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7 results on '"Riet, Jade"'

1. A native strain of Bacillus subtilis increases lipid accumulation and modulates expression of genes related to digestion and amino acid metabolism in Litopenaeus vannamei

Author

Medeiros, Luiza, Dall'Agno, Laura, Riet, Jade, Nornberg, Bruna, Azevedo, Raíza, Cardoso, Arthur, da Silva, Jéssica Lucinda Saldanha, de Sousa, Oscarina Viana, Rosas, Victor Torres, Tesser, Marcelo Borges, Pedrosa, Virgínia F., Romano, Luis A., Wasielesky, Wilson, Jr, and Marins, Luis F.

Published
2024
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3. A native strain of Bacillus subtilis increases lipid accumulation and modulates expression of genes related to digestion and amino acid metabolism in Litopenaeus vannamei

Author

Medeiros, Luiza, primary, Dall'Agno, Laura, additional, Riet, Jade, additional, Nornberg, Bruna, additional, Azevedo, Raíza, additional, Cardoso, Arthur, additional, da Silva, Jéssica Lucinda Saldanha, additional, de Sousa, Oscarina Viana, additional, Rosas, Victor Torres, additional, Tesser, Marcelo Borges, additional, Pedrosa, Virgínia F., additional, Romano, Luis A., additional, Wasielesky, Wilson, additional, and Marins, Luis F., additional

Published
2023
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4. Bacillus subtilis expressing double-strand RNAs (dsRNAs) induces RNA interference mechanism (RNAi) and increases survival of WSSV-challenged Litopenaeus vannamei

Author

Riet, Jade, primary, Costa-Filho, João, additional, Dall'Agno, Laura, additional, Medeiros, Luiza, additional, Azevedo, Raíza, additional, Nogueira, Luiz F., additional, Maggioni, Rodrigo, additional, Pedrosa, Virgínia F., additional, Romano, Luis A., additional, Altenbuchner, Josef, additional, Wasielesky, Wilson, additional, and Marins, Luis F., additional

Published
2021
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6. Genetic manipulation of native Bacillus cereus: a biotechnological tool for aquaculture.

Author

Costa Filho, João, Riet, Jade, Santos, Kamila, de Sousa, Oscarina Viana, Maggioni, Rodrigo, Feijó, Rubens Galdino, Wasielesky Jr., Wilson, and Marins, Luis Fernando

Subjects
*WHITELEG shrimp, *BACILLUS cereus, *SHRIMPS, *GROWTH plate, *WATER quality, *FISH genetics
Abstract

This work aimed to perform the genetic manipulation of the native B. cereus #25 strain and to evaluate the colonization in the intestinal tract of Litopenaeus vannamei. The expression of the GFPmut1, absence of amylase activity, and growth in spectinomycin plates confirmed the transformation of the strain (BC25GM). In the shrimp bioassay, its addiction in the feed showed non-difference for water quality, growth performance, and toxic effects for L. vannamei (survival 100%). The presence of cells expressing GFPmut1 in the midgut of the animals was observed, suggesting that the genetically engineered strain colonized the shrimp intestines. The relative fluorescence was 35% higher in the midgut of the shrimps feed with BC25GM. In conclusion, the present work showed that it is possible to manipulate a native B. cereus strain with the nonspecific pSG1154 plasmid. We reported the possibility to implement native strains as a biotechnology tool applied in aquaculture, especially to deliver beneficial molecules for shrimps. [ABSTRACT FROM AUTHOR]

Published
2022
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