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Abstract Leafcutter ants are dominant herbivores in the Neotropics and rely on a fungus (Leucoagaricus gongylophorus) to transform freshly gathered leaves into a source of nourishment rather than consuming the vegetation directly. Here we report two virus-like particles that were isolated from L. gongylophorus and observed using transmission electron microscopy. RNA sequencing identified two +ssRNA mycovirus strains, Leucoagaricus gongylophorus tymo-like virus 1 (LgTlV1) and Leucoagaricus gongylophorus magoulivirus 1 (LgMV1). Genome annotation of LgTlV1 (7401 nt) showed conserved domains for methyltransferase, endopeptidase, viral RNA helicase, and RNA-dependent RNA polymerase (RdRp). The smaller genome of LgMV1 (2636 nt) contains one open reading frame encoding an RdRp. While we hypothesize these mycoviruses function as symbionts in leafcutter farming systems, further study will be needed to test whether they are mutualists, commensals, or parasites.

Our study identified strains of the A/H5N1 virus in analyzed samples of subsistence poultry, wild birds, and mammals, belonging to clade 2.3.4.4b, genotype B3.2, with very high genetic similarity to strains from Chile, Uruguay, and Argentina. This suggests a migratory route for wild birds across the Pacific, explaining the phylogenetic relatedness. The Brazilian samples displayed similarity to strains that had already been previously detected in South America. Phylogeographic analysis suggests transmission of US viruses from Europe and Asia, co-circulating with other lineages in the American continent. As mutations can influence virulence and host specificity, genomic surveillance is essential to detect those changes, especially in critical regions, such as hot spots in the HA, NA, and PB2 sequences. Mutations in the PB2 gene (D701N and Q591K) associated with adaptation and transmission in mammals were detected suggesting a potential zoonotic risk. Nonetheless, resistance to neuraminidase inhibitors (NAIs) was not identified, however, continued surveillance is crucial to detect potential resistance. Our study also mapped the spread of the virus in the Southern hemisphere, identifying possible entry routes and highlighting the importance of surveillance to prevent outbreaks and protect both human and animal populations.

Theobroma cacao plantations are of significant economic importance worldwide, primarily for chocolate production. During the harvest and processing of cocoa beans, they are subjected to fermentation either by microorganisms present in the environment (spontaneous fermentation) or the addition of starter cultures, with different strains directly contributing distinct flavor and color characteristics to the beans. In addition to fungi and bacteria, viruses are ubiquitous and can affect the quality of the fermentation process by infecting fermenting organisms, destabilizing microbial diversity, and consequently affecting fermentation quality. Therefore, in this study, we explored publicly available metatranscriptomic libraries of cocoa bean fermentation in Limon Province, Costa Rica, looking for viruses associated with fermenting microorganisms. Libraries were derived from the same sample at different time points: 7, 20, and 68 h of fermentation, corresponding to yeast- and lactic acid bacteria-driven phases. Using a comprehensive pipeline, we identified 68 viral sequences that could be assigned to 62 new viral species and 6 known viruses distributed among at least nine families, with particular abundance of elements from the Lenarviricota phylum. Interestingly, 44 of these sequences were specifically associated with ssRNA phages (Fiersviridae) and mostly fungi-infecting viral families (Botourmiaviridae, Narnaviridae, and Mitoviridae). Of note, viruses from those families show a complex evolutionary relationship, transitioning from infecting bacteria to infecting fungi. We also identified 10 and 3 viruses classified within the Totiviridae and Nodaviridae families, respectively. The quantification of the virus-derived RNAs shows a general pattern of decline, similar to the dynamic profile of some microorganism genera during the fermentation process. Unexpectedly, we identified narnavirus-related elements that showed similarity to segmented viral species. By exploring the molecular characteristics of these viral sequences and applying Hidden Markov Models, we were capable of associating these additional segments with a specific taxon. In summary, our study elucidates the complex virome associated with the microbial consortia engaged in cocoa bean fermentation that could contribute to organism/strain selection, altering metabolite production and, consequently, affecting the sensory characteristics of cocoa beans.

Weeds pose a significant threat to agricultural production, leading to substantial yield losses and increased herbicide usage, with severe economic and environmental implications. This paper uses deep learning to explore a novel approach via targeted segmentation mapping of crop plants rather than weeds, focusing on canola (Brassica napus) as the target crop. Multiple deep learning architectures (ResNet-18, ResNet-34, and VGG-16) were trained for the pixel-wise segmentation of canola plants in the presence of other plant species, assuming all non-canola plants are weeds. Three distinct datasets (T1_miling, T2_miling, and YC) containing 3799 images of canola plants in varying field conditions alongside other plant species were collected with handheld devices at 1.5 m. The top performing model, ResNet-34, achieved an average precision of 0.84, a recall of 0.87, a Jaccard index (IoU) of 0.77, and a Macro F1 score of 0.85, with some variations between datasets. This approach offers increased feature variety for model learning, making it applicable to the identification of a wide range of weed species growing among canola plants, without the need for separate weed datasets. Furthermore, it highlights the importance of accounting for the growth stage and positioning of plants in field conditions when developing weed detection models. The study contributes to the growing field of precision agriculture and offers a promising alternative strategy for weed detection in diverse field environments, with implications for the development of innovative weed control techniques.

