, Ashley, additional, Dicker, Alison, additional, Richardson, Hollian, additional, Huang, Jeffrey T J, additional, Keir, Holly R, additional, Finch, Simon, additional, Aliberti, Stefano, additional, Sibila, Oriol, additional, and Chalmers, James D, additional

Published
2019
Full Text
View/download PDF

43. Leprosy at the edge of Europe—Biomolecular, isotopic and osteoarchaeological findings from medieval Ireland

Author

Taylor, G. Michael, primary, Murphy, Eileen M., additional, Mendum, Tom A., additional, Pike, Alistair W. G., additional, Linscott, Bethan, additional, Wu, Huihai, additional, O’Grady, Justin, additional, Richardson, Hollian, additional, O’Donovan, Edmond, additional, Troy, Carmelita, additional, and Stewart, Graham R., additional

Published
2018
Full Text
View/download PDF

45. Rapid Diagnosis of Lower Respiratory Infection using Nanopore-based Clinical Metagenomics

Author

Charalampous, Themoula, primary, Richardson, Hollian, additional, Kay, Gemma L., additional, Baldan, Rossella, additional, Jeanes, Christopher, additional, Rae, Duncan, additional, Grundy, Sara, additional, Turner, Daniel J., additional, Wain, John, additional, Leggett, Richard M., additional, Livermore, David M., additional, and O’Grady, Justin, additional

Published
2018
Full Text
View/download PDF

46. ONT Library Preparation v1

Author

Reddington, Kate, primary, Eccles, David, additional, O’Grady, Justin, additional, M., Devin, additional, Hestbjerg, Lars, additional, Kjærgaard, Tue, additional, Ducluzeau, Anne-Lise, additional, M., Richard, additional, Heavens, Darren, additional, Peel, Ned, additional, P., Terrance, additional, Bayega, Anthony, additional, Oikonomopoulos, Spyros, additional, Ragoussis, Jiannis, additional, Barry, Thomas, additional, van, Eric, additional, Jolic, Dino, additional, Richardson, Hollian, additional, Jansen, Hans, additional, R., John, additional, Jain, Miten, additional, and L., Bonnie, additional

Published
2018
Full Text
View/download PDF

47. Collection and purification of river water samples v1

Author

Reddington, Kate, primary, Eccles, David, additional, O’Grady, Justin, additional, M., Devin, additional, Hestbjerg, Lars, additional, Kjærgaard, Tue, additional, Ducluzeau, Anne-Lise, additional, M., Richard, additional, Heavens, Darren, additional, Peel, Ned, additional, P., Terrance, additional, Bayega, Anthony, additional, Oikonomopoulos, Spyros, additional, Ragoussis, Jiannis, additional, Barry, Thomas, additional, van, Eric, additional, Jolic, Dino, additional, Richardson, Hollian, additional, Jansen, Hans, additional, R., John, additional, Jain, Miten, additional, and L., Bonnie, additional

Published
2018
Full Text
View/download PDF

48. DNA extraction and purification for MARC phase 3 global river water sequencing v1

Author

Reddington, Kate, primary, Eccles, David, additional, O’Grady, Justin, additional, M., Devin, additional, Hestbjerg, Lars, additional, Kjærgaard, Tue, additional, Ducluzeau, Anne-Lise, additional, M., Richard, additional, Heavens, Darren, additional, Peel, Ned, additional, P., Terrance, additional, Bayega, Anthony, additional, Oikonomopoulos, Spyros, additional, Ragoussis, Jiannis, additional, Barry, Thomas, additional, van, Eric, additional, Jolic, Dino, additional, Richardson, Hollian, additional, Jansen, Hans, additional, R., John, additional, Jain, Miten, additional, and L., Bonnie, additional

Published
2018
Full Text
View/download PDF

49. Nanopore sequencing and assembly of a human genome with ultra-long reads

Author

Jain, Miten, primary, Koren, Sergey, additional, Miga, Karen H, additional, Quick, Josh, additional, Rand, Arthur C, additional, Sasani, Thomas A, additional, Tyson, John R, additional, Beggs, Andrew D, additional, Dilthey, Alexander T, additional, Fiddes, Ian T, additional, Malla, Sunir, additional, Marriott, Hannah, additional, Nieto, Tom, additional, O'Grady, Justin, additional, Olsen, Hugh E, additional, Pedersen, Brent S, additional, Rhie, Arang, additional, Richardson, Hollian, additional, Quinlan, Aaron R, additional, Snutch, Terrance P, additional, Tee, Louise, additional, Paten, Benedict, additional, Phillippy, Adam M, additional, Simpson, Jared T, additional, Loman, Nicholas J, additional, and Loose, Matthew, additional

Published
2018
Full Text
View/download PDF

50. Mycobacterium avium subspecies paratuberculosis: human exposure through environmental and domestic aerosols

Author

Rhodes, Glenn, Richardson, Hollian, Hermon-Taylor, John, Weightman, Andrew, Higham, Andrew, Pickup, Roger, Rhodes, Glenn, Richardson, Hollian, Hermon-Taylor, John, Weightman, Andrew, Higham, Andrew, and Pickup, Roger

Abstract

Mycobacterium avium subspecies paratuberculosis (Map) causes Johne’s disease in animals and is significantly associated with Crohn’s disease (CD) in humans. Our previous studies have shown Map to be present in UK rivers due to land deposition from chronic livestock infection and runoff driven by rainfall. The epidemiology of CD in Cardiff showed a significant association with the river Taff in which Map can be detected on a regular basis. We have previously hypothesised that aerosols from the river might influence the epidemiology of CD. In this preliminary study, we detected Map by quantitative PCR in one of five aerosol samples collected above the river Taff. In addition, we examined domestic showers from different regions in the UK and detected Map in 3 out of 30 independent samples. In detecting Map in river aerosols and those from domestic showers, this is the first study to provide evidence that aerosols are an exposure route for Map to humans and may play a role in the epidemiology of CD.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results