36 results on '"Rocco Perniola"'
Search Results
2. Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)
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Rosa Anna Milella, Mirko De Rosso, Marica Gasparro, Isabella Gigante, Giambattista Debiase, Lucia Rosaria Forleo, Antonio Domenico Marsico, Rocco Perniola, Valeria Tutino, Maria Notarnicola, Riccardo Velasco, and Riccardo Flamini
- Subjects
breeding ,phenolic compounds ,seedless grape ,antioxidant ,anticancer ,quality improvement ,Plant culture ,SB1-1110 - Abstract
Grapes represent a significant source of phenolic compounds known for their health-promoting properties, such as antioxidant capacity on normal cells and prooxidant activity on tumor cells. The genotype highly affects the polyphenolic composition in grapes and, consequently, the nutritional quality of berries. This work aimed to characterize the phenolic composition, the antioxidant, and anticancer activity of grape skin extracts (GSEs) of nine new table grape genotypes selected from a breeding program to obtain new cultivars of seedless table grapes, well adapted to the climatic change and with higher nutraceutical properties. The grape polyphenolic profile was characterized by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry analysis. GSE antioxidant activity was determined by the ABTS, DPPH, and ORAC assays; GSE cell growth inhibition test was carried out in the Caco2 human cancer cell line. The nine GSEs showed different flavonoid and non-flavonoid profiles, and all possessed antioxidant activity, with the ‘Aika N.’, ‘Turese N.’, and ‘Egnatia N.’ the most active. As anticancer activity against the tested cancer cell line, ‘Daunia N.’ and ‘Apenestae N.’ showed the EC50 after 24 h of 35.60 µg/mL and 150.91 µg/mL, respectively. The relationship between polyphenolic profile and the antioxidant and anticancer activity of GSE was also investigated. Interestingly, among the different classes of polyphenolics, flavan-3-ols e proanthocyanidins showed the highest positive correlation with the anticancer activity of extracts. These findings can be helpful for the preparation of new extracts for the pharmaceutical and nutraceutical industry and geneticists working in vine breeding programs.
- Published
- 2023
- Full Text
- View/download PDF
3. Use of Artificial Neural Networks and NIR Spectroscopy for Non-Destructive Grape Texture Prediction
- Author
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Teodora Basile, Antonio Domenico Marsico, and Rocco Perniola
- Subjects
PCA ,ANN ,PLS ,MC-UVE ,β coefficients ,R statistics ,Chemical technology ,TP1-1185 - Abstract
In this article, a combination of non-destructive NIR spectroscopy and machine learning techniques was applied to predict the texture parameters and the total soluble solids content (TSS) in intact berries. The multivariate models obtained by building artificial neural networks (ANNs) and applying partial least squares (PLS) regressions showed a better prediction ability after the elimination of uninformative spectral ranges. A very good prediction was obtained for TSS and springiness (R2 0.82 and 0.72). Qualitative models were obtained for hardness and chewiness (R2 0.50 and 0.53). No satisfactory calibration model could be established between the NIR spectra and cohesiveness. Textural parameters of grape are strictly related to the berry size. Before any grape textural measurement, a time-consuming berry-sorting step is compulsory. This is the first time a complete textural analysis of intact grape berries has been performed by NIR spectroscopy without any a priori knowledge of the berry density class.
- Published
- 2022
- Full Text
- View/download PDF
4. Color Stabilization of Apulian Red Wines through the Sequential Inoculation of Starmerella bacillaris and Saccharomyces cerevisiae
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Matteo Velenosi, Pasquale Crupi, Rocco Perniola, Antonio Domenico Marsico, Antonella Salerno, Hervè Alexandre, Nicoletta Archidiacono, Mario Ventura, and Maria Francesca Cardone
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HPLC-UV-ESI-MSn ,free anthocyanins ,co-pigmented anthocyanins ,mixed fermentation ,starmerella bacillaris ,PCA ,Organic chemistry ,QD241-441 - Abstract
Mixed fermentation using Starmerella bacillaris and Saccharomyces cerevisiae has gained attention in recent years due to their ability to modulate the qualitative parameters of enological interest, such as the color intensity and stability of wine. In this study, three of the most important red Apulian varieties were fermented through two pure inoculations of Saccharomyces cerevisiae strains or the sequential inoculation of Saccharomyces cerevisiae after 48 h from Starmerella bacillaris. The evolution of anthocyanin profiles and chromatic characteristics were determined in the produced wines at draining off and after 18 months of bottle aging in order to assess the impact of the different fermentation protocols on the potential color stabilization and shelf-life. The chemical composition analysis showed titratable acidity and ethanol content exhibiting marked differences among wines after fermentation and aging. The 48 h inoculation delay produced wines with higher values of color intensity and color stability. This was ascribed to the increased presence of compounds, such as stable A-type vitisins and reddish/violet ethylidene-bridge flavonol-anthocyanin adducts, in the mixed fermentation. Our results proved that the sequential fermentation of Starmerella bacillaris and Saccharomyces cerevisiae could enhance the chromatic profile as well as the stability of the red wines, thus improving their organoleptic quality.
- Published
- 2021
- Full Text
- View/download PDF
5. Native Vineyard Non-Saccharomyces Yeasts Used for Biological Control of Botrytis cinerea in Stored Table Grape
- Author
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Antonio Domenico Marsico, Matteo Velenosi, Rocco Perniola, Carlo Bergamini, Scott Sinonin, Vanessa David-Vaizant, Flavia Angela Maria Maggiolini, Alexandre Hervè, Maria Francesca Cardone, and Mario Ventura
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biological control ,table grape ,Botrytis cinerea ,yeasts ,Lachancea thermotolerans ,Metschnikowia pulcherrima ,Biology (General) ,QH301-705.5 - Abstract
Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses of fresh fruit quality and vegetables during storage, distribution, and consumption. The current control strategy is the use of SO2 generator pads whose application is now largely under observation. A high quantity of SO2 can be deleterious for fresh fruits and vegetables and it is not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological approaches devoid of chemicals. In this direction, we carried out our study in isolating five different non-Saccharomyces yeast strains from local vineyards in the South of Italy as possible BCA. We performed both in vitro and in vivo assays in semi-commercial conditions on detached grape berries stored at 0 °C, simulating the temperature normally used during cold storage, and obtained relevant results. We isolated three M. pulcherrima strains and one L. thermotolerans strain able to largely antagonize the development of the B. cinerea, at both in vitro and in vivo conditions. In particular, we detected the ability of the three isolates of M. pulcherrima strains Ale4, N20/006, and Pr7 and the L. thermotolerans strain N10 to completely inhibit (100% in reduction) the mycelial growth of B. cinerea by producing fungistatic compounds. We found, using an extracellular lytic enzymes activity assay, that such activity could be related to lipid hydrolyzation, β-1,3-glucanase and pectinase activity, and pectinase and protease activity, depending on the yeasts used. Results from our in vitro assays allowed us to hypothesize for M. pulcherrima strains Ale4 and N20/006 a possible combination of both the production of soluble metabolites and volatile organic compounds to antagonize against B. cinerea growth. Moreover, in semi-commercial conditions, the M. pulcherrima strain N20/006 and L. thermotolerans strain N10 showed relevant antagonistic effect also at low concentrations (with a significantly reduction of ‘slip skin’ incidence of 86.4% and 72.7%, respectively), thus highlighting a peculiar property to use in commercial development for organic agriculture and the handling process.
- Published
- 2021
- Full Text
- View/download PDF
6. NIR Analysis of Intact Grape Berries: Chemical and Physical Properties Prediction Using Multivariate Analysis
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Teodora Basile, Antonio Domenico Marsico, and Rocco Perniola
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NIR ,grape ,hardness ,crunchiness ,TSS ,iPLS ,Chemical technology ,TP1-1185 - Abstract
Texture characteristics are valuable parameters in the perceived quality and overall acceptability of fresh fruit. The characterization of grape texture attributes, such as firmness and crunchiness, is usually performed by sensory analysis or instrumental texture analysis. Both methodologies are destructive. Hence, it is not possible to test multiple times or perform any other analysis on the same sample. In this article, near-infrared (NIR) spectroscopy was applied to intact berries of table grape cv. Regal Seedless. NIR spectra were employed to predict both the physical parameter “hardness”, which is correlated with the crunchiness of berry flesh and the sweetness, which is correlated with the total soluble solids content (TSS, as °Brix). The chemometric analysis was carried out exclusively based on an open-source software environment, producing results readily usable for any operator, besides the specific level of experience with NIR spectroscopy.
