107 results on '"Rigidoporus lignosus"'
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2. In vitro and in vivo synthetic fungicides control of Rigidoporus microporus on Para rubber in Nigeria
- Author
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Nicholas O. O. Ogbebor, Adefunke T. Adekunle, Nosakhare O. Eghafona, and Ikponmwosa A. Ogboghodo
- Subjects
hevea brasiliensis ,chemical control ,rigidoporus lignosus ,Agriculture - Abstract
Rigidoporus microporus is a major threat to Hevea brasiliensis in the rubber growing regions of the world. In vitro synthetic fungicidesensitivity assays of Tridemorph, Benomyl and Bayfidan at seven different concentrations, and in vivo effectiveness of the three fungicides on R. microporus inoculated on rubber seedlings were evaluated. The three chemical fungicides used in this study were effective on R. microporus, with Tridemorph being the most effective. The minimum inhibitory concentration value for Bayfidan was between fungicide concentrations of 0.001µg/ml and 0.005µg/ml. Percentage of plant death and presence of rhizomorph recorded at two months after inoculation were higher than that recorded at the termination of five months experimental period after inoculation. Tridemorphtreatment had the highest plant health both at two months and at five months after inoculation. Decline in plant death from the third months onward suggested a reduction in fungal activity of R. microporus. Tridemorph was most effective among the three fungicides tested as it exhibited higher mycelial percentage inhibition in the management of R. microporus in vitro and higher plant health of rubber seedlings in vivo. more...
- Published
- 2015
Catalog
3. In vitro Evaluation of the Fungicidal Potential of Aqueous and Methanolic Extracts of Thevetia peruviana on the Development of Rigidoporus lignosus, Causal Agent of White Root rot of Hevea brasiliensis
- Author
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Alain Heu, Jules Patrice Ngoh Dooh, Martial Douanla Ajebe, Zachée Ambang, Godswill Ntsomboh Ntsefong, Abdou Nourou Kone Nsangou, and Serge Bertrand Mboussi
- Subjects
Fungicide ,Horticulture ,Thevetia ,biology ,Root rot ,Hevea brasiliensis ,Rigidoporus lignosus ,biology.organism_classification ,Microbiology ,In vitro - Abstract
Aims: The objective of this work is to evaluate the effect of (aqueous (EAq) and methanolic (ME)) extracts of Thevetia peruviana on the in vitro development of Rigidoporus lignosus. Study Design: A synthetic fungicide (Onazol 100), two extracts at differents concentrations: C1 = 3.5 mg / ml; C2 = 7 mg / ml; C3 = 15 mg / ml; C4 = 30 mg / ml; C5 = 50 mg / ml and C6 = 100 mg / ml for EAq; C1 = 3.5 ml/ml; C2 = 7 ml/ml; C3 = 15 ml/ml; C4 = 30 ml/ml; C5 = 50 ml/ml for ME and a negative control (T = 0 mg / ml) were used. Place and Duration of Study: The study was conducted in pathology laboratory at University of Yaounde I and IRAD of Nkolbisson in 2018. Methodology: Daily measurements of the pathogen's development was used to evaluate the average growth (D) of the mycelium. The inhibition percentages of the different doses of EAq, ME and ONAZOL 100 were calculated after 4 to 5 days of growth of the fungus. Results: The major result obtained compared to the rate of rot showed that the treatments EAq, ME (C6) and ONAZOL 100 were closed. The disease rates at different concentrations of C1 to C6 were 13.91 to 100% for ME, 9.34 to 100% for EAq, and 100% for ONAZOL 100. Conclusion: The two extracts are promising, and on small and medium scale, could be an effective and cheap formulation for the control of Rigidoporus lignosus. more...
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- 2021
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4. UJI AKTIVITAS ANTIBIOSIS PSEUDOMONADS PENDARFLUOR TERHADAP Rigidoporus lignosus (Klotszch) Imazeki PENYEBAB PENYAKIT AKAR PUTIH
- Author
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Hasanuddin
- Subjects
Biological control ,Rigidoporus lignosus ,Pseudomonas fluorescens ,P. aeruginosa ,antibiosis activity ,Plant culture ,SB1-1110 - Abstract
The potential of fluorescent bacteria as biological control agents for white root disease caused by Rigidoporus lignosus has been investigated. Isolation of bacteria from the soil using S1 media produced two fluorescent bacteria isolates. Using the Microbact 12A+12B method, both bacteria were identified as Pseudomonas fluorescens and P. aeruginosa. These two species of bacteria were then used as antibiosis activity test against R. lignosus. Four series antibiosis activity tests were done, that were antibiosis test of media culture bacteria growth to R. lignosus colony, antibiosis test of dry fluorescent pigment extract to R. lignosus, influence of Fe3+ to antibiosis activity of bacteria test, and affinity of media supernatant to Fe3+. The results were: antibiosis activity of King’s B (KB) media was more effective than media 523 in the inhibition of R. lignosus colony growth. There was no significant different antibiosis activity of dry fluorescent pigment extract from media KB and media 523 in the inhibition of R. lignosus colony growth. The level of Fe3+ in the media might influence antibiosis activity of fluorescent pigment. Affinity test of KB supernatant from fluorescent bacteria culture with Fe3+ showed an absorption peak of 410 nm on spectrophotometer, and none for the fungi. These results indicate that P. fluorescens and P. aeruginosa produce cathecol-type siderophore with high affinity against Fe3+ compared with hydroxamate-type siderophore which is generally produced by fungus. more...
- Published
- 2011
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5. EFEKTIVITAS BIOFUNGISIDA Trichoderma koningii TERHADAP PENCEGAHAN PENYAKIT JAMUR AKAR PUTIH DI PEMBIBITAN BATANG BAWAH TANAMAN KARET (Hevea brasiliensis)
- Author
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Gema Tarigan, Guntoro, and Mardiana Wahyuni
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Horticulture ,Trichoderma koningii ,biology ,Seedling ,technology, industry, and agriculture ,Randomized block design ,Fungus ,Rigidoporus lignosus ,biology.organism_classification - Abstract
One of the main obstacles in rubber cultivation is the white root fungus (JAP) attack which caused by Rigidophorus ligonosus. This pathogen infects rubber plants from the nursery until the mature plants. Efforts to control the disease have been carried out by chemical, technical culture and use of biological agencies. This research was conducted at the STIP-AP Medan in January 2017 to June 2017, this study used a non-factorial Randomized Block Design (RBD) with four replications and four treatments. Using Trichoderma koningii with somes doses were 20 grams, 30 grams, and 40 grams/seedling. The results of this study indicate that the biofungicide application of Trichoderma koningii 30 g/seedling produces the greatest inhibition of the rubber plants seedling nursery against the growth of pathogens Rigidoporus lignosus. more...
- Published
- 2019
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6. Biological control of Rigidoporus lignosus in Hevea brasiliensis in Nigeria.
