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Abstract Reproducibility of computational research is often challenging despite established guidelines and best practices. Translating these guidelines into practical applications remains difficult. Here, we present ENCORE, an approach to enhance transparency and reproducibility by guiding researchers in how to structure and document a computational project. ENCORE builds on previous efforts in computational reproducibility and integrates all project components into a standardized file system structure. It utilizes pre-defined files as documentation templates, leverages GitHub for software versioning, and includes an HTML-based navigator. ENCORE is designed to be agnostic to the type of computational project, data, programming language, and ICT infrastructure, and does not rely on specific software tools. We also share our group’s experience using ENCORE, highlighting that the most significant challenge to the routine adoption of approaches like ours is the lack of incentives to motivate researchers to dedicate sufficient time and effort to ensure reproducibility.

IntroductionThis systematic review and meta-analysis present a comprehensive evaluation of paper-based microfluidic devices, focusing on their applications in immunoassays. These devices are emerging as innovative solutions to democratize access to diagnostic technologies, especially in resource-limited settings. Our review consolidates findings from diverse studies to outline advancements in paper-based microfluidic technology, including design intricacies and operational efficacy. Key advantages such as low cost, portability, and ease of use are highlighted.Materials and MethodsThe review categorizes literature based on the design and operational nuances of these diagnostic tools, exploring various methodologies, fabrication techniques, detection methods, and applications, particularly in protein science. The meta-analysis extends to the diverse applications of these technologies, providing a framework for classifying and stratifying their uses in diagnostics.Results and discussionNotable findings include a critical analysis of performance metrics, such as sensitivity and specificity. The review addresses challenges, including the need for further validation and optimization for broader clinical applications. A critical discussion on the validation processes, including cross-validation and rigorous control testing, is provided to ensure the robustness of microfluidic devices. This study offers novel insights into the computational strategies underpinning these technologies and serves as a comprehensive roadmap for future research, potentially broadening the impact across the protein science universe.

Acoustic management is very important for detecting possible events in the context of a smart environment (SE). In previous works, we proposed a reflective middleware for acoustic management (ReM-AM) and its autonomic cycles of data analysis tasks, along with its ontology-driven architecture. In this work, we aim to develop an emotion-recognition system for ReM-AM that uses sound events, rather than speech, as its main focus. The system is based on a sound pattern for emotion recognition and the autonomic cycle of intelligent sound analysis (ISA), defined by three tasks: variable extraction, sound data analysis, and emotion recommendation. We include a case study to test our emotion-recognition system in a simulation of a smart movie theater, with different situations taking place. The implementation and verification of the tasks show a promising performance in the case study, with 80% accuracy in sound recognition, and its general behavior shows that it can contribute to improving the well-being of the people present in the environment.

La industria de la construcción representa gran parte del consumo de recursos naturales, proyectándose el aumento de residuos de construcción y demolición (RCD) a nivel mundial en un 70% al año 2050 si no se toman medidas urgentes. La aplicación de economía circular en la construcción, requiere selección de productos en base a su potencial circular, en la fase de diseño, para optimizar la reutilización y minimizar residuos. Se propone metodología mixta combinando componentes cualitativos, explorando cómo los actores involucrados en la construcción integran, múltiples factores que influyen en la recuperación del material, asignándoles valores porcentuales que reflejan el potencial circular del producto. Esta información cuantitativa, se visualizará gráficamente en BIM (Building Information Modeling), obteniendo cuantificación sintética en porcentaje de RCD. Se compara la circularidad del modelo de estudio, con uno más favorable, y otro menos favorable. Detectándose diferencias sustanciales en la circularidad, y una determinación en porcentaje de RCD, para formular diseños informados.

Biogas and hydrogen (H2) are breaking through as alternative energy sources in road transport, specifically for heavy-duty vehicles. Until a public network of service stations is deployed for such vehicles, the owners of large fleets will need to build and manage their own refueling facilities. Fleet refueling management and remote monitoring at these sites will become key business needs. This article describes the construction of a prototype system capable of solving those needs. During the design and development process of the prototype, the standard industry protocols involved in these installations have been considered, and the latest expertise in information technology systems has been applied. This prototype has been essential to determine the Strengths, Challenges, Opportunities and Risks (SCOR) of such a system, which is the first step of a more ambitious project. A second stage will involve setting up a pilot study and developing a commercial system that can be widely installed to provide a real solution for the industry.

Abstract Sequencing of B-cell and T-cell immune receptor repertoires helps us to understand the adaptive immune response, although it only provides information about the clonotypes (lineages) and their frequencies and not about, for example, their affinity or antigen (Ag) specificity. To further characterize the identified clones, usually with special attention to the particularly abundant ones (dominant), additional time-consuming or expensive experiments are generally required. Here, we present an extension of a multiscale model of the germinal center (GC) that we previously developed to gain more insight in B-cell repertoires. We compare the extent that these simulated repertoires deviate from experimental repertoires established from single GCs, blood, or tissue. Our simulations show that there is a limited correlation between clonal abundance and affinity and that there is large affinity variability among same-ancestor (same-clone) subclones. Our simulations suggest that low-abundance clones and subclones, might also be of interest since they may have high affinity for the Ag. We show that the fraction of plasma cells (PCs) with high B-cell receptor (BcR) mRNA content in the GC does not significantly affect the number of dominant clones derived from single GCs by sequencing BcR mRNAs. Results from these simulations guide data interpretation and the design of follow-up experiments.