- Published
- 2021
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7. Unraveling the Deep Genetic Architecture for Seedlessness in Grapevine and the Development and Validation of a New Set of Markers for VviAGL11-Based Gene-Assisted Selection
- Author
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Nallatt Ocarez, Nicolás Jiménez, Reynaldo Núñez, Rocco Perniola, Antonio Domenico Marsico, Maria Francesca Cardone, Carlo Bergamini, and Nilo Mejía
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seedlessness ,stenospermocarpy ,qtls ,snps ,ssrs ,gene-assisted selection ,vviagl11 ,Genetics ,QH426-470 - Abstract
Seedless inheritance has been considered a quasi-monogenic trait based on the VvAGL11 gene. An intragenic simple sequence repeat (SSR) marker, p3_VvAGL11, is currently used to opportunely discard seeded progeny, which represents up to 50% of seedlings to be established in the field. However, the rate of false positives remains significant, and this lack of accuracy might be due to a more complex genetic architecture, some intrinsic flaws of p3_VvAGL11, or potential recombination events between p3_VvAGL11 and the causal SNP located in the coding region. The purpose of this study was to update the genetic architecture of this trait in order to better understand its implications in breeding strategies. A total of 573 F1 individuals that segregate for seedlessness were genotyped with a 20K SNP chip and characterized phenotypically during four seasons for a fine QTL mapping analysis. Based on the molecular diversity of p3_VvAGL11 alleles, we redesigned this marker, and based on the causal SNP, we developed a qPCR-HRM marker for high-throughput and a Tetra-ARMS-PCR for simple predictive analyses. Up to 10 new QTLs were identified that describe the complex nature of seedlessness, corresponding to small but stable effects. The positive predictive value, based on VvAGL11 alone (0.647), was improved up to 0.814 when adding three small-effect QTLs in a multi-QTL additive model as a proof of concept. The new SSR, 5U_VviAGL11, is more informative and robust, and easier to analyze. However, we demonstrated that the association can be lost by intragenic recombination and that the e7_VviAGL11 SNP-based marker is thus more reliable and decreases the occurrence of false positives. This study highlights the bases of prediction failure based solely on a major gene and a reduced set of candidate genes, in addition to opportunities for molecular breeding following further and larger validation studies.
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- 2020
- Full Text
- View/download PDF
8. FT-NIR Analysis of Intact Table Grape Berries to Understand Consumer Preference Driving Factors
- Author
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Teodora Basile, Antonio Domenico Marsico, Maria Francesca Cardone, Donato Antonacci, and Rocco Perniola
- Subjects
nir ,pls ,pca ,correlogram ,sensory analysis ,Chemical technology ,TP1-1185 - Abstract
Fourier-transform near infrared spectroscopy (FT-NIR) is a technique used in the compositional and sensory analysis of foodstuffs. In this work, we have measured the main maturity parameters for grape (sugars and acids) using hundreds of intact berry samples to build models for the prediction of these parameters from berries of two very different varieties: “Victoria” and “Autumn Royal”. Together with the chemical composition in terms of sugar and acidic content, we have carried out a sensory analysis on single berries. Employing the models built for sugars and acids it was possible to learn the sweetness and acidity of each berry before the destructive sensory analysis. The direct correlation of sensory data with FT-NIR spectra is difficult; therefore, spectral data were exported from the spectrometer built-in software and analyzed with R software using a statistical analysis technique (Spearman correlation) which allowed the correlation of berry appreciation data with specific wavelengths that were then related to sugar and acidic content. In this article, we show how it is possible to carry out the analysis of single berries to obtain data on chemical composition parameters and consumer appreciation with a fast, simple, and non-destructive technique with a clear advantage for producers and consumers.
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- 2020
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9. Utilizzo di vitigni di antica coltivazione del Meridione d'Italia per la produzione di vini passiti di pregio
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Antonacci Donato, Velenosi Matteo, Rocco Perniola, Crupi Pasquale, Ventura Mario, and Bergamini Carlo
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Microbiology ,QR1-502 ,Physiology ,QP1-981 ,Zoology ,QL1-991 - Abstract
In recent years the wine sector has been characterized by an increased focus on the diversification of wine products. This change has been welcomed by consumers. In fact, today's consumers are willing to experiment with new products and unusual combinationswhich has stimulated research aimed at the discovery of native grape varieties suitable for for winemaking. In this context, particular attention are receiving vines for the production of dessert wines. Many regions have made efforts in the search for varieties and techniques able to better promote the drying process. At CREA-VE Turi-BA, there is an ongoing recovery and exploitation of indigenous grape varieties in the regions of Southern Italy and today more than 3,000 accessions of wine grapes are preserved. In the present work we have investigate the possibility of the use of certain accessions of Vitis vinifera varieties (collection CREA-VE Turi-BA) deemed attractive for the production of dessert wines. Accessions presenting a good sugar content and a good titratable acidity were selected; namely, Greco bianco b. (named Q19) and Mantonico bianco b. (named R8) were compared to the cv of Malvasia di Lipari b. Ampelographic and ampelometric analyses, followed by molecular analysis, were carried out for varietal assessment. A rate of the grapes were fermented immediately after harvest with standard procedure, in comparison to rates vinified after withering. The latter was made in a greenhouse type structure with parts of natural and unforced air, without any additional energy expense. At the end of the process, a loss in weight of the grapes equal to 30–35% was realized. The grapes are then stemmed, crushed, pressed and fermented by selected yeast under controlled temperature. Upon completion of the fermentation and subsequent stabilization, the wines were analyzed for the most important enological parameters and subjected to evaluation by a panel of expert tasters. Chemical analysis of wines showed that an alcohol content ranging to 11–12% volume was reached with in the standard wine-making; whilst, for the vinification of dried grapes the alcohol content was equal to 16% vol for Mantonico bianco b. and 18% vol. for and Greco bianco. Organoleptic analysis of the wines showed a greater appreciation for both of dessert wines. The best ratings were given for the olfactory characteristics (floral and fruity aromas) and taste characteristics. In particular, the Greco bianco b. had excellent scores slightly lower than Malvasia di Lipari b. Therefore, the tested varieties have shown a good potential for the production of dessert wines related to typicality of the grape and the region. Dessert wines produced, have a complex flavor profile and interesting for the high acidic component, which improved the taste perception of wine and attenuated the sensation of sweetness. That feature could allow a good wine aging, too.
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- 2017
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10. Production of ready to drink red and rosé wines from new seedless grapevine crossbreeds
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Antonacci Donato, Velenosi Matteo, Rocco Perniola, Basile Teodora, Forleo Lucia Rosaria, Marsico Antonio Domenico, Bergamini Carlo, and Cardone Maria Francesca
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Microbiology ,QR1-502 ,Physiology ,QP1-981 ,Zoology ,QL1-991 - Abstract
Monomeric and polymeric flavan-3-ols (proanthocyanidins) content in grapes is higher in seeds compared to berry skins. Monomeric flavan-3-ols are more astringent, however, they can combine with other monomer, with anthocyanins and with mannoproteins released by yeast and therefore lose their harsh features in wines. Proanthocyanidins extracted during fermentation and maceration processes in red wines, are important for the organoleptic characteristics of the product and for its aging. There is a difference between skins and seeds proanthocyanidins, with the latter being perceived as more harsh and astringent. One of the most important purposes of refinement and aging of red wines very rich in polyphenols is the slow loss of bitterness. Instead, for wines ready to drink seeds tannins can give bitter overtones, therefore reducing their quality since consumers generally prefer a reduced astringency and attenuated bitterness. This paper investigates the possibility of employ some new seedless grapes crossings of Vitis vinifera L., obtained in recent breeding programs carried out at the CREA-VE of Turi, for the production of improved red and rosé wines made with traditionally red winemaking.
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- 2017
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11. Uva da tavola ipocalorica, possibilità di coltivazione e nuove prospettive di mercato
- Author
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Donato Antonacci, Vittorio Alba, Rocco Perniola, Sabino Roccotelli, and Rosalinda Genghi
- Subjects
Microbiology ,QR1-502 ,Physiology ,QP1-981 ,Zoology ,QL1-991 - Abstract
According to the CODEX STAN 255 (2007), table grapes can be harvested when the refractometric index reaches at least 16° Brix. Grapes with a lower refractometric index are accepted provided the sugar/acid ratio (Total Soluble Solids/Tritatable Acidity – TSS/TA) is at least equal to 20:1 if the Brix level is comprised between 12.5° and 14° Brix, 18:1 if the Brix level is comprised between 14° and 16° Brix. Some table grapes grown in Mediterranean climate can accumulate TSS higher than 16° Brix, with low levels of acidity, thus leading to a TSS/AT ratio even greater than 30: 1. Grapes that accumulate TSS less than 12.5° Brix and low TA levels, hardly reach the recommended ratio TSS/TA of 20:1, rather stopping at lower values. Based on these considerations in 2012 an experimental trial of four years started on a total of 103 table grape cvs. Phenological phases in relation to the acidic and sugar content were measured in order to assess the TSS/TA ratio and build regression lines with TSS. Almost all cvs. showed a significant linear regression, with R2 values higher than 0.80 and a standard error between 00.18 and 1.74. TSS values of 103 cvs. at TSS/TA ratio of 20:1, predicted by means of linear regression equations, , were subjected to cluster analysis based on Euclidean Distance. We identified nine clusters, of which the first two with the predicted TSS between 10.04° and 11.77° Brix (7 cvs.) and 12.08° and 12.62° Brix (7 cvs.), respectively. Some of the cvs. showing TSS values below 12.5° Brix at the TSS/TA of 20: 1, were subjected to a test panel of 24 tasters, who were asked to indicate the acceptability of these grapes on hedonic scale of 9 points. Preliminary results showed that for some table grapes with TSS below 12.5° Brix, a SST/AT ratio lower than the recommended 20:1 can be proposed, while preserving an acceptability by the consumer. These results suggest a more modern perspective on grapes nutritional intake, which takes into account the real needs of Western countries where diabetes, hyperglycemia, obesity and cardiovascular diseases are the main dismetabolisms arising from a diet too rich in calories. These grapes thus represent a new frontier and an opportunity for producers of table grapes, who want to enterprise a market share on “grape diet”, and represent a genetic basis for breeding aimed at obtaining new hypocaloric grape cultivars.