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Ogbebor, Nicholas Obehi, Adekunle, Adefunke Temitayo, Eghafona, Odeh Nosakhare, and Ogboghodo, Abraham Ikponmwosa
- Subjects
- *
PHYSIOLOGICAL control systems , *RIGIDOPORUS lignosus , *HEVEA , *ANTAGONISTIC fungi , *IN vitro studies - Abstract
The study was conducted to evaluate in vitro and in vivo control of fungal antagonists on Rigidoporus lignosus (klotzsch) Imaz in Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. in the rain forest zone of Nigeria. In vitro assessments of biological agents were carried out in dixenic cultures in Petri plates. In vivo tests were carried out in the nursery using Hypocrea virens and Hypocrea jecorina . Significant differences were observed with the dual inoculations of fungal antagonists and R. lignosus inoculated the same day, and the antagonists inoculated 24 h before inoculation of R. lignosus . Hypocrea jecorina was most effective in the control of R. lignosus with percentage inhibition of 86.83 %. Hypocrea virens , H. jecorina , Trichoderma spirale , Trichoderma sp., Trichoderma sp. Pers, and Hypocrea lixii were effective on R. lignosus . Fungal antagonists inoculated 24 h before inoculation of R. lignosus exhibited higher inhibitory efficacies than antagonists and R. lignosus inoculated the same day. In the in vivo evaluation of fungi antagonists on R. lignosus , seedlings in H. jecorina treatment at 60 d and H. virens treatment at 150 d after inoculations respectively had the highest plant heaths (highest length of stem and length of tap rot, lowest plant death, and least foliar symptom) compared to the control. Mortality rate was higher at 60 d after inoculation and decline from the third months onward. Significant differences were observed between the control and R. lignosus treatment in all the parameters evaluated. [ABSTRACT FROM AUTHOR] more...
- Published
- 2015
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7. Kinetic role of a histidine residue in the T1 copper site of the laccase from Rigidoporus lignosus.
- Author
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Vianello, Fabio, Miotto, Giovanni, Cambria, Maria Teresa, Lima, Giuseppina P.P., Vanzani, Paola, and Di Paolo, Maria Luisa
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RIGIDOPORUS lignosus , *HISTIDINE , *COPPER , *LACCASE , *FUNGAL enzymes , *DERIVATIZATION , *CATALYTIC activity , *PH effect - Abstract
Highlights: [•] The RIL activity at low pH value is controlled by an ionizable residue present in its active site. [•] DEPC derivatization of accessible His of RlL affect the kinetic behavior of RlL with pH and ionic strength. [•] Main role of His-457 in the control of the catalytic activity of laccase from Rigidoporus lignosus. [Copyright &y& Elsevier] more...
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- 2014
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8. Enhanced Laccase Production in White-Rot Fungus Rigidoporus lignosus by the Addition of Selected Phenolic and Aromatic Compounds.
- Author
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Cambria, Maria Teresa, Ragusa, Santa, Calabrese, Vittorio, and Cambria, Antonio
- Abstract
The white rot fungus Rigidoporus lignosus produces substantial amounts of extracellular laccase, a multicopper blue oxidase which is capable of oxidizing a wide range of organic substrates. Laccase production can be greatly enhanced in liquid cultures supplemented with various aromatic and phenolic compounds. The maximum enzyme activity was reached at the 21st or 24th day of fungal cultivation after the addition of inducers. The zymograms of extracellular fluid of culture preparation in the presence of inducers, at maximum activity day, revealed two bands with enzymatic activity, called Lac1 and Lac2, having different intensities. Lac2 band shows the higher intensity which changed with the different inducers. Laccase induction can be also obtained by adding to the culture medium olive mill wastewaters, which shows a high content of phenolic compounds [ABSTRACT FROM AUTHOR] more...
- Published
- 2011
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9. Efficacy of application time of Penicillium sp. suspension on White Root Fungus (Rigidoporus lignosus) in Nutmeg (Myristica fragrans)
- Author
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Agustinur, Chairudin, and J. Permadi
- Subjects
Horticulture ,biology ,Penicillium ,food and beverages ,Myristica fragrans ,Nutmeg ,Fungus ,Rigidoporus lignosus ,biology.organism_classification ,Suspension (vehicle) ,Application time - Abstract
Cultivation of nutmeg (Myristica fragrans) is running into several problems, one of these is caused by white root fungus (Rigidoporus lignosus) which causes losses to nutmeg farmers. This fungus is known to be controlled by biological control using microorganisms such as Penicillium sp. This research was conducted to determine the efficacy of application time of Penicillium sp. crude extract against white root fungus (Rigidoporus lignosus) in nutmeg plants. The design used in this study was a non-factorial completely randomized design with the main factor being the application time of Penicilllium sp. crude extract with 4 treatments. Each treatment on 7 replicates. Control without suspension application Penicillium sp. (PK0), Application of Penicillium sp. 7 days before inoculation of Rigidoporus lignosus (PK1), Application of suspension of Penicillium sp. concurrently with Rigidoporus lignosus (PK2) inoculation, Application of suspension of Penicillium sp. 7 days after Rigidoporus lignosus (PK3) inoculation. Total are 28 experimental units. In this case, the test plants used are 11 months old nutmeg. The research parameters consisted of the incubation period, disease incidence and attack intensity. The results showed that the treatment at one-time application of the suspension of Penicillium sp. Significantly affect the intensity of attacks and the incidence of infectious disease pathogens Rigidoporus lignosus. Application time for Penicillium sp. 7 days before the suspension of Rigidoporus lignosus is the best offer in the attack of Rigidoporus lignosus disease. more...
- Published
- 2021
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10. Distribution of Rigidoporus lignosus genotypes in a rubber plantation, as revealed by somatic compatibility.
- Author
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Suwandi Harman Hamidson and Shigeo Naito
- Subjects
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SPATIAL ecology , *SOMATIC cells , *RIGIDOPORUS lignosus , *WHITE root disease of hevea , *PLANT diseases - Abstract
A study was conducted to determine the spatial distribution of somatic incompatibility groups (SIGs) in Rigidoporus lignosus populations responsible for white root disease in a south Sumatera rubber plantation. Most isolates colonizing the stumps were somatically compatible with isolates from either a nearby stump or a living rubber tree, which suggested evidence of stump-to-tree or stump-to-stump clonal growth of the fungus. SIGs of R. lignosus occupied an area territorially in which the minimum size estimate for the largest genotype was 25?m in length. [ABSTRACT FROM AUTHOR] more...
- Published
- 2004
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11. Structure–activity relationship on fungal laccase from Rigidoporus lignosus: a Fourier-transform infrared spectroscopic study.
- Author
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Ragusa, Santa, Cambria, Maria Teresa, Pierfederici, Francesco, Scirè, Andrea, Bertoli, Enrico, Tanfani, Fabio, and Cambria, Antonio
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LACCASE , *RIGIDOPORUS lignosus , *ENZYMES , *FOURIER transform infrared spectroscopy - Abstract
The structure and thermal stability of a laccase from Rigidoporus lignosus (Rl) was analysed by Fourier-transform infrared (FT-IR) spectroscopy. The enzyme was depleted of copper atoms, then part of the apoenzyme was re-metalled and these two forms of the protein were analysed as well. The enzymatic activity, lost by the removal of copper atoms, was restored in the re-metalled apoenzyme and resulted similar to that of native protein. The infrared data indicated that the enzyme contains a large amount of β-sheets and a small content of α-helices, and it displayed a marked thermostability showing the Tm at 92.5 °C. The apoenzyme and the re-metalled apoenzyme did not show remarkable differences in the secondary structure with respect to the native protein, but the thermal stability of the apoenzyme was dramatically reduced showing a Tm close to 72 °C, while the re-metalled protein displayed the Tm at 90 °C. These data indicate that copper atoms, beside their role in catalytic activity, play also an important role on the stabilisation of the structure of Rl laccase. About 35% of the polypeptide chain is buried and/or constitutes a particular compact structure, which, beside copper atoms, is probably involved in the high thermal stability of the protein. Another small part of the structure is particularly sensitive to high temperatures and it could be the cause of the loss of enzymatic activity when the temperature is raised above 45–50 °C. [Copyright &y& Elsevier] more...
- Published
- 2002
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12. Comparative study for detecting Rigidoporus lignosus on rubber trees.