Manioc flour and gum (starch) have a cultural value for Amazonians. The processing of cassava roots releases manipueira, a liquid waste with a high polluting potential, due to its high levels of hydrocyanic acid. The large volumes of manipueira produced in this sector require safe disposal, whether for cooking (tucupi, flour), drinking or agronomic purposes. The aim of this study was to evaluate the stabilization time of chemical attributes and the nutritional quality of manipueira. For this study, cassava roots of the Amazonas cultivar (bitter or wild) harvested 12 months after the manioc was grown, were processed to extract the manipueira, which was stored and sampled at 0; 2; 5; 8; 11; 18; 25; 32; 46; 62 and 92 days. The variables evaluated were: pH and total cyanide content. Nutrients (N, P, K, Ca, Mg and S) were assessed at 0, 47, 62 and 92 days after storage (DAA). The pH stabilized at 10 DAA, varying between 3.8 and 4.0. The total cyanide content stabilized at 62 DAA, containing 410 mg L-1. The macronutrient contents did not vary with storage time, with the following decreasing order of concentration: K > N > P > Mg > Ca > S, corresponding to the contents: 7.49; 1.25; 1.06; 0.68; 0.41 and 0.18 g-1, respectively.

A calagem apresenta impactos positivos na qualidade dos solos, como o aumento do pH e na disponibilidade de nutrientes. No estado de Roraima, solos agricultáveis, em grande parte, estão sob domínios do bioma savana e apresentam pH ácido e distrofia, condicionando o uso agrícola a investimentos na correção da fertilidade. Assim, objetivou-se avaliar diferentes métodos de recomendação de calcário e seus efeitos nas características químicas de três tipos de solo, tendo o milho como planta indicadora em um cultivo em vasos. As menores doses consideraram a neutralização da acidez trocável e as maiores a neutralização da acidez trocável e o aumento dos níveis de cálcio e magnésio. No solo mais argiloso, as diferentes doses de calcário não afetaram a produção inicial de biomassa seca; dessa forma, um método que garanta o fornecimento de cálcio e magnésio para a cultura, além de neutralizar as formas tóxicas de alumínio, é o mais adequado. Nos solos mais arenosos e com baixo poder tampão, predominantes na savana, as maiores doses de calcário reduziram a produção de biomassa. Nesses solos, o método baseado na neutralização do alumínio trocável foi eficiente em elevar o pH e reduzir o Al trocável, mas não garantiu teores de cálcio e magnésio adequados para a necessidade da cultura. Os resultados obtidos evidenciam a importância de uma abordagem individualizada na determinação da necessidade de calagem para os solos de Roraima.

The World Health Organization (WHO) recognizes schistosomiasis as one of the Neglected Tropical Diseases targeted for global elimination in the 2030 Agenda of the Sustainable Development Goals. In Brazil, schistosomiasis mansoni is considered a public health problem, particularly prevalent among vulnerable populations living in areas with poor environmental and sanitary conditions. In 2022, the WHO published a Guideline encompassing recommendations to assist national programs in endemic countries in achieving morbidity control, eliminating schistosomiasis as a public health problem, and advancing towards interrupting transmission. The perspectives presented here, collectively prepared by members of the Oswaldo Cruz Foundation’s (Fiocruz) Schistosomiasis Translational Program (FioSchisto), along with invited experts, examine the feasibility of the WHO recommendations for the Brazilian settings, providing appropriate recommendations for public health policies applicable to the epidemiological reality of Brazil, and suggests future research to address relevant issues. In Brazil, the provision of safe water and sanitation should be the key action to achieve schistosomiasis elimination goals. The agencies involved in measures implementation should act together with the Primary Care teams for planning, executing, monitoring, and evaluating actions in priority municipalities based on their epidemiological indicators. Host snails control should prioritize judicious ecological interventions at breeding sites. The Information, Education, and Communication (IEC) strategy should be associated with water and sanitation and other control actions, actively involving school community. To identify infected carriers, FioSchisto recommends a two-stage approach of immunological and molecular tests to verify transmission interruption during the intervention and beyond. Praziquantel administration should be done under medical supervision at the Primary Care level. MDA should be considered in exceptional settings, as a measure of initial attack strategy in locations presenting high endemicity, always integrated with water and sanitation, IEC, and snail control. To assist decision-making, as well as the monitoring and evaluation of strategic actions, there is a need for an Information System. FioSchisto considers this systematization essential to make investments in strategic research to support the improvement of schistosomiasis control actions. Efforts toward schistosomiasis elimination in Brazil will succeed with a paradigm shift from the vertical prescriptive framework to a community-centered approach involving intersectoral and interdisciplinary collaboration.