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- 2017
- Full Text
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12. Study of the Influence of Different Yeast Strains on Red Wine Fermentation with NIR Spectroscopy and Principal Component Analysis
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Teodora Basile, Antonio Domenico Marsico, Donato Antonacci, Rocco Perniola, Matteo Velenosi, Vittorio Alba, and Maria Francesca Cardone
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Primitivo ,0301 basic medicine ,Wine ,Fermentation in winemaking ,Chemistry ,030106 microbiology ,Acetaldehyde ,NIR spectroscopy ,food and beverages ,Aleatico nero ,04 agricultural and veterinary sciences ,Ethanol fermentation ,040401 food science ,Sensory analysis ,Yeast ,Saccharomyces cerevisiae ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,alcoholic fermentation ,Starmerella bacillaris ,Fermentation ,Food science ,Negramaro ,Winemaking - Abstract
Alcoholic fermentation is a key step in wine production. Indeed, a wide range of compounds, which strongly affect the sensory properties of wine, is produced during this process. While Saccharomyces cerevisiae yeast cultures are commonly employed in winemaking to carry on the fermentation process, some non-Saccharomyces species have recently gained attention due to their ability to produce various metabolites of oenological interest. The use of different yeasts strains usually results in wines with different sensory properties, despite being obtained from the same grape variety. In this paper, we tested the feasibility of using near-infrared spectroscopy (NIR) to discriminate among red wines from three different grape varieties produced with pure S. cerevisiae or by mixed fermentation with a promising non-Saccharomyces yeast, namely the Starmeriella bacillaris, which usually yields wines with significant amounts of glycerol and low levels of ethanol, acetic acid, and acetaldehyde. A principal component analysis (PCA) performed on the NIR spectra was used to search for differences in the samples. The NIR results have been compared with both basic wine parameters and sensory analysis data.
- Published
- 2018
13. Native Vineyard Non-Saccharomyces Yeasts Used for Biological Control of Botrytis cinerea in Stored Table Grape
- Author
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Mario Ventura, Maria Francesca Cardone, Vanessa David-Vaizant, Scott Sinonin, Rocco Perniola, Antonio Domenico Marsico, Alexandre Hervè, Flavia Angela Maria Maggiolini, Matteo Velenosi, and Carlo Bergamini
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0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,Food spoilage ,Cold storage ,biological control ,yeasts ,Microbiology ,Saccharomyces ,Article ,040501 horticulture ,03 medical and health sciences ,Botrytis cinerea ,Lachancea thermotolerans ,Virology ,Food science ,Pectinase ,lcsh:QH301-705.5 ,biology ,Chemistry ,04 agricultural and veterinary sciences ,biology.organism_classification ,Metschnikowia pulcherrima ,Yeast ,lcsh:Biology (General) ,Postharvest ,0405 other agricultural sciences ,table grape - Abstract
Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses of fresh fruit quality and vegetables during storage, distribution, and consumption. The current control strategy is the use of SO2 generator pads whose application is now largely under observation. A high quantity of SO2 can be deleterious for fresh fruits and vegetables and it is not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological approaches devoid of chemicals. In this direction, we carried out our study in isolating five different non-Saccharomyces yeast strains from local vineyards in the South of Italy as possible BCA. We performed both in vitro and in vivo assays in semi-commercial conditions on detached grape berries stored at 0 °C, simulating the temperature normally used during cold storage, and obtained relevant results. We isolated three M. pulcherrima strains and one L. thermotolerans strain able to largely antagonize the development of the B. cinerea, at both in vitro and in vivo conditions. In particular, we detected the ability of the three isolates of M. pulcherrima strains Ale4, N20/006, and Pr7 and the L. thermotolerans strain N10 to completely inhibit (100% in reduction) the mycelial growth of B. cinerea by producing fungistatic compounds. We found, using an extracellular lytic enzymes activity assay, that such activity could be related to lipid hydrolyzation, b-1,3-glucanase and pectinase activity, and pectinase and protease activity, depending on the yeasts used. Results from our in vitro assays allowed us to hypothesize for M. pulcherrima strains Ale4 and N20/006 a possible combination of both the production of soluble metabolites and volatile organic compounds to antagonize against B. cinerea growth. Moreover, in semi-commercial conditions, the M. pulcherrima strain N20/006 and L. thermotolerans strain N10 showed relevant antagonistic effect also at low concentrations (with a significantly reduction of ‘slip skin’ incidence of 86.4% and 72.7%, respectively), thus highlighting a peculiar property to use in commercial development for organic agriculture and the handling process.
- Published
- 2021
14. Girdling and gibberellic acid effects on yield and quality of a seedless red table grape for saving irrigation water supply
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Donato Antonacci, Rocco Perniola, Michele Savino, Pasquale Crupi, Rosalinda Genghi, and Antonio Coletta
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0106 biological sciences ,0301 basic medicine ,chemistry.chemical_classification ,Irrigation ,Table grape ,Soil Science ,Plant Science ,01 natural sciences ,03 medical and health sciences ,Horticulture ,chemistry.chemical_compound ,030104 developmental biology ,Flavonols ,chemistry ,Agronomy ,Anthocyanin ,Girdling ,Yield (wine) ,Botany ,Viticulture ,Agronomy and Crop Science ,Gibberellic acid ,010606 plant biology & botany ,Mathematics - Abstract
Table grapes need of substantial water supply for achieving commercial requirements. Viticulture practices such as girdling (G) and gibberellic acid (GA) application, as well as water supply, can improve table grape quality. The study, which was conducted in two consecutive seasons (2010–2011), aimed to assess the counterbalance effect of these viticulture practices on yield and quality parameters and flavonoids composition in case of a significant and unusual irrigation water reduction (40%) applied to a table grape variety. The data from the two-way ANOVA and PCA analyses indicated that viticulture practices were clearly related to anthocyanins and flavonols variations whereas water management appeared mainly involved in the yield parameters variability. Specifically, the reduced water supply (RWS) decreased the grape yield (−20%) with respect to full water supply (FWS); by contrast GA, G, and G × GA treatments determined an increment of grape production ranging from 10 to 23%, independently from irrigation strategy. Moreover, G, in particular applied to RWS grapes, was able to improve the total soluble solids over titrable acidity (TSS/TA), a ratio strictly related to the quality perception by the consumer. Total anthocyanins were found positively linked to FWS whereas flavanols content was indifferent to water management. Instead viticulture practices seemed to have a greater impact on anthocyanin composition, considering that in RWS grapes under GA condition, higher contents of malvidin and peonidin (mainly responsible for the color stability of the skins) were revealed. Furtheremore, catechins and rutin appeared significantly enhanced by G and G × GA, and GA, respectively. From gathered findings, it can be concluded that suitable viticulture practices can allow a sensible reduction of water supply during table grape growth cycle without detrimental effects on yield and quality.
- Published
- 2016
15. Grapevine adaptation to drought: New candidate genes for the genotype-dependent response
- Author
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Donato Antonacci, Maria Francesca Cardone, Riccardo Velasco, Pasquale Crupi, Silvia Rotunno, Carlo Bergamini, Mario Ventura, Claudia Rita Catacchio, Fiammetta Alagna, Rocco Perniola, Cardone, M. F., Perniola, R., Catacchio, C. R., Alagna, F., Rotunno, S., Crupi, P., Antonacci, D., Velasco, R., Ventura, M., and Bergamini, C.