- Author
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Guyot, Jean and Flori, Albert
- Subjects
RIGIDOPORUS lignosus ,WHITE root disease of hevea - Abstract
Early detection of root diseases is a great problem in perennial crops, especially in rubber tree. Planters are continually confronted with the balance of efficiency/cost of the detection. Therefore, a trial was set up to compare three sampling densities and four exposure techniques for detection of white root rot in rubber plantings. A complete census of all trees is the only efficient method of detection in a control program. When reducing the sampling density, a lot of infected trees are not detected and they remain an inoculum source for the spread of the disease. Mulching for one month followed by root exposure is the best technique to reveal the presence of the fungus. The sampling density is of greater significance than the infection exposure method. [Copyright &y& Elsevier] more...
- Published
- 2002
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13. Structural and Kinetic Characterization of Native Laccases from Pleurotus ostreatus, Rigidoporus lignosus, and Trametes trogii.
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Garzillo, Anna, Colao, Maria, Buonocore, Vincenzo, Oliva, Romina, Falcigno, Lucia, Saviano, Michele, Santoro, Anna, Zappala, Riccardo, Bonomo, Raffaele, Bianco, Carmelina, Giardina, Paola, Palmieri, Gianna, and Sannia, Giovanni more...
- Abstract
A comparative study has been performed on five native laccases purified from the three basidiomycete fungi Pleurotus ostreatus, Rigidoporus lignosus, and Trametes trogii to relate their different catalytic capacities to their structural properties. Spectroscopic absorption features and EPR spectra at various pH values of the five enzymes are very similar and typical of the blue oxidases. The analysis of the dependence of kinetic parameters on pH suggested that a histidine residue is involved in the binding of nonphenolic substrates, whereas both a histidine and an acidic residue may be involved in the binding of phenolic compounds. His and an Asp residue are indeed found at the bottom of a cavity which may be regarded as a suitable substrate channel for approaching to type 1 copper in the 3D homology models of the two laccases from Pleuorotus ostreatus (POXC and POXA1b) whose sequences are known. [ABSTRACT FROM AUTHOR] more...
- Published
- 2001
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14. Ultrastructure of Laticifers Modifications in Hevea brasiliensis Infected with Root Rot Fungi.
- Author
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Nicole, M., Geiger, J. P., and Nandris, D.
- Subjects
- *
FUNGI , *PLANT diseases , *COAGULATION , *RIGIDOPORUS lignosus , *PHELLINUS noxius , *LYSOSOMES - Abstract
This paper presents some aspects of latex coagulation inside latice vessels of roots of Hevea brasiliensis infected by two fungi: Rigidoporus lignosus and Phellinus noxius. Three stages are described in latex coagulation: the phase of latex destabilisation characterized by the bursting of vacuoles and lysosomes membranes; the phase of latex coagulation characterized by the fusion of rubber particles and the disorganization of the cytoplasm; the formation of shots of rubber clumps indicating the final stage of coagulation. [ABSTRACT FROM AUTHOR] more...
- Published
- 1986
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15. Modelling rubber-tree root diseases, simulations of various inoculum rates and methods of control.
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Nandris, D., Chadoeuf, J., Pierrat, J. C., Joannes, H., Geiger, J. P., and Nicole, M.
- Subjects
- *
PLANT diseases , *RUBBER plants , *HEVEA , *RIGIDOPORUS lignosus , *PHELLINUS noxius - Abstract
Two root-rot fungi, Rigidoporus lignosus and Phellinus noxius are the causal agents of severe damage in Ivory Coast rubber tree, Hevea brasiliensis, plantations. Mathematical investigations were made using data from a previous 6 year long epidemiological survey. Modelling of spatial spread of the pathogens and mortality of the infected trees, lead to simulations of the quantitative evolution versus time of root diseases. The theoretical kinetics obtained revealed intra and interspecific variability among the studied stands with respect to the damage caused. more...
- Published
- 1996
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16. Variation in virulence among Rigidoporus lignosus and Phellinus noxius isolates from West Africa.
- Author
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Nandris, D., Nicole, M., and Geiger, J. P.
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HEVEA , *RUBBER plants , *MICROBIAL virulence , *SOIL moisture , *RIGIDOPORUS lignosus , *PHELLINUS noxius - Abstract
Pathogenicity of isolates of R. lignosus and P. noxius collected from rubber and forest trees, was tested on young rubber plants (Hevea brasiliensis) using a technique of artificial inoculation under controlled conditions (e. g. soil moisture). After two months, almost all the plants inoculated with various isolated of R. lignosus were infected and differences in virulence were apparent. After five months, these differences were reflected in the mortality rates of the inoculated plants. For some isolates the attack begins early and abruptly, whereas for others, symptoms appear more gradually. With P. noxius, differences appear also very distinctly in the pathogenicity among the isolates during the entire duration of the experiment. Principal-component analysis of the data recorded has demonstrated variations in pathogenicity among populations of R. lignosus and of P. noxius. The fact must be borne in mind when control methods of these diseases of rubber tree are developed. [ABSTRACT FROM AUTHOR] more...
- Published
- 1987
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17. Ultrastructural aspects of rubber tree root rot diseases.
- Author
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Nicole, M., Geiger, J. P., and Nandris, D.
- Subjects
- *
HEVEA , *PLANT roots , *RIGIDOPORUS lignosus , *PHELLINUS noxius , *HYPHAE of fungi , *RUBBER plants - Abstract
The aggression of Hevea brasiliensis roots by two root-rot fungi, Rigidoporus lignosus and Phellinus noxius is studied at cellular level. Ultrastructural observations reveal the root penetration and tissues colonization by the fungal hyphae, showing clearly alteration in the host cell wall, leading to the root decays This paper describes the different mechanisms used by these two fungi in degrading lignified and non lignified tissues. [ABSTRACT FROM AUTHOR] more...
- Published
- 1987
- Full Text
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18. Biodegradation of Hevea brasiliensis wood by Rigidoporus lignosus and Phellinus noxius.
- Author
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Geiger, J.-P., Rio, B., Nicole, M., and Nandris, D.
- Subjects
- *
BIODEGRADATION , *HEVEA , *RIGIDOPORUS lignosus , *PHELLINUS noxius , *LIGNINS , *WOOD chemistry - Abstract
The study of the degradation of slats shows that P. noxius, just as R. lignosus, is capable of degrading lignin. Both pathogens are thus white rot fungi. In this framework, the comparison of the behaviour of these two fungi with that of Antropodia sp. , a known brown rot agent, is particularly illustrative. Lignin assays showed the tendency of R. lignosus to preferentially degrade lignin, while P. noxius preferentially degrades the polysaccharide fraction. Finally, both fungi also cause considerable changes in the residual lignin by modifying the monomer composition of the polymer. In vivo and in comparison to healthy tissues, there is a significant increase in the lignin content of infested tissues removed from the front of R. lignosus progression. It would appear that Hevea reacts by an increased synthesis of lignin. This reaction is ineffective, since the pathogen ultimately degrades the polymer. Such reaction is much less evident in case of attack by P. noxius. In terms of the monomer composition of lignin, there are changes in the tissues colonized for the longest times, comparable to that observed in vitro. [ABSTRACT FROM AUTHOR] more...
- Published
- 1986
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19. The aggression of Hevea brasileinsis by Rigidoporus lignosus and Phellinus noxius: some biochemical events.
- Author
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Nandris, D., Geiger, J. P., Rio, B., and Nicole, M.
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- *
PARASITES , *PHELLINUS noxius , *RIGIDOPORUS lignosus - Published
- 1985
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20. Variability among African populations of Rigidoporus lignosus and Phellinus noxius.
- Author
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Nandris, D., Geiger, J. P., Rio, B., and Nicole, M.