A educação para inclusão de pessoas com necessidades educacionais especiais no ensino básico, no Brasil, tem sido um grande desafio nos tempos atuais. O Censo Escolar de 2022 aponta que existem 80.449 estudantes que possuem baixa visão, 7.308 com cegueira e 626 com surdocergueira matriculados no ensino escolar básico. Desse modo, o objetivo deste texto é relatar a experiência desenvolvida com 30 licenciandos do curso de Pedagogia, da Faculdade de Educação da Universidade Federal do Ceará (FACED/UFC), na qual se buscou o desenvolvimento do Pensamento Geométrico (PG) na abordam dos conceitos de arestas, faces e vértices. Para isso, foi realizada uma oficina no dia 26 de março de 2023, com duração de 3h, abordando os Sólidos de Platão e a Realidade Aumentada (RA), utilizando a metodologia Sequência Fedathi (SF), a qual possui o olhar mais específico para a ação pedagógica do professor. Esta metodologia engloba a concepção do planejamento de ensino (antes), a execução do trabalho docente em sala de aula (durante) e a conclusão da aula (reflexão sobra a prática), com a finalidade de propiciar ao aluno uma postura investigativa e autônoma no decorrer da ação educativa. Os resultados nos revelaram que, apesar de todos os estudantes terem caracterizado a matemática como uma disciplina difícil e muito rigorosa, a oficina possibilitou o contato com novas abordagens metodológicas para uma geometria inclusiva com estudantes com deficiência visual. As considerações finais mostram que houve aprendizado e um bom envolvimento do grupo durante toda a atividade e discussões.

Conservation practices such as no-tillage are necessary to maintain, and/or improve agricultural soil quality. However, straw formation and maintenance in tropical regions is a limiting factor to adopting this system. In this sense, this research aimed to study the effect of cover crops on straw formation, soil quality indicators, and cowpea yield under no-tillage in the Amazon savanna. Two experiments were carried out in consecutive years, on a Latossolo Amarelo. The treatments consisted of the straw of cover crops in a single (S) and intercrop (I) system, as follows: Urochloa brizantha (UBS), Pennisetum glaucum (PGS), Crotalaria juncea (CJS), Canavalia ensiformis (CES), Mucuna aterrima (MAS), U. brizantha and C. juncea (UBCJI), U. brizantha and C. ensiformis (UBCEI), P. glaucum and C. juncea (PGCJI), P. glaucum and C. ensiformis (PGCEI), and spontaneous vegetation (SV). Cowpea was cultivated after desiccation of the cover crops. Most of the straw favored soil particle aggregation, except for C. juncea and SV, which negatively influenced this variable. Basal soil respiration, organic carbon content, potassium, base saturation, geometric mean diameter, macroaggregates, and macroporosity at layer of 0-0.10 m were the most sensitive indicators for improving soil quality in a savanna environment. UBS, UBCJI, and UBCEI were superior to the other treatments in improving soil quality. Almost all treatments increased cowpea yield by up to 29%, except for CJS and SV.

The beneficial effect of growth stimulation caused by low doses of a toxic product such as an herbicide, is due to the phenomenon known as hormesis. Plants affected by this phenomenon show changes in their metabolism and, as a result, are able to increase nutrient absorption and biomass accumulation. Various herbicides have been developed as growth regulators, proving the hormesis hypothesis. The most notable example is the product 2,4-D, originally developed as auxin. This phenomenon can bring benefits for crops, such as increased yield. However, for the technique to be applied in practice, information is still needed to support its potential application in agriculture. The aim of this review was to define the state of the art and measure the effect of the hormesis phenomenon of herbicides in agriculture. To carry out a bibliographical review using the following databases: SciELO, Scopus, Web of Science, Redalyc, EBSCO, and DOAJ. The quality of the studies was assessed using eligibility, inclusion and exclusion criteria, employing the following indexing terms: dichlorophenoxyacetic acid, herbicide subdose, herbicide toxicity, and growth regulator. Based on a summary of the studies identified via electronic or manual searches relating to the phenomenon of hormesis, it was found that the literature on the subject remains ambiguous, with no consensus regarding the real effects of the phenomenon on crop performance. This article further argues that the magnitude of the hormetic effect on crops depends on several factors, such as the subdose, the age of the plant, and other conditions.