- Subjects
0106 biological sciences ,0301 basic medicine ,Candidate gene ,Stomatal conductance ,Environmental Engineering ,Osmotic shock ,lcsh:QR1-502 ,Carbohydrate metabolism ,Biology ,Photosynthesis ,01 natural sciences ,Industrial and Manufacturing Engineering ,lcsh:Microbiology ,lcsh:Physiology ,03 medical and health sciences ,Botany ,lcsh:Zoology ,Cultivar ,lcsh:QL1-991 ,lcsh:QP1-981 ,fungi ,food and beverages ,030104 developmental biology ,Cell wall organization ,Adaptation ,010606 plant biology & botany - Abstract
Grapevine requires irrigation supply for its cultivation, especially in the arid and semiarid geographic areas. As consequence of the severe climatic changes, water consumption is becoming more and more important as environmental and cost factor that needs to be reduced. Water deficiency can affect berry and wine quality depending on the extent of plant perceived stress, which is a cultivar specific trait. In a four-year project, we tested the physiological and molecular responses to water deficiency of two different table grape cultivars, Italia and Autumn Royal, and we highlighted that they differently adapted to drought stress conditions. Physiological analyses on field-growth plants showed cultivar-specific variations in photosynthetic carbon assimilation and, stomatal conductance under water deficiency. We further combined “omic” analyses to identify candidate genes involved in drought stress response and adaptative traits. Microarray analyses revealed a broad response of cultivar Italia to drought stress conditions characterized by the modulation of 1037 genes involved in biological processes as cell wall organization, carbohydrate metabolism, ROS response, response to hormone and osmotic stress. On the contrary, Autumn Royal response was limited to the modulation of only 29 genes mainly involved in plant stress response, nitrogen metabolism and hormone signal transduction. Our data highlighted that ABA-perception and –signalling are key factors mediating the varietal-specific behavior of the early response to drought.
- Published
- 2019
16. Transcriptomic and genomic structural variation analyses on grape cultivars reveal new insights into the genotype-dependent responses to water stress
- Author
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Fiammetta Alagna, Carlo Bergamini, Claudia Rita Catacchio, Mario Ventura, Francesco Maria Calabrese, Pasquale Crupi, Silvia Rotunno, Rocco Perniola, Maria Francesca Cardone, Donato Antonacci, Catacchio, C. R., Alagna, F., Perniola, R., Bergamini, C., Rotunno, S., Calabrese, F. M., Crupi, P., Antonacci, D., Ventura, M., and Cardone, M. F.
- Subjects
0301 basic medicine ,Candidate gene ,Osmotic shock ,lcsh:Medicine ,Biology ,Article ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Plant Growth Regulators ,Cell Wall ,Gene Expression Regulation, Plant ,Stress, Physiological ,Botany ,Vitis ,Cultivar ,lcsh:Science ,Wine ,Multidisciplinary ,Dehydration ,Table grape ,fungi ,lcsh:R ,food and beverages ,Droughts ,030104 developmental biology ,Cell wall organization ,Genomic Structural Variation ,Carbohydrate Metabolism ,lcsh:Q ,Adaptation ,Reactive Oxygen Species ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Grapevine (Vitis vinifera L.) is importantly cultivated worldwide for table grape and wine production. Its cultivation requires irrigation supply, especially in arid and semiarid areas. Water deficiency can affect berry and wine quality mostly depending on the extent of plant perceived stress, which is a cultivar-specific trait. We tested the physiological and molecular responses to water deficiency of two table grape cultivars, Italia and Autumn royal, and we highlighted their different adaptation. Microarray analyses revealed that Autumn royal reacts involving only 29 genes, related to plant stress response and ABA/hormone signal transduction, to modulate the response to water deficit. Instead, cultivar Italia orchestrates a very broad response (we found 1037 differentially expressed genes) that modifies the cell wall organization, carbohydrate metabolism, response to reactive oxygen species, hormones and osmotic stress. For the first time, we integrated transcriptomic data with cultivar-specific genomics and found that ABA-perception and –signalling are key factors mediating the varietal-specific behaviour of the early response to drought. We were thus able to isolate candidate genes for the genotype-dependent response to drought. These insights will allow the identification of reliable plant stress indicators and the definition of sustainable cultivar-specific protocols for water management.
- Published
- 2019
17. Analytical and sensory data correlation to understand consumers' grape preference
- Author
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Maria Francesca Cardone, Teodora Basile, Rocco Perniola, Antonio Domenico Marsico, and Donato Antonacci
- Subjects
0106 biological sciences ,Environmental Engineering ,lcsh:QP1-981 ,Table grape ,Data correlation ,lcsh:QR1-502 ,Sensory system ,04 agricultural and veterinary sciences ,Berry ,Research findings ,040401 food science ,01 natural sciences ,Sensory analysis ,Industrial and Manufacturing Engineering ,Food Analysis ,Preference ,lcsh:Microbiology ,lcsh:Physiology ,0404 agricultural biotechnology ,lcsh:Zoology ,lcsh:QL1-991 ,Biological system ,010606 plant biology & botany - Abstract
NIR spectroscopy is a rapid, economic and not destructive technique employed in food analysis. Concerning fresh table grape, the analysis is usually limited to juices, homogenates or skin extracts which usually give better NIR prediction models. Scanning of intact berries is challenging since each berry has specific features (berry shape, presence of superficial pigmentation, etc.) and, moreover, there are punctual variations even within the same berry. It would be of great interest to obtain information about maturity parameters and consumer's appreciation directly from intact berries, since it would save both time and money. In this article, near infrared (NIR) spectroscopy and chemometric methods have been employed to search for a correlation between sensory analysis and analytical data. The research findings show how it is possible to use a rapid, economic and not destructive emerging technology such as NIR spectroscopy to understand consumer's preference directly from intact berries.
- Published
- 2019
18. Color Stabilization of Apulian Red Wines through the Sequential Inoculation of Starmerella bacillaris and Saccharomyces cerevisiae
- Author
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Mario Ventura, Rocco Perniola, Matteo Velenosi, Maria Francesca Cardone, Hervé Alexandre, Antonio Domenico Marsico, Pasquale Crupi, Antonella Salerno, and Nicoletta Archidiacono
- Subjects
HPLC-UV-ESI-MSn ,Organoleptic ,Saccharomyces cerevisiae ,co-pigmented anthocyanins ,Pharmaceutical Science ,Titratable acid ,Analytical Chemistry ,lcsh:QD241-441 ,03 medical and health sciences ,chemistry.chemical_compound ,Sequential inoculation ,lcsh:Organic chemistry ,free anthocyanins ,Drug Discovery ,Food science ,Physical and Theoretical Chemistry ,030304 developmental biology ,Wine ,PCA ,0303 health sciences ,biology ,mixed fermentation ,030306 microbiology ,Organic Chemistry ,Starmerella bacillaris ,starmerella bacillaris ,food and beverages ,biology.organism_classification ,chemistry ,Chemistry (miscellaneous) ,Anthocyanin ,Molecular Medicine ,Fermentation - Abstract
Mixed fermentation using Starmerella bacillaris and Saccharomyces cerevisiae has gained attention in recent years due to their ability to modulate the qualitative parameters of enological interest, such as the color intensity and stability of wine. In this study, three of the most important red Apulian varieties were fermented through two pure inoculations of Saccharomyces cerevisiae strains or the sequential inoculation of Saccharomyces cerevisiae after 48 h from Starmerella bacillaris. The evolution of anthocyanin profiles and chromatic characteristics were determined in the produced wines at draining off and after 18 months of bottle aging in order to assess the impact of the different fermentation protocols on the potential color stabilization and shelf-life. The chemical composition analysis showed titratable acidity and ethanol content exhibiting marked differences among wines after fermentation and aging. The 48 h inoculation delay produced wines with higher values of color intensity and color stability. This was ascribed to the increased presence of compounds, such as stable A-type vitisins and reddish/violet ethylidene-bridge flavonol-anthocyanin adducts, in the mixed fermentation. Our results proved that the sequential fermentation of Starmerella bacillaris and Saccharomyces cerevisiae could enhance the chromatic profile as well as the stability of the red wines, thus improving their organoleptic quality.