- Subjects
- *
FUNGI , *PHELLINUS noxius , *RIGIDOPORUS lignosus - Published
- 1985
- Full Text
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21. New method of direct control of Rigidoporus lignosus, causing agent of white root rot of Hevea.
- Author
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Canh, Tran van
- Subjects
- *
THERAPEUTICS , *FUNGI , *RIGIDOPORUS lignosus - Published
- 1985
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22. Artificial infections of young plants of Hevea brasiliensis by Rigidoporous lignosus (Kl.) Imaz. and Phellinus noxius (Corner) G. H. Cunn.
- Author
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Nicole, M., Geiger, J. P., and Nandris, D.
- Subjects
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FUNGI , *PARASITIC plants , *PLANT diseases , *RIGIDOPORUS lignosus , *RIGIDOPORUS , *PHELLINUS noxius , *HYMENOCHAETACEAE - Abstract
In vitro and in vivo inoculation methods with two rooting fungi were tested on young rubber trees. In vitro, experimentation did not succeed. In vivo, under greenhouse conditions, the effect of different parameters has been quantified and a practical method for infecting plants is proposed. With Rigidoporus lignosus, an artificial inoculum i.e. eleven months preinfected wood segments of rubber branches, produced the best infections. Because of the particular biology of this fungus (pathogenicity controlled by an aerobic micro-environment), saturation of the soil of the inoculation pots increased plant mortality significantly. With Phellinus noxius, a similar method of inoculation has to be used. However, study of the effect of age of inoculum on the severity of attack revealed a better efficacy of a five months old inoculum. The nonexistence of rhizomorphs for this parasite necessitates direct host-parasite contact during the incubation. Finally, with this experimental model, it is possible to study plants infected by R. lignosus and P. noxius at all the pathogenic stages. [ABSTRACT FROM AUTHOR] more...
- Published
- 1983
23. Growth and mycelial strand production of Rigidoporus lignosus with various nitrogen and carbon sources.
- Author
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Richard, Thierry and Button, Bernard
- Abstract
Growth and differentiation of mycelial strands in Rigidoporus lignosus have been shown to depend on suitable combinations of the pH of the media and the nature of the nitrogen and carbon sources. Amino acids as sole nitrogen sources gave rise to vegetative mycelium. At pH 4.5, growth and mycelial strand differentiation required asparagine, as the fungus failed to grow in the absence of this amino acid. However, at pH 6, differentiation of strands occurred appreciably in asparagine-deficient media, suggesting a close balance between pH and amino acid requirements. Ammonium was required for strand differentiation, while nitrate, as a sole nitrogen source, maintained the fungus undifferentiated. Of the carbohydrates tested, only glucose, fructose and mannose supported strand differentiation. Starch was found to be particularly effective in promoting growth of vegetative mycelium. Strand differentiation required more specific conditions than growth of the vegetative mycelium. [ABSTRACT FROM AUTHOR] more...
- Published
- 1996
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24. Laccases of Rigidoporus lignosus and Phellinus noxius.
- Author
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Geiger, J., Rio, B., Nandris, D., and Nicole, M.
- Abstract
Three phenol oxidases, of which two are excreted by Rigidoporus lignosus and one by Phellinus noxius, have been isolated and purified from culture filtrates. Based on their substrate specificities and spectral characteristics, these enzymes are p-diphenol : oxygen oxidoreductases (laccases; EC 1.10.3.2). A number of their physicochemical properties have been determined. The fact that the two parasites excrete laccases indicates that they belong to the group of lignin degrading (white rot) fungi. Laccase L1 from R. lignosus was purified to homogeneity. [ABSTRACT FROM AUTHOR] more...
- Published
- 1987
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25. EKSPLORASI DAN IDENTIFIKASI Trichoderma spp. DI UNIVERSITAS TEUKU UMAR
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Lola Adres Yanti and Miru Ajro Love Frianos
- Subjects
Rhizoctonia solani ,Horticulture ,Sclerotium ,biology ,Fusarium oxysporum ,Rigidoporus lignosus ,biology.organism_classification ,Trichoderma spp ,Trichoderma sp ,Microscopic observation - Published
- 2018
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26. Determination of White Root Infected Rubber Tree through Dielectric Characteristic in Latex
- Author
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Mohd Suhaimi Sulaiman, Noor Aishah Khairuzzaman, Nur Baiti Wahid, Ahmad Faiz Mohd Sampian, Hadzli Hashim, and Azrie Faris Mohd Azmi
- Subjects
Veterinary medicine ,Materials science ,Natural rubber ,visual_art ,General Engineering ,Analytical chemistry ,visual_art.visual_art_medium ,Root disease ,Statistical analysis ,Dielectric ,Rigidoporus lignosus ,Dielectric spectroscopy - Abstract
White root disease caused byRigidoporus lignosusis one of most serious disease in rubber plantation. Until now, research about white root disease relates to electrical properties is still less. Hence, this research investigated the differences in electrical properties of healthy and white root disease through dielectric constant value. Seventy six samples of healthy and white root disease respectively were collected from rubber estate located in Kota Tinggi, Johor. Measurement of dielectric constant value for both conditions of latex has been done using Electrochemical Impedance Spectroscopy (EIS) in the range of frequency between 50 Hz up to 10 kHz. The result obtained from this measurement was proceeding with the statistical analysis to analyze the differences of healthy and white root disease. It was found that at frequency 150 Hz, the dielectric constant of healthy and white root disease have show significant differences. more...
- Published
- 2015
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27. Biological control of Rigidoporus lignosus in Hevea brasiliensis in Nigeria
- Author
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Abraham Ikponmwosa Ogboghodo, Adefunke Temitayo Adekunle, Nicholas Obehi Ogbebor, and Odeh Nosakhare Eghafona
- Subjects
Veterinary medicine ,Time Factors ,Hypocrea ,Biological pest control ,Nigeria ,Plant Development ,Antibiosis ,Botany ,Genetics ,Root rot ,Rigidoporus lignosus ,Pest Control, Biological ,Ecology, Evolution, Behavior and Systematics ,Plant Diseases ,Trichoderma ,biology ,Inoculation ,biology.organism_classification ,Survival Analysis ,Hypocrea virens ,Infectious Diseases ,Hevea ,Hevea brasiliensis ,Coriolaceae - Abstract
The study was conducted to evaluate in vitro and in vivo control of fungal antagonists on Rigidoporus lignosus (klotzsch) Imaz in Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. in the rain forest zone of Nigeria. In vitro assessments of biological agents were carried out in dixenic cultures in Petri plates. In vivo tests were carried out in the nursery using Hypocrea virens and Hypocrea jecorina. Significant differences were observed with the dual inoculations of fungal antagonists and R. lignosus inoculated the same day, and the antagonists inoculated 24 h before inoculation of R. lignosus. Hypocrea jecorina was most effective in the control of R. lignosus with percentage inhibition of 86.83 %. Hypocrea virens, H. jecorina, Trichoderma spirale, Trichoderma sp., Trichoderma sp. Pers, and Hypocrea lixii were effective on R. lignosus. Fungal antagonists inoculated 24 h before inoculation of R. lignosus exhibited higher inhibitory efficacies than antagonists and R. lignosus inoculated the same day. In the in vivo evaluation of fungi antagonists on R. lignosus, seedlings in H. jecorina treatment at 60 d and H. virens treatment at 150 d after inoculations respectively had the highest plant heaths (highest length of stem and length of tap rot, lowest plant death, and least foliar symptom) compared to the control. Mortality rate was higher at 60 d after inoculation and decline from the third months onward. Significant differences were observed between the control and R. lignosus treatment in all the parameters evaluated. more...