Abstract Background SARS-CoV-2 infections have a wide spectrum of clinical manifestations whose causes are not completely understood. Some human conditions predispose to severe outcome, like old age or the presence of comorbidities, but many other facets, including coinfections with other viruses, remain poorly characterized. Methods In this study, the eukaryotic fraction of the respiratory virome of 120 COVID-19 patients was characterized through whole metagenomic sequencing. Results Genetic material from respiratory viruses was detected in 25% of all samples, whereas human viruses other than SARS-CoV-2 were found in 80% of them. Samples from hospitalized and deceased patients presented a higher prevalence of different viruses when compared to ambulatory individuals. Small circular DNA viruses from the Anneloviridae (Torque teno midi virus 8, TTV-like mini virus 19 and 26) and Cycloviridae families (Human associated cyclovirus 10), Human betaherpesvirus 6, were found to be significantly more abundant in samples from deceased and hospitalized patients compared to samples from ambulatory individuals. Similarly, Rotavirus A, Measles morbillivirus and Alphapapilomavirus 10 were significantly more prevalent in deceased patients compared to hospitalized and ambulatory individuals. Conclusions Results show the suitability of using metagenomics to characterize a broader peripheric virological landscape of the eukaryotic virome in SARS-CoV-2 infected patients with distinct disease outcomes. Identified prevalent viruses in hospitalized and deceased patients may prove important for the targeted exploration of coinfections that may impact prognosis.

Introduction: Within the organizational culture, the university professor is a key agent for the achievement of learning, coordinating daily the requests of the subjects and majors, together with the students' needs. For this reason, teaching is one of the professions with the highest risk of experiencing stress, as well as anxiety and depression. In order to reduce these factors, programs have been created for teachers with various activities that promote self-care. Objective: To demonstrate the effectiveness of the Meditative Education Program of the Isha Judd System in reducing the levels of stress, depression and anxiety in teachers of the School of Health Sciences of the Universidad Autónoma de Chile. Methods: Ten face-to-face workshops were conducted by facilitators of the Isha Judd System. The participants were divided into three groups that met on Mondays, at different times (n = 22). The meetings were held every 15 days, each session was divided into six moments. In the first and last session, Beck's Depression and Anxiety Test, Rossemberg's Self-Esteem Scale, Perceived Stress Scale and the Isha Judd System wellbeing survey were applied. Results: The stress level of the group decreased by 7.2 points, going from moderate to low, the depression level decreased significantly, as did the anxiety values, reaching mild and minimal. The level of self-esteem was initially in the high range and at the end it remained in the same range, but the average score improved by 1.88 points. Conclusion: In those teachers who met the minimum requirements of the program, this methodology had a positive impact on their satisfaction, improving on average their levels of stress, anxiety, depression and self-esteem. This study has limitations due to the number and type of sample, so a second stage is suggested.

La vegetación ribereña en la cuenca del río Usumacinta en Tabasco, México constituye un ecosistema importante para el mantenimiento de la diversidad biológica, desgraciadamente es el más afectado por la expansión de la ganadería y la agricultura. Entender cómo la diversidad de murciélagos filostómidos se distribuye a lo largo de la franja de vegetación ribereña, es información para la toma de decisiones en la conservación del hábitat de la comunidad de murciélagos. El objetivo del estudio fue comparar la estructura y composición de los ensambles de murciélagos filostómidos en diferentes sitios de vegetación ribereña a lo largo del rio Usumacinta en Tabasco, México. Durante siete meses se realizaron capturas de murciélagos filostómidos con cuatro redes de niebla en seis diferentes sitios con vegetación ribereña. La estructura y composición se describió y comparó entre sitios con base en el número efectivo de especies, curvas-rango abundancia e índice de complementariedad. Se registraron 541 individuos agrupados en 15 especies, el 67% de las especies pertenecen a la subfamilia Stenodermatinae. No se encontraron diferencias entre la riqueza y diversidad de murciélagos filostómidos, sin embargo, se observó diferencias en la composición del ensamble entre sitios. El presente estudio aporta información básica que servirá en la toma de decisiones para conservar la diversidad de murciélagos en vegetación ribereña en el estado y la región.

El pintor surrealista Roberto Matta comenzó su trayectoria artística después de estudiar arquitectura en Chile y trabajar con Le Corbusier en París en los años treinta, periodo en el cual realizó unas singulares ilustraciones residenciales. Este trabajo presenta una revisión e interpretación geométrica de estas imágenes para comprender sus características y posibilidades. Se revisan antecedentes de su realización, y se desarrolla un registro de elementos y modelación paramétrica tridimensional de las formas y organización espacial de las ilustraciones, interpretando su configuración y vinculación con aspectos arquitectónicos. Se elabora una restitución tridimensional basada en la interpretación perspectiva de los elementos, y una programación paramétrica de la geometría de los elementos y ambientes, que permite reconstituir vistas similares a las imágenes, como también generar nuevas vistas, recorridos y configuraciones. La restitución paramétrica de estas imágenes revela posibilidades formales que evidencian una definición espacial relacionada con un cuestionamiento emocional al diseño de las viviendas, expresando una alternativa temprana al desarrollo habitacional moderno.

Abstract Background Patients with juvenile chronic inflammatory systemic diseases (jCID) are vulnerable to many circumstances when transitioning to adult-centered healthcare; this increases the burden of disease and worsen their quality of life. Methods MEDLINE, Embase, Web of Science and Scopus were searched from inception to March 16th, 2021. We included observational, randomized controlled trials and quasi-experimental studies that evaluated a transitional care program for adolescents and young adults with jCIDs. We extracted information regarding health-related quality of life, disease activity, drop-out rates, clinical attendance rates, hospital admission rates, disease-related knowledge, surgeries performed, drug toxicity and satisfaction rates. Results Fifteen studies met our inclusion criteria. The implementation of transition programs showed a reduction on hospital admission rates for those with transition program (OR 0.28; 95% CI 0.13 to 0.61; I 2 = 0%; p = 0.97), rates of surgeries performed (OR 0.26; 95% CI 0.12 to 0.59; I 2 = 0%; p = 0.50) and drop-out rates from the adult clinic (OR 0.23; 95% CI 0.12 to 0.46; I 2 = 0%; p = 0.88). No differences were found in other outcomes. Conclusion The available body of evidence supports the implementation of transition programs as it could be a determining factor to prevent hospital admission rates, surgeries needed and adult clinic attendance rates.