- Published
- 2021
19. Inter-varietal structural variation in grapevine genomes
- Author
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Fabio Anaclerio, Maria Francesca Cardone, Mario Ventura, Carlo Bergamini, Can Alkan, Giorgia Chiatante, Claudia Rita Catacchio, Annamaria Marra, Donato Antonacci, Giuliana Giannuzzi, Rocco Perniola, and Pietro D'Addabbo
- Subjects
DNA copy number variations ,0301 basic medicine ,Candidate gene ,Plant Science ,Procedures ,Polymerase Chain Reaction ,Genome ,Vitis vinifera L ,Table grapes ,Vitis ,Copy-number variation ,In Situ Hybridization, Fluorescence ,Mammals ,Genetics ,Comparative Genomic Hybridization ,High-throughput sequencing ,Vitis vinifera l ,Nucleotides ,Fluorescence in situ hybridization ,food and beverages ,Polymerase chain reaction ,Breweries ,In situ hybridization ,Genome, Plant ,DNA Copy Number Variations ,Crops ,Single-nucleotide polymorphism ,Biology ,Fluorescence ,Chromosomes ,DNA sequencing ,Candidate genes ,Structural variation ,03 medical and health sciences ,Plant genome ,Copy number variations ,Gene ,Genomic variation ,Comparative genomic hybridization ,Copy number variation ,Table grape ,Plant ,Single nucleotide polymorphisms ,Cell Biology ,Throughput ,Single nucleotide polymorphism ,030104 developmental biology ,Genes ,SRP009057 - Abstract
Grapevine (Vitis vinifera L.) is one of the world's most important crop plants, which is of large economic value for fruit and wine production. There is much interest in identifying genomic variations and their functional effects on inter-varietal, phenotypic differences. Using an approach developed for the analysis of human and mammalian genomes, which combines high-throughput sequencing, array comparative genomic hybridization, fluorescent in�situ hybridization and quantitative PCR, we created an inter-varietal atlas of structural variations and single nucleotide variants (SNVs) for the grapevine genome analyzing four economically and genetically relevant table grapevine varieties. We found 4.8 million SNVs and detected 8% of the grapevine genome to be affected by genomic variations. We identified more than 700 copy number variation (CNV) regions and more than 2000 genes subjected to CNV as potential candidates for phenotypic differences between varieties
- Published
- 2016
20. Unraveling the Deep Genetic Architecture for Seedlessness in Grapevine and the Development and Validation of a New Set of Markers for VviAGL11-Based Gene-Assisted Selection
- Author
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Rocco Perniola, Maria Francesca Cardone, Nallatt Ocarez, Nicolás Jiménez, Nilo Mejía, Antonio Domenico Marsico, Carlo Bergamini, and Reynaldo Núñez
- Subjects
0106 biological sciences ,0301 basic medicine ,Candidate gene ,lcsh:QH426-470 ,Genotyping Techniques ,seedlessness ,MADS Domain Proteins ,Single-nucleotide polymorphism ,Computational biology ,Biology ,Quantitative trait locus ,Polymorphism, Single Nucleotide ,01 natural sciences ,Article ,03 medical and health sciences ,Quantitative Trait, Heritable ,gene-assisted selection ,Gene Expression Regulation, Plant ,Genetics ,False positive paradox ,Vitis ,Selection, Genetic ,Genetics (clinical) ,Plant Proteins ,Molecular breeding ,Models, Genetic ,stenospermocarpy ,food and beverages ,Chromosome Mapping ,QTLs ,VviAGL11 ,Major gene ,Genetic architecture ,SNP genotyping ,lcsh:Genetics ,Plant Breeding ,SSRs ,030104 developmental biology ,Seeds ,Microsatellite Repeats ,SNPs ,010606 plant biology & botany - Abstract
Seedless inheritance has been considered a quasi-monogenic trait based on the VvAGL11 gene. An intragenic simple sequence repeat (SSR) marker, p3_VvAGL11, is currently used to opportunely discard seeded progeny, which represents up to 50% of seedlings to be established in the field. However, the rate of false positives remains significant, and this lack of accuracy might be due to a more complex genetic architecture, some intrinsic flaws of p3_VvAGL11, or potential recombination events between p3_VvAGL11 and the causal SNP located in the coding region. The purpose of this study was to update the genetic architecture of this trait in order to better understand its implications in breeding strategies. A total of 573 F1 individuals that segregate for seedlessness were genotyped with a 20K SNP chip and characterized phenotypically during four seasons for a fine QTL mapping analysis. Based on the molecular diversity of p3_VvAGL11 alleles, we redesigned this marker, and based on the causal SNP, we developed a qPCR-HRM marker for high-throughput and a Tetra-ARMS-PCR for simple predictive analyses. Up to 10 new QTLs were identified that describe the complex nature of seedlessness, corresponding to small but stable effects. The positive predictive value, based on VvAGL11 alone (0.647), was improved up to 0.814 when adding three small-effect QTLs in a multi-QTL additive model as a proof of concept. The new SSR, 5U_VviAGL11, is more informative and robust, and easier to analyze. However, we demonstrated that the association can be lost by intragenic recombination and that the e7_VviAGL11 SNP-based marker is thus more reliable and decreases the occurrence of false positives. This study highlights the bases of prediction failure based solely on a major gene and a reduced set of candidate genes, in addition to opportunities for molecular breeding following further and larger validation studies.
- Published
- 2020
21. FT-NIR Analysis of Intact Table Grape Berries to Understand Consumer Preference Driving Factors
- Author
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Rocco Perniola, Antonio Domenico Marsico, Teodora Basile, Maria Francesca Cardone, and Donato Antonacci
- Subjects
R software ,Health (social science) ,correlogram ,PLS ,Plant Science ,Berry ,lcsh:Chemical technology ,01 natural sciences ,Health Professions (miscellaneous) ,Microbiology ,Sensory analysis ,Spearman's rank correlation coefficient ,Article ,sensory analysis ,0404 agricultural biotechnology ,lcsh:TP1-1185 ,Food science ,Sugar ,Chemical composition ,Mathematics ,PCA ,010401 analytical chemistry ,Table grape ,NIR ,04 agricultural and veterinary sciences ,Sweetness ,040401 food science ,0104 chemical sciences ,Food Science - Abstract
Fourier-transform near infrared spectroscopy (FT-NIR) is a technique used in the compositional and sensory analysis of foodstuffs. In this work, we have measured the main maturity parameters for grape (sugars and acids) using hundreds of intact berry samples to build models for the prediction of these parameters from berries of two very different varieties: &ldquo, Victoria&rdquo, and &ldquo, Autumn Royal&rdquo, Together with the chemical composition in terms of sugar and acidic content, we have carried out a sensory analysis on single berries. Employing the models built for sugars and acids it was possible to learn the sweetness and acidity of each berry before the destructive sensory analysis. The direct correlation of sensory data with FT-NIR spectra is difficult, therefore, spectral data were exported from the spectrometer built-in software and analyzed with R software using a statistical analysis technique (Spearman correlation) which allowed the correlation of berry appreciation data with specific wavelengths that were then related to sugar and acidic content. In this article, we show how it is possible to carry out the analysis of single berries to obtain data on chemical composition parameters and consumer appreciation with a fast, simple, and non-destructive technique with a clear advantage for producers and consumers.
- Published
- 2020
22. A chemometric approach to identify the grape cultivar employed to produce nutraceutical fruit juice
- Author
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Rocco Perniola, Carlo Bergamini, Tiziana Dipalmo, Maria Lisa Clodoveo, Pasquale Crupi, and Donato Antonacci
- Subjects
chemistry.chemical_classification ,Table grape ,General Chemistry ,Biology ,Biochemistry ,Industrial and Manufacturing Engineering ,Anthocyanidins ,chemistry.chemical_compound ,Nutraceutical ,Flavonols ,chemistry ,Polyphenol ,Food science ,Cultivar ,Kaempferol ,Quercetin ,Food Science ,Biotechnology - Abstract
Apulian farmers have begun to explore the potential of grape juice as an alternative method to increase the profitability and stability of their farming operations. Grape juice obtained from table cultivar rich in polyphenols are considered value-added food products, so producers are interested in identifying an analytical method that permits (1) to quantify the phenolic composition, in order to select the richer variety of antioxidant substance, and (2) to trace the grape cultivar, in order to have a tool to protect their nutraceutical product from imitations. In this work, a chemometric approach, consisting of the comprehensive application of two-way full-factorial MANOVA, hierarchical cluster (HCA), and principal component analyses (PCA), is proposed as tool to differentiate seven table grape varieties (white, ‘Carati,’ ‘Sugraone,’ and ‘Italia’ and colored ‘Michele Palieri,’ ‘Summer Royal,’ ‘Autumn Royal,’ and ‘Crimson Seedless’) actually used to produce grape juice in Apulia. Anthocyanins were the main flavonoids in the colored grapes, and highly significant quantitative differences (F cultivar × vintage = 25,461; p
- Published
- 2015
23. Ampelometric Leaf Trait and SSR Loci Selection for a Multivariate Statistical Approach in Vitis vinifera L. Biodiversity Management
- Author
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Carlo Bergamini, Rocco Perniola, Marica Gasparro, Rosalinda Genghi, Vittorio Alba, and Donato Antonacci
- Subjects
Genotype ,business.industry ,Quantitative Trait Loci ,Bioengineering ,Heritability ,Biology ,Applied Microbiology and Biotechnology ,Biochemistry ,CHAID ,Ampelography ,Biotechnology ,Plant Leaves ,Statistics ,Principal component analysis ,Trait ,Microsatellite ,Vitis ,business ,Vitis vinifera ,Molecular Biology ,Algorithms ,Selection (genetic algorithm) ,Microsatellite Repeats - Abstract
High estimated heritability values were recently revealed for mature leaf traits in grape (Vitis vinifera L.), thus redeeming ampelography in the era of molecular markers. The "Organisation Internationale de la Vigne et du Vin (OIV)" set a list of hundreds of descriptors for grapevine in order to standardize ampelographic and ampelometric scores. Therefore, the selection and reduction of the number of OIV codes can represent a major goal for leaner biodiversity assessment studies. The identification of ampelometric traits associated with grape diversity allows to construct Classification Trees with chi squared automatic interaction detection (CHAID) algorithm, a stepwise model-fitting method that produces a tree diagram in which at each step the sample pool is splitted based on the independent variables statistically different for the dependent variable. A collection of 100 table and wine grapevines (Vitis vinifera L.) was characterized and evaluated by means of six microsatellites and twenty-two ampelometric traits on mature leaves. Nine ampelometric traits were selected by principal component analysis and employed to build the classification trees based on CHAID algorithm. The strategy can represent an effective tool for grape biodiversity management, right allocations, and identification of new grape genotypes, implemented by a further microsatellite investigation only when unsolved cases occur, allowing faster and cheaper results.