- Published
- 2015
- Full Text
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28. EVALUASI KOMBINASI ISOLAT TRICHODERMA MIKOPARASIT DALAM MENGENDALIKAN PENYAKIT AKAR PUTIH PADA BIBIT KARET
- Author
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Suwandi Suwandi
- Subjects
biology ,Inoculation ,Trichoderma virens ,Biological pest control ,Root disease ,technology, industry, and agriculture ,food and beverages ,lcsh:Plant culture ,biology.organism_classification ,complex mixtures ,Lignosus ,Microbiology ,Horticulture ,Disease severity ,Trichoderma ,lcsh:SB1-1110 ,Rigidoporus lignosus - Abstract
Evaluation of mycoparasitic Trichoderma isolate mixtures to control white root disease on rubber seedlings. Eight isolates of mycoparasitic Trichoderma, as single cultures or in isolate mixtures were tested for their biocontrol efficacy against rubber seedlings inoculated with one of three strains of Rigidoporus lignosus. Biocontrol efficacy of isolates was varied, but not significantly affected by strains of R. lignosus. Mixtures of four isolates were significantly (P < 0.05) reduced the disease severity and percentage of root necrotic as compared to mixtures of two isolates as well as single isolates. The highest disease suppression (65% relative to control) and reduction of inocula on rubber wood sticks (91% relative to control) was achieved in four isolate mixtures of Trichoderma virens (T1+T4+ T9+ T11). more...
- Published
- 2011
29. Efektivitas Fungisida Berbahan Aktif Heksakonazol terhadap Penyakit Jamur Akar Putih Bibit Tanaman Karet (Hevea brasiliensis)
- Author
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Ingrid Ovie Sitompul, Mardiana Wahyuni, and Jenny Hotnida Simanjuntak
- Subjects
Fungicide ,Horticulture ,chemistry.chemical_compound ,biology ,chemistry ,Applied Mathematics ,Randomized block design ,Root disease ,Hexaconazole ,Hevea brasiliensis ,Tree density ,Rigidoporus lignosus ,biology.organism_classification - Abstract
White Root Fungus Disease (JAP) is the biggest threat in rubber. The attack can result in a significant reduction in number, especially in plants lasting 0-3 years. The pathogen that causes white fungus is Rigidoporus lignosus. In JAP endemic areas, decreased tree density 40-50%. In rubber seedlings, symptoms of JAP attack with yellowing color leaves, then dry and fall. This study was conducted at STIP-AP Medan from January 2017 to June 2017. The aim of the study was to determine the effectiveness of several doses of hexaconazole. This study uses a non-factorial Randomized Block Design (RBD) with four replications. Hexaconazole fungicides are active with treatment of A0, A1, A2, A3 and A4 (0, 5, 10, 15 and 20 ml / l air). The results of this study indicate that A2 application of hexaconazole-active fungicides (10 ml / l air) has been effective in controlling JAP disease in rubber seedlings. more...
- Published
- 2018
- Full Text
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30. THE CORELATIONS BETWEEN WHITE ROT (RIGIDOPORUS LIGNOSUS L.) INCIDENCE AND SOIL CHARACTERS OF RUBBER ECOSYSTEM IN PENUMANGAN BARU, LAMPUNG
- Author
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Titik Nur Aeny, Joko Prasetyo, and Radix Suharjo
- Subjects
technology, industry, and agriculture ,Baru ,food and beverages ,macromolecular substances ,lcsh:Plant culture ,Biology ,biology.organism_classification ,complex mixtures ,Indicator plant ,Horticulture ,Fungal Diversity ,Rigidoporus lignosus, rubber, soil character ,Soil pH ,White rot ,Cation-exchange capacity ,lcsh:SB1-1110 ,Ecosystem ,Rigidoporus lignosus - Abstract
The corelations between White Rot (Rigidoporus lignosus L.) incidence and soil characters of rubber ecosystem in Penumangan Baru, Lampung. A study was conducted to evaluate the corelation between soil characters and white rot incidence. The study was done in Penumangan Lampung from June 2007 to July 2008. Seven sites were observed on the incidence of white rot of indicator plants (cassava). Physical, chemical, and biological soil characters were correlated and regressed with white rot incidence. The results showed that there were correlation between white rot incidence and soil characters such as pH KCl, number of fungal genus (fungal diversity), Ca+, total cation, CEC, and saturation base. Ion Ca+, total cation, saturation base correlated positively with white rot incidence, on the contrary, fungal diversity, cation exchange capacity, and pH KCl correlated negatively with white rot incidence. Thus, efforts to increase fungal diversity, cation exchange capacity, and pH (in acidic soil) could be used as basic strategies to develope intergrated control of white rot disease. more...
- Published
- 2009
- Full Text
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31. Laccase-based biosensors for detection of phenolic compounds
- Author
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Damià Barceló, Roberto Parra, Sergio O. Martinez-Chapa, José Manuel Rodríguez-Delgado, Melissa Marlene Rodríguez-Delgado, Gibrán S. Alemán-Nava, and Graciano Dieck-Assad
- Subjects
Aspergillus oryzae ,Amperometry ,Laccases ,Review ,laccase ,Analytical Chemistry ,amperometric biosensor ,Trametes ,Electrochemical transducers ,conductometric biosensor ,Toxicodendron vernicifluum ,sensor ,Cerrena unicolor ,Organic chemistry ,Bacteriophages ,Aliments -- Anàlisi ,comparative study ,Spectroscopy ,thermal sensor ,limit of detection ,Polyporus pinsitus ,biology ,optical biosensor ,Chemistry ,fungus ,Enzymes ,Electrochemical transducer ,priority journal ,potentiometric biosensor ,Aspergillus niger ,fluorescence ,Myceliophthora thermophila ,Fenols ,Medical applications ,lifespan ,conductometry ,Conductometry ,phenol derivative ,Ganoderma lucidium ,Transducers ,Optical transducer ,Trametes versicolor ,Rigidoporus lignosus ,macromolecular substances ,Trametes hirsuta ,voltammetric biosensor ,Optical transducers ,biosensor ,Absorció ,Absorption ,Transduction ,Phenols ,Environmental Chemistry ,response time ,Detection limit ,Laccase ,Chromatography ,nonhuman ,Phenol ,Fungi ,technology, industry, and agriculture ,Ganoderma ,Pleurotus ostreatus ,biology.organism_classification ,Biosensors ,7 INGENIERÍA Y TECNOLOGÍA ,potentiometry ,immobilization ,Biosensor ,Food -- Analysis - Abstract
Monitoring of phenolic compounds in the food industry and for environmental and medical applications has become more relevant in recent years. Conventional methods for detection and quantification of these compounds, such as spectrophotometry and chromatography, are time consuming and expensive. However, laccase biosensors represent a fast method for on-line and in situ monitoring of these compounds. We discuss the main transduction principles. We divide the electrochemical principle into amperometric, voltammetric, potentiometric and conductometric sensors. We divide optical transducers into fluorescence and absorption. The amperometric transducer method is the most widely studied and used for laccase biosensors. Optical biosensors present higher sensitivity than the other biosensors. Laccase production is dominated by a few fungus genera: Trametes, Aspergillus, and Ganoderma. We present an overview of laccase biosensors used for the determination of phenolic compounds in industrial applications. © 2015 The Authors. more...
- Published
- 2015
32. Degradation of Polycyclic Aromatic Hydrocarbons by Rigidoporus lignosus and its Laccase in the Presence of Redox Mediators
- Author
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Cambria, M. T., Minniti, Z., Librando, V., and Cambria, A.
