Search

Your search keyword '"Rodelas, Belén"' showing total 43 results
Start Over Author "Rodelas, Belén"
43 results on '"Rodelas, Belén"'

18. Simplified engineering design towards a competitive lipid-rich effluents valorization

Author

Argiz Montes, Lucía, Val del Río, María Ángeles, Correa Galeote, David, Rodelas, Belén, Mosquera Corral, Anuska, Universidade de Santiago de Compostela. Departamento de Enxeñaría Química, and Universidade de Santiago de Compostela. Instituto Interdisciplinar de Tecnoloxías Ambientais (CRETUS)

Subjects
Environmental Engineering, Mixed microbial culture, Polyhydroxyalkanoates, Industrial Waste, General Medicine, Management, Monitoring, Policy and Law, Waste lipids, Fatty Acids, Volatile, Polyhydroxyalkanoate, Triacylglyceride, Carbon, Bioreactors, Bioprocess, Waste Management and Disposal
Abstract

Medium- and long-chain fatty acids and glycerol contained in the oily fraction of many food-industry effluents are excellent candidates to produce biobased high-value triacylglycerides (TAGs) and polyhydroxyalkanoates (PHAs). The typical process configuration for TAGs recovery from lipid-rich streams always includes two steps (culture enrichment plus storage compounds accumulation) whereas, for PHAs production, an additional pretreatment of the substrate for the obtainment of soluble volatile fatty acids (VFAs) is required. To simplify the process, substrate hydrolysis, culture enrichment, and accumulation (TAG and PHA storage) were coupled here in a single sequencing batch reactor (SBR) operated under the double growth limitation strategy (DGL) and fed in pulses with industrial waste fish oil during the whole feast phase. When the SBR was operated in 12 h cycles, it was reached up to 51 wt % biopolymers after only 6 h of feast (TAG:PHA ratio of 50:51; 0.423 CmmolBIOP/ CmmolS). Daily storage compound production was observed to be over 25% higher than the reached when enrichment and accumulation stages were carried in separate operational units. Increasing the feast phase length from 6 to 12 h (18 h cycle) negatively affected the DGL strategy performance and hence system storage capacity, which was recovered after also extending the famine phase in the same proportion (24 h cycle). Besides, the carbon influx during the feast phase was identified as a key operational parameter controlling storage compounds production and, together with the C/N ratio, culture selection. The different cycle configurations tested clearly modulated the total fungal abundances without no significant differences in the size of the bacterial populations. Several PHA and TAG producers were found in the mixed culture although the PHA and TAG productions were poorly associated with the increased relative abundances (RAs) of specific operational taxonomic units (OTUs)., Spanish Government (AEI, Spain) through the TREASURE project CTQ 2017-83225-C2-1-R operative program FSE Galicia 2014-2020, European Commission

Published
2022

41. Influence of linear alkylbenzene sulfonate (LAS) on the structure of Alphaproteobacteria, Actinobacteria, and Acidobacteria communities in a soil microcosm.

Author

del Mar Sánchez-Peinado, Ma., González-López, Jesús, Ma. Victoria Martínez-Toledo, Pozo, Clementina, and Rodelas, Belén

Subjects
ALKYLBENZENE sulfonates, MICROCOSM & macrocosm, ACTINOBACTERIA, BACTERIA, DENATURING gradient gel electrophoresis
Abstract

The article presents a study which explores the application of microcosm system to evaluate the effects of linear alkylbenzene sulfonate (LAS) on the community structure of Alphaproteobacteria, Actinobacteria, and Acidobacteria in the soil. The bacteria were analyzed through temperature gradient gel electrophoresis (TGGE). It was found that LAS concentration significantly influences the development of bacterial community structure.

Published
2010
Full Text
View/download PDF

42. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR).

Author

Maza-Márquez P, Aranda E, González-López J, and Rodelas B

Subjects
DNA Primers genetics, DNA, Environmental genetics, DNA, Fungal genetics, Fungi genetics, Microbiota genetics, RNA, Ribosomal, 18S genetics, Species Specificity, DNA, Environmental isolation & purification, DNA, Fungal isolation & purification, Real-Time Polymerase Chain Reaction methods, Sewage microbiology, Water Purification
Abstract

Assessment of the abundance of fungi in environmental samples by quantitative PCR (qPCR) of community DNA is often a difficult task due to biases introduced during PCR amplification, resulting from the differences associated with length polymorphism and the varying number of copies of the rRNA operon among fungal species, the lack of specificity of the primers targeting the different regions of the rRNA operon, or their insufficient coverage of the fungal lineages. To overcome those limitations, it is crucial to test and select the specific primers sets which provide the more accurate approximation to the quantification of the targeted fungal populations in a given set of samples. Fungi are a significant fraction of the microbiota in wastewater treatment plants (WWTPs), but the activated sludge microbial communities comprise many other eukaryotic microorganisms whose molecular markers are often coamplified by primers initially designed as fungal-specific. Here, the use of the FungiQuant primer set is recommended for the quantification of fungal molecular markers (18S rRNA genes) by qPCR in activated sludge samples and the full protocol is described.

Published
2020
Full Text
View/download PDF

43. Removal of organic load from olive washing water by an aerated submerged biofilter and profiling of the bacterial community involved in the process.

Author

Pozo C, Rodelas B, Martínez-Toledo MV, Vílchez R, and González-López J

Subjects
Aerobiosis, Bacteria metabolism, Biofilms growth & development, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Electrophoresis, Polyacrylamide Gel, Food Industry, Genes, rRNA, Industrial Waste, Nucleic Acid Denaturation, Olea, Oxygen Consumption, Phylogeny, Polyphenols, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Bacteria classification, Bacteria isolation & purification, Filtration methods, Flavonoids metabolism, Phenols metabolism, Water Purification methods
Abstract

The present work aims to use a biofilter technology (aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water (OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days (BOD5) and chemical oxygen demand (COD), decreased markedly (up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced (around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the alpha-subclass of Proteobacteria, and often branched in the periphery of bacterial genera commonly present in soil (Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

Published
2007

Catalog

Books, media, physical & digital resources
See catalog results