- Published
- 2015
24. Morphological Variability in Leaves and Molecular Characterization of Novel Table Grape Candidate Cultivars (Vitis vinifera L.)
- Author
-
Maria Francesca Cardone, Rocco Perniola, Rosalinda Genghi, Marica Gasparro, Carlo Bergamini, Vittorio Alba, and Donato Antonacci
- Subjects
Genotype ,Dendrogram ,Table grape ,Genetic Variation ,Bioengineering ,Biology ,Applied Microbiology and Biotechnology ,Biochemistry ,Ampelography ,Plant Leaves ,Italy ,Plant morphology ,Principal component analysis ,Botany ,Microsatellite ,Vitis ,Cultivar ,Molecular Biology ,Microsatellite Repeats ,Biotechnology - Abstract
The present work report the characterization of twenty-one table grapes candidate cultivars plus five registered ones included as reference, by means of 47 ampelographic traits, 23 ampelometric measurements and six microsatellite loci. The final goal of the research was to analyse the possibility of reducing the number of morphological and molecular tools required for a precise and effective description of a grape genotype or cultivar. This would be of great help for future biodiversity description on a larger sample of more than 300 table grapes accessions today grown at the 'Consiglio per la Ricerca e la sperimentazione in Agricoltura (C.R.A.)-Unità di ricerca per l'uva da tavola e la vitivinicoltura in ambiente mediterraneo (Bari-Italy)'. OIV ampelographic traits showed a clear distinction among all twenty-six genotypes analysed, suggesting the relevant morphological variability investigated. Principal component analysis based on ampelometric traits revealed main veins ON(3), ON(4) and O(3)N(4); ratios between main veins; angles between main veins and of petiolar sinus, to be the most effective records in differentiating cultivars, for a total variation of 69.9 % described by the first three components. Molecular analysis based on six microsatellite loci was performed on all genotypes, providing a detailed molecular profile and a dendrogram of genetic similarity, in which all genotypes were clearly distinguishable. Finally, with the goal of using the minimum possible number of markers to differentiate genotypes, microsatellites VVMD5 and VVMD27 were selected to be sufficient to distinguish among all the candidate cultivars included in the analysis, representing a possible 'step by step' approach when a molecular characterization has to be undertaken on a large number of genotypes, by first testing few markers and increasing their number only if necessary.
- Published
- 2014
25. Phenotypic evaluation of segregant population derived by crossing table grape varieties
- Author
-
Riccardo Velasco, Donato Antonacci, Maria Francesca Cardone, Lucia R Forleo, Matteo Velenosi, Carlo Bergamini, Rocco Perniola, Antonio Domenico Marsico, and A. L’Abbate
- Subjects
education.field_of_study ,Environmental Engineering ,lcsh:QP1-981 ,Breeding program ,fungi ,Table grape ,Population ,lcsh:QR1-502 ,Cold storage ,Berry ,Biology ,Vineyard ,lcsh:Microbiology ,lcsh:Physiology ,Industrial and Manufacturing Engineering ,Embryo rescue ,Horticulture ,lcsh:Zoology ,lcsh:QL1-991 ,education - Abstract
In the CREA-Viticoltura ed Enologia, Lab of Turi, during the last ten years, we started a breeding program for table grapes to obtain new seedless varieties, by using conventional breeding by crossings, and embryo rescue techniques. Other than seedlessness, additional targets for this breeding program are: the possibility of extending the harvesting period, the attitude to cold storage, transport and shelf-life, resistance to diseases both on the plant and in post-harvest conditions, the good productivity, the quality of the grapes, the easy cultivation management of the vineyard (reduced water, nutritional requirements, etc ...). More than 10.000 new genotypes have been obtained through the use of over 20 table grape varieties and more than 18 different crossing combinations. The following characteristics have been observed on these individuals for more than three years: berry color, length and weight cluster, average berry weight, sugars, pH, acidity, class of seedlessness, floral morphology, resistance to diseases. The results of these activities revealed that some combinations have better performance among all. In the near future, this activity will allow to focus on parental genotypes able to provide individuals with the best desired traits.
- Published
- 2019
26. Evoluzione della tecnica d'innesto su barbatella radicata
- Author
-
Antonio Domenico Marsico, Rocco Perniola, and Lucia R Forleo
- Subjects
Environmental Engineering ,lcsh:QP1-981 ,lcsh:Zoology ,lcsh:QR1-502 ,lcsh:QL1-991 ,Humanities ,lcsh:Microbiology ,lcsh:Physiology ,Industrial and Manufacturing Engineering - Abstract
In viticoltura, per la realizzazione di nuovi impianti sono utilizzate piante bimembri ottenute in vivaio o direttamente in campo. Negli ultimi anni, allo scopo di ottenere rapidamente nuovi impianti, si sta diffondendo la tecnica dell'innesto su barbatella radicata. Tale tecnica, realizzata in vivaio, prevede l'innesto a tavolino delle marze della varietà scelta su barbatelle radicate di portainnesto di specie di Vitis americane. Dopo un periodo di forzatura e ambientamento in vivaio, le piantine vengono messe a dimora in campo, nello stesso anno di realizzazione dell'innesto. Al fine di valutare tale tecnica, presso il CREA – Viticoltura ed enologia sede di Turi, è stata sperimentata la possibilità di utilizzare due differenti tipologie d'innesto su diversi portinnesti. Per assicurare una buona resistenza del punto d'innesto ed una buona protezione dalla contaminazione e dalla disidratazione è stato utilizzato un materiale di saldatura costituito da una pellicola elastica e semitrasparente. La sperimentazione è stata condotta per due anni, su 12 varietà di uve da tavola di recente costituzione. I risultati hanno mostrato buoni attecchimenti e saldatura dei bionti. Alcune differenze sono state riscontrate sui portinnesti utilizzati e sui tempi di germogliamento dopo l'innesto.
- Published
- 2019
27. Validation Assay of p3_VvAGL11 Marker in a Wide Range of Genetic Background for Early Selection of Stenospermocarpy in Vitis vinifera L
- Author
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Donato Antonacci, Lucia R Forleo, Rosalinda Genghi, Rocco Perniola, Angelo Anaclerio, Arianna Pichierri, Carlo Bergamini, Angelo Raffaele Caputo, Antonio Blanco, Cinzia Montemurro, Maria Francesca Cardone, and Vittorio Alba
- Subjects
Genetic Markers ,Germplasm ,DNA, Plant ,Genotype ,Bioengineering ,Genomics ,Breeding ,Biology ,Applied Microbiology and Biotechnology ,Biochemistry ,Negative selection ,Vitis ,Allele ,Molecular Biology ,Selection (genetic algorithm) ,Molecular breeding ,Genetics ,business.industry ,fungi ,Reproducibility of Results ,food and beverages ,Marker-assisted selection ,Biotechnology ,Phenotype ,Genetic marker ,Fruit ,Seeds ,business - Abstract
DNA markers technology, derived from research in molecular biology and genomics, offers great promise for plant breeding, allowing the "molecular breeding" via marker-assisted selection. Grapevine genomic resources allowed, in recent years, the characterization at molecular level of genes involved in interesting phenotypes such as stenospermocarpic seedlessness, a trait really appreciated by consumers. Recent studies in table grapes revealed that the VvAGL11 gene, member of the D-lineage MADS-box family, controls the ovule identity, and thus potentially playing an important role in stenospermocarpy. Intragenic markers of VvAGL11 have been found and tested for breeding purposes. In the present paper, we describe an in deep assay on a total of 475 genotypes derived by our own grape germplasm and seeded × seedless crosses F1 offspring, to evaluate and verify the "diagnostic" power of VvAGL11 in marker-assisted selection. We found only 8/475 that were seeded and carried the seedless-associated allele in the STS p3_VvAGL11. However, and most importantly, there were no seedless varieties without such allele. We validated the marker as a 100 % effective tool for early negative selection of stenospermocarpy in Vitis vinifera L. crosses.