Parasitoid wasps are fundamental insects for the biological control of agricultural pests. Despite the importance of wasps as natural enemies for more sustainable and healthy agriculture, the factors that could impact their species richness, abundance, and fitness, such as viral diseases, remain almost unexplored. Parasitoid wasps have been studied with regard to the endogenization of viral elements and the transmission of endogenous viral proteins that facilitate parasitism. However, circulating viruses are poorly characterized. Here, RNA viromes of six parasitoid wasp species are studied using public libraries of next-generation sequencing through an integrative bioinformatics pipeline. Our analyses led to the identification of 18 viruses classified into 10 families (Iflaviridae, Endornaviridae, Mitoviridae, Partitiviridae, Virgaviridae, Rhabdoviridae, Chuviridae, Orthomyxoviridae, Xinmoviridae, and Narnaviridae) and into the Bunyavirales order. Of these, 16 elements were described for the first time. We also found a known virus previously identified on a wasp prey which suggests viral transmission between the insects. Altogether, our results highlight the importance of virus surveillance in wasps as its service disruption can affect ecology, agriculture and pest management, impacting the economy and threatening human food security.

ABSTRACT Studies related to climate change and agricultural value chains have in common the growing concern on conserving water resources. Thus, the concept of the water footprint stands out, which measures the amount of water (in volume) necessary to produce a unit (in mass) of a given product. Among Brazilian agricultural activities, coffee farming emerges as one of the most important, even though the crop is sensitive to potential climatic changes, especially to the increase in temperature and periods of drought. An alternative to mitigate the effects of climate change is shade management, which is common in agroforestry systems. Therefore, the objective of this study was to evaluate the influence of shade management on the water footprint of coffee activity in the region of “Matas de Minas”. The water footprint was calculated for the field and product processing phase. Despite reducing the evapotranspiration of the coffee plant, shade management provided an increase in the water footprint, since it decreased the crop yield. The water footprint data obtained are expressive, with a calculated value of 13,862 m3 t-1 for full sun management and 16,895 m3 t-1 for shade management, in which both are the most recommended for the agricultural sector.

Phlebotomine sand flies are of global significance as important vectors of human disease, transmitting bacterial, viral, and protozoan pathogens, including the kinetoplastid parasites of the genus Leishmania, the causative agents of devastating diseases collectively termed leishmaniasis. More than 40 pathogenic Leishmania species are transmitted to humans by approximately 35 sand fly species in 98 countries with hundreds of millions of people at risk around the world. No approved efficacious vaccine exists for leishmaniasis and available therapeutic drugs are either toxic and/or expensive, or the parasites are becoming resistant to the more recently developed drugs. Therefore, sand fly and/or reservoir control are currently the most effective strategies to break transmission. To better understand the biology of sand flies, including the mechanisms involved in their vectorial capacity, insecticide resistance, and population structures we sequenced the genomes of two geographically widespread and important sand fly vector species: Phlebotomus papatasi, a vector of Leishmania parasites that cause cutaneous leishmaniasis, (distributed in Europe, the Middle East and North Africa) and Lutzomyia longipalpis, a vector of Leishmania parasites that cause visceral leishmaniasis (distributed across Central and South America). We categorized and curated genes involved in processes important to their roles as disease vectors, including chemosensation, blood feeding, circadian rhythm, immunity, and detoxification, as well as mobile genetic elements. We also defined gene orthology and observed micro-synteny among the genomes. Finally, we present the genetic diversity and population structure of these species in their respective geographical areas. These genomes will be a foundation on which to base future efforts to prevent vector-borne transmission of Leishmania parasites.

La pandemia generada por el SARS-CoV2 tuvo serias consecuencias para la población en general y para las comunidades educativas en particular, ya que el confinamiento que se realizó para disminuir los contagios provocó problemas socioemocionales tanto en alumnos como docentes. La ansiedad, el estrés y los sentimientos de soledad y depresión fueron comunes a la mayor parte de los involucrados en el proceso educativo. Asimismo, la falta de socialización también afectó el desarrollo emocional de docentes y estudiantes. El Centro Universitario UAEM Amecameca realizó una serie de acciones para atemperar los problemas socioemocionales de estudiantes y docentes, entre ellos el diálogo, apoyo emocional y la implementación de talleres artísticos y de actividades de cultura física para fomentar la integración de los estudiantes y generar empatía, solidaridad y fortalecer los vínculos entre la comunidad escolar.