BackgroundAfter the initial outbreak in China (December 2019), the World Health Organization declared COVID-19 a pandemic on March 11th, 2020. This paper aims to describe the first 2 years of the pandemic in Mexico.Design and methodsThis is a population-based longitudinal study. We analyzed data from the national COVID-19 registry to describe the evolution of the pandemic in terms of the number of confirmed cases, hospitalizations, deaths and reported symptoms in relation to health policies and circulating variants. We also carried out logistic regression to investigate the major risk factors for disease severity.ResultsFrom March 2020 to March 2022, the coronavirus disease 2019 (COVID-19) pandemic in Mexico underwent four epidemic waves. Out of 5,702,143 confirmed cases, 680,063 were hospitalized (11.9%), and 324,436 (5.7%) died. Even if there was no difference in susceptibility by gender, males had a higher risk of death (CFP: 7.3 vs. 4.2%) and hospital admission risk (HP: 14.4 vs. 9.5%). Severity increased with age. With respect to younger ages (0–17 years), the 60+ years or older group reached adjusted odds ratios of 9.63 in the case of admission and 53.05 (95% CI: 27.94–118.62) in the case of death. The presence of any comorbidity more than doubled the odds ratio, with hypertension-diabetes as the riskiest combination. While the wave peaks increased over time, the odds ratios for developing severe disease (waves 2, 3, and 4 to wave 1) decreased to 0.15 (95% CI: 0.12–0.18) in the fourth wave.ConclusionThe health policy promoted by the Mexican government decreased hospitalizations and deaths, particularly among older adults with the highest risk of admission and death. Comorbidities augment the risk of developing severe illness, which is shown to rise by double in the Mexican population, particularly for those reported with hypertension-diabetes. Factors such as the decrease in the severity of the SARS-CoV2 variants, changes in symptomatology, and advances in the management of patients, vaccination, and treatments influenced the decrease in mortality and hospitalizations.

Antecedentes: México enfrenta un desafío por la carga que representa la diabetes tipo 2 (DT2). Objetivo: Analizar la epidemiología y la carga de DT2 en México de 1990 a 2021 en los ámbitos nacional y estatal. Material y métodos: Se empleó el Global Burden of Disease 2021 para evaluar prevalencia, incidencia, mortalidad, carga letal y no letal. Se consideraron factores metabólicos, ambientales y de comportamiento. Se realizó análisis comparativo por sexo, edad y entidad federativa. Resultados: Se incrementó la prevalencia de DT2 en 25 % y la incidencia en menores de 45 años; la mortalidad en mujeres disminuyó. La tasa de años de vida saludable (AVISA) perdidos se incrementó en todos los estados, entre 45.2 % en Nuevo León y 237.6 % en Tabasco. En 2021, la DT2 ocasionó 3.1 millones de AVISA perdidos, que representaron 6.6 % de la carga total en México, de la cual 64 % se atribuyó a muertes prematuras. La neuropatía diabética afectó a 47 % y las afecciones visuales a 270 000 personas; 66.3 % de la carga se atribuyó a obesidad. Conclusiones: Urgen políticas integrales para reducir la carga de DT2 en México, mediante pautas estandarizadas, estrategias basadas en evidencia y recursos tecnológicos que mejoren la accesibilidad y eficiencia de la atención médica.

Cold brew coffee (CBC) has gained in popularity due to its distinct sensory experience. However, CBC can pose a risk for bacterial pathogens if not stored properly. High-Pressure Processing (HPP) is a nonthermal technology that can improve the safety of CBC while maintaining its quality. In this study, CBC made from ground roasted coffee grains was processed at 600 MPa for 3 min and stored at 4 or 23 °C for 90 days. The microbiological quality indicators remained stable throughout the study period. Physicochemical and quality parameters, such as pH, total dissolved solids, titratable acidity, color, total phenolic compounds and antioxidant activity, were not significantly affected by HPP. Both unprocessed and HPP CBC samples showed changes in pH, titratable acidity and color stability after 60 days at 23 °C. Unprocessed CBC samples spiked with Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enterica showed decreased counts, but the pathogens were still detectable after 60 days at 4 °C and after 90 days at 23 °C. HPP achieved a >6-log10 reduction in the species tested, with non-detectable levels for at least 90 days at both storage temperatures. These findings suggest that HPP can effectively control vegetative pathogens and spoilage microorganisms in CBC while preserving its quality attributes.

Reportamos el redescubriento del murciélago cara rayada mayor (Vampyrodes major) en el estado de Tabasco, México, después de 60 años. Los registros se basan en dos individuos capturados con redes de niebla dentro del área natural protegida Cañón del Usumacinta, municipio de Tenosique. Éstos registros confirman su procedencia en el estado y representan los registros más recientes en la región sureste de México.

ABSTRACT The COVID-19 disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus started its deadly journey into a global pandemic in Wuhan, China, in December 2019, where it was first isolated. Subsequently, multiple variants of the virus have been identified worldwide. In this review, we discuss the overall landscape of the pandemic in Mexico, including its most prevalent variants, their surveillance at a genomic level, and how they impacted the epidemiology of the disease. We also evaluate the heterologous vaccination in Mexico and how it may have influenced group immunity and helped mitigate the pandemic. Finally, we present an integrated view that could help us to understand the pandemic and serve as an example of the situation in Latin America.

Palladium-promoted indium oxide is a catalyst with potential to realize the large-scale conversion of CO2 into the commodity methanol. This work focuses on the low-cost nickel as an alternative appealing promoter, revealing the atomic-level catalyst design unlocking maximal selectivity and activity.

Abstract Bat acoustic libraries are important tools that assemble echolocation calls to allow the comparison and discrimination to confirm species identifications. The Sonozotz project represents the first nation‐wide library of bat echolocation calls for a megadiverse country. It was assembled following a standardized recording protocol that aimed to cover different recording habitats, recording techniques, and call variation inherent to individuals. The Sonozotz project included 69 species of echolocating bats, a high species richness that represents 50% of bat species found in the country. We include recommendations on how the database can be used and how the sampling methods can be potentially replicated in countries with similar environmental and geographic conditions. To our knowledge, this represents the most exhaustive effort to date to document and compile the diversity of bat echolocation calls for a megadiverse country. This database will be useful to address a range of ecological questions including the effects of anthropogenic activities on bat communities through the analysis of bat sound.