- Published
- 2013
28. Genomics technologies to study structural variations in the grapevine genome
- Author
-
Claudia Rita Catacchio, Pietro D'Addabbo, Fabio Anaclerio, Giorgia Chiatante, Giuliana Giannuzzi, Mario Ventura, Carlo Bergamini, Maria Francesca Cardone, Donato Antonacci, Rocco Perniola, Annamaria Marra, and Can Alkan
- Subjects
Molecular breeding ,Genetics ,Environmental Engineering ,lcsh:QP1-981 ,lcsh:QR1-502 ,Genomics ,Single-nucleotide polymorphism ,Biology ,Genome ,Phenotype ,Industrial and Manufacturing Engineering ,lcsh:Microbiology ,lcsh:Physiology ,Structural variation ,Evolutionary biology ,lcsh:Zoology ,Copy-number variation ,lcsh:QL1-991 ,Gene - Abstract
Grapevine is one of the most important crop plants in the world. Recently there was great expansion of genomics resources about grapevine genome, thus providing increasing efforts for molecular breeding. Current cultivars display a great level of inter-specific differentiation that needs to be investigated to reach a comprehensive understanding of the genetic basis of phenotypic differences, and to find responsible genes selected by cross breeding programs. While there have been significant advances in resolving the pattern and nature of single nucleotide polymorphisms (SNPs) on plant genomes, few data are available on copy number variation (CNV). Furthermore association between structural variations and phenotypes has been described in only a few cases. We combined high throughput biotechnologies and bioinformatics tools, to reveal the first inter-varietal atlas of structural variation (SV) for the grapevine genome. We sequenced and compared four table grape cultivars with the Pinot noir inbred line PN40024 genome as the reference. We detected roughly 8% of the grapevine genome affected by genomic variations. Taken into account phenotypic differences existing among the studied varieties we performed comparison of SVs among them and the reference and next we performed an in-depth analysis of gene content of polymorphic regions. This allowed us to identify genes showing differences in copy number as putative functional candidates for important traits in grapevine cultivation.
- Published
- 2016
29. Study of main grapevine viruses transmission in breeding programs
- Author
-
Marica Gasparro, Rosa Anna Milella, Donato Antonacci, Angelo Raffaele Caputo, Rocco Perniola, Vittorio Alba, and Lucia R Forleo
- Subjects
0106 biological sciences ,0301 basic medicine ,Environmental Engineering ,lcsh:QP1-981 ,Transmission (medicine) ,Table grape ,lcsh:QR1-502 ,food and beverages ,Biology ,biology.organism_classification ,01 natural sciences ,Crossbreed ,Industrial and Manufacturing Engineering ,Virus ,lcsh:Microbiology ,lcsh:Physiology ,03 medical and health sciences ,Horticulture ,030104 developmental biology ,Seedling ,lcsh:Zoology ,Cultivar ,lcsh:QL1-991 ,010606 plant biology & botany - Abstract
A number of studies have established that vertical transmission of viruses is an important phenomenon affecting a wide range of viruses, especially as a result of breeding programs. Nevertheless, the presence of virus in a seed, does not always lead to seedling infection. In grapevine, seed transmission has been reported for many nepoviruses, but little is known about the other virus associated diseases such as Leafroll, Rugose wood complex and Fleck diseases, that are considered by Italian legislation on certification of grapevine propagation material, together with infectious degeneration. Thus, the aim of this study is monitoring the virological condition of seedlings obtained by crosses between infected parentals, analyzing the sanitary status of seedlings after the transfer in experimental fields. In this study, 50 progeny plants from three different traditionally crossbreed seeded and seedless table grape cultivars were investigated for the detection of nine grapevine viruses. It was observed that the viral state of parentals was quite compromised, while viruses were not detected in any of the 150 progeny plants, demonstrating that the main grapevine viruses are at low risk for seed transmission. These results could make it possible to reduce the precautions to obtain propagation material free from infectious diseases and also suggest to consider reproduction as a new procedure of sanitation in breeding programs.
- Published
- 2016
30. Sangiovese and Its Offspring in Southern Italy
- Author
-
Pasquale Crupi, Maria Francesca Cardone, Donato Antonacci, Carlo Bergamini, Rocco Perniola, Angelo Raffaele Caputo, and Marica Gasparro
- Subjects
Vine ,Genotype ,Bioengineering ,Applied Microbiology and Biotechnology ,Biochemistry ,Archaeology ,Ampelography ,Molecular analysis ,Geography ,Italy ,Botany ,Vitis ,Molecular Biology ,Biotechnology - Abstract
This paper demonstrates the importance of different approaches such as ampelography, historical researches, and molecular analysis to reveal direct parent-child relationship. The aim of this paper was to highlight the degree of relationship to five varieties spread in southern Italy, through ampelographic and molecular characterization: Sangiovese, Mantonico di Bianco, Gaglioppo di Cirò, Mantonicone, and Nerello Mascalese. Molecular characterization was carried out through 52 SSR molecular markers, showing that Sangiovese and Mantonico di Bianco are the parents of Gaglioppo di Cirò, Mantonicone, and Nerello Mascalese. Ampelographic description was performed using the method developed by the Organisation Internationale de la Vigne et du Vin. This analysis identifies three distinct groups: the first brings together Sangiovese and the two offspring Nerello Mascalese and Gaglioppo di Cirò, while Mantonico di Bianco and Mantonicone are positioned at a distance from the first and between them. Using molecular characterization, supported by the ampelographic one, we showed that Gaglioppo di Cirò, Mantonicone, and Nerello Mascalese, three varieties recovered in the southern regions of Italy, such as Calabria and Sicily, originated by the cross between a nationally spread grape variety as Sangiovese and a Calabria autochthonous vine as Mantonico di Bianco.
- Published
- 2012
31. Evidences for an Alternative Genealogy of ‘Sangiovese’
- Author
-
Rocco Perniola, Angelo Raffaele Caputo, Maria Francesca Cardone, Marica Gasparro, Carlo Bergamini, and Donato Antonacci
- Subjects
DNA, Plant ,Bioengineering ,Biology ,Applied Microbiology and Biotechnology ,Biochemistry ,Genealogy ,Molecular analysis ,Gene Frequency ,Genetic Loci ,Databases, Genetic ,Kinship ,Microsatellite ,Vitis ,Molecular Fingerprinting ,Vitis vinifera ,Molecular Biology ,Genome, Plant ,Microsatellite Repeats ,Biotechnology ,Research method - Abstract
Two different hypothesis for the parentage of 'Sangiovese', the most important and widespread Italian winegrape, have been proposed by some previous studies. We screened our grapevine collection, mostly comprising south Italian cultivars collected to preserve biodiversity, to asses kinships. Surprisingly we found two previously unreported candidate parents for 'Sangiovese'. The first putative parent is 'Ciliegiolo' a well know variety already addressed as relative of 'Sangiovese'; the second putative parent is 'Negrodolce', an old local variety we recovered and was considered lost during the last century. In order to obtain a stronger statistical support for this new kinship, we tested seventy different microsatellite markers but only 57 were found reliable. The new proposed parentage stood well even with such a in depth molecular analysis whereas only one discrepancy was found in one of the 57 microsatellite marker analyzed. This discrepancy is certainly due to a null-allele and therefore it should not impair our hypothesis but it points out limits of the microsatellites profiling as a pedigree research method considering that this is the third different kinship proposed so far for 'Sangiovese'. Thus in this article, by means of detailed molecular fingerprinting, we provide a completely new strong evidence for a south Italian origin of 'Sangiovese' and we discuss our findings comparing our data with those previously reported by other authors.
- Published
- 2012
32. HPLC-DAD-ESI-MS analysis of flavonoid compounds in 5 seedless table grapes grown in Apulian Region
- Author
-
Donato Antonacci, Rosa Anna Milella, Pasquale Crupi, Antonio Coletta, Rocco Perniola, Marica Gasparro, and Rosalinda Genghi
- Subjects
Mediterranean climate ,Flavonoid ,Biology ,Catechin ,Anthocyanins ,chemistry.chemical_compound ,Flavonols ,Glucosides ,Botany ,Biflavonoids ,Proanthocyanidins ,Vitis ,Kaempferols ,Procyanidin B1 ,Procyanidin B2 ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Flavonoids ,Table grape ,Monosaccharides ,Galactosides ,Horticulture ,chemistry ,Italy ,Linear Models ,Quercetin ,Food quality ,Food Science - Abstract
UNLABELLED Flavonoids present in skin extracts of red seedless table grape varieties Summer Royal, Autumn Royal, and Crimson, and white seedless varieties Carati and Thompson were analyzed by HPLC-DAD-MS, in 3 y of study (2006 to 2008). The anthocyanins, delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside (with their corresponding p-coumaroyl derivatives), peonidin-3-O-glucoside, and malvidin-3-O-glucoside (with their corresponding acetyl, caffeoyl, and p-coumaroyl derivatives) were found. In addition the flavonols quercetin-3-O-glucuronide, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, kaempferol-3-O-galactoside, and the flavan-3-ols procyanidin B1, procyanidin B2, and catechin were also detected. Anthocyanins were the main flavonoids in red grapes ranging from 24 (Crimson) to 500 (Summer Royal) mg/kg fresh weight of grapes; consistent levels of flavonols and flavan-3-ols were also quantified in all varieties. To determine the effective climatic influence on flavonoids content in field conditions, viticultural practices have been developed, that could exclude the effects of direct solar radiation from confounding the assessment of those related to thermal conditions alone. A strong positive correlation was determined between flavonoids and temperature data that seem to be responsible for the difference of these metabolites along the years; furthermore, it has been possible to define a linear relationship (R(2) = 0.6871, P = 0.0057) between thermal amplitude and total flavonoids values in the red grapes. PRACTICAL APPLICATION Grapes are economically the most important fruit species in the world and approximately 30% of its production is used as fresh fruit. Because of the very important role of flavonoids in food quality as well as their health-promoting properties, and considering that our experiments were performed along 3 consecutive years, gathered results in this research are quite promising to give a useful information on the flavonoid contents and their evolution in 5 seedless table grapes that are widespread in Mediterranean regions but also in California and South America, and are grown in a viticultural climate (Apulia, South Italy) very close to some regions of Spain, Turkey, Tunisia, and Israel.