IntroductionTheobroma cacao, the cocoa tree, is a target for pathogens, such as fungi from the genera Phytophthora, Moniliophthora, Colletotrichum, Ceratocystis, among others. Some cacao pathogens are restricted to specific regions of the world, such as the Cacao swollen shoot virus (CSSV) in West African countries, while others are expanding geographically, such as Moniliophthora roreri in the Americas. M. roreri is one of the most threatening cacao pathogens since it directly attacks the cacao pods driving a significant reduction in production, and therefore economic losses. Despite its importance, the knowledge about the microenvironment of this pathogen and the cocoa pods is still poorly characterized.MethodsHerein we performed RNA sequencing of spores in differential stages of culture in a medium supplemented with cacao pod extract and mycelium collected of the susceptible variety ICT 7121 naturally infected by the pathogen to evaluate the diversity and transcriptional activity of microorganisms associated with the in vitro sporulation of M. roreri.ResultsOur data revealed a great variety of fungi and bacteria associated with M. roreri, with an exceptional diversity of individuals from the genus Trichoderma sp. Interestingly, the dynamics of microorganisms from different kingdoms varied proportionally, suggesting they are somehow affected by M. roreri culture time. We also identified three sequences similar to viral genomes from the Narnaviridae family, posteriorly confirmed by phylogenetic analysis as members of the genus Narnavirus. Screening of M. roreri public datasets indicated the virus sequences circulating in samples from Ecuador, suggesting a wide spread of these elements. Of note, we did not identify traces of the viral sequences in the M. roreri genome or DNA sequencing, restricting the possibility of these sequences representing endogenized elements.DiscussionTo the best of our knowledge, this is the first report of viruses infecting the fungus of the genus Moniliophthora and only the third description of viruses that are able to parasite elements from the Marasmiaceae family.

Theobroma cacao plantations are of significant economic importance worldwide, primarily for chocolate production. During the harvest and processing of cocoa beans, they are subjected to fermentation either by microorganisms present in the environment (spontaneous fermentation) or the addition of starter cultures, with different strains directly contributing distinct flavor and color characteristics to the beans. In addition to fungi and bacteria, viruses are ubiquitous and can affect the quality of the fermentation process by infecting fermenting organisms, destabilizing microbial diversity, and consequently affecting fermentation quality. Therefore, in this study, we explored publicly available metatranscriptomic libraries of cocoa bean fermentation in Limon Province, Costa Rica, looking for viruses associated with fermenting microorganisms. Libraries were derived from the same sample at different time points: 7, 20, and 68 h of fermentation, corresponding to yeast- and lactic acid bacteria-driven phases. Using a comprehensive pipeline, we identified 68 viral sequences that could be assigned to 62 new viral species and 6 known viruses distributed among at least nine families, with particular abundance of elements from the Lenarviricota phylum. Interestingly, 44 of these sequences were specifically associated with ssRNA phages (Fiersviridae) and mostly fungi-infecting viral families (Botourmiaviridae, Narnaviridae, and Mitoviridae). Of note, viruses from those families show a complex evolutionary relationship, transitioning from infecting bacteria to infecting fungi. We also identified 10 and 3 viruses classified within the Totiviridae and Nodaviridae families, respectively. The quantification of the virus-derived RNAs shows a general pattern of decline, similar to the dynamic profile of some microorganism genera during the fermentation process. Unexpectedly, we identified narnavirus-related elements that showed similarity to segmented viral species. By exploring the molecular characteristics of these viral sequences and applying Hidden Markov Models, we were capable of associating these additional segments with a specific taxon. In summary, our study elucidates the complex virome associated with the microbial consortia engaged in cocoa bean fermentation that could contribute to organism/strain selection, altering metabolite production and, consequently, affecting the sensory characteristics of cocoa beans. [ABSTRACT FROM AUTHOR]

This study reports the virome investigation of pollinator species and other floral visitors associated with plants from the south of Bahia: Aphis aurantii, Atrichopogon sp., Dasyhelea sp., Forcipomyia taiwana, and Trigona ventralis hoozana. Studying viruses in insects associated with economically important crops is vital to understand transmission dynamics and manage viral diseases that pose as threats for global food security. Using literature mining and public RNA next-generation sequencing data deposited in the NCBI SRA database, we identified potential vectors associated with Malvaceae plant species and characterized the microbial communities resident in these insects. Bacteria and Eukarya dominated the metagenomic analyses of all taxon groups. We also found sequences showing similarity to elements from several viral families, including Bunyavirales, Chuviridae, Iflaviridae, Narnaviridae, Orthomyxoviridae, Rhabdoviridae, Totiviridae, and Xinmoviridae. Phylogenetic analyses indicated the existence of at least 16 new viruses distributed among A. aurantii (3), Atrichopogon sp. (4), Dasyhelea sp. (3), and F. taiwana (6). No novel viruses were found for T. ventralis hoozana. For F. taiwana, the available libraries also allowed us to suggest possible vertical transmission, while for A. aurantii we followed the infection profile along the insect development. Our results highlight the importance of studying the virome of insect species associated with crop pollination, as they may play a crucial role in the transmission of viruses to economically important plants, such as those of the genus Theobroma, or they will reduce the pollination process. This information may be valuable in developing strategies to mitigate the spread of viruses and protect the global industry.