Las enfermedades relacionadas con IgG4 (ER-IgG4) son entidades broin amatorias e inmunomediadas, caracterizadas por la afección multiorgánica, con la formación de pseudotumores que provocan lesión tisular y daño orgánico subsecuente. Se describe el caso de un paciente de 43 años que presentó sialoadenitis esclerosante y cumplió todos los criterios diagnósticos de enfermedad relacionada con IgG4.

A pesar de la extensa red de humedales en el estado de Tabasco y el deterioro de los ecosistemas, existe poca información sobre los patrones de diversidad biológica de los humedales. En el presente estudio, determinamos la diversidad y estructura taxonómica de las aves acuáticas en la laguna Chaschoc en Tabasco, México. De diciembre de 2013 a julio de 2014, realizamos muestreos en 10 puntos de conteo para el registro de las especies de aves acuáticas. Se registraron un total de 3,926 aves pertenecientes a 41 especies, 17 familias y ocho órdenes de aves acuaticas. Adicionalmente, registramos cinco especies de forma no sistemática. Observamos que la mayor riqueza, diversidad y estructura taxonómica de aves acuáticas se presentó en el mes de marzo, cuando la mayoría de las especies migratorias llegaron a la laguna. Nuestros resultados demuestran que este humedal desempeña un papel importante en la conservación de las aves acuáticas ya que alberga el 13% de la avifauna asociada a humedales de México y el 68% de las reportadas en los humedales de Pantanos de Centla. Dichos resultados también reflejan la necesidad de un mayor número de estudios sobre el papel de las lagunas continentales como reservorios de biodiversidad.

Salmonella spp. is a relevant foodborne pathogen with worldwide distribution. To mitigate Salmonella infections, bacteriophages represent an alternative to antimicrobials and chemicals in food animals and food in general. Bacteriophages (phages) are viruses that infect bacteria, which interact constantly with their host. Importantly, the study of these interactions is crucial for the use of phages as a mitigation strategy. In this study, experimental coevolution of Salmonella Enteritidis (S. Enteritidis) and a lytic phage was conducted in tryptic soy broth for 21 days. Transfer to fresh media was conducted daily and every 24 hours, 2 mL of the sample was collected to quantify Salmonella OD600 and phage titter. Additionally, time-shift experiments were conducted on 20 colonies selected on days 1, 12, and 21 to evaluate the evolution of resistance to past (day 1), present (day 12), and future (day 21) phage populations. The behavior of the dynamics was modeled and simulated with mathematical mass-action models. Bacteria and phage from days 1 and 21 were sequenced to determine the emergence of mutations. We found that S. Enteritidis grew for 21 days in the presence and absence of the phage and developed resistance to the phage from day 1. Also, the phage was also able to survive in the media for 21 days, however, the phage titer decreased in approx. 3 logs PFU/mL. The stability of the lytic phage population was consistent with the leaky resistance model. The time-shift experiments showed resistance to phages from day 1 of at least 85% to the past, present, and future phages. Sequencing of S. Enteritidis showed mutations in genes involved in lipopolysaccharide biosynthesis genes rfbP and rfbN at day 21. The phage showed mutations in the tail phage proteins responsible for recognizing the cell surface receptors. These results suggest that interactions between bacteria and phage in a rich resource media generate a rapid resistance to the infective phage but a fraction of the population remains susceptible. Interactions between Salmonella and lytic phages are an important component for the rational use of phages to control this important foodborne pathogen.

ABSTRACT During the coronavirus disease 2019 (COVID-19) pandemic, the emergence and rapid increase of the B.1.1.7 (Alpha) lineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first identified in the United Kingdom in September 2020, was well documented in different areas of the world and became a global public health concern because of its increased transmissibility. The B.1.1.7 lineage was first detected in Mexico during December 2020, showing a slow progressive increase in its circulation frequency, which reached its maximum in May 2021 but never became predominant. In this work, we analyzed the patterns of diversity and distribution of this lineage in Mexico using phylogenetic and haplotype network analyses. Despite the reported increase in transmissibility of the B.1.1.7 lineage, in most Mexican states, it did not displace cocirculating lineages, such as B.1.1.519, which dominated the country from February to May 2021. Our results show that the states with the highest prevalence of B.1.1.7 were those at the Mexico-U.S. border. An apparent pattern of dispersion of this lineage from the northern states of Mexico toward the center or the southeast was observed in the largest transmission chains, indicating possible independent introduction events from the United States. However, other entry points cannot be excluded, as shown by multiple introduction events. Local transmission led to a few successful haplotypes with a localized distribution and specific mutations indicating sustained community transmission. IMPORTANCE The emergence and rapid increase of the B.1.1.7 (Alpha) lineage of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) throughout the world were due to its increased transmissibility. However, it did not displace cocirculating lineages in most of Mexico, particularly B.1.1.519, which dominated the country from February to May 2021. In this work, we analyzed the distribution of B.1.1.7 in Mexico using phylogenetic and haplotype network analyses. Our results show that the states with the highest prevalence of B.1.1.7 (around 30%) were those at the Mexico-U.S. border, which also exhibited the highest lineage diversity, indicating possible introduction events from the United States. Also, several haplotypes were identified with a localized distribution and specific mutations, indicating that sustained community transmission occurred in the country.

Three-dimensional concrete printing is a technique that has been growing constantly, presenting advantages such as reduced completion times and a decreased environmental impact by eliminating the use of formworks. To carry out the process, the printing path of the extruded material and the movement of a robot must be programmed. Thus, the present research simulated these 3D concrete printing processes in a small 2-floor building of 309.06 m2 and then in a 12-floor building of 10,920 m2. To analyze the 3D printing process, discrete-event simulation was used while considering different variables such as extrusion speed and the locations of a robot mounted on tracks. The results show that when comparing the time taken for a conventional construction system to construct concrete walls and the maximum duration for 3D-printed walls, this method is 45% faster than traditional construction for a small building, but for a big building, there is a difference of 40% in favor of conventional construction; however, this was when using only 1 robot for the whole building. After running the same analyses but using 3 robots instead of 1, the total 3D concrete printing time for the big building was 80% faster in favor of the 3D concrete printing process.