- Published
- 2012
33. Erratum to: Morphological Variability in Leaves and Molecular Characterization of Novel Table Grape Candidate Cultivars (Vitis vinifera L.)
- Author
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Vittorio Alba, Carlo Bergamini, Maria Francesca Cardone, Marica Gasparro, Rocco Perniola, Rosalinda Genghi, and Donato Antonacci
- Subjects
Bioengineering ,Molecular Biology ,Applied Microbiology and Biotechnology ,Biochemistry ,Biotechnology - Published
- 2014
34. Role of the Physical Elicitors in Enhancing Postharvest Antioxidant Capacity of Table Grape cv Redglobe (Vitis vinifera L.)
- Author
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Donato Antonacci, Rosa Anna Milella, Rocco Perniola, Pasquale Crupi, and Arianna Pichierri
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Vintage ,Antioxidant ,Chemistry ,Abiotic stress ,medicine.medical_treatment ,fungi ,Table grape ,food and beverages ,Antioxidant capacity ,Polyphenol ,Botany ,medicine ,Postharvest ,Food science ,Vitis vinifera - Abstract
Polyphenols, such as anthocyanins, are secondary metabolites produced in plants which can play an important health-promoting role considering their strong correlation with antioxidant capacity. The biosynthesis of these compounds generally increases as a response to biotic or abiotic stress, therefore, in order to achieve as high phenolic accumulation as possible, the interactive effects of storage conditions (temperature and time) and postharvest ultraviolet irradiation (UV-C) on total polyphenols (TPP) and total anthocyanins (TA) content, as well as oxygen radical antioxidant capacity (ORAC), in postharvest “Redglobe” table grape variety were investigated in 2011 vintage. Gathered findings showed that UV-C exposure ranging from 1 to 3 min (0.8-2.4 KJ m-2) positively influence the TA level (with an increase almost twice higher than the control) during a storage time longer than 48 h, independently from the storage temperature; while, with regard to TPP and ORAC, a progressive increment of their values (roughly from 2 to 4 folds higher than the control) up to 120 h of storage was observed, in particular in Redglobe grapes irradiated for 1 min and stored at 4 °C. Responding to the rising consumers needs to have foods that support and promote health, this research demonstrated that the postharvest simultaneous application of physical elicitors can lead to grapes with enhanced antioxidant properties, within normal conditions of market commercialization. The practical relevance of this finding is evident considering that grapes are economically the most important fruit species in the world and approximately 30% of its production is used as fresh fruit.
- Published
- 2014
35. P62 - Vitis vinifera L. new crossings tolerance to downy mildew.
- Author
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Maggiolini, Flavia Angela Maria, Velasco, Riccardo, Ventura, Mario, Bergamini, Carlo, Cardone, Maria Francesca, Rocco, Perniola, D'Amico, Margherita, Forleo, Lucia Rosaria, Salerno, Antonella, and Marsico, Antonio Domenico
- Subjects
DOWNY mildew diseases ,VITIS vinifera ,DISEASE susceptibility ,AUTUMN ,GENE expression ,DISEASE incidence ,TRANSCRIPTOMES ,LIQUID nitrogen - Abstract
Although the high susceptibility of Vitis vinifera to the fungus Plasmopara viticola, several elements in this field suggest a variety-specific response to this oomycete infection. To date, not much is known about the degree of susceptibility to downy mildew segregates in populations derived from breeding of V. vinifera varieties with different degrees of susceptibility. Thus, CREA-Viticulture and Enology of Turi (BA) breeding program, among its goals, focuses on the identification of new Vitis genotypes showing more tolerance to this biotic stress. Starting from 2021, different genotypes belonging to two segregating populations, Inzolia imperiale x Autumn royal seedless and N22/050 ('Red globe' x 'Regal seedless') x 'Melissa', have been phenotypically evaluated for their differential response to P. viticola infection. In detail, a leaf disc assay was performed, and each leaf disc has been inoculated with 50 µL of a suspension of P. viticola zoosporangia. Five days after the inoculation the incidence of the disease was calculated as a percentage ratio between the number of leaf discs showing symptoms and the total number of tested discs. Furthermore, the severity of the infection was evaluated by using an empirical 0-to-4 rating scale and for each genotype the Mckinney's index was calculated. Noteworthy, data show that at least 10% of the tested genotypes, for each segregating population, are highly tolerant to the fungus infection. Among the Inzolia imperiale x Autumn royal seedless tested individuals, 18 out of 113 genotypes showing different responses to the infection were selected and the leaf disc assay was repeated on them to confirm their degree of tolerance/susceptibility to the disease. Moreover, to further investigate the genotypes response, some infected leaf discs from each genotype were collected and frozen in liquid nitrogen at different time points after inoculation and stored for transcriptomics studies aiming to evaluate differences in genes expression with respect to the degree of tolerance registered for each sample. The tolerance to the P. viticola infection of these genotypes will be tested also in the field in the next future and other commercial characteristic will be evaluated. We believe this work will lay the ground for providing in the next future new cultivars of V. vinifera, whose management will allow a lower environmental impact. [ABSTRACT FROM AUTHOR]
- Published
- 2022
36. The molecular characterization by SSRs reveals a new South Italian kinship and the origin of the cultivar Uva di Troia
- Author
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Angelo Raffaele Caputo, Maria Francesca Cardone, Carlo Bergamini, Marica Gasparro, R. Pepe, Rocco Perniola, and Donato Antonacci
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0106 biological sciences ,0301 basic medicine ,Germplasm ,Bombino bianco ,Synonym ,Biodiversity ,Pedigree chart ,01 natural sciences ,03 medical and health sciences ,Quagliano ,Botany ,Kinship ,Uva di Troia ,Bombino nero ,Microsatellites ,Multidisciplinary ,biology ,Research ,biology.organism_classification ,Genealogy ,030104 developmental biology ,Impigno ,Microsatellite ,Taxonomy (biology) ,Grapevine ,010606 plant biology & botany - Abstract
Vitis vinifera L. varieties were spread through cuttings following historic migrations of people, trades, or after biological crises due to pests outbreaks. Some today’s varieties could be more than a 1000 years old and, although over the centuries these varieties generated most of the remaining cultivars, their origin could be impossible to track back. The Italian grapevine biodiversity is one of most important, most likely due to its strategic position in the middle of the Mediterranean sea. Unravelling of its structure is challenging because of its complexity and the lack of historical documentation. In this paper molecular data are compared with historical documentations. Simple Sequence Repeats fingerprinting are molecular markers best suited to investigate genetic relationships and identify pedigrees. South-Italian germplasm was studied with 54 nuclear microsatellites. A family was identified, consisting of two parents and three siblings and further genetically characterized with six nuclear and five chloroplast microsatellites and described with ampelographic and phylometric analysis. Although these latter were not informative for the kinship identification. The common Bombino bianco was the female parent and the previously unknown Uva rosa antica was the male parent. Bombino nero, Impigno and the popular Uva di Troia, all typical of the south-east Italy, were the offspring. Further research showed that the Uva rosa antica was a synonym of Quagliano and Bouteillan noir, both minor varieties. Quagliano was considered to be autochthonous of some alpine valleys in the north-west of Italy and Bouteillan noir is a neglected variety of Vancluse in France. This finding uncovers the intricate nature of Italian grape cultivars, considered peculiar of an area, but possibly being the remains of ancient latin founding varieties. Consequently, intriguing new hypotheses are discussed and some conclusions are drawn, based on the peculiar geographical origin of the parents, on the distribution of the offspring, on the chance of a single, and perhaps intentional, crossing event.
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