- Published
- 2008
- Full Text
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33. Distribution of Rigidoporus lignosus genotypes in a rubber plantation, as revealed by somatic compatibility
- Author
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Harman Hamidson, Suwandi, and Shigeo Naito
- Subjects
Veterinary medicine ,animal structures ,Somatic cell ,Root disease ,Fungus ,Biology ,biology.organism_classification ,digestive system ,Lignosus ,body regions ,surgical procedures, operative ,Natural rubber ,visual_art ,Botany ,Genotype ,visual_art.visual_art_medium ,Rigidoporus lignosus ,Ecology, Evolution, Behavior and Systematics ,Clonal growth - Abstract
A study was conducted to determine the spatial distribution of somatic incompatibility groups (SIGs) in Rigidoporus lignosus populations responsible for white root disease in a south Sumatera rubber plantation. Most isolates colonizing the stumps were somatically compatible with isolates from either a nearby stump or a living rubber tree, which suggested evidence of stump-to-tree or stump-to-stump clonal growth of the fungus. SIGs of R. lignosus occupied an area territorially in which the minimum size estimate for the largest genotype was 25 m in length. more...
- Published
- 2004
- Full Text
- View/download PDF
34. Comparative study for detecting Rigidoporus lignosus on rubber trees
- Author
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Albert Flori and Jean Guyot
- Subjects
Early detection ,Rigidoporus lignosus ,Biology ,Paillage ,Méthode de lutte ,Natural rubber ,Root rot ,Sampling density ,H20 - Maladies des plantes ,U10 - Informatique, mathématiques et statistiques ,Expérimentation au champ ,Contrôle de maladies ,Lutte culturale ,Sampling (statistics) ,Rigidoporus microporus ,biology.organism_classification ,Pourriture des racines ,Hevea brasiliensis ,Horticulture ,visual_art ,visual_art.visual_art_medium ,Agronomy and Crop Science ,Mulch ,Espacement - Abstract
Early detection of root diseases is a great problem in perennial crops, especially in rubber tree. Planters are continually confronted with the balance of efficiency/cost of the detection. Therefore, a trial was set up to compare three sampling densities and four exposure techniques for detection of white root rot in rubber plantings. A complete census of all trees is the only efficient method of detection in a control program. When reducing the sampling density, a lot of infected trees are not detected and they remain an inoculum source for the spread of the disease. Mulching for one month followed by root exposure is the best technique to reveal the presence of the fungus. The sampling density is of greater significance than the infection exposure method. more...
- Published
- 2002
- Full Text
- View/download PDF
35. Distribution of Rigidoporus lignosus genotypes in a rubber plantation, as revealed by somatic compatibility
- Author
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Hamidson, Suwandi Harman and Naito, Shigeo
- Published
- 2004
- Full Text
- View/download PDF
36. Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus lignosus
- Author
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MariaTeresa Cambria, Santa Ragusa, Antonio Cambria, and Enrico Rizzarelli
- Subjects
Laccase ,chemistry.chemical_classification ,Sequence Homology, Amino Acid ,Chemistry ,Basidiomycota ,Molecular Sequence Data ,Culture Media ,Fungal Proteins ,Kinetics ,Enzyme ,Biochemistry ,Sequence Analysis, Protein ,Extracellular ,Substrate specificity ,Electrophoresis, Polyacrylamide Gel ,Amino Acid Sequence ,Isoelectric Focusing ,Rigidoporus lignosus ,Oxidoreductases ,Copper ,Biotechnology - Abstract
Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications. more...
- Published
- 2000
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37. Recombinant laccase: II. Medical biosensor
- Author
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Tercio Bezerra Correia Terencio, Claudio Nicolini, Eugenia Pechkova, D. Scudieri, Manuela Adami, and Nicola Luigi Bragazzi
- Subjects
Potentiometric titration ,Rigidoporus lignosus ,Biosensing Techniques ,Microscopy, Atomic Force ,Absorbance ,Ultraviolet visible spectroscopy ,Genetics ,Humans ,Enzyme kinetics ,Molecular Biology ,Electrodes ,Chemistry ,Laccase ,Chronoamperometry ,cyclic voltammetry ,Amperometry ,Recombinant Proteins ,chronoamperometry ,Kinetics ,Biochemistry ,UV spectrophotometry ,laccase ,clomipramine ,Clomipramine ,Potentiometry ,Cyclic voltammetry ,Biosensor ,Nuclear chemistry - Abstract
Langmuir-Blodgett (LB) technology was used to build a high-sensitivity enzyme-based biosensor for medical purposes. Recombinant fungal laccase from Rigidoporous lignosus, as previously described, was used to catalyze a widely used antidepressant in a micromolar range, namely, clomipramine. The topological properties of the laccase thin film were characterized via LB π-A isotherm and AFM (mean roughness 8.22 nm, compressibility coefficient 37.5 m/N). The sensitivity of the biosensor was investigated via UV spectroscopy, and linearity was found in the absorbance peak shift at 400 nm at drug concentration varying up to 20 uM. The enzyme kinetics was subsequently investigated with potentiometric and amperometric measurements, and we found electronic transfer of at least 1 electron, k(s) 0.57 s(-1), diffusion coefficient 3 × 10(-6) cm(2)/s, K(cat) 6825.92 min(-1), K(M) 4.1 uM, K(cat)/K(M) 2.8 × 10(7) mol(-1) s(-1), sensitivity of 440 nA/uM, maximum velocity 1706.48 nA/s, and response time less than 5 s. The amperometric and potentiometric measurements were repeated after a month, confirming the stability of the biosensor. more...
- Published
- 2012
38. In vitro characterization andin vivo detection ofRigidoporus lignosus, the causal agent of white root disease inHevea brasiliensis, by ELISA techniques
- Author
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Louanchi, M., Robin, P., Michels, T., Balesdent, M. H., and Despréaux, D.
- Published
- 1996
- Full Text
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39. New developments in chemical control of white root disease of Hevea brasiliensis in Africa
- Author
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Meriem Louanchi, Eric Gohet, Tran Van Canh, Denis Despréaux, ProdInra, Migration, Station de pathologie végétale, and Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,[SDV]Life Sciences [q-bio] ,Root disease ,Champignon ,Rigidoporus lignosus ,01 natural sciences ,Maladie des plantes ,ComputingMilieux_MISCELLANEOUS ,H20 - Maladies des plantes ,Pourriture ,biology ,Contrôle de maladies ,Cyproconazole ,Forestry ,biology.organism_classification ,Disease control ,3. Good health ,[SDV] Life Sciences [q-bio] ,Hevea brasiliensis ,010602 entomology ,Horticulture ,Fongicide ,Chemical control ,Agronomy and Crop Science ,Racine ,010606 plant biology & botany - Abstract
Les trois principaux parasites des racines d'Hevea brasiliensis en Afrique sont Rigidoporus lignosus, Phellinus noxius et Armillaria sp.. Seul Rigidoporus lignosus, qui est responsable de la pourriture blanche des racines est présent dans les zones hévéicoles et reste la cause principale de mortalité des arbres en Afrique. De nouveaux fongicides ont été testés afin d'améliorer la lutte contre la pourriture blanche des racines causée par Rigidoporus lignosus. Certains composés à base de triazole se sont révélés être très efficaces in vitro et sur des expérimentations à petite échelle avec des seedlings ou des stumps placés en sols infestés artificiellement. Deux applications tous les 6 mois de fongicide sous forme liquide (Alto, Sandoz) et des applications de triadimenol sous forme granulaire (Bayfidan, Bayer) à raison de 0,5 g a.i. par arbre ont donné de bons résultats au champ. Leur utilisation est recommandée dans la pratique. (traduction du résumé d'auteur) more...