El síndrome antisintetasa es una miopatía inflamatoria autoinmune poco frecuente que se caracteriza por la presencia de autoanticuerpos anti sintetasa ARNt (generalmente anti JO1) asociado frecuentemente a miositis, enfermedad pulmonar intersticial, poliartritis, manos de mecánico y fenómeno de Raynaud. Se reporta el caso de una mujer de 45 años que presenta este síndrome con características fenotípicas de dermatomiositis y responde de forma favorable luego de la administración de tratamiento con glucocorticoides asociado a metotrexato.

Aumento del riesgo de infecciones prevenibles y sus complicaciones en pacientes con enfermedades reumáticas inflamatorias crónicas (ERICA), con y sin tratamiento con inmunosupresores (IS), y beneficios de la vacunación.

El mercado externo resulta crucial para el crecimiento competitivo del sector lácteo argentino. Con una demanda mundial cambiante, el sector ha logrado adaptarse e insertarse en nuevos mercados, aunque con un desempeño competitivo muy volátil. Dada la relevancia de estas condiciones de demanda para la competitividad del sector, en el presente trabajo se estudia el posicionamiento de las exportaciones argentinas de Quesos y Leche en Polvo Entera mediante un análisis de la demanda en los principales mercados importadores basado en el enfoque diferencial de la teoría de la producción. Se computan las elasticidades gasto, precio-propio y precio-cruzadas de los lácteos argentinos y de sus competidores; y a partir de dos índices de competitividad se describe las posibilidades competitivas en el mercado de importaciones lácteas.

Purpose: The Omicron subvariant BA.1 of SARS-CoV-2 was first detected in November 2021 and quickly spread worldwide, displacing the Delta variant. In this work, a characterization of the spread of this variant in Mexico is presented. Methods: The time to fixation of BA.1, the diversity of Delta sublineages, the population density, and the level of virus circulation during the inter-wave interval were determined to analyze differences in BA.1 spread. Results: BA.1 began spreading during the first week of December 2021 and became dominant in the next three weeks, causing the fourth COVID-19 epidemiological surge in Mexico. Unlike previous variants, BA.1 did not exhibit a geographically distinct circulation pattern. However, a regional difference in the speed of the replacement of the Delta variant was observed. Conclusions: Viral diversity and the relative abundance of the virus in a particular area around the time of the introduction of a new lineage seem to have influenced the spread dynamics, in addition to population density. Nonetheless, if there is a significant difference in the fitness of the variants, or if the time allowed for the competition is sufficiently long, it seems the fitter virus will eventually become dominant, as observed in the eventual dominance of the BA.1.x variant in Mexico.

Abstract Background In the last decade, increasing evidence has shown that changes in human gut microbiota are associated with diseases, such as obesity. The excreted/secreted proteins (secretome) of the gut microbiota affect the microbial composition, altering its colonization and persistence. Furthermore, it influences microbiota-host interactions by triggering inflammatory reactions and modulating the host's immune response. The metatranscriptome is essential to elucidate which genes are expressed under diseases. In this regard, little is known about the expressed secretome in the microbiome. Here, we use a metatranscriptomic approach to delineate the secretome of the gut microbiome of Mexican children with normal weight (NW) obesity (O) and obesity with metabolic syndrome (OMS). Additionally, we performed the 16S rRNA profiling of the gut microbiota. Results Out of the 115,712 metatranscriptome genes that codified for proteins, 30,024 (26%) were predicted to be secreted, constituting the Secrebiome of the gut microbiome. The 16S profiling confirmed an increased abundance in Firmicutes and decreased in Bacteroidetes in the obesity groups, and a significantly higher richness and diversity than the normal weight group. We found novel biomarkers for obesity with metabolic syndrome such as increased Coriobacteraceae, Collinsela, and Collinsella aerofaciens; Erysipelotrichaceae, Catenibacterium and Catenibacterium sp., and decreased Parabacteroides distasonis, which correlated with clinical and anthropometric parameters associated to obesity and metabolic syndrome. Related to the Secrebiome, 16 genes, homologous to F. prausniitzi, were overexpressed for the obese and 15 genes homologous to Bacteroides, were overexpressed in the obesity with metabolic syndrome. Furthermore, a significant enrichment of CAZy enzymes was found in the Secrebiome. Additionally, significant differences in the antigenic density of the Secrebiome were found between normal weight and obesity groups. Conclusions These findings show, for the first time, the role of the Secrebiome in the functional human-microbiota interaction. Our results highlight the importance of metatranscriptomics to provide novel information about the gut microbiome’s functions that could help us understand the impact of the Secrebiome on the homeostasis of its human host. Furthermore, the metatranscriptome and 16S profiling confirmed the importance of treating obesity and obesity with metabolic syndrome as separate conditions to better understand the interplay between microbiome and disease.

En este artículo la noción de archivo no es abordada en tanto simple forma de registro, sino como una serie de prácticas estéticas y ético-políticas destinadas a incidir en el entramado de la memoria y la historia, que incluso ha llegado a conformarse en paradigma. Mediante un breve recorrido conceptual de corte histórico-genealógico, se sugiere una ruta teórica para interrogarse acerca de las bases éticas y epistemológicas de las prácticas del archivo en el contexto contemporáneo. / In this paper the concept of archive is not treated just as a simple form of register, but rather as a series of aesthetic and ethical-political practices that are aimed to influence upon the memory/history frame-work that has come to conform a paradigm. Through a brief historical-genealogical conceptual trip, a theoretical route is suggested in order to be able to interrogate ourselves about the ethical and epistemological bases of the archive practices in the contemporary context.

Este trabajo tiene como objetivo mostrar las experiencias surgidas a partir del desarrollo de una propuesta de innovación educativa en un centro urbano de la ciudad de Segovia (España). La finalidad de la propuesta es lograr un modelo de aula inclusivo en el que todos los estudiantes se sientan partícipes del proceso de aprendizaje. Para conseguirlo, hemos decidido unir el modelo pedagógico del Aprendizaje cooperativo (AC), junto al trabajo con las Tecnologías de la relación, la información y la comunicación (TRIC). La razón por la que se combinan ambos elementos es el potencial inclusivo que despiertan por separado, así como el interés por este tipo de prácticas educativas. Con el fin de valorar si la propuesta de aula planteada cumple con los objetivos que nos hemos propuesto, se ha llevado a cabo un estudio de caso con el que se pretende comprobar empíricamente su veracidad. Este se elabora a partir de un análisis cualitativo basado en técnicas de observación. Toda la información recogida nos conduce a la divulgación de unas interesantes conclusiones sobre este campo.