Brazilian wheat production is concentrated in the South region and does not fully meet the domestic demand for wheat. The Brazilian Cerrado has become an area of great potential for wheat cultivation due to investments in genetic improvement research. Thus, this study aimed to assess the growth and grain production of wheat cultivars in savannah soil in Roraima, Brazil. The experimental design consisted of randomized blocks with four treatments and five replications. The treatments consisted of the following wheat cultivars: TBIO Aton, TBIO Convicto, TBIO Duque, and TBIO Trunfo. The experimental unit was represented by an area of 18 m2 composed of 14 rows of 6 m, spaced at 20 cm, with approximately 100 plants per meter. The following variables were assessed: plant height, number of spikelets per spike, number of grains per spike, number of grains per spikelet, and grain yield. In general, the four cultivars showed good adaptation to the edaphoclimatic conditions of the Roraima savannah. The cultivars TBIO Convicto and TBIO Aton are superior to the others in terms of grain production. The industrial quality of the cultivars was favorable to the use of grains for the flour industry

No presente ensaio realizamos um exercício de comparação entre as traduções de Romanos 1.1-7 em dez edições da Bíblia em língua portuguesa, a saber: Novo Testamento Interlinear, A Bíblia de Jerusalém, Tradução Ecumênica da Bíblia, Nova Versão Internacional, Bíblia do Peregrino, Bíblia Sagrada Ave Maria, Almeida Revista e Atualizada, Almeida Revista e Corrigida, Nova Tradução Linguagem de Hoje, Tradução de Frederico Lourenço, Nova Bíblia Pastoral. Nossa análise crítica ressalta a ideología e a intencionalidade nas perspectivas das opções de tradução feitas nas diversas Bíblias mencionadas.

This study presents an analysis of the possible use of a viscose rayon (CV) fiber from textile industry wastes to develop a reinforced cementitious composite as an alternative for textile discharge valorization. Several techniques were used to characterize precursor fibrous waste material such as SEM, FT-IR, DSC, and TGA. The experimental studies were conducted based on a conventional cementitious mortar (control) and four different fiber contents (0.5, 1, 2, and 4 wt%). For mechanical behavior analysis, uniaxial compressive strength tests were carried out at different ages (7, 14, and 28 days after production). The results showed favorable CV fiber addition as reinforcement up to a maximum limit. The optimum concentration of fiber was 0.5 wt% (FRC0.5), which provided 28 days of higher compression strength. The addition of CV waste as reinforcement in cementitious matrix resulted in an improved compressive strength above 20.6% compared to the conventional non-reinforced mortar. Furthermore, CV fiber addition improved the ductile behavior of the new composite allowing a controlled failure, even after maximum rupture loading.

Agroforestry systems (AFS) for cocoa production combine traditional land-use practices with local biodiversity conservation, resulting in both ecological and agricultural benefits. The cacao-cabruca AFS model is widely implemented in regions of the Brazilian Atlantic Forest. Carpotroche brasiliensis (Raddi) A. Gray (Achariaceae) is a tree found in cabruca landscapes that is often used for reforestation and biotechnological applications. Despite its importance, we still lack information about viruses circulating in C. brasiliensis, particularly considering the possibility of spillover that could affect cocoa production. In our study, we analyzed the Carpotroche brasiliensis virome from Atlantic Forest and cacao-cabruca AFS regions using metatranscriptomics from several vegetative and reproductive organs. Our results revealed a diverse virome detecting near-complete or partial coding sequences of single- and double-stranded DNA and RNA viruses classified into at least six families (Botourmiaviridae, Bromoviridae, Caulimoviridae, Genomoviridae, Mitoviridae, and Rhabdoviridae) plus unclassified elements. We described with high confidence the near-complete and the partial genomes of two tentative novel viruses: Carpotroche-associated ilarvirus and Carpotroche-associated genomovirus, respectively. Interestingly, we also described sequences likely derived from a rhabdovirus, which could represent a novel member of the genus Gammanucleorhabdovirus. We observed higher viral diversity in cacao-cabruca AFS and reproductive organs of C. brasiliensis with preferential tropism to fruits, which could directly affect production. Altogether, our results provide data to better understand the virome in this unexplored agroecological interface, such as cacao-cabruca AFS and forest ecosystem, providing information on the aspects of virus–plant interactions.