- Published
- 1991
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40. Robert napper — Pioneer of root disease control in Hevea rubber
- Author
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Paul Holliday
- Subjects
Horticulture ,Natural rubber ,visual_art ,visual_art.visual_art_medium ,Root disease ,Plant Science ,Rigidoporus lignosus ,Biology ,biology.organism_classification ,Disease control ,Hevea - Published
- 1996
- Full Text
- View/download PDF
41. Rapport de mission au Ghana à la GREL du 15 au 21 novembre 1999
- Author
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Guyot, Jean
- Subjects
Manihot esculenta ,Glomerella cingulata ,Recherche ,Rigidoporus lignosus ,Isoptera ,Loranthaceae ,Méthode de lutte ,Fomes ,Culture en mélange ,H20 - Maladies des plantes ,Expérimentation au champ ,Contrôle de maladies ,H10 - Ravageurs des plantes ,Hevea brasiliensis ,Fongicide - Abstract
L'objectif de la mission est d'analyser les procédures actuelles mises en oeuvre pour la lutte contre les maladies de l'hévéa en plantations villageoises et de proposer des recommandations et/ou la mise en place d'expérimentations dans le cadre du volet recherche d'accompagnement de la deuxième phase du projet villageois. Concrètement, il consiste à : - prendre connaissance des problèmes sanitaires rencontrés en plantations villageoises et des procédures de lutte actuellement mises en oeuvre, - étudier la pertinence de ces procédures et éventuellement d'y apporter des modifications, - informer les encadreurs du projet villageois de l'état d'avancement de la recherche dans le domaine de la protection sanitaire de l'hévéa et introduire si nécessaire de nouvelles techniques, - proposer des expérimentations permettant de répondre aux questions spécifiques posées par l'hévéaculture villageoise ghanéenne. Les responsables de l'encadrement de la GREL ont soulevé les problèmes sanitaires suivants, dans l'ordre de leurs proccupations : - révision des procédures de lutte contre lFomes avec mise en place éventuelle d'expérimentations : fongicides efficaces, fréquence, durée, et période d'application des fongicides, nécessité d'isolement total ou partiel des arbres infectés, situation actuelle des recherches sur la lutte génétique et biologique, - lutte contre les termites au planting, - lutte contre les maladies de feuilles en pépinières, - recommandations concernant l'association hévéa-manioc, - recommandations concernant les Loranthaceae. more...
- Published
- 1999
42. Report on the mission to Ghana, GREL, 15 to 21 November 1999
- Author
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Guyot, Jean
- Subjects
Manihot esculenta ,Glomerella cingulata ,Recherche ,Rigidoporus lignosus ,Isoptera ,Loranthaceae ,Méthode de lutte ,Fomes ,Culture en mélange ,H20 - Maladies des plantes ,Expérimentation au champ ,Contrôle de maladies ,H10 - Ravageurs des plantes ,Hevea brasiliensis ,Fongicide - Abstract
The purpose of this mission was to analyse the procedures currently applied for controlling rubber tree diseases on smallholdings, then propose recommendations and/or set up trials under the adaptive research component of the second phase of the smallholdeer project. In substance, this consisted in: - ascertaining the phytosanitaiy problems encountered on smallholdings and the control procedures currently implemented, - studying the appropriateness of those procedures and possibly make modifications, - informing smallholder project supervisors of the advances made in research on the phytosanitaiy protection of rubber trees, and introducing new techniques if necessary, - proposing trials providing answers to specific questions raised by smallholder rubber cultivation in Ghana. GREL supervisory staff mentioned the following phytosanitary problems, in order of importance to them: - revision of Fomes control procedures, possibly setting up trials: effective fungicides, application frequency, duration and period of fungicide applications, need for partial or total isolation of infected trees, current state of research in terms of genetic and biological control, - termite control at the time of planting, - control of leaf diseases in the nursery, - recommendations on rubber-cassava intercropping, - recommendations regarding Loranthaceae . more...
- Published
- 1999
43. Le Fomes de l'hévéa à SOCFINDO (Indonésie). Incidence. Méthode de lutte. Recommandations. Rapport de mission 12-20 mai 1997
- Author
-
Renard, Jean-Luc
- Subjects
Plantations ,Botryodiplodia ,Rigidoporus lignosus ,Trichoderma koningii ,Maladie des plantes ,Fomes ,Surveillance épidémiologique ,Elaeis guineensis ,Corticium ,H20 - Maladies des plantes ,Lutte chimique ,Coopération internationale ,Contrôle de maladies ,Ganoderma ,Lutte biologique ,Hevea brasiliensis - Abstract
L'objectif principal de la mission a été de faire le point sur le problème du Fomes (Rigidoporus lignosus) de l'hévéa dans différentes plantations de la SOCFINDO. Cette visite constitue une reprise de la coopération conduite de 1987 à 1993 avec SOCFINDO sur le problème du Fomes. Le présent rapport fait le bilan détaillé d'informations recueillies au cours de la mission. Les recommandations du rapport sont destinées à améliorer les connaissances sur le Fomes et les méthodes de lutte. Bien que concentrées sur le Fomes, ces visites ont également été l'occasion d'analyser d'autres problèmes tel que : le Corticium salmonicolor, le Botryodiplodia , le Bark Necrosis more...
- Published
- 1997
44. Lutte contre le pourridié blanc (Fomès) de l'hévéa
- Author
-
Tran Van Canh, Canh
- Subjects
Lutte chimique ,Contrôle de maladies ,Dose d'application ,Champignon ,Lutte physique ,Rigidoporus lignosus ,Agent pathogène ,Hevea brasiliensis ,Maladie des plantes ,Fomes ,Fongicide ,Surveillance épidémiologique ,H20 - Maladies des plantes - Abstract
Le pourridié blanc des racines de l'hévéa est dû à un champignon, le fomès (Rigidoporus lignosus), parasite important qui affecte presque toutes les zones hévéicoles. Chaque souche que le champignon colonise constitue un foyer d'infection qui menace les hévéas plantés dans un rayon parfois supérieur à 40-50 mètres. Un arbre infecté est tué par la nécrose de son système racinaire. Une lutte préventive est donc préconisée avant le planting, et une lutte directe en parcelle est possible en cas d'infection de la plantation more...
- Published
- 1997
45. Rapport de mission au Gabon. Phytopathologie. Du 09 au 23 janvier 1996
- Author
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Pinard, Fabrice
- Subjects
Glomerella cingulata ,Rigidoporus lignosus ,Lutte génétique ,Défoliation ,Projet de recherche ,Maladie des plantes ,Anthracnose ,Armillaria mellea ,H20 - Maladies des plantes ,Plantation ,Lutte chimique ,Contrôle de maladies ,Maladie fongique ,Armillaria ,Hevea brasiliensis - Abstract
Au Gabon sur les plantations d'hévéas HEVEGAB les infections foliaires (anthracnose) dues à Colletotrichum gloesporioides et les maladies des racines causées par Rigidoporus lignosus et Armilleria heimii (A. mellea) imposent un suivi sanitaire constant. Dans la première partie le rapport donne le bilan des défoliations artificielles par épandage aérien effectué en 1995 ainsi que le programme de travail de 1996 préparé avec le CATH/HEVEGAB puis il aborde la mise en place d'une lutte contre les maladies des racines selon qu'elle s'adresse au milieu villageois ou à la plantation industrielle. Des actions ponctuelles sont préconisées pour réduire les attaques de maladies de panneaux observées sur PB 260 et envisager la lutte contre les épiphytes (Poranthacées). La deuxième partie du rapport porte sur les travaux effectués sur l'agent de l'anthracnose foliaire de l'hévéa. Les deux principaux axes fixés par le programme de recherche sont l'amélioration de la gestion et de l'efficacité de la lutte par défoliation artificielle ainsi que la préparation d'une lutte génétique more...