Assessing catalytic mechanisms using DFT calculations greatly aids catalyst design, but is impractical for large molecules. Here the authors develop a statistical learning-based thermochemical model for estimating adsorption of organics onto metals, retaining DFT accuracy while reducing the number of calculations by a factor of 20.

Generating and quantifying the effect of the promoter speciation in heterogeneous catalysts is very challenging. Here, the authors show that the precise palladium atoms architecture reached by controlled co-precipitation overcomes selectivity and stability limitations associated with palladium nanoparticles for CO2-based methanol synthesis.

Nowadays, BIM (Building Information Modeling) has steadily been introduced into the construction industry as a promising methodology for managing projects. Therefore, it is necessary to study the users of its processes. Worldwide, there are already systems that classify companies according to how they use BIM, although only a few studies have been conducted on how individual users are classified. Hence, the present study sought to develop a model that characterized BIM users based on several parameters. The methodology employed defined variables based on a literature review, which experts subsequently validated. These variables made it possible to develop the measurement instrument: a structured questionnaire applied to construction professionals. The necessary parameters for the user characterization model were extracted using the Partial Least Squares (PLS) method. This model met the structural evaluation and measurement criteria, which confirmed that it was valid and reliable. It was found that three main aspects mainly characterized users: (i) their use and command of the software and the methodology, (ii) the experience and degree of adoption of the technology, and (iii) the individual’s knowledge of process levels and standardization concerning BIM.

Reconstituted high-density lipoproteins (rHDLs) can transport and specifically release drugs and imaging agents, mediated by the Scavenger Receptor Type B1 (SR-B1) present in a wide variety of tumor cells, providing convenient platforms for developing theranostic systems. Usually, phospholipids or Apo-A1 lipoproteins on the particle surfaces are the motifs used to conjugate molecules for the multifunctional purposes of the rHDL nanoparticles. Cholesterol has been less addressed as a region to bind molecules or functional groups to the rHDL surface. To maximize the efficacy and improve the radiolabeling of rHDL theranostic systems, we synthesized compounds with bifunctional agents covalently linked to cholesterol. This strategy means that the radionuclide was bound to the surface, while the therapeutic agent was encapsulated in the lipophilic core. In this research, HYNIC-S-(CH2)3-S-Cholesterol and DOTA-benzene-p-SC-NH-(CH2)2-NH-Cholesterol derivatives were synthesized to prepare nanoparticles (NPs) of HYNIC-rHDL and DOTA-rHDL, which can subsequently be linked to radionuclides for SPECT/PET imaging or targeted radiotherapy. HYNIC is used to complexing 99mTc and DOTA for labeling molecules with 111, 113mIn, 67, 68Ga, 177Lu, 161Tb, 225Ac, and 64Cu, among others. In vitro studies showed that the NPs of HYNIC-rHDL and DOTA-rHDL maintain specific recognition by SR-B1 and the ability to internalize and release, in the cytosol of cancer cells, the molecules carried in their core. The biodistribution in mice showed a similar behavior between rHDL (without surface modification) and HYNIC-rHDL, while DOTA-rHDL exhibited a different biodistribution pattern due to the significant reduction in the lipophilicity of the modified cholesterol molecule. Both systems demonstrated characteristics for the development of suitable theranostic platforms for personalized cancer treatment.

New 3D-printing technologies allows to make constructive elements, especially walls, faster and with formal diversity. The 3D-printed elements usually have self-supporting capacities, but they need to be reinforced or integrated into larger structures, to make buildings of large extension or height of several floors. This work proposes a residential construction strategy, focused on Chile, which combines a modular main reinforced concrete structure with partitions made of 3D-printed walls to obtain different housing organizations. For this, a structural grid and range of volumes are defined in BIM. In addition, a parametric programming is developed and prototypes of 3D-printed walls are made. The volumetric development provides a wide repertoire of residential surfaces, while the main structure provides a great flexibility of occupancy. The programming organizes the design and execution process, with numeric analysis and visualization capabilities. The executed prototypes demonstrate a constructive feasibility and architectural appealing. This development expresses the possibility to integrate 3D-printing in massive and varied dwelling construction, and suggests new paths for housing construction with the application of new design technologies and automated manufacturing in construction.

Summary: Changes in the human gut microbiome are associated with obesity and metabolic syndrome, but the role of the gut virome in both diseases remains largely unknown. We characterized the gut dsDNA virome of 28 school-aged children with healthy normal-weight (NW, n = 10), obesity (O, n = 10), and obesity with metabolic syndrome (OMS, n = 8), using metagenomic sequencing of virus-like particles (VLPs) from fecal samples. The virome classification confirmed the bacteriophages' dominance, mainly composed of Caudovirales. Notably, phage richness and diversity of individuals with O and OMS tended to increase, while the VLP abundance remained the same among all groups. Of the 4,611 phage contigs composing the phageome, 48 contigs were highly prevalent in ≥80% of individuals, suggesting high inter-individual phage diversity. The abundance of several contigs correlated with gut bacterial taxa; and with anthropometric and biochemical parameters altered in O and OMS. To our knowledge, this gut phageome represents one of the largest datasets and suggests disease-specific phage alterations.

Memory B cells and antibody-secreting plasma cells are generated within germinal centers during affinity maturation in which B-cell proliferation, selection, differentiation, and self-renewal play important roles. The mechanisms behind memory B cell and plasma cell differentiation in germinal centers are not well understood. However, it has been suggested that cell fate is (partially) determined by asymmetric cell division, which involves the unequal distribution of cellular components to both daughter cells. To investigate what level and/or probability of asymmetric segregation of several fate determinant molecules, such as the antigen and transcription factors (BCL6, IRF4, and BLIMP1) recapitulates the temporal switch and DZ-to-LZ ratio in the germinal center, we implemented a multiscale model that combines a core gene regulatory network for plasma cell differentiation with a model describing the cellular interactions and dynamics in the germinal center. Our simulations show that BLIMP1 driven plasma cell differentiation together with coupled asymmetric division of antigen and BLIMP1 with a large segregation between the daughter cells results in a germinal center DZ-to-LZ ratio and a temporal switch from memory B cells to plasma cells that have been observed in experiments.