The fungus Moniliophthora perniciosa secretes protein effectors that manipulate the physiology of the host plant, but few effectors of this fungus have had their functions confirmed. We performed functional characterization of a promising candidate effector of M. perniciosa. The inoculation of rBASIDIN at 4 µmol L−1 in the mesophyll of leaflets of Solanum lycopersicum caused symptoms of shriveling within 6 h without the presence of necrosis. However, when sprayed on the plant at a concentration of 11 µmol L−1, it caused wilting symptoms only 2 h after application, followed by necrosis and cell death at 48 h. rBASIDIN applied to Theobroma cacao leaves at the same concentration caused milder symptoms. rBASIDIN caused hydrogen peroxide production in leaf tissue, damaging the leaf membrane and negatively affecting the photosynthetic rate of Solanum lycopersicum plants. Phylogenetic analysis indicated that BASIDIN has orthologs in other phytopathogenic basidiomycetes. Analysis of the transcripts revealed that BASIDIN and its orthologs are expressed in different fungal species, suggesting that this protein is differentially regulated in these basidiomycetes. Therefore, the results of applying BASIDIN allow the inference that it is an effector of the fungus M. perniciosa, with a strong potential to interfere in the defense system of the host plant.

Soil use and management systems modify phosphorus (P) dynamics by changing the different P compartments. However, the interaction between land-use change and altitude is not clear. This study aimed to evaluate the effect of land use on P forms in different soil aggregate classes of Ultisols located at two altitudes. Soil samples were collected in four land-use systems (agroforestry system, secondary forest, pasture, and eucalyptus) located at two altitudes in the Atlantic Forest biome, Brazil. Phosphorus fractions were assessed using sequential fractionation. In general, recalcitrant forms represented about 70% in the agroforestry system, 66% in the eucalyptus, 65% in the pasture, and 57% in the secondary forest relative to the total P content of all land uses at both altitudes. The soil aggregate classes of smaller diameter presented higher P contents in all fractions. The smallest soil aggregate classes showed up to 65% more P than the classes with smaller diameters in the different land-use systems and altitudes.

Narrow-leafed lupin (Lupinus angustifolius) is an important dryland crop, providing a protein source in global grain markets. While agronomic practices have successfully controlled many dicot weeds among narrow-leafed lupins, the closely related sandplain lupin (Lupinus cosentinii) has proven difficult to control, reducing yield and harvest quality. Here, we successfully trained a segmentation model to detect sandplain lupins and differentiate them from narrow-leafed lupins under field conditions. The deep learning model was trained using 9171 images collected from a field site in the Western Australian grain belt. Images were collected using an unoccupied aerial vehicle at heights of 4, 10, and 20 m. The dataset was supplemented with images sourced from the WeedAI database, which were collected at 1.5 m. The resultant model had an average precision of 0.86, intersection over union of 0.60, and F1 score of 0.70 for segmenting the narrow-leafed and sandplain lupins across the multiple datasets. Images collected at a closer range and showing plants at an early developmental stage had significantly higher precision and recall scores (p-value < 0.05), indicating image collection methods and plant developmental stages play a substantial role in the model performance. Nonetheless, the model identified 80.3% of the sandplain lupins on average, with a low variation (±6.13%) in performance across the 5 datasets. The results presented in this study contribute to the development of precision weed management systems within morphologically similar crops, particularly for sandplain lupin detection, supporting future narrow-leafed lupin grain yield and quality.

Significant scientific advances to elucidate the Moniliophthora perniciosa pathosystem have been achieved in recent years, but the molecular biology of this pathogen-host interaction is still a field with many unanswered questions. In order to present insights at the molecular level, we present the first systematic review on the theme. All told, 1118 studies were extracted from public databases. Of these, 109 were eligible for the review, based on the inclusion and exclusion criteria. The results indicated that understanding the transition from the biotrophic-necrotrophic phase of the fungus is crucial for control of the disease. Proteins with strong biotechnological potential or that can be targets for pathosystem intervention were identified, but studies regarding possible applications are still limited. The studies identified revealed important genes in the M. perniciosa-host interaction and efficient molecular markers in the search for genetic variability and sources of resistance, with Theobroma cacao being the most common host. An arsenal of effectors already identified and not explored in the pathosystem were highlighted. This systematic review contributes to the understanding of the pathosystem at the molecular level, offering new insights and proposing different paths for the development of new strategies to control witches’ broom disease.