- Published
- 1996
46. Contribution à l'étude de la lectine du champignon basidiomycète rigidoporus lignosus : purification, propriétés physico-chimiques et localisation de la lectine et de ses sites d'affinité
- Author
-
Richard, Thierry, Université Henri Poincaré - Nancy 1 (UHP), Université Henri Poincaré - Nancy 1, Bernard Botton, and UL, Thèses
- Subjects
Basidiomycètes ,Hévéa brasiliensis ,Mycélium ,Lectine ,Relation hôte agent ,Lectines ,Récepteur biologique ,Rigidoporus lignosus ,Localisation ,Immunocytochimie ,Hyphe ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,Étude en laboratoire ,Purification ,Propriété physicochimique ,Racine ,Reconnaissance - Abstract
Not available, Rigidoporus lignosus est un basidiomycète, agent du pourridié blanc de nombreuses espèces tropicales telle qu'Hévéa brasiliensis. Ce champignon différencie des organes agrégés appelés palmettes à partir du mycélium végétatif. Ces organes sont impliqués dans la propagation du champignon dans le sol et dans l'infection des racines des arbres. Chez ce champignon une lectine a été purifiée 64 fois avec un rendement de 43%. C’est une glycoprotéine ayant une masse moléculaire de 150 kDa, composée de 4 sous-unités identiques de 35 kDa et de points isoélectriques différents. La lectine agglutine de façon préférentielle les hématies canines ainsi que les hématies humaines du groupe O. le L-fucose est le sucre qui présente la meilleure affinité pour la lectine. La molécule est très stable lors des variations de ph et de température, ce qui implique que sa liaison avec le récepteur fait vraisemblablement intervenir des liaisons de type hydrophobe. L’utilisation de la lectine complexée à l'or colloïdal a permis de localiser les sites récepteurs à la surface pariétale des hyphes fongiques ainsi qu'à la surface cellulaire des racines d'Hévéa brasiliensis. L’obtention d'anticorps anti-lectine a permis de localiser cette glycoprotéine au niveau du cytoplasme des hyphes du mycélium végétatif et à la surface des hyphes des palmettes. Cette lectine semble donc pouvoir jouer un rôle non seulement dans l'interaction entre les hyphes lors de la formation des palmettes mais aussi dans les processus de reconnaissance et d'infection de la plante more...
- Published
- 1995
47. Contribution to the study of a lectin from the basidiomycete fungus rigidoporus lignosus : purification, physico-chemical properties and localization of the protein and of the affinity sites
- Author
-
Richard, Thierry, UL, Thèses, Université Henri Poincaré - Nancy 1 (UHP), Université Henri Poincaré - Nancy 1, and Bernard Botton
- Subjects
Basidiomycètes ,Hévéa brasiliensis ,Mycélium ,Lectine ,Relation hôte agent ,Lectines ,Récepteur biologique ,Rigidoporus lignosus ,Localisation ,Immunocytochimie ,Hyphe ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,Étude en laboratoire ,Purification ,Propriété physicochimique ,Racine ,Reconnaissance - Abstract
Not available, Rigidoporus lignosus est un basidiomycète, agent du pourridié blanc de nombreuses espèces tropicales telle qu'Hévéa brasiliensis. Ce champignon différencie des organes agrégés appelés palmettes à partir du mycélium végétatif. Ces organes sont impliqués dans la propagation du champignon dans le sol et dans l'infection des racines des arbres. Chez ce champignon une lectine a été purifiée 64 fois avec un rendement de 43%. C’est une glycoprotéine ayant une masse moléculaire de 150 kDa, composée de 4 sous-unités identiques de 35 kDa et de points isoélectriques différents. La lectine agglutine de façon préférentielle les hématies canines ainsi que les hématies humaines du groupe O. le L-fucose est le sucre qui présente la meilleure affinité pour la lectine. La molécule est très stable lors des variations de ph et de température, ce qui implique que sa liaison avec le récepteur fait vraisemblablement intervenir des liaisons de type hydrophobe. L’utilisation de la lectine complexée à l'or colloïdal a permis de localiser les sites récepteurs à la surface pariétale des hyphes fongiques ainsi qu'à la surface cellulaire des racines d'Hévéa brasiliensis. L’obtention d'anticorps anti-lectine a permis de localiser cette glycoprotéine au niveau du cytoplasme des hyphes du mycélium végétatif et à la surface des hyphes des palmettes. Cette lectine semble donc pouvoir jouer un rôle non seulement dans l'interaction entre les hyphes lors de la formation des palmettes mais aussi dans les processus de reconnaissance et d'infection de la plante more...
- Published
- 1995
48. Lutte contre le pourridié blanc (Fomes) de l'hévéa
- Author
-
Tran Van Canh, Canh and Tran Van Canh, Canh
- Abstract
Dans les plantations, le pourridié blanc des racines de l'hévéa, dû à Rigidoporus lignosus, se transmet d'arbre en arbre par l'intermédiaire des racines latérales. Pour obtenir de bons résultats dans la lutte contre ce parasite, quatre opérations sont préconisées : la détection des foyers d'infection, le marquage des arbres à traiter, le traitement et la neutralisation des sources d'infection. Ces conseils techniques sont à renouveler chaque année pendant au moins trois ans more...
- Published
- 1996
49. Lutte contre la pourriture blanche des racines de l'hévéa due au Rigidoporus lignosus
- Author
-
Tran Van Canh, Canh
- Subjects
Lutte chimique ,Contrôle de maladies ,Rigidoporus lignosus ,Maladie fongique ,Hevea brasiliensis ,Fongicide ,H20 - Maladies des plantes - Abstract
La pourriture blanche des racines d'Hévéa due au Rigidoporus lignosus (K.L.) est considérée comme une des plus importantes maladies de l'Hévéa dans la zone forestière en Afrique. Cette maladie est contrôlée par des applications tous les six mois des fongicides : Alto (Cyproconazole) ou Bayfidan (Triadimenol) et par des isolations des sources d'infection. Des tests d'efficacité de nouveaux fongicides contre ce parasite par la méthode d'inoculation artificielle ont montré une très bonne efficacité des fongicides suivants : Atémi S (Cyproconazole+Soufre), Anvil (Hexaconazole), Sumi 8 (Diniconazole), et Vectra (Bromuconazole). Les résultats des essais en conditions naturelles depuis trois ans ont permis de recommander ces nouveaux fongicides dans la lutte contre cette maladie more...
- Published
- 1994
50. Rapport de mission au Cameroun du 9 au 23 mars 1994
- Author
-
Pinard, Fabrice
- Subjects
Glomerella cingulata ,Épiphyte ,Épandage aérien ,Rigidoporus lignosus ,H60 - Mauvaises herbes et désherbage ,Loranthaceae ,Maladie des plantes ,Corynespora cassiicola ,H20 - Maladies des plantes ,Lutte chimique ,Projet de développement ,Litière végétale ,Contrôle de maladies ,Hevea brasiliensis ,Fongicide ,Action systémique - Abstract
Visite de la plantation HEVECAM de La Niété : situation phytosanitaire et politique de lutte contre les maladies foliaires, racinaires et le développement de Loranthacées (épiphytes). Les observations sur les travaux en cours ont permis de faire le point sur les maladies foliaires dues à Corynespora gloeosporioïdes et C. cassiicola, le pourridié blanc des racines dû à Rigidoporus lignosus et d'évaluer les essais de lutte chimique (applications aériennes de fongicides, traitement des litières, empoisonnement des souches). Evaluation de l'impact de R. lignosus sur la production. Propositions de développement de travaux de recherches. Visites des stations d'Ekona et de N'kolbisson. Projet de renforcement de la recherche hévéicole à l'IRA. En annexe, programme de travail sur C. cassiicola de Marc Lefeuvre more...
- Published
- 1994
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