La construcción con tableros contralaminados de madera (CLT) posee relevantes capacidades de prefabricación y desempeño ambiental, por lo que se ha comenzado a aplicar en algunas edificaciones. Este trabajo analiza las condiciones de modulación arquitectónica de construcciones de pequeño tamaño en CLT, con el fin de determinar estrategias de diseño y de estandarización productiva que fomenten su utilización. Se revisan primero características de los tableros, y luego dimensiones arquitectónicas en una muestra internacional de casos, identificando convergencias en las magnitudes y ordenaciones, así como una versatilidad de diseño y expresión espacial. Estas propiedades revelan la posibilidad de focalizar la producción de tableros en las dimensiones y configuraciones más utilizadas, para facilitar su elaboración y aplicación, como también diversificar disposiciones arquitectónicas para promover esta tecnología constructiva.

En este trabajo se lleva a cabo un análisis detallado de la única novela del escritor guatemalteco Luis de Lión. Al tiempo que se establece su carácter de literatura heterogénea (conforme a la terminología de Cornejo Polar), se explica en qué sentido la proveniencia étnica de su autor implica un elemento subversivo respecto a la literatura indigenista y a la “etnoficción”. Posteriormente se describe su constitución mimética y se establece la importancia de la subjetividad migrante como parte de la propuesta literaria del autor.

Objetivos: golimumab ha sido aprobado para el tratamiento de pacientes con artritis reumatoidea (AR), artritis psoriásica (APs) y espondiloartritis axial. Sin embargo, los datos provenientes de nuestra región son escasos. El objetivo de este estudio fue evaluar la eficacia, seguridad y sobrevida acumulada de golimumab en pacientes de la vida real con AR, APs y espondilitis anquilosante (EA) de diferentes centros de Argentina. Material y métodos: se llevó a cabo un estudio longitudinal, en el que se incluyeron pacientes consecutivos mayores de 18 años con diagnóstico de AR (criterios ACR/EULAR 2010), APs (critertios CASPAR) y Espax (criterios ASAS 2009), que hayan iniciado tratamiento con golimumab de acuerdo a la indicación médica. Se obtuvieron los datos por revisión de historias clínicas. Se consignaron características sociodemográficas, clínicas, comorbilidades y tratamientos previos. Con respecto al golimumab, se registraron fecha de inicio, vía de administración y tratamientos concomitantes. Se determinó la actividad de la enfermedad mediante DAS28 en el caso de la AR, por DAPSA y MDA para APs y por BASDAI en el caso de Espax. Se consignó la presencia de eventos adversos (EA). En el caso de suspensión del tratamiento, se identificaron la fecha y motivo del mismo. Los pacientes fueron seguidos hasta la suspensión del golimumab, pérdida de seguimiento, muerte, o finalización del estudio (30 de noviembre de 2020). Resultados: se incluyeron 182 pacientes, 116 con diagnóstico de AR, 30 con APs y 36 con Espax. La mayoría de ellos (70.9%) eran mujeres con una edad mediana (m) de 55 años (RIC 43.8-64) y una duración de la enfermedad m de 7 años (RIC 4-12.7) al inicio del tratamiento. El 34.6% de los mismos habían recibido al menos una droga modificadora de la enfermedad (DME) biológica (-b) o sintética dirigida (-sd) previamente. El seguimiento total fue de 318.1 pacientes/año. El tratamiento con golimumab mostró mejoría clínica en los tres grupos de pacientes. La incidencia de eventos adversos fue de 6.6 por 100 pacientes/año, siendo las infecciones las más frecuentes. Durante el seguimiento, 50 pacientes (27.5%) suspendieron golimumab, la causa más frecuente fue el fracaso del tratamiento (68%), seguida de la falta de cobertura (16%) y el desarrollo de eventos adversos (10%). La persistencia de golimumab fue del 76% y 68% a los 12 y 24 meses, respectivamente. Se registró una sobrevida de 50.2 meses (IC 95% 44.4-55.9). Los pacientes que habían recibido tratamiento previo con DME-b y/o -sd mostraron una menor sobrevida (HR 2.4, IC 95% 1.3-4.4). Conclusiones: el tratamiento con golimumab en pacientes de la vida real en Argentina ha demostrado una buena eficacia y seguridad. La sobrevida del fármaco fue de más de 4 años y casi el 80% seguía usando golimumab después de un año. El tratamiento previo con otros DME-b o -sd se asoció con una menor sobrevida al tratamiento.

ABSTRACT OBJECTIVE: To assess the determinants of embedded implementation research (EIR) conduct in seven Latin American and Caribbean countries. METHODS: This qualitative interpretative study conducted and analyzed 14 semi-structured interviews based on a grounded theory approach using Atlas-ti© 7.5.7. We grouped the conditions appointed by interviewees as determinants of EIR conduct into six domains. RESULTS: The participation of high-level engaged decision makers as research co-producers is an important EIR determinant that fosters research use. Nevertheless, EIR faces challenges such as dealing with key personnel changes and fluctuating political contexts. CONCLUSIONS: Despite its limitations, EIR is effective in creating a sense of ownership of research results among implementers, which helps bridge the gap between research and decision-making in health systems.

Patients with upper limb disorders are limited in their activities of daily living and impose an important healthcare burden due to the repetitive rehabilitation they require. A way to reduce this burden is through home-based therapy using virtual reality solutions, since they are readily available, provide immersion, and enable accurate motion tracking, and custom applications can be developed for them. However, there is lack of guidelines for the design of effective VR rehabilitation applications in the literature, particularly for bimanual training. This work introduces a VR telerehabilitation system that uses off-the-shelf hardware, a real-time remote setup, and a bimanual training application that aims to improve upper extremity motor function. It is made of six activities and was evaluated by five physiotherapists specialised in (2) neuromotor disorders and (3) functional rehabilitation and occupational therapy. A descriptive analysis of the results obtained from the System Usability Scale test of the application and a collection of qualitative assessments of each game have been carried out. The application obtained a mean score of 86.25 (±8.96 SD) in the System Usability Scale, and the experts concluded that it accurately reproduces activities of daily living movements except for wrist and finger movements. They also offer a set of design guidelines.