Viruses that infect fungi are known as mycoviruses and are characterized by the lack of an extracellular phase. In recent years, the advances on nucleic acids sequencing technologies have led to a considerable increase in the number of fungi-infecting viral species described in the literature, with a special interest in assessing potential applications as fungal biocontrol agents. In the present study, we performed a comprehensive review using Scopus, Web of Science, and PubMed databases to mine mycoviruses data to explore their molecular features and their use in biotechnology. Our results showed the existence of 267 mycovirus species, of which 189 are recognized by the International Committee on Taxonomy of Viruses (ICTV). The majority of the mycoviruses identified have a dsRNA genome (38.6%), whereas the Botourmiaviridae (ssRNA+) alone represents 14% of all mycoviruses diversity. Regarding fungal hosts, members from the Sclerotinicaeae appeared as the most common species described to be infected by mycoviruses, with 16 different viral families identified so far. It is noteworthy that such results are directly associated with the high number of studies and strategies used to investigate the presence of viruses in members of the Sclerotinicaeae family. The knowledge about replication strategy and possible impact on fungi biology is available for only a small fraction of the mycoviruses studied, which is the main limitation for considering these elements potential targets for biotechnological applications. Altogether, our investigation allowed us to summarize the general characteristics of mycoviruses and their hosts, the consequences, and the implications of this knowledge on mycovirus–fungi interactions, providing an important source of information for future studies.

Theobroma cacao is one of the main crops of economic importance in the world as the source of raw material for producing chocolate and derivatives. The crop is the main source of income for thousands of small farmers, who produce more than 80% of the world’s cocoa supply. However, the emergence, re-emergence and proliferation of pathogens, such as Ceratocystis spp., the causative agent of Ceratocystis wilt disease and canker disease, have been affecting the sustainability of many crops. Fungal control is laborious, often depending on fungicides that are expensive and/or toxic to humans, prompting researchers to look for new solutions to counteract the proliferation of these pathogens, including the use of biological agents such as mycoviruses. In this study, we investigated the diversity of microorganisms associated with the T. cacao pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata with a focus on the virome using RNA sequencing data available in public databases. We used a comprehensive bioinformatics pipeline containing several steps for viral sequence enrichment and took advantage of an integrated assembly step composed of different assemblers followed by sequence similarity searches using NCBI nonredundant databases. Our strategy was able to identify four putative C. cacaofunesta viruses (hypovirus, sclerotimonavirus, alphapartitivirus and narnavirus) and six C. fimbriata viruses (three alphaendornaviruses, one victorivirus and two mitoviruses). All the viral sequences identified showed similarity to viral genomes in public databases only at the amino acid level, likely representing new viral species. Of note, we present the first report of viruses associated with the cacao pathogens C. cacaofunesta and C. fimbriata and the second report of viral species infecting members of the Ceratocystidaceae family. Our findings highlight the need for further prospective studies to uncover the real diversity of fungus-infecting viruses that can contribute to the development of new management strategies.

Pastures cover, in Brazil, around 165 million ha, most of them are at some degradation level. Recovering these pastures is essential. The objective of this study was to evaluate the sensibility of soil organic matter (SOM) and its physical attributes in pasture soil at different degradation levels (1 to 4), Capoeiras (1 and 2) and secondary Forest (Control). Samples were collected at four depths (0.00-0.05, 0.05-0.10, 0.10-0.20 and 0.20-0.40 m). We verified higher values for SOM and hydraulic conductivity (Ko) in Forest, especially in the most superficial layer (0.00-0.05). We noted low values for macroporosity in this area. In all areas under Pastures lower values for Ko were observed in the superficial layer (0.00-0.05 m) when compared to layers 0.05-0.10 and 0.10-0.20 m, which indicates that the pasture management and cattle trampling negatively affect the physical quality of the pastures. For field capacity and wilting point the average values were observed in Pasture 4 (more degraded), which caused higher values for water availability. According to the principal components analysis, except for SOM at 0.20-0.40 m, all variables studied presented correlation above 0.6. From grouping analysis, we separated 3 groups: Forest; Capoeiras 1 and Capoeira 2 and Pastures 1 to 4.

Abstract The microstructures of steels are altered when exposed to high working temperatures for long periods, which results in varied physical and chemical proprieties. This study evaluates the erosive wear behavior of ASTM A106 steel after it is exposed to high temperatures for long durations. Samples of normalized ASTM A106GrB steel were placed in a muffle furnace at 530 and 630 °C for 50, 100, and 200 h. The erosive wear test was conducted at a speed of 20 m/s for 30 min at 450 °C with an incidence angle of 30°. Irregular alumina was used as the erodent material. The surface characterization was performed using scanning electron microscopy to determine the distribution homogeneity, morphological conditions, quantity, and spheroidization degree of the carbides. The erosive wear test results indicated that there was no volume loss after 50 h of heat treatment at 530 °C. The maximum spheroidization degree was 0.71 after 200 h at 630 °C, which decreased the hardness from 220 to 140 HV. Additionally, the volume loss reached 2.52 cm3. Therefore, the use of the ASTM A106GrB steel at working temperatures higher than 530 °C for more than 50 h decreased the mechanical behavior and the wear resistance, thereby decreasing the lifetime of the system.