Human B-cell differentiation has been extensively investigated on genomic and transcriptomic grounds; however, no studies have accomplished so far detailed analysis of antigen-dependent maturation-associated human B-cell populations from a proteomic perspective. Here, we investigate for the first time the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation using a label-free LC-MS/MS approach applied on 5 purified B-cell subpopulations (naive, centroblasts, centrocytes, memory and plasma B-cells) from human tonsils (data are available via ProteomeXchange with identifier PXD006191). Our results revealed that the actual differences among these B-cell subpopulations are a combination of expression of a few maturation stage-specific proteins within each B-cell subset and maturation-associated changes in relative protein expression levels, which are related with metabolic regulation. The considerable overlap of the proteome of the 5 studied B-cell subsets strengthens the key role of the regulation of the stoichiometry of molecules associated with metabolic regulation and programming, among other signaling cascades (such as antigen recognition and presentation and cell survival) crucial for the transition between each B-cell maturation stage.

Germinal centers play a key role in the adaptive immune system since they are able to produce memory B cells and plasma cells that produce high affinity antibodies for an effective immune protection. The mechanisms underlying cell-fate decisions are not well understood but asymmetric division of antigen, B-cell receptor affinity, interactions between B-cells and T follicular helper cells (triggering CD40 signaling), and regulatory interactions of transcription factors have all been proposed to play a role. In addition, a temporal switch from memory B-cell to plasma cell differentiation during the germinal center reaction has been shown. To investigate if antigen affinity-based Tfh cell help recapitulates the temporal switch we implemented a multiscale model that integrates cellular interactions with a core gene regulatory network comprising BCL6, IRF4, and BLIMP1. Using this model we show that affinity-based CD40 signaling in combination with asymmetric division of B-cells result in switch from memory B-cell to plasma cell generation during the course of the germinal center reaction. We also show that cell fate division is unlikely to be (solely) based on asymmetric division of Ag but that BLIMP1 is a more important factor. Altogether, our model enables to test the influence of molecular modulations of the CD40 signaling pathway on the production of germinal center output cells.

La diversidad de murciélagos en la Reserva de la Biosfera Pantanos de Centla es poco conocida. Con el objetivo de contribuir al conocimiento de este grupo se recopiló información bibliográfica de diferentes fuentes mediante una búsqueda en bases de datos nacionales e internacionales. El ensamble de murciélagos, está conformado por 36 especies, 25 géneros y cinco familias. Sólo tres especies se encuentran enlistadas en la NOM-059-SEMARNAT 2010. El presente listado aporta información básica que servirá en la toma de decisiones para conservar los murciélagos en la Reserva de la Biosfera Pantanos de Centla.

Design and partial implementation of a mobile application that helps bicycle riders flock with other riders while commuting, thus benefiting from the emergent critical mass. Simulations are run to test the model, analysis of their results suggest flock formation is likely given enough users.

Resumen Este artículo analiza las características socioacadémicas de estudiantes de liceos públicos chilenos en contextos de pobreza, que fueron seleccionados por sus profesores como potenciales buenos postulantes a la carrera pedagógica, con aptitud o talento para la pedagogía. El estudio es descriptivo comparativo. Se aplicó un cuestionario a 585 estudiantes de doce liceos. Paralelamente, mediante grupos de discusión, los profesores identificaron previamente 74 con aptitud para la pedagogía y 511 que no la tenían. El cuestionario recogió información sociodemográfica, de rendimiento y de participación social de todos los estudiantes. Luego, los datos se compararon entre los dos grupos a través de chi-cuadrado. Los resultados muestran que los estudiantes seleccionados por sus profesores tienen buenos rendimientos escolares. No hay diferencias significativas por género, nivel socioeconómico ni composición del grupo familiar; sí las hay en atributos como liderazgo estudiantil y participación social. Los resultados muestran que los docentes en servicio destacan el desempeño académico junto con la participación social y el liderazgo, como aspectos que debemos considerar para seleccionar los mejores candidatos para una carrera pedagógica. A partir de los hallazgos de este estudio, se observó que los profesores consideraron a todas sus categorías de selección como un concepto integrado; nunca seleccionaron a sus potenciales estudiantes de pedagogías estimando categorías de atributos personales, capacidades académicas y habilidades de tipo social que fuesen independientes unas de otras.

Objetivos: determinar la frecuencia de enfermedades autoinmunes (EAI) en pacientes con Artritis Reumatoidea (AR) y comparar la frecuencia de EAI entre pacientes con AR y sin AR ni otra EAI reumatológica. Material y Métodos: estudio multicéntrico, observacional, analítico, retrospectivo. Se incluyeron pacientes consecutivos con AR (ACR/EULAR 2010) y como grupo control pacientes con diagnóstico inicial de Osteoartritis primaria (OA).

In this study, we analyzed the sequences of SARS-CoV-2 isolates of the Delta variant in Mexico, which has completely replaced other previously circulating variants in the country due to its transmission advantage. Among all the Delta sublineages that were detected, 81.5 % were classified as AY.20, AY.26, and AY.100. According to publicly available data, these only reached a world prevalence of less than 1%, suggesting a possible Mexican origin. The signature mutations of these sublineages are described herein, and phylogenetic analyses and haplotype networks are used to track their spread across the country. Other frequently detected sublineages include AY.3, AY.62, AY.103, and AY.113. Over time, the main sublineages showed different geographical distributions, with AY.20 predominant in Central Mexico, AY.26 in the North, and AY.100 in the Northwest and South/Southeast. This work describes the circulation, from May to November 2021, of the primary sublineages of the Delta variant associated with the third wave of the COVID-19 pandemic in Mexico and highlights the importance of SARS-CoV-2 genomic surveillance for the timely identification of emerging variants that may impact public health.